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3h11

Zymogen caspase-8:c-FLIPL protease domain complex

Method: X-RAY DIFFRACTION Dmax: 69.2 Å Quality: EXCELLENT
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1. Protein Identity and Related Structures Protein Identity & Related Structures

CASP8 and FADD-like apoptosis regulator

Homo sapiens

UniProt O15519

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count Chain A； UniProt 209–480 Not recorded Caspase-8 × 1 （Q14790） IETD aldehyde inhibitor × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 10.5；293 K；0.9 M sodium dihydrogen phosphate, 0.8 M dipotassium hydrogen phosphate, 0.1 M N-cyclohexyl-3-aminopropanesulfonic acid (CAPS), 0.2 M lithium sulfate, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K Resolution 1.90 Å R-free 0.250

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 3H11 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2N5R NMR structure of cFLIP-derived calmodulin binding peptide Deposited 2015-07-24 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 62–73（12 aa） Fragment：DED 1 domain residues 62-73
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；288 K；Ionic strength (raw mmCIF value) 0.15；Pressure ambient
NMR sample composition 20 mM potassium phosphate, 150 mM sodium chloride, 1 mM calcium chloride, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
3H13 c-FLIPL protease-like domain Deposited 2009-04-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 209–480（272 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；290 K；0.1 M Mes, 18% PEG, 5000 monomethyl ether, 0.1 M ammonium sulfate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K
Resolution 2.20 Å R-free 0.220
6M6O NMR SOLUTION STRUCTURE OF A C-FLIPs Deposited 2020-03-16 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–173（172 aa）
Mutation：F114G No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.5；298 K；Ionic strength (raw mmCIF value) 110；Pressure 1
NMR sample composition 0.6 nM [U-99% 13C; U-99% 15N] c-FLIPS, 1.0 nM EDTA-Na2, 1.0 nM TCEP, 0.02 % NaN3, 60 nM sodium phosphate, 50 mM sodium chloride, 95% H2O/5% D2O | 95% H2O/5% D2O
Resolution not provided
7DEE Structural Basis of the regulation of DISC Assembly by the interaction of c-FLIPs with Procaspase-8 Deposited 2020-11-03 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 2–173（172 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.4；298 K；Ionic strength (raw mmCIF value) 110；Pressure 1
NMR measurement conditions pH 7.4；298 K；Ionic strength (raw mmCIF value) 60；Pressure 1
NMR sample composition 10 mM C8-H1a, 0.002 % v/v sodium azide, 110 mM sodium phosphate, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition 04 mM C8-H1a, 0.002 % v/v sodium azide, 110 mM sodium phosphate, 0.6 mM [U-99% 13C; U-99% 15N] c-FLIPs, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition 0.002 % v/v sodium azide, 110 mM sodium phosphate, 0.6 mM [U-99% 13C; U-99% 15N] c-FLIPs, 1 mM TCEP, 1 mM EDTA, 95% H2O/5% D2O | 95% H2O/5% D2O
Resolution not provided
8YBX Structure of the FADD/Caspase-8/cFLIP death effector domain assembly Deposited 2024-02-16 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain H 1–181（181 aa）
Chain I 1–181（181 aa）
Chain J 1–181（181 aa）
Chain K 1–181（181 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.68 Å
8YD7 Structure of FADD/Caspase-8/cFLIP death effector domain assembly Deposited 2024-02-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain G 1–181（181 aa）
Chain H 1–181（181 aa）
Chain I 1–181（181 aa）
Chain K 1–181（181 aa）
Mutation：H7G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：H7G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：H7G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：H7G Non-standard monomer：Yes (specific site not provided by mmCIF) SE SELENIUM ATOM × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293.15 K；HEPES, TBG, PEG8000, TCEP, sodium chloride
Resolution 3.32 Å R-free 0.231
8YD8 Structure of FADD/Caspase-8/cFLIP death effector domain assembly Deposited 2024-02-19 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain H 1–181（181 aa）
