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3e86

High resolution Crystal Structure of the open NaK channel pore

Method: X-RAY DIFFRACTION Dmax: 107.4 Å Quality: REASONABLE
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Potassium channel protein

Bacillus cereus

UniProt Q81HW2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count Chain A； UniProt 19–110 Fragment：transmembrane domain, RESIDUES 19-110 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 CS CESIUM ION × 4 CA CALCIUM ION × 4 NA SODIUM ION × 8 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 9.5；293 K；100mM Glycine buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), 1mM CaCl2, pH 9.5, vapor diffusion, sitting drop, temperature 293K, VAPOR DIFFUSION, SITTING DROP Resolution 1.60 Å R-free 0.242
2 Protein homooligomer Homooligomer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count Chain B； UniProt 19–110 Fragment：transmembrane domain, RESIDUES 19-110 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 CS CESIUM ION × 4 NA SODIUM ION × 8 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 9.5；293 K；100mM Glycine buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), 1mM CaCl2, pH 9.5, vapor diffusion, sitting drop, temperature 293K, VAPOR DIFFUSION, SITTING DROP Resolution 1.60 Å R-free 0.242

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 3E86 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2Q67 Crystal Structure of Nak channel D66A mutant Deposited 2007-06-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–110（110 aa）
Chain B 1–110（110 aa）
Mutation：D66A Mutation：D66A NA SODIUM ION × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；200 mM CaCl2, 100 mM Tris-HCl, 37-42% PEG400, 4% t-Butanol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.30 Å R-free 0.266
2Q68 Crystal Structure of Nak channel D66A, S70E double mutants Deposited 2007-06-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–110（110 aa）
Chain B 1–110（110 aa）
Mutation：D66A, S70E Mutation：D66A, S70E CA CALCIUM ION × 4 NA SODIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；200 mM CaCl2, 100 mM Tris-HCl, 37-42% PEG400, 4% t-Butanol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.50 Å R-free 0.262
2Q69 Crystal Structure of Nak channel D66N mutant Deposited 2007-06-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–110（110 aa）
Chain B 1–110（110 aa）
Mutation：D66N Mutation：D66N NA SODIUM ION × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；200 mM CaCl2, 100 mM Tris-HCl, 37-42% PEG400, 4% t-Butanol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.40 Å R-free 0.267
2Q6A Crystal Structure of Nak channel D66E mutant Deposited 2007-06-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–110（110 aa）
Chain B 1–110（110 aa）
Mutation：D66E Mutation：D66E CA CALCIUM ION × 4 NA SODIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；200 mM CaCl2, 100 mM Tris-HCl, 37-42% PEG400, 4% t-Butanol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.60 Å R-free 0.280
3E83 Crystal Structure of the the open NaK channel pore Deposited 2008-08-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 19–110（92 aa） Fragment：transmembrane domain, residues 19-110
Not recorded CS CESIUM ION × 4 NA SODIUM ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), 500mM NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.80 Å R-free 0.236
3E83 Crystal Structure of the the open NaK channel pore Deposited 2008-08-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 19–110（92 aa） Fragment：transmembrane domain, residues 19-110
Not recorded CS CESIUM ION × 4 NA SODIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), 500mM NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.80 Å R-free 0.236
3E89 Crystal Structure of the the open NaK channel-low Na+ complex Deposited 2008-08-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 19–110（92 aa） Fragment：transmembrane domain, residues 19-110
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 CS CESIUM ION × 4 NA SODIUM ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.80 Å R-free 0.248
3E89 Crystal Structure of the the open NaK channel-low Na+ complex Deposited 2008-08-19 Different construct Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 19–110（92 aa） Fragment：transmembrane domain, residues 19-110
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 CS CESIUM ION × 4 NA SODIUM ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.80 Å R-free 0.248
3E8B Crystal Structure of the the open NaK channel- Rb+ complex Deposited 2008-08-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 19–110（92 aa） Fragment：transmembrane domain, residues 19-110
