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4bio

FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH 8-HYDROXYQUINOLINE-5- CARBOXYLIC ACID

Method: X-RAY DIFFRACTION Dmax: 71.4 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

HYPOXIA-INDUCIBLE FACTOR 1-ALPHA INHIBITOR

HOMO SAPIENS

UniProt Q9NWT6

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 1–349 Not recorded FE FE (III) ION × 2 8XQ 8-hydroxyquinoline-5-carboxylic acid × 2 SO4 SULFATE ION × 10 GOL GLYCEROL × 8 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；1.2M AMMONIUM SULPHATE, 3% PEG400, PEG400, 0.001M FECL2, 0.1M HEPES PH7.5, VAPOR DIFFUSION, SITTING DROP Resolution 2.45 Å R-free 0.234

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4BIO Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1H2N Factor Inhibiting HIF-1 alpha Deposited 2002-08-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded FE2 FE (II) ION × 2 AKG 2-OXOGLUTARIC ACID × 2 SO4 SULFATE ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；1.2M AMMONIUM SULPHATE, 4% PEG400, 0.1M HEPES PH7.5 ARGON ATMOSPHERE, 11MG/ML PROTEIN WITH 1MM FE(II), 2.5MM AKG AND 2.5MM PEPTIDE (SEE REMARK 400), pH 7.50
Resolution 2.84 Å R-free 0.257
1IZ3 Dimeric structure of FIH (Factor inhibiting HIF) Deposited 2002-09-19 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；0.4M Lithium sulfate, 20% PEG 4000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.80 Å R-free 0.277
1MZE Human Factor Inhibiting HIF (FIH1) Deposited 2002-10-07 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded FE2 FE (II) ION × 2 TAR D(-)-TARTARIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9.6；294 K；potassium tartrate, CAPSO, glycerol, pH 9.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K
Resolution 2.20 Å R-free 0.237
1MZE Human Factor Inhibiting HIF (FIH1) Deposited 2002-10-07 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded FE2 FE (II) ION × 2 TAR D(-)-TARTARIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9.6；294 K；potassium tartrate, CAPSO, glycerol, pH 9.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K
Resolution 2.20 Å R-free 0.237
1MZF Human Factor inhibiting HIF (FIH1) in Complex with 2-oxoglutarate Deposited 2002-10-07 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded FE2 FE (II) ION × 2 AKG 2-OXOGLUTARIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9.6；294 K；potassium tartrate, CAPSO, iron(II) sulfate, 2-oxoglutarate, glycerol, pH 9.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K
Resolution 2.40 Å R-free 0.244
1MZF Human Factor inhibiting HIF (FIH1) in Complex with 2-oxoglutarate Deposited 2002-10-07 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded FE2 FE (II) ION × 2 AKG 2-OXOGLUTARIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9.6；294 K；potassium tartrate, CAPSO, iron(II) sulfate, 2-oxoglutarate, glycerol, pH 9.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K
Resolution 2.40 Å R-free 0.244
1YCI Factor inhibiting HIF-1 alpha in complex with N-(carboxycarbonyl)-D-phenylalanine Deposited 2004-12-22 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded FE2 FE (II) ION × 2 SO4 SULFATE ION × 6 NDF N-(CARBOXYCARBONYL)-D-PHENYLALANINE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；1.2M AMMONIUM SULPHATE, 4% PEG400, 0.1M HEPES PH7.5 ARGON ATMOSPHERE, 28MG/ML PROTEIN WITH 1MM FE(II), 10MM N-(CARBOXYCARBONYL)-D-PHENYLALANINE, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.70 Å R-free 0.244
2CGN FACTOR INHIBITING HIF-1 ALPHA with succinate Deposited 2006-03-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–349（349 aa）
Not recorded FE FE (III) ION × 1 SIN SUCCINIC ACID × 1 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；1.25M AMMONIUM SULFATE, 100MM HEPES PH7.5, 6% PEG400, 1MM IRON (II) SULFATE, 20MM SUCCINIC ACID, pH 7.50
Resolution 2.40 Å R-free 0.263
2CGO FACTOR INHIBITING HIF-1 ALPHA with fumarate Deposited 2006-03-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–349（349 aa）
