← Back to search
3syu

Re-refined coordinates for pdb entry 1det - ribonuclease T1 carboxymethylated at GLU 58 in complex with 2'GMP

Method: X-RAY DIFFRACTION
▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

Guanyl-specific ribonuclease T1

OrganismNot specified

UniProt P00651

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 27–130 Non-standard monomer：Yes (specific site not provided by mmCIF) NA SODIUM ION × 2 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 UNX UNKNOWN LIGAND × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 1.95 Å R-free 0.165

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 3SYU Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1B2M THREE-DIMENSIONAL STRUCTURE OF RIBONULCEASE T1 COMPLEXED WITH AN ISOSTERIC PHOSPHONATE ANALOGUE OF GPU: ALTERNATE SUBSTRATE BINDING MODES AND CATALYSIS. Deposited 1998-11-27 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric
Chain A 27–130（104 aa）
Mutation：GLN 25 VARIANT No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.1；pH 4.10
Resolution 2.00 Å R-free 0.254
1B2M THREE-DIMENSIONAL STRUCTURE OF RIBONULCEASE T1 COMPLEXED WITH AN ISOSTERIC PHOSPHONATE ANALOGUE OF GPU: ALTERNATE SUBSTRATE BINDING MODES AND CATALYSIS. Deposited 1998-11-27 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Monomer；Protein × 1 PDB declaration: trimeric
Chain B 27–130（104 aa）
Mutation：GLN 25 VARIANT No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.1；pH 4.10
Resolution 2.00 Å R-free 0.254
1BIR RIBONUCLEASE T1, PHE 100 TO ALA MUTANT COMPLEXED WITH 2' GMP Deposited 1996-01-04 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：F100A CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å R-free 0.240
1BIR RIBONUCLEASE T1, PHE 100 TO ALA MUTANT COMPLEXED WITH 2' GMP Deposited 1996-01-04 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 27–130（104 aa）
Mutation：F100A CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å R-free 0.240
1BU4 RIBONUCLEASE 1 COMPLEX WITH 2'GMP Deposited 1998-09-11 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Not recorded CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；pH 4.5
Resolution 1.90 Å R-free 0.230
1BVI RIBONUCLEASE T1 (WILDTYPE) COMPLEXED WITH 2'GMP Deposited 1998-09-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 27–130（104 aa）
Chain B 27–130（104 aa）
Chain C 27–130（104 aa）
Chain D 27–130（104 aa）
Not recorded 2GP GUANOSINE-2'-MONOPHOSPHATE × 5 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；25 MM NAAC PH 4.2 6.25 MM CACL2 47.5 % MPD
Resolution 1.90 Å R-free 0.219
1CH0 RNASE T1 VARIANT WITH ALTERED GUANINE BINDING SEGMENT Deposited 1999-03-30 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：Q25K, K41E, Y42F, N43R, Y45W, E46Q CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.7；HANGING DROP CONTAINING 2.5 MG/ML RNASE T1, 2.5 MG/ML 2GMP, 15% PEG MONOMETHYLETHER 5000, 50 MM TRIS/HCL (PH7.7), 50 MM MGCL2, 50 MM CACL2 AGAINST 30 % PEG MONOMETHYLETHER 5000, 100 MM TRIS/HCL (PH7.7), 100 MM MGCL2, 100 MM CACL2
Resolution 2.30 Å R-free 0.241
1CH0 RNASE T1 VARIANT WITH ALTERED GUANINE BINDING SEGMENT Deposited 1999-03-30 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 27–130（104 aa）
Mutation：Q25K, K41E, Y42F, N43R, Y45W, E46Q CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.7；HANGING DROP CONTAINING 2.5 MG/ML RNASE T1, 2.5 MG/ML 2GMP, 15% PEG MONOMETHYLETHER 5000, 50 MM TRIS/HCL (PH7.7), 50 MM MGCL2, 50 MM CACL2 AGAINST 30 % PEG MONOMETHYLETHER 5000, 100 MM TRIS/HCL (PH7.7), 100 MM MGCL2, 100 MM CACL2
Resolution 2.30 Å R-free 0.241
1CH0 RNASE T1 VARIANT WITH ALTERED GUANINE BINDING SEGMENT Deposited 1999-03-30 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 27–130（104 aa）
Mutation：Q25K, K41E, Y42F, N43R, Y45W, E46Q CA CALCIUM ION × 2 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.7；HANGING DROP CONTAINING 2.5 MG/ML RNASE T1, 2.5 MG/ML 2GMP, 15% PEG MONOMETHYLETHER 5000, 50 MM TRIS/HCL (PH7.7), 50 MM MGCL2, 50 MM CACL2 AGAINST 30 % PEG MONOMETHYLETHER 5000, 100 MM TRIS/HCL (PH7.7), 100 MM MGCL2, 100 MM CACL2
Resolution 2.30 Å R-free 0.241
1DET RIBONUCLEASE T1 CARBOXYMETHYLATED AT GLU 58 IN COMPLEX WITH 2'GMP Deposited 1996-02-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) NA SODIUM ION × 2 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；pH 4.5
Resolution 1.80 Å
1FYS Ribonuclease T1 V16C mutant Deposited 2000-10-03 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：V16C CA CALCIUM ION × 2 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；MPD, Na-acetate, calcium chloride, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.00 Å R-free 0.208
