7drw

Bovine 20S immunoproteasome in complex with two human PA28alpha-beta activators

Method: ELECTRON MICROSCOPY D_max: 230.3 Å Quality: GOOD

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Proteasome subunit alpha type-6

OrganismNot specified

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count	Chain A； UniProt 1–246 Chain f； UniProt 1–246	Not recorded	Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome activator complex subunit 2 × 6 （Q9UL46） Proteasome activator complex subunit 1 × 8 （Q06323） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 4.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 7DRW	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain L 1–246（246 aa） Chain e 1–246（246 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
7DR7 bovine 20S immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain E 1–246（246 aa） Chain L 1–246（246 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain A 1–246（246 aa） Chain O 1–246（246 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain A 1–246（246 aa） Chain O 1–246（246 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain A 1–246（246 aa） Chain O 1–246（246 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	PSA6_BOVIN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–246； UniProt 1–246 Author chain f； PDBConstruct 1–246； UniProt 1–246

Proteasome subunit alpha type-2

OrganismNot specified

UniProt Q3T0Y5

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count	Chain B； UniProt 1–234 Chain h； UniProt 1–234	Not recorded	Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome activator complex subunit 2 × 6 （Q9UL46） Proteasome activator complex subunit 1 × 8 （Q06323） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 4.20 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7DRW	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain M 1–234（234 aa） Chain f 1–234（234 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
7DR7 bovine 20S immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain F 1–234（234 aa） Chain M 1–234（234 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06	Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain B 2–234（233 aa） Chain P 2–234（233 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain B 1–234（234 aa） Chain P 1–234（234 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain B 1–234（234 aa） Chain P 1–234（234 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

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UniProt name	PSA2_BOVIN
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–234； UniProt 1–234 Author chain h； PDBConstruct 1–234； UniProt 1–234

Proteasome subunit alpha type-4

OrganismNot specified

UniProt Q3ZCK9

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count	Chain C； UniProt 1–261 Chain j； UniProt 1–261	Not recorded	Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome activator complex subunit 2 × 6 （Q9UL46） Proteasome activator complex subunit 1 × 8 （Q06323） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 4.20 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7DRW	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain N 1–261（261 aa） Chain g 1–261（261 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
7DR7 bovine 20S immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain G 1–261（261 aa） Chain N 1–261（261 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain C 1–261（261 aa） Chain Q 1–261（261 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain C 1–261（261 aa） Chain Q 1–261（261 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain C 1–261（261 aa） Chain Q 1–261（261 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	PSA4_BOVIN
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 1–261； UniProt 1–261 Author chain j； PDBConstruct 1–261； UniProt 1–261

Proteasome subunit alpha type-7

OrganismNot specified

UniProt Q3ZBG0

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count	Chain D； UniProt 1–248 Chain P； UniProt 1–248	Not recorded	Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome activator complex subunit 2 × 6 （Q9UL46） Proteasome activator complex subunit 1 × 8 （Q06323） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 4.20 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7DRW	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain O 1–248（248 aa） Chain a 1–248（248 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
7DR7 bovine 20S immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain A 1–248（248 aa） Chain O 1–248（248 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain D 1–248（248 aa） Chain R 1–248（248 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain D 1–248（248 aa） Chain R 1–248（248 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain D 1–248（248 aa） Chain R 1–248（248 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	PSA7_BOVIN
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain D； PDBConstruct 1–248； UniProt 1–248 Author chain P； PDBConstruct 1–248； UniProt 1–248

Proteasome subunit alpha type-5

OrganismNot specified

UniProt Q5E987

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count	Chain E； UniProt 1–241 Chain R； UniProt 1–241	Not recorded	Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome activator complex subunit 2 × 6 （Q9UL46） Proteasome activator complex subunit 1 × 8 （Q06323） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 4.20 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7DRW	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain P 1–241（241 aa） Chain b 1–241（241 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
7DR7 bovine 20S immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain B 1–241（241 aa） Chain P 1–241（241 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain E 1–241（241 aa） Chain S 1–241（241 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain E 1–241（241 aa） Chain S 1–241（241 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain E 1–241（241 aa） Chain S 1–241（241 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	PSA5_BOVIN
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain E； PDBConstruct 1–241； UniProt 1–241 Author chain R； PDBConstruct 1–241； UniProt 1–241