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 RB RUBIDIUM ION × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.70 Å R-free 0.229
3E8B Crystal Structure of the the open NaK channel- Rb+ complex Deposited 2008-08-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 19–110（92 aa） Fragment：transmembrane domain, residues 19-110
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 RB RUBIDIUM ION × 20 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.70 Å R-free 0.229
3E8F Crystal Structure of the the open NaK channel- K+/Ba2+ Deposited 2008-08-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 19–110（92 aa） Fragment：transmembrane domain, residues 19-110
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 BA BARIUM ION × 8 K POTASSIUM ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes Buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), Soaked in stabilization solution containing 67mM KCl and 33mM BaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.00 Å R-free 0.248
3E8F Crystal Structure of the the open NaK channel- K+/Ba2+ Deposited 2008-08-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 19–110（92 aa） Fragment：transmembrane domain, residues 19-110
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 BA BARIUM ION × 8 K POTASSIUM ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes Buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), Soaked in stabilization solution containing 67mM KCl and 33mM BaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.00 Å R-free 0.248
3E8G Crystal Structure of the the open NaK channel-Na+/Ca2+ complex Deposited 2008-08-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 19–110（92 aa） Fragment：transmembrane domain, residues 19-110
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 CS CESIUM ION × 4 CA CALCIUM ION × 4 NA SODIUM ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), 10mM CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.00 Å R-free 0.235
3E8G Crystal Structure of the the open NaK channel-Na+/Ca2+ complex Deposited 2008-08-19 Different construct Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 19–110（92 aa） Fragment：transmembrane domain, residues 19-110
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 CS CESIUM ION × 4 CA CALCIUM ION × 4 NA SODIUM ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), 10mM CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.00 Å R-free 0.235
3E8H Crystal Structure of the the open NaK channel-K+ complex Deposited 2008-08-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 19–110（92 aa） Fragment：transmembrane domain, residues 19-110
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 K POTASSIUM ION × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM GHepes buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.80 Å R-free 0.234
3E8H Crystal Structure of the the open NaK channel-K+ complex Deposited 2008-08-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 19–110（92 aa） Fragment：transmembrane domain, residues 19-110
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM GHepes buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.80 Å R-free 0.234
3K03 Crystal Structure of CNG mimicking NaK mutant, NaK-DTPP, K+ complex Deposited 2009-09-24 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 12 K POTASSIUM ION × 20 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；293 K；MPD, KCl, pH 6.5-8.5, vapor diffusion, temperature 293K
Resolution 1.62 Å R-free 0.222
3K03 Crystal Structure of CNG mimicking NaK mutant, NaK-DTPP, K+ complex Deposited 2009-09-24 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；293 K；MPD, KCl, pH 6.5-8.5, vapor diffusion, temperature 293K
Resolution 1.62 Å R-free 0.222
3K04 Crystal Structure of CNG mimicking NaK mutant, NaK-DTPP, Na+ complex Deposited 2009-09-24 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 16 NA SODIUM ION × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；293 K；MPD, NaCl, pH 6.5-8.5, vapor diffusion, temperature 293K
Resolution 1.58 Å R-free 0.235
3K04 Crystal Structure of CNG mimicking NaK mutant, NaK-DTPP, Na+ complex Deposited 2009-09-24 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 NA SODIUM ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；293 K；MPD, NaCl, pH 6.5-8.5, vapor diffusion, temperature 293K
Resolution 1.58 Å R-free 0.235
3K06 Crystal Structure of CNG mimicking NaK mutant, NaK-NTPP, K+ complex Deposited 2009-09-24 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 20 K POTASSIUM ION × 20 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；293 K；MPD, KCl, pH 6.5-8.5, vapor diffusion, temperature 293K
