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1jq1

POTASSIUM CHANNEL (KCSA) OPEN GATE MODEL

Method: SOLUTION NMR Dmax: 57.4 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

VOLTAGE-GATED POTASSIUM CHANNEL

Streptomyces lividans

UniProt P0A334

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count Chain A； UniProt 86–119 Chain B； UniProt 86–119 Chain C； UniProt 86–119 Chain D； UniProt 86–119 Fragment：INNER TRANSMEMBRANE SEGMENT (residues 86-119) No other associated polymer SOLUTION NMR NMR measurement conditions：pH 7；150 K；Ionic strength (raw mmCIF value) 20 mM CITRATE PHOSPHATE；Pressure 1 NMR measurement conditions：pH 4；150 K；Ionic strength (raw mmCIF value) 20 mM CITRATE PHOSPHATE；Pressure 1 NMR sample composition：1.0 MG/ML MIXED WITH METHANETHIOSULFONATE SPIN LABEL | THE SAMPLES WERE RECONSTITUTED INTO ASOLECTIN LIPOSOMES AT A 1:400 PROTEIN:LIPID RATIO Resolution not provided

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 1JQ1 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1BL8 POTASSIUM CHANNEL (KCSA) FROM STREPTOMYCES LIVIDANS Deposited 1998-07-23 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 23–119（97 aa）
Chain B 23–119（97 aa）
Chain C 23–119（97 aa）
Chain D 23–119（97 aa）
Mutation：L90C Mutation：L90C Mutation：L90C Mutation：L90C K POTASSIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；ONE-TO-MIXTURE OF PROTEIN SOLUTION AND RESERVOIR (200 MM CACL2, 100 MM HEPES PH 7.5, 48% PEG 400).
Resolution 3.20 Å R-free 0.290
1F6G POTASSIUM CHANNEL (KCSA) FULL-LENGTH FOLD Deposited 2000-06-21 Different construct Different mutation/modification Different experimental conditions Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–160（160 aa） Fragment：FULL-LENGTH CHANNEL
Chain B 1–160（160 aa） Fragment：FULL-LENGTH CHANNEL
Chain C 1–160（160 aa） Fragment：FULL-LENGTH CHANNEL
Chain D 1–160（160 aa） Fragment：FULL-LENGTH CHANNEL
Mutation：CYS SCANNING: 5-24, 120-160 Mutation：CYS SCANNING: 5-24, 120-160 Mutation：CYS SCANNING: 5-24, 120-160 Mutation：CYS SCANNING: 5-24, 120-160 No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.2；293 K；Ionic strength (raw mmCIF value) 50-100 mM；Pressure ambient
NMR sample composition 50-100 uM KcsA, PBS pH 7.2, reconstituted into asolectin vesicles at a 1:500 protein:lipid ratio (molar) | 100% H2O
Resolution not provided
1J95 KCSA potassium channel with TBA (tetrabutylammonium) and potassium Deposited 2001-05-23 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–125（125 aa） Fragment：Residues 1-125
Chain B 1–125（125 aa） Fragment：Residues 1-125
Chain C 1–125（125 aa） Fragment：Residues 1-125
Chain D 1–125（125 aa） Fragment：Residues 1-125
Mutation：L90C Mutation：L90C Mutation：L90C Mutation：L90C K POTASSIUM ION × 4 TBA TETRABUTYLAMMONIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG 400, HEPES, POTASSIUM CHLORIDE, CALCIUM CHLORIDE, DETERGENT LDAO, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.80 Å R-free 0.322
1JQ2 POTASSIUM CHANNEL (KCSA) OPEN GATE MODEL Deposited 2001-08-03 Parsed fields agree Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 86–119（34 aa） Fragment：INNER TRANSMEMBRANE SEGMENT (residues 86-119)
Chain B 86–119（34 aa） Fragment：INNER TRANSMEMBRANE SEGMENT (residues 86-119)
Chain C 86–119（34 aa） Fragment：INNER TRANSMEMBRANE SEGMENT (residues 86-119)
Chain D 86–119（34 aa） Fragment：INNER TRANSMEMBRANE SEGMENT (residues 86-119)
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7；150 K；Ionic strength (raw mmCIF value) 20 mM CITRATE PHOSPHATE；Pressure 1
NMR measurement conditions pH 4；150 K；Ionic strength (raw mmCIF value) 20 mM CITRATE PHOSPHATE；Pressure 1
NMR sample composition 1.0 MG/ML MIXED WITH METHANETHIOSULFONATE SPIN LABEL | THE SAMPLES WERE RECONSTITUTED INTO ASOLECTIN LIPOSOMES AT A 1:400 PROTEIN:LIPID RATIO
Resolution not provided
1JVM KCSA POTASSIUM CHANNEL WITH TBA (TETRABUTYLAMMONIUM) AND RUBIDIUM Deposited 2001-08-30 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–125（125 aa） Fragment：Residues 1-125
Chain B 1–125（125 aa） Fragment：Residues 1-125
Chain C 1–125（125 aa） Fragment：Residues 1-125
Chain D 1–125（125 aa） Fragment：Residues 1-125
Mutation：P2A,L90C Mutation：P2A,L90C Mutation：P2A,L90C Mutation：P2A,L90C RB RUBIDIUM ION × 3 TBA TETRABUTYLAMMONIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG400,calcium chloride, Hepes, potassium chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.80 Å R-free 0.302
1JVM KCSA POTASSIUM CHANNEL WITH TBA (TETRABUTYLAMMONIUM) AND RUBIDIUM Deposited 2001-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–125（125 aa） Fragment：Residues 1-125
Chain B 1–125（125 aa） Fragment：Residues 1-125
Chain C 1–125（125 aa） Fragment：Residues 1-125
Chain D 1–125（125 aa） Fragment：Residues 1-125
Mutation：P2A,L90C Mutation：P2A,L90C Mutation：P2A,L90C Mutation：P2A,L90C RB RUBIDIUM ION × 6 TBA TETRABUTYLAMMONIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG400,calcium chloride, Hepes, potassium chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.80 Å R-free 0.302
1K4C Potassium Channel KcsA-Fab complex in high concentration of K+ Deposited 2001-10-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–124（124 aa） Fragment：potassium channel KcsA
