Structure of the idle mammalian ribosome-Sec61 complex

Ribosomal protein uL2

OrganismNot specified

UniProt A0A480Q387

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain A； UniProt 49–305	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain A 49–305（257 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A480Q387_PIG
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain A； PDBConstruct 1–257； UniProt 49–305

Ribosomal protein uL3

OrganismNot specified

UniProt A0A480L253

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain B； UniProt 24–417	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain B 24–417（394 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A480L253_PIG
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain B； PDBConstruct 1–394； UniProt 24–417

Ribosomal protein uL4

OrganismNot specified

UniProt A0A287AE76

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain C； UniProt 2–365	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain C 2–365（364 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A287AE76_PIG
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain C； PDBConstruct 1–367； UniProt 2–365

Ribosomal protein uL18

OrganismNot specified

UniProt A0A287ASD0

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain D； UniProt 1–297	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain D 1–297（297 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A287ASD0_PIG
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain D； PDBConstruct 1–297； UniProt 1–297

Ribosomal protein eL6

OrganismNot specified

UniProt Q2YGT9

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain E； UniProt 38–284	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain E 38–284（247 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL6_PIG
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain E； PDBConstruct 1–236； UniProt 38–284

Ribosomal protein uL30

OrganismNot specified

UniProt A0A480W0U3

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain F； UniProt 26–250	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain F 26–250（225 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A480W0U3_PIG
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain F； PDBConstruct 1–225； UniProt 26–250

Ribosomal protein eL8

OrganismNot specified

UniProt A0A4X1W808

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain G； UniProt 35–300	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain G 35–300（266 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A4X1W808_PIG
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain G； PDBConstruct 1–266； UniProt 35–300

Ribosomal protein uL6

OrganismNot specified

UniProt I3LP78

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain H； UniProt 1–192	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain H 1–192（192 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7P Structure of the 80S mammalian ribosome bound to eEF2 Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 82-meric	Chain H 1–192（192 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain H 1–192（192 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	I3LP78_PIG
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain H； PDBConstruct 1–192； UniProt 1–192

Ribosomal protein uL16

OrganismNot specified

UniProt Q29195

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain I； UniProt 2–214	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain I 2–214（213 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL10_PIG
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain I； PDBConstruct 1–213； UniProt 2–214

Ribosomal protein uL5

OrganismNot specified

UniProt Q29205

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain J； UniProt 1–178	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain J 1–178（178 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7P Structure of the 80S mammalian ribosome bound to eEF2 Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 82-meric	Chain J 1–178（178 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain J 1–178（178 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL11_PIG
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain J； PDBConstruct 1–178； UniProt 1–178

Ribosomal protein eL13

OrganismNot specified

UniProt A0A4X1TBV3

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain L； UniProt 218–428	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain L 218–428（211 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A4X1TBV3_PIG
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain L； PDBConstruct 1–211； UniProt 218–428

Ribosomal protein eL14

OrganismNot specified

UniProt A1XQU3

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain M； UniProt 1–213	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain M 1–213（213 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7P Structure of the 80S mammalian ribosome bound to eEF2 Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 82-meric	Chain M 1–213（213 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain M 1–213（213 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL14_PIG
Isoform	—
PDB entities	15
Chains and sequence ranges	Author chain M； PDBConstruct 1–213； UniProt 1–213

Ribosomal protein eL15

OrganismNot specified

UniProt A0A286ZL65

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain N； UniProt 1–204	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain N 1–204（204 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A286ZL65_PIG
Isoform	—
PDB entities	16
Chains and sequence ranges	Author chain N； PDBConstruct 1–204； UniProt 1–204

Ribosomal protein uL13

OrganismNot specified

UniProt A0A481CAM4

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain O； UniProt 63–261	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence

UniProt name	A0A481CAM4_PIG
Isoform	—
PDB entities	17
Chains and sequence ranges	Author chain O； PDBConstruct 1–201； UniProt 63–261

Ribosomal protein uL22

OrganismNot specified

UniProt A0A287B386

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain P； UniProt 2–154	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain P 2–154（153 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A287B386_PIG
Isoform	—
PDB entities	18
Chains and sequence ranges	Author chain P； PDBConstruct 1–153； UniProt 2–154

Ribosomal protein eL18

OrganismNot specified

UniProt A0A4X1W0N5

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain Q； UniProt 1–188	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain Q 1–188（188 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A4X1W0N5_PIG
Isoform	—
PDB entities	19
Chains and sequence ranges	Author chain Q； PDBConstruct 1–188； UniProt 1–188

Ribosomal protein eL19

OrganismNot specified

UniProt A0A480VXS3

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain R； UniProt 42–236	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain R 42–236（195 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A480VXS3_PIG
Isoform	—
PDB entities	20
Chains and sequence ranges	Author chain R； PDBConstruct 2–196； UniProt 42–236

