SAXSdb — 4irv

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

Cytotoxicity-associated immunodominant antigen

Helicobacter pylori

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白异源复合物异源复合物蛋白 × 2 PDB 声明：dimeric（2）与蛋白拷贝数一致	链 A； UniProt 19–235	非标准单体：是（mmCIF未提供具体位点）	Apoptosis-stimulating of p53 protein 2 × 1 （Q13625）	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 8.5；Crystals were grown by vapor diffusion at 25 degrees C using hanging drops formed from mixing a 2ul of the protein complex with 2ul of an equilibration buffer (21% polyethylene glycol (PEG) molecular weight 4 kDa, 200 mM Li2 SO4 , and 100 mM Tris pH 8.5) and 0.6ul of the Silver Bullets additive 43 (Hampton Research HR2-996-43). Significantly higher quality crystals were obtained from selenomethionine-substituted protein complexes and they were used for the final refinement, VAPOR DIFFUSION, HANGING DROP	分辨率 2.04 Å R-free 0.234
2	蛋白异源复合物异源复合物蛋白 × 2 PDB 声明：dimeric（2）与蛋白拷贝数一致	链 B； UniProt 19–235	非标准单体：是（mmCIF未提供具体位点）	Apoptosis-stimulating of p53 protein 2 × 1 （Q13625）	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 8.5；Crystals were grown by vapor diffusion at 25 degrees C using hanging drops formed from mixing a 2ul of the protein complex with 2ul of an equilibration buffer (21% polyethylene glycol (PEG) molecular weight 4 kDa, 200 mM Li2 SO4 , and 100 mM Tris pH 8.5) and 0.6ul of the Silver Bullets additive 43 (Hampton Research HR2-996-43). Significantly higher quality crystals were obtained from selenomethionine-substituted protein complexes and they were used for the final refinement, VAPOR DIFFUSION, HANGING DROP	分辨率 2.04 Å R-free 0.234
3	蛋白异源复合物异源复合物蛋白 × 2 PDB 声明：dimeric（2）与蛋白拷贝数一致	链 C； UniProt 19–235	非标准单体：是（mmCIF未提供具体位点）	Apoptosis-stimulating of p53 protein 2 × 1 （Q13625）	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 8.5；Crystals were grown by vapor diffusion at 25 degrees C using hanging drops formed from mixing a 2ul of the protein complex with 2ul of an equilibration buffer (21% polyethylene glycol (PEG) molecular weight 4 kDa, 200 mM Li2 SO4 , and 100 mM Tris pH 8.5) and 0.6ul of the Silver Bullets additive 43 (Hampton Research HR2-996-43). Significantly higher quality crystals were obtained from selenomethionine-substituted protein complexes and they were used for the final refinement, VAPOR DIFFUSION, HANGING DROP	分辨率 2.04 Å R-free 0.234
4	蛋白异源复合物异源复合物蛋白 × 2 PDB 声明：dimeric（2）与蛋白拷贝数一致	链 D； UniProt 19–235	非标准单体：是（mmCIF未提供具体位点）	Apoptosis-stimulating of p53 protein 2 × 1 （Q13625）	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 8.5；Crystals were grown by vapor diffusion at 25 degrees C using hanging drops formed from mixing a 2ul of the protein complex with 2ul of an equilibration buffer (21% polyethylene glycol (PEG) molecular weight 4 kDa, 200 mM Li2 SO4 , and 100 mM Tris pH 8.5) and 0.6ul of the Silver Bullets additive 43 (Hampton Research HR2-996-43). Significantly higher quality crystals were obtained from selenomethionine-substituted protein complexes and they were used for the final refinement, VAPOR DIFFUSION, HANGING DROP	分辨率 2.04 Å R-free 0.234

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly， “相对当前条目”直接指出证据层面的不同；没有差异标签表示当前已读取字段一致。

