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2che

STRUCTURE OF THE MG2+-BOUND FORM OF CHEY AND MECHANISM OF PHOSPHORYL TRANSFER IN BACTERIAL CHEMOTAXIS

Method: X-RAY DIFFRACTION Dmax: 44.7 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

CHEY

Salmonella typhimurium

UniProt P0A2D5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 1–128 Not recorded MG MAGNESIUM ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 1.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 2CHE Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2CHF STRUCTURE OF THE MG2+-BOUND FORM OF CHEY AND THE MECHANISM OF PHOSPHORYL TRANSFER IN BACTERIAL CHEMOTAXIS Deposited 1994-01-17 Different ligand/ion Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–128（128 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å
2CHY THREE-DIMENSIONAL STRUCTURE OF CHEY, THE RESPONSE REGULATOR OF BACTERIAL CHEMOTAXIS Deposited 1990-05-17 Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–128（128 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.70 Å
2FKA Crystal structure of Mg(2+) and BeF(3)(-)-bound CheY in complex with CheZ(200-214) solved from a F432 crystal grown in CAPS (pH 10.5) Deposited 2006-01-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 1 SO4 SULFATE ION × 1 CXS 3-CYCLOHEXYL-1-PROPYLSULFONIC ACID × 2 BEF BERYLLIUM TRIFLUORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10.5；298 K；2M ammonium sulfate, 0.2M lithium sulfate, 0.1M CAPS, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.00 Å R-free 0.224
2FKA Crystal structure of Mg(2+) and BeF(3)(-)-bound CheY in complex with CheZ(200-214) solved from a F432 crystal grown in CAPS (pH 10.5) Deposited 2006-01-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric
Chain A 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 12 SO4 SULFATE ION × 12 CXS 3-CYCLOHEXYL-1-PROPYLSULFONIC ACID × 24 BEF BERYLLIUM TRIFLUORIDE ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10.5；298 K；2M ammonium sulfate, 0.2M lithium sulfate, 0.1M CAPS, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.00 Å R-free 0.224
2FLK Crystal structure of CheY in complex with CheZ(200-214) solved from a F432 crystal grown in CAPS (pH 10.5) Deposited 2006-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–128（128 aa）
Not recorded SO4 SULFATE ION × 1 CXS 3-CYCLOHEXYL-1-PROPYLSULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10.5；298 K；2M ammonium sulfate, 0.2M lithium sulfate, 0.1M CAPS, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.10 Å R-free 0.207
2FLK Crystal structure of CheY in complex with CheZ(200-214) solved from a F432 crystal grown in CAPS (pH 10.5) Deposited 2006-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric
Chain A 1–128（128 aa）
Not recorded SO4 SULFATE ION × 24 CXS 3-CYCLOHEXYL-1-PROPYLSULFONIC ACID × 48 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10.5；298 K；2M ammonium sulfate, 0.2M lithium sulfate, 0.1M CAPS, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.10 Å R-free 0.207
2FLW Crystal structure of Mg2+ and BeF3- ound CheY in complex with CheZ 200-214 solved from a F432 crystal grown in Hepes (pH 7.5) Deposited 2006-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 1 SO4 SULFATE ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；2M ammonium sulfate, 0.2M lithium sulfate, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.00 Å R-free 0.228
2FMF Crystal structure of CheY in complex with CheZ 200-214 solved from a F432 crystal grown in Hepes (pH 7.5) Deposited 2006-01-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–128（128 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；2M ammonium sulfate, 0.2M lithium sulfate, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.00 Å R-free 0.213
2FMH Crystal structure of Mg2+ and BeF3- bound CheY in complex with CheZ 200-214 solved from a F432 crystal grown in Tris (pH 8.4) Deposited 2006-01-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 1 SO4 SULFATE ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；2M ammonium sulfate, 0.2M lithium sulfate, 0.1M Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.00 Å R-free 0.246
2FMH Crystal structure of Mg2+ and BeF3- bound CheY in complex with CheZ 200-214 solved from a F432 crystal grown in Tris (pH 8.4) Deposited 2006-01-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric
Chain A 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 24 SO4 SULFATE ION × 24 BEF BERYLLIUM TRIFLUORIDE ION × 24 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 24 GOL GLYCEROL × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；2M ammonium sulfate, 0.2M lithium sulfate, 0.1M Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.00 Å R-free 0.246
2FMH Crystal structure of Mg2+ and BeF3- bound CheY in complex with CheZ 200-214 solved from a F432 crystal grown in Tris (pH 8.4) Deposited 2006-01-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 3 SO4 SULFATE ION × 3 BEF BERYLLIUM TRIFLUORIDE ION × 3 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 3 GOL GLYCEROL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；2M ammonium sulfate, 0.2M lithium sulfate, 0.1M Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.00 Å R-free 0.246
2FMI Crystal structure of CheY in complex with CheZ 200-214 solved from a F432 crystal grown in Tris (pH 8.4) Deposited 2006-01-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–128（128 aa）
Not recorded SO4 SULFATE ION × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；2M ammonium sulfate, 0.2M lithium sulfate, 0.1M Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.30 Å R-free 0.219
2FMI Crystal structure of CheY in complex with CheZ 200-214 solved from a F432 crystal grown in Tris (pH 8.4) Deposited 2006-01-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–128（128 aa）
