RIBONUCLEOTIDE REDUCTASE R1 PROTEIN
Escherichia coli
当前结构中的状态
| Assembly | 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与共同组分 | 实验方法与环境 | 结构质量 |
|---|---|---|---|---|---|---|
| 1 | 蛋白异源复合物 异源复合物 蛋白 × 3 PDB 声明：trimeric（3） 与蛋白拷贝数一致 | 链 A； UniProt 1–761 | 突变：C292A | RIBONUCLEOTIDE REDUCTASE R2 PROTEIN × 2 （P69924） TTP THYMIDINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION X-ray结晶条件：pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS | 分辨率 3.20 Å R-free 0.308 |
| 10 | 蛋白异源复合物 异源复合物 蛋白 × 2 PDB 声明：dimeric（2） 与蛋白拷贝数一致 | 链 A； UniProt 1–761 | 突变：C292A | RIBONUCLEOTIDE REDUCTASE R2 PROTEIN × 1 （P69924） TTP THYMIDINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION X-ray结晶条件：pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS | 分辨率 3.20 Å R-free 0.308 |
| 2 | 蛋白异源复合物 异源复合物 蛋白 × 2 PDB 声明：dimeric（2） 与蛋白拷贝数一致 | 链 B； UniProt 1–761 | 突变：C292A | RIBONUCLEOTIDE REDUCTASE R2 PROTEIN × 1 （P69924） TTP THYMIDINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION X-ray结晶条件：pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS | 分辨率 3.20 Å R-free 0.308 |
| 3 | 蛋白异源复合物 异源复合物 蛋白 × 2 PDB 声明：dimeric（2） 与蛋白拷贝数一致 | 链 C； UniProt 1–761 | 突变：C292A | RIBONUCLEOTIDE REDUCTASE R2 PROTEIN × 1 （P69924） TTP THYMIDINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION X-ray结晶条件：pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS | 分辨率 3.20 Å R-free 0.308 |
| 4 | 蛋白异源复合物 异源复合物 蛋白 × 15 PDB 声明：pentadecameric（15） 与蛋白拷贝数一致 | 链 A； UniProt 1–761 链 B； UniProt 1–761 | 突变：C292A | RIBONUCLEOTIDE REDUCTASE R2 PROTEIN × 9 （P69924） TTP THYMIDINE-5'-TRIPHOSPHATE × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 | X-RAY DIFFRACTION X-ray结晶条件：pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS | 分辨率 3.20 Å R-free 0.308 |
| 5 | 蛋白异源复合物 异源复合物 蛋白 × 12 PDB 声明：dodecameric（12） 与蛋白拷贝数一致 | 链 C； UniProt 1–761 | 突变：C292A | RIBONUCLEOTIDE REDUCTASE R2 PROTEIN × 6 （P69924） TTP THYMIDINE-5'-TRIPHOSPHATE × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 | X-RAY DIFFRACTION X-ray结晶条件：pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS | 分辨率 3.20 Å R-free 0.308 |
| 6 | 蛋白异源复合物 异源复合物 蛋白 × 5 PDB 声明：pentameric（5） 与蛋白拷贝数一致 | 链 A； UniProt 1–761 链 B； UniProt 1–761 | 突变：C292A | RIBONUCLEOTIDE REDUCTASE R2 PROTEIN × 3 （P69924） TTP THYMIDINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | X-RAY DIFFRACTION X-ray结晶条件：pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS | 分辨率 3.20 Å R-free 0.308 |
| 7 | 蛋白异源复合物 异源复合物 蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白拷贝数一致 | 链 C； UniProt 1–761 | 突变：C292A | RIBONUCLEOTIDE REDUCTASE R2 PROTEIN × 2 （P69924） TTP THYMIDINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | X-RAY DIFFRACTION X-ray结晶条件：pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS | 分辨率 3.20 Å R-free 0.308 |
| 8 | 蛋白异源复合物 异源复合物 蛋白 × 12 PDB 声明：dodecameric（12） 与蛋白拷贝数一致 | 链 A； UniProt 1–761 链 B； UniProt 1–761 | 突变：C292A | RIBONUCLEOTIDE REDUCTASE R2 PROTEIN × 6 （P69924） TTP THYMIDINE-5'-TRIPHOSPHATE × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 | X-RAY DIFFRACTION X-ray结晶条件：pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS | 分辨率 3.20 Å R-free 0.308 |
| 9 | 蛋白异源复合物 异源复合物 蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白拷贝数一致 | 链 A； UniProt 1–761 链 B； UniProt 1–761 | 突变：C292A | RIBONUCLEOTIDE REDUCTASE R2 PROTEIN × 2 （P69924） TTP THYMIDINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | X-RAY DIFFRACTION X-ray结晶条件：pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS | 分辨率 3.20 Å R-free 0.308 |
数据库中的同蛋白其他状态
以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly， “相对当前条目”直接指出证据层面的不同；没有差异标签表示当前已读取字段一致。
| 其他 PDB | 相对当前条目 4R1R | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法与环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1QFN GLUTAREDOXIN-1-RIBONUCLEOTIDE REDUCTASE B1 MIXED DISULFIDE BOND 提交 1999-04-12 | 构建体不同 突变/修饰不同 配体/离子不同 实验方法不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 B
737–761（25 aa）
片段：apha chain, B1 SUBUNIT
|
突变：C754S | 未记录非水小分子 |
SOLUTION NMR
NMR测量条件
pH 6.5；293 K；压力 1
|
分辨率未提供 |
| 1R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN MUTANT Y730F WITH A REDUCED ACTIVE SITE FROM ESCHERICHIA COLI 提交 1997-07-15 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric |
链 A
1–761（761 aa）
|
突变：Y730F | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 2.90 Å R-free 0.245 |
| 1R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN MUTANT Y730F WITH A REDUCED ACTIVE SITE FROM ESCHERICHIA COLI 提交 1997-07-15 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 B