其他 PDB	相对当前条目 4IRV	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
3IEC Helicobacter pylori CagA Inhibits PAR1/MARK Family Kinases by Mimicking Host Substrates 提交 2009-07-22	构建体不同突变/修饰不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 E 885–1005（121 aa）片段：UNP RESIDUES 885-1005	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；296 K；Initial crystals were grown by vapor diffusion using hanging drops formed from mixing a 2:1 volume ratio of the protein complex with an equilibration buffer consisting of 22.5-25% polyethylene glycol (PEG) molecular weight 3350 Da, 300 mM Li2SO4, and 100 mM Bis-Tris pH 5.8-6.2, at 23 C. Higher quality crystals were obtained by micro-seeding and addition of 5% PEG 400 as an additive. For cryoprotection crystals where transferred directly into a buffer with a 25% PEG 3350 Da, 5-10% PEG 400 Da, 300mM Li2SO4, 100mM Bis-Tris pH 5.8-6.2, 7.5% glycerol, and flash-cooled immediately afterward to -160 C. , VAPOR DIFFUSION, HANGING DROP, temperature 296K	分辨率 2.20 Å R-free 0.248
3IEC Helicobacter pylori CagA Inhibits PAR1/MARK Family Kinases by Mimicking Host Substrates 提交 2009-07-22	构建体不同突变/修饰不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 F 885–1005（121 aa）片段：UNP RESIDUES 885-1005	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；296 K；Initial crystals were grown by vapor diffusion using hanging drops formed from mixing a 2:1 volume ratio of the protein complex with an equilibration buffer consisting of 22.5-25% polyethylene glycol (PEG) molecular weight 3350 Da, 300 mM Li2SO4, and 100 mM Bis-Tris pH 5.8-6.2, at 23 C. Higher quality crystals were obtained by micro-seeding and addition of 5% PEG 400 as an additive. For cryoprotection crystals where transferred directly into a buffer with a 25% PEG 3350 Da, 5-10% PEG 400 Da, 300mM Li2SO4, 100mM Bis-Tris pH 5.8-6.2, 7.5% glycerol, and flash-cooled immediately afterward to -160 C. , VAPOR DIFFUSION, HANGING DROP, temperature 296K	分辨率 2.20 Å R-free 0.248
3IEC Helicobacter pylori CagA Inhibits PAR1/MARK Family Kinases by Mimicking Host Substrates 提交 2009-07-22	构建体不同突变/修饰不同实验环境不同结构质量不同	Assembly 3 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 G 885–1005（121 aa）片段：UNP RESIDUES 885-1005	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；296 K；Initial crystals were grown by vapor diffusion using hanging drops formed from mixing a 2:1 volume ratio of the protein complex with an equilibration buffer consisting of 22.5-25% polyethylene glycol (PEG) molecular weight 3350 Da, 300 mM Li2SO4, and 100 mM Bis-Tris pH 5.8-6.2, at 23 C. Higher quality crystals were obtained by micro-seeding and addition of 5% PEG 400 as an additive. For cryoprotection crystals where transferred directly into a buffer with a 25% PEG 3350 Da, 5-10% PEG 400 Da, 300mM Li2SO4, 100mM Bis-Tris pH 5.8-6.2, 7.5% glycerol, and flash-cooled immediately afterward to -160 C. , VAPOR DIFFUSION, HANGING DROP, temperature 296K	分辨率 2.20 Å R-free 0.248
3IEC Helicobacter pylori CagA Inhibits PAR1/MARK Family Kinases by Mimicking Host Substrates 提交 2009-07-22	构建体不同突变/修饰不同实验环境不同结构质量不同	Assembly 4 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 H 885–1005（121 aa）片段：UNP RESIDUES 885-1005	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；296 K；Initial crystals were grown by vapor diffusion using hanging drops formed from mixing a 2:1 volume ratio of the protein complex with an equilibration buffer consisting of 22.5-25% polyethylene glycol (PEG) molecular weight 3350 Da, 300 mM Li2SO4, and 100 mM Bis-Tris pH 5.8-6.2, at 23 C. Higher quality crystals were obtained by micro-seeding and addition of 5% PEG 400 as an additive. For cryoprotection crystals where transferred directly into a buffer with a 25% PEG 3350 Da, 5-10% PEG 400 Da, 300mM Li2SO4, 100mM Bis-Tris pH 5.8-6.2, 7.5% glycerol, and flash-cooled immediately afterward to -160 C. , VAPOR DIFFUSION, HANGING DROP, temperature 296K	分辨率 2.20 Å R-free 0.248
4DVY Crystal structure of the Helicobacter pylori CagA oncoprotein 提交 2012-02-23	构建体不同突变/修饰不同聚集状态不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 P 1–876（876 aa）片段：UNP residues 1-876	突变：H131M/L274M	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；8% EtOH, 50mM Tris-HCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K	分辨率 3.30 Å R-free 0.248
4DVZ Crystal structure of the Helicobacter pylori CagA oncoprotein 提交 2012-02-23	构建体不同突变/修饰不同聚集状态不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 261–829（569 aa）片段：UNP residues 261-829	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；7% EtOH, 50mM Tris-HCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K	分辨率 3.19 Å R-free 0.279
4G0H Crystal structure of the N-terminal domain of Helicobacter pylori CagA protein 提交 2012-07-09	构建体不同突变/修饰不同聚集状态不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–884（884 aa）片段：UNP residues 1 to 884	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20% Ethanol, Na Cacodylate 100mM pH7, NaI 15mM, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 3.60 Å R-free 0.341