Resolution 1.58 Å R-free 0.214
3K06 Crystal Structure of CNG mimicking NaK mutant, NaK-NTPP, K+ complex Deposited 2009-09-24 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；293 K；MPD, KCl, pH 6.5-8.5, vapor diffusion, temperature 293K
Resolution 1.58 Å R-free 0.214
3K08 Crystal Structure of CNG mimicking NaK mutant, NaK-NTPP, Na+ complex Deposited 2009-09-24 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 16 NA SODIUM ION × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；293 K；MPD, NaCl, pH 6.5-8.5, vapor diffusion, temperature 293K
Resolution 1.62 Å R-free 0.227
3K08 Crystal Structure of CNG mimicking NaK mutant, NaK-NTPP, Na+ complex Deposited 2009-09-24 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 NA SODIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；293 K；MPD, NaCl, pH 6.5-8.5, vapor diffusion, temperature 293K
Resolution 1.62 Å R-free 0.227
3K0D Crystal Structure of CNG mimicking NaK mutant, NaK-ETPP, K+ complex Deposited 2009-09-24 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 12 K POTASSIUM ION × 20 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；293 K；MPD, KCl, pH 6.5-8.5, vapor diffusion, temperature 293K
Resolution 1.95 Å R-free 0.239
3K0D Crystal Structure of CNG mimicking NaK mutant, NaK-ETPP, K+ complex Deposited 2009-09-24 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；293 K；MPD, KCl, pH 6.5-8.5, vapor diffusion, temperature 293K
Resolution 1.95 Å R-free 0.239
3K0G Crystal Structure of CNG mimicking NaK mutant, NaK-ETPP, Na+ complex Deposited 2009-09-24 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 12 NA SODIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；293 K；MPD, NaCl, pH 6.5-8.5, vapor diffusion, temperature 293K
Resolution 1.95 Å R-free 0.228
3K0G Crystal Structure of CNG mimicking NaK mutant, NaK-ETPP, Na+ complex Deposited 2009-09-24 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 NA SODIUM ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；293 K；MPD, NaCl, pH 6.5-8.5, vapor diffusion, temperature 293K
Resolution 1.95 Å R-free 0.228
3T1C Crystal Structure of NaK Channel D66Y Mutant Deposited 2011-07-21 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa）
Mutation：D66Y K POTASSIUM ION × 20 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；100mM Tris, 70% MPD, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.80 Å R-free 0.233
3T1C Crystal Structure of NaK Channel D66Y Mutant Deposited 2011-07-21 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa）
Mutation：D66Y K POTASSIUM ION × 20 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；100mM Tris, 70% MPD, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.80 Å R-free 0.233
3T2M Crystal Structure of NaK Channel N68D Mutant Deposited 2011-07-22 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa）
Mutation：N68D K POTASSIUM ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；70% MPD, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.95 Å R-free 0.223
3T2M Crystal Structure of NaK Channel N68D Mutant Deposited 2011-07-22 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa）
Mutation：N68D K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；70% MPD, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.95 Å R-free 0.223
3T4D Crystal Structure of NaK2K Channel Y55F Mutant Deposited 2011-07-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa）
Mutation：Y55F, D66Y, N68D K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 9.5；293 K；65% MPD, 100mM Glycine, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.70 Å R-free 0.225
3T4D Crystal Structure of NaK2K Channel Y55F Mutant Deposited 2011-07-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa）
Mutation：Y55F, D66Y, N68D K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 9.5；293 K；65% MPD, 100mM Glycine, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.70 Å R-free 0.225
3T4Z Crystal Structure of NaK2K Channel Y55W Mutant Deposited 2011-07-26 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa）
Mutation：Y55W, D66Y, N68D K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 9；293 K；65% MPD, 100mM Glycine, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.90 Å R-free 0.241
3T4Z Crystal Structure of NaK2K Channel Y55W Mutant Deposited 2011-07-26 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa）
Mutation：Y55W, D66Y, N68D K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 9；293 K；65% MPD, 100mM Glycine, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.90 Å R-free 0.241