Chain I 1–181（181 aa）
Chain J 1–181（181 aa）
Chain K 1–181（181 aa）
Mutation：H7G Mutation：H7G Mutation：H7G Mutation：H7G No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293.15 K；HEPES, PEG 8000, TBG, TCEP, sodium chloride
Resolution 3.11 Å R-free 0.241
8YM4 Structure of Caspase-8/cFLIP death effector domain assembly Deposited 2024-03-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain F 1–181（181 aa）
Chain G 1–181（181 aa）
Chain H 1–181（181 aa）
Chain I 1–181（181 aa）
Chain J 1–181（181 aa）
Chain K 1–181（181 aa）
Mutation：H7G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：H7G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：H7G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：H7G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：H7G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：H7G Non-standard monomer：Yes (specific site not provided by mmCIF) SE SELENIUM ATOM × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293.15 K；0.1 M MES pH 6.5, 0.08 M sodium chloride, 0.2 M Potassium Thiocyanate, 10 % PEG 4000, 0.01 M TCEP
Resolution 2.34 Å R-free 0.253
8YM5 Structure of Caspase-8/cFLIP death effector domain assembly Deposited 2024-03-08 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain F 1–181（181 aa）
Chain G 1–181（181 aa）
Chain H 1–181（181 aa）
Chain I 1–181（181 aa）
Chain J 1–181（181 aa）
Chain K 1–181（181 aa）
Mutation：H7G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：H7G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：H7G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：H7G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：H7G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：H7G Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293.15 K；0.1 M MES pH 6.5, 0.08 M sodium chloride, 0.2 M Potassium Thiocyanate, 10 % PEG 4000, 0.01 M TCEP
Resolution 2.09 Å R-free 0.231
8YM6 Structure of Caspase-8/cFLIP death effector domain assembly Deposited 2024-03-08 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain F 1–181（181 aa）
Chain G 1–181（181 aa）
Chain H 1–181（181 aa）
Chain I 1–181（181 aa）
Chain J 1–181（181 aa）
Chain K 1–181（181 aa）
Chain M 1–181（181 aa）
Chain N 1–181（181 aa）
Chain O 1–181（181 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293.15 K；0.1 M HEPES pH 7.5, 0.2 M Lithium chloride, 20 % PEG 400, 0.1 M TBG pH 9.0
Resolution 3.30 Å R-free 0.264
8YNI Structure of the FADD/Caspase-8/cFLIP death effector domain assembly Deposited 2024-03-11 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: 11-meric
Chain G 1–181（181 aa）
Chain H 1–181（181 aa）
Chain I 1–181（181 aa）
Chain J 1–181（181 aa）
Chain K 1–181（181 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.66 Å
8YNK Structure of the Caspase-8/cFLIP death effector domain assembly Deposited 2024-03-11 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain G 1–181（181 aa）
Chain H 1–181（181 aa）
Chain I 1–181（181 aa）
Chain J 1–181（181 aa）
Chain K 1–181（181 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.62 Å
8YNL Structure of the Caspase-8/cFLIP death effector domain assembly Deposited 2024-03-11 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain F 1–181（181 aa）
Chain G 1–181（181 aa）
Chain H 1–181（181 aa）
Chain I 1–181（181 aa）
Chain J 1–181（181 aa）
Chain K 1–181（181 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.55 Å
8YNM Structure of the Caspase-8/cFLIP death effector domain assembly Deposited 2024-03-11 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric
Chain F 1–181（181 aa）
Chain G 1–181（181 aa）
Chain H 1–181（181 aa）
Chain I 1–181（181 aa）
Chain J 1–181（181 aa）
Chain K 1–181（181 aa）
Chain N 1–181（181 aa）
Chain O 1–181（181 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
8YNN Structure of the Caspase-8/cFLIP death effector domain assembly Deposited 2024-03-11 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain H 1–181（181 aa）
Chain I 1–181（181 aa）
Chain J 1–181（181 aa）
Chain K 1–181（181 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.97 Å

15 other PDB entries and 15 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CFLAR_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–272； UniProt 209–480

