← Back to search
7m17

SN-407-LRRC8A in MSP1E3D1 lipid nanodiscs (Pose-1)

Method: ELECTRON MICROSCOPY Dmax: 129.9 Å Quality: GOOD
▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

Volume-regulated anion channel subunit LRRC8A

Mus musculus

UniProt Q80WG5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 6 PDB declaration: hexameric（6） Consistent with protein copy count Chain A； UniProt 1–810 Chain B； UniProt 1–810 Chain C； UniProt 1–810 Chain D； UniProt 1–810 Chain E； UniProt 1–810 Chain F； UniProt 1–810 Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 18 YNJ 7-{[(2S)-2-butyl-6,7-dichloro-2-cyclopentyl-1-oxo-2,3-dihydro-1H-inden-5-yl]oxy}heptanoic acid × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；pH to 7.4 with KOH cryo-EM vitrification conditions：Cryogen ETHANE；2 microliter drop size, manual wait time of 2 seconds, blot force of 1, 3 second blot time Resolution 3.65 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7M17 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6FNW Structure of a volume-regulated anion channel of the LRRC8 family Deposited 2018-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 398–810（413 aa）
Not recorded EDO 1,2-ETHANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；277.15 K；22% PEG 3350, 0.2M Na/K tartrate, 0.1M Bis-Tris propane pH 8.5
Resolution 1.80 Å R-free 0.208
6G8Z Structure of the pore domain of homomeric mLRRC8A volume-regulated anion channel at 3.66 A resolution Deposited 2018-04-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 1–810（810 aa）
Chain B 1–810（810 aa）
Chain C 1–810（810 aa）
Chain D 1–810（810 aa）
Chain E 1–810（810 aa）
Chain F 1–810（810 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.66 Å
6G9L Structure of homomeric mLRRC8A volume-regulated anion channel at 5.01 A resolution Deposited 2018-04-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 1–810（810 aa）
Chain B 1–810（810 aa）
Chain C 1–810（810 aa）
Chain D 1–810（810 aa）
Chain E 1–810（810 aa）
Chain F 1–810（810 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.01 Å
6G9O Structure of full-length homomeric mLRRC8A volume-regulated anion channel at 4.25 A resolution Deposited 2018-04-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 1–810（810 aa）
Chain B 1–810（810 aa）
Chain C 1–810（810 aa）
Chain D 1–810（810 aa）
Chain E 1–810（810 aa）
Chain F 1–810（810 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.25 Å
6NZW LRRC8A-DCPIB in MSP1E3D1 nanodisc constricted state Deposited 2019-02-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 1–810（810 aa）
Chain B 1–810（810 aa）
Chain C 1–810（810 aa）
Chain D 1–810（810 aa）
Chain E 1–810（810 aa）
Chain F 1–810（810 aa）
Not recorded POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 18 L9Y 4-{[(2S)-2-butyl-6,7-dichloro-2-cyclopentyl-1-oxo-2,3-dihydro-1H-inden-5-yl]oxy}butanoic acid × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 microliter drop size, 3 second blot time
Resolution 3.21 Å
6NZZ LRRC8A-DCPIB in MSP1E3D1 nanodisc expanded state Deposited 2019-02-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 1–810（810 aa）
Chain B 1–810（810 aa）
Chain C 1–810（810 aa）
Chain D 1–810（810 aa）
Chain E 1–810（810 aa）
Chain F 1–810（810 aa）
Not recorded POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 18 L9Y 4-{[(2S)-2-butyl-6,7-dichloro-2-cyclopentyl-1-oxo-2,3-dihydro-1H-inden-5-yl]oxy}butanoic acid × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 microliter drop size, 3 second blot time
Resolution 3.32 Å
6O00 apo-LRRC8A in MSP2N2 nanodisc constricted state Deposited 2019-02-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 1–810（810 aa）
Chain B 1–810（810 aa）
Chain C 1–810（810 aa）
Chain D 1–810（810 aa）
Chain E 1–810（810 aa）
Chain F 1–810（810 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 microliter drop size, 3 second blot time
Resolution 4.18 Å
7M19 SN-407-LRRC8A in MSP1E3D1 lipid nanodiscs (Pose-2) Deposited 2021-03-12 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 1–810（810 aa）
Chain B 1–810（810 aa）
Chain C 1–810（810 aa）
Chain D 1–810（810 aa）
Chain E 1–810（810 aa）
Chain F 1–810（810 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 18 YNJ 7-{[(2S)-2-butyl-6,7-dichloro-2-cyclopentyl-1-oxo-2,3-dihydro-1H-inden-5-yl]oxy}heptanoic acid × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；pH to 7.4 with KOH
cryo-EM vitrification conditions Cryogen ETHANE；2 microliter drop size, manual wait time of 2 seconds, blot force of 1, 3 second blot time
Resolution 3.69 Å
7P5V Structure of homomeric LRRC8A Volume-Regulated Anion Channel in complex with synthetic nanobody Sb1 Deposited 2021-07-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 15–808（794 aa）