3TCU Crystal Structure of NaK2K Channel D68E Mutant Deposited 2011-08-09 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa）
Mutation：D66Y, N68E K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 9.5；293 K；60% MPD, 100mM Glycine, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.75 Å R-free 0.225
3TCU Crystal Structure of NaK2K Channel D68E Mutant Deposited 2011-08-09 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa）
Mutation：D66Y, N68E K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 9.5；293 K；60% MPD, 100mM Glycine, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.75 Å R-free 0.225
3TET Crystal Structure of NaK2K Channel Y66F Mutant Deposited 2011-08-15 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa）
Mutation：D66F, N68D K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；77.5% MPD, 100mM MES, 100mM KCl, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.90 Å R-free 0.239
3TET Crystal Structure of NaK2K Channel Y66F Mutant Deposited 2011-08-15 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa）
Mutation：D66F, N68D K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；77.5% MPD, 100mM MES, 100mM KCl, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.90 Å R-free 0.239
4PDL Structure of K+ selective NaK mutant in caesium Deposited 2014-04-19 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa） Fragment：UNP residues 20-110
Mutation：D66Y,N68D CS CESIUM ION × 12 NA SODIUM ION × 4 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 HEX HEXANE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；65% MPD, 100mM CsCl, 100mM MES, 4mM DM
Resolution 1.70 Å R-free 0.236
4PDL Structure of K+ selective NaK mutant in caesium Deposited 2014-04-19 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa） Fragment：UNP residues 20-110
Mutation：D66Y,N68D CS CESIUM ION × 8 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 HEX HEXANE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；65% MPD, 100mM CsCl, 100mM MES, 4mM DM
Resolution 1.70 Å R-free 0.236
4PDM Crystal Structure of K+ selective NaK mutant in rubidium Deposited 2014-04-19 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa） Fragment：UNP residues 20-110
Mutation：D66Y,N68D RB RUBIDIUM ION × 12 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；65%MPD, 100mM KCl, 100mM MES, 4mM n-Decyl-beta-D-Maltoside
Resolution 1.58 Å R-free 0.234
4PDM Crystal Structure of K+ selective NaK mutant in rubidium Deposited 2014-04-19 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa） Fragment：UNP residues 20-110
Mutation：D66Y,N68D RB RUBIDIUM ION × 12 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；65%MPD, 100mM KCl, 100mM MES, 4mM n-Decyl-beta-D-Maltoside
Resolution 1.58 Å R-free 0.234
4PDR Crystal Structure of a K+ selective NaK mutant in Barium and Sodium Deposited 2014-04-21 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa） Fragment：UNP residues 20-110
Mutation：D66Y,N68D BA BARIUM ION × 12 NA SODIUM ION × 4 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；65%MPD, 100mM KCl, 100mM MES, 4mM n-Decyl-beta-D-Maltoside
Resolution 1.85 Å R-free 0.227
4PDR Crystal Structure of a K+ selective NaK mutant in Barium and Sodium Deposited 2014-04-21 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa） Fragment：UNP residues 20-110
Mutation：D66Y,N68D BA BARIUM ION × 12 NA SODIUM ION × 4 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；65%MPD, 100mM KCl, 100mM MES, 4mM n-Decyl-beta-D-Maltoside
Resolution 1.85 Å R-free 0.227
4PDV Structure of K+ selective NaK mutant in barium and potassium Deposited 2014-04-22 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa） Fragment：UNP residues 20-110
Mutation：D66Y, N68D K POTASSIUM ION × 12 BA BARIUM ION × 8 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；65%MPD, 100mM KCl, 100mM MES, 4mM n-Decyl-beta-D-Maltoside
Resolution 1.82 Å R-free 0.232
4PDV Structure of K+ selective NaK mutant in barium and potassium Deposited 2014-04-22 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa） Fragment：UNP residues 20-110
Mutation：D66Y, N68D K POTASSIUM ION × 12 BA BARIUM ION × 4 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；65%MPD, 100mM KCl, 100mM MES, 4mM n-Decyl-beta-D-Maltoside
Resolution 1.82 Å R-free 0.232
4R50 Crystal Structure of CNG mimicking NaK-ETPP mutant cocrystallized with Li+ Deposited 2014-08-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 20–110（91 aa） Fragment：residues 20-110
Chain B 20–110（91 aa） Fragment：residues 20-110
Mutation：D66E, G67T, N68P, F69P Mutation：D66E, G67T, N68P, F69P MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 GLY GLYCINE × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；40-70% MPD, 20-100mM Glycine, pH 6.5 - 7.5, VAPOR DIFFUSION, HANGING DROP