Caspase-8

Homo sapiens

UniProt Q14790

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count Chain B； UniProt 217–479 Mutation：D359A, D369A CASP8 and FADD-like apoptosis regulator × 1 （O15519） IETD aldehyde inhibitor × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 10.5；293 K；0.9 M sodium dihydrogen phosphate, 0.8 M dipotassium hydrogen phosphate, 0.1 M N-cyclohexyl-3-aminopropanesulfonic acid (CAPS), 0.2 M lithium sulfate, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K Resolution 1.90 Å R-free 0.250

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 3H11 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1F9E CASPASE-8 SPECIFICITY PROBED AT SUBSITE S4: CRYSTAL STRUCTURE OF THE CASPASE-8-Z-DEVD-CHO Deposited 2000-07-10 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 222–374（153 aa）
Chain B 390–478（89 aa）
Chain C 222–374（153 aa）
Chain D 390–478（89 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；1-propanol, sodium citrate, MES , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 4.0K, temperature 277K
Resolution 2.90 Å R-free 0.289
1F9E CASPASE-8 SPECIFICITY PROBED AT SUBSITE S4: CRYSTAL STRUCTURE OF THE CASPASE-8-Z-DEVD-CHO Deposited 2000-07-10 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 222–374（153 aa）
Chain F 390–478（89 aa）
Chain G 222–374（153 aa）
Chain H 390–478（89 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；1-propanol, sodium citrate, MES , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 4.0K, temperature 277K
Resolution 2.90 Å R-free 0.289
1F9E CASPASE-8 SPECIFICITY PROBED AT SUBSITE S4: CRYSTAL STRUCTURE OF THE CASPASE-8-Z-DEVD-CHO Deposited 2000-07-10 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain I 222–374（153 aa）
Chain J 390–478（89 aa）
Chain K 222–374（153 aa）
Chain L 390–478（89 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；1-propanol, sodium citrate, MES , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 4.0K, temperature 277K
Resolution 2.90 Å R-free 0.289
1I4E CRYSTAL STRUCTURE OF THE CASPASE-8/P35 COMPLEX Deposited 2001-02-20 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 222–479（258 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
Resolution 3.00 Å R-free 0.296
1I4E CRYSTAL STRUCTURE OF THE CASPASE-8/P35 COMPLEX Deposited 2001-02-20 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 222–479（258 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
Resolution 3.00 Å R-free 0.296
1QDU CRYSTAL STRUCTURE OF THE COMPLEX OF CASPASE-8 WITH THE TRIPEPTIDE KETONE INHIBITOR ZEVD-DCBMK Deposited 1999-07-10 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 222–374（153 aa）
Chain B 390–477（88 aa）
Chain C 222–374（153 aa）
Chain D 390–477（88 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.3；277 K；300mM ammonium phosphate, 27% (w/v) isopropanol, 100mM sodium phosphate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.80 Å R-free 0.302
1QDU CRYSTAL STRUCTURE OF THE COMPLEX OF CASPASE-8 WITH THE TRIPEPTIDE KETONE INHIBITOR ZEVD-DCBMK Deposited 1999-07-10 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 222–374（153 aa）
Chain F 390–477（88 aa）
Chain G 222–374（153 aa）
Chain H 390–477（88 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.3；277 K；300mM ammonium phosphate, 27% (w/v) isopropanol, 100mM sodium phosphate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.80 Å R-free 0.302
1QDU CRYSTAL STRUCTURE OF THE COMPLEX OF CASPASE-8 WITH THE TRIPEPTIDE KETONE INHIBITOR ZEVD-DCBMK Deposited 1999-07-10 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain I 222–374（153 aa）
Chain J 390–477（88 aa）
Chain K 222–374（153 aa）
Chain L 390–477（88 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.3；277 K；300mM ammonium phosphate, 27% (w/v) isopropanol, 100mM sodium phosphate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.80 Å R-free 0.302
1QTN CRYSTAL STRUCTURE OF THE COMPLEX OF CASPASE-8 WITH THE TETRAPEPTIDE INHIBITOR ACE-IETD-ALDEHYDE Deposited 1999-06-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 223–374（152 aa） Fragment：P18 FRAGMENT
Chain B 390–479（90 aa） Fragment：P11 FRAGMENT
