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3fhp

A neutron crystallographic analysis of a porcine 2Zn insulin at 2.0 A resolution

Method: NEUTRON DIFFRACTION Dmax: 53.9 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Insulin

Sus scrofa

UniProt P01315

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 12 PDB declaration: dodecameric（12） Consistent with protein copy count Chain A； UniProt 88–108 Chain B； UniProt 25–54 Chain C； UniProt 88–108 Chain D； UniProt 25–54 Fragment：UNP residues 88-108 Fragment：UNP residues 25-54 ZN ZINC ION × 6 NEUTRON DIFFRACTION X-ray crystallization conditions：LIQUID DIFFUSION；pH 6.3；293 K；2.3mg/ml insulin, 50mM sodium citrate, 6mM zinc acetate, 17% acetone, pH6.3, LIQUID DIFFUSION, temperature 293K Resolution 2.00 Å R-free 0.247

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 3FHP Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1B17 PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 5.00 COORDINATES) Deposited 1998-11-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5；pH 5.00
Resolution 1.70 Å R-free 0.219
1B17 PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 5.00 COORDINATES) Deposited 1998-11-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5；pH 5.00
Resolution 1.70 Å R-free 0.219
1B17 PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 5.00 COORDINATES) Deposited 1998-11-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5；pH 5.00
Resolution 1.70 Å R-free 0.219
1B18 PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 5.53 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.53；pH 5.53
Resolution 1.80 Å R-free 0.216
1B18 PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 5.53 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.53；pH 5.53
Resolution 1.80 Å R-free 0.216
1B18 PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 5.53 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.53；pH 5.53
Resolution 1.80 Å R-free 0.216
1B19 PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 5.80 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.8；pH 5.80
Resolution 1.80 Å R-free 0.231
1B19 PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 5.80 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.8；pH 5.80
Resolution 1.80 Å R-free 0.231
1B19 PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 5.80 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.8；pH 5.80
Resolution 1.80 Å R-free 0.231
1B2A PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.00 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6；pH 6.00
Resolution 1.70 Å R-free 0.230
1B2A PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.00 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6；pH 6.00
Resolution 1.70 Å R-free 0.230
1B2A PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.00 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6；pH 6.00
Resolution 1.70 Å R-free 0.230
1B2B PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.16 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.16；pH 6.16
Resolution 1.80 Å R-free 0.231
1B2B PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.16 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.16；pH 6.16
Resolution 1.80 Å R-free 0.231
1B2B PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.16 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.16；pH 6.16
Resolution 1.80 Å R-free 0.231
1B2C PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.26 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.26；pH 6.26
Resolution 1.80 Å R-free 0.223
1B2C PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.26 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.26；pH 6.26
Resolution 1.80 Å R-free 0.223
1B2D PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.35 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.35；pH 6.35
Resolution 1.70 Å R-free 0.229
1B2D PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.35 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.35；pH 6.35
Resolution 1.70 Å R-free 0.229
1B2D PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.35 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.35；pH 6.35
Resolution 1.70 Å R-free 0.229
1B2E PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.50 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；pH 6.50
Resolution 1.90 Å R-free 0.229
1B2E PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.50 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；pH 6.50