Mutation：P2A, L90C F09 NONAN-1-OL × 4 K POTASSIUM ION × 28 DGA DIACYL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.4；298 K；PEG 400, Magnesium Acetate, Sodium Acetate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.00 Å R-free 0.233
1K4D Potassium Channel KcsA-Fab complex in low concentration of K+ Deposited 2001-10-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–124（124 aa） Fragment：potassium channel KcsA
Mutation：P2A, L90C K POTASSIUM ION × 8 NA SODIUM ION × 4 DGA DIACYL GLYCEROL × 4 F09 NONAN-1-OL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.4；298 K；PEG 400,Magnesium Acetate, Sodium Acetate , pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.30 Å R-free 0.235
1R3I potassium channel KcsA-Fab complex in Rb+ Deposited 2003-10-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–124（124 aa）
Mutation：P2A,L90C F09 NONAN-1-OL × 4 RB RUBIDIUM ION × 16 DGA DIACYL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.4；293 K；PEG400, sodium acetate, magnesium acetate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.40 Å R-free 0.236
1R3J potassium channel KcsA-Fab complex in high concentration of Tl+ Deposited 2003-10-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–124（124 aa）
Mutation：P2A,L90C F09 NONAN-1-OL × 4 TL THALLIUM (I) ION × 20 DGA DIACYL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.4；293 K；PEG400, sodium acetate, magnesium acetate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.90 Å R-free 0.228
1R3K potassium channel KcsA-Fab complex in low concentration of Tl+ Deposited 2003-10-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–124（124 aa）
Mutation：P2A,L90C DGA DIACYL GLYCEROL × 4 TL THALLIUM (I) ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.4；293 K；PEG400, sodium acetate, magnesium acetate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.80 Å R-free 0.273
1R3L potassium channel KcsA-Fab complex in Cs+ Deposited 2003-10-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–124（124 aa）
Mutation：P2A,L90C F09 NONAN-1-OL × 4 CS CESIUM ION × 20 DGA DIACYL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.4；293 K；PEG400, sodium acetate, magnesium acetate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.41 Å R-free 0.249
1ZWI Structure of mutant KcsA potassium channel Deposited 2005-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 22–123（102 aa） Fragment：residues 22-123
Not recorded F09 NONAN-1-OL × 1 K POTASSIUM ION × 7 DGA DIACYL GLYCEROL × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å R-free 0.256
2A9H NMR structural studies of a potassium channel / charybdotoxin complex Deposited 2005-07-11 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–132（132 aa）
Chain B 1–132（132 aa）
Chain C 1–132（132 aa）
Chain D 1–132（132 aa）
Mutation：Q58A, T61S, R64D, F103Y, T107F, L110V Mutation：Q58A, T61S, R64D, F103Y, T107F, L110V Mutation：Q58A, T61S, R64D, F103Y, T107F, L110V Mutation：Q58A, T61S, R64D, F103Y, T107F, L110V No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.5；315 K；Pressure ambient
NMR sample composition 1 mM KcsA, 2H and selectively 13C-methyl labeled in 20 mM sodium phosphate (pH 7.5), 5 mM KCl, 1 mM DTT, and 80 mM foscholine-12, 90% H2O/10% D2O or 100% D2O | 90% H2O/10% D2O or 100% D2O
Resolution not provided
2ATK Structure of a mutant KcsA K+ channel Deposited 2005-08-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1–124（124 aa）
Not recorded K POTASSIUM ION × 7 F09 NONAN-1-OL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.4；300 K；PEG400, KCl, MgAcetate, NaAcetate, pH 5.40, VAPOR DIFFUSION, SITTING DROP, temperature 300K
Resolution 2.50 Å R-free 0.261
2BOB Potassium channel KcsA-Fab complex in thallium with tetrabutylammonium (TBA) Deposited 2005-04-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–124（124 aa） Fragment：RESIDUES 1-124
Mutation：YES TL THALLIUM (I) ION × 4 CO COBALT (II) ION × 4 TBA TETRABUTYLAMMONIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.4；PEG 400, MAGNESIUM ACETATE, SODIUM ACETATE, pH 5.40
Resolution 2.76 Å R-free 0.251
2BOC Potassium channel KcsA-Fab complex in thallium with tetraethylarsonium (TEAs) Deposited 2005-04-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–124（124 aa） Fragment：RESIDUES 1-124
Mutation：YES TL THALLIUM (I) ION × 16 CO COBALT (II) ION × 4 T1A TETRAETHYLARSONIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.4；PEG 400, MAGNESIUM ACETATE, SODIUM ACETATE, pH 5.40
Resolution 3.01 Å R-free 0.250
2DWD crystal structure of KcsA-FAB-TBA complex in Tl+ Deposited 2006-08-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 22–124（103 aa） Fragment：residues 22-124
Mutation：L90C F09 NONAN-1-OL × 4 TL THALLIUM (I) ION × 16 L2C (2S)-3-HYDROXY-2-(NONANOYLOXY)PROPYL LAURATE × 4 TBA TETRABUTYLAMMONIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；18-25% PEG 400, 50mM MG(AC)2, 50mM NaAc(pH 5) or Na cacodylate(pH 6) or HEPES(pH 7), pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.60 Å R-free 0.263
2DWE Crystal structure of KcsA-FAB-TBA complex in Rb+ Deposited 2006-08-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 22–124（103 aa） Fragment：residues 22-124