Not recorded DTD DITHIANE DIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；282 K；3 MICROLITERS PROTEIN (8.4 MG/ ML IN 20 MM TRISHCL, 100MM DTT AT PH 8.0) MIXED WITH 3 MICROLITERS WELL BUFFER (1.4 SODIUM CITRATE, 0.1M HEPES, AT PH 8.0) AT 4 DEG. C, VAPOR DIFFUSION, SITTING DROP, temperature 282K
Resolution 1.20 Å R-free 0.188
2C2Z Crystal structure of caspase-8 in complex with aza-peptide Michael acceptor inhibitor Deposited 2005-10-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 218–374（157 aa） Fragment：ALPHA SUB-UNIT, RESIDUES 218-374
Chain B 376–479（104 aa） Fragment：BETA-SUBUNIT, RESIDUES 376-479
Not recorded DTD DITHIANE DIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8；1.4M SODIUM CITRATE, 100MM HEPES PH 8.0
Resolution 1.95 Å R-free 0.187
2FUN alternative p35-caspase-8 complex Deposited 2006-01-27 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 222–479（258 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；VAPOR DIFFUSION, HANGING DROP, pH 7, temperature 295K
Resolution 3.00 Å R-free 0.260
2FUN alternative p35-caspase-8 complex Deposited 2006-01-27 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 222–479（258 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；VAPOR DIFFUSION, HANGING DROP, pH 7, temperature 295K
Resolution 3.00 Å R-free 0.260
2K7Z Solution Structure of the Catalytic Domain of Procaspase-8 Deposited 2008-08-28 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 217–479（263 aa） Fragment：UNP residues 213-479
Mutation：C360A No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 8；305 K；Ionic strength (raw mmCIF value) 0.1；Pressure ambient
NMR sample composition 0.8 mM [U-99% 13C; U-99% 15N] procaspase8, 10 mM DTT, 100 mM sodium chloride, 20 mM [U-2H] TRIS, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 0.8 mM [U-100% 13C; U-100% 15N; 80% 2H] procaspase8, 10 mM DTT, 100 mM sodium chloride, 20 mM [U-2H] TRIS, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2Y1L Caspase-8 in Complex with DARPin-8.4 Deposited 2010-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: octameric
Chain A 217–374（158 aa） Fragment：P18 SUBUNIT, RESIDUES 218-374
Chain B 376–479（104 aa） Fragment：P10 SUBUNIT, RESIDUES 376-479
Chain C 217–374（158 aa） Fragment：P18 SUBUNIT, RESIDUES 218-374
Chain D 376–479（104 aa） Fragment：P10 SUBUNIT, RESIDUES 376-479
Not recorded SO4 SULFATE ION × 3 EDO 1,2-ETHANEDIOL × 5 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.8；100 MM CITRIC ACID PH 4.9 (RT), 200 MM LI2SO4, 22.5% PEG 4K.
Resolution 1.80 Å R-free 0.218
3KJN Caspase 8 bound to a covalent inhibitor Deposited 2009-11-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 211–374（164 aa） Fragment：residues 211-374
Chain B 385–479（95 aa） Fragment：residues 385-479
Not recorded DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 2 B93 (3S)-3-({[(5S)-2-{2-[(1H-benzimidazol-5-ylcarbonyl)amino]ethyl}-7-(cyclohexylmethyl)-1,3-dioxo-2,3,5,8-tetrahydro-1H-[1,2,4]triazolo[1,2-a]pyridazin-5-yl]carbonyl}amino)-4-oxopentanoic acid × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.5；277 K；2 microliters of 100 mM inhibitor stock in DMSO was added to 100 microliters of 8.4 mg/mL protein in 20 mM Tris, 100 mM DTT, pH 8.0. Protein solution was mixed with an equal volume of well solution (1.0-1.1 M Citrate, 50 mM HEPES or PIPES pH 6.5, 25 mM DTT), VAPOR DIFFUSION, temperature 277K
Resolution 1.80 Å R-free 0.202
3KJQ Caspase 8 with covalent inhibitor Deposited 2009-11-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 211–374（164 aa）
Chain B 385–479（95 aa）
Not recorded B94 (3S)-3-({[(5S,8R)-2-(3-carboxypropyl)-8-(2-{[(4-chlorophenyl)acetyl]amino}ethyl)-1,3-dioxo-2,3,5,8-tetrahydro-1H-[1,2,4]triazolo[1,2-a]pyridazin-5-yl]carbonyl}amino)-4-oxopentanoic acid × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.8；277 K；2 microliters of 100 mM inhibitor stock in DMSO was added to 100 microliters of 8.4 mg/mL protein in 20 mM Tris, 100 mM DTT, pH 8.0. Protein solution was mixed with an equal volume of well solution (1.0-1.1 M Citrate, 50 mM HEPES or PIPES pH 6.5, 25 mM DTT), VAPOR DIFFUSION, temperature 277K
Resolution 1.80 Å R-free 0.207