Not recorded FE FE (III) ION × 1 FUM FUMARIC ACID × 1 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；1.25M AMMONIUM SULFATE, 100MM HEPES PH7.5, 6% PEG400, 1MM IRON (II) SULFATE, 20MM FUMARIC ACID, pH 7.50
Resolution 2.30 Å R-free 0.237
2ILM Factor Inhibiting HIF-1 Alpha D201A Mutant in Complex with FE(II), Alpha-Ketoglutarate and HIF-1 Alpha 35mer Deposited 2006-10-03 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–349（349 aa）
Mutation：D201A FE2 FE (II) ION × 2 SO4 SULFATE ION × 4 BCT BICARBONATE ION × 2 AKG 2-OXOGLUTARIC ACID × 2 GOL GLYCEROL × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；1.6M AMMONIUM SULPHATE, 6% PEG400, 0.1M HEPES PH7.5 ARGON ATMOSPHERE, 14MG/ML PROTEIN WITH 1MM FE(II)SO4, 1MM ALPHA-KETOGLUTARATE, 1MM HIF-1ALPHA PEPTIDE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298.0K, pH 7.50
Resolution 2.30 Å R-free 0.267
2W0X FACTOR INHIBITING HIF-1 ALPHA WITH PYRIDINE 2,4 DICARBOXYLIC ACID Deposited 2008-10-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded FE2 FE (II) ION × 2 PD2 PYRIDINE-2,4-DICARBOXYLIC ACID × 2 SO4 SULFATE ION × 6 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；1.6M AMMONIUM SULFATE, 0.1M HEPES PH 7.5, 5% PEG400, 1MM FESO4, 2MM SUBSTRATE
Resolution 2.12 Å R-free 0.262
2WA3 FACTOR INHIBITING HIF-1 ALPHA WITH 2-(3-hydroxyphenyl)-2-oxoacetic acid Deposited 2009-02-02 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded FE2 FE (II) ION × 2 A29 2-(3-HYDROXYPHENYL)-2-OXO-ETHANOIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；1.5 M AMMONIUM SULFATE, 100MM HEPES PH 7.5, 3% PEG400, 1MM FESO4, 28MG/ML PROTEIN, 10MM SUBSTRATE
Resolution 2.50 Å R-free 0.246
2WA4 FACTOR INHIBITING HIF-1 ALPHA WITH N,3-dihydroxybenzamide Deposited 2009-02-02 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded FE2 FE (II) ION × 2 069 N,3-DIHYDROXYBENZAMIDE × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；1.5M AMMONIUM SULFATE, 100MM HEPES PH 7.5, 3% PEG400, 1MM FESO4, 28MB/ML PROTEIN, 10MM SUBSTRATE. CRYSTALS ARE DEEP RED.
Resolution 2.50 Å R-free 0.267
2XUM FACTOR INHIBITING HIF (FIH) Q239H MUTANT IN COMPLEX WITH ZN(II), NOG AND ASP-SUBSTRATE PEPTIDE (20-MER) Deposited 2010-10-19 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–349（349 aa）
Mutation：YES ZN ZINC ION × 2 OGA N-OXALYLGLYCINE × 2 SO4 SULFATE ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；1.6M AMMONIUM SULPHATE, 6% PEG400, 0.1M HEPES PH7.5, VAPOR DIFFUSION, SITTING DROP
Resolution 2.20 Å R-free 0.222
2XUM FACTOR INHIBITING HIF (FIH) Q239H MUTANT IN COMPLEX WITH ZN(II), NOG AND ASP-SUBSTRATE PEPTIDE (20-MER) Deposited 2010-10-19 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Mutation：YES ZN ZINC ION × 1 OGA N-OXALYLGLYCINE × 1 SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；1.6M AMMONIUM SULPHATE, 6% PEG400, 0.1M HEPES PH7.5, VAPOR DIFFUSION, SITTING DROP
Resolution 2.20 Å R-free 0.222
2Y0I FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH TANKYRASE-2 (TNKS2) FRAGMENT PEPTIDE (21-MER) Deposited 2010-12-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–349（349 aa）
Not recorded FE2 FE (II) ION × 2 AKG 2-OXOGLUTARIC ACID × 2 SO4 SULFATE ION × 6 GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES PH 7.5 .
Resolution 2.28 Å R-free 0.248
2YC0 FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH R-2-HYDROXYGLUTARATE Deposited 2011-03-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded FE2 FE (II) ION × 2 2HG (2R)-2-hydroxypentanedioic acid × 2 GOL GLYCEROL × 6 SO4 SULFATE ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1M HEPES PH7.5, 1.6M AMMONIUM SULFATE, 6% PEG400, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K
Resolution 2.15 Å R-free 0.253
2YDE FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH S-2-HYDROXYGLUTARATE Deposited 2011-03-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded FE FE (III) ION × 2 S2G (2S)-2-HYDROXYPENTANEDIOIC ACID × 4 GOL GLYCEROL × 4 SO4 SULFATE ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1M HEPES PH7.5, 1.6M AMMONIUM SULPHATE, 6% PEG400, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K