Resolution 2.85 Å R-free 0.183
4R50 Crystal Structure of CNG mimicking NaK-ETPP mutant cocrystallized with Li+ Deposited 2014-08-20 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa） Fragment：residues 20-110
Mutation：D66E, G67T, N68P, F69P MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 GLY GLYCINE × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；40-70% MPD, 20-100mM Glycine, pH 6.5 - 7.5, VAPOR DIFFUSION, HANGING DROP
Resolution 2.85 Å R-free 0.183
4R50 Crystal Structure of CNG mimicking NaK-ETPP mutant cocrystallized with Li+ Deposited 2014-08-20 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa） Fragment：residues 20-110
Mutation：D66E, G67T, N68P, F69P GLY GLYCINE × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；40-70% MPD, 20-100mM Glycine, pH 6.5 - 7.5, VAPOR DIFFUSION, HANGING DROP
Resolution 2.85 Å R-free 0.183
4R6Z Crystal Structure of CNG mimicking NaK mutant, NaK-ETPP, Cs+ complex Deposited 2014-08-26 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa） Fragment：residues 20-110
Mutation：D66E, G67T, N68P, F69P CS CESIUM ION × 8 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 GLY GLYCINE × 20 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；CNG-ETPP(K+) crystals grown in 100mM MES pH 6.5, 60-66% (w/v) MPD, 100mM Glycine. Soaking overnight in 70% MPD, 10mM DM, 100mM Hepes pH 7.5 and 100mM CsCl, VAPOR DIFFUSION, HANGING DROP
Resolution 2.30 Å R-free 0.182
4R6Z Crystal Structure of CNG mimicking NaK mutant, NaK-ETPP, Cs+ complex Deposited 2014-08-26 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa） Fragment：residues 20-110
Mutation：D66E, G67T, N68P, F69P CS CESIUM ION × 12 GLY GLYCINE × 28 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；CNG-ETPP(K+) crystals grown in 100mM MES pH 6.5, 60-66% (w/v) MPD, 100mM Glycine. Soaking overnight in 70% MPD, 10mM DM, 100mM Hepes pH 7.5 and 100mM CsCl, VAPOR DIFFUSION, HANGING DROP
Resolution 2.30 Å R-free 0.182
4R7C Crystal Structure of CNG mimicking NaK-ETPP mutant cocrystallized with DiMethylammonium Deposited 2014-08-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa） Fragment：residues 20-110
Chain B 20–110（91 aa） Fragment：residues 20-110
Mutation：D66E, G67T, N68P, F69P Mutation：D66E, G67T, N68P, F69P GLY GLYCINE × 32 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 DMN DIMETHYLAMINE × 32 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；CNG-ETPP(DiMA+) cocrystals grown in 40-44% MPD, 100mM MES pH6.5 and 20-25 mM Glycine, VAPOR DIFFUSION, HANGING DROP
Resolution 2.30 Å R-free 0.248
4R7C Crystal Structure of CNG mimicking NaK-ETPP mutant cocrystallized with DiMethylammonium Deposited 2014-08-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain C 20–110（91 aa） Fragment：residues 20-110
Chain D 20–110（91 aa） Fragment：residues 20-110
Mutation：D66E, G67T, N68P, F69P Mutation：D66E, G67T, N68P, F69P GLY GLYCINE × 26 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 DMN DIMETHYLAMINE × 18 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；CNG-ETPP(DiMA+) cocrystals grown in 40-44% MPD, 100mM MES pH6.5 and 20-25 mM Glycine, VAPOR DIFFUSION, HANGING DROP
Resolution 2.30 Å R-free 0.248
4R8C Crystal Structure of CNG mimicking NaK-ETPP mutant in complex with Rb+ Deposited 2014-09-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa） Fragment：residues 20-110
Mutation：D66E, G67T, N68P, F69P RB RUBIDIUM ION × 12 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 GLY GLYCINE × 20 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；CNG-ETPP(K+) crystals grown in 100mM MES pH 6.5, 60-66% (w/v) MPD, 100mM Glycine, Soaking O.N. in 70% MPD, 10mM DM, 100mM Hepes pH 7.5 and 100mM RbCl, VAPOR DIFFUSION, HANGING DROP
Resolution 2.50 Å R-free 0.257
4R8C Crystal Structure of CNG mimicking NaK-ETPP mutant in complex with Rb+ Deposited 2014-09-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa） Fragment：residues 20-110
Mutation：D66E, G67T, N68P, F69P RB RUBIDIUM ION × 12 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 GLY GLYCINE × 20 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；CNG-ETPP(K+) crystals grown in 100mM MES pH 6.5, 60-66% (w/v) MPD, 100mM Glycine, Soaking O.N. in 70% MPD, 10mM DM, 100mM Hepes pH 7.5 and 100mM RbCl, VAPOR DIFFUSION, HANGING DROP
Resolution 2.50 Å R-free 0.257
4RAI Crystal Structure of CNG mimicking NaK-ETPP mutant in complex with Na+ Deposited 2014-09-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa） Fragment：residues 20-110