Ribosomal protein eL20

OrganismNot specified

UniProt A0A287APR1

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain S； UniProt 1–224	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain S 1–224（224 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å
6MTD Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) Deposited 2018-10-19	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric	Chain S 49–224（176 aa）	Not recorded	MG MAGNESIUM ION × 297 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
6MTE Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) Deposited 2018-10-19	Different construct Different oligomeric state Different ligand/ion Different experimental conditions	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric	Chain S 49–224（176 aa）	Not recorded	MG MAGNESIUM ION × 298 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain S3 49–224（176 aa）	Not recorded	MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.	Resolution 3.80 Å
7NWH Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S. Deposited 2021-03-16	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric	Chain S 49–224（176 aa）	Not recorded	ZN ZINC ION × 8 MG MAGNESIUM ION × 281 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 BLS BLASTICIDIN S × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.	Resolution 4.10 Å
8RJB Structure of the rabbit 80S ribosome stalled on a 2-TMD rhodopsin intermediate in complex with Sec61-RAMP4 Deposited 2023-12-20	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 51-meric	Chain S 49–224（176 aa）	Not recorded	MG MAGNESIUM ION × 218 ZN ZINC ION × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.69 Å
8RJC Structure of the rabbit 80S ribosome stalled on a 2-TMD rhodopsin intermediate in complex with Sec61-TRAP, open conformation 1 Deposited 2023-12-20	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 56-meric	Chain S 49–224（176 aa）	Not recorded	MG MAGNESIUM ION × 218 ZN ZINC ION × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
8RJD Structure of the rabbit 80S ribosome stalled on a 2-TMD rhodopsin intermediate in complex with Sec61-TRAP, open conformation 2 Deposited 2023-12-20	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 56-meric	Chain S 49–224（176 aa）	Not recorded	MG MAGNESIUM ION × 218 ZN ZINC ION × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.79 Å

8 other PDB entries and 8 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A287APR1_PIG
Isoform	—
PDB entities	21
Chains and sequence ranges	Author chain S； PDBConstruct 1–224； UniProt 1–224

Ribosomal protein eL21

OrganismNot specified

UniProt P49666

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain T； UniProt 1–160	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain T 1–160（160 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL21_PIG
Isoform	—
PDB entities	22
Chains and sequence ranges	Author chain T； PDBConstruct 1–160； UniProt 1–160

Ribosomal protein eL22

OrganismNot specified

UniProt P67985

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain U； UniProt 1–128	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain U 1–128（128 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7P Structure of the 80S mammalian ribosome bound to eEF2 Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 82-meric	Chain U 1–128（128 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain U 1–128（128 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

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UniProt name	RL22_PIG
Isoform	—
PDB entities	23
Chains and sequence ranges	Author chain U； PDBConstruct 1–128； UniProt 1–128

Ribosomal protein uL14

OrganismNot specified

UniProt A0A480VZZ1

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain V； UniProt 1–140	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain V 1–140（140 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A480VZZ1_PIG
Isoform	—
PDB entities	24
Chains and sequence ranges	Author chain V； PDBConstruct 1–140； UniProt 1–140

Ribosomal protein eL24

OrganismNot specified

UniProt A0A4X1SH42

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain W； UniProt 1–157	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain W 1–157（157 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A4X1SH42_PIG
Isoform	—
PDB entities	25
Chains and sequence ranges	Author chain W； PDBConstruct 1–157； UniProt 1–157

Ribosomal protein uL23

OrganismNot specified

UniProt A0A480KZ98

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain X； UniProt 56–211	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain X 56–211（156 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A480KZ98_PIG
Isoform	—
PDB entities	26
Chains and sequence ranges	Author chain X； PDBConstruct 1–156； UniProt 56–211

Ribosomal protein uL24

OrganismNot specified

UniProt A0A4X1V977

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain Y； UniProt 1–145	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain Y 1–145（145 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A4X1V977_PIG
Isoform	—
PDB entities	27
Chains and sequence ranges	Author chain Y； PDBConstruct 1–145； UniProt 1–145

Ribosomal protein eL27

OrganismNot specified

UniProt A1XQU5

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain Z； UniProt 1–136	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain Z 1–136（136 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7P Structure of the 80S mammalian ribosome bound to eEF2 Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 82-meric	Chain Z 1–136（136 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain Z 1–136（136 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL27_PIG
Isoform	—
PDB entities	28
Chains and sequence ranges	Author chain Z； PDBConstruct 1–136； UniProt 1–136

Ribosomal protein uL15

OrganismNot specified

UniProt A0A4X1SN08

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain a； UniProt 116–263	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain a 116–263（148 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A4X1SN08_PIG
Isoform	—
PDB entities	29
Chains and sequence ranges	Author chain a； PDBConstruct 1–148； UniProt 116–263