1–761（761 aa）
|
突变：Y730F | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 2.90 Å R-free 0.245 |
| 1R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN MUTANT Y730F WITH A REDUCED ACTIVE SITE FROM ESCHERICHIA COLI 提交 1997-07-15 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 C
1–761（761 aa）
|
突变：Y730F | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 2.90 Å R-free 0.245 |
| 1R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN MUTANT Y730F WITH A REDUCED ACTIVE SITE FROM ESCHERICHIA COLI 提交 1997-07-15 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 4 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
突变：Y730F 突变：Y730F | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 2.90 Å R-free 0.245 |
| 1R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN MUTANT Y730F WITH A REDUCED ACTIVE SITE FROM ESCHERICHIA COLI 提交 1997-07-15 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 5 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 C
1–761（761 aa）
|
突变：Y730F | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 2.90 Å R-free 0.245 |
| 1R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN MUTANT Y730F WITH A REDUCED ACTIVE SITE FROM ESCHERICHIA COLI 提交 1997-07-15 | 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 6 蛋白同源多聚体 同源多聚体；蛋白 × 6 PDB 声明：hexameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
突变：Y730F 突变：Y730F | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 2.90 Å R-free 0.245 |
| 1RLR STRUCTURE OF RIBONUCLEOTIDE REDUCTASE PROTEIN R1 提交 1994-08-12 | 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric |
链 A
1–761（761 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION mmCIF 未提供已读取条件 | 分辨率 2.50 Å |
| 2R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH DTTP OCCUPYING THE SPECIFICITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric |
链 A
1–761（761 aa）
|
未记录 | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION
|
分辨率 3.00 Å R-free 0.280 |
| 2R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH DTTP OCCUPYING THE SPECIFICITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 B
1–761（761 aa）
|
未记录 | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION
|
分辨率 3.00 Å R-free 0.280 |
| 2R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH DTTP OCCUPYING THE SPECIFICITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 C
1–761（761 aa）
|
未记录 | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION
|
分辨率 3.00 Å R-free 0.280 |
| 2R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH DTTP OCCUPYING THE SPECIFICITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 4 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
未记录 | TTP THYMIDINE-5'-TRIPHOSPHATE × 6 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION
|
分辨率 3.00 Å R-free 0.280 |
| 2R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH DTTP OCCUPYING THE SPECIFICITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 5 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 C
1–761（761 aa）
|
未记录 | TTP THYMIDINE-5'-TRIPHOSPHATE × 6 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION
|
分辨率 3.00 Å R-free 0.280 |
| 2R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH DTTP OCCUPYING THE SPECIFICITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 6 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
未记录 | TTP THYMIDINE-5'-TRIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION
|
分辨率 3.00 Å R-free 0.280 |
| 2R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH DTTP OCCUPYING THE SPECIFICITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 7 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric |
链 C
1–761（761 aa）
|
未记录 | TTP THYMIDINE-5'-TRIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION
|
分辨率 3.00 Å R-free 0.280 |
| 2R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH DTTP OCCUPYING THE SPECIFICITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 8 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 A
1–761（761 aa）
|
未记录 | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION
|
分辨率 3.00 Å R-free 0.280 |
| 2X0X Ribonucleotide reductase R1 subunit of E. coli to 2.3 A resolution 提交 2009-12-18 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 C
1–761（761 aa）
|
未记录 | SO4 SULFATE ION × 6 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE APP 1.5M, SODIUM CITRATE BUFFER PH APP 6.
