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6iol

Cryo-EM structure of multidrug efflux pump MexAB-OprM (60 degree state)

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Multidrug resistance protein MexA

Pseudomonas aeruginosa

UniProt P52477

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 12 Outer membrane protein OprM × 3 （Q51487） Multidrug resistance protein MexB × 3 （P52002） Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers
1t5e 1vf7 2v4d

3 assemblies

Heteromeric or nucleic-acid complex
4dk1 4dk1 6iok 6iol

4 assemblies

View Construct and Data Evidence
UniProt name MEXA_PSEAE
Isoform —
PDB entities 1
Chains and sequence ranges Author chain I； PDBConstruct 4–362； UniProt 25–383 Author chain J； PDBConstruct 4–362； UniProt 25–383 Author chain K； PDBConstruct 4–362； UniProt 25–383 Author chain L； PDBConstruct 4–362； UniProt 25–383 Author chain M； PDBConstruct 4–362； UniProt 25–383 Author chain N； PDBConstruct 4–362； UniProt 25–383

Outer membrane protein OprM

Pseudomonas aeruginosa PAO1

UniProt Q51487

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 12 Multidrug resistance protein MexA × 6 （P52477） Multidrug resistance protein MexB × 3 （P52002） Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers
1wp1 1wp1 3d5k 4y1k 4y1k 6zre 6zre 7akz 7akz

9 assemblies

Heteromeric or nucleic-acid complex
6iok 6iol 6ta5 6ta6

4 assemblies

View Construct and Data Evidence
UniProt name OPRM_PSEAE
Isoform —
PDB entities 2
Chains and sequence ranges Author chain A； PDBConstruct 1–468； UniProt 18–485 Author chain B； PDBConstruct 1–468； UniProt 18–485 Author chain C； PDBConstruct 1–468； UniProt 18–485

Multidrug resistance protein MexB

Pseudomonas aeruginosa PAO1

UniProt P52002

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 12 Multidrug resistance protein MexA × 6 （P52477） Outer membrane protein OprM × 3 （Q51487） Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers
21fo 21fp 2v50 2v50 3w9i 3w9i 3w9j 3w9j 6iia 6iia

10 assemblies

Heteromeric or nucleic-acid complex
6iok 6iol

2 assemblies

View Construct and Data Evidence
UniProt name MEXB_PSEAE
Isoform —
PDB entities 3
Chains and sequence ranges Author chain E； PDBConstruct 1–1046； UniProt 1–1046 Author chain F； PDBConstruct 1–1046； UniProt 1–1046 Author chain G； PDBConstruct 1–1046； UniProt 1–1046

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id6iol
Deposition date deposition_date2018-10-30
Structure title titleCryo-EM structure of multidrug efflux pump MexAB-OprM (60 degree state)
Keywords keywordsMultidrug resistance, efflux, complex, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

6iol__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

6iol__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

6iol__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）84.93 Å
Rg (electron density)87.76 Å
Total Rg87.31 Å
Atom count49077
Residues6450
Excluded volume877710 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 6iol__assembly_1__model_1 dodecameric （12） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

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6. Fold Classification (SCOP + CATH) 15 domains

CATH v4.4 (15 domains)

Domain ID domain_id6iolA01
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1600 — Outer membrane efflux proteins (OEP)
Homologous superfamily homologous superfamily10 — Outer membrane efflux proteins (OEP)
Domain ID domain_id6iolA02
Class class2 — Mainly Beta
Architecture architecture20 — Single Sheet
Topology topology200 — Outer membrane efflux proteins (OEP)
Homologous superfamily homologous superfamily10 — Outer membrane efflux proteins (OEP)
Domain ID domain_id6iolB01
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1600 — Outer membrane efflux proteins (OEP)
Homologous superfamily homologous superfamily10 — Outer membrane efflux proteins (OEP)
Domain ID domain_id6iolB02
Class class2 — Mainly Beta
Architecture architecture20 — Single Sheet
Topology topology200 — Outer membrane efflux proteins (OEP)
Homologous superfamily homologous superfamily10 — Outer membrane efflux proteins (OEP)
Domain ID domain_id6iolC01
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1600 — Outer membrane efflux proteins (OEP)
Homologous superfamily homologous superfamily10 — Outer membrane efflux proteins (OEP)
Domain ID domain_id6iolC02
Class class2 — Mainly Beta
Architecture architecture20 — Single Sheet
Topology topology200 — Outer membrane efflux proteins (OEP)
Homologous superfamily homologous superfamily10 — Outer membrane efflux proteins (OEP)
Domain ID domain_id6iolE01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology2090 — Multidrug efflux transporter AcrB TolC docking domain; DN and DC subdomains
Homologous superfamily homologous superfamily10 — Multidrug efflux transporter AcrB TolC docking domain; DN and DC subdomains
Domain ID domain_id6iolE02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology2090 — Multidrug efflux transporter AcrB TolC docking domain; DN and DC subdomains
Homologous superfamily homologous superfamily10 — Multidrug efflux transporter AcrB TolC docking domain; DN and DC subdomains
Domain ID domain_id6iolF01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology2090 — Multidrug efflux transporter AcrB TolC docking domain; DN and DC subdomains
Homologous superfamily homologous superfamily10 — Multidrug efflux transporter AcrB TolC docking domain; DN and DC subdomains
Domain ID domain_id6iolF02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology2090 — Multidrug efflux transporter AcrB TolC docking domain; DN and DC subdomains
Homologous superfamily homologous superfamily10 — Multidrug efflux transporter AcrB TolC docking domain; DN and DC subdomains
Domain ID domain_id6iolG01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology2090 — Multidrug efflux transporter AcrB TolC docking domain; DN and DC subdomains
Homologous superfamily homologous superfamily10 — Multidrug efflux transporter AcrB TolC docking domain; DN and DC subdomains
Domain ID domain_id6iolG02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology2090 — Multidrug efflux transporter AcrB TolC docking domain; DN and DC subdomains
Homologous superfamily homologous superfamily10 — Multidrug efflux transporter AcrB TolC docking domain; DN and DC subdomains
Domain ID domain_id6iolJ01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology287 — Helix Hairpins
Homologous superfamily homologous superfamily470 — Helix hairpin bin
Domain ID domain_id6iolK01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology287 — Helix Hairpins
Homologous superfamily homologous superfamily470 — Helix hairpin bin
Domain ID domain_id6iolM01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology287 — Helix Hairpins
Homologous superfamily homologous superfamily470 — Helix hairpin bin
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7. Citations (1)