Resolution 1.90 Å R-free 0.229
1B2E PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.50 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；pH 6.50
Resolution 1.90 Å R-free 0.229
1B2F PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.98 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.98；pH 6.98
Resolution 1.90 Å R-free 0.244
1B2F PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.98 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.98；pH 6.98
Resolution 1.90 Å R-free 0.244
1B2G PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 9.00 COORDINATES) Deposited 1998-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 9；pH 9.00
Resolution 1.80 Å R-free 0.226
1DEI DESHEPTAPEPTIDE (B24-B30) INSULIN Deposited 1996-05-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–47（23 aa）
Mutation：CHAIN B, DEL(24-30) Mutation：CHAIN B, DEL(24-30) No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.60 Å R-free 0.215
1DEI DESHEPTAPEPTIDE (B24-B30) INSULIN Deposited 1996-05-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 88–108（21 aa）
Chain D 25–47（23 aa）
Mutation：CHAIN B, DEL(24-30) Mutation：CHAIN B, DEL(24-30) No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.60 Å R-free 0.215
1DEI DESHEPTAPEPTIDE (B24-B30) INSULIN Deposited 1996-05-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–47（23 aa）
Chain C 88–108（21 aa）
Chain D 25–47（23 aa）
Mutation：CHAIN B, DEL(24-30) Mutation：CHAIN B, DEL(24-30) Mutation：CHAIN B, DEL(24-30) Mutation：CHAIN B, DEL(24-30) No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.60 Å R-free 0.215
1IZA ROLE OF B13 GLU IN INSULIN ASSEMBLY: THE HEXAMER STRUCTURE OF RECOMBINANT MUTANT (B13 GLU-> GLN) INSULIN Deposited 1992-10-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–53（29 aa）
Mutation：E13Q No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å
1IZA ROLE OF B13 GLU IN INSULIN ASSEMBLY: THE HEXAMER STRUCTURE OF RECOMBINANT MUTANT (B13 GLU-> GLN) INSULIN Deposited 1992-10-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 88–108（21 aa）
Chain D 25–53（29 aa）
Mutation：E13Q No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å
1IZA ROLE OF B13 GLU IN INSULIN ASSEMBLY: THE HEXAMER STRUCTURE OF RECOMBINANT MUTANT (B13 GLU-> GLN) INSULIN Deposited 1992-10-16 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric
Chain A 88–108（21 aa）
Chain B 25–53（29 aa）
Chain C 88–108（21 aa）
Chain D 25–53（29 aa）
Mutation：E13Q Mutation：E13Q No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å
1IZA ROLE OF B13 GLU IN INSULIN ASSEMBLY: THE HEXAMER STRUCTURE OF RECOMBINANT MUTANT (B13 GLU-> GLN) INSULIN Deposited 1992-10-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–53（29 aa）
Chain C 88–108（21 aa）
Chain D 25–53（29 aa）
Mutation：E13Q Mutation：E13Q No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å
1IZB ROLE OF B13 GLU IN INSULIN ASSEMBLY: THE HEXAMER STRUCTURE OF RECOMBINANT MUTANT (B13 GLU-> GLN) INSULIN Deposited 1992-10-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–53（29 aa）
Mutation：E13Q ZN ZINC ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
1IZB ROLE OF B13 GLU IN INSULIN ASSEMBLY: THE HEXAMER STRUCTURE OF RECOMBINANT MUTANT (B13 GLU-> GLN) INSULIN Deposited 1992-10-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 88–108（21 aa）
Chain D 25–53（29 aa）
Mutation：E13Q ZN ZINC ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
1IZB ROLE OF B13 GLU IN INSULIN ASSEMBLY: THE HEXAMER STRUCTURE OF RECOMBINANT MUTANT (B13 GLU-> GLN) INSULIN Deposited 1992-10-16 Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric
Chain A 88–108（21 aa）
Chain B 25–53（29 aa）
Chain C 88–108（21 aa）
Chain D 25–53（29 aa）
Mutation：E13Q Mutation：E13Q ZN ZINC ION × 6 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
1IZB ROLE OF B13 GLU IN INSULIN ASSEMBLY: THE HEXAMER STRUCTURE OF RECOMBINANT MUTANT (B13 GLU-> GLN) INSULIN Deposited 1992-10-16 Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric
Chain A 88–108（21 aa）
Chain B 25–53（29 aa）
Chain C 88–108（21 aa）
Chain D 25–53（29 aa）
Mutation：E13Q Mutation：E13Q ZN ZINC ION × 6 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
1IZB ROLE OF B13 GLU IN INSULIN ASSEMBLY: THE HEXAMER STRUCTURE OF RECOMBINANT MUTANT (B13 GLU-> GLN) INSULIN Deposited 1992-10-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 88–108（21 aa）
Chain B 25–53（29 aa）
Mutation：E13Q ZN ZINC ION × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
