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7dr7

bovine 20S immunoproteasome

Method: ELECTRON MICROSCOPY Dmax: 193.7 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Proteasome subunit alpha type-7

OrganismNot specified

UniProt Q3ZBG0

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain A； UniProt 1–248 Chain O； UniProt 1–248 Not recorded Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.30 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7DR7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain O 1–248（248 aa）
Chain a 1–248（248 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7DRW Bovine 20S immunoproteasome in complex with two human PA28alpha-beta activators Deposited 2020-12-29 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain D 1–248（248 aa）
Chain P 1–248（248 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–248（248 aa）
Chain R 1–248（248 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–248（248 aa）
Chain R 1–248（248 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 1–248（248 aa）
Chain R 1–248（248 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSA7_BOVIN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–248； UniProt 1–248 Author chain O； PDBConstruct 1–248； UniProt 1–248

Proteasome subunit alpha type-5

OrganismNot specified

UniProt Q5E987

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain B； UniProt 1–241 Chain P； UniProt 1–241 Not recorded Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.30 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7DR7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain P 1–241（241 aa）
Chain b 1–241（241 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7DRW Bovine 20S immunoproteasome in complex with two human PA28alpha-beta activators Deposited 2020-12-29 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain E 1–241（241 aa）
Chain R 1–241（241 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain E 1–241（241 aa）
Chain S 1–241（241 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain E 1–241（241 aa）
Chain S 1–241（241 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain E 1–241（241 aa）
Chain S 1–241（241 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSA5_BOVIN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–241； UniProt 1–241 Author chain P； PDBConstruct 1–241； UniProt 1–241

Proteasome subunit alpha type-1

OrganismNot specified

UniProt Q3T0X5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain C； UniProt 1–263 Chain Q； UniProt 1–263 Not recorded Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.30 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7DR7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain Q 1–263（263 aa）
Chain c 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7DRW Bovine 20S immunoproteasome in complex with two human PA28alpha-beta activators Deposited 2020-12-29 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain F 1–263（263 aa）
Chain b 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain F 1–263（263 aa）
Chain T 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain F 1–263（263 aa）
Chain T 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain F 1–263（263 aa）
Chain T 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSA1_BOVIN
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 1–263； UniProt 1–263 Author chain Q； PDBConstruct 1–263； UniProt 1–263

Proteasome subunit alpha type-3

OrganismNot specified

UniProt Q58DU5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain D； UniProt 1–255 Chain R； UniProt 1–255 Not recorded Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.30 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7DR7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain R 1–255（255 aa）
Chain d 1–255（255 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7DRW Bovine 20S immunoproteasome in complex with two human PA28alpha-beta activators Deposited 2020-12-29 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain G 1–255（255 aa）
Chain d 1–255（255 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain G 2–255（254 aa）
Chain U 2–255（254 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain G 1–255（255 aa）
Chain U 1–255（255 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain G 1–255（255 aa）
Chain U 1–255（255 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSA3_BOVIN
Isoform —
PDB entities 4
Chains and sequence ranges Author chain D； PDBConstruct 1–255； UniProt 1–255 Author chain R； PDBConstruct 1–255； UniProt 1–255

Proteasome subunit alpha type-6

OrganismNot specified

UniProt Q2YDE4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain E； UniProt 1–246 Chain L； UniProt 1–246 Not recorded Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.30 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7DR7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain L 1–246（246 aa）
Chain e 1–246（246 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7DRW Bovine 20S immunoproteasome in complex with two human PA28alpha-beta activators Deposited 2020-12-29 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain A 1–246（246 aa）
Chain f 1–246（246 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain A 1–246（246 aa）
Chain O 1–246（246 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain A 1–246（246 aa）
Chain O 1–246（246 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain A 1–246（246 aa）
Chain O 1–246（246 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSA6_BOVIN
Isoform —
PDB entities 5
Chains and sequence ranges Author chain E； PDBConstruct 1–246； UniProt 1–246 Author chain L； PDBConstruct 1–246； UniProt 1–246

Proteasome subunit alpha type-2

OrganismNot specified

UniProt Q3T0Y5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain F； UniProt 1–234 Chain M； UniProt 1–234 Not recorded Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.30 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7DR7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain M 1–234（234 aa）
Chain f 1–234（234 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7DRW Bovine 20S immunoproteasome in complex with two human PA28alpha-beta activators Deposited 2020-12-29 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain B 1–234（234 aa）
Chain h 1–234（234 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain B 2–234（233 aa）
Chain P 2–234（233 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain B 1–234（234 aa）
Chain P 1–234（234 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain B 1–234（234 aa）
Chain P 1–234（234 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSA2_BOVIN
Isoform —
PDB entities 6
Chains and sequence ranges Author chain F； PDBConstruct 1–234； UniProt 1–234 Author chain M； PDBConstruct 1–234； UniProt 1–234

