5jtg

Crystal structure of Thermotoga maritima mutant D89K/D253K

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Cobalt/magnesium transport protein CorA

Thermotoga maritima

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 5	MAGNESIUM ION × 4 water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CORA_THEMA
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 24–373； UniProt 2–351 Author chain B； PDBConstruct 24–373； UniProt 2–351 Author chain C； PDBConstruct 24–373； UniProt 2–351 Author chain D； PDBConstruct 24–373； UniProt 2–351 Author chain E； PDBConstruct 24–373； UniProt 2–351

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	5jtg
Deposition date deposition_date	2016-05-09
Structure title title	Crystal structure of Thermotoga maritima mutant D89K/D253K
Keywords keywords	Transport Protein; TRANSPORT PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

5jtg__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

5jtg__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

5jtg__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	39.56 Å
Rg (electron density)	39.37 Å
Total Rg	39.80 Å
Atom count	13339
Residues	1607
Excluded volume	241960 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`5jtg__assembly_1__model_1`	pentameric （5）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Description description	Cobalt/magnesium transport protein CorA
Molecular weight formula_weight	44063.9 kDa
Mutation mutation	D89K, D253K
Source method src_method	man
Copy count entity_count	5

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E
Atom count atom_count	13344

MGSSHHHHHHSSGRENLYFQGHVEEKRLSAKKGLPPGTLVYTGKYREDFEIEVMNYSIEEFREFKTTDVESVLPFRDSSTPTWINITGIHRTDVVQRVGEFFGIHPLVLEKILNVHQRPKVEFFENYVFIVLKMFTYDKNLHELESEQVSLILTKNCVLMFQEKIGDVFDPVRERIRYNRGIIRKKRADYLLYSLIDALVDDYFVLLEKIDDEIDVLEEEVLERPEKETVQRTHQLKRNLVELRKTIWPLREVLSSLYRDVPPLIEKETVPYFRKVYDHTIQIADTVETFRDIVSGLLDVYLSSVSNKTNEVMKVLTIIATIFMPLTFIAGIYGMNFEYMPELRWKWGYPVVLAVMGVIAVIMVVYFKKKKWL

Source Source

Source organism organism	Thermotoga maritima
NCBI taxonomy ID ncbi_taxonomy_id	243274
Host organism host_organism	Escherichia coli

Entity 2 Entity 2 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 3 Entity 3 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	5

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6. Fold Classification (SCOP + CATH) 20 domains

SCOP 2.08 (5 domains)

Domain ID domain_id	d5jtga1
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.328 — CorA soluble domain-like
Superfamily Superfamily superfamily	d.328.1 — CorA soluble domain-like
Family Family family	d.328.1.1 — CorA soluble domain-like

Domain ID domain_id	d5jtgb1
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.328 — CorA soluble domain-like
Superfamily Superfamily superfamily	d.328.1 — CorA soluble domain-like
Family Family family	d.328.1.1 — CorA soluble domain-like

Domain ID domain_id	d5jtgc1
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.328 — CorA soluble domain-like
Superfamily Superfamily superfamily	d.328.1 — CorA soluble domain-like
Family Family family	d.328.1.1 — CorA soluble domain-like

Domain ID domain_id	d5jtgd1
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.328 — CorA soluble domain-like
Superfamily Superfamily superfamily	d.328.1 — CorA soluble domain-like
Family Family family	d.328.1.1 — CorA soluble domain-like

Domain ID domain_id	d5jtge1
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.328 — CorA soluble domain-like
Superfamily Superfamily superfamily	d.328.1 — CorA soluble domain-like
Family Family family	d.328.1.1 — CorA soluble domain-like

CATH v4.4 (15 domains)

Domain ID domain_id	5jtgA01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	460 — Beta Polymerase; domain 2
Homologous superfamily homologous superfamily	20 — CorA soluble domain-like

Domain ID domain_id	5jtgA02
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homologous superfamily homologous superfamily	340 — Magnesium transport protein CorA, transmembrane region

Domain ID domain_id	5jtgA03
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homologous superfamily homologous superfamily	340 — Magnesium transport protein CorA, transmembrane region

Domain ID domain_id	5jtgB01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	460 — Beta Polymerase; domain 2
Homologous superfamily homologous superfamily	20 — CorA soluble domain-like

Domain ID domain_id	5jtgB02
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homologous superfamily homologous superfamily	340 — Magnesium transport protein CorA, transmembrane region

Domain ID domain_id	5jtgB03
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homologous superfamily homologous superfamily	340 — Magnesium transport protein CorA, transmembrane region

Domain ID domain_id	5jtgC01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	460 — Beta Polymerase; domain 2
Homologous superfamily homologous superfamily	20 — CorA soluble domain-like

Domain ID domain_id	5jtgC02
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homologous superfamily homologous superfamily	340 — Magnesium transport protein CorA, transmembrane region

Domain ID domain_id	5jtgC03
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homologous superfamily homologous superfamily	340 — Magnesium transport protein CorA, transmembrane region

Domain ID domain_id	5jtgD01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	460 — Beta Polymerase; domain 2
Homologous superfamily homologous superfamily	20 — CorA soluble domain-like

Domain ID domain_id	5jtgD02
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homologous superfamily homologous superfamily	340 — Magnesium transport protein CorA, transmembrane region

Domain ID domain_id	5jtgD03
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homologous superfamily homologous superfamily	340 — Magnesium transport protein CorA, transmembrane region

Domain ID domain_id	5jtgE01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	460 — Beta Polymerase; domain 2
Homologous superfamily homologous superfamily	20 — CorA soluble domain-like

Domain ID domain_id	5jtgE02
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homologous superfamily homologous superfamily	340 — Magnesium transport protein CorA, transmembrane region

Domain ID domain_id	5jtgE03
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homologous superfamily homologous superfamily	340 — Magnesium transport protein CorA, transmembrane region

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Unit-cell length a length_a	122.125 Å
Unit-cell length b length_b	104.488 Å
Unit-cell length c length_c	122.821 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	106.13 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	10

Space group space_group	P 1 21 1
Space-group number int_tables_number	4
Crystal system crystal_system	—

Resolution resolution	3.050 Å
Low-resolution limit resolution_low	47.770 Å
R factor r_factor	0.2477
R_work r_work	0.2455
R_free r_free	0.2906

Crystal structure of Thermotoga maritima mutant D89K/D253K

1. Protein Identity and Related Structures Protein Identity & Related Structures

Cobalt/magnesium transport protein CorA

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

5jtg__assembly_1__model_1

5jtg__assembly_1__model_1 | I(q)

5jtg__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 water

6. Fold Classification (SCOP + CATH) 20 domains

SCOP 2.08 (5 domains)

CATH v4.4 (15 domains)

7. Citations (1)

Crystal structure of Thermotoga maritima mutant D89K/D253K

1. Protein Identity and Related Structures Protein Identity & Related Structures

Cobalt/magnesium transport protein CorA

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

5jtg__assembly_1__model_1

5jtg__assembly_1__model_1 | I(q)

5jtg__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 water

6. Fold Classification (SCOP + CATH) 20 domains

SCOP 2.08 (5 domains)

CATH v4.4 (15 domains)

7. Citations (1)