Mutation：L90C RB RUBIDIUM ION × 12 L2C (2S)-3-HYDROXY-2-(NONANOYLOXY)PROPYL LAURATE × 4 F09 NONAN-1-OL × 4 TBA TETRABUTYLAMMONIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；18-25% PEG 400, 50mM MG(AC)2, 50mM NaAc(pH 5) or Na cacodylate(pH 6) or HEPES(pH 7), pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.50 Å R-free 0.266
2HJF Potassium channel kcsa-fab complex with tetrabutylammonium (TBA) Deposited 2006-06-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 22–124（103 aa）
Mutation：L90C K POTASSIUM ION × 16 TBA TETRABUTYLAMMONIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.2；295 K；Cryoprotected to 45% PEG, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 2.90 Å R-free 0.284
2HVJ Crystal structure of KcsA-Fab-TBA complex in low K+ Deposited 2006-07-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–124（124 aa）
Mutation：P2A, L90C F09 NONAN-1-OL × 4 K POTASSIUM ION × 8 L2C (2S)-3-HYDROXY-2-(NONANOYLOXY)PROPYL LAURATE × 4 TBA TETRABUTYLAMMONIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.5；293 K；18-25% PEG 400, 50mM Mg(Ac)2, 50mM NaAc (pH5) or Na Cacodylate (pH6) or Hepes (PH 7), pH 6.5, VAPOR DIFFUSION, temperature 293K
Resolution 2.75 Å R-free 0.246
2HVK crystal structure of the KcsA-Fab-TBA complex in high K+ Deposited 2006-07-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–124（124 aa）
Mutation：P2A, L90C F09 NONAN-1-OL × 4 K POTASSIUM ION × 24 L2C (2S)-3-HYDROXY-2-(NONANOYLOXY)PROPYL LAURATE × 4 TBA TETRABUTYLAMMONIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.5；293 K；18-25% PEG 400, 50mM Mg(Ac)2, 50mM NaAc (pH5) or Na Cacodylate (pH6) or Hepes (PH 7), pH 6.5, VAPOR DIFFUSION, temperature 293K
Resolution 1.90 Å R-free 0.245
2IH1 Ion selectivity in a semi-synthetic K+ channel locked in the conductive conformation Deposited 2006-09-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 3–122（120 aa）
Mutation：S69A, V70C Non-standard monomer：Yes (specific site not provided by mmCIF) K POTASSIUM ION × 20 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.5；293 K；Crystals grown in 150 mM KCl were transferred to a solution containing 1 mM KCl + 149 mM NaCl prior to cryoprotection, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 5.50
Resolution 2.40 Å R-free 0.251
2IH3 Ion selectivity in a semi-synthetic K+ channel locked in the conductive conformation Deposited 2006-09-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 3–122（120 aa）
Mutation：S69A, V70C Non-standard monomer：Yes (specific site not provided by mmCIF) K POTASSIUM ION × 28 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6；293 K；20-25% PEG 400, 50 mM Magnesium acetate, 150 mM KCl, pH 6.0. Crystals were cryoprotected by increasing PEG concentration to 40%, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.72 Å R-free 0.242
2ITC Potassium Channel KcsA-Fab complex in Sodium Chloride Deposited 2006-10-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–124（124 aa） Fragment：residues 1-124
Mutation：L90C NA SODIUM ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；21% PEG400, 50 mM MgAcetate, 150 mM NaCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 3.20 Å R-free 0.265
2ITD Potassium Channel KcsA-Fab complex in Barium Chloride Deposited 2006-10-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–124（124 aa） Fragment：RESIDUES 1-124
Mutation：L90C BA BARIUM ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.2；293 K；21% PEG400, 50 mM MgAcetate, 5 mM BaCl2, 150 mM NaCl, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.70 Å R-free 0.261
2JK5 Potassium Channel KcsA in complex with Tetrabutylammonium in high K Deposited 2008-08-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–124（124 aa） Fragment：RESIDUES 1-124
Not recorded CO COBALT (II) ION × 4 K POTASSIUM ION × 24 F09 NONAN-1-OL × 4 L2C (2S)-3-HYDROXY-2-(NONANOYLOXY)PROPYL LAURATE × 4 TBA TETRABUTYLAMMONIUM ION × 4 HP6 HEPTANE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.4；18-25%PEG400, 50 MM SODIUM ACETATE, 50 MM MAGNESIUM ACETATE, pH 5.4
Resolution 2.40 Å R-free 0.249
2NLJ Potassium Channel KcsA(M96V)-Fab complex in KCl Deposited 2006-10-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–124（124 aa） Fragment：RESIDUES 1-124
Mutation：L90C, M96V DGA DIACYL GLYCEROL × 4 K POTASSIUM ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；PEG400, Magnesium Acetate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.52 Å R-free 0.255
2P7T Crystal Structure of KcsA mutant Deposited 2007-03-20 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain C 22–124（103 aa）
Not recorded K POTASSIUM ION × 24 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；pH 5.2-5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.05 Å R-free 0.234
2P7T Crystal Structure of KcsA mutant Deposited 2007-03-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 22–124（103 aa）
Not recorded F09 NONAN-1-OL × 4 K POTASSIUM ION × 24 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；pH 5.2-5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.05 Å R-free 0.234
2QTO An anisotropic model for potassium channel KcsA Deposited 2007-08-02 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 23–119（97 aa）
Chain B 23–119（97 aa）
Chain C 23–119（97 aa）
Chain D 23–119（97 aa）
Not recorded K POTASSIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；200 MM CACL2, 100 MM HEPES PH 7.5, 48% PEG 400
