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8j6m

SIDT1 protein

Method: ELECTRON MICROSCOPY Dmax: 127.0 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Green fluorescent protein,SID1 transmembrane family member 1

Homo sapiens

UniProt A0A059PIQ0

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Insufficient information Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 3–236 Chain B； UniProt 3–236 Not recorded CLR CHOLESTEROL × 8 ZN ZINC ION × 2 NA SODIUM ION × 1 OLA OLEIC ACID × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.77 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8J6M Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
28QL GFP bound to distal DARPin (AHIR dodecamer scaffold system) Deposited 2026-02-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 3–229（227 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.72 Å
5BT0 Switching GFP fluorescence using genetically encoded phenyl azide chemistry through two different non-native post-translational modifications routes at the same position. Deposited 2015-06-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–234（232 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；297 K；50 mM MMT, 2.5 M (NH4)2SO4
Resolution 2.03 Å R-free 0.204
5BT0 Switching GFP fluorescence using genetically encoded phenyl azide chemistry through two different non-native post-translational modifications routes at the same position. Deposited 2015-06-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 3–234（232 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；297 K；50 mM MMT, 2.5 M (NH4)2SO4
Resolution 2.03 Å R-free 0.204
5BTT Switching GFP fluorescence using genetically encoded phenyl azide chemistry through two different non-native post-translational modifications routes at the same position. Deposited 2015-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–231（229 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 5 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；297 K；50 mM MMT, 2.5 M (NH4)2SO4
Resolution 2.14 Å R-free 0.207
5BTT Switching GFP fluorescence using genetically encoded phenyl azide chemistry through two different non-native post-translational modifications routes at the same position. Deposited 2015-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 3–231（229 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 6 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；297 K；50 mM MMT, 2.5 M (NH4)2SO4
Resolution 2.14 Å R-free 0.207
5DPG sfGFP mutant - 133 p-cyano-L-phenylalanine Deposited 2015-09-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–238（238 aa）
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, D133(4CF), A206V Non-standard monomer：Yes (specific site not provided by mmCIF) EDO 1,2-ETHANEDIOL × 10 NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；22% PEG 3350, 2% Tacsimate pH 6, 0.1 M Bis-Tris pH 6.5
Resolution 1.85 Å R-free 0.192
5DPH sfGFP mutant - 149 p-cyano-L-phenylalanine Deposited 2015-09-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–238（238 aa）
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, A206V, N149(4CF) Non-standard monomer：Yes (specific site not provided by mmCIF) CO2 CARBON DIOXIDE × 1 MG MAGNESIUM ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；0.1 M Tris pH 8.5, 20% PEG 4000, 0.21 M MgCl2-6H2O
Resolution 1.42 Å R-free 0.208
5DPH sfGFP mutant - 149 p-cyano-L-phenylalanine Deposited 2015-09-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–238（238 aa）
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, A206V, N149(4CF) Non-standard monomer：Yes (specific site not provided by mmCIF) CO2 CARBON DIOXIDE × 1 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；0.1 M Tris pH 8.5, 20% PEG 4000, 0.21 M MgCl2-6H2O
Resolution 1.42 Å R-free 0.208
5DPI sfGFP double mutant - 133/149 p-cyano-L-phenylalanine Deposited 2015-09-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–238（238 aa）
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, A206V, D133(4CF), N149(4CF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；0.2 M potassium sodium formate, 30% PEG 3350
Resolution 2.54 Å R-free 0.243
5DPI sfGFP double mutant - 133/149 p-cyano-L-phenylalanine Deposited 2015-09-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–238（238 aa）
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, A206V, D133(4CF), N149(4CF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；0.2 M potassium sodium formate, 30% PEG 3350
Resolution 2.54 Å R-free 0.243
5DPI sfGFP double mutant - 133/149 p-cyano-L-phenylalanine Deposited 2015-09-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 1–238（238 aa）
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, A206V, D133(4CF), N149(4CF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；0.2 M potassium sodium formate, 30% PEG 3350
Resolution 2.54 Å R-free 0.243
5DPI sfGFP double mutant - 133/149 p-cyano-L-phenylalanine Deposited 2015-09-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 1–238（238 aa）
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, A206V, D133(4CF), N149(4CF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；0.2 M potassium sodium formate, 30% PEG 3350