Mutation：D66E, G67T, N68P, F69P NA SODIUM ION × 16 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 GLY GLYCINE × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；CNG-ETPP(K+) crystals grown in 100mM MES pH 6.5, 60-66% (w/v) MPD, 100mM Glycine, Soaking O.N. in 70% MPD, 10mM DM, 100mM Hepes pH 7.5 and 100mM NaCl, VAPOR DIFFUSION, HANGING DROP
Resolution 2.31 Å R-free 0.215
4RAI Crystal Structure of CNG mimicking NaK-ETPP mutant in complex with Na+ Deposited 2014-09-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa） Fragment：residues 20-110
Mutation：D66E, G67T, N68P, F69P NA SODIUM ION × 12 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 12 GLY GLYCINE × 28 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；CNG-ETPP(K+) crystals grown in 100mM MES pH 6.5, 60-66% (w/v) MPD, 100mM Glycine, Soaking O.N. in 70% MPD, 10mM DM, 100mM Hepes pH 7.5 and 100mM NaCl, VAPOR DIFFUSION, HANGING DROP
Resolution 2.31 Å R-free 0.215
4RO2 Crystal Structure of CNG mimicking NaK-ETPP mutant cocrystallized with Methylammonium Deposited 2014-10-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa） Fragment：residues 20-110
Chain B 20–110（91 aa） Fragment：residues 20-110
Mutation：D66E, G67T, N68P, F69P Mutation：D66E, G67T, N68P, F69P 3P8 methylammonium ion × 2 GLY GLYCINE × 34 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；100mM MES pH 6.5, 25mM Glycine, 40-44% MPD, 100mM MACl, VAPOR DIFFUSION, HANGING DROP
Resolution 2.70 Å R-free 0.283
4RO2 Crystal Structure of CNG mimicking NaK-ETPP mutant cocrystallized with Methylammonium Deposited 2014-10-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain C 20–110（91 aa） Fragment：residues 20-110
Chain D 20–110（91 aa） Fragment：residues 20-110
Mutation：D66E, G67T, N68P, F69P Mutation：D66E, G67T, N68P, F69P 3P8 methylammonium ion × 2 GLY GLYCINE × 22 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；100mM MES pH 6.5, 25mM Glycine, 40-44% MPD, 100mM MACl, VAPOR DIFFUSION, HANGING DROP
Resolution 2.70 Å R-free 0.283
4ZBM Crystal structure of Drosophila cyclic nucleotide gated channel pore mimicking NaK mutant Deposited 2015-04-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–110（91 aa） Fragment：UNP residues 22-110
Not recorded K POTASSIUM ION × 12 BA BARIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；293 K；65%MPD, 100mM KCl, 100mM MES, 4mM n-Decyl-beta-D-Maltoside
Resolution 1.90 Å R-free 0.236
4ZBM Crystal structure of Drosophila cyclic nucleotide gated channel pore mimicking NaK mutant Deposited 2015-04-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 20–110（91 aa） Fragment：UNP residues 22-110
Not recorded K POTASSIUM ION × 8 BA BARIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；293 K；65%MPD, 100mM KCl, 100mM MES, 4mM n-Decyl-beta-D-Maltoside
Resolution 1.90 Å R-free 0.236
6FIZ Crystal Structure of CNG mimicking NaK-EAPP mutant (T67A) cocrystallized with K+ Deposited 2018-01-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 20–64（45 aa）
Chain A 72–110（39 aa）
Chain B 20–64（45 aa）
Chain B 72–110（39 aa）
Chain C 20–64（45 aa）
Chain C 72–110（39 aa）
Chain D 20–64（45 aa）
Chain D 72–110（39 aa）
Not recorded GLY GLYCINE × 10 K POTASSIUM ION × 3 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；40-44% MPD 100 mM MES pH 6.5 25mM Glycine
Resolution 2.63 Å R-free 0.269
6FIZ Crystal Structure of CNG mimicking NaK-EAPP mutant (T67A) cocrystallized with K+ Deposited 2018-01-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain E 20–64（45 aa）
Chain E 72–110（39 aa）
Chain F 20–64（45 aa）
Chain F 72–110（39 aa）
Chain G 20–64（45 aa）
Chain G 72–110（39 aa）
Chain H 20–64（45 aa）
Chain H 72–110（39 aa）
Not recorded GLY GLYCINE × 12 K POTASSIUM ION × 3 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；40-44% MPD 100 mM MES pH 6.5 25mM Glycine
Resolution 2.63 Å R-free 0.269
6FIZ Crystal Structure of CNG mimicking NaK-EAPP mutant (T67A) cocrystallized with K+ Deposited 2018-01-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain I 20–64（45 aa）
Chain I 72–110（39 aa）
Chain J 20–64（45 aa）
Chain J 72–110（39 aa）
Chain K 20–64（45 aa）
Chain K 72–110（39 aa）
Chain L 20–64（45 aa）
Chain L 72–110（39 aa）
Not recorded GLY GLYCINE × 17 K POTASSIUM ION × 3 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；40-44% MPD 100 mM MES pH 6.5 25mM Glycine
Resolution 2.63 Å R-free 0.269
6FIZ Crystal Structure of CNG mimicking NaK-EAPP mutant (T67A) cocrystallized with K+ Deposited 2018-01-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain M 20–64（45 aa）
Chain M 72–110（39 aa）
Chain N 20–64（45 aa）
Chain N 72–110（39 aa）
Chain O 20–64（45 aa）
Chain O 72–110（39 aa）
Chain P 20–64（45 aa）
Chain P 72–110（39 aa）