1FZU RNAse T1 V78A mutant Deposited 2000-10-04 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：V78A CA CALCIUM ION × 2 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；MPD, calcium chloride, sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.80 Å R-free 0.198
1G02 Ribonuclease T1 V16S mutant Deposited 2000-10-05 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：V16S CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；MPD, calcium chloride, sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.86 Å R-free 0.210
1GSP RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS, 1 DAY Deposited 1997-11-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–125（104 aa）
Not recorded CA CALCIUM ION × 1 SGP GUANOSINE-2',3'-CYCLOPHOSPHOROTHIOATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；20 MM NAAC PH 4.2 2 MM CACL2 55 % MPD
Resolution 2.20 Å R-free 0.206
1HYF RIBONUCLEASE T1 V16A MUTANT IN COMPLEX WITH SR2+ Deposited 2001-01-19 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：V16A SR STRONTIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；MPD, calcium chloride, acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.70 Å R-free 0.203
1HZ1 RIBONUCLEASE T1 V16A MUTANT IN COMPLEX WITH MG2+ Deposited 2001-01-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–125（104 aa）
Mutation：V16A MG MAGNESIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；MPD, acetate, calcium chloride, magnesiusm chrloride (soak), pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.80 Å R-free 0.214
1I0V Ribonuclease T1 in complex with 2'GMP (form I crystal) Deposited 2001-01-30 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：Q25K CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；MPD, acetaate, calcium chloride, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.23 Å R-free 0.192
1I0X RIBONUCLEASE T1 IN COMPLEX WITH 2'GMP (FORM II CRYSTAL) Deposited 2001-01-30 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：Q25K 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；MPD, acetate, calcium chloride, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.65 Å R-free 0.216
1I0X RIBONUCLEASE T1 IN COMPLEX WITH 2'GMP (FORM II CRYSTAL) Deposited 2001-01-30 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 27–130（104 aa）
Mutation：Q25K 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；MPD, acetate, calcium chloride, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.65 Å R-free 0.216
1I0X RIBONUCLEASE T1 IN COMPLEX WITH 2'GMP (FORM II CRYSTAL) Deposited 2001-01-30 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 27–130（104 aa）
Mutation：Q25K 2GP GUANOSINE-2'-MONOPHOSPHATE × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；MPD, acetate, calcium chloride, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.65 Å R-free 0.216
1I0X RIBONUCLEASE T1 IN COMPLEX WITH 2'GMP (FORM II CRYSTAL) Deposited 2001-01-30 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 27–130（104 aa）
Mutation：Q25K 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；MPD, acetate, calcium chloride, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.65 Å R-free 0.216
1I0X RIBONUCLEASE T1 IN COMPLEX WITH 2'GMP (FORM II CRYSTAL) Deposited 2001-01-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 5 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 27–130（104 aa）
Chain D 27–130（104 aa）
Mutation：Q25K Mutation：Q25K 2GP GUANOSINE-2'-MONOPHOSPHATE × 3 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；MPD, acetate, calcium chloride, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.65 Å R-free 0.216
1I2E Ribonuclease T1 V16A mutant, form I Deposited 2001-02-09 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：V16A, Q25K CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；MPD, calcium chloride, sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.80 Å R-free 0.209
1I2F Ribonuclease T1 V16A mutant, form II Deposited 2001-02-09 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：V16A, Q25K CA CALCIUM ION × 2 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；MPD, calcium chloride, sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.95 Å R-free 0.205
1I2G Ribonuclease T1 V16T mutant Deposited 2001-02-09 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：V16T, Q25K CA CALCIUM ION × 2 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；MPD, calcium chloride, sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.85 Å R-free 0.197