4JJ7 Caspase-3 specific unnatural amino acid-based peptides Deposited 2013-03-07 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 217–479（263 aa）
Not recorded DTD DITHIANE DIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；295 K；1 M Sodium citrate, 0.1M HEPES pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 1.18 Å R-free 0.157
4PRZ Caspase-8 specific unnatural amino acid peptides Deposited 2014-03-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 217–479（263 aa） Fragment：UNP RESIDUES 217-479
Not recorded DTD DITHIANE DIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.4；295 K；1.0 M sodium citrate, 0.1 M HEPES, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 2.12 Å R-free 0.218
4PS1 Caspase-8 specific unnatural amino acid peptides Deposited 2014-03-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 217–479（263 aa） Fragment：UNP RESIDUES 217-479
Not recorded DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；295 K；0.1 M Sodium citrate, pH 5.5, 36% PEG600, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 1.73 Å R-free 0.185
4PS1 Caspase-8 specific unnatural amino acid peptides Deposited 2014-03-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 217–479（263 aa） Fragment：UNP RESIDUES 217-479
Not recorded DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；295 K；0.1 M Sodium citrate, pH 5.5, 36% PEG600, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 1.73 Å R-free 0.185
4PS1 Caspase-8 specific unnatural amino acid peptides Deposited 2014-03-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 217–479（263 aa） Fragment：UNP RESIDUES 217-479
Not recorded DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；295 K；0.1 M Sodium citrate, pH 5.5, 36% PEG600, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 1.73 Å R-free 0.185
4PS1 Caspase-8 specific unnatural amino acid peptides Deposited 2014-03-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 217–479（263 aa） Fragment：UNP RESIDUES 217-479
Not recorded DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；295 K；0.1 M Sodium citrate, pH 5.5, 36% PEG600, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 1.73 Å R-free 0.185
4ZBW Crystal structure of death effector domain of Caspase8 in Homo Sapiens Deposited 2015-04-15 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 61–247（187 aa）
Chain B 61–247（187 aa）
Mutation：F122A, I128D Mutation：F122A, I128D No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 8.5；293 K；0.2M sodium chloride, 25% PEG3350
Resolution 2.20 Å R-free 0.251
5H31 Structural basis for dimerization of the death effector domains of Caspase-8 Deposited 2016-10-19 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–188（188 aa） Fragment：UNP residues 1-188
Chain B 1–188（188 aa） Fragment：UNP residues 1-188
Mutation：F122A Mutation：F122A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 8.5；293 K；100 mM sodium chloride, 100 mM Tris, 21% PEG3350, 10 mM Sarcosine,
Resolution 3.17 Å R-free 0.268
5H31 Structural basis for dimerization of the death effector domains of Caspase-8 Deposited 2016-10-19 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 1–188（188 aa） Fragment：UNP residues 1-188
Chain D 1–188（188 aa） Fragment：UNP residues 1-188
Mutation：F122A Mutation：F122A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 8.5；293 K；100 mM sodium chloride, 100 mM Tris, 21% PEG3350, 10 mM Sarcosine,
Resolution 3.17 Å R-free 0.268
5H33 Structural basis for dimerization of the death effector domains of Caspase-8 Deposited 2016-10-20 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–188（188 aa）
Chain B 1–188（188 aa）
Mutation：F122A Mutation：F122A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 8.5；293 K；50mM sodium chloride, 100mM Tris pH 8.5, 22.5% PEG3350
Resolution 3.60 Å R-free 0.313
5JQE Crystal structure of caspase8 tDED Deposited 2016-05-04 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–186（186 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 8；293 K；0.1M MMT buffer, pH 8.0, 25% PEG 1500
Resolution 3.16 Å R-free 0.274
5L08 Cryo-EM structure of Casp-8 tDED filament Deposited 2016-07-26 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 9 PDB declaration: nonameric
