SAXSdb — 8aox

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1. Protein Identity and Related Structures Protein Identity & Related Structures

mRNA-capping enzyme nsP1

Chikungunya virus strain S27-African prototype

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 24	ZINC ION × 24 S-ADENOSYLMETHIONINE × 24 water × 24	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	POLN_CHIKS
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–535； UniProt 1–535 Author chain BA； PDBConstruct 1–535； UniProt 1–535 Author chain C； PDBConstruct 1–535； UniProt 1–535 Author chain DA； PDBConstruct 1–535； UniProt 1–535 Author chain E； PDBConstruct 1–535； UniProt 1–535 Author chain FA； PDBConstruct 1–535； UniProt 1–535 Author chain G； PDBConstruct 1–535； UniProt 1–535 Author chain HA； PDBConstruct 1–535； UniProt 1–535 Author chain I； PDBConstruct 1–535； UniProt 1–535 Author chain JA； PDBConstruct 1–535； UniProt 1–535 Author chain K； PDBConstruct 1–535； UniProt 1–535 Author chain LA； PDBConstruct 1–535； UniProt 1–535 Author chain M； PDBConstruct 1–535； UniProt 1–535 Author chain NA； PDBConstruct 1–535； UniProt 1–535 Author chain O； PDBConstruct 1–535； UniProt 1–535 Author chain PA； PDBConstruct 1–535； UniProt 1–535 Author chain Q； PDBConstruct 1–535； UniProt 1–535 Author chain RA； PDBConstruct 1–535； UniProt 1–535 Author chain S； PDBConstruct 1–535； UniProt 1–535 Author chain TA； PDBConstruct 1–535； UniProt 1–535 Author chain V； PDBConstruct 1–535； UniProt 1–535 Author chain VA； PDBConstruct 1–535； UniProt 1–535 Author chain X； PDBConstruct 1–535； UniProt 1–535 Author chain Z； PDBConstruct 1–535； UniProt 1–535

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	8aox
Deposition date deposition_date	2022-08-08
Structure title title	CryoEM structure of the Chikungunya virus nsP1 capping pores in complex with SAM
Keywords keywords	Alphavirus Replication complex Capping pores Membrane pore Methyltransferase gunayltransferase VIRAL PROTEIN, VIRAL PROTEIN; VIRAL PROTEIN
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8aox__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8aox__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

8aox__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	78.99 Å
Rg (electron density)	78.15 Å
Total Rg	78.32 Å
Atom count	81216
Residues	10368
Excluded volume	1446000 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`8aox__assembly_1__model_1`	24-meric （24）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Description description	mRNA-capping enzyme nsP1
Molecular weight formula_weight	59975.4 kDa
EC 编号 ec_number	2.1.1.-,2.7.7.-
Source method src_method	man
Copy count entity_count	24

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	JA,A,C,E,G,I,K,M,O,Q,S,V,X,Z,BA,DA,FA,HA,LA,NA,PA,RA,TA,VA
Atom count atom_count	87816

MDPVYVDIDADSAFLKALQRAYPMFEVEPRQVTPNDHANARAFSHLAIKLIEQEIDPDSTILDIGSAPARRMMSDRKYHCVCPMRSAEDPERLANYARKLASAAGKVLDRNISGKIGDLQAVMAVPDTETPTFCLHTDVSCRQRADVAIYQDVYAVHAPTSLYHQAIKGVRLAYWVGFDTTPFMYNAMAGAYPSYSTNWADEQVLKAKNIGLCSTDLTEGRRGKLSIMRGKKLEPCDRVLFSVGSTLYPESRKLLKSWHLPSVFHLKGKLSFTCRCDTVVSCEGYVVKRITMSPGLYGKTTGYAVTHHADGFLMCKTTDTVDGERVSFSVCTYVPATICDQMTGILATEVTPEDAQKLLVGLNQRIVVNGRTQRNTNTMKNYMIPVVAQAFSKWAKECRKDMEDEKLLGVRERTLTCCCLWAFKKQKTHTVYKRPDTQSIQKVQAEFDSFVVPSLWSSGLSIPLRTRIKWLLSKVPKTDLTPYSGDAQEARDAEKEAEEEREAELTLEALPPLQAAQEDVQVEIDVEQLEDRAGA

Source Source

Source organism organism	Chikungunya virus strain S27-African prototype
NCBI taxonomy ID ncbi_taxonomy_id	371094
Host organism host_organism	Baculovirus expression vector pFastBac1-HM

Entity 2 Entity 2 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	24

Entity 3 Entity 3 non-polymer

Description description	S-ADENOSYLMETHIONINE
Molecular weight formula_weight	398.4 kDa
Source method src_method	syn
Copy count entity_count	24

Entity 4 Entity 4 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	4848

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	triclinic

Resolution resolution	2.80 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	69057

CryoEM structure of the Chikungunya virus nsP1 capping pores in complex with SAM

1. Protein Identity and Related Structures Protein Identity & Related Structures

mRNA-capping enzyme nsP1

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

8aox__assembly_1__model_1

8aox__assembly_1__model_1 | I(q)

8aox__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 water

7. Citations (1)

CryoEM structure of the Chikungunya virus nsP1 capping pores in complex with SAM

1. Protein Identity and Related Structures Protein Identity & Related Structures

mRNA-capping enzyme nsP1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

8aox__assembly_1__model_1

8aox__assembly_1__model_1 | I(q)

8aox__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 water

7. Citations (1)