3j7p

Structure of the 80S mammalian ribosome bound to eEF2

Method: ELECTRON MICROSCOPY D_max: 242.5 Å Quality: EXCELLENT

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Ribosomal protein uL2

OrganismNot specified

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain A； UniProt 1–257	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain A 1–257（257 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	F2Z567_PIG
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain A； PDBConstruct 1–257； UniProt 1–257

Ribosomal protein uL18

OrganismNot specified

UniProt F1S530

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain D； UniProt 1–297	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain D 1–297（297 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	F1S530_PIG
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain D； PDBConstruct 1–297； UniProt 1–297

Ribosomal protein eL8

OrganismNot specified

UniProt I3LVI8

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain G； UniProt 1–266	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain G 1–266（266 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	I3LVI8_PIG
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain G； PDBConstruct 1–266； UniProt 1–266

Ribosomal protein uL6

OrganismNot specified

UniProt I3LP78

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain H； UniProt 1–192	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain H 1–192（192 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7Q Structure of the idle mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 48-meric	Chain H 1–192（192 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain H 1–192（192 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	I3LP78_PIG
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain H； PDBConstruct 1–192； UniProt 1–192

Ribosomal protein uL5

OrganismNot specified

UniProt Q29205

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain J； UniProt 1–178	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain J 1–178（178 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7Q Structure of the idle mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 48-meric	Chain J 1–178（178 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain J 1–178（178 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL11_PIG
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain J； PDBConstruct 1–178； UniProt 1–178

Ribosomal protein uL11

OrganismNot specified

UniProt Q6QAS5

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain K； UniProt 27–189	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence

UniProt name	Q6QAS5_PIG
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain K； PDBConstruct 1–163； UniProt 27–189

Ribosomal protein eL13

OrganismNot specified

UniProt I3LSD3

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain L； UniProt 1–211	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain L 1–211（211 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	I3LSD3_PIG
Isoform	—
PDB entities	15
Chains and sequence ranges	Author chain L； PDBConstruct 1–211； UniProt 1–211

Ribosomal protein eL14

OrganismNot specified

UniProt A1XQU3

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain M； UniProt 1–213	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain M 1–213（213 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7Q Structure of the idle mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 48-meric	Chain M 1–213（213 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain M 1–213（213 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL14_PIG
Isoform	—
PDB entities	16
Chains and sequence ranges	Author chain M； PDBConstruct 1–213； UniProt 1–213

Ribosomal protein eL15

OrganismNot specified

UniProt I3LK33

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain N； UniProt 1–204	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain N 1–204（204 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	I3LK33_PIG
Isoform	—
PDB entities	17
Chains and sequence ranges	Author chain N； PDBConstruct 1–204； UniProt 1–204

Ribosomal protein uL13

OrganismNot specified

UniProt F1RI01

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain O； UniProt 1–204	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain O 1–204（204 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	F1RI01_PIG
Isoform	—
PDB entities	18
Chains and sequence ranges	Author chain O； PDBConstruct 1–204； UniProt 1–204

Ribosomal protein eL18

OrganismNot specified

UniProt I3L6F1

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain Q； UniProt 1–188	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain Q 1–188（188 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	I3L6F1_PIG
Isoform	—
PDB entities	20
Chains and sequence ranges	Author chain Q； PDBConstruct 1–188； UniProt 1–188

Ribosomal protein eL19

OrganismNot specified

UniProt F2Z546

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain R； UniProt 1–196	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain R 1–196（196 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	F2Z546_PIG
Isoform	—
PDB entities	21
Chains and sequence ranges	Author chain R； PDBConstruct 1–196； UniProt 1–196

Ribosomal protein eL20

OrganismNot specified

UniProt F1S935

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain S； UniProt 1–224	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain S 1–224（224 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	F1S935_PIG
Isoform	—
PDB entities	22
Chains and sequence ranges	Author chain S； PDBConstruct 1–224； UniProt 1–224

Ribosomal protein eL21

OrganismNot specified

UniProt F1RTJ9

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain T； UniProt 1–160	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain T 1–160（160 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	F1RTJ9_PIG
Isoform	—
PDB entities	23
Chains and sequence ranges	Author chain T； PDBConstruct 1–160； UniProt 1–160

Ribosomal protein eL22

OrganismNot specified

UniProt P67985

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain U； UniProt 1–128	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain U 1–128（128 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7Q Structure of the idle mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 48-meric	Chain U 1–128（128 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain U 1–128（128 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL22_PIG
Isoform	—
PDB entities	24
Chains and sequence ranges	Author chain U； PDBConstruct 1–128； UniProt 1–128

Ribosomal protein uL14

OrganismNot specified

UniProt P62831

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain V； UniProt 1–140	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain V 1–140（140 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL23_PIG
Isoform	—
PDB entities	25
Chains and sequence ranges	Author chain V； PDBConstruct 1–140； UniProt 1–140

Ribosomal protein uL23

OrganismNot specified

UniProt F2Z522

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain X； UniProt 1–156	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain X 1–156（156 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	F2Z522_PIG
Isoform	—
PDB entities	27
Chains and sequence ranges	Author chain X； PDBConstruct 1–156； UniProt 1–156

Ribosomal protein uL24

OrganismNot specified

UniProt I3L7Y1

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain Y； UniProt 1–145	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain Y 1–145（145 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	I3L7Y1_PIG
Isoform	—
PDB entities	28
Chains and sequence ranges	Author chain Y； PDBConstruct 1–145； UniProt 1–145

Ribosomal protein eL27

OrganismNot specified

UniProt A1XQU5

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain Z； UniProt 1–136	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain Z 1–136（136 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7Q Structure of the idle mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 48-meric	Chain Z 1–136（136 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain Z 1–136（136 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL27_PIG
Isoform	—
PDB entities	29
Chains and sequence ranges	Author chain Z； PDBConstruct 1–136； UniProt 1–136

Ribosomal protein uL15

OrganismNot specified

UniProt F1RGC0

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain a； UniProt 1–148	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain a 1–148（148 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	F1RGC0_PIG
Isoform	—
PDB entities	30
Chains and sequence ranges	Author chain a； PDBConstruct 1–148； UniProt 1–148

Ribosomal protein eL29

OrganismNot specified

UniProt Q95281

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain b； UniProt 1–160	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain b 1–160（160 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7Q Structure of the idle mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 48-meric	Chain b 1–160（160 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain b 1–160（160 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL29_PIG
Isoform	—
PDB entities	31
Chains and sequence ranges	Author chain b； PDBConstruct 1–160； UniProt 1–160

Ribosomal protein eL30

OrganismNot specified

UniProt F2Z554

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain c； UniProt 1–115	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain c 1–115（115 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	F2Z554_PIG
Isoform	—
PDB entities	32
Chains and sequence ranges	Author chain c； PDBConstruct 1–115； UniProt 1–115

Ribosomal protein eL31

OrganismNot specified

UniProt P62901

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain d； UniProt 1–125	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain d 1–125（125 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7Q Structure of the idle mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 48-meric	Chain d 1–125（125 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain d 1–125（125 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL31_PIG
Isoform	—
PDB entities	33
Chains and sequence ranges	Author chain d； PDBConstruct 1–125； UniProt 1–125

Ribosomal protein eL32

OrganismNot specified

UniProt Q6QAT0

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain e； UniProt 1–135	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain e 1–135（135 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7Q Structure of the idle mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 48-meric	Chain e 1–135（135 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain e 1–135（135 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL32_PIG
Isoform	—
PDB entities	34
Chains and sequence ranges	Author chain e； PDBConstruct 1–135； UniProt 1–135

Ribosomal protein eL33

OrganismNot specified

UniProt F2Z5Q8

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain f； UniProt 1–110	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain f 1–110（110 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	F2Z5Q8_PIG
Isoform	—
PDB entities	35
Chains and sequence ranges	Author chain f； PDBConstruct 1–110； UniProt 1–110

Ribosomal protein eL34

OrganismNot specified

UniProt F2Z5B9

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain g； UniProt 1–117	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain g 1–117（117 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	F2Z5B9_PIG
Isoform	—
PDB entities	36
Chains and sequence ranges	Author chain g； PDBConstruct 1–117； UniProt 1–117

Ribosomal protein uL29

OrganismNot specified

UniProt Q29361

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain h； UniProt 1–123	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain h 1–123（123 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7Q Structure of the idle mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 48-meric	Chain h 1–123（123 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain h 1–123（123 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL35_PIG
Isoform	—
PDB entities	37
Chains and sequence ranges	Author chain h； PDBConstruct 1–123； UniProt 1–123