Proteasome subunit alpha type-1

OrganismNot specified

UniProt Q3T0X5

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count	Chain F； UniProt 1–263 Chain b； UniProt 1–263	Not recorded	Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome activator complex subunit 2 × 6 （Q9UL46） Proteasome activator complex subunit 1 × 8 （Q06323） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 4.20 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7DRW	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain Q 1–263（263 aa） Chain c 1–263（263 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
7DR7 bovine 20S immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain C 1–263（263 aa） Chain Q 1–263（263 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain F 1–263（263 aa） Chain T 1–263（263 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain F 1–263（263 aa） Chain T 1–263（263 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain F 1–263（263 aa） Chain T 1–263（263 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	PSA1_BOVIN
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain F； PDBConstruct 1–263； UniProt 1–263 Author chain b； PDBConstruct 1–263； UniProt 1–263

Proteasome subunit alpha type-3

OrganismNot specified

UniProt Q58DU5

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count	Chain G； UniProt 1–255 Chain d； UniProt 1–255	Not recorded	Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome activator complex subunit 2 × 6 （Q9UL46） Proteasome activator complex subunit 1 × 8 （Q06323） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 4.20 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7DRW	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain R 1–255（255 aa） Chain d 1–255（255 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
7DR7 bovine 20S immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain D 1–255（255 aa） Chain R 1–255（255 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06	Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain G 2–255（254 aa） Chain U 2–255（254 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain G 1–255（255 aa） Chain U 1–255（255 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain G 1–255（255 aa） Chain U 1–255（255 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	PSA3_BOVIN
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain G； PDBConstruct 1–255； UniProt 1–255 Author chain d； PDBConstruct 1–255； UniProt 1–255

Proteasome activator complex subunit 2

Homo sapiens

UniProt Q9UL46

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count	Chain H； UniProt 1–239 Chain K； UniProt 1–239 Chain M； UniProt 1–239 Chain O； UniProt 1–239 Chain c； UniProt 1–239 Chain g； UniProt 1–239	Not recorded	Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome activator complex subunit 1 × 8 （Q06323） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 4.20 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7DRW	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26	Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain A 1–239（239 aa） Chain D 1–239（239 aa） Chain F 1–239（239 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
7NAO Human PA28-20S proteasome complex Deposited 2021-06-22	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain d 1–239（239 aa） Chain f 1–239（239 aa） Chain h 1–239（239 aa） Chain i 1–239（239 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
7NAP Human PA28-20S-PA28 proteasome complex Deposited 2021-06-22	Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric	Chain d 1–239（239 aa） Chain f 1–239（239 aa） Chain h 1–239（239 aa） Chain i 1–239（239 aa） Chain k 1–239（239 aa） Chain m 1–239（239 aa） Chain o 1–239（239 aa） Chain p 1–239（239 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8CXB Human PA28-20S (PA28-4a3b) Deposited 2022-05-20	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain c 1–239（239 aa） Chain e 1–239（239 aa） Chain g 1–239（239 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	PSME2_HUMAN
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain H； PDBConstruct 1–239； UniProt 1–239 Author chain K； PDBConstruct 1–239； UniProt 1–239 Author chain M； PDBConstruct 1–239； UniProt 1–239 Author chain O； PDBConstruct 1–239； UniProt 1–239 Author chain c； PDBConstruct 1–239； UniProt 1–239 Author chain g； PDBConstruct 1–239； UniProt 1–239