Chain B 15–808（794 aa）
Chain C 15–808（794 aa）
Chain D 15–808（794 aa）
Chain E 15–808（794 aa）
Chain F 15–808（794 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.06 Å
7P5W Structure of homomeric LRRC8A Volume-Regulated Anion Channel in complex with synthetic nanobody Sb2 Deposited 2021-07-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 15–808（794 aa）
Chain B 15–808（794 aa）
Chain C 15–808（794 aa）
Chain D 15–808（794 aa）
Chain E 15–808（794 aa）
Chain F 15–808（794 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7P5Y Structure of homomeric LRRC8A Volume-Regulated Anion Channel in complex with synthetic nanobody Sb3 Deposited 2021-07-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 15–808（794 aa）
Chain B 15–808（794 aa）
Chain C 15–808（794 aa）
Chain D 15–808（794 aa）
Chain E 15–808（794 aa）
Chain F 15–808（794 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.29 Å
7P60 Structure of homomeric LRRC8A Volume-Regulated Anion Channel in complex with synthetic nanobody Sb4 at 1:0.5 ratio Deposited 2021-07-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 1–810（810 aa）
Chain B 1–810（810 aa）
Chain C 1–810（810 aa）
Chain D 1–810（810 aa）
Chain E 1–810（810 aa）
Chain F 1–810（810 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.80 Å
7P6K Structure of homomeric LRRC8A Volume-Regulated Anion Channel in complex with synthetic nanobody Sb5 Deposited 2021-07-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 1–810（810 aa）
Chain B 1–810（810 aa）
Chain C 1–810（810 aa）
Chain D 1–810（810 aa）
Chain E 1–810（810 aa）
Chain F 1–810（810 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.80 Å
8B41 Structure of heteromeric LRRC8A/C (1:1 co-transfected) Volume-Regulated Anion Channel in complex with synthetic nanobody Sb1 Deposited 2022-09-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain A 2–810（809 aa）
Chain B 2–810（809 aa）
Chain C 2–810（809 aa）
Chain D 2–810（809 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.80 Å
8B42 Structure of heteromeric LRRC8A/C Volume-Regulated Anion Channel. Deposited 2022-09-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 2–810（809 aa）
Chain B 2–810（809 aa）
Chain C 2–810（809 aa）
Chain D 2–810（809 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.60 Å
8DR8 LRRC8A:C conformation 2 (oblong) top mask Deposited 2022-07-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–71（71 aa）
Chain A 91–810（720 aa）
Chain B 1–71（71 aa）
Chain B 91–810（720 aa）
Chain C 1–71（71 aa）
Chain C 91–810（720 aa）
Chain D 1–71（71 aa）
Chain D 91–810（720 aa）
Chain E 1–71（71 aa）
Chain E 91–810（720 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 20 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8DRA LRRC8A:C conformation 2 (oblong) LRR mask Deposited 2022-07-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–71（71 aa）
Chain A 91–810（720 aa）
Chain B 1–71（71 aa）
Chain B 91–810（720 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.98 Å
8DRE LRRC8A:C conformation 2 (oblong) Deposited 2022-07-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–71（71 aa）
Chain A 91–810（720 aa）
Chain B 1–71（71 aa）
Chain B 91–810（720 aa）
Chain C 1–71（71 aa）
Chain C 91–810（720 aa）
Chain D 1–71（71 aa）
Chain D 91–810（720 aa）
Chain E 1–71（71 aa）
Chain E 91–810（720 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 20 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.18 Å
8DRK LRRC8A:C conformation 1 (round) top focus Deposited 2022-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–71（71 aa）
Chain A 91–810（720 aa）
Chain B 1–71（71 aa）
Chain B 91–810（720 aa）
Chain C 1–71（71 aa）
Chain C 91–810（720 aa）
Chain D 1–71（71 aa）
Chain D 91–810（720 aa）
Chain E 1–71（71 aa）
Chain E 91–810（720 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 21 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.95 Å
8DRN LRRC8A:C conformation 1 (round) LRR focus 1 Deposited 2022-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–71（71 aa）
Chain A 91–810（720 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.12 Å
8DRO LRRC8A:C conformation 1 (round) LRR focus 2 Deposited 2022-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 1–71（71 aa）
Chain B 91–810（720 aa）
Chain C 1–71（71 aa）
Chain C 91–810（720 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.06 Å
8DRQ LRRC8A:C conformation 1 (round) LRR focus 3 Deposited 2022-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain E 1–71（71 aa）
Chain E 91–810（720 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.16 Å