Ribosomal protein eL36

OrganismNot specified

UniProt F2Z5K6

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain i； UniProt 1–105	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain i 1–105（105 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	F2Z5K6_PIG
Isoform	—
PDB entities	38
Chains and sequence ranges	Author chain i； PDBConstruct 1–105； UniProt 1–105

Ribosomal protein eL38

OrganismNot specified

UniProt F2Z568

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain k； UniProt 1–70	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain k 1–70（70 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7Q Structure of the idle mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 48-meric	Chain k 1–70（70 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain k 1–70（70 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	F2Z568_PIG
Isoform	—
PDB entities	40
Chains and sequence ranges	Author chain k； PDBConstruct 1–70； UniProt 1–70

Ribosomal protein eL39

OrganismNot specified

UniProt K7GP63

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain l； UniProt 1–51	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain l 1–51（51 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	K7GP63_PIG
Isoform	—
PDB entities	41
Chains and sequence ranges	Author chain l； PDBConstruct 1–51； UniProt 1–51

Ribosomal protein eL40

OrganismNot specified

UniProt P63053

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain m； UniProt 1–128	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain m 1–128（128 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7Q Structure of the idle mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 48-meric	Chain m 1–128（128 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain m 1–128（128 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL40_PIG
Isoform	—
PDB entities	42
Chains and sequence ranges	Author chain m； PDBConstruct 1–128； UniProt 1–128

Ribosomal protein eL42

OrganismNot specified

UniProt P83884

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain o； UniProt 1–106	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain o 1–106（106 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL36A_PIG
Isoform	—
PDB entities	44
Chains and sequence ranges	Author chain o； PDBConstruct 1–106； UniProt 1–106

Ribosomal protein uS2

OrganismNot specified

UniProt Q4GWZ2

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain SA； UniProt 1–295	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain SA 1–295（295 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RSSA_PIG
Isoform	—
PDB entities	50
Chains and sequence ranges	Author chain SA； PDBConstruct 1–295； UniProt 1–295

Ribosomal protein eS1

OrganismNot specified

UniProt B6V8C8

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain SB； UniProt 1–264	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain SB 1–264（264 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	B6V8C8_PIG
Isoform	—
PDB entities	51
Chains and sequence ranges	Author chain SB； PDBConstruct 1–264； UniProt 1–264

Ribosomal protein uS3

OrganismNot specified

UniProt Q0Z8U2

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain SD； UniProt 1–243	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain SD 1–243（243 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RS3_PIG
Isoform	—
PDB entities	53
Chains and sequence ranges	Author chain SD； PDBConstruct 1–243； UniProt 1–243

Ribosomal protein eS4

OrganismNot specified

UniProt F1RQ91

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain SE； UniProt 1–263	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence

UniProt name	F1RQ91_PIG
Isoform	—
PDB entities	54
Chains and sequence ranges	Author chain SE； PDBConstruct 1–263； UniProt 1–263

Ribosomal protein uS7

OrganismNot specified

UniProt F2Z5E6

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain SF； UniProt 1–204	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain SF 1–204（204 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	F2Z5E6_PIG
Isoform	—
PDB entities	55
Chains and sequence ranges	Author chain SF； PDBConstruct 1–204； UniProt 1–204

Ribosomal protein eS7

OrganismNot specified

UniProt I3L5B2

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain SH； UniProt 1–194	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence

UniProt name	I3L5B2_PIG
Isoform	—
PDB entities	57
Chains and sequence ranges	Author chain SH； PDBConstruct 1–194； UniProt 1–194

Ribosomal protein eS8

OrganismNot specified

UniProt F2Z5F5

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain SI； UniProt 1–208	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence

UniProt name	F2Z5F5_PIG
Isoform	—
PDB entities	58
Chains and sequence ranges	Author chain SI； PDBConstruct 1–208； UniProt 1–208

Ribosomal protein eS10

OrganismNot specified

UniProt F1RZ28

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain SK； UniProt 1–165	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence

UniProt name	F1RZ28_PIG
Isoform	—
PDB entities	60
Chains and sequence ranges	Author chain SK； PDBConstruct 1–165； UniProt 1–165

Ribosomal protein uS17

OrganismNot specified

UniProt F2Z4Y8

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain SL； UniProt 1–158	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence

UniProt name	F2Z4Y8_PIG
Isoform	—
PDB entities	61
Chains and sequence ranges	Author chain SL； PDBConstruct 1–158； UniProt 1–158

Ribosomal protein uS19

OrganismNot specified

UniProt P62844

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain SP； UniProt 1–145	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain SP 1–145（145 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RS15_PIG
Isoform	—
PDB entities	65
Chains and sequence ranges	Author chain SP； PDBConstruct 1–145； UniProt 1–145

Ribosomal protein uS9

OrganismNot specified

UniProt Q29201

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain SQ； UniProt 1–146	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain SQ 1–146（146 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RS16_PIG
Isoform	—
PDB entities	66
Chains and sequence ranges	Author chain SQ； PDBConstruct 1–146； UniProt 1–146

Ribosomal protein eS17

OrganismNot specified

UniProt Q6QAP7

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain SR； UniProt 1–135	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain SR 1–135（135 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RS17_PIG
Isoform	—
PDB entities	67
Chains and sequence ranges	Author chain SR； PDBConstruct 1–135； UniProt 1–135

Ribosomal protein uS13

OrganismNot specified

UniProt P62272

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain SS； UniProt 1–152	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain SS 1–152（152 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RS18_PIG
Isoform	—
PDB entities	68
Chains and sequence ranges	Author chain SS； PDBConstruct 1–152； UniProt 1–152

Ribosomal protein eS19

OrganismNot specified

UniProt F1RGG1

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain ST； UniProt 1–145	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence

UniProt name	F1RGG1_PIG
Isoform	—
PDB entities	69
Chains and sequence ranges	Author chain ST； PDBConstruct 1–145； UniProt 1–145

Ribosomal protein uS10

OrganismNot specified

UniProt A1XQU9

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain SU； UniProt 1–119	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain SU 1–119（119 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RS20_PIG
Isoform	—
PDB entities	70
Chains and sequence ranges	Author chain SU； PDBConstruct 1–119； UniProt 1–119

Ribosomal protein eS21

OrganismNot specified

UniProt P63221

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain SV； UniProt 1–83	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain SV 1–83（83 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RS21_PIG
Isoform	—
PDB entities	71
Chains and sequence ranges	Author chain SV； PDBConstruct 1–83； UniProt 1–83

Ribosomal protein uS12

OrganismNot specified

UniProt Q6SA96

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain SX； UniProt 1–143	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain SX 1–143（143 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RS23_PIG
Isoform	—
PDB entities	73
Chains and sequence ranges	Author chain SX； PDBConstruct 1–143； UniProt 1–143

Ribosomal protein eS26

OrganismNot specified

UniProt P49171

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain Sa； UniProt 1–115	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain Sa 1–115（115 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RS26_PIG
Isoform	—
PDB entities	76
Chains and sequence ranges	Author chain Sa； PDBConstruct 1–115； UniProt 1–115

Ribosomal protein eS28

OrganismNot specified

UniProt Q6QAT1

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain Sc； UniProt 1–69	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain Sc 1–69（69 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RS28_PIG
Isoform	—
PDB entities	78
Chains and sequence ranges	Author chain Sc； PDBConstruct 1–69； UniProt 1–69

Ribosomal protein uS14

OrganismNot specified

UniProt Q6QAP6

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain Sd； UniProt 1–56	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain Sd 1–56（56 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RS29_PIG
Isoform	—
PDB entities	79
Chains and sequence ranges	Author chain Sd； PDBConstruct 1–56； UniProt 1–56

Ribosomal protein eS31

OrganismNot specified

UniProt F2Z5U0

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain Sf； UniProt 1–156	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein RACK1 × 1 （P63246） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence

UniProt name	F2Z5U0_PIG
Isoform	—
PDB entities	81
Chains and sequence ranges	Author chain Sf； PDBConstruct 1–156； UniProt 1–156

