7kin

Mycobacterium tuberculosis WT RNAP transcription open promoter complex with WhiB7 promoter

Method: ELECTRON MICROSCOPY
▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

DNA-directed RNA polymerase subunit alpha

Mycobacterium tuberculosis

UniProt A5U8D3

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 8 DNA 2 DNA-directed RNA polymerase subunit beta × 1 (A5U052) ;DNA-directed RNA polymerase subunit beta' ; × 1 (A0A045J9E2) DNA-directed RNA polymerase subunit omega × 1 (A0A0T9N9K3) RNA polymerase sigma factor SigA × 1 (A0A0H3LGM9) RNA polymerase-binding protein RbpA × 1 (P9WHJ4) RNA polymerase-binding transcription factor CarD × 1 (P9WJG2) DNA (54-MER) × 1 DNA (49-MER) × 1 ZINC ION × 2 MAGNESIUM ION × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name RPOA_MYCTA
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A; PDBConstruct 1–347; UniProt 1–347 Author chain B; PDBConstruct 1–347; UniProt 1–347

DNA-directed RNA polymerase subunit beta

Mycobacterium tuberculosis

UniProt A5U052

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 8 DNA 2 DNA-directed RNA polymerase subunit alpha × 2 (A5U8D3) ;DNA-directed RNA polymerase subunit beta' ; × 1 (A0A045J9E2) DNA-directed RNA polymerase subunit omega × 1 (A0A0T9N9K3) RNA polymerase sigma factor SigA × 1 (A0A0H3LGM9) RNA polymerase-binding protein RbpA × 1 (P9WHJ4) RNA polymerase-binding transcription factor CarD × 1 (P9WJG2) DNA (54-MER) × 1 DNA (49-MER) × 1 ZINC ION × 2 MAGNESIUM ION × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name RPOB_MYCTA
Isoform —
PDB entities 2
Chains and sequence ranges Author chain C; PDBConstruct 1–1172; UniProt 6–1177

;DNA-directed RNA polymerase subunit beta' ;

Mycobacterium tuberculosis

UniProt A0A045J9E2

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 8 DNA 2 DNA-directed RNA polymerase subunit alpha × 2 (A5U8D3) DNA-directed RNA polymerase subunit beta × 1 (A5U052) DNA-directed RNA polymerase subunit omega × 1 (A0A0T9N9K3) RNA polymerase sigma factor SigA × 1 (A0A0H3LGM9) RNA polymerase-binding protein RbpA × 1 (P9WHJ4) RNA polymerase-binding transcription factor CarD × 1 (P9WJG2) DNA (54-MER) × 1 DNA (49-MER) × 1 ZINC ION × 2 MAGNESIUM ION × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name A0A045J9E2_MYCTX
Isoform —
PDB entities 3
Chains and sequence ranges Author chain D; PDBConstruct 3–1318; UniProt 1–1316

DNA-directed RNA polymerase subunit omega

Mycobacterium tuberculosis

UniProt A0A0T9N9K3

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 8 DNA 2 DNA-directed RNA polymerase subunit alpha × 2 (A5U8D3) DNA-directed RNA polymerase subunit beta × 1 (A5U052) ;DNA-directed RNA polymerase subunit beta' ; × 1 (A0A045J9E2) RNA polymerase sigma factor SigA × 1 (A0A0H3LGM9) RNA polymerase-binding protein RbpA × 1 (P9WHJ4) RNA polymerase-binding transcription factor CarD × 1 (P9WJG2) DNA (54-MER) × 1 DNA (49-MER) × 1 ZINC ION × 2 MAGNESIUM ION × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name A0A0T9N9K3_MYCTX
Isoform —
PDB entities 4
Chains and sequence ranges Author chain E; PDBConstruct 1–110; UniProt 40–149