|
分辨率 2.30 Å R-free 0.224 |
| 2X0X Ribonucleotide reductase R1 subunit of E. coli to 2.3 A resolution 提交 2009-12-18 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
未记录 | SO4 SULFATE ION × 6 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE APP 1.5M, SODIUM CITRATE BUFFER PH APP 6.
|
分辨率 2.30 Å R-free 0.224 |
| 2XAK Ribonucleotide reductase Y730NO2Y modified R1 subunit of E. coli 提交 2010-03-31 | 构建体不同 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 A
1–761（761 aa）
片段：RESIDUES 1-761
链 B
1–761（761 aa）
片段：RESIDUES 1-761
|
非标准单体：是（mmCIF未提供具体位点） 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.
|
分辨率 2.80 Å R-free 0.241 |
| 2XAK Ribonucleotide reductase Y730NO2Y modified R1 subunit of E. coli 提交 2010-03-31 | 构建体不同 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 C
1–761（761 aa）
片段：RESIDUES 1-761
|
非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.
|
分辨率 2.80 Å R-free 0.241 |
| 2XAP Ribonucleotide reductase Y731NO2Y modified R1 subunit of E. coli to 2. 1 A resolution 提交 2010-03-31 | 构建体不同 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 A
1–761（761 aa）
片段：RESIDUES 1-761
链 B
1–761（761 aa）
片段：RESIDUES 1-761
|
非标准单体：是（mmCIF未提供具体位点） 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.
|
分辨率 2.10 Å R-free 0.230 |
| 2XAP Ribonucleotide reductase Y731NO2Y modified R1 subunit of E. coli to 2. 1 A resolution 提交 2010-03-31 | 构建体不同 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 C
1–761（761 aa）
片段：RESIDUES 1-761
|
非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.
|
分辨率 2.10 Å R-free 0.230 |
| 2XAV Ribonucleotide reductase Y731NO2Y and Y730F modified R1 subunit of E. coli 提交 2010-03-31 | 构建体不同 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 A
1–761（761 aa）
片段：RESIDUES 1-761
链 B
1–761（761 aa）
片段：RESIDUES 1-761
|
突变：YES 非标准单体：是（mmCIF未提供具体位点） 突变：YES 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.
|
分辨率 2.80 Å R-free 0.247 |
| 2XAV Ribonucleotide reductase Y731NO2Y and Y730F modified R1 subunit of E. coli 提交 2010-03-31 | 构建体不同 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 C
1–761（761 aa）
片段：RESIDUES 1-761
|
突变：YES 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.
|
分辨率 2.80 Å R-free 0.247 |
| 2XAW Ribonucleotide reductase Y730NO2Y and Y731F modified R1 subunit of E. coli 提交 2010-03-31 | 构建体不同 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 C
1–761（761 aa）
片段：RESIDUES 1-761
|
突变：YES 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.
|
分辨率 3.10 Å R-free 0.234 |
| 2XAW Ribonucleotide reductase Y730NO2Y and Y731F modified R1 subunit of E. coli 提交 2010-03-31 | 构建体不同 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 A
1–761（761 aa）
片段：RESIDUES 1-761
链 B
1–761（761 aa）
片段：RESIDUES 1-761
|
突变：YES 非标准单体：是（mmCIF未提供具体位点） 突变：YES 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.
|
分辨率 3.10 Å R-free 0.234 |
| 2XAX Ribonucleotide reductase Y730NO2Y and Y731A modified R1 subunit of E. coli 提交 2010-04-01 | 构建体不同 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 A
1–761（761 aa）
片段：RESIDUES 1-761
链 B
1–761（761 aa）
片段：RESIDUES 1-761
|
突变：YES 非标准单体：是（mmCIF未提供具体位点） 突变：YES 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.
|
分辨率 2.75 Å R-free 0.261 |
| 2XAX Ribonucleotide reductase Y730NO2Y and Y731A modified R1 subunit of E. coli 提交 2010-04-01 | 构建体不同 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 C
1–761（761 aa）
片段：RESIDUES 1-761
|
突变：YES 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.
|
分辨率 2.75 Å R-free 0.261 |
| 2XAY Ribonucleotide reductase Y730NO2Y and C439A modified R1 subunit of E. coli 提交 2010-04-01 | 构建体不同 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 A
1–761（761 aa）
片段：RESIDUES 1-761
链 B
1–761（761 aa）
片段：RESIDUES 1-761
|
突变：YES 非标准单体：是（mmCIF未提供具体位点） 突变：YES 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.