Ribosomal protein RACK1

OrganismNot specified

UniProt P63246

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 78 RNA 4 PDB declaration: 82-meric（82） Consistent with all polymer counts	Chain Sg； UniProt 1–317	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （F2Z567） Ribosomal protein uL3 × 1 Ribosomal protein uL4 × 1 Ribosomal protein uL18 × 1 （F1S530） Ribosomal protein eL6 × 1 Ribosomal protein uL30 × 1 Ribosomal protein eL8 × 1 （I3LVI8） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein uL11 × 1 （Q6QAS5） Ribosomal protein eL13 × 1 （I3LSD3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （I3LK33） Ribosomal protein uL13 × 1 （F1RI01） Ribosomal protein uL22 × 1 Ribosomal protein eL18 × 1 （I3L6F1） Ribosomal protein eL19 × 1 （F2Z546） Ribosomal protein eL20 × 1 （F1S935） Ribosomal protein eL21 × 1 （F1RTJ9） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （P62831） Ribosomal protein eL24 × 1 Ribosomal protein uL23 × 1 （F2Z522） Ribosomal protein uL24 × 1 （I3L7Y1） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （F1RGC0） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （F2Z554） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （F2Z5Q8） Ribosomal protein eL34 × 1 （F2Z5B9） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （F2Z5K6） Ribosomal protein eL37 × 1 Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （K7GP63） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （P83884） Ribosomal protein eL43 × 1 Ribosomal protein uL10 × 1 Ribosomal protein eL28 × 1 Eukaryotic elongation factor 2 × 1 18S ribosomal RNA × 1 Ribosomal protein uS2 × 1 （Q4GWZ2） Ribosomal protein eS1 × 1 （B6V8C8） Ribosomal protein uS5 × 1 Ribosomal protein uS3 × 1 （Q0Z8U2） Ribosomal protein eS4 × 1 （F1RQ91） Ribosomal protein uS7 × 1 （F2Z5E6） Ribosomal protein eS6 × 1 Ribosomal protein eS7 × 1 （I3L5B2） Ribosomal protein eS8 × 1 （F2Z5F5） Ribosomal protein uS4 × 1 Ribosomal protein eS10 × 1 （F1RZ28） Ribosomal protein uS17 × 1 （F2Z4Y8） Ribosomal protein eS12 × 1 Ribosomal protein uS15 × 1 Ribosomal protein uS11 × 1 Ribosomal protein uS19 × 1 （P62844） Ribosomal protein uS9 × 1 （Q29201） Ribosomal protein eS17 × 1 （Q6QAP7） Ribosomal protein uS13 × 1 （P62272） Ribosomal protein eS19 × 1 （F1RGG1） Ribosomal protein uS10 × 1 （A1XQU9） Ribosomal protein eS21 × 1 （P63221） Ribosomal protein uS8 × 1 Ribosomal protein uS12 × 1 （Q6SA96） Ribosomal protein eS24 × 1 Ribosomal protein es25 × 1 Ribosomal protein eS26 × 1 （P49171） Ribosomal protein eS27 × 1 Ribosomal protein eS28 × 1 （Q6QAT1） Ribosomal protein uS14 × 1 （Q6QAP6） Ribosomal protein eS30 × 1 Ribosomal protein eS31 × 1 （F2Z5U0） MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 3J7P	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain Sg 1–317（317 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	GBLP_PIG
Isoform	—
PDB entities	82
Chains and sequence ranges	Author chain Sg； PDBConstruct 1–317； UniProt 1–317

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

Download Download SAXS (.dat) P(r) (.pr.out) CIF

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	3j7p
Deposition date deposition_date	2014-08-01
Structure title title	Structure of the 80S mammalian ribosome bound to eEF2
Keywords keywords	mammalian, Sec61, translocation, translation, eEF2, RIBOSOME; RIBOSOME
Experimental Method method	ELECTRON MICROSCOPY

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	94.29 Å
Radius of gyration Rg (electron density) rg_electron	95.20 Å
Forward intensity I(0) i0	298668000000.00
Molecular weight molecular_weight	3270300.0 kDa
Excluded volume excluded_volume	3516600 Å³
Envelope volume envelope_volume	5841500 Å³
Hydration-shell volume shell_volume	463360 Å³
Envelope diameter envelope_diameter	316.5 Å
Shell Rg shell_rg	112.60 Å
Envelope Rg envelope_rg	94.07 Å
Shape Rg shape_rg	95.25 Å
Total Rg total_rg	95.13 Å
Total atoms total_atoms	221685
Residues n_residues	18180
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	242.5 Å
Rg (real space) rg_real	91.66 Å
Rg uncertainty (real space) rg_real_error	0.33 Å
I(0) (real space) i0_real	2.8640e+11
I(0) uncertainty (real space) i0_real_error	4.6120e+09
Rg (reciprocal space) rg_reciprocal	96.10 Å
I(0) (reciprocal space) i0_reciprocal	300400000000.0000
Solution quality estimate total_estimate	0.9120
Solution quality rating solution_quality	EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks	1
Primary peak position r_peak_primary	106.1 Å
Skewness Skewness skewness	0.096
Kurtosis Kurtosis kurtosis	-0.610
Angular range angular_range	— – 0.0800 Å⁻¹
Current regularization parameter α current_alpha	0.8521
Highest regularization parameter α highest_alpha	15580000000.0000
Real-space data points n_real_points	17
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 1.000; Stabil: 0.955; Sysdev: 1.000; Positv: 1.000; Valcen: 0.993; Smooth: 0.000

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (84)

Entity 1 Entity 1 polymer

Description description	28S ribosomal RNA
Molecular weight formula_weight	1187230.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	5
Atom count atom_count	221686