Chain A 60–243（184 aa）
Chain B 60–243（184 aa）
Chain C 60–243（184 aa）
Chain D 60–243（184 aa）
Chain E 60–243（184 aa）
Chain F 60–243（184 aa）
Chain G 60–243（184 aa）
Chain H 60–243（184 aa）
Chain I 60–243（184 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
6AGW Pro-domain of Caspase-8 Deposited 2018-08-15 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–180（180 aa） Fragment：UNP residues 1-180
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；(NH4)2SO4, Cacodylate, NaCl
Resolution 2.09 Å R-free 0.269
6PX9 Crystal structure of procaspase-8 in complex with covalent small molecule inhibitor 63-R Deposited 2019-07-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 276–538（263 aa） Fragment：UNP residues 276-538
Chain B 276–538（263 aa） Fragment：UNP residues 276-538
Mutation：D374A, D384A, C409S, C433S Mutation：D374A, D384A, C409S, C433S 63R N-{(3R)-1-[4-(morpholin-4-yl)benzene-1-carbonyl]piperidin-3-yl}-N-phenylacetamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；0.1 M imidazole, pH 8.0, 1.0 M sodium citrate
Resolution 2.88 Å R-free 0.366
6PX9 Crystal structure of procaspase-8 in complex with covalent small molecule inhibitor 63-R Deposited 2019-07-25 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 276–538（263 aa） Fragment：UNP residues 276-538
Chain D 276–538（263 aa） Fragment：UNP residues 276-538
Mutation：D374A, D384A, C409S, C433S Mutation：D374A, D384A, C409S, C433S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；0.1 M imidazole, pH 8.0, 1.0 M sodium citrate
Resolution 2.88 Å R-free 0.366
6PX9 Crystal structure of procaspase-8 in complex with covalent small molecule inhibitor 63-R Deposited 2019-07-25 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain E 276–538（263 aa） Fragment：UNP residues 276-538
Chain F 276–538（263 aa） Fragment：UNP residues 276-538
Mutation：D374A, D384A, C409S, C433S Mutation：D374A, D384A, C409S, C433S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；0.1 M imidazole, pH 8.0, 1.0 M sodium citrate
Resolution 2.88 Å R-free 0.366
6X8H Caspase-8 in complex with AOMK inhibitor, Ac-DW3-KE, forms tetrahedral adduct Deposited 2020-06-01 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 276–443（168 aa） Fragment：p18 (UNP residues 276-443)
Chain B 444–538（95 aa） Fragment：p10 (UNP residues 444-538)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.1；295 K；1:1 protein : 0.10 M HEPES, 1.0 M sodium citrate
Resolution 1.48 Å R-free 0.167
7LVM CASP8 isoform B DED domain Deposited 2021-02-25 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–188（187 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；294 K；100mM Hepes pH 7.5, 20% Ethylene Glycol, 18% PEG 3350, 200mM Sodium Chloride
Resolution 1.47 Å R-free 0.233
8YBX Structure of the FADD/Caspase-8/cFLIP death effector domain assembly Deposited 2024-02-16 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain A 1–479（479 aa）
Chain B 1–479（479 aa）
Chain C 1–479（479 aa）
Mutation：F122G/L123G, C360A, D374A, D384A Mutation：F122G/L123G, C360A, D374A, D384A Mutation：F122G/L123G, C360A, D374A, D384A No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.68 Å
8YD7 Structure of FADD/Caspase-8/cFLIP death effector domain assembly Deposited 2024-02-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain A 1–185（185 aa）
Chain B 1–185（185 aa）
Chain C 1–185（185 aa）
Chain D 1–185（185 aa）
Chain E 1–185（185 aa）
Mutation：F122G,L123G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：F122G,L123G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：F122G,L123G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：F122G,L123G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：F122G,L123G Non-standard monomer：Yes (specific site not provided by mmCIF) SE SELENIUM ATOM × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293.15 K；HEPES, TBG, PEG8000, TCEP, sodium chloride
Resolution 3.32 Å R-free 0.231
8YD8 Structure of FADD/Caspase-8/cFLIP death effector domain assembly Deposited 2024-02-19 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain A 1–185（185 aa）
Chain B 1–185（185 aa）
Chain C 1–185（185 aa）