|
分辨率 2.65 Å R-free 0.235 |
| 2XAY Ribonucleotide reductase Y730NO2Y and C439A modified R1 subunit of E. coli 提交 2010-04-01 | 构建体不同 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 C
1–761（761 aa）
片段：RESIDUES 1-761
|
突变：YES 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.
|
分辨率 2.65 Å R-free 0.235 |
| 2XAZ Ribonucleotide reductase Y730NO2Y and C439S modified R1 subunit of E. coli 提交 2010-04-01 | 构建体不同 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 A
1–761（761 aa）
片段：RESIDUES 1-761
链 B
1–761（761 aa）
片段：RESIDUES 1-761
|
突变：YES 非标准单体：是（mmCIF未提供具体位点） 突变：YES 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.
|
分辨率 2.60 Å R-free 0.246 |
| 2XAZ Ribonucleotide reductase Y730NO2Y and C439S modified R1 subunit of E. coli 提交 2010-04-01 | 构建体不同 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 C
1–761（761 aa）
片段：RESIDUES 1-761
|
突变：YES 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.
|
分辨率 2.60 Å R-free 0.246 |
| 2XO4 RIBONUCLEOTIDE REDUCTASE Y730NH2Y MODIFIED R1 SUBUNIT OF E. COLI 提交 2010-08-09 | 构建体不同 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 C
1–761（761 aa）
片段：RESIDUES 1-761
|
非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.
|
分辨率 2.50 Å R-free 0.233 |
| 2XO4 RIBONUCLEOTIDE REDUCTASE Y730NH2Y MODIFIED R1 SUBUNIT OF E. COLI 提交 2010-08-09 | 构建体不同 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 A
1–761（761 aa）
片段：RESIDUES 1-761
链 B
1–761（761 aa）
片段：RESIDUES 1-761
|
非标准单体：是（mmCIF未提供具体位点） 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.
|
分辨率 2.50 Å R-free 0.233 |
| 2XO5 RIBONUCLEOTIDE REDUCTASE Y731NH2Y MODIFIED R1 SUBUNIT OF E. COLI 提交 2010-08-09 | 构建体不同 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 C
1–761（761 aa）
片段：CATALYTIC SUBUNIT, RESIDUES 1-761
|
非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.
|
分辨率 2.70 Å R-free 0.228 |
| 2XO5 RIBONUCLEOTIDE REDUCTASE Y731NH2Y MODIFIED R1 SUBUNIT OF E. COLI 提交 2010-08-09 | 构建体不同 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 A
1–761（761 aa）
片段：CATALYTIC SUBUNIT, RESIDUES 1-761
链 B
1–761（761 aa）
片段：CATALYTIC SUBUNIT, RESIDUES 1-761
|
非标准单体：是（mmCIF未提供具体位点） 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.
|
分辨率 2.70 Å R-free 0.228 |
| 3R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH AMPPNP OCCUPYING THE ACTIVITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric |
链 A
1–761（761 aa）
|
未记录 | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM AMPPNP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION
|
分辨率 3.00 Å R-free 0.287 |
| 3R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH AMPPNP OCCUPYING THE ACTIVITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 B
1–761（761 aa）
|
未记录 | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM AMPPNP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION
|
分辨率 3.00 Å R-free 0.287 |
| 3R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH AMPPNP OCCUPYING THE ACTIVITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 C
1–761（761 aa）
|
未记录 | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM AMPPNP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION
|
分辨率 3.00 Å R-free 0.287 |
| 3R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH AMPPNP OCCUPYING THE ACTIVITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 4 蛋白异源复合物 异源复合物；蛋白 × 15 PDB 声明：pentadecameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
未记录 | ATP ADENOSINE-5'-TRIPHOSPHATE × 6 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM AMPPNP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION
|
分辨率 3.00 Å R-free 0.287 |
| 3R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH AMPPNP OCCUPYING THE ACTIVITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 5 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 C
1–761（761 aa）
|
未记录 | ATP ADENOSINE-5'-TRIPHOSPHATE × 6 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM AMPPNP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION
|
分辨率 3.00 Å R-free 0.287 |
| 3R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH AMPPNP OCCUPYING THE ACTIVITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 6 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
未记录 | ATP ADENOSINE-5'-TRIPHOSPHATE × 6 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM AMPPNP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION
|
分辨率 3.00 Å R-free 0.287 |
| 3R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH AMPPNP OCCUPYING THE ACTIVITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21 | 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 7 蛋白异源复合物 异源复合物；蛋白 × 9 PDB 声明：nonameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
未记录 | ATP ADENOSINE-5'-TRIPHOSPHATE × 6 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM AMPPNP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION
|
分辨率 3.00 Å R-free 0.287 |
| 3R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH AMPPNP OCCUPYING THE ACTIVITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21 | 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 8 蛋白同源多聚体 同源多聚体；蛋白 × 6 PDB 声明：hexameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