Resolution 3.20 Å R-free 0.272
2W0F Potassium Channel KcsA-Fab Complex with Tetraoctylammonium Deposited 2008-08-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–124（124 aa） Fragment：RESIDUES 1-124
Not recorded CO COBALT (II) ION × 4 F09 NONAN-1-OL × 4 DGA DIACYL GLYCEROL × 4 HX0 N,N,N-trioctyloctan-1-aminium × 4 K POTASSIUM ION × 28 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.4；18-25% PEG400, 50 MM SODIUM ACETATE, 50 MM MAGNESIUM ACETATE, pH 5.4
Resolution 2.40 Å R-free 0.241
3EFF The Crystal Structure of Full-Length KcsA in its Closed Conformation Deposited 2008-09-08 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain K 22–160（139 aa） Fragment：UNP residues 22-160
Chain L 22–160（139 aa） Fragment：UNP residues 22-160
Chain M 22–160（139 aa） Fragment：UNP residues 22-160
Chain N 22–160（139 aa） Fragment：UNP residues 22-160
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.6；298 K；340 mM (NH4)2SO4, 11% PEG4000, 100 mM Na3C6H5O7 pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 3.80 Å R-free 0.331
3F5W KcsA Potassium channel in the open-inactivated state with 32 A opening at T112 Deposited 2008-11-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 21–124（104 aa）
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q K POTASSIUM ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；25% PEG 400, 50 mM Sodium acetate, 50mM magnesium acetate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 3.30 Å R-free 0.265
3F7V KcsA Potassium channel in the open-inactivated state with 23 A opening at T112 Deposited 2008-11-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 21–124（104 aa）
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q K POTASSIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 3.20 Å R-free 0.276
3F7V KcsA Potassium channel in the open-inactivated state with 23 A opening at T112 Deposited 2008-11-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 21–124（104 aa）
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q K POTASSIUM ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 3.20 Å R-free 0.276
3F7Y KcsA Potassium channel in the partially open state with 17 A opening at T112 Deposited 2008-11-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 21–124（104 aa） Fragment：UNP residues 21-124
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q K POTASSIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 3.40 Å R-free 0.274
3F7Y KcsA Potassium channel in the partially open state with 17 A opening at T112 Deposited 2008-11-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 21–124（104 aa） Fragment：UNP residues 21-124
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 3.40 Å R-free 0.274
3FB5 KcsA potassium channel in the partially open state with 14.5 A opening at T112 Deposited 2008-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 21–124（104 aa） Fragment：UNP residues 21-124
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q K POTASSIUM ION × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, pH 5-6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.80 Å R-free 0.252
3FB5 KcsA potassium channel in the partially open state with 14.5 A opening at T112 Deposited 2008-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 21–124（104 aa） Fragment：UNP residues 21-124
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q K POTASSIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, pH 5-6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.80 Å R-free 0.252
3FB6 KcsA Potassium channel in the partially open state with 16 A opening at T112 Deposited 2008-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 21–124（104 aa） Fragment：UNP residues 21-124
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q K POTASSIUM ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, pH 5-6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 3.00 Å R-free 0.255
3FB6 KcsA Potassium channel in the partially open state with 16 A opening at T112 Deposited 2008-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 21–124（104 aa） Fragment：UNP residues 21-124
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q K POTASSIUM ION × 20 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, pH 5-6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 3.00 Å R-free 0.255
3FB7 Open KcsA potassium channel in the presence of Rb+ ion Deposited 2008-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 21–124（104 aa） Fragment：UNP residues 21-124
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q RB RUBIDIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 3.30 Å R-free 0.255
3FB7 Open KcsA potassium channel in the presence of Rb+ ion Deposited 2008-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 21–124（104 aa） Fragment：UNP residues 21-124
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q RB RUBIDIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 3.30 Å R-free 0.255
3FB8 KcsA Potassium channel in the open-conductive state with 20 A opening at T112 in the presence of Rb+ ion Deposited 2008-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 21–124（104 aa） Fragment：UNP residues 21-124