Resolution 2.28 Å R-free 0.243
3D8C Factor inhibiting HIF-1 alpha D201G mutant in complex with ZN(II), alpha-ketoglutarate and HIF-1 alpha 19mer Deposited 2008-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 11–349（339 aa）
Mutation：D201G ZN ZINC ION × 2 SO4 SULFATE ION × 4 AKG 2-OXOGLUTARIC ACID × 2 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；1.6M AMMONIUM SULPHATE, 6% PEG400, 0.1M HEPES PH7.5, 24MG/ML PROTEIN WITH 1MM FE(II)SO4, 1MM ALPHA-KETOGLUTARATE, 1MM HIF-1ALPHA PEPTIDE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298.0K, PH 7.50
Resolution 2.10 Å R-free 0.256
3D8C Factor inhibiting HIF-1 alpha D201G mutant in complex with ZN(II), alpha-ketoglutarate and HIF-1 alpha 19mer Deposited 2008-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 11–349（339 aa）
Mutation：D201G ZN ZINC ION × 1 SO4 SULFATE ION × 2 AKG 2-OXOGLUTARIC ACID × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；1.6M AMMONIUM SULPHATE, 6% PEG400, 0.1M HEPES PH7.5, 24MG/ML PROTEIN WITH 1MM FE(II)SO4, 1MM ALPHA-KETOGLUTARATE, 1MM HIF-1ALPHA PEPTIDE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298.0K, PH 7.50
Resolution 2.10 Å R-free 0.256
3KCX Factor inhibiting HIF-1 alpha in complex with Clioquinol Deposited 2009-10-22 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 15–349（335 aa） Fragment：residues 15-349
Not recorded FE2 FE (II) ION × 2 SO4 SULFATE ION × 4 GOL GLYCEROL × 6 CQL 5-chloro-7-iodoquinolin-8-ol × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；1.6M ammonium sulfate, 2% polyethyleneglycol 400, 0.1M Tris-HCl, pH 7.5, vapor diffusion, hanging drop, temperature 293K
Resolution 2.60 Å R-free 0.292
3KCY Factor inhibiting HIF-1 alpha in complex with 8-hydroxyquinoline Deposited 2009-10-22 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 15–349（335 aa） Fragment：residues 15-349
Not recorded FE2 FE (II) ION × 2 SO4 SULFATE ION × 4 HQY quinolin-8-ol × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；1.6M ammonium sulfate, 2% polyethyleneglycol 400, 0.1M Tris-HCl, pH 7.5, vapor diffusion, hanging drop, temperature 293K
Resolution 2.59 Å R-free 0.335
3OD4 Crystal Structure of Factor Inhibiting HIF-1 Alpha Complexed with Inhibitor Deposited 2010-08-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded ZN ZINC ION × 2 8XQ 8-hydroxyquinoline-5-carboxylic acid × 2 SO4 SULFATE ION × 6 GOL GLYCEROL × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；1.75M AMMONIUM SULFATE, 0.1M HEPES PH 7.5, 2% PEG 400, 2MM ZNCL2, 0.1MM LIGAND , VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.20 Å R-free 0.212
3P3N Factor inhibiting HIF-1 Alpha in complex with Notch 1 fragment mouse notch (1930-1949) peptide Deposited 2010-10-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–349（349 aa）
Not recorded FE2 FE (II) ION × 2 SO4 SULFATE ION × 4 AKG 2-OXOGLUTARIC ACID × 2 GOL GLYCEROL × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；1.6M AMMONIUM SULFATE, 4% PEG400, 0.1M HEPES PH7.5, NITROGEN ATMOSPHERE, 20MG/ML PROTEIN WITH 1MM FE(II), 2.0MM 2OG AND 10MM PEPTIDE , VAPOR DIFFUSION, SITTING DROP, temperature 298.0K
Resolution 2.40 Å R-free 0.244
3P3P Factor inhibiting HIF-1 Alpha in complex with Notch 1 fragment mouse notch (1997-2016) peptide Deposited 2010-10-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–349（349 aa）
Not recorded FE2 FE (II) ION × 2 AKG 2-OXOGLUTARIC ACID × 2 SO4 SULFATE ION × 4 GOL GLYCEROL × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；1.6M AMMONIUM SULFATE, 4% PEG400, 0.1M HEPES PH7.5, NITROGEN ATMOSPHERE, 20MG/ML PROTEIN WITH 1MM FE(II), 2.0MM 2OG AND 10MM PEPTIDE , VAPOR DIFFUSION, SITTING DROP, temperature 298.0K
Resolution 2.60 Å R-free 0.259
4AI8 FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH DAMINOZIDE Deposited 2012-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded SO4 SULFATE ION × 10 GOL GLYCEROL × 6 DZA DAMINOZIDE × 2 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES PH7.5, 1.6 M AMMONIUM SULFATE, 6% PEG400, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K