Proteasome subunit alpha type-4

OrganismNot specified

UniProt Q3ZCK9

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain G； UniProt 1–261 Chain N； UniProt 1–261 Not recorded Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.30 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7DR7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain N 1–261（261 aa）
Chain g 1–261（261 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7DRW Bovine 20S immunoproteasome in complex with two human PA28alpha-beta activators Deposited 2020-12-29 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain C 1–261（261 aa）
Chain j 1–261（261 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain C 1–261（261 aa）
Chain Q 1–261（261 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain C 1–261（261 aa）
Chain Q 1–261（261 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain C 1–261（261 aa）
Chain Q 1–261（261 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSA4_BOVIN
Isoform —
PDB entities 7
Chains and sequence ranges Author chain G； PDBConstruct 1–261； UniProt 1–261 Author chain N； PDBConstruct 1–261； UniProt 1–261

Proteasome subunit beta type-3

OrganismNot specified

UniProt P33672

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain H； UniProt 1–205 Chain S； UniProt 1–205 Not recorded Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.30 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7DR7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1IRU Crystal Structure of the mammalian 20S proteasome at 2.75 A resolution Deposited 2001-10-24 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain J 1–192（192 aa）
Chain X 1–192（192 aa）
Not recorded MG MAGNESIUM ION × 30 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；MPD, Mg acetate, Na cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.75 Å R-free 0.294
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain H 1–205（205 aa）
Chain S 1–205（205 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7DRW Bovine 20S immunoproteasome in complex with two human PA28alpha-beta activators Deposited 2020-12-29 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain S 1–205（205 aa）
Chain k 1–205（205 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain J 1–205（205 aa）
Chain X 1–205（205 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain J 1–205（205 aa）
Chain X 1–205（205 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain J 1–205（205 aa）
Chain X 1–205（205 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.54 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSB3_BOVIN
Isoform —
PDB entities 8
Chains and sequence ranges Author chain H； PDBConstruct 1–205； UniProt 1–205 Author chain S； PDBConstruct 1–205； UniProt 1–205

Proteasome subunit beta type-2

OrganismNot specified

UniProt Q5E9K0

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain I； UniProt 1–201 Chain T； UniProt 1–201 Not recorded Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.30 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7DR7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain I 1–201（201 aa）
Chain T 1–201（201 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7DRW Bovine 20S immunoproteasome in complex with two human PA28alpha-beta activators Deposited 2020-12-29 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain T 1–201（201 aa）
Chain l 1–201（201 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain K 1–201（201 aa）
Chain Y 1–201（201 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain K 1–201（201 aa）
Chain Y 1–201（201 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain K 1–201（201 aa）
Chain Y 1–201（201 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSB2_BOVIN
Isoform —
PDB entities 9
Chains and sequence ranges Author chain I； PDBConstruct 1–201； UniProt 1–201 Author chain T； PDBConstruct 1–201； UniProt 1–201

Proteasome subunit beta type-1

OrganismNot specified

UniProt Q2TBX6

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain J； UniProt 1–241 Chain U； UniProt 1–241 Not recorded Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.30 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7DR7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain J 1–241（241 aa）
Chain U 1–241（241 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7DRW Bovine 20S immunoproteasome in complex with two human PA28alpha-beta activators Deposited 2020-12-29 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain U 1–241（241 aa）
Chain m 1–241（241 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 29–241（213 aa）
Chain M 29–241（213 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 1–241（241 aa）
Chain a 1–241（241 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain M 1–241（241 aa）
Chain a 1–241（241 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSB1_BOVIN
Isoform —
PDB entities 10
Chains and sequence ranges Author chain J； PDBConstruct 1–241； UniProt 1–241 Author chain U； PDBConstruct 1–241； UniProt 1–241