Proteasome activator complex subunit 1

Homo sapiens

UniProt Q06323

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count	Chain I； UniProt 1–249 Chain J； UniProt 1–249 Chain L； UniProt 1–249 Chain N； UniProt 1–249 Chain Q； UniProt 1–249 Chain a； UniProt 1–249 Chain e； UniProt 1–249 Chain i； UniProt 1–249	Not recorded	Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome activator complex subunit 2 × 6 （Q9UL46） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 4.20 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7DRW	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
1AVO PROTEASOME ACTIVATOR REG(ALPHA) Deposited 1997-09-18	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 14 PDB declaration: tetradecameric	Chain A 4–63（60 aa） Chain B 104–242（139 aa） Chain C 4–63（60 aa） Chain D 104–242（139 aa） Chain E 4–63（60 aa） Chain F 104–242（139 aa） Chain G 4–63（60 aa） Chain H 104–242（139 aa） Chain I 4–63（60 aa） Chain J 104–242（139 aa） Chain K 4–63（60 aa） Chain L 104–242（139 aa） Chain M 4–63（60 aa） Chain N 104–242（139 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.1；pH 7.1	Resolution 2.80 Å R-free 0.289
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26	Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain B 1–249（249 aa） Chain C 1–249（249 aa） Chain E 1–249（249 aa） Chain G 1–249（249 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
7NAO Human PA28-20S proteasome complex Deposited 2021-06-22	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain c 1–249（249 aa） Chain e 1–249（249 aa） Chain g 1–249（249 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
7NAP Human PA28-20S-PA28 proteasome complex Deposited 2021-06-22	Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric	Chain c 1–249（249 aa） Chain e 1–249（249 aa） Chain g 1–249（249 aa） Chain j 1–249（249 aa） Chain l 1–249（249 aa） Chain n 1–249（249 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8CXB Human PA28-20S (PA28-4a3b) Deposited 2022-05-20	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain d 1–249（249 aa） Chain f 1–249（249 aa） Chain h 1–249（249 aa） Chain i 1–249（249 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	PSME1_HUMAN
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain I； PDBConstruct 1–249； UniProt 1–249 Author chain J； PDBConstruct 1–249； UniProt 1–249 Author chain L； PDBConstruct 1–249； UniProt 1–249 Author chain N； PDBConstruct 1–249； UniProt 1–249 Author chain Q； PDBConstruct 1–249； UniProt 1–249 Author chain a； PDBConstruct 1–249； UniProt 1–249 Author chain e； PDBConstruct 1–249； UniProt 1–249 Author chain i； PDBConstruct 1–249； UniProt 1–249

Proteasome subunit beta type-3

OrganismNot specified

UniProt P33672

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count	Chain S； UniProt 1–205 Chain k； UniProt 1–205	Not recorded	Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome activator complex subunit 2 × 6 （Q9UL46） Proteasome activator complex subunit 1 × 8 （Q06323） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 4.20 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7DRW	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
1IRU Crystal Structure of the mammalian 20S proteasome at 2.75 A resolution Deposited 2001-10-24	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain J 1–192（192 aa） Chain X 1–192（192 aa）	Not recorded	MG MAGNESIUM ION × 30	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；MPD, Mg acetate, Na cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.75 Å R-free 0.294
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain H 1–205（205 aa） Chain S 1–205（205 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
7DR7 bovine 20S immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain H 1–205（205 aa） Chain S 1–205（205 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain J 1–205（205 aa） Chain X 1–205（205 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain J 1–205（205 aa） Chain X 1–205（205 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain J 1–205（205 aa） Chain X 1–205（205 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.54 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	PSB3_BOVIN
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain S； PDBConstruct 1–205； UniProt 1–205 Author chain k； PDBConstruct 1–205； UniProt 1–205

Proteasome subunit beta type-2

OrganismNot specified

UniProt Q5E9K0

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count	Chain T； UniProt 1–201 Chain l； UniProt 1–201	Not recorded	Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome activator complex subunit 2 × 6 （Q9UL46） Proteasome activator complex subunit 1 × 8 （Q06323） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 4.20 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7DRW	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain I 1–201（201 aa） Chain T 1–201（201 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
7DR7 bovine 20S immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain I 1–201（201 aa） Chain T 1–201（201 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain K 1–201（201 aa） Chain Y 1–201（201 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain K 1–201（201 aa） Chain Y 1–201（201 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain K 1–201（201 aa） Chain Y 1–201（201 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	PSB2_BOVIN
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain T； PDBConstruct 1–201； UniProt 1–201 Author chain l； PDBConstruct 1–201； UniProt 1–201