1I3F Ribonuclease T1 V89S mutant Deposited 2001-02-15 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：V89S, Q25K CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；MPD, sodium acetate, calcium chloride, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.35 Å R-free 0.283
1I3I Ribonuclease T1 V78T mutant Deposited 2001-02-15 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：V78T, Q25K CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；MPD, calcium chloride, sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.76 Å R-free 0.202
1LOV X-ray structure of the E58A mutant of Ribonuclease T1 complexed with 3'-guanosine monophosphate Deposited 2002-05-07 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：E58A CA CALCIUM ION × 1 3GP GUANOSINE-3'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；MPD,CaCl2,sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.55 Å R-free 0.199
1LOW X-ray structure of the H40A mutant of Ribonuclease T1 complexed with 3'-guanosine monophosphate Deposited 2002-05-07 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：H40A CA CALCIUM ION × 1 3GP GUANOSINE-3'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；MPD,CaCl2,sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.90 Å R-free 0.229
1LOY X-ray structure of the H40A/E58A mutant of Ribonuclease T1 complexed with 3'-guanosine monophosphate Deposited 2002-05-07 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：H40A/E58A CA CALCIUM ION × 1 3GP GUANOSINE-3'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；MPD,CaCl2,sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.55 Å R-free 0.218
1LRA CRYSTALLOGRAPHIC STUDY OF GLU 58 ALA RNASE T1(ASTERISK)2'-GUANOSINE MONOPHOSPHATE AT 1.9 ANGSTROMS RESOLUTION Deposited 1993-10-01 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Not recorded NA SODIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å
1Q9E RNase T1 variant with adenine specificity Deposited 2003-08-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–104（104 aa）
Mutation：K41E, Y42F, N43R, Y45W, E46N TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 9；279 K；PEG4000, magnesium chloride, Tris, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 279K, pH 9.00
Resolution 1.70 Å R-free 0.244
1Q9E RNase T1 variant with adenine specificity Deposited 2003-08-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–104（104 aa）
Mutation：K41E, Y42F, N43R, Y45W, E46N No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 9；279 K；PEG4000, magnesium chloride, Tris, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 279K, pH 9.00
Resolution 1.70 Å R-free 0.244
1Q9E RNase T1 variant with adenine specificity Deposited 2003-08-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 1–104（104 aa）
Mutation：K41E, Y42F, N43R, Y45W, E46N TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 9；279 K；PEG4000, magnesium chloride, Tris, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 279K, pH 9.00
Resolution 1.70 Å R-free 0.244
1RGA CRYSTAL STRUCTURE OF RNASE T1 WITH 3'-GMP AND GUANOSINE: A PRODUCT COMPLEX Deposited 1993-06-07 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–125（104 aa）
Not recorded CA CALCIUM ION × 1 3GP GUANOSINE-3'-MONOPHOSPHATE × 1 GMP GUANOSINE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.70 Å
1RGC THE COMPLEX BETWEEN RIBONUCLEASE T1 AND 3'-GUANYLIC ACID SUGGESTS GEOMETRY OF ENZYMATIC REACTION PATH. AN X-RAY STUDY Deposited 1993-05-12 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–125（104 aa）
Not recorded CA CALCIUM ION × 1 3GP GUANOSINE-3'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
1RGC THE COMPLEX BETWEEN RIBONUCLEASE T1 AND 3'-GUANYLIC ACID SUGGESTS GEOMETRY OF ENZYMATIC REACTION PATH. AN X-RAY STUDY Deposited 1993-05-12 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 22–125（104 aa）
Not recorded CA CALCIUM ION × 1 3GP GUANOSINE-3'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
1RGK RNASE T1 MUTANT GLU46GLN BINDS THE INHIBITORS 2'GMP AND 2'AMP AT THE 3' SUBSITE Deposited 1992-02-19 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Not recorded CA CALCIUM ION × 1 2AM ADENOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.87 Å
1RGL RNASE T1 MUTANT GLU46GLN BINDS THE INHIBITORS 2'GMP AND 2'AMP AT THE 3' SUBSITE Deposited 1992-02-19 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Not recorded CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
1RHL RIBONUCLEASE T1 COMPLEXED WITH 2'GMP/G23A MUTANT Deposited 1998-10-09 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：G23A CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；50 MM NAAC PH 4.2 2 MM CACL2 50 % MPD 2MM 2`GMP
Resolution 1.95 Å R-free 0.208