CGCGACCUCAGAUCAGACGUGGCGACCCGCUGAAUUUAAGCAUAUUAGUCAGCGGAGGAGAAGAAACUAACCAGGAUUCCCUCAGUAACGGCGAGUGAACAGGGAAGAGCCCAGCGCCGAAUCCCCGCCCCGCGGCGGGGCGCGGGACAUGUGGCGUACGGAAGACCCGCUCCCCGGCGCCGCUCGUGGGGGGCCCAAGUCCUUCUGAUCGAGGCCCAGCCCGUGGACGGUGUGAGGCCGGUAGCGGCCCCCGGCGCGCCGGGCCCGGGUCUUCCCGGAGUCGGGUUGCUUGGGAAUGCAGCCCAAAGCGGGUGGUAAACUCCAUCUAAGGCUAAAUACCGGCACGAGACCGAUAGUCAACAAGUACCGUAAGGGAAAGUUGAAAAGAACUUUGAAGAGAGAGUUCAAGAGGGCGUGAAACCGUUAAGAGGUAAACGGGUGGGGUCCGCGCAGUCCGCCCGGAGGAUUCAACCCGGCGGCGGGUCCGGCCGUGUCGGCGGCCCGGCGGAUCUUUCCCGCGCGGGGGACCGUCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCAUUUCCACCGCGGCGGUGCGCCGCGACCGGCUCCGGGACGGCUGGGAAGGCCCGGCGGGGAAGGUGGCUCGGCCGAGUGUUACAGCCCCCCCGGCAGCAGCACUCGCCGAAUCCCGGGGCCGAGGGAGCGAGACCCGUCGCCGCGCUCUCCCCCCUCCCGGCGCCGCCGGGGGGGGCCGGGCCACCCCUCCCACGGCGCGACCGCUCGGGGCGGACUGUCCCCAGUGCGCCCCGGGCGGGUCGCGCCGUCGGGCCCGGGGGAGGGGCCACGCGCGCGUCCCCCGAAGAGGGGGACGGCGGAGCGAGCGCACGGGGUCGGCGGCGACGUCGGCUACCCACCCGACCCGUCUUGAAACACGGACCAAGGAGUCUAACACGUGCGCGAGUCGGGGGCUCGCACGAAAGCCGCCGUGGCGCAAUGAAGGUGAAGGCCGGCGCGCUCGCCGGCCGAGGUGGGAUCCCGAGGCCUCUCCAGUCCGCCGAGGGCGCACCACCGGCCCGUCUCGCCCGCCGCGCCGGGGAGGUGGAGCACGAGCGCACGUGUUAGGACCCGAAAGAUGGUGAACUAUGCCUGGGCAGGGCGAAGCCAGAGGAAACUCUGGUGGAGGUCCGUAGCGGUCCUGACGUGCAAAUCGGUCGUCCGACCUGGGUAUAGGGGCGAAAGACUAAUCGAACCAUCUAGUAGCUGGUUCCCUCCGAAGUUUCCCUCAGGAUAGCUGGCGCUCUCGCACACGCAGUUUUAUCCGGUAAAGCGAAUGAUUAGAGGUCUUGGGGCCGAAACGAUCUCAACCUAUUCUCAAACUUUAAAUGGGUAAGAAGCCCGGCUCGCUGGCGUGGAGCCGGGCGUGGAAUGCGAGUGCCUAGUGGGCCACUUUUGGUAAGCAGAACUGGCGCUGCGGGAUGAACCGAACGCCGGGUUAAGGCGCCCGAUGCCGACGCUCAUCAGACCCCAGAAAAGGUGUUGGUUGAUAUAGACAGCAGGACGGUGGCCAUGGAAGUCGGAAUCCGCUAAGGAGUGUGUAACAACUCACCUGCCGAAUCAACUAGCCCUGAAAAUGGAUGGCGCUGGAGCGUCGGGCCCAUACCCGGCCGUCGCCGGCAGUCGAGAGUGGACGGGAGCGGCGGGGGCGGCGCGCGCGCGCGCCCGCCCCCGGAGCCCCGCGGACGCUACGCCGCGACGAGUAGGAGGGCCGCUGCGGUGAGCCUUGAAGCCUAGGGCGCGGGCCCGGGUGGAGCCGCCGCAGGUGCAGAUCUUGGUGGUAGUAGCAAAUAUUCAAACGAGAACUUUGAAGGCCGAAGUGGAGAAGGGUUCCAUGUGAACAGCAGUUGAACAUGGGUCAGUCGGUCCUGAGAGAUGGGCGAGCGCCGUUCCGAAGGGACGGGCGAUGGCCUCCGUUGCCCUCGGCCGAUCGAAAGGGAGUCGGGUUCAGAUCCCCGAAUCCGGAGUGGCGGAGAUGGGCGCCGCGAGGCGUCCAGUGCGGUAACGCGACCGAUCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGUUCUCUUUUCUUUGUGAAGGGCAGGGCGCCCUGGAAUGGGUUCGCCCCGAGAGAGGGGCCCGUGCCUUGGAAAGCGUCGCGGUUCCGGCGGCGUCCGGUGAGCUCUCGCUGGCCCUUGAAAAUCCGGGGGAGAGGGUGUAAAUCUCGCGCCGGGCCGUACCCAUAUCCGCAGCAGGUCUCCAAGGUGAACAGCCUCUGGCAUGUUGGAACAAUGUAGGUAAGGGAAGUCGGCAAGCCGGAUCCGUAACUUCGGGAUAAGGAUUGGCUCUAAGGGCUGGGUCGGUCGUCGGCCGGCGCCUAGCAGCCGACUUAGAACUGGUGCGGACCAGGGGAAUCCGACUGUUUAAUUAAAACAAAGCAUCGCGAAGGCCCGCGGCGGGUGUUGACGCGAUGUGAUUUCUGCCCAGUGCUCUGAAUGUCAAAGUGAAGAAAUUCAAUGAAGCGCGGGUAAACGGCGGGAGUAACUAUGACUCUCUUAAGGUAGCCAAAUGCCUCGUCAUCUAAUUAGUGACGCGCAUGAAUGGAUGAACGAGAUUCCCACUGUCCCUACCUACUAUCCAGCGAAACCACAGCCAAGGGAACGGGCUUGGCGGAAUCAGCGGGGAAAGAAGACCCUGUUGAGCUUGACUCUAGUCUGGCACGGUGAAGAGACAUGUUUUUUCACUGACCCGGUGAGGCGGGGGGGCGAGCCCCGAGGGGCUCUCGCUUCUGGCGCCAAGCGCCCGGCCGCGCGCCGGCCGGGCGCGACCCGCUCCGGGGACAGUGCCAGGUGGGGAGUUUGACUGGGGCGGUACACCUGUCAAACGGUAACGCAGGUGUCCUAAGGCGAGCUCAGGGAGGACAGAAACCUCCCGUGGAGCAGAAGGGCAAAAGCUCGCUUGAUCUUGAUUUUCAGUACGAAUACAGACCGUGAAAGCGGGGCCUCACGAUCCUUCUGACCUUUUGGGUUUUAAGCAGGAGGUGUCAGAAAAGUUACCACAGGGAUAACUGGCUUGUGGCGGCCAAGCGUUCAUAGCGACGUCGCUUUUUGAUCCUUCGAUGUCGGCUCUUCCUAUCAUUGUGAAGCAGAAUUCACCAAGCGUUGGAUUGUUCACCCACUAAUAGGGAACGUGAGCUGGGUUUAGACCGUCGUGAGACAGGUUAGUUUUACCCUACUGAUGAUGUGUUGUUGCCAUGGUAAUCCUGCUCAGUACGAGAGGAACCGCAGGUUCAGACAUUUGGUGUAUGUGCUUGGCUGAGGAGCCAAUGGGGCGAAGCUACCAUCUGUGGGAUUAUGACUGAACGCCUCUAAGUCAGAAUCCCGCCCAGGCGGAACGAUACGGCAGCGCCGCGGAGCCUCGGUUGGCCUCGGAUAGCCGGUCCCCCGCGGGGUCCGGUGCGGAGUGCCCUUCGUCCUGGGAAACGGGGCGCGGCCGGAGAGGCGGCCGCCCCCUCGCCCGUCACGCACGCCGUUCGUGGGGAACCUGGCGCUAAACCAUUCGUAGACGACCUGCUUCUGGGUCGGGGUUUCGUACGUAGCAGAGCAGCUCCCUCGCUGCGAUCUAUUGAAAGUCAGCCCUCGACACAAGGGUUUGU

Entity 2 Entity 2 polymer

Description description	5S ribosomal RNA
Molecular weight formula_weight	38691.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	7

GUCUACGGCCAUACCACCCUGAACGCGCCCGAUCUCGUCUGAUCUCGGAAGCUAAGCAGGGUCGGGCCUGGUUAGUACUUGGAUGGGAGACCGCCUGGGAAUACCGGGUGCUGUAGGCUU

Entity 3 Entity 3 polymer

Description description	5.8S ribosomal RNA
Molecular weight formula_weight	50143.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	8

CGACUCUUAGCGGUGGAUCACUCGGCUCGUGCGUCGAUGAAGAACGCAGCUAGCUGCGAGAAUUAAUGUGAAUUGCAGGACACAUUGAUCAUCGACACUUCGAACGCACUUGCGGCCCCGGGUUCCUCCCGGGGCUACGCCUGUCUGAGCGUCGCU

Entity 4 Entity 4 polymer

Description description	Ribosomal protein uL2
Molecular weight formula_weight	28088.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A

MGRVIRGQRKGAGSVFRAHVKHRKGAARLRAVDFAERHGYIKGIVKDIIHDPGRGAPLAKVVFRDPYRFKKRTELFIAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISSANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQEKEN

Entity 5 Entity 5 polymer

Description description	Ribosomal protein uL3
Molecular weight formula_weight	44759.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

SHRKFSAPRHGSLGFLPRKRSSRHRGKVKSFPKDDSSKPVHLTAFLGYKAGMTHIVREVDRPGSKVNKKEVVEAVTIVETPPMIVVGIVGYVETPRGLRTFKTIFAEHISDECKRRFYKNWHKSKKKAFTKYCKKWQDAAGAAALAADFSSMKAYCQVIRVIAHTQMRLLPLRQKKAHLMEIQVNGGTVAEKLDWARERLEQQVPVNQVFGQDEMIDVIGVTKGKGYKGVTSRWHTKKLPRKTHRGLRKVACIGAWHPARVAFSVARAGQKGYHHRTEINKKIYKIGQGYLIKDGKLIKNNASTDYDLSDKSINPLGGFVHYGEVTNDFVMLKGCVVGTKKRVLTLRKSLLVQTKRRALEKIDLKFIDTTSKFGHGRFQTVEEKKAFMGPLKKD

Entity 6 Entity 6 polymer

Description description	Ribosomal protein uL4
Molecular weight formula_weight	41635.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C

ACARPLISVYSEKGESSGKNVTLPAVFKAPIRPDIVNFVHTNLRKNNRQPYAVSELAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTWRRWHRRVNTTQKRYAICSALAASALPALVMSKGHRIEEVPELPLVVEDKVESYKKTKEAVLLLKKLKAWNDIKKVYASQRMRAGKGKMRNRRRIQRRGPCIIYNEDNGIIKAFRNIPGITLLNVSKLNILKLAPGGHVGRFCIWTESAFRKLDELYGTWRKAATLKSNYNLPMHKMLNTDLSRILKSPEIQRALRAPRKKIHRRVLKKNPLKNLRIMLKLNPYAKTMRRNTILRQARNHKIRMDKAAAAAAALKAKSGEK

Entity 7 Entity 7 polymer

Description description	Ribosomal protein uL18
Molecular weight formula_weight	34417.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D