Proteasome subunit beta type-1

OrganismNot specified

UniProt Q2TBX6

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count	Chain U； UniProt 1–241 Chain m； UniProt 1–241	Not recorded	Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome activator complex subunit 2 × 6 （Q9UL46） Proteasome activator complex subunit 1 × 8 （Q06323） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 4.20 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7DRW	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain J 1–241（241 aa） Chain U 1–241（241 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
7DR7 bovine 20S immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain J 1–241（241 aa） Chain U 1–241（241 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06	Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain 1 29–241（213 aa） Chain M 29–241（213 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain M 1–241（241 aa） Chain a 1–241（241 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain M 1–241（241 aa） Chain a 1–241（241 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	PSB1_BOVIN
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain U； PDBConstruct 1–241； UniProt 1–241 Author chain m； PDBConstruct 1–241； UniProt 1–241

Proteasome subunit beta type-4

OrganismNot specified

UniProt Q3T108

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count	Chain V； UniProt 1–264 Chain n； UniProt 1–264	Not recorded	Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome activator complex subunit 2 × 6 （Q9UL46） Proteasome activator complex subunit 1 × 8 （Q06323） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 4.20 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7DRW	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain K 1–264（264 aa） Chain V 1–264（264 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
7DR7 bovine 20S immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain K 1–264（264 aa） Chain V 1–264（264 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
8AZK Bovine 20S proteasome, untreated Deposited 2022-09-06	Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain 2 46–264（219 aa） Chain N 46–264（219 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8FZ5 The PI31-free Bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain N 1–264（264 aa） Chain b 1–264（264 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.23 Å
8FZ6 The human PI31 complexed with bovine 20S proteasome Deposited 2023-01-27	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain N 1–264（264 aa） Chain b 1–264（264 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20 mM Tris, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.54 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	PSB4_BOVIN
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain V； PDBConstruct 1–264； UniProt 1–264 Author chain n； PDBConstruct 1–264； UniProt 1–264

Proteasome subunit beta type-9

OrganismNot specified

UniProt Q3SZC2

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count	Chain W； UniProt 1–219 Chain Z； UniProt 1–219	Not recorded	Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome activator complex subunit 2 × 6 （Q9UL46） Proteasome activator complex subunit 1 × 8 （Q06323） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-10 × 2 （Q3T0T1） Proteasome subunit beta type-8 × 2 （Q3T112）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 4.20 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7DRW	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain W 1–219（219 aa） Chain Z 1–219（219 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
7DR7 bovine 20S immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain W 1–219（219 aa） Chain Z 1–219（219 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	PSB9_BOVIN
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain W； PDBConstruct 1–219； UniProt 1–219 Author chain Z； PDBConstruct 1–219； UniProt 1–219

Proteasome subunit beta type-10

OrganismNot specified

UniProt Q3T0T1

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count	Chain 1； UniProt 1–273 Chain X； UniProt 1–273	Not recorded	Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome activator complex subunit 2 × 6 （Q9UL46） Proteasome activator complex subunit 1 × 8 （Q06323） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-8 × 2 （Q3T112）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 4.20 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7DRW	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain 1 1–273（273 aa） Chain X 1–273（273 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
7DR7 bovine 20S immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain 1 1–273（273 aa） Chain X 1–273（273 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	PSB10_BOVIN
Isoform	—
PDB entities	15
Chains and sequence ranges	Author chain 1； PDBConstruct 1–273； UniProt 1–273 Author chain X； PDBConstruct 1–273； UniProt 1–273