Resolution 2.40 Å R-free 0.248
4B7E FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH CONSENSUS ANKYRIN REPEAT DOMAIN-LEU PEPTIDE (20-MER) Deposited 2012-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–349（349 aa）
Not recorded ZN ZINC ION × 2 OGA N-OXALYLGLYCINE × 2 SO4 SULFATE ION × 10 GOL GLYCEROL × 8 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；1.6M AMMONIUM SULPHATE, 6% PEG400, 0.1M HEPES PH 7.5
Resolution 2.50 Å R-free 0.255
4B7K FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH CONSENSUS ANKYRIN REPEAT DOMAIN-SER PEPTIDE (20-MER) Deposited 2012-08-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–349（349 aa）
Not recorded ZN ZINC ION × 2 OGA N-OXALYLGLYCINE × 2 SO4 SULFATE ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；1.6 M AMMONIUM SULPHATE, 6% PEG 400, 0.1 M HEPES PH 7.5
Resolution 2.39 Å R-free 0.241
4JAA Factor inhibiting HIF-1 alpha in complex with consensus ankyrin repeat domain-(d)LEU peptide Deposited 2013-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded ZN ZINC ION × 1 OGA N-OXALYLGLYCINE × 1 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES PH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.39 Å R-free 0.219
4NR1 Factor inhibiting HIF-1 alpha in complex with consensus ankyrin repeat domain-(d)allyl-GLY peptide Deposited 2013-11-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded ZN ZINC ION × 1 OGA N-OXALYLGLYCINE × 1 SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.68 Å R-free 0.210
4Z1V Structure of Factor Inhibiting HIF (FIH) in complex with Fe, NO, and NOG Deposited 2015-03-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded OGA N-OXALYLGLYCINE × 2 NO NITRIC OXIDE × 2 PEG DI(HYDROXYETHYL)ETHER × 2 FE FE (III) ION × 2 SO4 SULFATE ION × 10 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；0.1M Hepes, 1.6mM ammonium sulfate, 3% PEG 400
Resolution 2.10 Å R-free 0.240
4Z2W Factor Inhibiting HIF in Complex with Fe, and Alpha-Ketoglutarate Deposited 2015-03-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded AKG 2-OXOGLUTARIC ACID × 2 PEG DI(HYDROXYETHYL)ETHER × 2 FE FE (III) ION × 2 SO4 SULFATE ION × 10 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；0.1M Hepes, 1.6mM ammonium sulfate, 3% PEG 400
Resolution 2.50 Å R-free 0.246
5JWK Factor Inhibiting HIF D201E in Complex with Fe, and Alpha-Ketoglutarate Deposited 2016-05-12 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Mutation：D201E AKG 2-OXOGLUTARIC ACID × 2 PEG DI(HYDROXYETHYL)ETHER × 2 FE2 FE (II) ION × 2 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M Hepes, 1.6mM ammonium sulfate, 3% PEG 400
Resolution 2.30 Å R-free 0.227
5JWL Factor Inhibiting HIF D201E in Complex with Zn, and Alpha-Ketoglutarate Deposited 2016-05-12 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Mutation：D201E AKG 2-OXOGLUTARIC ACID × 2 PEG DI(HYDROXYETHYL)ETHER × 2 ZN ZINC ION × 2 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M Hepes, 1.6mM ammonium sulfate, 3% PEG 400
Resolution 2.40 Å R-free 0.226
5JWP Crystal structure of human FIH D201E variant in complex with Zn, alpha-ketoglutarate, and HIF1 alpha peptide. Deposited 2016-05-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–349（349 aa）
Mutation：D201E AKG 2-OXOGLUTARIC ACID × 2 GOL GLYCEROL × 2 ZN ZINC ION × 2 SO4 SULFATE ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M Hepes, 1.6mM ammonium sulfate, 3% PEG 400
Resolution 2.10 Å R-free 0.209
5OP6 Factor Inhibiting HIF (FIH) in complex with zinc and GSK128863 Deposited 2017-08-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–349（349 aa）
Not recorded ZN ZINC ION × 1 SO4 SULFATE ION × 2 A0W 2-[[1,3-dicyclohexyl-4-oxidanyl-2,6-bis(oxidanylidene)pyrimidin-5-yl]carbonylamino]ethanoic acid × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；300 K；0.1M HEPES pH7.5, 1.1M ammonium sulfate, 2.5% PEG 400
Resolution 2.45 Å R-free 0.227