未记录 | ATP ADENOSINE-5'-TRIPHOSPHATE × 6 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM AMPPNP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION
|
分辨率 3.00 Å R-free 0.287 |
| 3R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH AMPPNP OCCUPYING THE ACTIVITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 9 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 A
1–761（761 aa）
|
未记录 | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM AMPPNP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION
|
分辨率 3.00 Å R-free 0.287 |
| 3UUS Crystal structure of the dATP inhibited E. coli class Ia ribonucleotide reductase complex 提交 2011-11-28 | 构建体不同 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
链 C
1–761（761 aa）
链 D
1–761（761 aa）
|
未记录 | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 8 FE FE (III) ION × 8 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；10.8% PEG 3350, 0.18M magnesium acetate, 0.09M MOPS pH 7.5, 0.01M iron (III) chloride, 4.5% glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 5.65 Å R-free 0.303 |
| 4ERM Crystal structure of the dATP inhibited E. coli class Ia ribonucleotide reductase complex at 4 Angstroms resolution 提交 2012-04-20 | 构建体不同 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
链 C
1–761（761 aa）
链 D
1–761（761 aa）
|
未记录 | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 8 DAT 2'-DEOXYADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 4 FEO MU-OXO-DIIRON × 4 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Precipitant conditions: 10% PEG 3350, 0.1 M MOPS pH 7.5, 0.2 M Magnesium Acetate, 0.025 M Magnesium Chloride, 0.006 M n-Nonyl-beta-D-maltopyranoside, and 5% glycerol. Mixed 1:1 with protein., VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 3.95 Å R-free 0.284 |
| 4ERP Crystal structure of a gemcitabine-diphosphate inhibited E. coli class Ia ribonucleotide reductase complex 提交 2012-04-20 | 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
未记录 | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 FEO MU-OXO-DIIRON × 4 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Precipitant solution: 25% PEG 3350, 0.1 M HEPES pH 7.5, 0.2 M ammonium acetate, 5% glycerol mixed 2:1 with protein and streak seeded from crystals grown under similar conditions. , VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 4.45 Å R-free 0.267 |
| 4ERP Crystal structure of a gemcitabine-diphosphate inhibited E. coli class Ia ribonucleotide reductase complex 提交 2012-04-20 | 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric |
链 C
1–761（761 aa）
链 D
1–761（761 aa）
|
未记录 | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 FEO MU-OXO-DIIRON × 4 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Precipitant solution: 25% PEG 3350, 0.1 M HEPES pH 7.5, 0.2 M ammonium acetate, 5% glycerol mixed 2:1 with protein and streak seeded from crystals grown under similar conditions. , VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 4.45 Å R-free 0.267 |
| 5CNS Crystal structure of the dATP inhibited E. coli class Ia ribonucleotide reductase complex bound to CDP and dATP at 2.97 Angstroms resolution 提交 2015-07-18 | 构建体不同 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
链 C
1–761（761 aa）
链 D
1–761（761 aa）
|
未记录 | CDP CYTIDINE-5'-DIPHOSPHATE × 4 DAT 2'-DEOXYADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 8 DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 4 FEO MU-OXO-DIIRON × 4 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；9.5% (w/v) PEG 3350, 100 mM MOPS, 250 mM Mg(CH3COO)2, 25 mM
394 MgCl2, 5% (v/v) glycerol
|
分辨率 2.98 Å R-free 0.214 |
| 5CNT Crystal structure of the dATP inhibited E. coli class Ia ribonucleotide reductase complex bound to UDP and dATP at 3.25 Angstroms resolution 提交 2015-07-18 | 构建体不同 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
链 C
1–761（761 aa）
链 D
1–761（761 aa）
|
未记录 | UDP URIDINE-5'-DIPHOSPHATE × 4 DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 12 MG MAGNESIUM ION × 8 FEO MU-OXO-DIIRON × 4 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；2% (w/v) PEG 3350, 100 mM MOPS pH 7.5, 300 mM Mg(CH3COO)2, 30 mM MgCl2, and 5% (v/v) glycerol
|
分辨率 3.25 Å R-free 0.221 |
| 5CNU Crystal structure of the dATP inhibited E. coli class Ia ribonucleotide reductase complex bound to ADP and dGTP at 3.40 Angstroms resolution 提交 2015-07-18 | 构建体不同 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
链 C
1–761（761 aa）
链 D
1–761（761 aa）
|
未记录 | DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 4 DAT 2'-DEOXYADENOSINE-5'-DIPHOSPHATE × 4 FEO MU-OXO-DIIRON × 4 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；2% (w/v) PEG 3350, 100 mM MOPS pH 7.5, 300 mM Mg(CH3COO)2, 30 mM MgCl2, and 5% (v/v) glycerol
|
分辨率 3.40 Å R-free 0.221 |
| 5CNV Crystal structure of the dATP inhibited E. coli class Ia ribonucleotide reductase complex bound to GDP and TTP at 3.20 Angstroms resolution 提交 2015-07-18 | 构建体不同 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
链 C
1–761（761 aa）
链 D
1–761（761 aa）
|