Resolution 2.54 Å R-free 0.243
5DPI sfGFP double mutant - 133/149 p-cyano-L-phenylalanine Deposited 2015-09-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain E 1–238（238 aa）
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, A206V, D133(4CF), N149(4CF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；0.2 M potassium sodium formate, 30% PEG 3350
Resolution 2.54 Å R-free 0.243
5DPI sfGFP double mutant - 133/149 p-cyano-L-phenylalanine Deposited 2015-09-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain F 1–238（238 aa）
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, A206V, D133(4CF), N149(4CF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；0.2 M potassium sodium formate, 30% PEG 3350
Resolution 2.54 Å R-free 0.243
5DPJ sfGFP double mutant - 133/149 p-ethynyl-L-phenylalanine Deposited 2015-09-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–238（238 aa）
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, D133(5DW), N149(5DW), A206V Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；22% PEG 3350 and 0.20 M sodium citrate tribasic, pH 7
Resolution 2.50 Å R-free 0.244
5DPJ sfGFP double mutant - 133/149 p-ethynyl-L-phenylalanine Deposited 2015-09-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–238（238 aa）
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, D133(5DW), N149(5DW), A206V Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；22% PEG 3350 and 0.20 M sodium citrate tribasic, pH 7
Resolution 2.50 Å R-free 0.244
5DPJ sfGFP double mutant - 133/149 p-ethynyl-L-phenylalanine Deposited 2015-09-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 1–238（238 aa）
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, D133(5DW), N149(5DW), A206V Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；22% PEG 3350 and 0.20 M sodium citrate tribasic, pH 7
Resolution 2.50 Å R-free 0.244
5DPJ sfGFP double mutant - 133/149 p-ethynyl-L-phenylalanine Deposited 2015-09-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 1–238（238 aa）
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, D133(5DW), N149(5DW), A206V Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；22% PEG 3350 and 0.20 M sodium citrate tribasic, pH 7
Resolution 2.50 Å R-free 0.244
5DY6 Enhanced superfolder GFP with DBCO at 148 Deposited 2015-09-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 5–232（228 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；294 K；0.1 M PCTP Buffer, 15% PEG 1500
Resolution 2.66 Å R-free 0.290
5DY6 Enhanced superfolder GFP with DBCO at 148 Deposited 2015-09-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 5–232（228 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；294 K；0.1 M PCTP Buffer, 15% PEG 1500
Resolution 2.66 Å R-free 0.290
5EHU sfGFP mutant with unnatural amino acid 4-azidoethoxy-L-phenylalanine incorporated at the 149 site Deposited 2015-10-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–238（237 aa）
Mutation：S30R, A72S, Q80R, N149(4LZ), A206V Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；A 40 mg/mL solution of sfGFP-149-AePhe in a 20 mM Hepes buffer pH 7.5 was combined with a precipitation solution (20% PEG 8000, 100 mM Hepes pH 7.5) in a 1:1 ratio to form crystals in a sitting drop well at room temperature
Resolution 1.45 Å R-free 0.208
5EHU sfGFP mutant with unnatural amino acid 4-azidoethoxy-L-phenylalanine incorporated at the 149 site Deposited 2015-10-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 2–238（237 aa）
Mutation：S30R, A72S, Q80R, N149(4LZ), A206V Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；A 40 mg/mL solution of sfGFP-149-AePhe in a 20 mM Hepes buffer pH 7.5 was combined with a precipitation solution (20% PEG 8000, 100 mM Hepes pH 7.5) in a 1:1 ratio to form crystals in a sitting drop well at room temperature
Resolution 1.45 Å R-free 0.208
5JZK The Structure of Ultra Stable Green Fluorescent Protein Deposited 2016-05-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 3–238（236 aa） Fragment：UNP residues 3-238
Chain B 3–238（236 aa） Fragment：UNP residues 3-238
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) NO3 NITRATE ION × 4 EDO 1,2-ETHANEDIOL × 2 CL CHLORIDE ION × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；281 K；20 % w/v PEG 3350, 0.1 M Bis-Tris propane pH 6.5, 0.2 M Sodium nitrate
Resolution 1.90 Å R-free 0.176
5JZL The Structure of Monomeric Ultra Stable Green Fluorescent Protein Deposited 2016-05-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa） Fragment：UNP residues 3-238
Non-standard monomer：Yes (specific site not provided by mmCIF) CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；281 K；22% w/v PEG 8000, 0.2 M sodium chloride, 0.1 M phosphate-citrate buffer pH 3.7, 4% v/v acetone
Resolution 1.80 Å R-free 0.217
5JZL The Structure of Monomeric Ultra Stable Green Fluorescent Protein Deposited 2016-05-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 3–238（236 aa） Fragment：UNP residues 3-238