共 4 个其他 PDB 条目、7 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	CAGA_HELPY
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 5–221； UniProt 19–235 作者链 B； PDB构建体 5–221； UniProt 19–235 作者链 C； PDB构建体 5–221； UniProt 19–235 作者链 D； PDB构建体 5–221； UniProt 19–235

Apoptosis-stimulating of p53 protein 2

Homo sapiens

UniProt Q13625

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白异源复合物异源复合物蛋白 × 2 PDB 声明：dimeric（2）与蛋白拷贝数一致	链 E； UniProt 726–782	非标准单体：是（mmCIF未提供具体位点）	Cytotoxicity-associated immunodominant antigen × 1 （P55980）	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 8.5；Crystals were grown by vapor diffusion at 25 degrees C using hanging drops formed from mixing a 2ul of the protein complex with 2ul of an equilibration buffer (21% polyethylene glycol (PEG) molecular weight 4 kDa, 200 mM Li2 SO4 , and 100 mM Tris pH 8.5) and 0.6ul of the Silver Bullets additive 43 (Hampton Research HR2-996-43). Significantly higher quality crystals were obtained from selenomethionine-substituted protein complexes and they were used for the final refinement, VAPOR DIFFUSION, HANGING DROP	分辨率 2.04 Å R-free 0.234
2	蛋白异源复合物异源复合物蛋白 × 2 PDB 声明：dimeric（2）与蛋白拷贝数一致	链 F； UniProt 726–782	非标准单体：是（mmCIF未提供具体位点）	Cytotoxicity-associated immunodominant antigen × 1 （P55980）	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 8.5；Crystals were grown by vapor diffusion at 25 degrees C using hanging drops formed from mixing a 2ul of the protein complex with 2ul of an equilibration buffer (21% polyethylene glycol (PEG) molecular weight 4 kDa, 200 mM Li2 SO4 , and 100 mM Tris pH 8.5) and 0.6ul of the Silver Bullets additive 43 (Hampton Research HR2-996-43). Significantly higher quality crystals were obtained from selenomethionine-substituted protein complexes and they were used for the final refinement, VAPOR DIFFUSION, HANGING DROP	分辨率 2.04 Å R-free 0.234
3	蛋白异源复合物异源复合物蛋白 × 2 PDB 声明：dimeric（2）与蛋白拷贝数一致	链 G； UniProt 726–782	非标准单体：是（mmCIF未提供具体位点）	Cytotoxicity-associated immunodominant antigen × 1 （P55980）	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 8.5；Crystals were grown by vapor diffusion at 25 degrees C using hanging drops formed from mixing a 2ul of the protein complex with 2ul of an equilibration buffer (21% polyethylene glycol (PEG) molecular weight 4 kDa, 200 mM Li2 SO4 , and 100 mM Tris pH 8.5) and 0.6ul of the Silver Bullets additive 43 (Hampton Research HR2-996-43). Significantly higher quality crystals were obtained from selenomethionine-substituted protein complexes and they were used for the final refinement, VAPOR DIFFUSION, HANGING DROP	分辨率 2.04 Å R-free 0.234
4	蛋白异源复合物异源复合物蛋白 × 2 PDB 声明：dimeric（2）与蛋白拷贝数一致	链 H； UniProt 726–782	非标准单体：是（mmCIF未提供具体位点）	Cytotoxicity-associated immunodominant antigen × 1 （P55980）	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 8.5；Crystals were grown by vapor diffusion at 25 degrees C using hanging drops formed from mixing a 2ul of the protein complex with 2ul of an equilibration buffer (21% polyethylene glycol (PEG) molecular weight 4 kDa, 200 mM Li2 SO4 , and 100 mM Tris pH 8.5) and 0.6ul of the Silver Bullets additive 43 (Hampton Research HR2-996-43). Significantly higher quality crystals were obtained from selenomethionine-substituted protein complexes and they were used for the final refinement, VAPOR DIFFUSION, HANGING DROP	分辨率 2.04 Å R-free 0.234