Proteasome subunit beta type-8

OrganismNot specified

UniProt Q3T112

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count	Chain 2； UniProt 1–276 Chain Y； UniProt 1–276	Not recorded	Proteasome subunit alpha type-6 × 2 （Q2YDE4） Proteasome subunit alpha type-2 × 2 （Q3T0Y5） Proteasome subunit alpha type-4 × 2 （Q3ZCK9） Proteasome subunit alpha type-7 × 2 （Q3ZBG0） Proteasome subunit alpha type-5 × 2 （Q5E987） Proteasome subunit alpha type-1 × 2 （Q3T0X5） Proteasome subunit alpha type-3 × 2 （Q58DU5） Proteasome activator complex subunit 2 × 6 （Q9UL46） Proteasome activator complex subunit 1 × 8 （Q06323） Proteasome subunit beta type-3 × 2 （P33672） Proteasome subunit beta type-2 × 2 （Q5E9K0） Proteasome subunit beta type-1 × 2 （Q2TBX6） Proteasome subunit beta type-4 × 2 （Q3T108） Proteasome subunit beta type-9 × 2 （Q3SZC2） Proteasome subunit beta type-10 × 2 （Q3T0T1）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 4.20 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7DRW	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
7DR6 PA28alpha-beta in complex with immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric	Chain 2 1–276（276 aa） Chain Y 1–276（276 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
7DR7 bovine 20S immunoproteasome Deposited 2020-12-26	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain 2 1–276（276 aa） Chain Y 1–276（276 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	PSB8_BOVIN
Isoform	—
PDB entities	16
Chains and sequence ranges	Author chain 2； PDBConstruct 1–276； UniProt 1–276 Author chain Y； PDBConstruct 1–276； UniProt 1–276

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

Download Download SAXS (.dat) P(r) (.pr.out) CIF

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	7drw
Deposition date deposition_date	2020-12-29
Structure title title	Bovine 20S immunoproteasome in complex with two human PA28alpha-beta activators
Keywords keywords	proteasome, immunoproteasome, bovine spleen, PA28, HYDROLASE; HYDROLASE
Experimental Method method	ELECTRON MICROSCOPY

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	79.44 Å
Radius of gyration Rg (electron density) rg_electron	80.65 Å
Forward intensity I(0) i0	13978800000.00
Molecular weight molecular_weight	1019300.0 kDa
Excluded volume excluded_volume	1282300 Å³
Envelope volume envelope_volume	2002800 Å³
Hydration-shell volume shell_volume	214250 Å³
Envelope diameter envelope_diameter	309.5 Å
Shell Rg shell_rg	76.33 Å
Envelope Rg envelope_rg	79.22 Å
Shape Rg shape_rg	80.67 Å
Total Rg total_rg	80.52 Å
Total atoms total_atoms	71650
Residues n_residues	9108
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	230.3 Å
Rg (real space) rg_real	75.08 Å
Rg uncertainty (real space) rg_real_error	0.98 Å
I(0) (real space) i0_real	1.3410e+10
I(0) uncertainty (real space) i0_real_error	2.7020e+08
Rg (reciprocal space) rg_reciprocal	77.08 Å
I(0) (reciprocal space) i0_reciprocal	13890000000.0000
Solution quality estimate total_estimate	0.8927
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	2
Primary peak position r_peak_primary	80.6 Å
Skewness Skewness skewness	0.505
Kurtosis Kurtosis kurtosis	-0.246
Angular range angular_range	— – 0.1000 Å⁻¹
Current regularization parameter α current_alpha	0.5935
Highest regularization parameter α highest_alpha	3397000000.0000
Real-space data points n_real_points	21
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.001; Oscil: 0.880; Stabil: 0.980; Sysdev: 1.000; Positv: 1.000; Valcen: 0.971; Smooth: 0.061

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (16)

Entity 1 Entity 1 polymer

Description description	Proteasome subunit alpha type-6
Molecular weight formula_weight	27432.5 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,f
Atom count atom_count	71650

MSRGSSAGFDRHITIFSPEGRLYQVEYAFKAINQGGLTSVAVRGKDCAVIVTQKKVPDKLLDSSTVTHLFKITENIGCVMTGMTADSRSQVQRARYEAANWKYKYGYEIPVDMLCKRIADISQVYTQNAEMRPLGCCMILIGIDEEQGPQVYKCDPAGYYCGFKATAAGVKQTESTSFLEKKVKKKFDWTFEQTVETAITCLSTVLSIDFKPSEIEVGVVTVENPKFRILTEAEIDAHLVALAERD

Entity 2 Entity 2 polymer

Description description	Proteasome subunit alpha type-2
Molecular weight formula_weight	25927.5 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B,h

MAERGYSFSLTTFSPSGKLVQIEYALAAVAGGAPSVGIKAANGVVLATEKKQKSILYDERSVHKVEPITKHIGLVYSGMGPDYRVLVHRARKLAQQYYLVYQEPIPTAQLVQRVASVMQEYTQSGGVRPFGVSLLICGWNEGRPYLFQSDPSGAYFAWKATAMGKNYVNGKTFLEKRYNEDLELEDAIHTAILTLKESFEGQMTEDNIEVGICNEAGFRRLTPTEVKDYLAAIA