Non-standard monomer：Yes (specific site not provided by mmCIF) CL CHLORIDE ION × 1 NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；281 K；22% w/v PEG 8000, 0.2 M sodium chloride, 0.1 M phosphate-citrate buffer pH 3.7, 4% v/v acetone
Resolution 1.80 Å R-free 0.217
5MFC Designed armadillo repeat protein YIIIM5AII in complex with (KR)4-GFP Deposited 2016-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) ACT ACETATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；277 K；8% w/v PEG 4000, 0.1M Na acetate trihydrate pH 4.6,
Resolution 2.40 Å R-free 0.240
5MFC Designed armadillo repeat protein YIIIM5AII in complex with (KR)4-GFP Deposited 2016-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；277 K；8% w/v PEG 4000, 0.1M Na acetate trihydrate pH 4.6,
Resolution 2.40 Å R-free 0.240
5NHN Super-Folder Green Fluorescent Protein Artificiall dimer linked via 148 position Deposited 2017-03-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 3–231（229 aa）
Chain B 3–231（229 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) DB5 [(4~{S})-4,5,6,7,8,9-hexahydro-1~{H}-cycloocta[d][1,2,3]triazol-4-yl] hydrogen carbonate × 1 GOL GLYCEROL × 1 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；300 K；Condition C6 of the PACT Premier screen: 0.1 M PCTP Buffer, pH 9.0, 25% PEG 1500
Resolution 1.96 Å R-free 0.209
5NI3 sfGFP 204-204 mutant dimer Deposited 2017-03-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 3–229（227 aa）
Chain D 3–233（231 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) NA SODIUM ION × 6 DB5 [(4~{S})-4,5,6,7,8,9-hexahydro-1~{H}-cycloocta[d][1,2,3]triazol-4-yl] hydrogen carbonate × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；PACT condition C6: 0.1M PCTP Buffer, pH 9.0, 25% PEG 1500
Resolution 1.28 Å R-free 0.191
5NI3 sfGFP 204-204 mutant dimer Deposited 2017-03-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 3–233（231 aa）
Chain C 3–233（231 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) NA SODIUM ION × 4 DB5 [(4~{S})-4,5,6,7,8,9-hexahydro-1~{H}-cycloocta[d][1,2,3]triazol-4-yl] hydrogen carbonate × 1 GOL GLYCEROL × 4 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；PACT condition C6: 0.1M PCTP Buffer, pH 9.0, 25% PEG 1500
Resolution 1.28 Å R-free 0.191
5YR2 Structure of cpGFP66BPA Deposited 2017-11-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 146–235（90 aa）
Chain A 3–144（142 aa）
Mutation：Y163(BPA) Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：Y163(BPA) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；289.15 K；PEG 3350 25%, 0.2M MgCl2,0.1M Bis-Tris pH 5.5
Resolution 1.80 Å R-free 0.215
5YR2 Structure of cpGFP66BPA Deposited 2017-11-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 146–235（90 aa）
Chain B 3–144（142 aa）
Mutation：Y163(BPA) Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：Y163(BPA) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；289.15 K；PEG 3350 25%, 0.2M MgCl2,0.1M Bis-Tris pH 5.5
Resolution 1.80 Å R-free 0.215
5Z6Y Structure of sfYFP48S95C66BPA Deposited 2018-01-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–230（228 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；289 K；0.1M sodium malonate pH 4.0, 12% PEG 3350
Resolution 1.87 Å R-free 0.236
6B9C Superfolder Green Fluorescent Protein with 4-nitro-L-phenylalanine at the chromophore (position 66) Deposited 2017-10-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Mutation：S30R, A72S, Q80R, A206V Non-standard monomer：Yes (specific site not provided by mmCIF) CO2 CARBON DIOXIDE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；1.4 M DL-Malic acid
Resolution 1.70 Å R-free 0.195
6B9C Superfolder Green Fluorescent Protein with 4-nitro-L-phenylalanine at the chromophore (position 66) Deposited 2017-10-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 3–238（236 aa）
Mutation：S30R, A72S, Q80R, A206V Non-standard monomer：Yes (specific site not provided by mmCIF) CO2 CARBON DIOXIDE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；1.4 M DL-Malic acid
Resolution 1.70 Å R-free 0.195
6H01 Crystal structure of a domain-swapped dark-state sfGFP containing the unnatural amino acid ortho-nitrobenzyl-tyrosine (ONBY) at residue 66 Deposited 2018-07-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 3–238（236 aa）
Chain C 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；291 K；1.1 M tri-Na-citrate 0.1 M Na-HEPES
Resolution 2.78 Å R-free 0.239
6H01 Crystal structure of a domain-swapped dark-state sfGFP containing the unnatural amino acid ortho-nitrobenzyl-tyrosine (ONBY) at residue 66 Deposited 2018-07-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 3–238（236 aa）
Chain D 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；291 K；1.1 M tri-Na-citrate 0.1 M Na-HEPES
Resolution 2.78 Å R-free 0.239
6HUT GFP8 - a stabilized variant of cycle-3 GFP Deposited 2018-10-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–245（245 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；296 K；200 mM HEPES, 10% v/v isopropanol, 200 mM NaCl
Resolution 1.29 Å R-free 0.203
6KRG Crystal structure of sfGFP Y182TMSiPhe Deposited 2019-08-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–236（236 aa）