5OP8 Factor Inhibiting HIF (FIH) in complex with zinc and Molidustat Deposited 2017-08-09 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded ZN ZINC ION × 2 A1H 2-(6-morpholin-4-ylpyrimidin-4-yl)-4-(1,2,3-triazol-1-yl)-1~{H}-pyrazol-3-one × 2 SO4 SULFATE ION × 6 GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；300 K；0.1M HEPES pH7.5, 1.4M ammonium sulfate, 4.5% PEG400
Resolution 2.30 Å R-free 0.214
5OPC Factor Inhibiting HIF (FIH) in complex with zinc and Vadadustat Deposited 2017-08-09 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded SO4 SULFATE ION × 10 ZN ZINC ION × 2 GOL GLYCEROL × 10 A1Z Vadadustat × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；300 K；0.1M HEPES pH7.5, 1.7M ammonium sulfate, 5.5% PEG 400
Resolution 2.30 Å R-free 0.214
6H9J Factor Inhibiting HIF (FIH) in complex with zinc, NOG and TRPV3 (229-255) Deposited 2018-08-04 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 12–349（338 aa）
Not recorded OGA N-OXALYLGLYCINE × 2 GOL GLYCEROL × 6 SO4 SULFATE ION × 8 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M Hepes pH = 7.5, 1.8 M ammonium sulfate, 4.5% PEG400
Resolution 1.83 Å R-free 0.187
6HA6 Factor Inhibiting HIF (FIH) in complex with zinc, NOG and TRPV3 (220-246) Deposited 2018-08-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–349（349 aa）
Not recorded OGA N-OXALYLGLYCINE × 2 SO4 SULFATE ION × 8 GOL GLYCEROL × 6 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M Hepes pH = 7.5, 1.7 M ammonium sulfate, 5.5% PEG400
Resolution 1.98 Å R-free 0.203
6HC8 Factor Inhibiting HIF (FIH) in complex with zinc, NOG and TRPA1 (313-339) Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–349（349 aa）
Not recorded ZN ZINC ION × 2 OGA N-OXALYLGLYCINE × 2 SO4 SULFATE ION × 2 GOL GLYCEROL × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M Hepes pH = 7.5, 1.6 M ammonium sulfate, 6% PEG400
Resolution 1.90 Å R-free 0.189
6HKP Factor Inhibiting HIF (FIH) in complex with zinc, NOG and ASPP2 (970-992) Deposited 2018-09-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–349（349 aa）
Not recorded SO4 SULFATE ION × 12 GOL GLYCEROL × 10 OGA N-OXALYLGLYCINE × 2 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M Hepes pH = 7.5, 2.0 M ammonium sulfate, 5.5% PEG400
Resolution 1.90 Å R-free 0.195
6HL5 Factor Inhibiting HIF (FIH) in complex with zinc, NOG and ASPP1(932-954) Deposited 2018-09-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–349（349 aa）
Not recorded GOL GLYCEROL × 8 SO4 SULFATE ION × 8 ZN ZINC ION × 2 OGA N-OXALYLGLYCINE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M Hepes pH = 7.5, 1.2 M ammonium sulfate, 4.5% PEG400
Resolution 1.98 Å R-free 0.201
6HL6 Factor Inhibiting HIF (FIH) in complex with zinc, NOG and iASPP(670-693) Deposited 2018-09-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–349（349 aa）
Not recorded GOL GLYCEROL × 10 SO4 SULFATE ION × 6 ZN ZINC ION × 2 OGA N-OXALYLGLYCINE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；273 K；0.1 M Hepes pH = 7.5, 1.6 M ammonium sulfate, 3.5% PEG400
Resolution 1.97 Å R-free 0.205
6RUJ Factor inhibiting HIF-1 alpha in complex with consensus ankyrin repeat domain-(d)3-hydroxy-Leu peptide Deposited 2019-05-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–349（349 aa）
Not recorded ZN ZINC ION × 2 OGA N-OXALYLGLYCINE × 2 SO4 SULFATE ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.42 Å R-free 0.257
7A1J FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH ZN(II) AND 3-(3-phenylpropyl)-2-oxoglutarate Deposited 2020-08-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded ZN ZINC ION × 2 SO4 SULFATE ION × 10 QVN 3-(3-phenylpropyl)-2-oxoglutarate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES PH 7.5
Resolution 1.90 Å R-free 0.209
7A1K FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH ZN(II) AND 3-((9,9-dimethyl-9H-fluoren-2-yl)methyl)-2-oxoglutarate Deposited 2020-08-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded ZN ZINC ION × 2 QVZ 3-((9,9-dimethyl-9H-fluoren-2-yl)methyl)-2-oxoglutarate × 2 SO4 SULFATE ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES PH 7.5