1RLS CRYSTAL STRUCTURE OF RNASE T1 COMPLEXED WITH THE PRODUCT NUCLEOTIDE 3'-GMP. STRUCTURAL EVIDENCE FOR DIRECT INTERACTION OF HISTIDINE 40 AND GLUTAMIC ACID 58 WITH THE 2'-HYDROXYL GROUP OF RIBOSE Deposited 1994-03-29 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Not recorded CA CALCIUM ION × 1 3GP GUANOSINE-3'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å
1RN1 THREE-DIMENSIONAL STRUCTURE OF GLN 25-RIBONUCLEASE T1 AT 1.84 ANGSTROMS RESOLUTION: STRUCTURAL VARIATIONS AT THE BASE RECOGNITION AND CATALYTIC SITES Deposited 1991-11-22 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 27–130（104 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.84 Å
1RN1 THREE-DIMENSIONAL STRUCTURE OF GLN 25-RIBONUCLEASE T1 AT 1.84 ANGSTROMS RESOLUTION: STRUCTURAL VARIATIONS AT THE BASE RECOGNITION AND CATALYTIC SITES Deposited 1991-11-22 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.84 Å
1RN1 THREE-DIMENSIONAL STRUCTURE OF GLN 25-RIBONUCLEASE T1 AT 1.84 ANGSTROMS RESOLUTION: STRUCTURAL VARIATIONS AT THE BASE RECOGNITION AND CATALYTIC SITES Deposited 1991-11-22 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 27–130（104 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.84 Å
1RN4 HIS92ALA MUTATION IN RIBONUCLEASE T1 INDUCES SEGMENTAL FLEXIBILITY. AN X-RAY STUDY Deposited 1991-11-07 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Not recorded PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å
1RNT RESTRAINED LEAST-SQUARES REFINEMENT OF THE CRYSTAL STRUCTURE OF THE RIBONUCLEASE T1(ASTERISK)2(PRIME)-GUANYLIC ACID COMPLEX AT 1.9 ANGSTROMS RESOLUTION Deposited 1987-07-10 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–125（104 aa）
Not recorded 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å
1TRP X-RAY CRYSTALLOGRAPHIC AND CALORIMERIC STUDIES OF THE EFFECTS OF THE MUTATION TRP 59 TYR IN RIBONUCLEASE T1 Deposited 1993-07-06 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Not recorded CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.40 Å
1TRP X-RAY CRYSTALLOGRAPHIC AND CALORIMERIC STUDIES OF THE EFFECTS OF THE MUTATION TRP 59 TYR IN RIBONUCLEASE T1 Deposited 1993-07-06 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 27–130（104 aa）
Not recorded CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.40 Å
1TRQ X-RAY CRYSTALLOGRAPHIC AND CALORIMERIC STUDIES OF THE EFFECTS OF THE MUTATION TRP 59 TYR IN RIBONUCLEASE T1 Deposited 1993-07-06 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Not recorded CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.30 Å
1TRQ X-RAY CRYSTALLOGRAPHIC AND CALORIMERIC STUDIES OF THE EFFECTS OF THE MUTATION TRP 59 TYR IN RIBONUCLEASE T1 Deposited 1993-07-06 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 27–130（104 aa）
Not recorded CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.30 Å
1TTO Crystal structure of the Rnase T1 variant R2 Deposited 2004-06-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–104（104 aa）
Mutation：k41e, y42f, n43r, y45w, e46n, w59y TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；278 K；PEG4000, tris-Cl, calcium chloride, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 278.0K
Resolution 2.10 Å R-free 0.238
1TTO Crystal structure of the Rnase T1 variant R2 Deposited 2004-06-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–104（104 aa）
Mutation：k41e, y42f, n43r, y45w, e46n, w59y TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；278 K；PEG4000, tris-Cl, calcium chloride, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 278.0K
Resolution 2.10 Å R-free 0.238
1TTO Crystal structure of the Rnase T1 variant R2 Deposited 2004-06-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 1–104（104 aa）
Mutation：k41e, y42f, n43r, y45w, e46n, w59y TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；278 K；PEG4000, tris-Cl, calcium chloride, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 278.0K
Resolution 2.10 Å R-free 0.238
1YGW NMR STRUCTURE OF RIBONUCLEASE T1, 34 STRUCTURES Deposited 1996-09-28 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–125（104 aa）
Mutation：ISOENZYME WITH LYSINE AT POSITION 25 No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 5.5；313 K
Resolution not provided
2AAD THE ROLE OF HISTIDINE-40 IN RIBONUCLEASE T1 CATALYSIS: THREE-DIMENSIONAL STRUCTURES OF THE PARTIALLY ACTIVE HIS40LYS MUTANT Deposited 1992-09-15 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Not recorded CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
2AAD THE ROLE OF HISTIDINE-40 IN RIBONUCLEASE T1 CATALYSIS: THREE-DIMENSIONAL STRUCTURES OF THE PARTIALLY ACTIVE HIS40LYS MUTANT Deposited 1992-09-15 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 27–130（104 aa）