MGFVKVVKNKAYFKRYQVKFRRRREGKTDYYARKRLVIQDKNKYNTPKYRMIVRVTNRDIICQIAYARIEGDMIVCAAYAHELPKYGVKVGLTNYAAAYCTGLLLARRLLNRFGMDKIYEGQVEVTGDEYNVESIDGQPGAFTCYLDAGLARTTTGNKVFGALKGAVDGGLSIPHSTKRFPGYDSESKEFNAEVHRKHILGQNVADYMRYLIEEDEDAYKKQFSQYIKNNVTPDMMEEMYKKAHAAIRENPVYEKKPKKEVKKKRWNRPKMSLAQKKDRVAQKKASFLRAQERAAES

Entity 8 Entity 8 polymer

Description description	Ribosomal protein eL6
Molecular weight formula_weight	27052.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

PHCSRNPVLVRGIGRYSRSAMYSRMAMYKRKYSAAVLATVTKPVGGDKNGGTRVVKLRKMPRYYPTEDVPRKLLSHGKKPFSKHVRKLRASITPGTILIILTGRHRGKRVIFLKQLSSGLLLVTGPLVLNRVPLRRTHQKFVIATSTKIDISGVKIPKHLTDAYFKKKKLRKPRHQEGEIFDTEKEKYEISEQRKVDQKAVDSQILRRIKAVPQLQGYLRSVFALTNGIYPHKLVF

Entity 9 Entity 9 polymer

Description description	Ribosomal protein uL30
Molecular weight formula_weight	26590.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

NFAELKIKRLRKKFAQKMLRKARRKLIYEKAKHYHKEYRQMYRTEIRMARMARKAGNFYVPAEPKLAFVIRIRGINGVSPKVRKVLQLLRLRQIFNGTFVKLNKASINMLRIVEPYIAWGYPNLKSVNELIYKRGYGKINKKRIALTDNALIARSLGKYGIICMEDLIHEIYTVGKRFKEANNFLWPFKLSSPRGGMKKKTTHFVEGGDAGNREDQINRLIRRMN

Entity 10 Entity 10 polymer

Description description	Ribosomal protein eL8
Molecular weight formula_weight	30166.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G

MPKGKKAKGKKVAPAPAVVKKQEAKKVVNLLFEKRPKNFGIGQDIQPKRDLTRFVKWPRYIRLQRQRAILYKRLKVPPTINQFTQALDRQTATQLLKLTHKYRPETKQEKKQRLLARAEKKAAGKGDVPTKRPPVLRAGVNTVTTLVENKKAQLVVIAHDVDPIELVVFLPALCRKMGVPYCIIKGKARLGRLVHRKTCTTVAFTQVNSEDKGALAKLVEAIRTNYNDRYNEIRRHWGGNVLGPKSVARITKLEKAKAKELATKLG

Entity 11 Entity 11 polymer

Description description	Ribosomal protein uL6
Molecular weight formula_weight	21899.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H

MKTILSNQTVDIPENVDITLKGRTVIVKGPRGTLRRDFNHINVELSLLGKKKKRLRVDKWWGNRKELATVRTICSHVQNMIKGVTLGFRYKMRSVYAHFPINVVIQENGSLVEIRNFLGEKYIRRVRMRPGVACSVSQAQKDELILEGNDIELVSNSAALIQQATTVKNKDIRKFLDGIYVSEKGTVQQADE

Entity 12 Entity 12 polymer

Description description	Ribosomal protein uL16
Molecular weight formula_weight	24465.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I

GRRPARCYRYCKNKPYPKSRFCRGVPDAKIRIFDLGRKKAKVDEFPLGGHMVSDEYEQLSSEALEAARICANKYMVKSCGRDGFHMRVRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVHIGQVIMSIRTKLQNEEHVIEALRRAKFKFPGRQKIHISKKWGFTKFNADEFENMVAKKCLIPDGCGVKYVPNHGPLDKWRVLHS

Entity 13 Entity 13 polymer

Description description	Ribosomal protein uL5
Molecular weight formula_weight	20248.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J

MAQDQGEKENPMRELRIRKLCLNICVGESGDRLTRAAKVLEQLTGQTPVFSKARYTVRSFGIRRNEKIAVHCTVRGAKAEEILEKGLKVREYELRKNNFSDTGNFGFGIQEHIDLGIKYDPSIGIYGLDFYVVLGRPGFSIADKKRRTGCIGAKHRISKEEAMRWFQQKHDGIILPGN

Entity 14 Entity 14 polymer

Description description	Ribosomal protein uL11
Molecular weight formula_weight	17689.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K

MPPKFDPNEIKVVYLRCTGGEVGATSALAPKIGPLGLSPKKVGDDIAKATGDWKGLRITVKLTIQNRQAQIEVVPSASALIIKALKEPPRDRKKQKNIKHSGNITFDEIVNIARQMRHRSLARELSGTIKEILGTAQSVGCNVDGRHPHDIIDDINSGAVECP

Entity 15 Entity 15 polymer

Description description	Ribosomal protein eL13
Molecular weight formula_weight	24365.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	L

MAPSRNGMILKPHFHKDWQRRVATWFNQPARKIRRRKARQAKARRIAPRPASGPLRPVVRCPTVRYHTKVRAGRGFSLEELRVAGIHKKVARTIGISVDPRRRNKCTESLQANVQRLKEYRSKLILFPRKPSAPKKGDSSAEELKLATQLTGPVMPIRNVYKREKARVITDEEKNFKAFASLRMARANARLFGIRAKRAKEAAEQDVEKKK

Entity 16 Entity 16 polymer

Description description	Ribosomal protein eL14
Molecular weight formula_weight	23383.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	M

MVFRRFVEVGRVAYVSFGPHAGKLVAIVDVIDQNRALVDGPCTQVRRQAMPFKCMQLTDFILKFPHSARQKYVRKAWEKADINAKWAATRWAKKIEAREKKAKMTDFDRYKVMKARKMRNRLIKLEVKKLQKAALLKASPKKALAKGAAAAAAAAAAKVPVKKITTAGKKAPAQKAPAQKAAGQKAAPPPKAQKVQKPPAQKAPAPKASGEKA

Entity 17 Entity 17 polymer

Description description	Ribosomal protein eL15
Molecular weight formula_weight	24207.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	N

MGAYKYIQELWRKKQSDVMRFLLRVRCWQYRQLSALHRAPRPTRPDKARRLGYKAKQGYVIYRIRVRRGGRKRPVPKGATYGKPVHHGVNQLKFARSLQSVAEERAGRHCGALRVLNSYWVGEDSTYKFFEVILIDPFHKAIRRNPDTQWITKPVHKHREMRGLTSAGRKSRGLGKGHKFHHTIGGSRRAAWRRRNTLQLHRYR

Entity 18 Entity 18 polymer

Description description	Ribosomal protein uL13
Molecular weight formula_weight	23765.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	O

MAEGQQVLVLDGRGHLLGRLAAIVAKQVLLGRKVVVVRCEGINISGNFYRNKLKYLAFLRKRMNTNPSRGPYHFRAPSRIFWRTVRGMLPHKTKRGQAALDRLKVFDGIPPPYDKKKRMVVPAALKVVRLKPTRKFAYLGRLAHEVGWKYQAVTATLEEKRKEKAKIHYRKKKQLMRLRKQAEKNVEKKIDRFTEVLKTHGFLV

Entity 19 Entity 19 polymer

Description description	Ribosomal protein uL22
Molecular weight formula_weight	17757.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	P

VRYSLDPENPTKSCKSRGSNLRVHFKNTRETAQAIKGMHIRKATKYLKDVTLQKQCVPFRRYNGGVGRCAQAKQWGWTQGRWPKKSAEFLLHMLKNAESNAELKGLDVDSLVIEHIQVNKAPKMRRRTYRAHGRINPYMSSPCHIEMILTEKE

Entity 20 Entity 20 polymer

Description description	Ribosomal protein eL18
Molecular weight formula_weight	21588.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Q

MGVDIRHNKDRKVRRKEPKSQDIYLRLLVKLYRFLARRTNSTFNQVVLKRLFMSRTNRPPLSLSRMIRKMKLPGREGKTAVVVGTITDDVRVQEVPKLKVCALRVSSRARSRILKAGGKILTFDQLALDSPKGCGTVLLSGPRKGREVYRHFGKAPGTPHSHTKPYVRSKGRKFERARGRRASRGYKN

Entity 21 Entity 21 polymer

Description description	Ribosomal protein eL19
Molecular weight formula_weight	23567.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	R

YSMLRLQKRLASSVLRCGKKKVWLDPNETNEIANANSRQQIRKLIKDGLIIRKPVTVHSRARCRKNTLARRKGRHMGIGKRKGTANARMPEKVTWMRRMRILRRLLRRYRESKKIDRHMYHSLYLKVKGNVFKNKRILMEHIHKLKADKARKKLLADQAEARRSKTKEARKRREERLQAKKEEIIKTLSKEEETKK