未记录 | GDP GUANOSINE-5'-DIPHOSPHATE × 4 DAT 2'-DEOXYADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 8 TTP THYMIDINE-5'-TRIPHOSPHATE × 8 FEO MU-OXO-DIIRON × 4 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；2% (w/v) PEG 3350, 100 mM MOPS pH 7.5, 300 mM Mg(CH3COO)2, 30 mM MgCl2, and 5% (v/v) glycerol
|
分辨率 3.20 Å R-free 0.219 |
| 5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric |
链 A
1–761（761 aa）
|
突变：E441A | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.227 |
| 5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 10 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
突变：E441A 突变：E441A | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.227 |
| 5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 11 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric |
链 C
1–761（761 aa）
|
突变：E441A | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.227 |
| 5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 12 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 A
1–761（761 aa）
|
突变：E441A | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.227 |
| 5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 B
1–761（761 aa）
|
突变：E441A | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.227 |
| 5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 C
1–761（761 aa）
|
突变：E441A | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.227 |
| 5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 4 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
突变：E441A 突变：E441A | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.227 |
| 5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 5 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 C
1–761（761 aa）
|
突变：E441A | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.227 |
| 5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 6 蛋白异源复合物 异源复合物；蛋白 × 6 PDB 声明：hexameric |
链 B
1–761（761 aa）
|
突变：E441A | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.227 |
| 5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 7 蛋白异源复合物 异源复合物；蛋白 × 6 PDB 声明：hexameric |
链 A
1–761（761 aa）
|
突变：E441A | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.227 |
| 5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 8 蛋白异源复合物 异源复合物；蛋白 × 6 PDB 声明：hexameric |
链 C
1–761（761 aa）
|
突变：E441A | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.227 |
| 5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 9 蛋白同源多聚体 同源多聚体；蛋白 × 6 PDB 声明：hexameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
突变：E441A 突变：E441A | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.227 |
| 6R1R RIBONUCLEOTIDE REDUCTASE E441D MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric |
链 A
1–761（761 aa）
|
突变：E441D | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.240 |
| 6R1R RIBONUCLEOTIDE REDUCTASE E441D MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 B
1–761（761 aa）
|
突变：E441D | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.240 |
| 6R1R RIBONUCLEOTIDE REDUCTASE E441D MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 C
1–761（761 aa）
|
突变：E441D | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.240 |
| 6R1R RIBONUCLEOTIDE REDUCTASE E441D MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 4 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
突变：E441D 突变：E441D | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.240 |
| 6R1R RIBONUCLEOTIDE REDUCTASE E441D MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 5 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 C
1–761（761 aa）
|
突变：E441D | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.240 |
| 6R1R RIBONUCLEOTIDE REDUCTASE E441D MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 6 蛋白同源多聚体 同源多聚体；蛋白 × 6 PDB 声明：hexameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
突变：E441D 突变：E441D | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.240 |
| 6R1R RIBONUCLEOTIDE REDUCTASE E441D MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 7 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
突变：E441D 突变：E441D | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.240 |
| 6R1R RIBONUCLEOTIDE REDUCTASE E441D MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 8 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric |
链 C
1–761（761 aa）
|
突变：E441D | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.240 |
| 6R1R RIBONUCLEOTIDE REDUCTASE E441D MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 9 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 A
1–761（761 aa）
|
突变：E441D | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.240 |
| 6W4X Holocomplex of E. coli class Ia ribonucleotide reductase with GDP and TTP 提交 2020-03-11 | 突变/修饰不同 配体/离子不同 实验方法不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
未记录 | TTP THYMIDINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 FEO MU-OXO-DIIRON × 2 |
ELECTRON MICROSCOPY
cryo-EM缓冲液
pH 7.6
cryo-EM玻璃化条件
冷冻剂 ETHANE；blot for 4.5 seconds before plunging
|
分辨率 3.60 Å |
| 7R1R RIBONUCLEOTIDE REDUCTASE E441Q MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric |
链 A
1–761（761 aa）
|
突变：E441Q | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.231 |
| 7R1R RIBONUCLEOTIDE REDUCTASE E441Q MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 B
