1jup

Crystal structure of the multidrug binding transcriptional repressor QacR bound to malachite green

Method: X-RAY DIFFRACTION Dmax: 116.2 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 1jup

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1jup
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1jup
沉积日期 deposition_date2001-08-24
结构标题 titleCrystal structure of the multidrug binding transcriptional repressor QacR bound to malachite green
关键词 keywordsmultidrug recognition, malachite green, QacR, multidrug binding protein, TRANSCRIPTION; TRANSCRIPTION
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier35.77
回转半径 Rg (电子) rg_electron35.38
零角强度 I(0) i0123042000.00
分子量 molecular_weight89846.0 kDa
排除体积 excluded_volume112590 ų
包络体积 envelope_volume144490 ų
水化壳体积 shell_volume35111 ų
包络直径 envelope_diameter117.2
壳层 Rg shell_rg40.48
包络 Rg envelope_rg34.72
形状 Rg shape_rg35.35
总 Rg total_rg35.83
总原子数 total_atoms6323
残基数 n_residues744
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax116.2
Rg (实空间) rg_real35.94
Rg 误差 (实空间) rg_real_error1.28
I(0) (实空间) i0_real1.2300e+08
I(0) 误差 (实空间) i0_real_error2.2950e+06
Rg (倒空间) rg_reciprocal35.84
I(0) (倒空间) i0_reciprocal123000000.0000
解质量估计 total_estimate0.8502
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks1
主峰位置 r_peak_primary31.7
偏度 Skewness skewness0.341
峰度 Kurtosis kurtosis-0.752
角度范围 angular_range— – 0.2200 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha24930000.0000
实空间数据点数 n_real_points45
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.821; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.822; Smooth: 0.763

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (4)

6. 折叠分类 (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

结构域编号 domain_idd1jupa1
类 Class classa — All alpha proteins
折叠类型 Fold folda.4 — DNA/RNA-binding 3-helical bundle
超家族 Superfamily superfamilya.4.1 — Homeodomain-like
家族 Family familya.4.1.9 — Tetracyclin repressor-like, N-terminal domain
结构域编号 domain_idd1jupa2
类 Class classa — All alpha proteins
折叠类型 Fold folda.121 — Tetracyclin repressor-like, C-terminal domain
超家族 Superfamily superfamilya.121.1 — Tetracyclin repressor-like, C-terminal domain
家族 Family familya.121.1.1 — Tetracyclin repressor-like, C-terminal domain
结构域编号 domain_idd1jupb1
类 Class classa — All alpha proteins
折叠类型 Fold folda.4 — DNA/RNA-binding 3-helical bundle
超家族 Superfamily superfamilya.4.1 — Homeodomain-like
家族 Family familya.4.1.9 — Tetracyclin repressor-like, N-terminal domain
结构域编号 domain_idd1jupb2
类 Class classa — All alpha proteins
折叠类型 Fold folda.121 — Tetracyclin repressor-like, C-terminal domain
超家族 Superfamily superfamilya.121.1 — Tetracyclin repressor-like, C-terminal domain
家族 Family familya.121.1.1 — Tetracyclin repressor-like, C-terminal domain
结构域编号 domain_idd1jupd1
类 Class classa — All alpha proteins
折叠类型 Fold folda.4 — DNA/RNA-binding 3-helical bundle
超家族 Superfamily superfamilya.4.1 — Homeodomain-like
家族 Family familya.4.1.9 — Tetracyclin repressor-like, N-terminal domain
结构域编号 domain_idd1jupd2
类 Class classa — All alpha proteins
折叠类型 Fold folda.121 — Tetracyclin repressor-like, C-terminal domain
超家族 Superfamily superfamilya.121.1 — Tetracyclin repressor-like, C-terminal domain
家族 Family familya.121.1.1 — Tetracyclin repressor-like, C-terminal domain
结构域编号 domain_idd1jupe1
类 Class classa — All alpha proteins
折叠类型 Fold folda.4 — DNA/RNA-binding 3-helical bundle
超家族 Superfamily superfamilya.4.1 — Homeodomain-like
家族 Family familya.4.1.9 — Tetracyclin repressor-like, N-terminal domain
结构域编号 domain_idd1jupe2
类 Class classa — All alpha proteins
折叠类型 Fold folda.121 — Tetracyclin repressor-like, C-terminal domain
超家族 Superfamily superfamilya.121.1 — Tetracyclin repressor-like, C-terminal domain
家族 Family familya.121.1.1 — Tetracyclin repressor-like, C-terminal domain

CATH v4.4 (8 domains)

结构域编号 domain_id1jupA01
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily60 — Homeodomain-like
结构域编号 domain_id1jupA02
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology357 — Tetracycline Repressor; domain 2
同源超家族 H-superfamily homologous superfamily10 — Tetracycline Repressor, domain 2
结构域编号 domain_id1jupB01
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily60 — Homeodomain-like
结构域编号 domain_id1jupB02
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology357 — Tetracycline Repressor; domain 2
同源超家族 H-superfamily homologous superfamily10 — Tetracycline Repressor, domain 2
结构域编号 domain_id1jupD01
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily60 — Homeodomain-like
结构域编号 domain_id1jupD02
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology357 — Tetracycline Repressor; domain 2
同源超家族 H-superfamily homologous superfamily10 — Tetracycline Repressor, domain 2
结构域编号 domain_id1jupE01
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily60 — Homeodomain-like
结构域编号 domain_id1jupE02
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology357 — Tetracycline Repressor; domain 2
同源超家族 H-superfamily homologous superfamily10 — Tetracycline Repressor, domain 2

7. 引用文献 (1)

8. 文件与曲线 (10)