Not recorded CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
2AAE THE ROLE OF HISTIDINE-40 IN RIBONUCLEASE T1 CATALYSIS: THREE-DIMENSIONAL STRUCTURES OF THE PARTIALLY ACTIVE HIS40LYS MUTANT Deposited 1992-09-15 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Not recorded CA CALCIUM ION × 1 PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å
2BIR ADDITIVITY OF SUBSTRATE BINDING IN RIBONUCLEASE T1 (Y42A MUTANT) Deposited 1996-12-03 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：YES CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；pH 4.20
Resolution 2.30 Å R-free 0.257
2BU4 RIBONUCLEASE T1 COMPLEX WITH 2'GMP Deposited 1998-09-14 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Not recorded CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；20 MM NA-ACETATE PH 4.2 2 MM CACL2 55 % MPD
Resolution 1.95 Å R-free 0.249
2GSP RIBONUCLEASE T1/2',3'-CGPS AND 3'-GMP, 2 DAYS Deposited 1997-12-02 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–125（104 aa）
Not recorded CA CALCIUM ION × 1 SGP GUANOSINE-2',3'-CYCLOPHOSPHOROTHIOATE × 1 3GP GUANOSINE-3'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；20 MM NAAC PH 4.2 2 MM CACL2 55 % MPD
Resolution 1.80 Å R-free 0.197
2HOH RIBONUCLEASE T1 (N9A MUTANT) COMPLEXED WITH 2'GMP Deposited 1998-09-14 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：N9A 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；25 MM NAAC PH 4.2 6.25 MM CACL2 45.0 % MPD
Resolution 1.90 Å R-free 0.209
2HOH RIBONUCLEASE T1 (N9A MUTANT) COMPLEXED WITH 2'GMP Deposited 1998-09-14 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 27–130（104 aa）
Mutation：N9A 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 NA SODIUM ION × 1 PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；25 MM NAAC PH 4.2 6.25 MM CACL2 45.0 % MPD
Resolution 1.90 Å R-free 0.209
2HOH RIBONUCLEASE T1 (N9A MUTANT) COMPLEXED WITH 2'GMP Deposited 1998-09-14 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 27–130（104 aa）
Mutation：N9A 2GP GUANOSINE-2'-MONOPHOSPHATE × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；25 MM NAAC PH 4.2 6.25 MM CACL2 45.0 % MPD
Resolution 1.90 Å R-free 0.209
2HOH RIBONUCLEASE T1 (N9A MUTANT) COMPLEXED WITH 2'GMP Deposited 1998-09-14 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 27–130（104 aa）
Mutation：N9A 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；25 MM NAAC PH 4.2 6.25 MM CACL2 45.0 % MPD
Resolution 1.90 Å R-free 0.209
2RNT THREE-DIMENSIONAL STRUCTURE OF RIBONUCLEASE T1 COMPLEXED WITH GUANYLYL-2(PRIME),5(PRIME)-GUANOSINE AT 1.8 ANGSTROMS RESOLUTION Deposited 1988-07-06 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–125（104 aa）
Not recorded CA CALCIUM ION × 1 GPG GUANYLYL-2',5'-PHOSPHOGUANOSINE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å
3BIR DISECTING HISTIDINE INTERACTIONS IN RIBONUCLEASE T1 BY ASN AND GLN SUBSTITUTIONS Deposited 1997-06-27 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：H92N CA CALCIUM ION × 2 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；VAPOR DIFFUSION, NAOAC PH 4.2, 20 MG/ML PROTEIN, 45 % MPD 2.5 MM CACL2, 20 MM GPS
Resolution 1.80 Å R-free 0.237
3BU4 RIBONUCLEASE T1 COMPLEX WITH 2'GMP Deposited 1998-09-14 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Not recorded CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；20 MM NA-ACETATE PH 4.2 2 MM CACL2 55 % MPD
Resolution 1.77 Å R-free 0.249
3GSP RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS + 3'-GMP, 4 DAYS Deposited 1997-12-02 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–125（104 aa）
Not recorded CA CALCIUM ION × 1 SGP GUANOSINE-2',3'-CYCLOPHOSPHOROTHIOATE × 1 3GP GUANOSINE-3'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；20 MM NAAC PH 4.2 2 MM CACL2 55 % MPD
Resolution 1.90 Å R-free 0.209
3HOH RIBONUCLEASE T1 (THR93GLN MUTANT) COMPLEXED WITH 2'GMP Deposited 1998-09-11 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：T93Q 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；25 MM NAAC PH 4.2 6.25 MM CACL2 42.5 % MPD
Resolution 1.95 Å R-free 0.213
3HOH RIBONUCLEASE T1 (THR93GLN MUTANT) COMPLEXED WITH 2'GMP Deposited 1998-09-11 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 27–130（104 aa）
Mutation：T93Q 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；25 MM NAAC PH 4.2 6.25 MM CACL2 42.5 % MPD
Resolution 1.95 Å R-free 0.213
3HOH RIBONUCLEASE T1 (THR93GLN MUTANT) COMPLEXED WITH 2'GMP Deposited 1998-09-11 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 27–130（104 aa）