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly， “相对当前条目”直接指出证据层面的不同；没有差异标签表示当前已读取字段一致。

其他 PDB	相对当前条目 4IRV	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
1YCS P53-53BP2 COMPLEX 提交 1996-09-30	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 B 769–1003（235 aa）片段：RESIDUES 327 - 519	未记录	ZN ZINC ION × 1	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.20 Å R-free 0.286
2UWQ Solution structure of ASPP2 N-terminus 提交 2007-03-22	构建体不同突变/修饰不同聚集状态不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–83（83 aa）片段：RESIDUES 1-83	未记录	未记录非水小分子	SOLUTION NMR NMR测量条件 pH 7.2；298 K；离子强度（mmCIF原始值）213；压力 1.0 NMR测量条件 pH 7.2；298 K；离子强度（mmCIF原始值）213；压力 1.0 NMR样品组成 25MM PHOSPHATE, PH7.2, 150MM NACL, 5MM DTT, 5%D2O	分辨率未提供
4A63 Crystal structure of the p73-ASPP2 complex at 2.6A resolution 提交 2011-10-31	构建体不同突变/修饰不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 B 892–1128（237 aa）片段：ANKYRIN AND SH3 DOMAINS, RESIDUES 892-1128	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 1.00M NA/KPO4, 0.1M ACETATE PH 4.5	分辨率 2.27 Å R-free 0.244
4A63 Crystal structure of the p73-ASPP2 complex at 2.6A resolution 提交 2011-10-31	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 D 892–1128（237 aa）片段：ANKYRIN AND SH3 DOMAINS, RESIDUES 892-1128	未记录	ZN ZINC ION × 1 ACT ACETATE ION × 3	X-RAY DIFFRACTION X-ray结晶条件 1.00M NA/KPO4, 0.1M ACETATE PH 4.5	分辨率 2.27 Å R-free 0.244
4A63 Crystal structure of the p73-ASPP2 complex at 2.6A resolution 提交 2011-10-31	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 F 892–1128（237 aa）片段：ANKYRIN AND SH3 DOMAINS, RESIDUES 892-1128	未记录	ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray结晶条件 1.00M NA/KPO4, 0.1M ACETATE PH 4.5	分辨率 2.27 Å R-free 0.244
4A63 Crystal structure of the p73-ASPP2 complex at 2.6A resolution 提交 2011-10-31	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 4 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 H 892–1128（237 aa）片段：ANKYRIN AND SH3 DOMAINS, RESIDUES 892-1128	未记录	ZN ZINC ION × 1 ACT ACETATE ION × 1	X-RAY DIFFRACTION X-ray结晶条件 1.00M NA/KPO4, 0.1M ACETATE PH 4.5	分辨率 2.27 Å R-free 0.244
4A63 Crystal structure of the p73-ASPP2 complex at 2.6A resolution 提交 2011-10-31	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 5 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 J 892–1128（237 aa）片段：ANKYRIN AND SH3 DOMAINS, RESIDUES 892-1128	未记录	ZN ZINC ION × 1 ACT ACETATE ION × 2	X-RAY DIFFRACTION X-ray结晶条件 1.00M NA/KPO4, 0.1M ACETATE PH 4.5	分辨率 2.27 Å R-free 0.244
4A63 Crystal structure of the p73-ASPP2 complex at 2.6A resolution 提交 2011-10-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 7 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 L 892–1128（237 aa）片段：ANKYRIN AND SH3 DOMAINS, RESIDUES 892-1128	未记录	ACT ACETATE ION × 1	X-RAY DIFFRACTION X-ray结晶条件 1.00M NA/KPO4, 0.1M ACETATE PH 4.5	分辨率 2.27 Å R-free 0.244
6GHM Structure of PP1 alpha phosphatase bound to ASPP2 提交 2018-05-08	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 D 926–1134（209 aa）	未记录	EDO 1,2-ETHANEDIOL × 8 GOL GLYCEROL × 3 NHE 2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID × 3 MN MANGANESE (II) ION × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M TRIS pH 8.5 16 % PEG 8000	分辨率 2.15 Å R-free 0.214
6GHM Structure of PP1 alpha phosphatase bound to ASPP2 提交 2018-05-08	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 C 926–1134（209 aa）	未记录	EDO 1,2-ETHANEDIOL × 4 GOL GLYCEROL × 1 NHE 2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID × 3 MN MANGANESE (II) ION × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M TRIS pH 8.5 16 % PEG 8000	分辨率 2.15 Å R-free 0.214
6HKP Factor Inhibiting HIF (FIH) in complex with zinc, NOG and ASPP2 (970-992) 提交 2018-09-07	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 S 976–998（23 aa）	未记录	SO4 SULFATE ION × 12 GOL GLYCEROL × 10 OGA N-OXALYLGLYCINE × 2 ZN ZINC ION × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M Hepes pH = 7.5, 2.0 M ammonium sulfate, 5.5% PEG400	分辨率 1.90 Å R-free 0.195