Ribosomal protein eL29

OrganismNot specified

UniProt Q95281

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain b； UniProt 1–160	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain b 1–160（160 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7P Structure of the 80S mammalian ribosome bound to eEF2 Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 82-meric	Chain b 1–160（160 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain b 1–160（160 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL29_PIG
Isoform	—
PDB entities	30
Chains and sequence ranges	Author chain b； PDBConstruct 1–160； UniProt 1–160

Ribosomal protein eL30

OrganismNot specified

UniProt A0A4X1TXJ8

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain c； UniProt 1–115	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain c 1–115（115 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A4X1TXJ8_PIG
Isoform	—
PDB entities	31
Chains and sequence ranges	Author chain c； PDBConstruct 1–115； UniProt 1–115

Ribosomal protein eL31

OrganismNot specified

UniProt P62901

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain d； UniProt 1–125	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain d 1–125（125 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7P Structure of the 80S mammalian ribosome bound to eEF2 Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 82-meric	Chain d 1–125（125 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain d 1–125（125 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL31_PIG
Isoform	—
PDB entities	32
Chains and sequence ranges	Author chain d； PDBConstruct 1–125； UniProt 1–125

Ribosomal protein eL32

OrganismNot specified

UniProt Q6QAT0

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain e； UniProt 1–135	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain e 1–135（135 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7P Structure of the 80S mammalian ribosome bound to eEF2 Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 82-meric	Chain e 1–135（135 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain e 1–135（135 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL32_PIG
Isoform	—
PDB entities	33
Chains and sequence ranges	Author chain e； PDBConstruct 1–135； UniProt 1–135

Ribosomal protein eL33

OrganismNot specified

UniProt A0A480KUB2

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain f； UniProt 25–134	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain f 25–134（110 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A480KUB2_PIG
Isoform	—
PDB entities	34
Chains and sequence ranges	Author chain f； PDBConstruct 1–110； UniProt 25–134

Ribosomal protein eL34

OrganismNot specified

UniProt Q29223

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain g； UniProt 1–117	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain g 1–117（117 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL34_PIG
Isoform	—
PDB entities	35
Chains and sequence ranges	Author chain g； PDBConstruct 1–117； UniProt 1–117

Ribosomal protein uL29

OrganismNot specified

UniProt Q29361

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain h； UniProt 1–123	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain h 1–123（123 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7P Structure of the 80S mammalian ribosome bound to eEF2 Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 82-meric	Chain h 1–123（123 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain h 1–123（123 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL35_PIG
Isoform	—
PDB entities	36
Chains and sequence ranges	Author chain h； PDBConstruct 1–123； UniProt 1–123

Ribosomal protein eL36

OrganismNot specified

UniProt A0A480QBP0

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain i； UniProt 103–207	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain i 103–207（105 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A480QBP0_PIG
Isoform	—
PDB entities	37
Chains and sequence ranges	Author chain i； PDBConstruct 1–105； UniProt 103–207

Ribosomal protein eL37

OrganismNot specified

UniProt A0A480UVT3

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain j； UniProt 2–87	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain j 2–87（86 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A480UVT3_PIG
Isoform	—
PDB entities	38
Chains and sequence ranges	Author chain j； PDBConstruct 1–86； UniProt 2–87

Ribosomal protein eL38

OrganismNot specified

UniProt F2Z568

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain k； UniProt 1–70	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain k 1–70（70 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7P Structure of the 80S mammalian ribosome bound to eEF2 Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 82-meric	Chain k 1–70（70 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain k 1–70（70 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	F2Z568_PIG
Isoform	—
PDB entities	39
Chains and sequence ranges	Author chain k； PDBConstruct 1–70； UniProt 1–70

Ribosomal protein eL39

OrganismNot specified

UniProt A0A4X1VIM4

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain l； UniProt 1–51	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain l 1–51（51 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A4X1VIM4_PIG
Isoform	—
PDB entities	40
Chains and sequence ranges	Author chain l； PDBConstruct 1–51； UniProt 1–51

Ribosomal protein eL40

OrganismNot specified

UniProt P63053

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain m； UniProt 1–128	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7O Structure of the mammalian 60S ribosomal subunit Deposited 2014-08-01	Different oligomeric state	Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric	Chain m 1–128（128 aa）	Not recorded	MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å
3J7P Structure of the 80S mammalian ribosome bound to eEF2 Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 82-meric	Chain m 1–128（128 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.50 Å
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain m 1–128（128 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL40_PIG
Isoform	—
PDB entities	41
Chains and sequence ranges	Author chain m； PDBConstruct 1–128； UniProt 1–128