8DS3 LRRC8A:C conformation 1 (round) Deposited 2022-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–71（71 aa）
Chain A 91–810（720 aa）
Chain B 1–71（71 aa）
Chain B 91–810（720 aa）
Chain C 1–71（71 aa）
Chain C 91–810（720 aa）
Chain D 1–71（71 aa）
Chain D 91–810（720 aa）
Chain E 1–71（71 aa）
Chain E 91–810（720 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 21 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.07 Å
8DS9 LRRC8A:C in MSPE3D1 nanodisc top focus Deposited 2022-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–71（71 aa）
Chain A 91–810（720 aa）
Chain B 1–71（71 aa）
Chain B 91–810（720 aa）
Chain C 1–71（71 aa）
Chain C 91–810（720 aa）
Chain D 1–71（71 aa）
Chain D 91–810（720 aa）
Chain E 1–71（71 aa）
Chain E 91–810（720 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.17 Å
8DSA LRRC8A:C in MSP1E3D1 nanodisc Deposited 2022-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–71（71 aa）
Chain A 91–810（720 aa）
Chain B 1–71（71 aa）
Chain B 91–810（720 aa）
Chain C 1–71（71 aa）
Chain C 91–810（720 aa）
Chain D 1–71（71 aa）
Chain D 91–810（720 aa）
Chain E 1–71（71 aa）
Chain E 91–810（720 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
8F74 LRRC8A(T48D):C conformation 2 top focus Deposited 2022-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–76（76 aa）
Chain A 92–810（719 aa）
Chain B 1–76（76 aa）
Chain B 92–810（719 aa）
Chain C 1–76（76 aa）
Chain C 92–810（719 aa）
Chain D 1–76（76 aa）
Chain D 92–810（719 aa）
Chain E 1–76（76 aa）
Chain E 92–810（719 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 15 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8F75 LRRC8A(T48D):C conformation 2 LRR focus Deposited 2022-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 91–810（720 aa）
Chain B 91–810（720 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
8F77 LRRC8A(T48D):C conformation 2 top focus Deposited 2022-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–76（76 aa）
Chain A 90–810（721 aa）
Chain B 1–76（76 aa）
Chain B 90–810（721 aa）
Chain C 1–76（76 aa）
Chain C 90–810（721 aa）
Chain D 1–76（76 aa）
Chain D 90–810（721 aa）
Chain E 1–76（76 aa）
Chain E 90–810（721 aa）
Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 15 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.15 Å
8F79 LRRC8A(T48D):C conformation 2 top focus Deposited 2022-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–76（76 aa）
Chain A 90–810（721 aa）
Chain B 1–76（76 aa）
Chain B 90–810（721 aa）
Chain C 1–76（76 aa）
Chain C 90–810（721 aa）
Chain D 1–76（76 aa）
Chain D 90–810（721 aa）
Chain E 1–76（76 aa）
Chain E 90–810（721 aa）
Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 17 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.15 Å
8F7B LRRC8A(T48D):C conformation 2 top focus Deposited 2022-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–76（76 aa）
Chain A 90–810（721 aa）
Chain B 1–76（76 aa）
Chain B 90–810（721 aa）
Chain C 1–76（76 aa）
Chain C 90–810（721 aa）
Chain D 1–76（76 aa）
Chain D 90–810（721 aa）
Chain E 1–76（76 aa）
Chain E 90–810（721 aa）
Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 17 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.15 Å
8F7D LRRC8A(T48D):C conformation 2 top focus Deposited 2022-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–76（76 aa）
Chain A 90–810（721 aa）
Mutation：T48D Mutation：T48D No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8F7E LRRC8A(T48D):C conformation 2 top focus Deposited 2022-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 1–76（76 aa）
Chain B 90–810（721 aa）
Chain C 1–76（76 aa）
Chain C 90–810（721 aa）
Mutation：T48D Mutation：T48D Mutation：T48D Mutation：T48D No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
8F7J LRRC8A(T48D):C conformation 2 top focus Deposited 2022-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain E 1–76（76 aa）
Chain E 90–810（721 aa）
Mutation：T48D Mutation：T48D No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.32 Å
9DX7 LRRC8A:D Conformation 1 Deposited 2024-10-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–76（76 aa）
Chain A 91–810（720 aa）
Chain B 1–76（76 aa）
Chain B 91–810（720 aa）
Chain C 1–76（76 aa）
Chain C 91–810（720 aa）
Chain D 1–76（76 aa）
Chain D 91–810（720 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 18 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9DXA LRRC8A:D Conformation 2 Deposited 2024-10-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–76（76 aa）
Chain A 91–810（720 aa）
Chain B 1–76（76 aa）
Chain B 91–810（720 aa）
Chain C 1–76（76 aa）
Chain C 91–810（720 aa）
Chain D 1–76（76 aa）
Chain D 91–810（720 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 18 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å