Not recorded GLY GLYCINE × 21 K POTASSIUM ION × 3 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；40-44% MPD 100 mM MES pH 6.5 25mM Glycine
Resolution 2.63 Å R-free 0.269
7OOR NaK C-DI mutant with Na+ and K+ Deposited 2021-05-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 19–110（92 aa）
Chain B 19–110（92 aa）
Mutation：D66C G67- N68D F69I Mutation：D66C G67- N68D F69I K POTASSIUM ION × 4 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 34 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 4 NA SODIUM ION × 10 CL CHLORIDE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；200mM KF, 40% MPD (2-Methyl-2,4-pentanediol racemate)
Resolution 1.47 Å R-free 0.183
7OOU NaK C-DI mutant with Li+ and K+ Deposited 2021-05-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 19–110（92 aa）
Chain B 19–110（92 aa）
Mutation：D66C G67- N68D F69I Mutation：D66C G67- N68D F69I K POTASSIUM ION × 8 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 20 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 4 ACT ACETATE ION × 20 CL CHLORIDE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；200mM K acetate, 40% MPD (2-Methyl-2,4-pentanediol racemate)
Resolution 1.80 Å R-free 0.206
7OPH NaK S-DI mutant with Na+ and K+ Deposited 2021-05-31 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 19–110（92 aa）
Chain B 19–110（92 aa）
Mutation：D66S G67- N68D F69I Mutation：D66S G67- N68D F69I K POTASSIUM ION × 12 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 22 CL CHLORIDE ION × 4 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；200 mM KCl, 100 mM HEPES (KOH) pH 7.5, 62% MPD (2-Methyl-2,4-pentanediol racemate)
Resolution 1.42 Å R-free 0.179
7OQ1 NaK S-ELM mutant with Na+ and K+ Deposited 2021-06-02 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 19–110（92 aa）
Chain B 19–110（92 aa）
Mutation：D66S G67- N68E F69L S70M Mutation：D66S G67- N68E F69L S70M MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 K POTASSIUM ION × 6 ACT ACETATE ION × 24 CL CHLORIDE ION × 2 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 2 NA SODIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100 mM HEPES (NaOH) pH 7.5, 47% MPD (2-Methyl-2,4-pentanediol racemate)
Resolution 1.85 Å R-free 0.186
7OQ2 NaK S-DI mutant soaked in Na+ Deposited 2021-06-02 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 19–110（92 aa）
Chain B 19–110（92 aa）
Mutation：D66S G67- N68D F69I Mutation：D66S G67- N68D F69I MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 36 NA SODIUM ION × 8 K POTASSIUM ION × 4 CL CHLORIDE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；200 mM KCl, 100 mM HEPES (KOH) pH 7.5, 62% MPD (2-Methyl-2,4-pentanediol racemate) - soaked in 100 mM NaCl, 100 mM Tris-HCl pH 8.0, 50% MPD
Resolution 1.70 Å R-free 0.233
7PA0 NaK C-DI F92A mutant with Rb+ and K+ Deposited 2021-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 19–110（92 aa）
Chain B 19–110（92 aa）
Mutation：D66C G67- N68D F69I F92A Mutation：D66C G67- N68D F69I F92A RB RUBIDIUM ION × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 36 K POTASSIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；200 mM RbCl, 100 mM Tris-HCl pH 8.0, 63% MPD (2-Methyl-2,4-pentanediol racemate)
Resolution 1.95 Å R-free 0.248
8A35 NaK C-DI mutant with Rb+ and Na+ Deposited 2022-06-07 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 19–110（92 aa）
Mutation：D66C G67- N68D F69I RB RUBIDIUM ION × 8 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 NA SODIUM ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100 mM HEPES (NaOH) pH 7.5, 5% PEG 4000, 30% MPD (2-Methyl-2,4-pentanediol racemate)
Resolution 2.05 Å R-free 0.217
8A35 NaK C-DI mutant with Rb+ and Na+ Deposited 2022-06-07 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 19–110（92 aa）
Mutation：D66C G67- N68D F69I RB RUBIDIUM ION × 8 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 20 NA SODIUM ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100 mM HEPES (NaOH) pH 7.5, 5% PEG 4000, 30% MPD (2-Methyl-2,4-pentanediol racemate)
Resolution 2.05 Å R-free 0.217
8A7X NaK C-DI F92A mutant soaked in Cs+ Deposited 2022-06-21 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 19–110（92 aa）
Mutation：D66C G67- N68D F69I F92A CS CESIUM ION × 4 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 28 K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；200 mM CsCl 100 mM Tris-HCl pH 8.0, 63% MPD (2-Methyl-2,4-pentanediol racemate) - soaked in 90 mM CsCl, 100 mM Kryptofix-222, 50% MPD
Resolution 2.10 Å R-free 0.240
8A7X NaK C-DI F92A mutant soaked in Cs+ Deposited 2022-06-21 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 19–110（92 aa）