1IZB ROLE OF B13 GLU IN INSULIN ASSEMBLY: THE HEXAMER STRUCTURE OF RECOMBINANT MUTANT (B13 GLU-> GLN) INSULIN Deposited 1992-10-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain C 88–108（21 aa）
Chain D 25–53（29 aa）
Mutation：E13Q ZN ZINC ION × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
1IZB ROLE OF B13 GLU IN INSULIN ASSEMBLY: THE HEXAMER STRUCTURE OF RECOMBINANT MUTANT (B13 GLU-> GLN) INSULIN Deposited 1992-10-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 7 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–53（29 aa）
Chain C 88–108（21 aa）
Chain D 25–53（29 aa）
Mutation：E13Q Mutation：E13Q ZN ZINC ION × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
1IZB ROLE OF B13 GLU IN INSULIN ASSEMBLY: THE HEXAMER STRUCTURE OF RECOMBINANT MUTANT (B13 GLU-> GLN) INSULIN Deposited 1992-10-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 8 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–53（29 aa）
Chain C 88–108（21 aa）
Chain D 25–53（29 aa）
Mutation：E13Q Mutation：E13Q ZN ZINC ION × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
1M5A Crystal Structure of 2-Co(2+)-Insulin at 1.2A Resolution Deposited 2002-07-09 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded CO COBALT (II) ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions SLOW COOLING；pH 6.3；300 K；cobalt acetate, trisodium citrate, acetone, hydrochloric acid, pH 6.3, SLOW COOLING, temperature 300K
Resolution 1.20 Å R-free 0.241
1MPJ X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL Deposited 1995-09-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded IPH PHENOL × 1 ZN ZINC ION × 1 CL CHLORIDE ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.30 Å
1MPJ X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL Deposited 1995-09-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 1 CL CHLORIDE ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.30 Å
1MPJ X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL Deposited 1995-09-13 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded IPH PHENOL × 3 ZN ZINC ION × 6 CL CHLORIDE ION × 6 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.30 Å
1MPJ X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL Deposited 1995-09-13 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded IPH PHENOL × 1 ZN ZINC ION × 2 CL CHLORIDE ION × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.30 Å
1MPJ X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL Deposited 1995-09-13 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded IPH PHENOL × 1 ZN ZINC ION × 2 CL CHLORIDE ION × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.30 Å
1MPJ X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL Deposited 1995-09-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded IPH PHENOL × 3 ZN ZINC ION × 3 CL CHLORIDE ION × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.30 Å
1MPJ X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL Deposited 1995-09-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 7 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 3 CL CHLORIDE ION × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.30 Å
1SDB PORCINE DESB1-2 DESPENTAPEPTIDE(B26-B30) INSULIN Deposited 1996-01-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 27–49（23 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.65 Å R-free 0.220
1WAV CRYSTAL STRUCTURE OF FORM B MONOCLINIC CRYSTAL OF INSULIN Deposited 1996-02-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded IPH PHENOL × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å
1WAV CRYSTAL STRUCTURE OF FORM B MONOCLINIC CRYSTAL OF INSULIN Deposited 1996-02-28 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 10 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded IPH PHENOL × 2 ZN ZINC ION × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å
1WAV CRYSTAL STRUCTURE OF FORM B MONOCLINIC CRYSTAL OF INSULIN Deposited 1996-02-28 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 11 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain E 88–108（21 aa）
Chain F 25–54（30 aa）
Chain G 88–108（21 aa）
Chain H 25–54（30 aa）
Not recorded IPH PHENOL × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å
1WAV CRYSTAL STRUCTURE OF FORM B MONOCLINIC CRYSTAL OF INSULIN Deposited 1996-02-28 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 12 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain I 88–108（21 aa）
Chain J 25–54（30 aa）
Chain K 88–108（21 aa）
Chain L 25–54（30 aa）