1–761（761 aa）
|
突变：E441Q | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.231 |
| 7R1R RIBONUCLEOTIDE REDUCTASE E441Q MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 C
1–761（761 aa）
|
突变：E441Q | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.231 |
| 7R1R RIBONUCLEOTIDE REDUCTASE E441Q MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 4 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 C
1–761（761 aa）
|
突变：E441Q | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.231 |
| 7R1R RIBONUCLEOTIDE REDUCTASE E441Q MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 5 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
突变：E441Q 突变：E441Q | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.231 |
| 7R1R RIBONUCLEOTIDE REDUCTASE E441Q MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 6 蛋白同源多聚体 同源多聚体；蛋白 × 6 PDB 声明：hexameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
突变：E441Q 突变：E441Q | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.231 |
| 7R1R RIBONUCLEOTIDE REDUCTASE E441Q MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 7 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric |
链 C
1–761（761 aa）
|
突变：E441Q | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.231 |
| 7R1R RIBONUCLEOTIDE REDUCTASE E441Q MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17 | 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 8 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
突变：E441Q 突变：E441Q | 未记录非水小分子 |
X-RAY DIFFRACTION
X-ray结晶条件
pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION
|
分辨率 3.10 Å R-free 0.231 |
| 8VHN Crystal Structure of E. coli class Ia ribonucleotide reductase alpha subunit bound to two ATP molecules 提交 2024-01-02 | 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
未记录 | ATP ADENOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 4 GOL GLYCEROL × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；293.15 K；8.0% (w/vol)PEG3350, 80 mM HEPES pH 7.3, 280 mM magnesium chloride, 4% (vol/vol) glycerol, 1.0% CYMAL-1 detergent
|
分辨率 2.62 Å R-free 0.209 |
| 8VHO Crystal Structure of E. coli class Ia ribonucleotide reductase alpha subunit bound to dATP 提交 2024-01-02 | 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
未记录 | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；277.15 K；6.8% (w/vol) PEG3350, 80mM HEPES pH7.3, 280mM MgCl2, 4% (vol/vol) glycerol, and 1.0% CYMAL-1 detergent
|
分辨率 2.55 Å R-free 0.205 |
| 8VHP Crystal structure of E. coli class Ia ribonucleotide reductase alpha subunit W28A variant bound to CDP and two molecules of ATP 提交 2024-01-02 | 构建体不同 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric |
链 A
1–760（760 aa）
链 B
1–760（760 aa）
|
突变：W28A 突变：W28A | SO4 SULFATE ION × 12 ATP ADENOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 4 CDP CYTIDINE-5'-DIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；291.15 K；1.9 M ammonium sulfate, 4% (w/vol) PEG MME 500, 0.1 M bis-Tris pH 6.5
|
分辨率 2.61 Å R-free 0.209 |
| 8VHP Crystal structure of E. coli class Ia ribonucleotide reductase alpha subunit W28A variant bound to CDP and two molecules of ATP 提交 2024-01-02 | 构建体不同 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric |
链 C
1–760（760 aa）
链 D
1–760（760 aa）
|
突变：W28A 突变：W28A | SO4 SULFATE ION × 12 ATP ADENOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 4 CDP CYTIDINE-5'-DIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；291.15 K；1.9 M ammonium sulfate, 4% (w/vol) PEG MME 500, 0.1 M bis-Tris pH 6.5
|
分辨率 2.61 Å R-free 0.209 |
| 8VHP Crystal structure of E. coli class Ia ribonucleotide reductase alpha subunit W28A variant bound to CDP and two molecules of ATP 提交 2024-01-02 | 构建体不同 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 3 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric |
链 E
1–760（760 aa）
链 F
1–760（760 aa）
|
突变：W28A 突变：W28A | SO4 SULFATE ION × 8 ATP ADENOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 4 CDP CYTIDINE-5'-DIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；291.15 K；1.9 M ammonium sulfate, 4% (w/vol) PEG MME 500, 0.1 M bis-Tris pH 6.5
|
分辨率 2.61 Å R-free 0.209 |
| 8VHP Crystal structure of E. coli class Ia ribonucleotide reductase alpha subunit W28A variant bound to CDP and two molecules of ATP 提交 2024-01-02 | 构建体不同 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 4 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric |
链 G
1–760（760 aa）
链 H
1–760（760 aa）
|
突变：W28A 突变：W28A | SO4 SULFATE ION × 8 ATP ADENOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 4 CDP CYTIDINE-5'-DIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；291.15 K；1.9 M ammonium sulfate, 4% (w/vol) PEG MME 500, 0.1 M bis-Tris pH 6.5
|
分辨率 2.61 Å R-free 0.209 |
| 8VHQ Crystal structure of E. coli class Ia ribonucleotide reductase alpha subunit W28A variant bound to dATP and ATP 提交 2024-01-02 | 构建体不同 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric |
链 A
1–760（760 aa）
链 B
1–760（760 aa）
|
突变：W28A 突变：W28A | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；277.15 K；6.5-7.0% (w/vol) PEG3350, 0.1M HEPES 7.0, 0.35M MgSO4, 5% (vol/vol) glycerol.
