2hn2

Crystal structure of the CorA Mg2+ transporter homologue from T. maritima in complex with divalent cations

Method: X-RAY DIFFRACTION Dmax: 127.5 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

SAXS profile for 2hn2

P(r) 距离分布 P(r) Distribution

P(r) distribution for 2hn2
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id2hn2
沉积日期 deposition_date2006-07-11
结构标题 titleCrystal structure of the CorA Mg2+ transporter homologue from T. maritima in complex with divalent cations
关键词 keywordsintegral membrane protein; metal transporter protein; divalent cations, METAL TRANSPORT; METAL TRANSPORT
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier39.96
回转半径 Rg (电子) rg_electron39.79
零角强度 I(0) i0489908000.00
分子量 molecular_weight194300.0 kDa
排除体积 excluded_volume248600 ų
包络体积 envelope_volume325520 ų
水化壳体积 shell_volume67631 ų
包络直径 envelope_diameter124.6
壳层 Rg shell_rg46.41
包络 Rg envelope_rg39.17
形状 Rg shape_rg39.78
总 Rg total_rg40.21
总原子数 total_atoms13698
残基数 n_residues1655
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax127.5
Rg (实空间) rg_real39.81
Rg 误差 (实空间) rg_real_error0.89
I(0) (实空间) i0_real4.8990e+08
I(0) 误差 (实空间) i0_real_error8.1330e+06
Rg (倒空间) rg_reciprocal39.96
I(0) (倒空间) i0_reciprocal490000000.0000
解质量估计 total_estimate0.9002
解质量评级 solution_quality EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary54.2
偏度 Skewness skewness0.131
峰度 Kurtosis kurtosis-0.545
角度范围 angular_range— – 0.2000 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha226500000.0000
实空间数据点数 n_real_points41
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.915; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.996; Smooth: 0.959

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (2)

6. 折叠分类 (SCOP + CATH) 25 domains

SCOP 2.08 (10 domains)

结构域编号 domain_idd2hn2a1
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.328 — CorA soluble domain-like
超家族 Superfamily superfamilyd.328.1 — CorA soluble domain-like
家族 Family familyd.328.1.1 — CorA soluble domain-like
结构域编号 domain_idd2hn2a2
类 Class classf — Membrane and cell surface proteins and peptides
折叠类型 Fold foldf.17 — Transmembrane helix hairpin
超家族 Superfamily superfamilyf.17.3 — Magnesium transport protein CorA, transmembrane region
家族 Family familyf.17.3.1 — Magnesium transport protein CorA, transmembrane region
结构域编号 domain_idd2hn2b1
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.328 — CorA soluble domain-like
超家族 Superfamily superfamilyd.328.1 — CorA soluble domain-like
家族 Family familyd.328.1.1 — CorA soluble domain-like
结构域编号 domain_idd2hn2b2
类 Class classf — Membrane and cell surface proteins and peptides
折叠类型 Fold foldf.17 — Transmembrane helix hairpin
超家族 Superfamily superfamilyf.17.3 — Magnesium transport protein CorA, transmembrane region
家族 Family familyf.17.3.1 — Magnesium transport protein CorA, transmembrane region
结构域编号 domain_idd2hn2c1
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.328 — CorA soluble domain-like
超家族 Superfamily superfamilyd.328.1 — CorA soluble domain-like
家族 Family familyd.328.1.1 — CorA soluble domain-like
结构域编号 domain_idd2hn2c2
类 Class classf — Membrane and cell surface proteins and peptides
折叠类型 Fold foldf.17 — Transmembrane helix hairpin
超家族 Superfamily superfamilyf.17.3 — Magnesium transport protein CorA, transmembrane region
家族 Family familyf.17.3.1 — Magnesium transport protein CorA, transmembrane region
结构域编号 domain_idd2hn2d1
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.328 — CorA soluble domain-like
超家族 Superfamily superfamilyd.328.1 — CorA soluble domain-like
家族 Family familyd.328.1.1 — CorA soluble domain-like
结构域编号 domain_idd2hn2d2
类 Class classf — Membrane and cell surface proteins and peptides
折叠类型 Fold foldf.17 — Transmembrane helix hairpin
超家族 Superfamily superfamilyf.17.3 — Magnesium transport protein CorA, transmembrane region
家族 Family familyf.17.3.1 — Magnesium transport protein CorA, transmembrane region
结构域编号 domain_idd2hn2e1
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.328 — CorA soluble domain-like
超家族 Superfamily superfamilyd.328.1 — CorA soluble domain-like
家族 Family familyd.328.1.1 — CorA soluble domain-like
结构域编号 domain_idd2hn2e2
类 Class classf — Membrane and cell surface proteins and peptides
折叠类型 Fold foldf.17 — Transmembrane helix hairpin
超家族 Superfamily superfamilyf.17.3 — Magnesium transport protein CorA, transmembrane region
家族 Family familyf.17.3.1 — Magnesium transport protein CorA, transmembrane region

CATH v4.4 (15 domains)

结构域编号 domain_id2hn2A01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology460 — Beta Polymerase; domain 2
同源超家族 H-superfamily homologous superfamily20 — CorA soluble domain-like
结构域编号 domain_id2hn2A02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily340 — Magnesium transport protein CorA, transmembrane region
结构域编号 domain_id2hn2A03
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily340 — Magnesium transport protein CorA, transmembrane region
结构域编号 domain_id2hn2B01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology460 — Beta Polymerase; domain 2
同源超家族 H-superfamily homologous superfamily20 — CorA soluble domain-like
结构域编号 domain_id2hn2B02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily340 — Magnesium transport protein CorA, transmembrane region
结构域编号 domain_id2hn2B03
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily340 — Magnesium transport protein CorA, transmembrane region
结构域编号 domain_id2hn2C01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology460 — Beta Polymerase; domain 2
同源超家族 H-superfamily homologous superfamily20 — CorA soluble domain-like
结构域编号 domain_id2hn2C02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily340 — Magnesium transport protein CorA, transmembrane region
结构域编号 domain_id2hn2C03
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily340 — Magnesium transport protein CorA, transmembrane region
结构域编号 domain_id2hn2D01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology460 — Beta Polymerase; domain 2
同源超家族 H-superfamily homologous superfamily20 — CorA soluble domain-like
结构域编号 domain_id2hn2D02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily340 — Magnesium transport protein CorA, transmembrane region
结构域编号 domain_id2hn2D03
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily340 — Magnesium transport protein CorA, transmembrane region
结构域编号 domain_id2hn2E01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology460 — Beta Polymerase; domain 2
同源超家族 H-superfamily homologous superfamily20 — CorA soluble domain-like
结构域编号 domain_id2hn2E02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily340 — Magnesium transport protein CorA, transmembrane region
结构域编号 domain_id2hn2E03
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily340 — Magnesium transport protein CorA, transmembrane region

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