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q RB RUBIDIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 3.40 Å R-free 0.304
3FB8 KcsA Potassium channel in the open-conductive state with 20 A opening at T112 in the presence of Rb+ ion Deposited 2008-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 21–124（104 aa） Fragment：UNP residues 21-124
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q RB RUBIDIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 3.40 Å R-free 0.304
3GB7 Potassium Channel KcsA-Fab complex in Li+ Deposited 2009-02-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–124（124 aa）
Mutation：P2A, L90C NI NICKEL (II) ION × 4 DGA DIACYL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；24% PEG 400, 100 mM MES, 50 mM Magnesium Acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.85 Å R-free 0.285
3HPL KcsA E71H-F103A mutant in the closed state Deposited 2009-06-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1–124（124 aa） Fragment：UNP residues 1-124
Mutation：L90C,E71H,F103A K POTASSIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG 400, 50 mM magnesium acetate, 50 mM sodium acetate, pH 5.0-6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 3.20 Å R-free 0.269
3IFX Crystal structure of the Spin-labeled KcsA mutant V48R1 Deposited 2009-07-26 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–123（123 aa） Fragment：Pore domain: UNP residues 1-124
Chain B 1–123（123 aa） Fragment：Pore domain: UNP residues 1-124
Chain C 1–123（123 aa） Fragment：Pore domain: UNP residues 1-124
Chain D 1–123（123 aa） Fragment：Pore domain: UNP residues 1-124
Mutation：V48C Mutation：V48C Mutation：V48C Mutation：V48C K POTASSIUM ION × 2 MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 4 TBA TETRABUTYLAMMONIUM ION × 1 Experimental method not declared
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；200 mM CaCl2, 150 mM KCl, 100 mM HEPES, 19-49% PEG 400, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 3.56 Å R-free 0.302
3IFX Crystal structure of the Spin-labeled KcsA mutant V48R1 Deposited 2009-07-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–123（123 aa） Fragment：Pore domain: UNP residues 1-124
Chain B 1–123（123 aa） Fragment：Pore domain: UNP residues 1-124
Chain C 1–123（123 aa） Fragment：Pore domain: UNP residues 1-124
Chain D 1–123（123 aa） Fragment：Pore domain: UNP residues 1-124
Mutation：V48C Mutation：V48C Mutation：V48C Mutation：V48C K POTASSIUM ION × 4 MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 8 TBA TETRABUTYLAMMONIUM ION × 2 Experimental method not declared
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；200 mM CaCl2, 150 mM KCl, 100 mM HEPES, 19-49% PEG 400, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 3.56 Å R-free 0.302
3IGA Potassium Channel KcsA-Fab complex in Li+ and K+ Deposited 2009-07-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–124（124 aa）
Mutation：P2A, L90C NI NICKEL (II) ION × 4 DGA DIACYL GLYCEROL × 4 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.75 Å R-free 0.273
3OGC KcsA E71A variant in presence of Na+ Deposited 2010-08-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 2–124（123 aa） Fragment：UNP RESIDUES 2-124
Mutation：E71A NA SODIUM ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG 400, MgAcetate, MES, pH 6.5, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP
Resolution 3.80 Å R-free 0.283
3OR6 On the structural basis of modal gating behavior in K+channels - E71Q Deposited 2010-09-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 22–124（103 aa） Fragment：UNP RESIDUES 22-124
Mutation：E71Q K POTASSIUM ION × 28 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.70 Å R-free 0.268
3OR7 On the structural basis of modal gating behavior in K+channels - E71I Deposited 2010-09-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 22–124（103 aa） Fragment：UNP RESIDUES 22-124
Mutation：E71I K POTASSIUM ION × 20 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.30 Å R-free 0.271
3PJS Mechanism of Activation Gating in the Full-Length KcsA K+ Channel Deposited 2010-11-10 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain K 22–160（139 aa）
Chain L 22–160（139 aa）
Chain M 22–160（139 aa）
Chain N 22–160（139 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.2M Na/K phosphate, 0.1M Bis-Tris propane pH 7.5, 10% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.80 Å R-free 0.332
3STL KcsA potassium channel mutant Y82C with Cadmium bound Deposited 2011-07-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 22–124（103 aa）
Mutation：Y82C K POTASSIUM ION × 24 CD CADMIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；293 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.40 Å R-free 0.263
3STZ KcsA potassium channel mutant Y82C with nitroxide spin label Deposited 2011-07-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 23–124（102 aa）
Mutation：Y82C MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 4 K POTASSIUM ION × 20 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；293 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.50 Å R-free 0.285
4LBE Structure of KcsA with R122A mutation Deposited 2013-06-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 2–124（123 aa）