Resolution 1.99 Å R-free 0.227
7A1L FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH ZN(II) AND 3-methyl-2-oxoglutarate Deposited 2020-08-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–349（349 aa）
Not recorded ROQ (3~{S})-3-methyl-2-oxidanylidene-pentanedioic acid × 1 ZN ZINC ION × 1 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES PH 7.5
Resolution 2.29 Å R-free 0.222
7A1M FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH ZN(II) AND 3-propyl-2-oxoglutarate Deposited 2020-08-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded ZN ZINC ION × 2 QVW 3-propyl-2-oxoglutarate × 2 SO4 SULFATE ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES PH 7.5
Resolution 2.18 Å R-free 0.245
7A1N FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH ZN(II), 3-methyl-2-oxoglutarate, AND CONSENSUS ANKYRIN REPEAT DOMAIN (20-MER) Deposited 2020-08-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded SO4 SULFATE ION × 9 ZN ZINC ION × 1 ROQ (3~{S})-3-methyl-2-oxidanylidene-pentanedioic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES PH 7.5
Resolution 2.01 Å R-free 0.234
7A1O FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH ZN(II), 4-ethyl-2-oxoglutarate, AND CONSENSUS ANKYRIN REPEAT DOMAIN (20-MER) Deposited 2020-08-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–349（349 aa）
Not recorded ZN ZINC ION × 2 SO4 SULFATE ION × 8 QVT 4-ethyl-2-oxoglutarate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES PH 7.5
Resolution 2.21 Å R-free 0.243
7A1P FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH ZN(II), 4-propyl-2-oxoglutarate, AND CONSENSUS ANKYRIN REPEAT DOMAIN (20-MER) Deposited 2020-08-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–349（349 aa）
Not recorded ZN ZINC ION × 2 SO4 SULFATE ION × 6 QW2 (4~{S})-2-oxidanylidene-4-propyl-pentanedioic acid × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES PH 7.5
Resolution 1.76 Å R-free 0.191
7A1Q FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH ZN(II), 3-(carboxycarbonyl)cyclopentane-1-carboxylic acid, AND CONSENSUS ANKYRIN REPEAT DOMAIN (20-MER) Deposited 2020-08-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–349（349 aa）
Not recorded ZN ZINC ION × 2 SO4 SULFATE ION × 6 QVQ 3-(carboxycarbonyl)cyclopentane-1-carboxylic acid × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES PH 7.5
Resolution 1.75 Å R-free 0.200
7A1S FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH ZN(II), 3-methyl-2-oxoglutarate, AND TANKYRASE-2 (TNKS2) FRAGMENT PEPTIDE (21-MER) Deposited 2020-08-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded SO4 SULFATE ION × 8 ROQ (3~{S})-3-methyl-2-oxidanylidene-pentanedioic acid × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES PH 7.5
Resolution 2.01 Å R-free 0.209
8IHZ FACTOR INHIBITING HIF-1 ALPHA in complex with (5-(1-(3-(4-chlorophenyl)propyl)-1H-1,2,3-triazol-4-yl)-3-hydroxypicolinoyl)glycine Deposited 2023-02-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–349（349 aa）
Not recorded SO4 SULFATE ION × 14 ZN ZINC ION × 1 P1X 2-[[5-[1-[3-(4-chlorophenyl)propyl]-1,2,3-triazol-4-yl]-3-oxidanyl-pyridin-2-yl]carbonylamino]ethanoic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；1.6 M AMMONIUM SULPHATE, 6% PEG 400, 0.1 M HEPES
Resolution 2.22 Å R-free 0.261
8II0 FACTOR INHIBITING HIF-1 ALPHA in complex with (5-(3-(3-chlorophenyl)isoxazol-5-yl)-3-hydroxypicolinoyl)glycine Deposited 2023-02-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–349（349 aa）
Not recorded P5I 2-[[5-[3-(3-chlorophenyl)-1,2-oxazol-5-yl]-3-oxidanyl-pyridin-2-yl]carbonylamino]ethanoic acid × 1 GOL GLYCEROL × 2 ZN ZINC ION × 1 SO4 SULFATE ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；1.6 M AMMONIUM SULPHATE, 6% PEG 400, 0.1 M HEPES.
Resolution 2.04 Å R-free 0.239