Not recorded IPH PHENOL × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å
1WAV CRYSTAL STRUCTURE OF FORM B MONOCLINIC CRYSTAL OF INSULIN Deposited 1996-02-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded IPH PHENOL × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å
1WAV CRYSTAL STRUCTURE OF FORM B MONOCLINIC CRYSTAL OF INSULIN Deposited 1996-02-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain E 88–108（21 aa）
Chain F 25–54（30 aa）
Not recorded IPH PHENOL × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å
1WAV CRYSTAL STRUCTURE OF FORM B MONOCLINIC CRYSTAL OF INSULIN Deposited 1996-02-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain G 88–108（21 aa）
Chain H 25–54（30 aa）
Not recorded IPH PHENOL × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å
1WAV CRYSTAL STRUCTURE OF FORM B MONOCLINIC CRYSTAL OF INSULIN Deposited 1996-02-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain I 88–108（21 aa）
Chain J 25–54（30 aa）
Not recorded IPH PHENOL × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å
1WAV CRYSTAL STRUCTURE OF FORM B MONOCLINIC CRYSTAL OF INSULIN Deposited 1996-02-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain K 88–108（21 aa）
Chain L 25–54（30 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å
1WAV CRYSTAL STRUCTURE OF FORM B MONOCLINIC CRYSTAL OF INSULIN Deposited 1996-02-28 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 7 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Chain E 88–108（21 aa）
Chain F 25–54（30 aa）
Chain G 88–108（21 aa）
Chain H 25–54（30 aa）
Chain I 88–108（21 aa）
Chain J 25–54（30 aa）
Chain K 88–108（21 aa）
Chain L 25–54（30 aa）
Not recorded IPH PHENOL × 6 ZN ZINC ION × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å
1WAV CRYSTAL STRUCTURE OF FORM B MONOCLINIC CRYSTAL OF INSULIN Deposited 1996-02-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 8 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain E 88–108（21 aa）
Chain F 25–54（30 aa）
Chain I 88–108（21 aa）
Chain J 25–54（30 aa）
Not recorded IPH PHENOL × 3 ZN ZINC ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å
1WAV CRYSTAL STRUCTURE OF FORM B MONOCLINIC CRYSTAL OF INSULIN Deposited 1996-02-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 9 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Chain G 88–108（21 aa）
Chain H 25–54（30 aa）
Chain K 88–108（21 aa）
Chain L 25–54（30 aa）
Not recorded IPH PHENOL × 3 ZN ZINC ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å
1ZEI CROSS-LINKED B28 ASP INSULIN Deposited 1998-07-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 1–51（51 aa）
Chain B 1–51（51 aa）
Chain C 1–51（51 aa）
Chain D 1–51（51 aa）
Chain E 1–51（51 aa）
Chain F 1–51（51 aa）
Mutation：P28D Mutation：P28D Mutation：P28D Mutation：P28D Mutation：P28D Mutation：P28D CRS M-CRESOL × 8 ZN ZINC ION × 2 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.4；pH 6.4
Resolution 1.90 Å R-free 0.232
1ZNI INSULIN Deposited 1997-09-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded ZN ZINC ION × 2 CL CHLORIDE ION × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.50 Å
1ZNI INSULIN Deposited 1997-09-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.50 Å
1ZNI INSULIN Deposited 1997-09-23 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 9 CL CHLORIDE ION × 9 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.50 Å
1ZNI INSULIN Deposited 1997-09-23 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 9 CL CHLORIDE ION × 9 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.50 Å
1ZNI INSULIN Deposited 1997-09-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 3 CL CHLORIDE ION × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.50 Å
1ZNI INSULIN Deposited 1997-09-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 3 CL CHLORIDE ION × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.50 Å
1ZNI INSULIN Deposited 1997-09-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 7 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded ZN ZINC ION × 6 CL CHLORIDE ION × 9 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.50 Å
1ZNI INSULIN Deposited 1997-09-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 8 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.50 Å
2EFA Neutron crystal structure of cubic insulin at pD6.6 Deposited 2007-02-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa） Fragment：Insulin A chain
Chain B 25–54（30 aa） Fragment：Insulin B chain
Not recorded No recorded non-water small molecule NEUTRON DIFFRACTION