Mutation：Y182T Non-standard monomer：Yes (specific site not provided by mmCIF) GOL GLYCEROL × 2 CL CHLORIDE ION × 3 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；289 K；PEG 6000, SODIUM CHLORIDE
Resolution 1.40 Å R-free 0.173
6OA8 Superfolder Green Fluorescent Protein with 4-cyano-L-phenylalanine at the chromophore (position 66) Deposited 2019-03-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) EDO 1,2-ETHANEDIOL × 3 SO4 SULFATE ION × 8 NA SODIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；295 K；0.1 M Tris-HCl, 2.0 M ammonium sulfate, pH 8.5
Resolution 1.37 Å R-free 0.189
6UN5 Crystal structure of green fluorescent protein (GFP); S65T, Y66(2,3,5-F3Y); ih circular permutant (50-51) Deposited 2019-10-10 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 51–237（187 aa）
Chain B 51–237（187 aa）
Mutation：;A72S, Q80R, T105K, E111V, I128T, K166T, I167V, S205T, A206V, S232R, Y241I, C250S,A72S, Q80R, T105K, E111V, I128T, K166T, I167V, S205T, A206V ; Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：;A72S, Q80R, T105K, E111V, I128T, K166T, I167V, S205T, A206V, S232R, Y241I, C250S,A72S, Q80R, T105K, E111V, I128T, K166T, I167V, S205T, A206V ; Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；0.15 M ammonium acetate, 31% PEG 3350
Resolution 1.36 Å R-free 0.205
6UN6 Crystal structure of green fluorescent protein (GFP); S65T, Y66(3-NO2Y); ih circular permutant (50-51) Deposited 2019-10-10 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 51–237（187 aa）
Chain B 51–237（187 aa）
Mutation：A72S, Q80R, T105K, E111V, I128T, K166T, I167V, S205T, A206V, S232R, Y241I, C250S Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：A72S, Q80R, T105K, E111V, I128T, K166T, I167V, S205T, A206V, S232R, Y241I, C250S Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；0.15 M ammonium acetate, 34% PEG 3350
Resolution 1.50 Å R-free 0.181
6UN7 Crystal structure of green fluorescent protein (GFP); S65T, Y66(3-OMeY); ih circular permutant (50-51) Deposited 2019-10-10 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 51–237（187 aa）
Chain B 51–237（187 aa）
Mutation：A72S, Q80R, T105K, E111V, I128T, K166T, I167V, S205T, A206V, S232R, Y241I, C250S Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：A72S, Q80R, T105K, E111V, I128T, K166T, I167V, S205T, A206V, S232R, Y241I, C250S Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；0.15 M ammonium acetate, 34% PEG 3350
Resolution 1.50 Å R-free 0.191
6WV3 Human VKOR with warfarin Deposited 2020-05-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–144（144 aa） Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Chain A 146–231（86 aa） Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 3 SWF S-WARFARIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 6.5；295 K；37% PEG400, 0.1 M MES, pH 6.5
Resolution 2.20 Å R-free 0.223
6WV4 Human VKOR C43S with warfarin Deposited 2020-05-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–144（144 aa） Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Chain A 146–231（86 aa） Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Mutation：C43S Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：C43S Non-standard monomer：Yes (specific site not provided by mmCIF) SWF S-WARFARIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 6.5；295 K；25-33% PEG400, 185-220 mM potassium formate, 0.1 M MES, pH 6.5
Resolution 3.01 Å R-free 0.292
6WV5 Human VKOR C43S mutant with vitamin K1 epoxide Deposited 2020-05-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–144（144 aa） Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Chain A 146–231（86 aa） Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Mutation：C43S Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：C43S Non-standard monomer：Yes (specific site not provided by mmCIF) UAV (2R,3R)-2-hydroxy-3-methyl-2-[(2E,7S)-3,7,11,15-tetramethylhexadec-2-en-1-yl]-2,3-dihydronaphthalene-1,4-dione × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 6.5；295 K；25% PEG400, 0.1 M ammonium citrate dibasic, 0.1 M MES, pH 6.5
Resolution 2.80 Å R-free 0.270
6WV6 Human VKOR with phenindione Deposited 2020-05-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–144（144 aa） Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Chain A 146–231（86 aa） Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 2 UAS Phenindione × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 6.5；295 K；34% PEG400, 80 mM lithium sulfate, 0.1 M MES, pH 6.5
Resolution 2.70 Å R-free 0.268
6WV7 Human VKOR with Chlorophacinone Deposited 2020-05-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–144（144 aa） Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Chain A 146–231（86 aa） Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) UAJ Chlorophacinone × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 6.5；295 K；33% PEG400, 5% DMSO, 0.1 M sodium acetate, 0.1 M MES, pH 6.5
Resolution 2.48 Å R-free 0.245
6WV7 Human VKOR with Chlorophacinone Deposited 2020-05-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–144（144 aa） Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Chain B 146–231（86 aa） Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) UAJ Chlorophacinone × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 6.5；295 K；33% PEG400, 5% DMSO, 0.1 M sodium acetate, 0.1 M MES, pH 6.5