Entity 3 Entity 3 polymer

Description description	Proteasome subunit alpha type-4
Molecular weight formula_weight	29525.8 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C,j

MSRRYDSRTTIFSPEGRLYQVEYAMEAIGHAGTCLGILANDGVLLAAERRNIHKLLDEVFFSEKIYKLNEDMACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALCDIKQAYTQFGGKRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDYKEGEMTLKSALALAIKVLNKTMDVSKLSAEKVEIATLTRENGKTVIRVLKQKEVEQLIKKHEEEEAKAEREKKEKEQKEKDK

Entity 4 Entity 4 polymer

Description description	Proteasome subunit alpha type-7
Molecular weight formula_weight	27911.9 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D,P

MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGKDIVVLGVEKKSVAKLQDERTVRKICALDDNVCMAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRYIASLKQRYTQSNGRRPFGISALIVGFDFDGTPRLYQTDPSGTYHAWKANAIGRGAKSVREFLEKNYTDEAIETDDLTIKLVIKALLEVVQSGGKNIELAVMRRDQPLKILNPEEIEKYVAEIEKEKEENEKKKQKKAS

Entity 5 Entity 5 polymer

Description description	Proteasome subunit alpha type-5
Molecular weight formula_weight	26436.0 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E,R

MFLTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGIQTSEGVCLAVEKRITSPLMEPSSIEKIVEIDAHIGCAMSGLIADAKTLIDKARVETQNHWFTYNETMTVESVTQAVSNLALQFGEEDADPGAMSRPFGVALLFGGVDEKGPQLFHMDPSGTFVQCDARAIGSASEGAQSSLQEVYHKSMTLKEAIKSSLIILKQVMEEKLNATNIELATVQPGQNFHMFTKEELEEVIKDI

Entity 6 Entity 6 polymer

Description description	Proteasome subunit alpha type-1
Molecular weight formula_weight	29625.7 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F,b

MFRNQYDNDVTVWSPQGRIHQIEYAMEAVKQGSATVGLKSKTHAVLVALKRAQSELAAHQKKILHVDNHIGISIAGLTADARLLCNFMRQECLDSRFVFDRPLPVSRLVSLIGSKTQIPTQRYGRRPYGVGLLIAGYDDMGPHIFQTCPSANYFDCRAMSIGARSQSARTYLERHMSEFMECNLNELVKHGLRALRETLPAEQDLTTKNVSIGIVGKDLEFTIYDDDDVSPFLEGLEERPQRKAQPTQPADEPAEKADEPMEH

Entity 7 Entity 7 polymer

Description description	Proteasome subunit alpha type-3
Molecular weight formula_weight	28441.2 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G,d

MSSIGTGYDLSASTFSPDGRVFQVEYAMKAVENSSTAIGIRCKDGVVFGVEKLVLSKLYEEGSNKRLFNVDRHVGMAVAGLLADARSLADIAREEASNFRSNFGYNIPLKHLADRVAMYVHAYTLYSAVRPFGCSFMLGSYSVNDGAQLYMIDPSGVSYGYWGCAIGKARQAAKTEIEKLQMKEMTCRDVVKEVAKIIYIVHDEVKDKAFELELSWVGEITNGRHEIVPKDVREEAEKYAKESLKEEDESDDDNM

Entity 8 Entity 8 polymer

Description description	Proteasome activator complex subunit 2
Molecular weight formula_weight	27398.6 kDa
Source method src_method	man
Copy count entity_count	6

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H,K,M,O,c,g

MAKPCGVRLSGEARKQVEVFRQNLFQEAEEFLYRFLPQKIIYLNQLLQEDSLNVADLTSLRAPLDIPIPDPPPKDDEMETDKQEKKEVPKCGFLPGNEKVLSLLALVKPEVWTLKEKCILVITWIQHLIPKIEDGNDFGVAIQEKVLERVNAVKTKVEAFQTTISKYFSERGDAVAKASKETHVMDYRALVHERDEAAYGELRAMVLDLRAFYAELYHIISSNLEKIVNPKGEEKPSMY