X-ray crystallization conditions MICRODIALYSIS；pH 6.6；298 K；0.2M Sodium phosphate, 8mM 3NaEDTA, pH 6.6(PD), MICRODIALYSIS, temperature 298K
Resolution 2.70 Å R-free 0.291
2EFA Neutron crystal structure of cubic insulin at pD6.6 Deposited 2007-02-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa） Fragment：Insulin A chain
Chain B 25–54（30 aa） Fragment：Insulin B chain
Not recorded No recorded non-water small molecule NEUTRON DIFFRACTION
X-ray crystallization conditions MICRODIALYSIS；pH 6.6；298 K；0.2M Sodium phosphate, 8mM 3NaEDTA, pH 6.6(PD), MICRODIALYSIS, temperature 298K
Resolution 2.70 Å R-free 0.291
2G4M Insulin collected at 2.0 A wavelength Deposited 2006-02-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å R-free 0.223
2G4M Insulin collected at 2.0 A wavelength Deposited 2006-02-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å R-free 0.223
2TCI X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL Deposited 1995-09-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded SCN THIOCYANATE ION × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å
2TCI X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL Deposited 1995-09-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å
2ZPP Neutron crystal structure of cubic insulin at pD9 Deposited 2008-07-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded No recorded non-water small molecule NEUTRON DIFFRACTION
X-ray crystallization conditions MICRODIALYSIS；pH 9；298 K；0.2M sodium phosphate, 10mM 3NaEDTA, pH 9.0, MICRODIALYSIS, temperature 298K
Resolution 2.50 Å R-free 0.260
3GKY The Structural Basis of an ER Stress-Associated Bottleneck in a Protein Folding Landscape Deposited 2009-03-11 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 6 CL CHLORIDE ION × 6 IPH PHENOL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；0.02 M Tris, 0.05 M sodium citrate, 5% acetone, 0.03% phenol, 0.01% zinc acetate, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.80 Å R-free 0.253
3INS STRUCTURE OF INSULIN. RESULTS OF JOINT NEUTRON AND X-RAY REFINEMENT Deposited 1988-10-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded ZN ZINC ION × 1 Experimental method not declared mmCIF provides none of the parsed conditions Resolution not provided
3INS STRUCTURE OF INSULIN. RESULTS OF JOINT NEUTRON AND X-RAY REFINEMENT Deposited 1988-10-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 1 Experimental method not declared mmCIF provides none of the parsed conditions Resolution not provided
3INS STRUCTURE OF INSULIN. RESULTS OF JOINT NEUTRON AND X-RAY REFINEMENT Deposited 1988-10-14 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 6 Experimental method not declared mmCIF provides none of the parsed conditions Resolution not provided
3INS STRUCTURE OF INSULIN. RESULTS OF JOINT NEUTRON AND X-RAY REFINEMENT Deposited 1988-10-14 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 6 Experimental method not declared mmCIF provides none of the parsed conditions Resolution not provided
3MTH X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL Deposited 1995-09-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded MPB 4-HYDROXY-BENZOIC ACID METHYL ESTER × 1 ZN ZINC ION × 1 CL CHLORIDE ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å
3MTH X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL Deposited 1995-09-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 1 CL CHLORIDE ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å
3MTH X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL Deposited 1995-09-13 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded MPB 4-HYDROXY-BENZOIC ACID METHYL ESTER × 3 ZN ZINC ION × 6 CL CHLORIDE ION × 6 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å
3MTH X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL Deposited 1995-09-13 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded MPB 4-HYDROXY-BENZOIC ACID METHYL ESTER × 1 ZN ZINC ION × 2 CL CHLORIDE ION × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å
3MTH X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL Deposited 1995-09-13 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded MPB 4-HYDROXY-BENZOIC ACID METHYL ESTER × 1 ZN ZINC ION × 2 CL CHLORIDE ION × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å
3MTH X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL Deposited 1995-09-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 3 CL CHLORIDE ION × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å