Resolution 2.48 Å R-free 0.245
6WV8 Takifugu rubripes VKOR-like C138S mutant with vitamin K1 Deposited 2020-05-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 146–238（93 aa）
Mutation：C138S Non-standard monomer：Yes (specific site not provided by mmCIF) PQN PHYLLOQUINONE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 6；295 K；25% PEG 400, 300 mM ammonium fluoride, 0.1 M MES pH 6.0
Resolution 3.01 Å R-free 0.293
6WVB Takifugu rubripes VKOR-like with warfarin Deposited 2020-05-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 146–238（93 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SWF S-WARFARIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 5.5；295 K；23% PEG400, 0.1 M Sodium nitrate, 0.1 M MES pH 5.5
Resolution 2.87 Å R-free 0.286
6WVH Human VKOR with Brodifacoum Deposited 2020-05-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–144（144 aa） Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Chain A 146–231（86 aa） Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) UA7 Brodifacoum × 1 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 6.5；295 K；32% PEG400, 0.1 M ammonium acetate, 1% 1,2,3-heptanetriol, 0.1 M MES, pH 6.5
Resolution 1.99 Å R-free 0.215
6WVH Human VKOR with Brodifacoum Deposited 2020-05-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–144（144 aa） Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Chain B 146–231（86 aa） Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) UA7 Brodifacoum × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 6.5；295 K；32% PEG400, 0.1 M ammonium acetate, 1% 1,2,3-heptanetriol, 0.1 M MES, pH 6.5
Resolution 1.99 Å R-free 0.215
7A7M rsGreen0.7b in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；150 mM KBr 30 % PEG 2000 mme
Resolution 1.60 Å R-free 0.198
7A7N rsGreen0.7b in the green-off state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MMT pH 5.0 25 % PEG 1500
Resolution 1.20 Å R-free 0.170
7A7O rsGreen0.7-K206A in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；200 mM Ammonium acetate 100 mM Bis-tris pH 5.5 25 % PEG 3350
Resolution 1.80 Å R-free 0.241
7A7P rsGreen0.7-K206A partially in the green-off state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) NO3 NITRATE ION × 1 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；22 % PEG 4000 140 mM Mg(NO3)2
Resolution 1.48 Å R-free 0.197
7A7Q rsGreenF-K206A in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PEG DI(HYDROXYETHYL)ETHER × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；200 mM NaCl 100 mM MES pH 6.0 20 % PEG 6000
Resolution 2.00 Å R-free 0.218
7A7R rsGreenF-K206A in the green-off state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PEG DI(HYDROXYETHYL)ETHER × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；200 mM NaCl 100 mM MES pH 6.0 20 % PEG 6000
Resolution 2.35 Å R-free 0.243
7A7S rsGreenF in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MIB pH 4.0 25 % PEG 1500
Resolution 1.73 Å R-free 0.235
7A7T rsGreenF partially in the green-off state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MIB pH 4.0 25 % PEG 1500
Resolution 1.58 Å R-free 0.197
7A7U rsGreen1 in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；2.0 M (NH4)2SO4 200 mM NaCl 100 mM Na-cacodylate pH 6.5
Resolution 2.15 Å R-free 0.201
7A7V rsGreen1-K206A partially in the green-off state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) GOL GLYCEROL × 1 PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 5.5 200 mM (NH4)2SO4 16 % PEG 8000
Resolution 2.00 Å R-free 0.194
7A7W rsGreen0.7-F145M partially in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；200 mM NH4HCO2 20 % PEG 4000
Resolution 1.30 Å R-free 0.165
7A7X rsGreen0.7-F145M in the green-off state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MMT pH 8.0 25 % PEG 1500
Resolution 1.85 Å R-free 0.207
7A7Y rsGreen0.7-F145Q in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) NO3 NITRATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；200 mM KNO3 20 % PEG 3350
Resolution 0.97 Å R-free 0.142
7A7Z rsGreen0.7-K206A-E222G in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；200 mM (Li)2SO4 100 mM Tris pH 8.5 40 % PEG 400
Resolution 1.10 Å R-free 0.142
7A80 rsGreen0.7-K206A-E222V in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) NO3 NITRATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；26 % PEG 4000 500 mM Mg(NO3) 50 mM HEPES pH 7.5
Resolution 2.05 Å R-free 0.234
7A80 rsGreen0.7-K206A-E222V in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) NO3 NITRATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；26 % PEG 4000 500 mM Mg(NO3) 50 mM HEPES pH 7.5
Resolution 2.05 Å R-free 0.234
7A80 rsGreen0.7-K206A-E222V in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) NO3 NITRATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；26 % PEG 4000 500 mM Mg(NO3) 50 mM HEPES pH 7.5
Resolution 2.05 Å R-free 0.234