Ribosomal protein eL42

OrganismNot specified

UniProt A0A480LCM6

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain o； UniProt 37–142	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain o 37–142（106 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A480LCM6_PIG
Isoform	—
PDB entities	43
Chains and sequence ranges	Author chain o； PDBConstruct 1–106； UniProt 37–142

Ribosomal protein eL43

OrganismNot specified

UniProt A0A480WPS5

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain p； UniProt 20–110	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain p 20–110（91 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A480WPS5_PIG
Isoform	—
PDB entities	44
Chains and sequence ranges	Author chain p； PDBConstruct 1–91； UniProt 20–110

Ribosomal protein eL28

OrganismNot specified

UniProt A0A480YX24

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain r； UniProt 32–156	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain r 32–156（125 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A480YX24_PIG
Isoform	—
PDB entities	45
Chains and sequence ranges	Author chain r； PDBConstruct 1–125； UniProt 32–156

Sec61 alpha subunit

OrganismNot specified

UniProt A0A480EHF8

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain 1； UniProt 113–588	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 gamma subunit × 1 （A0A480WFL1） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence

UniProt name	A0A480EHF8_PIG
Isoform	—
PDB entities	46
Chains and sequence ranges	Author chain 1； PDBConstruct 1–476； UniProt 113–588

Sec61 gamma subunit

OrganismNot specified

UniProt A0A480WFL1

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 45 RNA 3 PDB declaration: 48-meric（48） Consistent with all polymer counts	Chain 2； UniProt 1–68	Not recorded	28S ribosomal RNA × 1 5S ribosomal RNA × 1 5.8S ribosomal RNA × 1 Ribosomal protein uL2 × 1 （A0A480Q387） Ribosomal protein uL3 × 1 （A0A480L253） Ribosomal protein uL4 × 1 （A0A287AE76） Ribosomal protein uL18 × 1 （A0A287ASD0） Ribosomal protein eL6 × 1 （Q2YGT9） Ribosomal protein uL30 × 1 （A0A480W0U3） Ribosomal protein eL8 × 1 （A0A4X1W808） Ribosomal protein uL6 × 1 （I3LP78） Ribosomal protein uL16 × 1 （Q29195） Ribosomal protein uL5 × 1 （Q29205） Ribosomal protein eL13 × 1 （A0A4X1TBV3） Ribosomal protein eL14 × 1 （A1XQU3） Ribosomal protein eL15 × 1 （A0A286ZL65） Ribosomal protein uL13 × 1 （A0A481CAM4） Ribosomal protein uL22 × 1 （A0A287B386） Ribosomal protein eL18 × 1 （A0A4X1W0N5） Ribosomal protein eL19 × 1 （A0A480VXS3） Ribosomal protein eL20 × 1 （A0A287APR1） Ribosomal protein eL21 × 1 （P49666） Ribosomal protein eL22 × 1 （P67985） Ribosomal protein uL14 × 1 （A0A480VZZ1） Ribosomal protein eL24 × 1 （A0A4X1SH42） Ribosomal protein uL23 × 1 （A0A480KZ98） Ribosomal protein uL24 × 1 （A0A4X1V977） Ribosomal protein eL27 × 1 （A1XQU5） Ribosomal protein uL15 × 1 （A0A4X1SN08） Ribosomal protein eL29 × 1 （Q95281） Ribosomal protein eL30 × 1 （A0A4X1TXJ8） Ribosomal protein eL31 × 1 （P62901） Ribosomal protein eL32 × 1 （Q6QAT0） Ribosomal protein eL33 × 1 （A0A480KUB2） Ribosomal protein eL34 × 1 （Q29223） Ribosomal protein uL29 × 1 （Q29361） Ribosomal protein eL36 × 1 （A0A480QBP0） Ribosomal protein eL37 × 1 （A0A480UVT3） Ribosomal protein eL38 × 1 （F2Z568） Ribosomal protein eL39 × 1 （A0A4X1VIM4） Ribosomal protein eL40 × 1 （P63053） Ribosomal protein eL41 × 1 Ribosomal protein eL42 × 1 （A0A480LCM6） Ribosomal protein eL43 × 1 （A0A480WPS5） Ribosomal protein eL28 × 1 （A0A480YX24） Sec61 alpha subunit × 1 （A0A480EHF8） Sec61 beta subunit × 1 MG MAGNESIUM ION × 130 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer：50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions：120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 3J7Q	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3J7R Structure of the translating mammalian ribosome-Sec61 complex Deposited 2014-08-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric	Chain 2 1–68（68 aa）	Not recorded	MG MAGNESIUM ION × 166 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin；pH 7.5；50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin cryo-EM vitrification conditions 120 K；Cryogen ETHANE；3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A480WFL1_PIG
Isoform	—
PDB entities	47
Chains and sequence ranges	Author chain 2； PDBConstruct 1–68； UniProt 1–68

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.