Mutation：T93Q 2GP GUANOSINE-2'-MONOPHOSPHATE × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；25 MM NAAC PH 4.2 6.25 MM CACL2 42.5 % MPD
Resolution 1.95 Å R-free 0.213
3HOH RIBONUCLEASE T1 (THR93GLN MUTANT) COMPLEXED WITH 2'GMP Deposited 1998-09-11 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 27–130（104 aa）
Mutation：T93Q 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；25 MM NAAC PH 4.2 6.25 MM CACL2 42.5 % MPD
Resolution 1.95 Å R-free 0.213
3RNT CRYSTAL STRUCTURE OF GUANOSINE-FREE RIBONUCLEASE T1, COMPLEXED WITH VANADATE(V), SUGGESTS CONFORMATIONAL CHANGE UPON SUBSTRATE BINDING Deposited 1989-05-31 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–125（104 aa）
Not recorded CA CALCIUM ION × 1 VO4 VANADATE ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å
3URP Re-refinement of PDB entry 5RNT - ribonuclease T1 with guanosine-3',5'-diphosphate and phosphate ion bound Deposited 2011-11-22 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Not recorded PGP GUANOSINE-3',5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 UNX UNKNOWN LIGAND × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.19 Å R-free 0.237
4BIR RIBONUCLEASE T1: FREE HIS92GLN MUTANT Deposited 1998-01-13 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：H92Q CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；pH 4.2
Resolution 1.70 Å
4BU4 RIBONUCLEASE T1 COMPLEX WITH 2'GMP Deposited 1998-09-14 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Not recorded CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；20 MM NA-ACETATE PH 4.2 2 MM CACL2 55 % MPD
Resolution 1.80 Å R-free 0.253
4GSP RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS + 3'-GMP, 7 DAYS Deposited 1997-12-02 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–125（104 aa）
Not recorded CA CALCIUM ION × 1 3GP GUANOSINE-3'-MONOPHOSPHATE × 1 SGP GUANOSINE-2',3'-CYCLOPHOSPHOROTHIOATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；20 MM NAAC PH 4.2 2 MM CACL2 55 % MPD
Resolution 1.65 Å R-free 0.197
4HOH RIBONUCLEASE T1 (THR93ALA MUTANT) COMPLEXED WITH 2'GMP Deposited 1998-09-14 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：T93A 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；pH 4.20
Resolution 2.05 Å R-free 0.234
4HOH RIBONUCLEASE T1 (THR93ALA MUTANT) COMPLEXED WITH 2'GMP Deposited 1998-09-14 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 27–130（104 aa）
Mutation：T93A 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；pH 4.20
Resolution 2.05 Å R-free 0.234
4HOH RIBONUCLEASE T1 (THR93ALA MUTANT) COMPLEXED WITH 2'GMP Deposited 1998-09-14 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 27–130（104 aa）
Mutation：T93A 2GP GUANOSINE-2'-MONOPHOSPHATE × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；pH 4.20
Resolution 2.05 Å R-free 0.234
4HOH RIBONUCLEASE T1 (THR93ALA MUTANT) COMPLEXED WITH 2'GMP Deposited 1998-09-14 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 27–130（104 aa）
Mutation：T93A 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；pH 4.20
Resolution 2.05 Å R-free 0.234
4ODK Structure of SlyD from Thermus thermophilus in complex with T1 peptide Deposited 2014-01-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 59–73（15 aa） Fragment：T1 peptide (UNP residues 59-73)
Chain C 59–73（15 aa） Fragment：T1 peptide (UNP residues 59-73)
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；13.2% PEG1500, 0.1 M HEPES, pH 7.5, 0.05 M sodium chloride, 11% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 1.40 Å R-free 0.167
4RNT HIS 92 ALA MUTATION IN RIBONUCLEASE T1 INDUCES SEGMENTAL FLEXIBILITY. AN X-RAY STUDY Deposited 1990-02-13 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.20 Å
5BIR DISECTING HISTIDINE INTERACTIONS IN RIBONUCLEASE T1 USING ASN AND GLN MUTATIONS Deposited 1997-06-30 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：H92Q 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；VAPOR DIFFUSION, HANGING DROP, 20 MG/ML PROTEIN NAOAC BUF. PH 4.2, 0.125 % 2'GMP, 1.25 % CACL2, 47.5 % MPD, vapor diffusion - hanging drop
Resolution 2.00 Å R-free 0.255
5BIR DISECTING HISTIDINE INTERACTIONS IN RIBONUCLEASE T1 USING ASN AND GLN MUTATIONS Deposited 1997-06-30 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 27–130（104 aa）
Mutation：H92Q 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；VAPOR DIFFUSION, HANGING DROP, 20 MG/ML PROTEIN NAOAC BUF. PH 4.2, 0.125 % 2'GMP, 1.25 % CACL2, 47.5 % MPD, vapor diffusion - hanging drop
Resolution 2.00 Å R-free 0.255
5BIR DISECTING HISTIDINE INTERACTIONS IN RIBONUCLEASE T1 USING ASN AND GLN MUTATIONS Deposited 1997-06-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 27–130（104 aa）
