1wcq

Mutagenesis of the Nucleophilic Tyrosine in a Bacterial Sialidase to Phenylalanine.

Method: X-RAY DIFFRACTION Dmax: 171.4 Å Quality: REASONABLE

SAXS 散射曲线 SAXS Profile

SAXS profile for 1wcq

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1wcq
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1wcq
沉积日期 deposition_date2004-11-19
结构标题 titleMutagenesis of the Nucleophilic Tyrosine in a Bacterial Sialidase to Phenylalanine.
关键词 keywordsHYDROLASE, SIALIDASE, MICROMONOSPORA VIRIDIFACIENS, HYDROLASE NEURAMINIDASE, GLYCOSIDASE; HYDROLASE
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier51.88
回转半径 Rg (电子) rg_electron52.23
零角强度 I(0) i0604230000.00
分子量 molecular_weight194040.0 kDa
排除体积 excluded_volume239040 ų
包络体积 envelope_volume334580 ų
水化壳体积 shell_volume59618 ų
包络直径 envelope_diameter182.7
壳层 Rg shell_rg47.85
包络 Rg envelope_rg51.14
形状 Rg shape_rg52.27
总 Rg total_rg51.96
总原子数 total_atoms13694
残基数 n_residues1801
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax171.4
Rg (实空间) rg_real52.33
Rg 误差 (实空间) rg_real_error2.26
I(0) (实空间) i0_real6.0420e+08
I(0) 误差 (实空间) i0_real_error1.2440e+07
Rg (倒空间) rg_reciprocal51.49
I(0) (倒空间) i0_reciprocal603500000.0000
解质量估计 total_estimate0.5698
解质量评级 solution_quality REASONABLE a REASONABLE solution
P(r) 峰数 n_peaks1
主峰位置 r_peak_primary45.7
偏度 Skewness skewness0.537
峰度 Kurtosis kurtosis-0.354
角度范围 angular_range— – 0.1500 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha28260000.0000
实空间数据点数 n_real_points31
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.814; Stabil: 0.999; Sysdev: 0.007; Positv: 1.000; Valcen: 0.887; Smooth: 0.056

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (5)

6. 折叠分类 (SCOP + CATH) 18 domains

SCOP 2.08 (9 domains)

结构域编号 domain_idd1wcqa1
类 Class classb — All beta proteins
折叠类型 Fold foldb.1 — Immunoglobulin-like beta-sandwich
超家族 Superfamily superfamilyb.1.18 — E set domains
家族 Family familyb.1.18.0 — automated matches
结构域编号 domain_idd1wcqa2
类 Class classb — All beta proteins
折叠类型 Fold foldb.18 — Galactose-binding domain-like
超家族 Superfamily superfamilyb.18.1 — Galactose-binding domain-like
家族 Family familyb.18.1.0 — automated matches
结构域编号 domain_idd1wcqa3
类 Class classb — All beta proteins
折叠类型 Fold foldb.68 — 6-bladed beta-propeller
超家族 Superfamily superfamilyb.68.1 — Sialidases
家族 Family familyb.68.1.1 — Sialidases (neuraminidases)
结构域编号 domain_idd1wcqb1
类 Class classb — All beta proteins
折叠类型 Fold foldb.1 — Immunoglobulin-like beta-sandwich
超家族 Superfamily superfamilyb.1.18 — E set domains
家族 Family familyb.1.18.0 — automated matches
结构域编号 domain_idd1wcqb2
类 Class classb — All beta proteins
折叠类型 Fold foldb.18 — Galactose-binding domain-like
超家族 Superfamily superfamilyb.18.1 — Galactose-binding domain-like
家族 Family familyb.18.1.0 — automated matches
结构域编号 domain_idd1wcqb3
类 Class classb — All beta proteins
折叠类型 Fold foldb.68 — 6-bladed beta-propeller
超家族 Superfamily superfamilyb.68.1 — Sialidases
家族 Family familyb.68.1.1 — Sialidases (neuraminidases)
结构域编号 domain_idd1wcqc1
类 Class classb — All beta proteins
折叠类型 Fold foldb.1 — Immunoglobulin-like beta-sandwich
超家族 Superfamily superfamilyb.1.18 — E set domains
家族 Family familyb.1.18.0 — automated matches
结构域编号 domain_idd1wcqc2
类 Class classb — All beta proteins
折叠类型 Fold foldb.18 — Galactose-binding domain-like
超家族 Superfamily superfamilyb.18.1 — Galactose-binding domain-like
家族 Family familyb.18.1.0 — automated matches
结构域编号 domain_idd1wcqc3
类 Class classb — All beta proteins
折叠类型 Fold foldb.68 — 6-bladed beta-propeller
超家族 Superfamily superfamilyb.68.1 — Sialidases
家族 Family familyb.68.1.1 — Sialidases (neuraminidases)

CATH v4.4 (9 domains)

结构域编号 domain_id1wcqA01
类 Class class2 — Mainly Beta
架构 Architecture architecture120 — 6 Propeller
拓扑 Topology topology10 — Neuraminidase
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id1wcqA02
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily10 — Immunoglobulins
结构域编号 domain_id1wcqA03
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily260 — Galactose-binding domain-like
结构域编号 domain_id1wcqB01
类 Class class2 — Mainly Beta
架构 Architecture architecture120 — 6 Propeller
拓扑 Topology topology10 — Neuraminidase
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id1wcqB02
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily10 — Immunoglobulins
结构域编号 domain_id1wcqB03
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily260 — Galactose-binding domain-like
结构域编号 domain_id1wcqC01
类 Class class2 — Mainly Beta
架构 Architecture architecture120 — 6 Propeller
拓扑 Topology topology10 — Neuraminidase
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id1wcqC02
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily10 — Immunoglobulins
结构域编号 domain_id1wcqC03
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily260 — Galactose-binding domain-like

7. 引用文献 (1)

8. 文件与曲线 (10)