|
分辨率 3.40 Å R-free 0.233 |
| 8VHQ Crystal structure of E. coli class Ia ribonucleotide reductase alpha subunit W28A variant bound to dATP and ATP 提交 2024-01-02 | 构建体不同 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric |
链 C
1–760（760 aa）
链 D
1–760（760 aa）
|
突变：W28A 突变：W28A | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；277.15 K；6.5-7.0% (w/vol) PEG3350, 0.1M HEPES 7.0, 0.35M MgSO4, 5% (vol/vol) glycerol.
|
分辨率 3.40 Å R-free 0.233 |
| 8VHR Crystal structure of E. coli class Ia ribonucleotide reductase alpha subunit W28A variant bound to dATP and GTP 提交 2024-01-02 | 构建体不同 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric |
链 A
1–760（760 aa）
链 B
1–760（760 aa）
|
突变：W28A 突变：W28A | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；277.15 K；6.5-7.0% (w/vol) PEG3350, 0.1M HEPES 7.0, 0.35M MgSO4, 5% (vol/vol) glycerol
|
分辨率 3.55 Å R-free 0.243 |
| 8VHR Crystal structure of E. coli class Ia ribonucleotide reductase alpha subunit W28A variant bound to dATP and GTP 提交 2024-01-02 | 构建体不同 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric |
链 C
1–760（760 aa）
链 D
1–760（760 aa）
|
突变：W28A 突变：W28A | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；277.15 K；6.5-7.0% (w/vol) PEG3350, 0.1M HEPES 7.0, 0.35M MgSO4, 5% (vol/vol) glycerol
|
分辨率 3.55 Å R-free 0.243 |
| 8VHU Crystal structure of dATP bound E. coli class Ia ribonucleotide reductase alpha construct fused with the C-terminal tail of E. coli class Ia beta subunit 提交 2024-01-02 | 构建体不同 突变/修饰不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 信息不足 同源多聚体；蛋白 × 2 PDB 声明：dimeric |
链 A
1–734（734 aa）
链 B
1–734（734 aa）
|
突变：Truncated final 8 residues of alpha,fused C-terminal 35 residues of beta 突变：Truncated final 8 residues of alpha,fused C-terminal 35 residues of beta | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 3 NA SODIUM ION × 1 CL CHLORIDE ION × 6 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；277.15 K；1.4M NaCl, 8% (w/vol) PEG 6000
|
分辨率 2.10 Å R-free 0.192 |
| 9DB2 Class Ia ribonucleotide reductase with mechanism-based inhibitor N3CDP 提交 2024-08-23 | 突变/修饰不同 配体/离子不同 实验方法不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric |
链 A
1–761（761 aa）
链 B
1–761（761 aa）
|
未记录 | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 UNL UNKNOWN LIGAND × 1 A1A3L 4-amino-1-{(3xi)-3-C-amino-2-deoxy-5-O-[(S)-hydroxy(phosphonooxy)phosphoryl]-beta-D-threo-pentofuranosyl}pyrimidin-2(1H)-one × 1 FEO MU-OXO-DIIRON × 2 |
ELECTRON MICROSCOPY
cryo-EM缓冲液
pH 7.6
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.60 Å |
共 33 个其他 PDB 条目、95 个 assembly。 打开独立比较页并筛选聚集状态
查看构建体与数据证据
| UniProt名称 | RIR1_ECOLI |
| Isoform | — |
| PDB实体 | 1 |
| 链与序列区间 | 作者链 A； PDB构建体 1–761； UniProt 1–761 作者链 B； PDB构建体 1–761； UniProt 1–761 作者链 C； PDB构建体 1–761； UniProt 1–761 |