Proteasome subunit beta type-4

OrganismNot specified

UniProt Q3T108

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain K； UniProt 1–264 Chain V； UniProt 1–264 Not recorded Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.30 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7DR7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain K 1–264（264 aa）
Chain V 1–264（264 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7DRW Bovine 20S immunoproteasome in complex with two human PA28alpha-beta activators Deposited 2020-12-29 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain V 1–264（264 aa）
Chain n 1–264（264 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 2 46–264（219 aa）
Chain N 46–264（219 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain N 1–264（264 aa）
Chain b 1–264（264 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain N 1–264（264 aa）
Chain b 1–264（264 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSB4_BOVIN
Isoform —
PDB entities 11
Chains and sequence ranges Author chain K； PDBConstruct 1–264； UniProt 1–264 Author chain V； PDBConstruct 1–264； UniProt 1–264

Proteasome subunit beta type-9

OrganismNot specified

UniProt Q3SZC2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain W； UniProt 1–219 Chain Z； UniProt 1–219 Not recorded Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.30 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7DR7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain W 1–219（219 aa）
Chain Z 1–219（219 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7DRW Bovine 20S immunoproteasome in complex with two human PA28alpha-beta activators Deposited 2020-12-29 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain W 1–219（219 aa）
Chain Z 1–219（219 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSB9_BOVIN
Isoform —
PDB entities 12
Chains and sequence ranges Author chain W； PDBConstruct 1–219； UniProt 1–219 Author chain Z； PDBConstruct 1–219； UniProt 1–219

Proteasome subunit beta type-10

OrganismNot specified

UniProt Q3T0T1

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain 1； UniProt 1–273 Chain X； UniProt 1–273 Not recorded Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-8 × 2 （Q3T112） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.30 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7DR7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain 1 1–273（273 aa）
Chain X 1–273（273 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7DRW Bovine 20S immunoproteasome in complex with two human PA28alpha-beta activators Deposited 2020-12-29 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain 1 1–273（273 aa）
Chain X 1–273（273 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSB10_BOVIN
Isoform —
PDB entities 13
Chains and sequence ranges Author chain 1； PDBConstruct 1–273； UniProt 1–273 Author chain X； PDBConstruct 1–273； UniProt 1–273

Proteasome subunit beta type-8

OrganismNot specified

UniProt Q3T112

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain 2； UniProt 1–276 Chain Y； UniProt 1–276 Not recorded Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.30 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7DR7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain 2 1–276（276 aa）
Chain Y 1–276（276 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7DRW Bovine 20S immunoproteasome in complex with two human PA28alpha-beta activators Deposited 2020-12-29 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain 2 1–276（276 aa）
Chain Y 1–276（276 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSB8_BOVIN
Isoform —
PDB entities 14
Chains and sequence ranges Author chain 2； PDBConstruct 1–276； UniProt 1–276 Author chain Y； PDBConstruct 1–276； UniProt 1–276

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 7dr7

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 7dr7
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2. Structure Basics 2. Structure Basics

Entry ID entry_id7dr7
Deposition date deposition_date2020-12-26
Structure title titlebovine 20S immunoproteasome
Keywords keywordsproteasome, immunoproteasome, bovine spleen, HYDROLASE; HYDROLASE
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier60.03 Å
Radius of gyration Rg (electron density) rg_electron59.69 Å
Forward intensity I(0) i06628940000.00
Molecular weight molecular_weight688130.0 kDa
Excluded volume excluded_volume861580 ų
Envelope volume envelope_volume1271100 ų
Hydration-shell volume shell_volume169780 ų
Envelope diameter envelope_diameter193.9 Å
Shell Rg shell_rg68.09 Å
Envelope Rg envelope_rg57.94 Å
Shape Rg shape_rg59.68 Å
Total Rg total_rg59.89 Å
Total atoms total_atoms48304
Residues n_residues6220
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax193.7 Å
Rg (real space) rg_real59.75 Å
Rg uncertainty (real space) rg_real_error1.25 Å
I(0) (real space) i0_real6.6290e+09
I(0) uncertainty (real space) i0_real_error1.2890e+08
Rg (reciprocal space) rg_reciprocal60.25 Å
I(0) (reciprocal space) i0_reciprocal6634000000.0000
Solution quality estimate total_estimate0.7997
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary74.5 Å
Skewness Skewness skewness0.208
Kurtosis Kurtosis kurtosis-0.418
Angular range angular_range— – 0.1300 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha1103000000.0000
Real-space data points n_real_points27
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.812; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.955; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (14)

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7. Fold Classification (SCOP + CATH) 8 domains

CATH v4.4 (8 domains)

Domain ID domain_id7dr7A01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7dr7B01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7dr7F01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7dr7G01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7dr7M01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7dr7N01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7dr7O01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7dr7P01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
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8. Citations (1)

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9. Files and Curves (10)