Mutation：R122A F09 NONAN-1-OL × 4 DGA DIACYL GLYCEROL × 4 K POTASSIUM ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；PEG 400, MgAcetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP
Resolution 2.75 Å R-free 0.202
4LCU Structure of KcsA with E118A mutation Deposited 2013-06-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 2–124（123 aa）
Not recorded K POTASSIUM ION × 24 DGA DIACYL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；PEG 400, MgAcetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP
Resolution 2.75 Å R-free 0.216
4MSW Y78 ester mutant of KcsA in high K+ Deposited 2013-09-18 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain C 22–76（55 aa） Fragment：UNP residues 22-124,UNP residues 22-124
Chain C 78–124（47 aa） Fragment：UNP residues 22-124,UNP residues 22-124
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) DGA DIACYL GLYCEROL × 4 K POTASSIUM ION × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；PEG400, Magnesium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 2.06 Å R-free 0.229
4UUJ POTASSIUM CHANNEL KCSA-FAB WITH TETRAHEXYLAMMONIUM Deposited 2014-07-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 22–124（103 aa）
Not recorded F09 NONAN-1-OL × 16 IND INDOLE × 4 K POTASSIUM ION × 16 DGA DIACYL GLYCEROL × 4 CO COBALT (II) ION × 4 XA7 TETRAHEXYL AMMONNIUM × 8 PO4 PHOSPHATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.4；CRYSTALLIZATION CONDITIONS: 18-25% PEG400, 50 MM SODIUM REMARK 280 ACETATE, 50 MM MAGNESIUM ACETATE, pH 5.4
Resolution 2.40 Å R-free 0.248
5E1A Structure of KcsA with L24C/R117C mutations Deposited 2015-09-29 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain C 4–124（121 aa） Fragment：UNP residues 4-124
Mutation：L24C, R117C K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.75；293 K；PEG 400, MES, Magnesium Acetate
Resolution 3.40 Å R-free 0.257
5EBL KcsA T75G in the Conductive State Deposited 2015-10-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–125（125 aa） Fragment：UNP residues 1-125
Mutation：T75G F09 NONAN-1-OL × 4 DGA DIACYL GLYCEROL × 4 K POTASSIUM ION × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；PEG 400
Resolution 2.30 Å R-free 0.242
5EBM KcsA T75G mutant in the nonconductive state Deposited 2015-10-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–125（125 aa） Fragment：UNP residues 1-125
Mutation：T75G DGA DIACYL GLYCEROL × 4 F09 NONAN-1-OL × 4 K POTASSIUM ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；PEG 400
Resolution 2.50 Å R-free 0.251
5EBW KcsA with G77ester mutation Deposited 2015-10-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–123（123 aa） Fragment：UNP residues 1-123
Mutation：G77(GOA) Non-standard monomer：Yes (specific site not provided by mmCIF) F09 NONAN-1-OL × 4 DGA DIACYL GLYCEROL × 4 K POTASSIUM ION × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；PEG400
Resolution 2.30 Å R-free 0.249
5EC1 KcsA with V76ester mutation Deposited 2015-10-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–125（125 aa） Fragment：UNP residues 1-125
Mutation：V76ester Non-standard monomer：Yes (specific site not provided by mmCIF) F09 NONAN-1-OL × 4 DGA DIACYL GLYCEROL × 4 K POTASSIUM ION × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；PEG400
Resolution 2.75 Å R-free 0.268
5EC2 KcsA with V76ester+G77dA mutations Deposited 2015-10-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–125（125 aa） Fragment：UNP residues 1-125
Mutation：V76ester,G77dA Non-standard monomer：Yes (specific site not provided by mmCIF) F09 NONAN-1-OL × 4 DGA DIACYL GLYCEROL × 4 K POTASSIUM ION × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；PEG400
Resolution 2.30 Å R-free 0.259
5J9P KcsA in vitro Deposited 2016-04-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 22–117（96 aa） Fragment：UNP residues 22-117
Not recorded K POTASSIUM ION × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG400
Resolution 2.85 Å R-free 0.266
5VK6 Open conformation of KcsA non-inactivating E71A mutant Deposited 2017-04-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 26–121（96 aa）
Not recorded F09 NONAN-1-OL × 4 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 K POTASSIUM ION × 28 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG400, 50 mM magnesium acetate, 50 mM sodium acetate
Resolution 2.25 Å R-free 0.213
5VKE Open conformation of KcsA deep-inactivated Deposited 2017-04-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 26–121（96 aa）
Not recorded F09 NONAN-1-OL × 4 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 K POTASSIUM ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG400, 50 mM magnesium acetate, 50 mM sodium acetate
Resolution 2.37 Å R-free 0.228
5VKH Closed conformation of KcsA Y82A-F103A mutant Deposited 2017-04-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 22–124（103 aa）
Not recorded F09 NONAN-1-OL × 4 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 K POTASSIUM ION × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG400 , 50 mM magnesium acetate, 50 mM sodium acetate
Resolution 2.25 Å R-free 0.203
6NFU Structure of the KcsA-G77A mutant or the 2,4-ion bound configuration of a K+ channel selectivity filter. Deposited 2018-12-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 22–124（103 aa）