Entity 22 Entity 22 polymer

Description description	Ribosomal protein eL20
Molecular weight formula_weight	26103.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	S

MKKQIWGRGTNGLPASKGRDFRQRNQGERLRESGYFLSGGGGERGESAMKASGTLREYKVVGRCLPTPKCRTPPLYRMRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAASKCRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF

Entity 23 Entity 23 polymer

Description description	Ribosomal protein eL21
Molecular weight formula_weight	18610.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	T

MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA

Entity 24 Entity 24 polymer

Description description	Ribosomal protein eL22
Molecular weight formula_weight	14785.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	U

MAPVKKLVAKGGKKKKQVLKFTLDCTHPVEDGIMDAANFEQFLQERIKVNGKAGNLGGGVVTIERSKSKITVTSEVPFSKRYLKYLTKKYLKKNNLRDWLRVVANSKESYELRYFQINQDEEEEEDED

Entity 25 Entity 25 polymer

Description description	Ribosomal protein uL14
Molecular weight formula_weight	14892.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	V

MSKRGRGGSSGAKFRISLGLPVGAVINCADNTGAKNLYIISVKGIKGRLNRLPAAGVGDMVMATVKKGKPELRKKVHPAVVIRQRKSYRRKDGVFLYFEDNAGVIVNNKGEMKGSAITGPVAKECADLWPRIASNAGSIA

Entity 26 Entity 26 polymer

Description description	Ribosomal protein eL24
Molecular weight formula_weight	17825.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	W

MKVELCSFSGYKIYPGHGRRYARTDGKVFQFLNAKCESAFLSKRNPRQINWTVLYRRKHKKGQSEEIQKKRTRRAVKFQRAITGASLADIMAKRNQKPEVRKAQREQAIRAAKEAKKAKQASKKTAMAAAKAPTKAAPKQKIVKPVKVSAPRVGGKR

Entity 27 Entity 27 polymer

Description description	Ribosomal protein uL23
Molecular weight formula_weight	17740.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	X

MAPKAKKEAPAPPKAEAKAKALKAKKAVLKGVHSHKKKKIRTSPTFRRPKTLRLRRQPKYPRKSAPRRNKLDHYAIIKFPLTTESAMKKIEDNNTLVFIVDVKANKHQIKQAVKKLYDIDVAKVNTLIRPDGEKKAYVRLAPDYDALDVANKIGII

Entity 28 Entity 28 polymer

Description description	Ribosomal protein uL24
Molecular weight formula_weight	17303.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Y

MKFNPFVTSDRSKNRKRHFNAPSHIRRKIMSSPLSKELRQKYNVRSMPIRKDDEVQVVRGHYKGQQIGKVVQVYRKKYVIYIERVQREKANGTTVHVGIHPSKVVITRLKLDKDRKKILERKAKSRQVGKEKGKYKEETIEKMQE

Entity 29 Entity 29 polymer

Description description	Ribosomal protein eL27
Molecular weight formula_weight	15835.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Z

MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF

Entity 30 Entity 30 polymer

Description description	Ribosomal protein uL15
Molecular weight formula_weight	16631.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	a

QPSRLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHLKRNQSFCPTVNLDKLWTLVSEQTRINAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKGVGGACVLVA

Entity 31 Entity 31 polymer

Description description	Ribosomal protein eL29
Molecular weight formula_weight	17545.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	b

MAKSKNHTTHNQSRKWHRNGIKKPRSQRYESLKGVDPKFLRNMRFAKKHNKKGLKKMQANNAKAMSARAEAIKALVKPKEVKPNIPKGGSRKLSRLAYIAHPKLGKRARARIAKGLRFCRPKSQAKAQSKAKATAGGTAAAPVPPASAPKGAQAPTKAPQ

Entity 32 Entity 32 polymer

Description description	Ribosomal protein eL30
Molecular weight formula_weight	12805.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	c

MVAAKKTKKSLESINSRLQLVMKSGKYVLGYKQTLKMIRQGKAKLVILANNCPALRKSEIEYYAMLAKTGVHHYSGNNIELGTACGKYYRVCTLAIIDPGDSDIIRSMPEQTGEK

Entity 33 Entity 33 polymer

Description description	Ribosomal protein eL31
Molecular weight formula_weight	14494.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	d

MAPAKKGGEKKKGRSAINEVVTREYTINIHKRIHGVGFKKRAPRALKEIRKFAMKEMGTPDVRIDTRLNKAVWAKGIRNVPYRIRVRLSRKRNEDEDSPNKLYTLVTYVPVTTFKNLQTVNVDEN

Entity 34 Entity 34 polymer

Description description	Ribosomal protein eL32
Molecular weight formula_weight	15898.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	e

MAALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRVRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAHNVSSKNRKAIVERAAQLAIRVTNPNARLRSEENE

Entity 35 Entity 35 polymer

Description description	Ribosomal protein eL33
Molecular weight formula_weight	12564.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	f

MSGRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPGGKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI

Entity 36 Entity 36 polymer

Description description	Ribosomal protein eL34
Molecular weight formula_weight	13326.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	g

MVQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLKAQAQSQKAK

Entity 37 Entity 37 polymer

Description description	Ribosomal protein uL29
Molecular weight formula_weight	14591.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	h

MAKIKARDLRGKKKEELLKQLEDLKVELSQLRVAKVTGGAASKLSKIRVVRKSIARVLTVINQTQKENLRKFYKGKKYKPLDLRPKKTRAMRRRLNKHEENLKTKKQQRKERLYPLRKFAVKA

Entity 38 Entity 38 polymer

Description description	Ribosomal protein eL36
Molecular weight formula_weight	12290.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	i

MALRYPMAVGLNKGHKVTKNVSKPRHSRRRGRLTKHTKFVRDMIREVCGFAPYERRAMELLKVSKDKRALKFIKKRVGTHIRAKRKREELSNVLAAMRKAAAKKD

Entity 39 Entity 39 polymer

Description description	Ribosomal protein eL37
Molecular weight formula_weight	10102.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	j

TKGTSSFGKRRNKTHTLCRRCGSKAYHLQKSTCGKCGYPAKRKRKYNWSAKAKRRNTTGTGRMRHLKIVYRRFRHGFREGITPKPK

Entity 40 Entity 40 polymer

Description description	Ribosomal protein eL38
Molecular weight formula_weight	8238.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	k

MPRKIEEIKDFLLTARRKDAKSVKIKKNKDNVKFKVRCSRYLYTLVITDKEKAEKLKQSLPPGLAVKELK

Entity 41 Entity 41 polymer

Description description	Ribosomal protein eL39
Molecular weight formula_weight	6426.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	l

MSSHKTFRIKRFLAKKQKQNRPIPQWIRMKTGNKIRYNSKRRHWRRTKLGL

Entity 42 Entity 42 polymer

Description description	Ribosomal protein eL40
Molecular weight formula_weight	14758.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	m

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGIIEPSLRQLAQKYNCDKMICRKCYARLHPRAVNCRKKKCGHTNNLRPKKKVK

Entity 43 Entity 43 polymer

Description description	Ribosomal protein eL41
Molecular weight formula_weight	3473.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	n

MRAKWRKKRMRRLKRKRRKMRQRSK

Entity 44 Entity 44 polymer

Description description	Ribosomal protein eL42
Molecular weight formula_weight	12477.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	o

MVNVPKTRRTFCKKCGKHQPHKVTQYKKGKDSLYAQGKRRYDRKQSGYGGQTKPIFRKKAKTTKKIVLRLECVEPNCRSKRMLAIKRCKHFELGGDKKRKGQVIQF

Entity 45 Entity 45 polymer

Description description	Ribosomal protein eL43
Molecular weight formula_weight	10168.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	p

AKRTKKVGIVGKYGTRYGASLRKMVKKIEISQHAKYTCSFCGKTKMKRRAVGIWHCGSCMKTVAGGAWTYNTTSAVTVKSAIRRLKELKDQ

Entity 46 Entity 46 polymer

Description description	Ribosomal protein uL10
Molecular weight formula_weight	22217.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	q

DRATWKSNYFLKIIQLLDDYPKCFIVGADNVGSKQMQQIRMSLRGKAVVLMGKNTMMRKAIRGHLENNPALEKLLPHIRGNVGFVFTKEDLTEIRDMLRANKVPAAARAGAIAPCEVTVPAQNTGLGPEKTSFFQALGITTKISRGTIEILSDVQLIKTGDKVGASEATLLNMLNISPFSFGLVIQQVFDNGSIYNPEVLDI

Entity 47 Entity 47 polymer

Description description	Ribosomal protein eL28
Molecular weight formula_weight	14301.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	r