Chain D 1–185（185 aa）
Chain E 1–185（185 aa）
Mutation：F122G, L123G Mutation：F122G, L123G Mutation：F122G, L123G Mutation：F122G, L123G Mutation：F122G, L123G No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293.15 K；HEPES, PEG 8000, TBG, TCEP, sodium chloride
Resolution 3.11 Å R-free 0.241
8YM4 Structure of Caspase-8/cFLIP death effector domain assembly Deposited 2024-03-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain A 1–185（185 aa）
Chain B 1–185（185 aa）
Chain C 1–185（185 aa）
Chain D 1–185（185 aa）
Mutation：F122G, L123G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：F122G, L123G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：F122G, L123G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：F122G, L123G Non-standard monomer：Yes (specific site not provided by mmCIF) SE SELENIUM ATOM × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293.15 K；0.1 M MES pH 6.5, 0.08 M sodium chloride, 0.2 M Potassium Thiocyanate, 10 % PEG 4000, 0.01 M TCEP
Resolution 2.34 Å R-free 0.253
8YM5 Structure of Caspase-8/cFLIP death effector domain assembly Deposited 2024-03-08 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain A 1–185（185 aa）
Chain B 1–185（185 aa）
Chain C 1–185（185 aa）
Chain D 1–185（185 aa）
Mutation：F122G, L123G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：F122G, L123G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：F122G, L123G Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：F122G, L123G Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293.15 K；0.1 M MES pH 6.5, 0.08 M sodium chloride, 0.2 M Potassium Thiocyanate, 10 % PEG 4000, 0.01 M TCEP
Resolution 2.09 Å R-free 0.231
8YM6 Structure of Caspase-8/cFLIP death effector domain assembly Deposited 2024-03-08 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain A 1–185（185 aa）
Chain B 1–185（185 aa）
Chain C 1–185（185 aa）
Chain D 1–185（185 aa）
Mutation：F122G, L123G Mutation：F122G, L123G Mutation：F122G, L123G Mutation：F122G, L123G No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293.15 K；0.1 M HEPES pH 7.5, 0.2 M Lithium chloride, 20 % PEG 400, 0.1 M TBG pH 9.0
Resolution 3.30 Å R-free 0.264
8YNI Structure of the FADD/Caspase-8/cFLIP death effector domain assembly Deposited 2024-03-11 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: 11-meric
Chain A 1–479（479 aa）
Chain B 1–479（479 aa）
Chain C 1–479（479 aa）
Mutation：F122G, L123G, C360A, D374A, D384A Mutation：F122G, L123G, C360A, D374A, D384A Mutation：F122G, L123G, C360A, D374A, D384A No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.66 Å
8YNK Structure of the Caspase-8/cFLIP death effector domain assembly Deposited 2024-03-11 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–479（479 aa）
Chain B 1–479（479 aa）
Chain C 1–479（479 aa）
Mutation：F122G, L123G, C360A, D374A, D384A Mutation：F122G, L123G, C360A, D374A, D384A Mutation：F122G, L123G, C360A, D374A, D384A No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.62 Å
8YNL Structure of the Caspase-8/cFLIP death effector domain assembly Deposited 2024-03-11 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 1–479（479 aa）
Chain B 1–479（479 aa）
Chain C 1–479（479 aa）
Mutation：F122G, L123G, C360A, D374A, D384A Mutation：F122G, L123G, C360A, D374A, D384A Mutation：F122G, L123G, C360A, D374A, D384A No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.55 Å
8YNM Structure of the Caspase-8/cFLIP death effector domain assembly Deposited 2024-03-11 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric
Chain A 1–479（479 aa）
Chain B 1–479（479 aa）
Chain C 1–479（479 aa）
Mutation：F122G, L123G, C360A, D374A, D384A Mutation：F122G, L123G, C360A, D374A, D384A Mutation：F122G, L123G, C360A, D374A, D384A No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
8YNN Structure of the Caspase-8/cFLIP death effector domain assembly Deposited 2024-03-11 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–479（479 aa）
Chain B 1–479（479 aa）
Chain C 1–479（479 aa）
Mutation：F122G, L123G, C360A, D374A, D384A Mutation：F122G, L123G, C360A, D374A, D384A Mutation：F122G, L123G, C360A, D374A, D384A No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.97 Å