8K71 Factor-inhibiting hypoxia-inducible factor in complex with Zn(II) and 2-(3-hydroxy-2-((2-((naphthalen-2-ylmethyl)sulfonyl)acetyl)imino)-2,3-dihydrothiazol-4-yl)acetic acid Deposited 2023-07-26 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded ZN ZINC ION × 2 VIZ 2-[(2~{Z})-2-[2-(naphthalen-2-ylmethylsulfonyl)ethanoylimino]-3-oxidanyl-1,3-thiazol-4-yl]ethanoic acid × 2 SO4 SULFATE ION × 16 GOL GLYCEROL × 14 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；0.27 mM FIH, 0.5 mM zinc acetate, 2 mM BNS, 0.1 M HEPES, pH 7.5, 6%w/v PEG400, 1.6 M ammonium sulfate
Resolution 2.23 Å R-free 0.236
8K72 Factor-inhibiting hypoxia-inducible factor in complex with Zn(II) and 2-(3-hydroxy-2-((3-(phenylsulfonamido)propanoyl)imino)-2,3-dihydrothiazol-4-yl)acetic acid Deposited 2023-07-26 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded SO4 SULFATE ION × 20 GOL GLYCEROL × 6 ZN ZINC ION × 2 VJF 2-[(2~{Z})-3-oxidanyl-2-[3-(phenylsulfonylamino)propanoylimino]-1,3-thiazol-4-yl]ethanoic acid × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；0.27 mM FIH, 0.5 mM zinc acetate, 2 mM TC167, 0.1 M HEPES, pH 7.5, 6%w/v PEG400, 1.6 M ammonium sulfate
Resolution 2.45 Å R-free 0.239
8K73 Factor-inhibiting hypoxia-inducible factor in complex with Zn(II) and 2-(3-hydroxy-2-((1-(phenylsulfonyl)pyrrolidine-3-carbonyl)imino)-2,3-dihydrothiazol-4-yl)acetic acid Deposited 2023-07-26 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded SO4 SULFATE ION × 14 GOL GLYCEROL × 14 ZN ZINC ION × 2 VK6 2-[(2~{Z})-3-oxidanyl-2-[(3~{R})-1-(phenylsulfonyl)pyrrolidin-3-yl]carbonylimino-1,3-thiazol-4-yl]ethanoic acid × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；0.27 mM FIH, 0.5 mM zinc acetate, 2 mM RT105, 0.1 M HEPES, pH 7.5, 6%w/v PEG400, 1.6 M ammonium sulfate
Resolution 2.02 Å R-free 0.223
9FSN FIH in complex with Enarodustat crystal structure at 2.2A Deposited 2024-06-21 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded MN MANGANESE (II) ION × 2 A1IF7 Enarodustat × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；290 K；Morpheus 1, well C1, 0.09 M NPS, 0.1 M Buffer System 1 pH 6.5, 30 % Precipitant Mix 1
Resolution 2.20 Å R-free 0.208
9I4H Factor Inhibiting HIF (FIH) in complex with manganese and 3-Hydroxy-5-(3-(4-(hydroxymethyl)-3-nitrophenyl)isoxazol-5-yl)picolinoyl)glycine Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–349（349 aa）
Not recorded MN MANGANESE (II) ION × 2 A1IZ5 N-[(5P)-3-hydroxy-5-{(3P)-3-[4-(hydroxymethyl)-3-nitrophenyl]-1,2-oxazol-5-yl}pyridine-2-carbonyl]glycine × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；290 K；Morpheus C5 0.09 M NPS 0.1 M Buffer System 2 7.5 30 % v/v Precipitant Mix 2
Resolution 2.30 Å R-free 0.218
9IIF Factor inhibiting HIF-1 alpha in complex with Zn(II) and Desidustat Deposited 2024-06-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–349（349 aa）
Not recorded SO4 SULFATE ION × 8 A1L2L 2-[[1-(cyclopropylmethoxy)-2-oxidanyl-4-oxidanylidene-quinolin-3-yl]carbonylamino]ethanoic acid × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；0.27 mM FIH, 0.5 mM zinc acetate, 2 mM densidustat, 0.1 M HEPES, pH 7.5, 6% w/v PEG400, 1.6 M ammonium sulfate
Resolution 2.16 Å R-free 0.244
9JTX Factor inhibiting HIF-1 alpha in complex with Zn(II) and 2-(4-hydroxy-2-oxo-1-(thiazol-4-ylmethoxy)-1,2-dihydroquinoline-3-carboxamido)-2-methylpropanoic acid Deposited 2024-10-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–349（349 aa）
Not recorded SO4 SULFATE ION × 7 A1L4V 2-methyl-2-[[4-oxidanyl-2-oxidanylidene-1-(1,3-thiazol-4-ylmethoxy)quinolin-3-yl]carbonylamino]propanoic acid × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES, pH 7.5, 6%w/v PEG400, 1.6 M ammonium sulfate
Resolution 2.08 Å R-free 0.247
9M23 FIH [Factor Inhibiting HIF (Hypoxia Inducible Factor)] in complex with Zn(II) and N-((4-(trifluoromethyl)phenyl)sulfonyl)picolinamide (SB133) Deposited 2025-02-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–349（349 aa）
Not recorded GOL GLYCEROL × 1 ZN ZINC ION × 1 A1L8D ~{N}-[4-(trifluoromethyl)phenyl]sulfonylpyridine-2-carboxamide × 1 SO4 SULFATE ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES PH 7.5
Resolution 2.16 Å R-free 0.226