共 5 个其他 PDB 条目、11 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	ASPP2_HUMAN
Isoform	—
PDB实体	2
链与序列区间	作者链 E； PDB构建体 6–62； UniProt 726–782 作者链 F； PDB构建体 6–62； UniProt 726–782 作者链 G； PDB构建体 6–62； UniProt 726–782 作者链 H； PDB构建体 6–62； UniProt 726–782

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	4irv
沉积日期 deposition_date	2013-01-15
结构标题 title	Structure of the Helicobacter pylori CagA Oncogene Bound to the Human Tumor Suppressor Apoptosis-stimulating Protein of p53-2
关键词 keywords	Virulence factor and tumor suppressor, PROTEIN BINDING; PROTEIN BINDING
实验方法 method	X-RAY DIFFRACTION

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3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	34.00 Å
回转半径 Rg (电子) rg_electron	33.52 Å
零角强度 I(0) i0	157650000.00
分子量 molecular_weight	99895.0 kDa
排除体积 excluded_volume	124560 Å³
包络体积 envelope_volume	157940 Å³
水化壳体积 shell_volume	39640 Å³
包络直径 envelope_diameter	114.2 Å
壳层 Rg shell_rg	39.79 Å
包络 Rg envelope_rg	33.06 Å
形状 Rg shape_rg	33.51 Å
总 Rg total_rg	34.03 Å
总原子数 total_atoms	7017
残基数 n_residues	849
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	108.1 Å
Rg (实空间) rg_real	33.99 Å
Rg 误差 (实空间) rg_real_error	0.87 Å
I(0) (实空间) i0_real	1.5770e+08
I(0) 误差 (实空间) i0_real_error	2.7720e+06
Rg (倒空间) rg_reciprocal	34.00 Å
I(0) (倒空间) i0_reciprocal	157700000.0000
解质量估计 total_estimate	0.8986
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	43.7 Å
偏度 Skewness skewness	0.277
峰度 Kurtosis kurtosis	-0.500
角度范围 angular_range	— – 0.2350 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	56540000.0000
实空间数据点数 n_real_points	48
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.947; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.974; Smooth: 0.865