来源 Source

来源生物 organism	Homo sapiens
通用名 common_name	Human
NCBI Class编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Escherichia coli

Entity 9 Entity 9 polymer

Description description	Proteasome activator complex subunit 1
Molecular weight formula_weight	28768.1 kDa
Source method src_method	man
Copy count entity_count	8

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I,J,L,N,Q,a,e,i

MAMLRVQPEAQAKVDVFREDLCTKTENLLGSYFPKKISELDAFLKEPALNEANLSNLKAPLDIPVPDPVKEKEKEERKKQQEKEDKDEKKKGEDEDKGPPCGPVNCNEKIVVLLQRLKPEIKDVIEQLNLVTTWLQLQIPRIEDGNNFGVAVQEKVFELMTSLHTKLEGFHTQISKYFSERGDAVTKAAKQPHVGDYRQLVHELDEAEYRDIRLMVMEIRNAYAVLYDIILKNFEKLKKPRGETKGMIY

来源 Source

来源生物 organism	Homo sapiens
通用名 common_name	Human
NCBI Class编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Escherichia coli

Entity 10 Entity 10 polymer

Description description	Proteasome subunit beta type-3
Molecular weight formula_weight	23016.9 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	k,S

MSIMSYNGGAVMAMKGKNCVAIAADRRFGIQAQMVTTDFQKIFPMGDRLYIGLAGLATDVQTVAQRLKFRLNLYELKEGRQIKPYTLMSMVANLLYEKRFGPYYTEPVIAGLDPKTFKPFICSLDLIGCPMVTDDFVVSGTCTEQMYGMCESLWEPNMDPEHLFETISQAMLNAVDRDAVSGMGVIVHIIEKDKITTRTLKARMD

Entity 11 Entity 11 polymer

Description description	Proteasome subunit beta type-2
Molecular weight formula_weight	22924.3 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	l,T

MEYLIGIQGPDYVLVASDRVAASNIVQMKDDHDKMFKMSEKILLLCVGEAGDTVQFAEYIQKNVQLYKMRNGYELSPTAAANFTRRNLADYLRSRTPYHVNLLLAGYDEHEGPALYYMDYLAALAKAPFAAHGYGAFLTLSILDRYYTPTISREKAVELLRKCLEELQKRFILNLPTFSVRIIDRNGIHDLDNISFPKQGS

Entity 12 Entity 12 polymer

Description description	Proteasome subunit beta type-1
Molecular weight formula_weight	26278.1 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	m,U

MLSSVAAYSGAGRDLAMEPHSSVGPLQLRFSPYAFNGGTVLAIAGEDFSIVASDTRLSEGFSIHTRDSPKCYKLTDKTVIGCSGFHGDCLTLTKIIEARLKMYKHSNNKAMTTGAIAAMLSTILYSRRFFPYYVYNIIGGLDEEGKGAVYSFDPVGSYQRDSFKAGGSASAMLQPLLDNQVGFKNMQNVEHVPLSLDRAMRLVKDVFISAAERDVYTGDALKVCIVTKEGIRGETVPLRKD

Entity 13 Entity 13 polymer

Description description	Proteasome subunit beta type-4
Molecular weight formula_weight	29057.0 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	n,V

MEALLESRSGLWAGGPAPGQFYRIPPTPGSSVDPVSALYGSPITRTQNPMVTGTSVLGLKFEGGVVIAADMLGSYGSLARFRNISRIMRVNNSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPKAIHSWLTRAMYSRRSKMNPLWNTMVIGGYADGESFLGYVDMLGVAYEAPSLATGYGAYLAQPLLREVLEKQPVLSQTEARELVERCMRVLYYRDARSYNRFQIATVTEKGVEIEGPLSAETNWDIAHMISGFE

Entity 14 Entity 14 polymer

Description description	Proteasome subunit beta type-9
Molecular weight formula_weight	23427.4 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	W,Z

MLRTGAPNGDLPRAGEVHTGTTIMAVEFDGGVVVGSDSRVSAGEAVVNRVFDKLSPLHQHIYCALSGSAADAQAIADMAAYQLELHGMELEEPPLVLAAANVVRNITYKYREDLSAHLMVAGWDQREGGQVYGTMSGMLIRQPFAIGGSGSTYIYGYVDAAYKPGMSPEECRRFTTNAIALAMKRDGSSGGVIYLATITGAGVDHRVILGDELPRFYDE