SAHLQWMVVRNCSSFLIKRNKQTYSTEPNNLKARNSFRYNGLIHHKTVGVEPAADGKGVVVVIKRRSGQRKPATSYVRTTINKNARATLSSIRHMIRKNKYRPDLRMAAIRRASAILRSQKPVMV

Entity 48 Entity 48 polymer

Description description	Eukaryotic elongation factor 2
Molecular weight formula_weight	95232.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	4

NFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLNFIKQSKDGAGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEGQLGPAERAKKVEDMMKKLWGDRYFDPANGKFSKSATSPEGKKLPRTFCQLILDPIFKVFDAIMNFKKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGLVSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL

Entity 49 Entity 49 polymer

Description description	18S ribosomal RNA
Molecular weight formula_weight	561958.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	S2

UACCUGGUUGAUCCUGCCAGUAGCAUAUGCUUGUCUCAAAGAUUAAGCCAUGCAUGUCUAAGUACGCACGGCCGGUACAGUGAAACUGCGAAUGGCUCAUUAAAUCAGUUAUGGUUCCUUUGGUCGCUCGCUCCUCUCCCACUUGGAUAACUGUGGUAAUUCUAGAGCUAAUACAUGCCGACGGGCGCUGACCCCCUUCGCGGGGGGGAUGCGUGCAUUUAUCAGUGGUGACUCUAGAUAACCUCGGGCCGAUCGCACGCCCCCCGUGGCGGCGACGACCCAUUCGAACGUCUGCCCUAUCAACUUUCGAUGGUAGUCGCCGUGCCUACCAUGGUGACCACGGGUGACGGGGAAUCAGGGUUCGAUUCCGGAGAGGGAGCCUGAGAAACGGCUACCACAUCCAAGGAAGGCAGCAGGCGCGCAAAUUACCCACUCCCGACCCGGGGAGGUAGUGACGAAAAAUAACAAUACAGGACUCUUUCGAGGCCCUGUAAUUGGAAUGAGUCCACUUUAAAUCCUUCCGCGAGGAUCCAUUGGAGGGCAAGUCUGGUGCCAGCAGCCGCGGUAAUUCCAGCUCCAAUAGCGUAUAUUAAAGUUGCUGCAGUUAAAAAGCUCGUAGUUGGAUCUUGGGAGCGGGCGUCCCCGCCGCCUCUCGGCCGGGGCCCGAAGCAUUUACUUUGAAAAAAUUAGAGUGUUCAAAGCAGGCCCGAGCCGCCUGGAUACCGCAGCUAGGAAUAAUGGAAUAGGACCGCGGUUCUAUUUUGUUGGUUUUCGGAACUGAGGCCAUGAUUAAGAGGGACGGCCGGGGGCAUUCGUAUUGCGCCGCUAGAGGUGAAAUUCUUGGACCGGCGCAAGACGGACCAGAGCGAAAGCAUUUGCCAAGAAUGUUUUCAUUAAUCAAGAACGAAAGUCGGAGGUUCGAAGACGAUCAGAUACCGUCGUAGUUCCGACCAUAAACGAUGCCGACUGGCGAUGCGGCGGCGUUAUUCCCAUGACCCGCCGGGCAGCUUCCGGGAAACCAAAGUCUUUGGGUUCCGGGGGGAGUAUGGUUGCAAAGCUGAAACUUAAAGGAAUUGACGGAAGGGCACCACCAGGAGUGGAGCCUGCGGCUUAAUUUGACUCAACACGGGAAACCUCACCCGGCCCGGACACGGACAGGAUUGACAGAUUGAUAGCUCUUUCUCGAUUCCGUGGGUGGUGGUGCAUGGCCGUUCUUAGUUGGUGGAGCGAUUUGUCUGGUUAAUUCCGAUAACGAACGAGACUCUGGCAUGCUAACUAGUUACGCGACCCCCGAGCGGUCGGCGUCCCCCAACUUCUUAGAGGGACAAGUGGCGUUCAGCCACCCGAGAUUGAGCAAUAACAGGUCUGUGAUGCCCUUAGAUGUCCGGGGCUGCACGCGCGCUACACUGACUGGCUCAGCGUGUGCCUACCCUACGCCGGCAGGCGCGGGUAACCCGUUGAACCCCAUUCGUGAUGGGGAUCGGGGAUUGCAAUUAUUCCCCAUGAACGAGGAAUUCCCAGUAAGUGCGGGUCAUAAGCUUGCGUUGAUUAAGUCCCUGCCCUUUGUACACACCGCCCGUCGCUACUACCGAUUGGAUGGUUUAGUGAGGCCCUCGGAUCGGCCCCGCCGGGGUCGGCCCACGGCCCUGGCGGAGCGCUGAGAAGACGGUCGAACUUGACUAUCUAGAGGAAGUAAAAGUCGUAACAAGGUUUCCGUAGGUGAACCUGCGGAAGGAUCAUUA

Entity 50 Entity 50 polymer

Description description	Ribosomal protein uS2
Molecular weight formula_weight	32958.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SA

MSGALDVLQMKEEDVLKFLAAGTHLGGTNLDFQMEQYIYKRKSDGIYIINLKRTWEKLLLAARAIVAIENPADVSVISSRNTGQRAVLKFAAATGATPIAGRFTPGTFTNQIQAAFREPRLLVVTDPRADHQPLTEASYVNLPTIALCNTDSPLRYVDIAIPCNNKGAHSVGLMWWMLAREVLRMRGTISREHPWEVMPDLYFYRDPEEIEKEEQAAAEKAVTKEEFQGEWTAPAPEFTATQPEVADWSEGVQVPSVPIQQFPTEDWSAQPTTEDWSAAPTAQATEWVGTTTEWS

Entity 51 Entity 51 polymer

Description description	Ribosomal protein eS1
Molecular weight formula_weight	29931.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SB

MAVGKNKRLTKGGKKGAKKKVVDPFSKRDWYDVKAPAMFNIRNIGKTLVTRTQGTKIASDGLKGRVFEVSPADLQNDEVAFRKSKLITEDVQGKNCLTNFHGMDLTRDKMCSMVKKWQTVIEAHVDVKTTDGYLLRLFCVGFTKKRNNQIRKTSYAQHQQVRQIRKKMMEIMTREVQTNDLKEVVNKLIPDSIGKDIEKACQPIYPLHDVFVRKVKMLKKPKFELGKLMELHGEGSSSGKATGDETGAKVERADGYEPPVQESV

Entity 52 Entity 52 polymer

Description description	Ribosomal protein uS5
Molecular weight formula_weight	24091.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SC

EWMPVTKLGRLVKDMKIKSLEEIYLFSLPIKESEIIDFFLGASLKDEVLKIMPVQKQTRAGQRTRFKAFVAIGDYNGHVGLGVKCSKEVATAIRGAIILAKLSIVPVRRGYWGNKIGKPHTVPCKVTGRCGSVLVRLIPAPRGTGIVSAPVPKKLLMMAGIDDCYTSARGCTATLGNFAKATFDAISKTYSYLTPDLWKETVFTKSPYQEFTDHLVKT

Entity 53 Entity 53 polymer

Description description	Ribosomal protein uS3
Molecular weight formula_weight	26729.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SD

MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPTGKIGPKKPLPDHVSIVEPKDEILPTTPISEQKGGKPEPPAMPQPVPTA

Entity 54 Entity 54 polymer

Description description	Ribosomal protein eS4
Molecular weight formula_weight	29654.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SE

MARGPKKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIIFLRNRLKYALTGDEVKKICMQRFIKIDGKVRTDITYPAGFMDVISIDKTGENFRLIYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHLVTHDARTIRYPDPLIKVNDTIQIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIGKGNKPWISLPRGKGIRLTIAEERDKRLAAKQSSG

Entity 55 Entity 55 polymer

Description description	Ribosomal protein uS7
Molecular weight formula_weight	22913.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SF

MTEWETAAPAVAETPDIKLFGKWSTDDVQINDISLQDYIAVKEKYAKYLPHSAGRYAAKRFRKAQCPIVERLTNSMMMHGRNNGKKLMTVRIVKHAFEIIHLLTGENPLQVLVNAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECLADELINAAKGSSNSYAIKKKDELERVAKSNR

Entity 56 Entity 56 polymer

Description description	Ribosomal protein eS6
Molecular weight formula_weight	28751.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SG

MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK

Entity 57 Entity 57 polymer

Description description	Ribosomal protein eS7
Molecular weight formula_weight	22168.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SH