7A80 rsGreen0.7-K206A-E222V in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) NO3 NITRATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；26 % PEG 4000 500 mM Mg(NO3) 50 mM HEPES pH 7.5
Resolution 2.05 Å R-free 0.234
7A81 rsGreen0.7-K206A-F145A partially in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；200 mM NaCl 100 mM MES pH 6.0 20 % PEG 6000 4% 2,2,2-trifluoroethanol
Resolution 1.70 Å R-free 0.248
7A82 rsGreen0.7-K206A-F145A partially in the green-off state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；200 mM NaCl 100 mM MES pH 6.0 20 % PEG 6000 200 mM NDSB-211
Resolution 1.90 Å R-free 0.250
7A83 rsGreen0.7-K206A-F145H in the green-on-state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PGE TRIETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；125 mM MES pH 6.0 9 % PEG 4000
Resolution 1.73 Å R-free 0.192
7A84 rsGreen0.7-K206A-F145H partially in the green-off state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MES pH 6.0 14 % PEG 400 300 mM NGSB-195
Resolution 2.10 Å R-free 0.269
7A85 rsGreen0.7-K206A-F145L in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PGE TRIETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MES pH 6.5 12 % PEG 20 000 10 mM NaBr
Resolution 1.52 Å R-free 0.237
7A86 rsGreen0.7-K206A-F145L partially in the green-off state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MES pH 6.5 12 % PEG 20 000 4 % Pentaerythritol ethoxylate
Resolution 1.90 Å R-free 0.210
7A86 rsGreen0.7-K206A-F145L partially in the green-off state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PGE TRIETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MES pH 6.5 12 % PEG 20 000 4 % Pentaerythritol ethoxylate
Resolution 1.90 Å R-free 0.210
7A87 rsGreen0.7-K206A-F145M in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 6.5 20 % PEG 1500
Resolution 1.75 Å R-free 0.228
7A88 rsGreen0.7-K206A-F145M in the green-off state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PGE TRIETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MMT 25 % PEG 1500
Resolution 1.95 Å R-free 0.226
7A89 rsGreen0.7-K206A-F145Q in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；22 % PEG 4000 140 mM Mg(NO3)2
Resolution 2.50 Å R-free 0.234
7A89 rsGreen0.7-K206A-F145Q in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；22 % PEG 4000 140 mM Mg(NO3)2
Resolution 2.50 Å R-free 0.234
7A8A rsGreen0.7-K206A-F145Q partially in the green-off state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；200 mM NaCl 100 mM MES pH 6.0 20 % PEG 6000
Resolution 1.93 Å R-free 0.254
7A8B rsGreen0.7-K206A-F145S partially in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MES pH 6.0 14 % PEG 4000
Resolution 1.55 Å R-free 0.232
7A8C rsGreen0.7-K206A-F145S in the green-off state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PGE TRIETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MES pH 6.5 25 % PEG 4000 400 mM D-galactose
Resolution 2.13 Å R-free 0.253
7A8D rsGreen0.7-K206A-F165W partially in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；200 mM NH4F 20 % PEG 3350
Resolution 1.65 Å R-free 0.219
7A8E rsGreen0.7-K206A-F165W partially in the green-off state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM HEPES pH 7.5 25 % PEG 4000
Resolution 2.20 Å R-free 0.261
7A8F rsGreen0.7-K206A-F165L in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM HEPES pH 7.5 25 % PEG 4000
Resolution 2.27 Å R-free 0.257
7A8G rsGreen0.7-K206A-H148G in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；200 mM TMAO 100mM Tris pH 8.5 20 % Peg 2000 mme
Resolution 2.00 Å R-free 0.217
7A8G rsGreen0.7-K206A-H148G in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PGE TRIETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；200 mM TMAO 100mM Tris pH 8.5 20 % Peg 2000 mme
Resolution 2.00 Å R-free 0.217
7A8H rsGreen0.7-K206A-H148S in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 6.5 20 % PEG 5000 mme
Resolution 1.70 Å R-free 0.209
7A8H rsGreen0.7-K206A-H148S in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 6.5 20 % PEG 5000 mme
Resolution 1.70 Å R-free 0.209
7A8H rsGreen0.7-K206A-H148S in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 6.5 20 % PEG 5000 mme
Resolution 1.70 Å R-free 0.209
7A8H rsGreen0.7-K206A-H148S in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 6.5 20 % PEG 5000 mme
Resolution 1.70 Å R-free 0.209
7A8I rsGreen0.7-K206A-H148S partially in the green-off state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 6.5 20 % PEG 5000 mme
Resolution 2.00 Å R-free 0.228
7A8I rsGreen0.7-K206A-H148S partially in the green-off state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 6.5 20 % PEG 5000 mme
Resolution 2.00 Å R-free 0.228
7A8I rsGreen0.7-K206A-H148S partially in the green-off state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 6.5 20 % PEG 5000 mme
Resolution 2.00 Å R-free 0.228
7A8I rsGreen0.7-K206A-H148S partially in the green-off state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 6.5 20 % PEG 5000 mme
Resolution 2.00 Å R-free 0.228
7A8J rsGreen0.7-K206A-H148V in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MES pH 6.5 25 % PEG 550 mme 10 mM ZnSO4
Resolution 1.85 Å R-free 0.232