Not recorded SO4 SULFATE ION × 3 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 3 GOL GLYCEROL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；2M ammonium sulfate, 0.2M lithium sulfate, 0.1M Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.30 Å R-free 0.219
2FMK Crystal structure of Mg2+ and BeF3- bound CheY in complex with CheZ 200-214 solved from a P2(1)2(1)2 crystal grown in MES (pH 6.0) Deposited 2006-01-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；37.5% PEG 8000, 0.05M sodium phosphate (monobasic), 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.00 Å R-free 0.232
2PL9 Crystal Structure of CheY-Mg(2+)-BeF(3)(-) in Complex with CheZ(C19) Peptide solved from a P2(1)2(1)2 Crystal Deposited 2007-04-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 2–129（128 aa）
Not recorded MG MAGNESIUM ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；37.5 % PEG 8000, 0.1 M ammonium thiocyanate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.60 Å R-free 0.274
2PL9 Crystal Structure of CheY-Mg(2+)-BeF(3)(-) in Complex with CheZ(C19) Peptide solved from a P2(1)2(1)2 Crystal Deposited 2007-04-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 2–129（128 aa）
Not recorded MG MAGNESIUM ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；37.5 % PEG 8000, 0.1 M ammonium thiocyanate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.60 Å R-free 0.274
2PL9 Crystal Structure of CheY-Mg(2+)-BeF(3)(-) in Complex with CheZ(C19) Peptide solved from a P2(1)2(1)2 Crystal Deposited 2007-04-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 2–129（128 aa）
Not recorded MG MAGNESIUM ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；37.5 % PEG 8000, 0.1 M ammonium thiocyanate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.60 Å R-free 0.274
2PMC Crystal Structure of CheY-Mg(2+) in Complex with CheZ(C15) Peptide solved from a P1 Crystal Deposited 2007-04-20 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 2–129（128 aa）
Not recorded MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；37.5 % PEG 8000, 0.1 M ammonium thiocyanate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.69 Å R-free 0.285
2PMC Crystal Structure of CheY-Mg(2+) in Complex with CheZ(C15) Peptide solved from a P1 Crystal Deposited 2007-04-20 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 2–129（128 aa）
Not recorded MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；37.5 % PEG 8000, 0.1 M ammonium thiocyanate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.69 Å R-free 0.285
2PMC Crystal Structure of CheY-Mg(2+) in Complex with CheZ(C15) Peptide solved from a P1 Crystal Deposited 2007-04-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 2–129（128 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；37.5 % PEG 8000, 0.1 M ammonium thiocyanate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.69 Å R-free 0.285
2PMC Crystal Structure of CheY-Mg(2+) in Complex with CheZ(C15) Peptide solved from a P1 Crystal Deposited 2007-04-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 2–129（128 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；37.5 % PEG 8000, 0.1 M ammonium thiocyanate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.69 Å R-free 0.285
8WIW Cryo-EM structure of the flagellar C ring in the CW state Deposited 2023-09-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 238 PDB declaration: 238-meric
Chain 4 1–129（129 aa）
Chain 9 1–129（129 aa）
Chain A5 1–129（129 aa）
Chain AN 1–129（129 aa）
Chain AT 1–129（129 aa）
Chain AZ 1–129（129 aa）
Chain Af 1–129（129 aa）
Chain Al 1–129（129 aa）
Chain Ar 1–129（129 aa）
Chain Ax 1–129（129 aa）
Chain B6 1–129（129 aa）
Chain BB 1–129（129 aa）
Chain BI 1–129（129 aa）
Chain BP 1–129（129 aa）
Chain BW 1–129（129 aa）
Chain Bd 1–129（129 aa）
Chain Bk 1–129（129 aa）
Chain Br 1–129（129 aa）
Chain By 1–129（129 aa）
Chain CC 1–129（129 aa）
Chain CJ 1–129（129 aa）
Chain CQ 1–129（129 aa）
Chain CX 1–129（129 aa）
Chain Ce 1–129（129 aa）
Chain Cl 1–129（129 aa）
Chain Cs 1–129（129 aa）
Chain Cz 1–129（129 aa）
Chain h 1–129（129 aa）
Chain i 1–129（129 aa）
Chain j 1–129（129 aa）
Chain k 1–129（129 aa）
Chain l 1–129（129 aa）
Chain m 1–129（129 aa）
Chain n 1–129（129 aa）
Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；Blot time: 4 Blot force: 10 Wait time: 30 Blot total: 1 Drain time: 2
Resolution 5.60 Å
8WOE Cryo-EM structure of the intact flagellar motor-hook complex in the CW state Deposited 2023-10-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 451 PDB declaration: 451-meric
Chain B6 1–129（129 aa）
Chain Bd 1–129（129 aa）
Chain Bk 1–129（129 aa）
Chain Br 1–129（129 aa）
Chain By 1–129（129 aa）
Chain C7 1–129（129 aa）
Chain CC 1–129（129 aa）
Chain CJ 1–129（129 aa）
Chain CQ 1–129（129 aa）
Chain CX 1–129（129 aa）
Chain Ce 1–129（129 aa）
Chain Cl 1–129（129 aa）
Chain Cs 1–129（129 aa）
Chain Cz 1–129（129 aa）
Chain D0 1–129（129 aa）
Chain D4 1–129（129 aa）
Chain DB 1–129（129 aa）
Chain DH 1–129（129 aa）
Chain DZ 1–129（129 aa）
Chain Df 1–129（129 aa）
Chain Dl 1–129（129 aa）
Chain Dr 1–129（129 aa）
Chain Dx 1–129（129 aa）
Chain E0 1–129（129 aa）
Chain E6 1–129（129 aa）
Chain E7 1–129（129 aa）
Chain E8 1–129（129 aa）
Chain E9 1–129（129 aa）
Chain ED 1–129（129 aa）
Chain EK 1–129（129 aa）
Chain ER 1–129（129 aa）
Chain EY 1–129（129 aa）
Chain FA 1–129（129 aa）
Chain FB 1–129（129 aa）
Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；Blot time: 4 Blot force: 10 Wait time: 30 Blot total: 1 Drain time: 2
Resolution 4.30 Å
8XP1 Cryo-EM structure of the protomers of the C ring in the CW state Deposited 2024-01-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain h 1–129（129 aa）
Chain i 1–129（129 aa）
Chain j 1–129（129 aa）
Mutation：D13K, Y106W Mutation：D13K, Y106W Mutation：D13K, Y106W No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；Blot time: 4 Blot force: 10 Wait time: 30 Blot total: 1 Drain time: 2
Resolution 4.40 Å