MFSSSAKIVKPNGEKPDEFESGISQALLELEMNSDLKAQLRELNITAAKEIEVGGGRKAIIIFVPVPQLKSFQKIQVRLVRELEKKFSGKHVVFIAQRRILPKPTRKSRTKNKQKRPRSRTLTAVHDAILEDLVFPSEIVGKRIRVKLDGSRLIKVHLDKAQQNNVEHKVETFSGVYKKLTGKDVNFEFPEFQL

Entity 58 Entity 58 polymer

Description description	Ribosomal protein eS8
Molecular weight formula_weight	24263.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SI

MGISRDNWHKRRKTGGKRKPYHKKRKYELGRPAANTKIGPRRIHTVRVRGGNKKYRALRLDVGNFSWGSECCTRKTRIIDVVYNASNNELVRTKTLVKNCIVLIDSTPYRQWYESHYALPLGRKKGAKLTPEEEEILNKKRSKKIQKKYDERKKNAKISSLLEEQFQQGKLLACIASRPGQCGRADGYVLEGKELEFYLRKIKARKGK

Entity 59 Entity 59 polymer

Description description	Ribosomal protein uS4
Molecular weight formula_weight	22641.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SJ

MPVARSWVCRKTYVTPRRPFEKSRLDQELKLIGEYGLRNKREVWRVKFTLAKIRKAARELLTLDEKDPRRLFEGNALLRRLVRIGVLDEGKMKLDYILGLKIEDFLERRLQTQVFKLGLAKSIHHARVLIRQRHIRVRKQVVNIPSFIVRLDSQKHIDFSLRSPYGGGRPGRVKRKNAKKGQGGAGAGDDEEED

Entity 60 Entity 60 polymer

Description description	Ribosomal protein eS10
Molecular weight formula_weight	18967.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SK

MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARFTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ

Entity 61 Entity 61 polymer

Description description	Ribosomal protein uS17
Molecular weight formula_weight	18468.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SL

MADIQTERAYQKQPTIFQNKKRVLLGETGKEKLPRYYKNIGLGFKTPKEAIEGTYIDKKCPFTGNVSIRGRILSGVVTKMKMQRTIVIRRDYLHYIRKYNRFEKRHKNMSVHLSPCFRDVQIGDIVTVGECRPLSKTVRFNVLKVTKAAGTKKQFQKF

Entity 62 Entity 62 polymer

Description description	Ribosomal protein eS12
Molecular weight formula_weight	13776.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SM

GGVMDVNTALQEVLKTALIHDGLARGIREAAKALDKRQAHLCVQASNCDEPMYVKLVEALLAEHQINLIKVDDNKKLGEWVGLCKIDREGNPRKVVGCSCVVVKDYGKESQAKDVIEEYFKCKK

Entity 63 Entity 63 polymer

Description description	Ribosomal protein uS15
Molecular weight formula_weight	17259.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SN

MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIHRLARYYKTKRVLPPNWKYESSTASALVA

Entity 64 Entity 64 polymer

Description description	Ribosomal protein uS11
Molecular weight formula_weight	16302.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SO

MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDSTRRKGGRRGRRL

Entity 65 Entity 65 polymer

Description description	Ribosomal protein uS19
Molecular weight formula_weight	17076.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SP

MAEVEQKKKRTFRKFTYRGVDLDQLLDMSYEQLMQLYSARQRRRLNRGLRRKQHSLLKRLRKAKKEAPPMEKPEVVKTHLRDMIILPEMVGSMVGVYNGKTFNQVEIKPEMIGHYLGEFSITYKPVKHGRPGIGATHSSRFIPLK

Entity 66 Entity 66 polymer

Description description	Ribosomal protein uS9
Molecular weight formula_weight	16477.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SQ

MPSKGPLQSVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR

Entity 67 Entity 67 polymer

Description description	Ribosomal protein eS17
Molecular weight formula_weight	15552.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SR

MGRVRTKTVKKAARVIIEKYYTRLGNDFHTNKRVCEEIAIIPSKKLRNKIAGYVTHLMKRIQRGPVRGISIKLQEEERERRDNYVPEVSALDQEIIEVDPDTKEMLKLLDFGSLSNLQVTQPTVGMNFKTPRGAV

Entity 68 Entity 68 polymer

Description description	Ribosomal protein uS13
Molecular weight formula_weight	17759.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SS

MSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIPDWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK

Entity 69 Entity 69 polymer

Description description	Ribosomal protein eS19
Molecular weight formula_weight	16091.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	ST

MPGVTVKDVNQQEFVRALAAFLKKSGKLKVPEWVDTVKLAKHKELAPYDENWFYTRAASTARHLYLRGGAGVGSMTKIYGGRQRNGVMPSHFSRGSKSVARRVLQALEGLKMVEKDQDGGRKLTPQGQRDLDRIAGQVAAANKKH

Entity 70 Entity 70 polymer

Description description	Ribosomal protein uS10
Molecular weight formula_weight	13357.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SU

MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIPKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA

Entity 71 Entity 71 polymer

Description description	Ribosomal protein eS21
Molecular weight formula_weight	9124.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SV

MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQMNVAEVDKVTGRFNGQFKTYAICGAIRRMGESDDSILRLAKADGIVSKNF

Entity 72 Entity 72 polymer

Description description	Ribosomal protein uS8
Molecular weight formula_weight	14865.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SW

MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF

Entity 73 Entity 73 polymer

Description description	Ribosomal protein uS12
Molecular weight formula_weight	15854.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SX

MGKCRGLRTARKLRSHRRDHKWHDKQYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKITAFVPNDGCLNFIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKGKKERPRS

Entity 74 Entity 74 polymer

Description description	Ribosomal protein eS24
Molecular weight formula_weight	15366.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SY

MNDTVTIRTRKFMTNRLLQRKQMVIDVLHPGKATVPKTEIREKLAKMYKTTPDVIFVFGFRTHFGGGKTTGFGMIYDSLDYAKKNEPKHRLARHGLYEKKKTSRKQRKERKNRMKKVRGTAKANVGAGKKKK

Entity 75 Entity 75 polymer

Description description	Ribosomal protein es25
Molecular weight formula_weight	13776.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SZ

MPPKDDKKKKDAGKSAKKDKDPVNKSGGKAKKKKWSKGKVRDKLNNLVLFDKATYDKLCKEVPNYKLITPAVVSERLKIRGSLARAALQELLSKGLIKLVSKHRAQVIYTRNTKGGDAPAAGEDA

Entity 76 Entity 76 polymer

Description description	Ribosomal protein eS26
Molecular weight formula_weight	13047.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Sa

MTKKRRNNGRAKKGRGHVQPIRCTNCARCVPKDKAIKKFVIRNIVEAAAVRDISEASVFDAYVLPKLYVKLHYCVSCAIHSKVVRNRSREARKDRTPPPRFRPAGAAPRPPPKPM

Entity 77 Entity 77 polymer

Description description	Ribosomal protein eS27
Molecular weight formula_weight	9480.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Sb

MPLAKDLLHPSPEEEKRKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH

Entity 78 Entity 78 polymer

Description description	Ribosomal protein eS28
Molecular weight formula_weight	7855.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Sc

MDTSRVQPIKLARVTKVLGRTGSQGQCTQVRVEFMDDTSRSIIRNVKGPVREGDVLTLLESEREARRLR

Entity 79 Entity 79 polymer

Description description	Ribosomal protein uS14
Molecular weight formula_weight	6612.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Sd

MGPQGFYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIGFIKLD

Entity 80 Entity 80 polymer

Description description	Ribosomal protein eS30
Molecular weight formula_weight	14411.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Se

MQLFVRAQELHTLEVTGQETVAQIKAHVASLEGIAPEDQVVLLAGTPLEDEATLGQCGVEALTTLEVAGRMLGGKVHGSLARAGKVRGQTPKVAKQEKKKKKTGRAKRRMQYNRRFVNVVPTFGKKKGPNANS

Entity 81 Entity 81 polymer

Description description	Ribosomal protein eS31
Molecular weight formula_weight	18004.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Sf

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGAKKRKKKSYTTPKKNKHKRKKVKLAVLKYYKVDENGKISRLRRECPSDECGAGVFMASHFDRHYCGKCCLTYCFNKPEDK

Entity 82 Entity 82 polymer

Description description	Ribosomal protein RACK1
Molecular weight formula_weight	35115.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Sg

MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR

Entity 83 Entity 83 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	166

Entity 84 Entity 84 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	4

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8. Citations (1)

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	1

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	3.50 Å
Resolution测定方法 resolution_method	Single particle
颗粒数 num_particles	36667

fit	/app/data/saxs_computation/j7/3j7p/3j7p.fit
pddf	/app/data/pr_computation/j7/3j7p/3j7p.pr.out
profile	/app/data/saxs_computation/j7/3j7p/3j7p.dat