Mutation：H92Q 2GP GUANOSINE-2'-MONOPHOSPHATE × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；VAPOR DIFFUSION, HANGING DROP, 20 MG/ML PROTEIN NAOAC BUF. PH 4.2, 0.125 % 2'GMP, 1.25 % CACL2, 47.5 % MPD, vapor diffusion - hanging drop
Resolution 2.00 Å R-free 0.255
5BU4 RIBONUCLEASE T1 COMPLEX WITH 2'GMP Deposited 1998-09-15 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Not recorded CA CALCIUM ION × 1 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；20 MM NA-ACETATE PH 4.2 2 MM CACL2 55 % MPD
Resolution 1.77 Å R-free 0.225
5GSP RIBONUCLEASE T1/3'-GMP, 9 WEEKS Deposited 1997-12-09 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–125（104 aa）
Not recorded CA CALCIUM ION × 1 3GP GUANOSINE-3'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；20 MM NAAC PH 4.2 2 MM CACL2 55 % MPD
Resolution 1.80 Å R-free 0.202
5HOH RIBONUCLEASE T1 (ASN9ALA/THR93ALA DOUBLEMUTANT) COMPLEXED WITH 2'GMP Deposited 1998-09-14 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Mutation：N9A,T93A 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；25 MM NAAC PH 4.2 6.25 MM CACL2 40.0 % MPD, pH 7.5
Resolution 2.00 Å R-free 0.212
5HOH RIBONUCLEASE T1 (ASN9ALA/THR93ALA DOUBLEMUTANT) COMPLEXED WITH 2'GMP Deposited 1998-09-14 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 27–130（104 aa）
Mutation：N9A,T93A 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；25 MM NAAC PH 4.2 6.25 MM CACL2 40.0 % MPD, pH 7.5
Resolution 2.00 Å R-free 0.212
5HOH RIBONUCLEASE T1 (ASN9ALA/THR93ALA DOUBLEMUTANT) COMPLEXED WITH 2'GMP Deposited 1998-09-14 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 27–130（104 aa）
Mutation：N9A,T93A 2GP GUANOSINE-2'-MONOPHOSPHATE × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；25 MM NAAC PH 4.2 6.25 MM CACL2 40.0 % MPD, pH 7.5
Resolution 2.00 Å R-free 0.212
5HOH RIBONUCLEASE T1 (ASN9ALA/THR93ALA DOUBLEMUTANT) COMPLEXED WITH 2'GMP Deposited 1998-09-14 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 27–130（104 aa）
Mutation：N9A,T93A 2GP GUANOSINE-2'-MONOPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；25 MM NAAC PH 4.2 6.25 MM CACL2 40.0 % MPD, pH 7.5
Resolution 2.00 Å R-free 0.212
5RNT X-RAY ANALYSIS OF CUBIC CRYSTALS OF THE COMPLEX FORMED BETWEEN RIBONUCLEASE T1 AND GUANOSINE-3',5'-BISPHOSPHATE Deposited 1991-03-28 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–125（104 aa）
Not recorded PGP GUANOSINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.20 Å
6GSP RIBONUCLEASE T1/3'-GMP, 15 WEEKS Deposited 1997-12-09 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–125（104 aa）
Not recorded CA CALCIUM ION × 1 3GP GUANOSINE-3'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；20 MM NAAC PH 4.2 2 MM CACL2 55 % MPD
Resolution 2.20 Å R-free 0.217
6RNT CRYSTAL STRUCTURE OF RIBONUCLEASE T1 COMPLEXED WITH ADENOSINE 2'-MONOPHOSPHATE AT 1.8-ANGSTROMS RESOLUTION Deposited 1991-08-20 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–125（104 aa）
Not recorded CA CALCIUM ION × 1 2AM ADENOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å
7GSP RIBONUCLEASE T1/2',3'-CGPS, NON-PRODUCTIVE Deposited 1997-12-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–125（104 aa）
Not recorded PO4 PHOSPHATE ION × 1 SGP GUANOSINE-2',3'-CYCLOPHOSPHOROTHIOATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；20 MM NAAC PH 4.2 2 MM CACL2 55 % MPD
Resolution 2.00 Å R-free 0.228
7GSP RIBONUCLEASE T1/2',3'-CGPS, NON-PRODUCTIVE Deposited 1997-12-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 22–125（104 aa）
Not recorded PO4 PHOSPHATE ION × 1 SGP GUANOSINE-2',3'-CYCLOPHOSPHOROTHIOATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；20 MM NAAC PH 4.2 2 MM CACL2 55 % MPD
Resolution 2.00 Å R-free 0.228
7RNT CRYSTAL STRUCTURE OF THE TYR45TRP MUTANT OF RIBONUCLEASE T1 IN A COMPLEX WITH 2'-ADENYLIC ACID Deposited 1991-08-20 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–130（104 aa）
Not recorded CA CALCIUM ION × 1 2AM ADENOSINE-2'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å
8RNT STRUCTURE OF RIBONUCLEASE T1 COMPLEXED WITH ZINC(II) AT 1.8 ANGSTROMS RESOLUTION: A ZN2+.6H2O.CARBOXYLATE CLATHRATE Deposited 1991-09-23 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–125（104 aa）
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å
9RNT RIBONUCLEASE T1 WITH FREE RECOGNITION AND CATALYTIC SITE: CRYSTAL STRUCTURE ANALYSIS AT 1.5 ANGSTROMS RESOLUTION Deposited 1991-09-25 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–125（104 aa）
Not recorded CA CALCIUM ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.50 Å

