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8h37

Cryo-EM Structure of the KBTBD2-CUL3-Rbx1-p85a tetrameric complex

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Cullin-3

Homo sapiens

UniProt Q13618

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 16 E3 ubiquitin-protein ligase RBX1 × 4 （P62877） Kelch repeat and BTB domain-containing protein 2 × 4 （Q8IY47） Phosphatidylinositol 3-kinase regulatory subunit alpha × 4 （P27986） ZINC ION × 12 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
2myl 2mym

2 assemblies

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
4ap2 4apf 4eoz 4eoz 4eoz 4hxi 5nlb 6i2m 8gq6 8h33 8h34 8h35

30 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name CUL3_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain C； PDBConstruct 1–768； UniProt 1–768 Author chain F； PDBConstruct 1–768； UniProt 1–768 Author chain M； PDBConstruct 1–768； UniProt 1–768 Author chain O； PDBConstruct 1–768； UniProt 1–768

E3 ubiquitin-protein ligase RBX1

Homo sapiens

UniProt P62877

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 16 Cullin-3 × 4 （Q13618） Kelch repeat and BTB domain-containing protein 2 × 4 （Q8IY47） Phosphatidylinositol 3-kinase regulatory subunit alpha × 4 （P27986） ZINC ION × 12 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
2lgv

1 assemblies

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1ldj 1ldk 1ldk 1u6g 29hg 29hh 29hi 2hye 3dpl 3dqv 3dqv 3dqv

107 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name RBX1_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain D； PDBConstruct 1–108； UniProt 1–108 Author chain E； PDBConstruct 1–108； UniProt 1–108 Author chain Q； PDBConstruct 1–108； UniProt 1–108 Author chain R； PDBConstruct 1–108； UniProt 1–108

Kelch repeat and BTB domain-containing protein 2

Homo sapiens

UniProt Q8IY47

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 16 Cullin-3 × 4 （Q13618） E3 ubiquitin-protein ligase RBX1 × 4 （P62877） Phosphatidylinositol 3-kinase regulatory subunit alpha × 4 （P27986） ZINC ION × 12 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
8gq6 8h33 8h34 8h35 8h36 8h37 8h38 8h3a 8h3f 8h3r

10 assemblies

View Construct and Data Evidence
UniProt name KBTB2_HUMAN
Isoform —
PDB entities 3
Chains and sequence ranges Author chain A； PDBConstruct 1–623； UniProt 1–623 Author chain B； PDBConstruct 1–623； UniProt 1–623 Author chain N； PDBConstruct 1–623； UniProt 1–623 Author chain P； PDBConstruct 1–623； UniProt 1–623

Phosphatidylinositol 3-kinase regulatory subunit alpha

Homo sapiens

UniProt P27986

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 16 Cullin-3 × 4 （Q13618） E3 ubiquitin-protein ligase RBX1 × 4 （P62877） Kelch repeat and BTB domain-containing protein 2 × 4 （Q8IY47） ZINC ION × 12 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
1pbw 1pbw 1pht 1pks 1pkt 2iug 3i5s 3i5s 3i5s 3i5s 5vlr

11 assemblies

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1a0n 1azg 1h9o 1pic 2iuh 2iui 2iui 2rd0 2v1y 3hhm 3hiz 3i5r

106 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name P85A_HUMAN
Isoform —
PDB entities 4
Chains and sequence ranges Author chain G； PDBConstruct 1–724； UniProt 1–724 Author chain H； PDBConstruct 1–724； UniProt 1–724 Author chain I； PDBConstruct 1–724； UniProt 1–724 Author chain J； PDBConstruct 1–724； UniProt 1–724

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id8h37
Deposition date deposition_date2022-10-08
Structure title titleCryo-EM Structure of the KBTBD2-CUL3-Rbx1-p85a tetrameric complex
Keywords keywordsligase, complex; LIGASE
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8h37__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8h37__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

8h37__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）82.26 Å
Rg (electron density)81.69 Å
Total Rg81.75 Å
Atom count49321
Residues6160
Excluded volume875840 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 8h37__assembly_1__model_1 hexadecameric （16） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (5)

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7. Citations (1)