7A8K rsGreen0.7-K206A-H148V partially in the green-off state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；50 mM HEPES pH 7.5 12 % PEG 4000 1% tryptone
Resolution 2.25 Å R-free 0.263
7A8L rsGreen0.7-K206A-N205C in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MES pH 6.5 25 % PEG 3000
Resolution 1.75 Å R-free 0.225
7A8M rsGreen0.7-K206A-N205G in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MES pH 6.0 20 % PEG 2000 mme 10 mM BaCl2
Resolution 1.60 Å R-free 0.245
7A8N rsGreen0.7-K206A-N205L in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100 mM HEPES pH 7.5 25 % PEG 8000
Resolution 2.10 Å R-free 0.258
7A8O rsGreen0.7-K206A-N205S in the green-on state Deposited 2020-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；200 mM Maic acid pH 7.0 20 % PEG 3350
Resolution 1.60 Å R-free 0.201
7BWN Crystal Structure of a Designed Protein Heterocatenane Deposited 2020-04-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain F 1–238（238 aa）
Mutation：R2S,S30R,A72S,Q80R,A206V,M340E,L344K No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；298.15 K；Bis-Tris, (NH4)2SO4
Resolution 2.40 Å R-free 0.239
7BWN Crystal Structure of a Designed Protein Heterocatenane Deposited 2020-04-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–238（238 aa）
Mutation：R2S,S30R,A72S,Q80R,A206V,M340E,L344K No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；298.15 K；Bis-Tris, (NH4)2SO4
Resolution 2.40 Å R-free 0.239
7BWN Crystal Structure of a Designed Protein Heterocatenane Deposited 2020-04-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1–238（238 aa）
Mutation：R2S,S30R,A72S,Q80R,A206V,M340E,L344K No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；298.15 K；Bis-Tris, (NH4)2SO4
Resolution 2.40 Å R-free 0.239
7BWN Crystal Structure of a Designed Protein Heterocatenane Deposited 2020-04-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 1–238（238 aa）
Mutation：R2S,S30R,A72S,Q80R,A206V,M340E,L344K No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；298.15 K；Bis-Tris, (NH4)2SO4
Resolution 2.40 Å R-free 0.239
7BWN Crystal Structure of a Designed Protein Heterocatenane Deposited 2020-04-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain H 1–238（238 aa）
Mutation：R2S,S30R,A72S,Q80R,A206V,M340E,L344K No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；298.15 K；Bis-Tris, (NH4)2SO4
Resolution 2.40 Å R-free 0.239
7BWN Crystal Structure of a Designed Protein Heterocatenane Deposited 2020-04-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain J 1–238（238 aa）
Mutation：R2S,S30R,A72S,Q80R,A206V,M340E,L344K No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；298.15 K；Bis-Tris, (NH4)2SO4
Resolution 2.40 Å R-free 0.239
7BWN Crystal Structure of a Designed Protein Heterocatenane Deposited 2020-04-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 7 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain M 1–238（238 aa）
Mutation：R2S,S30R,A72S,Q80R,A206V,M340E,L344K No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；298.15 K；Bis-Tris, (NH4)2SO4
Resolution 2.40 Å R-free 0.239
7BWN Crystal Structure of a Designed Protein Heterocatenane Deposited 2020-04-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 8 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain O 1–238（238 aa）
Mutation：R2S,S30R,A72S,Q80R,A206V,M340E,L344K No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；298.15 K；Bis-Tris, (NH4)2SO4
Resolution 2.40 Å R-free 0.239
7E53 Crystal structure of sfGFP complexed with the nanobody nb2 at 2.2 Angstron resolution Deposited 2021-02-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 3–232（230 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；30% (w/v) Polyethylene glycol 400, 100mM CAPS/ Sodium hydroxide, pH 10.5
Resolution 2.21 Å R-free 0.258
8DHY N-terminal fragment of MsbA fused to GFP in complex with copper(II) Deposited 2022-06-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–239（237 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) CU COPPER (II) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；70% Tacsimate pH 7.0
Resolution 2.15 Å R-free 0.228
8GW6 AtSLAC1 6D mutant in closed state Deposited 2022-09-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 1–240（240 aa）
Chain B 1–240（240 aa）
Chain C 1–240（240 aa）
Mutation：T62D,S65D,S107D,S124D,S146D,T152D Mutation：T62D,S65D,S107D,S124D,S146D,T152D Mutation：T62D,S65D,S107D,S124D,S146D,T152D CL CHLORIDE ION × 3 Y01 CHOLESTEROL HEMISUCCINATE × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8GW7 AtSLAC1 6D mutant in open state Deposited 2022-09-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 1–240（240 aa）
Chain B 1–240（240 aa）
Chain C 1–240（240 aa）
Mutation：T62D,S65D,S107D,S124D,S146D,T152D Mutation：T62D,S65D,S107D,S124D,S146D,T152D Mutation：T62D,S65D,S107D,S124D,S146D,T152D CL CHLORIDE ION × 6 Y01 CHOLESTEROL HEMISUCCINATE × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8J6O transport T2 Deposited 2023-04-26 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 3–236（234 aa）
Chain B 3–236（234 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.25 Å
