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8e32

E. coli 50S ribosome bound to compound streptogramin analogs SA1 and SB1

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

50S ribosomal protein L2

Escherichia coli

UniProt P60422

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 8 RNA 2 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L15 × 1 （A0A037Y8L6） 50S ribosomal protein L4 × 1 （A0A7U9FY55） 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L13 × 1 （S1EW51） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L32 × 1 （A0A828UBL8） 50S ribosomal protein L34 × 1 （B7MGC4） ;(3R,4R,5E,10E,12E,14S,16R,23S,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-yn-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione ; × 1 ;~{N}-[(3~{S},6~{S},12~{R},15~{S},16~{R},19~{S},22~{S},24~{R})-12-[2-[(azanylidene-$l^{4}-azanylidene)amino]ethyl]-4,16-dimethyl-24-oxidanyl-2,5,11,14,18,21-hexakis(oxidanylidene)-19-phenyl-3-(phenylmethyl)-17-oxa-1,4,10,13,20-pentazatricyclo[20.4.0.0^{6,10}]hexacosan-15-yl]-3-oxidanyl-pyridine-2-carboxamide ; × 1 water × 1 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
10qm 11bq 11dg 11el 11fv 11gg 28uh 28ui 28uj 28uk 28ul 28um

670 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name RL2_ECOLI
Isoform —
PDB entities 3
Chains and sequence ranges Author chain K； PDBConstruct 1–271； UniProt 2–272

50S ribosomal protein L15

Escherichia coli

UniProt A0A037Y8L6

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 8 RNA 2 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （P60422） 50S ribosomal protein L4 × 1 （A0A7U9FY55） 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L13 × 1 （S1EW51） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L32 × 1 （A0A828UBL8） 50S ribosomal protein L34 × 1 （B7MGC4） ;(3R,4R,5E,10E,12E,14S,16R,23S,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-yn-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione ; × 1 ;~{N}-[(3~{S},6~{S},12~{R},15~{S},16~{R},19~{S},22~{S},24~{R})-12-[2-[(azanylidene-$l^{4}-azanylidene)amino]ethyl]-4,16-dimethyl-24-oxidanyl-2,5,11,14,18,21-hexakis(oxidanylidene)-19-phenyl-3-(phenylmethyl)-17-oxa-1,4,10,13,20-pentazatricyclo[20.4.0.0^{6,10}]hexacosan-15-yl]-3-oxidanyl-pyridine-2-carboxamide ; × 1 water × 1 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
6lkq 6o9j 6o9k 6ogf 6ogg 6ogi 6om6 6ore 6orl 6osk 6osq 6ost

147 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name A0A037Y8L6_ECOLX
Isoform —
PDB entities 4
Chains and sequence ranges Author chain L； PDBConstruct 1–144； UniProt 1–144

50S ribosomal protein L4

Escherichia coli

UniProt A0A7U9FY55

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 8 RNA 2 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （P60422） 50S ribosomal protein L15 × 1 （A0A037Y8L6） 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L13 × 1 （S1EW51） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L32 × 1 （A0A828UBL8） 50S ribosomal protein L34 × 1 （B7MGC4） ;(3R,4R,5E,10E,12E,14S,16R,23S,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-yn-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione ; × 1 ;~{N}-[(3~{S},6~{S},12~{R},15~{S},16~{R},19~{S},22~{S},24~{R})-12-[2-[(azanylidene-$l^{4}-azanylidene)amino]ethyl]-4,16-dimethyl-24-oxidanyl-2,5,11,14,18,21-hexakis(oxidanylidene)-19-phenyl-3-(phenylmethyl)-17-oxa-1,4,10,13,20-pentazatricyclo[20.4.0.0^{6,10}]hexacosan-15-yl]-3-oxidanyl-pyridine-2-carboxamide ; × 1 water × 1 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
7uph 8e30 8e32 8e33 8e35 8e36 8e3l 8e3m 8e3o 8e41 8e42 8e43