RNA polymerase sigma factor SigA

Mycobacterium tuberculosis

UniProt A0A0H3LGM9

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 8 DNA 2 DNA-directed RNA polymerase subunit alpha × 2 (A5U8D3) DNA-directed RNA polymerase subunit beta × 1 (A5U052) ;DNA-directed RNA polymerase subunit beta' ; × 1 (A0A045J9E2) DNA-directed RNA polymerase subunit omega × 1 (A0A0T9N9K3) RNA polymerase-binding protein RbpA × 1 (P9WHJ4) RNA polymerase-binding transcription factor CarD × 1 (P9WJG2) DNA (54-MER) × 1 DNA (49-MER) × 1 ZINC ION × 2 MAGNESIUM ION × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name A0A0H3LGM9_MYCTE
Isoform —
PDB entities 5
Chains and sequence ranges Author chain F; PDBConstruct 1–528; UniProt 3–530

RNA polymerase-binding protein RbpA

Mycobacterium tuberculosis

UniProt P9WHJ4

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 8 DNA 2 DNA-directed RNA polymerase subunit alpha × 2 (A5U8D3) DNA-directed RNA polymerase subunit beta × 1 (A5U052) ;DNA-directed RNA polymerase subunit beta' ; × 1 (A0A045J9E2) DNA-directed RNA polymerase subunit omega × 1 (A0A0T9N9K3) RNA polymerase sigma factor SigA × 1 (A0A0H3LGM9) RNA polymerase-binding transcription factor CarD × 1 (P9WJG2) DNA (54-MER) × 1 DNA (49-MER) × 1 ZINC ION × 2 MAGNESIUM ION × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name RBPA_MYCTO
Isoform —
PDB entities 6
Chains and sequence ranges Author chain J; PDBConstruct 1–111; UniProt 1–111

RNA polymerase-binding transcription factor CarD

Mycobacterium tuberculosis

UniProt P9WJG2

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 8 DNA 2 DNA-directed RNA polymerase subunit alpha × 2 (A5U8D3) DNA-directed RNA polymerase subunit beta × 1 (A5U052) ;DNA-directed RNA polymerase subunit beta' ; × 1 (A0A045J9E2) DNA-directed RNA polymerase subunit omega × 1 (A0A0T9N9K3) RNA polymerase sigma factor SigA × 1 (A0A0H3LGM9) RNA polymerase-binding protein RbpA × 1 (P9WHJ4) DNA (54-MER) × 1 DNA (49-MER) × 1 ZINC ION × 2 MAGNESIUM ION × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name CARD_MYCTO
Isoform —
PDB entities 7
Chains and sequence ranges Author chain M; PDBConstruct 1–162; UniProt 1–162

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id7kin
Deposition date deposition_date2020-10-23
Structure title titleMycobacterium tuberculosis WT RNAP transcription open promoter complex with WhiB7 promoter
Keywords keywordsRNA polymerase, Transcription factor, Mycobacterium tuberculosis, Iron cluster, TRANSCRIPTION, TRANSFERASE-DNA complex; TRANSCRIPTION, TRANSFERASE/DNA
Experimental Method methodELECTRON MICROSCOPY
▼

3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

7kin__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

7kin__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

7kin__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg(Guinier)51.98 Å
Rg (electron density)51.07 Å
Total Rg51.29 Å
Atom count29379
Residues3612
Excluded volume515170 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 7kin__assembly_1__model_1 decameric (10) Success 4.1.3-1-20251215 (887e7ef) View Download
▶

4. Crystallography and Experiment 4. Crystallography & Experiment

▶

5. Entities and Polymers Entities & Polymers (11)

▼

6. Fold Classification (SCOP + CATH) 3 domains

CATH v4.4 (3 domains)

Domain ID domain_id7kinB01
Class class2 — Mainly Beta
Architecture architecture170 — Beta Complex
Topology topology120 — RNA Polymerase Alpha Subunit; Chain A, domain 2
Homologous superfamily homologous superfamily12 — DNA-directed RNA polymerase, insert domain
Domain ID domain_id7kinD01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology274 — Enzyme I; Chain A, domain 2
Homologous superfamily homologous superfamily100 — RNA polymerase Rpb1, domain 3
Domain ID domain_id7kinD02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology132 — Topoisomerase I; Chain A, domain 4
Homologous superfamily homologous superfamily30 — RNA polymerase Rpb1 funnel domain
▶

7. Citations (1)