Mutation：D66C G67- N68D F69I F92A CS CESIUM ION × 12 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 44 K POTASSIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；200 mM CsCl 100 mM Tris-HCl pH 8.0, 63% MPD (2-Methyl-2,4-pentanediol racemate) - soaked in 90 mM CsCl, 100 mM Kryptofix-222, 50% MPD
Resolution 2.10 Å R-free 0.240
8AYP NaK C-DI mutant with Rb+ and Ba2+ Deposited 2022-09-02 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 19–110（92 aa）
Chain B 19–110（92 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 24 RB RUBIDIUM ION × 4 BA BARIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；100 mM MES (NaOH), 40% v/v MPD (2-Methyl-2,4-pentanediol racemate)
Resolution 2.10 Å R-free 0.267
8AYP NaK C-DI mutant with Rb+ and Ba2+ Deposited 2022-09-02 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain C 19–110（92 aa）
Chain D 19–110（92 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 34 RB RUBIDIUM ION × 4 BA BARIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；100 mM MES (NaOH), 40% v/v MPD (2-Methyl-2,4-pentanediol racemate)
Resolution 2.10 Å R-free 0.267
8AYQ NaK C-DI mutant with Rb+ and Ca2+ Deposited 2022-09-02 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 19–110（92 aa）
Chain B 19–110（92 aa）
Not recorded RB RUBIDIUM ION × 4 CA CALCIUM ION × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 22 CL CHLORIDE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；100 mM MES (NaOH), 40% v/v MPD (2-Methyl-2,4-pentanediol racemate)
Resolution 2.75 Å R-free 0.300
8AYQ NaK C-DI mutant with Rb+ and Ca2+ Deposited 2022-09-02 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain C 19–110（92 aa）
Chain D 19–110（92 aa）
Not recorded RB RUBIDIUM ION × 4 CA CALCIUM ION × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；100 mM MES (NaOH), 40% v/v MPD (2-Methyl-2,4-pentanediol racemate)
Resolution 2.75 Å R-free 0.300

44 other PDB entries and 81 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q81HW2_BACCR
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–92； UniProt 19–110 Author chain B； PDBConstruct 1–92； UniProt 19–110

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 3e86

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 3e86
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2. Structure Basics 2. Structure Basics

Entry ID entry_id3e86
Deposition date deposition_date2008-08-19
Structure title titleHigh resolution Crystal Structure of the open NaK channel pore
Keywords keywordsnon-selective cation channel, membrane protein, tetrameric cation channel family, 2-transmembrane helix channels, Ionic channel; MEMBRANE PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier27.96 Å
Radius of gyration Rg (electron density) rg_electron28.77 Å
Forward intensity I(0) i05449580.00
Molecular weight molecular_weight21185.0 kDa
Excluded volume excluded_volume27955 ų
Envelope volume envelope_volume37770 ų
Hydration-shell volume shell_volume12902 ų
Envelope diameter envelope_diameter113.6 Å
Shell Rg shell_rg30.24 Å
Envelope Rg envelope_rg29.42 Å
Shape Rg shape_rg28.77 Å
Total Rg total_rg28.99 Å
Total atoms total_atoms1484
Residues n_residues187
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax107.4 Å
Rg (real space) rg_real28.82 Å
Rg uncertainty (real space) rg_real_error1.44 Å
I(0) (real space) i0_real5.4500e+06
I(0) uncertainty (real space) i0_real_error8.8350e+04
Rg (reciprocal space) rg_reciprocal28.55 Å
I(0) (reciprocal space) i0_reciprocal5449000.0000
Solution quality estimate total_estimate0.6868
Solution quality rating solution_quality REASONABLE a REASONABLE solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary18.8 Å
Skewness Skewness skewness0.700
Kurtosis Kurtosis kurtosis-0.165
Angular range angular_range— – 0.2850 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha277100.0000
Real-space data points n_real_points58
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.307; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.049; Smooth: 0.952
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (6)

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7. Fold Classification (SCOP + CATH) 2 domains

CATH v4.4 (2 domains)

Domain ID domain_id3e86A00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology287 — Helix Hairpins
Homologous superfamily homologous superfamily70 —
Domain ID domain_id3e86B00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology287 — Helix Hairpins
Homologous superfamily homologous superfamily70 —
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8. Citations (1)

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9. Files and Curves (10)