25 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name A0A7U9FY55_ECOLX
Isoform —
PDB entities 5
Chains and sequence ranges Author chain M； PDBConstruct 1–201； UniProt 1–201

50S ribosomal protein L3

Escherichia coli

UniProt P60438

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 8 RNA 2 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （P60422） 50S ribosomal protein L15 × 1 （A0A037Y8L6） 50S ribosomal protein L4 × 1 （A0A7U9FY55） 50S ribosomal protein L13 × 1 （S1EW51） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L32 × 1 （A0A828UBL8） 50S ribosomal protein L34 × 1 （B7MGC4） ;(3R,4R,5E,10E,12E,14S,16R,23S,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-yn-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione ; × 1 ;~{N}-[(3~{S},6~{S},12~{R},15~{S},16~{R},19~{S},22~{S},24~{R})-12-[2-[(azanylidene-$l^{4}-azanylidene)amino]ethyl]-4,16-dimethyl-24-oxidanyl-2,5,11,14,18,21-hexakis(oxidanylidene)-19-phenyl-3-(phenylmethyl)-17-oxa-1,4,10,13,20-pentazatricyclo[20.4.0.0^{6,10}]hexacosan-15-yl]-3-oxidanyl-pyridine-2-carboxamide ; × 1 water × 1 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
10qm 11fv 11gg 28uh 28ui 28uj 28uk 28ul 28um 28un 2j28 2rdo

675 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name RL3_ECOLI
Isoform —
PDB entities 6
Chains and sequence ranges Author chain N； PDBConstruct 1–209； UniProt 1–209

50S ribosomal protein L13

Escherichia coli

UniProt S1EW51

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 8 RNA 2 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （P60422） 50S ribosomal protein L15 × 1 （A0A037Y8L6） 50S ribosomal protein L4 × 1 （A0A7U9FY55） 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L32 × 1 （A0A828UBL8） 50S ribosomal protein L34 × 1 （B7MGC4） ;(3R,4R,5E,10E,12E,14S,16R,23S,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-yn-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione ; × 1 ;~{N}-[(3~{S},6~{S},12~{R},15~{S},16~{R},19~{S},22~{S},24~{R})-12-[2-[(azanylidene-$l^{4}-azanylidene)amino]ethyl]-4,16-dimethyl-24-oxidanyl-2,5,11,14,18,21-hexakis(oxidanylidene)-19-phenyl-3-(phenylmethyl)-17-oxa-1,4,10,13,20-pentazatricyclo[20.4.0.0^{6,10}]hexacosan-15-yl]-3-oxidanyl-pyridine-2-carboxamide ; × 1 water × 1 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
6lkq 7cpj 7s1i 7s1j 7s1k 7tos 7ug7 7uph 8e30 8e32 8e33 8e35

79 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name S1EW51_ECOLX
Isoform —
PDB entities 7
Chains and sequence ranges Author chain O； PDBConstruct 1–142； UniProt 1–142

50S ribosomal protein L22

Escherichia coli

UniProt P61175

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 8 RNA 2 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （P60422） 50S ribosomal protein L15 × 1 （A0A037Y8L6） 50S ribosomal protein L4 × 1 （A0A7U9FY55） 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L13 × 1 （S1EW51） 50S ribosomal protein L32 × 1 （A0A828UBL8） 50S ribosomal protein L34 × 1 （B7MGC4） ;(3R,4R,5E,10E,12E,14S,16R,23S,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-yn-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione ; × 1 ;~{N}-[(3~{S},6~{S},12~{R},15~{S},16~{R},19~{S},22~{S},24~{R})-12-[2-[(azanylidene-$l^{4}-azanylidene)amino]ethyl]-4,16-dimethyl-24-oxidanyl-2,5,11,14,18,21-hexakis(oxidanylidene)-19-phenyl-3-(phenylmethyl)-17-oxa-1,4,10,13,20-pentazatricyclo[20.4.0.0^{6,10}]hexacosan-15-yl]-3-oxidanyl-pyridine-2-carboxamide ; × 1 water × 1 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
10qm 11bq 11dg 11el 11fv 11gg 28uh 28ui 28uj 28uk 28ul 28um