3MTH X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL Deposited 1995-09-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 7 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded MPB 4-HYDROXY-BENZOIC ACID METHYL ESTER × 3 ZN ZINC ION × 3 CL CHLORIDE ION × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å
3RTO Acoustically mounted porcine insulin microcrystals yield an X-ray SAD structure Deposited 2011-05-03 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric
Chain A 88–108（21 aa） Fragment：Insulin A chain (UNP residues 88-108)
Chain B 25–54（30 aa） Fragment：Insulin B chain (UNP residues 25-54)
Chain C 88–108（21 aa） Fragment：Insulin A chain (UNP residues 88-108)
Chain D 25–54（30 aa） Fragment：Insulin B chain (UNP residues 25-54)
Not recorded ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions SMALL TUBES；pH 6；Microcrystals are obtained by dissolving 0.025 g protein in 5 mL of crystallizing solution (200 mL 0.02 M HCl, 100 mL 0.20 M sodium citrate, 60 mL acetone, 20 mL water, 20 mL 0.12 M zinc sulfate) at 315K. The solution is then rapidly quenched to 293K by immersing it in a cool water bath. Quenching speed determines the resulting crystal size. 20-micron crystals are obtained by quenching in a 293K water bath, 10-micron crystals by quenching in a 283K water bath, and 5-micron crystals by quenching in ice water. pH 6.0, SMALL TUBES
Resolution 1.80 Å R-free 0.223
3T2A TMAO-grown cubic insulin (porcine) Deposited 2011-07-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded TMO trimethylamine oxide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；Protein: 10 mg/mL in 50 mM CAPS pH 11; Well: 1.2 M TMAO, 0.1 M malate, pH 5.5 , VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.10 Å R-free 0.223
3T2A TMAO-grown cubic insulin (porcine) Deposited 2011-07-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded TMO trimethylamine oxide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；Protein: 10 mg/mL in 50 mM CAPS pH 11; Well: 1.2 M TMAO, 0.1 M malate, pH 5.5 , VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.10 Å R-free 0.223
4A7E X-ray crystal structure of porcine insulin flash-cooled at high pressure Deposited 2011-11-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 10；0.5 M SODIUM PHOSPHATE, 0.01 M EDTA, PH 10.0
Resolution 1.86 Å R-free 0.217
4INS THE STRUCTURE OF 2ZN PIG INSULIN CRYSTALS AT 1.5 ANGSTROMS RESOLUTION Deposited 1989-07-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.50 Å
4INS THE STRUCTURE OF 2ZN PIG INSULIN CRYSTALS AT 1.5 ANGSTROMS RESOLUTION Deposited 1989-07-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.50 Å
4INS THE STRUCTURE OF 2ZN PIG INSULIN CRYSTALS AT 1.5 ANGSTROMS RESOLUTION Deposited 1989-07-10 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 6 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.50 Å
4INS THE STRUCTURE OF 2ZN PIG INSULIN CRYSTALS AT 1.5 ANGSTROMS RESOLUTION Deposited 1989-07-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.50 Å
4INS THE STRUCTURE OF 2ZN PIG INSULIN CRYSTALS AT 1.5 ANGSTROMS RESOLUTION Deposited 1989-07-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded ZN ZINC ION × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.50 Å
4INS THE STRUCTURE OF 2ZN PIG INSULIN CRYSTALS AT 1.5 ANGSTROMS RESOLUTION Deposited 1989-07-10 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.50 Å
4INS THE STRUCTURE OF 2ZN PIG INSULIN CRYSTALS AT 1.5 ANGSTROMS RESOLUTION Deposited 1989-07-10 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 7 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.50 Å
5D52 In meso in situ serial X-ray crystallography structure of insulin at room temperature Deposited 2015-08-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded PO4 PHOSPHATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；293 K；0.1-0.2 M sodium phosphate, pH 5.5-6.1, and 33-38 %(w/v) PEG400
Resolution 1.80 Å R-free 0.214
5D53 In meso in situ serial X-ray crystallography structure of insulin at 100 K Deposited 2015-08-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded PO4 PHOSPHATE ION × 2 PE5 3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；293 K；0.1-0.2 M sodium phosphate, pH 5.5-6.1, and 33-38 %(w/v) PEG400
Resolution 1.50 Å R-free 0.170