14 other PDB entries and 24 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CHEY_SALTY
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–128； UniProt 1–128

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 2che

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 2che
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2. Structure Basics 2. Structure Basics

Entry ID entry_id2che
Deposition date deposition_date1994-01-17
Structure title titleSTRUCTURE OF THE MG2+-BOUND FORM OF CHEY AND MECHANISM OF PHOSPHORYL TRANSFER IN BACTERIAL CHEMOTAXIS
Keywords keywordsSIGNAL TRANSDUCTION PROTEIN; SIGNAL TRANSDUCTION PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier14.91 Å
Radius of gyration Rg (electron density) rg_electron13.38 Å
Forward intensity I(0) i03769470.00
Molecular weight molecular_weight14015.0 kDa
Excluded volume excluded_volume17731 ų
Envelope volume envelope_volume19421 ų
Hydration-shell volume shell_volume12226 ų
Envelope diameter envelope_diameter42.7 Å
Shell Rg shell_rg19.28 Å
Envelope Rg envelope_rg13.56 Å
Shape Rg shape_rg13.37 Å
Total Rg total_rg14.68 Å
Total atoms total_atoms982
Residues n_residues128
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax44.7 Å
Rg (real space) rg_real14.76 Å
Rg uncertainty (real space) rg_real_error0.18 Å
I(0) (real space) i0_real3.7690e+06
I(0) uncertainty (real space) i0_real_error3.9360e+04
Rg (reciprocal space) rg_reciprocal14.78 Å
I(0) (reciprocal space) i0_reciprocal3769000.0000
Solution quality estimate total_estimate0.8954
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary44.2 Å
Skewness Skewness skewness-0.029
Kurtosis Kurtosis kurtosis-0.440
Angular range angular_range— – 0.5000 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha678200.0000
Real-space data points n_real_points80
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.897; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.970; Smooth: 0.977
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (3)

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7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

Domain ID domain_idd2chea_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.23 — Flavodoxin-like
Superfamily Superfamily superfamilyc.23.1 — CheY-like
Family Family familyc.23.1.1 — CheY-related

CATH v4.4 (1 domains)

Domain ID domain_id2cheA00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily2300 — Response regulator
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8. Citations (4)

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9. Files and Curves (10)