35 other PDB entries and 35 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name LRC8A_MOUSE
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–810； UniProt 1–810 Author chain B； PDBConstruct 1–810； UniProt 1–810 Author chain C； PDBConstruct 1–810； UniProt 1–810 Author chain D； PDBConstruct 1–810； UniProt 1–810 Author chain E； PDBConstruct 1–810； UniProt 1–810 Author chain F； PDBConstruct 1–810； UniProt 1–810

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 7m17

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 7m17
Download Download
▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id7m17
Deposition date deposition_date2021-03-12
Structure title titleSN-407-LRRC8A in MSP1E3D1 lipid nanodiscs (Pose-1)
Keywords keywordsIon channel, Inhibitor, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method methodELECTRON MICROSCOPY
▼

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier42.96 Å
Radius of gyration Rg (electron density) rg_electron41.08 Å
Forward intensity I(0) i0641204000.00
Molecular weight molecular_weight227120.0 kDa
Excluded volume excluded_volume292070 ų
Envelope volume envelope_volume413550 ų
Hydration-shell volume shell_volume81796 ų
Envelope diameter envelope_diameter131.7 Å
Shell Rg shell_rg48.72 Å
Envelope Rg envelope_rg39.49 Å
Shape Rg shape_rg41.05 Å
Total Rg total_rg41.63 Å
Total atoms total_atoms16039
Residues n_residues1878
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
▼

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax129.9 Å
Rg (real space) rg_real42.71 Å
Rg uncertainty (real space) rg_real_error0.79 Å
I(0) (real space) i0_real6.4120e+08
I(0) uncertainty (real space) i0_real_error1.0070e+07
Rg (reciprocal space) rg_reciprocal42.96 Å
I(0) (reciprocal space) i0_reciprocal641400000.0000
Solution quality estimate total_estimate0.8320
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary56.2 Å
Skewness Skewness skewness0.076
Kurtosis Kurtosis kurtosis-0.528
Angular range angular_range— – 0.1850 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha83840000.0000
Real-space data points n_real_points38
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.946; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.973; Smooth: 0.000
▶

5. Crystallography and Experiment 5. Crystallography & Experiment

▶

6. Entities and Polymers Entities & Polymers (3)

▶

8. Citations (2)

▶

9. Files and Curves (10)