5D54 In meso X-ray crystallography structure of insulin at 100 K Deposited 2015-08-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded PO4 PHOSPHATE ION × 2 PE5 3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；293 K；0.1-0.2 M sodium phosphate, pH 5.5-6.1, and 33-38 %(w/v) PEG400
Resolution 1.50 Å R-free 0.188
5D5E In meso in situ serial X-ray crystallography structure of insulin by sulfur-SAD at 100 K Deposited 2015-08-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded PO4 PHOSPHATE ION × 2 PE5 3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；293 K；0.1-0.2 M sodium phosphate, pH 5.5-6.1, and 33-38 %(w/v) PEG400
Resolution 2.41 Å R-free 0.220
5FB6 Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering Deposited 2015-12-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；0.6 M Na2HPO4 0.01 M EDTA
Resolution 1.90 Å R-free 0.173
5LIS Insulin solved by Native SAD from a dataset collected in one second Deposited 2016-07-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；30% ethylene glycol
Resolution 2.29 Å R-free 0.262
6INS X-RAY ANALYSIS OF THE SINGLE CHAIN B29-A1 PEPTIDE-LINKED INSULIN MOLECULE. A COMPLETELY INACTIVE ANALOGUE Deposited 1992-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain E 25–53（29 aa）
Chain E 88–108（21 aa）
Chain F 25–53（29 aa）
Chain F 88–108（21 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
6INS X-RAY ANALYSIS OF THE SINGLE CHAIN B29-A1 PEPTIDE-LINKED INSULIN MOLECULE. A COMPLETELY INACTIVE ANALOGUE Deposited 1992-11-25 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain E 25–53（29 aa）
Chain E 88–108（21 aa）
Chain F 25–53（29 aa）
Chain F 88–108（21 aa）
Not recorded ZN ZINC ION × 6 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
6Z7Z Porcine insulin in complex with the analytical antibody OXI-005 Fab Deposited 2020-06-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain G 88–108（21 aa）
Chain H 25–54（30 aa）
Not recorded MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M magnesium chloride, 20 % (w/v) PEG 3350
Resolution 2.40 Å R-free 0.259
6Z7Z Porcine insulin in complex with the analytical antibody OXI-005 Fab Deposited 2020-06-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain E 88–108（21 aa）
Chain F 25–54（30 aa）
Not recorded MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M magnesium chloride, 20 % (w/v) PEG 3350
Resolution 2.40 Å R-free 0.259
7AC4 Structure of insulin collected by rotation serial crystallography on a COC membrane at a synchrotron source Deposited 2020-09-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded PGR R-1,2-PROPANEDIOL × 1 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；50mM Na2HPO4, 10mM EDTA pH 10.8
Resolution 1.46 Å R-free 0.180
7INS STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z Deposited 1991-09-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded CRS M-CRESOL × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
7INS STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z Deposited 1991-09-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 10 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 25–54（30 aa）
Chain D 25–54（30 aa）
Chain F 25–54（30 aa）
Not recorded ZN ZINC ION × 1 UNL UNKNOWN LIGAND × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
7INS STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z Deposited 1991-09-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 11 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded CRS M-CRESOL × 2 ZN ZINC ION × 2 UNL UNKNOWN LIGAND × 4 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
7INS STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z Deposited 1991-09-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 12 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded CRS M-CRESOL × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
7INS STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z Deposited 1991-09-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Not recorded CRS M-CRESOL × 1 ZN ZINC ION × 1 UNL UNKNOWN LIGAND × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
7INS STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z Deposited 1991-09-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain E 88–108（21 aa）
Chain F 25–54（30 aa）
Not recorded CRS M-CRESOL × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
7INS STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z Deposited 1991-09-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Chain E 88–108（21 aa）
Chain F 25–54（30 aa）
Not recorded CRS M-CRESOL × 6 ZN ZINC ION × 2 UNL UNKNOWN LIGAND × 4 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