33 other PDB entries and 45 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CASP8_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–263； UniProt 217–479

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 3h11

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 3h11
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2. Structure Basics 2. Structure Basics

Entry ID entry_id3h11
Deposition date deposition_date2009-04-10
Structure title titleZymogen caspase-8:c-FLIPL protease domain complex
Keywords keywords;cell death, apoptosis, Caspase, Alternative splicing, Host-virus interaction, Polymorphism, Cytoplasm, Disease mutation, Hydrolase, Phosphoprotein, Protease, Thiol protease, Zymogen ;; APOPTOSIS
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier22.89 Å
Radius of gyration Rg (electron density) rg_electron21.62 Å
Forward intensity I(0) i043487600.00
Molecular weight molecular_weight51234.0 kDa
Excluded volume excluded_volume64085 ų
Envelope volume envelope_volume72905 ų
Hydration-shell volume shell_volume27254 ų
Envelope diameter envelope_diameter71.4 Å
Shell Rg shell_rg29.18 Å
Envelope Rg envelope_rg21.81 Å
Shape Rg shape_rg21.61 Å
Total Rg total_rg22.49 Å
Total atoms total_atoms3595
Residues n_residues445
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax69.2 Å
Rg (real space) rg_real22.75 Å
Rg uncertainty (real space) rg_real_error0.33 Å
I(0) (real space) i0_real4.3490e+07
I(0) uncertainty (real space) i0_real_error4.7690e+05
Rg (reciprocal space) rg_reciprocal22.78 Å
I(0) (reciprocal space) i0_reciprocal43490000.0000
Solution quality estimate total_estimate0.9090
Solution quality rating solution_quality EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary28.8 Å
Skewness Skewness skewness0.138
Kurtosis Kurtosis kurtosis-0.517
Angular range angular_range— – 0.3450 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha8477000.0000
Real-space data points n_real_points66
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.944; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.988; Smooth: 0.993
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (4)

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7. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

Domain ID domain_idd3h11a_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.17 — Caspase-like
Superfamily Superfamily superfamilyc.17.1 — Caspase-like
Family Family familyc.17.1.1 — Caspase catalytic domain
Domain ID domain_idd3h11b_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.17 — Caspase-like
Superfamily Superfamily superfamilyc.17.1 — Caspase-like
Family Family familyc.17.1.1 — Caspase catalytic domain

CATH v4.4 (2 domains)

Domain ID domain_id3h11A00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily1460 —
Domain ID domain_id3h11B00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily1460 —
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8. Citations (1)

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9. Files and Curves (10)