60 other PDB entries and 64 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name HIF1N_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 4–352； UniProt 1–349

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 4bio

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 4bio
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2. Structure Basics 2. Structure Basics

Entry ID entry_id4bio
Deposition date deposition_date2013-04-12
Structure title titleFACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH 8-HYDROXYQUINOLINE-5- CARBOXYLIC ACID
Keywords keywords;OXIDOREDUCTASE, DIOXYGENASE, OXYGENASE, DNA-BINDING, METAL-BINDING, TRANSCRIPTION, HELIX-LOOP-HELIX-BETA, DSBH, FACIAL TRIAD, ASPARAGINYL/ASPARTYL HYDROXYLASE, TRANSCRIPTION AND EPIGENETIC REGULATION, SIGNALING, DEVELOPMENT, CELL STRUCTURE, ANKYRIN REPEAT DOMAIN, ARD, BETA-HYDROXYLATION, TRANSCRIPTION ACTIVATOR/INHIBITOR, PHOSPHORYLATION, S-NITROSYLATION ;; OXIDOREDUCTASE
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier21.80 Å
Radius of gyration Rg (electron density) rg_electron20.61 Å
Forward intensity I(0) i028443100.00
Molecular weight molecular_weight40088.0 kDa
Excluded volume excluded_volume49735 ų
Envelope volume envelope_volume60228 ų
Hydration-shell volume shell_volume23930 ų
Envelope diameter envelope_diameter72.3 Å
Shell Rg shell_rg27.87 Å
Envelope Rg envelope_rg21.06 Å
Shape Rg shape_rg20.57 Å
Total Rg total_rg21.65 Å
Total atoms total_atoms2823
Residues n_residues339
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax71.4 Å
Rg (real space) rg_real21.69 Å
Rg uncertainty (real space) rg_real_error0.51 Å
I(0) (real space) i0_real2.8440e+07
I(0) uncertainty (real space) i0_real_error3.8840e+05
Rg (reciprocal space) rg_reciprocal21.71 Å
I(0) (reciprocal space) i0_reciprocal28440000.0000
Solution quality estimate total_estimate0.8806
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary27.7 Å
Skewness Skewness skewness0.238
Kurtosis Kurtosis kurtosis-0.257
Angular range angular_range— – 0.3650 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha6092000.0000
Real-space data points n_real_points68
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.817; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.994
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (6)

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7. Fold Classification (SCOP + CATH) 3 domains

SCOP 2.08 (1 domains)

Domain ID domain_idd4bioa_
Class classb — All beta proteins
Fold Fold foldb.82 — Double-stranded beta-helix
Superfamily Superfamily superfamilyb.82.2 — Clavaminate synthase-like
Family Family familyb.82.2.6 — Hypoxia-inducible factor HIF ihhibitor (FIH1)

CATH v4.4 (2 domains)

Domain ID domain_id4bioA01
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology120 — Jelly Rolls
Homologous superfamily homologous superfamily10 — Jelly Rolls
Domain ID domain_id4bioA02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology287 — Helix Hairpins
Homologous superfamily homologous superfamily1010 — Clavaminate synthase-like
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8. Citations (1)

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9. Files and Curves (10)