Mutation：G77C, L90C F09 NONAN-1-OL × 4 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG400, magnesium acetate, sodium acetate
Resolution 2.09 Å R-free 0.224
6NFV Structure of the KcsA-G77C mutant or the 2,4-ion bound configuration of a K+ channel selectivity filter. Deposited 2018-12-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 22–124（103 aa）
Mutation：G77C, L90C F09 NONAN-1-OL × 4 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG400 , magnesium acetate, sodium acetate
Resolution 2.13 Å R-free 0.243
6PA0 Structure of the G77A mutant in Sodium Chloride Deposited 2019-06-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 22–124（103 aa）
Mutation：G77A, L90C F09 NONAN-1-OL × 4 DGA DIACYL GLYCEROL × 4 NA SODIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；294 K；22-26 % PEG400 (v/v), 50 mM magnesium acetate, 50 mM sodium acetate (pH 4.8.-5.4)
Resolution 2.05 Å R-free 0.233
6W0A Open-gate KcsA soaked in 1 mM BaCl2 Deposited 2020-02-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 28–120（93 aa）
Not recorded BA BARIUM ION × 4 K POTASSIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
Resolution 3.24 Å R-free 0.241
6W0B Open-gate KcsA soaked in 2 mM BaCl2 Deposited 2020-02-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 28–120（93 aa）
Not recorded BA BARIUM ION × 4 K POTASSIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
Resolution 3.60 Å R-free 0.236
6W0C Open-gate KcsA soaked in 4 mM BaCl2 Deposited 2020-02-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 28–120（93 aa）
Not recorded BA BARIUM ION × 4 K POTASSIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
Resolution 3.56 Å R-free 0.248
6W0D Open-gate KcsA soaked in 5 mM BaCl2 Deposited 2020-02-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 28–120（93 aa）
Not recorded BA BARIUM ION × 4 K POTASSIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
Resolution 3.64 Å R-free 0.248
6W0E Open-gate KcsA soaked in 10 mM BaCl2 Deposited 2020-02-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 28–120（93 aa）
Not recorded BA BARIUM ION × 4 K POTASSIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
Resolution 3.51 Å R-free 0.250
6W0F Closed-gate KcsA soaked in 0mM KCl/5mM BaCl2 Deposited 2020-02-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 22–124（103 aa）
Not recorded K POTASSIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
Resolution 2.40 Å R-free 0.270
6W0G Closed-gate KcsA soaked in 1mM KCl/5mM BaCl2 Deposited 2020-02-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 22–124（103 aa）
Mutation：L90C K POTASSIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
Resolution 2.60 Å R-free 0.245
6W0H Closed-gate KcsA soaked in 5mM KCl/5mM BaCl2 Deposited 2020-02-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 22–124（103 aa）
Mutation：L90C K POTASSIUM ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
Resolution 2.60 Å R-free 0.239
6W0I Closed-gate KcsA soaked in 10mM KCl/5mM BaCl2 Deposited 2020-02-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 22–124（103 aa）
Mutation：L90C K POTASSIUM ION × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
Resolution 2.33 Å R-free 0.232
6W0J Closed-gate KcsA incubated in BaCl2/NaCl Deposited 2020-02-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 22–124（103 aa）
Mutation：L90C BA BARIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
Resolution 2.50 Å R-free 0.218
7M2H Structural Snapshots of Intermediates in the Gating of a K+ Channel Deposited 2021-03-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 3–125（123 aa）
Not recorded F09 NONAN-1-OL × 4 K POTASSIUM ION × 24 DGA DIACYL GLYCEROL × 4 TBA TETRABUTYLAMMONIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.25；293 K；50 mM MES, pH 6.25, 28% PEG400, 50 mM magnesium acetate
Resolution 2.64 Å R-free 0.268
7M2H Structural Snapshots of Intermediates in the Gating of a K+ Channel Deposited 2021-03-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain F 3–125（123 aa）
Not recorded F09 NONAN-1-OL × 4 K POTASSIUM ION × 24 DGA DIACYL GLYCEROL × 4 TBA TETRABUTYLAMMONIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.25；293 K；50 mM MES, pH 6.25, 28% PEG400, 50 mM magnesium acetate
Resolution 2.64 Å R-free 0.268
7M2I Structural Snapshots of Intermediates in the Gating of a K+ Channel Deposited 2021-03-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 26–116（91 aa）
Not recorded F09 NONAN-1-OL × 4 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 K POTASSIUM ION × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；50 mM MES, pH 6.25, 28% PEG400, 50 mM magnesium acetate
Resolution 2.69 Å R-free 0.251
7M2J Structural Snapshots of Intermediates in the Gating of a K+ Channel Deposited 2021-03-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 26–116（91 aa）
Not recorded K POTASSIUM ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；33% PEG400, 100 mM MES, pH 6.5, 100 mM calcium chloride
Resolution 3.20 Å R-free 0.305
7MDJ The structure of KcsA in complex with a synthetic Fab Deposited 2021-04-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–124（124 aa）
Not recorded K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；50 mM sodium acetate/50 mM ammonium acetate, 50 mM magnesium acetate, 25% PEG 400.