617 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name RL22_ECOLI
Isoform —
PDB entities 8
Chains and sequence ranges Author chain P； PDBConstruct 1–110； UniProt 1–110

50S ribosomal protein L32

Escherichia coli

UniProt A0A828UBL8

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 8 RNA 2 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （P60422） 50S ribosomal protein L15 × 1 （A0A037Y8L6） 50S ribosomal protein L4 × 1 （A0A7U9FY55） 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L13 × 1 （S1EW51） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L34 × 1 （B7MGC4） ;(3R,4R,5E,10E,12E,14S,16R,23S,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-yn-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione ; × 1 ;~{N}-[(3~{S},6~{S},12~{R},15~{S},16~{R},19~{S},22~{S},24~{R})-12-[2-[(azanylidene-$l^{4}-azanylidene)amino]ethyl]-4,16-dimethyl-24-oxidanyl-2,5,11,14,18,21-hexakis(oxidanylidene)-19-phenyl-3-(phenylmethyl)-17-oxa-1,4,10,13,20-pentazatricyclo[20.4.0.0^{6,10}]hexacosan-15-yl]-3-oxidanyl-pyridine-2-carboxamide ; × 1 water × 1 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
10qm 7uph 8e30 8e32 8e33 8e35 8e36 8e3l 8e3m 8e3o 8e41 8e42

41 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name A0A828UBL8_ECOLX
Isoform —
PDB entities 9
Chains and sequence ranges Author chain Q； PDBConstruct 1–56； UniProt 2–57

50S ribosomal protein L34

Escherichia coli

UniProt B7MGC4

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 8 RNA 2 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （P60422） 50S ribosomal protein L15 × 1 （A0A037Y8L6） 50S ribosomal protein L4 × 1 （A0A7U9FY55） 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L13 × 1 （S1EW51） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L32 × 1 （A0A828UBL8） ;(3R,4R,5E,10E,12E,14S,16R,23S,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-yn-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione ; × 1 ;~{N}-[(3~{S},6~{S},12~{R},15~{S},16~{R},19~{S},22~{S},24~{R})-12-[2-[(azanylidene-$l^{4}-azanylidene)amino]ethyl]-4,16-dimethyl-24-oxidanyl-2,5,11,14,18,21-hexakis(oxidanylidene)-19-phenyl-3-(phenylmethyl)-17-oxa-1,4,10,13,20-pentazatricyclo[20.4.0.0^{6,10}]hexacosan-15-yl]-3-oxidanyl-pyridine-2-carboxamide ; × 1 water × 1 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
10qm 11gg 5u9f 5u9g 5wf0 5wfs 6dnc 6o9j 6o9k 6ogf 6ogg 6ogi

172 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name RL34_ECO45
Isoform —
PDB entities 10
Chains and sequence ranges Author chain R； PDBConstruct 1–46； UniProt 1–46

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id8e32
Deposition date deposition_date2022-08-16
Structure title titleE. coli 50S ribosome bound to compound streptogramin analogs SA1 and SB1
Keywords keywordsE. coli ribosome, streptogramin A, streptogramin B, antibiotics, RIBOSOME-ANTIBIOTIC complex; RIBOSOME/ANTIBIOTIC
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8e32__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8e32__assembly_1__model_1 | I(q)

10-2 10-1 107 108 109 1010 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

8e32__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）70.06 Å
Rg (electron density)69.71 Å
Total Rg69.85 Å
Atom count116061
Residues4174
Excluded volume1076500 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 8e32__assembly_1__model_1 decameric （10） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (13)

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7. Citations (1)