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5. 晶体学与实验 5. Crystallography & Experiment

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6. 实体与聚合物信息 Entities & Polymers (3)

实体 1 Entity 1 polymer

描述 description	Cytotoxicity-associated immunodominant antigen
分子量 formula_weight	25480.0 kDa
来源方法 src_method	man
拷贝数 entity_count	4

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D
原子数 atom_count	7530

GPVDTAFDPQQFINNLQVAFIKVDNVVASFDPDQKPIVDKNDRDNRQAFDGISQLREEYSNKAIKNPTKKNQYFSDFIDKSNDLINKDNLIDVESSTKSFQKFGDQRYQIFTSWVSHQKDPSKINTRSIRNFMENIIQPPIPDDKEKAEFLKSAKQSFAGIIIGNQIRTDQKFMGVFDESLKERQEAEKNGGPTGGDWLDIFLSFIFNKKQSSDVKEAINQ

来源 Source

来源生物 organism	Helicobacter pylori
NCBI 分类编号 ncbi_taxonomy_id	85962
宿主生物 host_organism	Escherichia coli

实体 2 Entity 2 polymer

描述 description	Apoptosis-stimulating of p53 protein 2
分子量 formula_weight	6609.3 kDa
来源方法 src_method	man
拷贝数 entity_count	4

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	E,F,G,H

GPKLASNAPRPLKKRSSITEPEGPNGPNIQKLLYQRTTIAAMETISVPSYPSKSASVTASSE

来源 Source

来源生物 organism	Homo sapiens
通用名 common_name	human
NCBI 分类编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Escherichia coli

实体 3 Entity 3 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	513

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7. 折叠分类 (SCOP + CATH) 4 domains

CATH v4.4 (4 domains)

结构域编号 domain_id	4irvA00
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	357 — Tetracycline Repressor; domain 2
同源超家族 H-superfamily homologous superfamily	130 —

结构域编号 domain_id	4irvB00
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	357 — Tetracycline Repressor; domain 2
同源超家族 H-superfamily homologous superfamily	130 —

结构域编号 domain_id	4irvC00
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	357 — Tetracycline Repressor; domain 2
同源超家族 H-superfamily homologous superfamily	130 —

结构域编号 domain_id	4irvD00
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	357 — Tetracycline Repressor; domain 2
同源超家族 H-superfamily homologous superfamily	130 —

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8. 引用文献 (1)

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晶胞边长 a length_a	118.641 Å
晶胞边长 b length_b	120.239 Å
晶胞边长 c length_c	100.659 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	115.64 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	16

空间群 space_group	C 1 2 1
空间群编号 int_tables_number	5
晶系 crystal_system	—

分辨率 resolution	2.040 Å
最低分辨率 resolution_low	90.750 Å
R 因子 r_factor	0.1915
R_work r_work	0.1893
R_free r_free	0.2341

fit	/app/data/saxs_computation/ir/4irv/4irv.fit
pddf	/app/data/pr_computation/ir/4irv/4irv.pr.out
profile	/app/data/saxs_computation/ir/4irv/4irv.dat

Structure of the Helicobacter pylori CagA Oncogene Bound to the Human Tumor Suppressor Apoptosis-stimulating Protein of p53-2

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Cytotoxicity-associated immunodominant antigen

当前结构中的状态

数据库中的同蛋白其他状态

Apoptosis-stimulating of p53 protein 2

当前结构中的状态

数据库中的同蛋白其他状态

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

2. 结构基本信息 2. Structure Basics

3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

5. 晶体学与实验 5. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

6. 实体与聚合物信息 Entities & Polymers (3)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 polymer

聚合物 Polymer

来源 Source

实体 3 Entity 3 water

7. 折叠分类 (SCOP + CATH) 4 domains

CATH v4.4 (4 domains)

8. 引用文献 (1)

9. 文件与曲线 (10)

Files (7)

Curves (3)