Resolution 2.75 Å R-free 0.265
7MHR KcsA E71V closed gate with K+ Deposited 2021-04-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 3–124（122 aa）
Mutation：E71V, L90C K POTASSIUM ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；50 mM sodium acetate pH 5.5, 50 mM magnesium acetate, 25% PEG 400
Resolution 2.77 Å R-free 0.203
7MHX KcsA E71V closed gate with Ba2+ Deposited 2021-04-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 2–124（123 aa）
Mutation：P2A, E71V, L90C BA BARIUM ION × 4 DGA DIACYL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；50 mM sodium acetate pH 5.5, 50 mM magnesium acetate, 25% PEG 400
Resolution 2.85 Å R-free 0.244
7MJT KcsA open gate E71V mutant with Barium Deposited 2021-04-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 26–121（96 aa）
Mutation：A28C, E71V, L90C, R117Q, E118C, E120Q, R121Q BA BARIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；50 mM sodium/ammonium acetate pH 5.5, 50 mM Magnesium acetate, 25% PG 400
Resolution 3.30 Å R-free 0.270
7MK6 KcsA open gate E71V mutant with sodium Deposited 2021-04-21 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 26–121（96 aa）
Mutation：A28C, E71V, L90C, R117Q, E118C, E120Q, R121Q No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；50 mM sodium/ammonium acetate pH 5.5, 50 mM Magnesium acetate, 25% PEG 400
Resolution 3.10 Å R-free 0.253
7MUB KcsA Open gate E71V mutant in Potassium Deposited 2021-05-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 26–121（96 aa）
Mutation：A28C, E71V, L90C, R117Q, E118C, E120Q, R121Q K POTASSIUM ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；50 mM Sodium acetate/Ammonium acetate, 50 mM Magnesium acetate, 25% PEG 400
Resolution 3.00 Å R-free 0.260
7RP0 Structural Snapshots of Intermediates in the Gating of a K+ Channel Deposited 2021-08-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 22–124（103 aa） Fragment：UNP residues 22-124
Mutation：Y82A F09 NONAN-1-OL × 4 K POTASSIUM ION × 16 TBA TETRABUTYLAMMONIUM ION × 4 DGA DIACYL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.25；293 K；50 mM MES, pH 6.25, 50 mM magnesium acetate, 27% PEG400
Resolution 2.48 Å R-free 0.271
8THN KcsA M96V mutant with Y78ester in High K+ Deposited 2023-07-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 22–124（103 aa）
Mutation：M96V, Y78ester Non-standard monomer：Yes (specific site not provided by mmCIF) K POTASSIUM ION × 4 TBA TETRABUTYLAMMONIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.25；291 K；27% PEG 400 (v/v), 50 mM magnesium acetate, 50 mM MES pH 6.25
Resolution 2.90 Å R-free 0.276

86 other PDB entries and 96 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name KCSA_STRLI
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–34； UniProt 86–119 Author chain B； PDBConstruct 1–34； UniProt 86–119 Author chain C； PDBConstruct 1–34； UniProt 86–119 Author chain D； PDBConstruct 1–34； UniProt 86–119

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 1jq1

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 1jq1
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2. Structure Basics 2. Structure Basics

Entry ID entry_id1jq1
Deposition date deposition_date2001-08-03
Structure title titlePOTASSIUM CHANNEL (KCSA) OPEN GATE MODEL
Keywords keywordsPOTASSIUM CHANNEL, INTEGRAL MEMBRANE PROTEIN, OPEN STATE, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method methodSOLUTION NMR
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier18.05 Å
Radius of gyration Rg (electron density) rg_electron17.57 Å
Forward intensity I(0) i03148540.00
Molecular weight molecular_weight14437.0 kDa
Excluded volume excluded_volume18210 ų
Envelope volume envelope_volume11471 ų
Hydration-shell volume shell_volume7171 ų
Envelope diameter envelope_diameter56.3 Å
Shell Rg shell_rg18.95 Å
Envelope Rg envelope_rg16.48 Å
Shape Rg shape_rg16.90 Å
Total Rg total_rg17.77 Å
Total atoms total_atoms—
Residues n_residues—
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax57.4 Å
Rg (real space) rg_real18.01 Å
Rg uncertainty (real space) rg_real_error0.25 Å
I(0) (real space) i0_real3.1490e+06
I(0) uncertainty (real space) i0_real_error3.7680e+04
Rg (reciprocal space) rg_reciprocal18.02 Å
I(0) (reciprocal space) i0_reciprocal3149000.0000
Solution quality estimate total_estimate0.8972
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary21.7 Å
Skewness Skewness skewness0.209
Kurtosis Kurtosis kurtosis-0.328
Angular range angular_range— – 0.4400 Å−1
Current regularization parameter α current_alpha0.0037
Highest regularization parameter α highest_alpha182700.0000
Real-space data points n_real_points75
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.915; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.914
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (1)

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7. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (4 domains)

Domain ID domain_idd1jq1a_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.14 — Gated ion channels
Superfamily Superfamily superfamilyf.14.1 — Voltage-gated ion channels
Family Family familyf.14.1.1 — Voltage-gated potassium channels
Domain ID domain_idd1jq1b_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.14 — Gated ion channels
Superfamily Superfamily superfamilyf.14.1 — Voltage-gated ion channels
Family Family familyf.14.1.1 — Voltage-gated potassium channels
Domain ID domain_idd1jq1c_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.14 — Gated ion channels
Superfamily Superfamily superfamilyf.14.1 — Voltage-gated ion channels
Family Family familyf.14.1.1 — Voltage-gated potassium channels
Domain ID domain_idd1jq1d_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.14 — Gated ion channels
Superfamily Superfamily superfamilyf.14.1 — Voltage-gated ion channels
Family Family familyf.14.1.1 — Voltage-gated potassium channels
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8. Citations (4)

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9. Files and Curves (10)