Entity 15 Entity 15 polymer

Description description	Proteasome subunit beta type-10
Molecular weight formula_weight	29128.3 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	X,1

MQKTVLEPQRGFSFENCERNAALQRALPGLRVPHARKTGTTIAGLVFQDGVILGADTRATNDSVVADKICEKIHFIAPKIYCCGAGVAADAEMTTRMAASNMELHALSTGRECRVATVTRMLRQTLFRYQGYVGASLIVGGVDFTGPQLYSVHPHGSYSRLPFTALGSGQDAAIAVLEDRFQPNMTLEAAQELLVEAITAGILGDLGSGGNVDACVITAAGAKMLRALSSPTKPIERSSQYRFAPGTTPVLSQTVVPLTLELVEETVQAMDVE

Entity 16 Entity 16 polymer

Description description	Proteasome subunit beta type-8
Molecular weight formula_weight	30304.3 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Y,2

MALLDVCGAPGGQRGDWAVPLAGSRQRSDPGHYGFSLRSPELALPRGMQPTEFFRSLGGNGESKVQIEMAHGTTTLAFKFQHGVIVAVDSRASAGNYIATLKVNKVIEINPYLLGTMSGCAADCLYWERLLAKECRLYYLRNGERISVSAASKLLSNMMCQYRGMGLSMGSMICGWDKKGPGLYYVDENGTRLSGNMFSTGSGNSHAYGVMDSGYRPDLSIEEAYDLGRRAIVHATHRDSYSGGVVNMYHMKEDGWVKVESTDVSDLMHQYREASQ

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8. Citations (1)

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Resolution resolution	4.20 Å
Resolution测定方法 resolution_method	—
颗粒数 num_particles	24657

fit	/app/data/saxs_computation/dr/7drw/7drw.fit
pddf	/app/data/pr_computation/dr/7drw/7drw.pr.out
profile	/app/data/saxs_computation/dr/7drw/7drw.dat

Bovine 20S immunoproteasome in complex with two human PA28alpha-beta activators

1. Protein Identity and Related Structures Protein Identity & Related Structures

Proteasome subunit alpha type-6

State in the Current Structure

Other States of the Same Protein in the Database

Proteasome subunit alpha type-2

State in the Current Structure

Other States of the Same Protein in the Database

Proteasome subunit alpha type-4

State in the Current Structure

Other States of the Same Protein in the Database

Proteasome subunit alpha type-7

State in the Current Structure

Other States of the Same Protein in the Database

Proteasome subunit alpha type-5

State in the Current Structure

Other States of the Same Protein in the Database

Proteasome subunit alpha type-1

State in the Current Structure

Other States of the Same Protein in the Database

Proteasome subunit alpha type-3

State in the Current Structure

Other States of the Same Protein in the Database

Proteasome activator complex subunit 2

State in the Current Structure

Other States of the Same Protein in the Database

Proteasome activator complex subunit 1

State in the Current Structure

Other States of the Same Protein in the Database

Proteasome subunit beta type-3

State in the Current Structure

Other States of the Same Protein in the Database

Proteasome subunit beta type-2

State in the Current Structure

Other States of the Same Protein in the Database

Proteasome subunit beta type-1

State in the Current Structure

Other States of the Same Protein in the Database

Proteasome subunit beta type-4

State in the Current Structure

Other States of the Same Protein in the Database

Proteasome subunit beta type-9

State in the Current Structure

Other States of the Same Protein in the Database

Proteasome subunit beta type-10

State in the Current Structure

Other States of the Same Protein in the Database

Proteasome subunit beta type-8

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

冷冻电镜 EM 3D

6. Entities and Polymers Entities & Polymers (16)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Entity 4 Entity 4 polymer

Polymer Polymer

Entity 5 Entity 5 polymer

Polymer Polymer

Entity 6 Entity 6 polymer

Polymer Polymer

Entity 7 Entity 7 polymer

Polymer Polymer

Entity 8 Entity 8 polymer

Polymer Polymer

来源 Source

Entity 9 Entity 9 polymer

Polymer Polymer

来源 Source

Entity 10 Entity 10 polymer

Polymer Polymer