7INS STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z Deposited 1991-09-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Chain E 88–108（21 aa）
Chain F 25–54（30 aa）
Not recorded CRS M-CRESOL × 3 ZN ZINC ION × 1 UNL UNKNOWN LIGAND × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
7INS STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z Deposited 1991-09-03 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Chain E 88–108（21 aa）
Chain F 25–54（30 aa）
Not recorded CRS M-CRESOL × 4 ZN ZINC ION × 2 UNL UNKNOWN LIGAND × 4 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
7INS STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z Deposited 1991-09-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 7 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded CRS M-CRESOL × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
7INS STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z Deposited 1991-09-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 8 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 25–54（30 aa）
Chain D 25–54（30 aa）
Chain F 25–54（30 aa）
Not recorded ZN ZINC ION × 2 UNL UNKNOWN LIGAND × 4 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
7INS STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z Deposited 1991-09-03 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 9 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain C 88–108（21 aa）
Chain D 25–54（30 aa）
Chain E 88–108（21 aa）
Chain F 25–54（30 aa）
Not recorded CRS M-CRESOL × 2 ZN ZINC ION × 1 UNL UNKNOWN LIGAND × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
9INS MONOVALENT CATION BINDING IN CUBIC INSULIN CRYSTALS Deposited 1991-10-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.70 Å
9INS MONOVALENT CATION BINDING IN CUBIC INSULIN CRYSTALS Deposited 1991-10-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 88–108（21 aa）
Chain B 25–54（30 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.70 Å

41 other PDB entries and 126 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name INS_PIG
Isoform —
PDB entities 1, 2
Chains and sequence ranges Author chain A； PDBConstruct 1–21； UniProt 88–108 Author chain C； PDBConstruct 1–21； UniProt 88–108 Author chain B； PDBConstruct 1–30； UniProt 25–54 Author chain D； PDBConstruct 1–30； UniProt 25–54

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 3fhp

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 3fhp
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2. Structure Basics 2. Structure Basics

Entry ID entry_id3fhp
Deposition date deposition_date2008-12-09
Structure title titleA neutron crystallographic analysis of a porcine 2Zn insulin at 2.0 A resolution
Keywords keywords;2Zn insulin, neutron crystallography, protonation, H/D exchange, Carbohydrate metabolism, Cleavage on pair of basic residues, Glucose metabolism, Hormone, Secreted ;; HORMONE
Experimental Method methodNEUTRON DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier15.43 Å
Radius of gyration Rg (electron density) rg_electron14.16 Å
Forward intensity I(0) i04009010.00
Molecular weight molecular_weight13595.0 kDa
Excluded volume excluded_volume16555 ų
Envelope volume envelope_volume21434 ų
Hydration-shell volume shell_volume12602 ų
Envelope diameter envelope_diameter52.2 Å
Shell Rg shell_rg20.15 Å
Envelope Rg envelope_rg15.00 Å
Shape Rg shape_rg14.59 Å
Total Rg total_rg14.28 Å
Total atoms total_atoms1818
Residues n_residues102
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax53.9 Å
Rg (real space) rg_real15.38 Å
Rg uncertainty (real space) rg_real_error0.35 Å
I(0) (real space) i0_real4.0090e+06
I(0) uncertainty (real space) i0_real_error4.6240e+04
Rg (reciprocal space) rg_reciprocal15.38 Å
I(0) (reciprocal space) i0_reciprocal4009000.0000
Solution quality estimate total_estimate0.7800
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary19.1 Å
Skewness Skewness skewness0.261
Kurtosis Kurtosis kurtosis-0.237
Angular range angular_range— – 0.5000 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha628600.0000
Real-space data points n_real_points80
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.718; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.981; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (4)

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8. Citations (1)

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9. Files and Curves (10)