67 other PDB entries and 100 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RNT1_ASPOR
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–104； UniProt 27–130

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

暂无 SAXS 图

P(r) Distance Distribution P(r) Distribution

暂无 P(r) 图
Download Download
▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id3syu
Deposition date deposition_date2011-07-18
Structure title titleRe-refined coordinates for pdb entry 1det - ribonuclease T1 carboxymethylated at GLU 58 in complex with 2'GMP
Keywords keywordsHYDROLASE, ENDORIBONUCLEASE; HYDROLASE
Experimental Method methodX-RAY DIFFRACTION
▼

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

该条目暂无 SAXS 数据。

▼

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

该条目暂无 P(r) 分析数据。

▶

5. Crystallography and Experiment 5. Crystallography & Experiment

▶

6. Entities and Polymers Entities & Polymers (5)

▼

7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

Domain ID domain_idd3syua_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.1 — Microbial ribonucleases
Superfamily Superfamily superfamilyd.1.1 — Microbial ribonucleases
Family Family familyd.1.1.4 — Fungal ribonucleases

CATH v4.4 (1 domains)

Domain ID domain_id3syuA00
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology450 — Nuclear Transport Factor 2; Chain: A,
Homologous superfamily homologous superfamily30 — Microbial ribonucleases
▶

8. Citations (2)

▶

9. Files and Curves (0)