8J7M ion channel Deposited 2023-04-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 3–235（233 aa）
Chain B 3–235（233 aa）
Chain C 3–235（233 aa）
Chain D 3–235（233 aa）
Not recorded POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 CLR CHOLESTEROL × 10 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8RL9 RECQL5:sfGFP hetero dimer assembled by Di-Gluebody Deposited 2024-01-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–238（238 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.22 Å
8RLB RECQL5:sfGFP hetero dimer assembled by Di-Gluebody - sfGFP local refinement Deposited 2024-01-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–238（238 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.99 Å
8RLC SPNS2:sfGFP hetero dimer assembled by Di-Gluebody Deposited 2024-01-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–238（238 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8RLE SPNS2:sfGFP hetero dimer assembled by Di-Gluebody - sfGFP local refinement Deposited 2024-01-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–238（238 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.75 Å
8UBG DpHF19 filament Deposited 2023-09-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 20 PDB declaration: 20-meric
Chain A 3–238（236 aa）
Chain B 3–238（236 aa）
Chain C 3–238（236 aa）
Chain D 3–238（236 aa）
Chain E 3–238（236 aa）
Chain F 3–238（236 aa）
Chain G 3–238（236 aa）
Chain H 3–238（236 aa）
Chain I 3–238（236 aa）
Chain J 3–238（236 aa）
Chain K 3–238（236 aa）
Chain L 3–238（236 aa）
Chain M 3–238（236 aa）
Chain N 3–238（236 aa）
Chain O 3–238（236 aa）
Chain P 3–238（236 aa）
Chain Q 3–238（236 aa）
Chain R 3–238（236 aa）
Chain S 3–238（236 aa）
Chain T 3–238（236 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XAL Cryo-EM structure of SARS-CoV-2 S-BQ.1 in complex with ACE2 Deposited 2023-12-04 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric
Chain I 3–238（236 aa）
Chain J 3–238（236 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 31 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
Resolution 3.20 Å
9BCF Chimeric protein of crocodile allergen Cro p 1.0101 and GFP Deposited 2024-04-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；0.2 M calcium acetate, 0.1 M sodium cacodylate pH 6.5, 40% v/v PEG 300
Resolution 3.20 Å R-free 0.238
9BCF Chimeric protein of crocodile allergen Cro p 1.0101 and GFP Deposited 2024-04-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain B 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；0.2 M calcium acetate, 0.1 M sodium cacodylate pH 6.5, 40% v/v PEG 300
Resolution 3.20 Å R-free 0.238
9BCF Chimeric protein of crocodile allergen Cro p 1.0101 and GFP Deposited 2024-04-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain C 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；0.2 M calcium acetate, 0.1 M sodium cacodylate pH 6.5, 40% v/v PEG 300
Resolution 3.20 Å R-free 0.238
9C74 superfolder Green Fluorescent Protein with meta-nitro-tyrosine incorporated at position 66 Deposited 2024-06-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–238（238 aa）
Mutation：Y66mNO2Y Non-standard monomer：Yes (specific site not provided by mmCIF) NA SODIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；1% tryptone, 0.001 M NaN3, 0.05 M Hepes pH 7.0, 20% w/v PEG350
Resolution 1.51 Å R-free 0.220
9IRV MultiBody Refinement of dimeric DARPin and its bound GFP on a symmetric scaffold Deposited 2024-07-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1–231（231 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.47 Å
9J48 GFP bound to 24-mer DARPin-apoferritin model 6c Deposited 2024-08-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric
Chain a 1–231（231 aa）
Chain b 1–231（231 aa）
Chain c 1–231（231 aa）
Chain d 1–231（231 aa）
Chain e 1–231（231 aa）
Chain f 1–231（231 aa）
Chain g 1–231（231 aa）
Chain h 1–231（231 aa）
Chain i 1–231（231 aa）
Chain j 1–231（231 aa）
Chain k 1–231（231 aa）
Chain l 1–231（231 aa）
Chain m 1–231（231 aa）
Chain n 1–231（231 aa）
Chain o 1–231（231 aa）
Chain p 1–231（231 aa）
Chain q 1–231（231 aa）
Chain r 1–231（231 aa）
Chain s 1–231（231 aa）
Chain t 1–231（231 aa）
Chain v 1–231（231 aa）
Chain w 1–231（231 aa）
Chain x 1–231（231 aa）
Chain y 1–231（231 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
9N6A GP23 C-termimal receptor binding domain from TM4 phage Deposited 2025-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 1–238（238 aa）
Not recorded SO4 SULFATE ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；289 K；0.2 M Lithium Sulfate 0.1 M MES: NaOH, pH 6 20 % (v/v) 1,4-Butanediol
Resolution 2.91 Å R-free 0.280
9V85 Superfolder GFP fused gp38 receptor binding domain of bacteriophage PP01 Deposited 2025-05-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293.15 K；10% (w/v) PEG8000, 0.1M Na-HEPES-MOPS, pH7.5, 3mM CaCl2, 3mM MgCl2, 20 % (v/v) ethylene glycol
Resolution 2.10 Å R-free 0.208
9V85 Superfolder GFP fused gp38 receptor binding domain of bacteriophage PP01 Deposited 2025-05-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain C 3–238（236 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293.15 K；10% (w/v) PEG8000, 0.1M Na-HEPES-MOPS, pH7.5, 3mM CaCl2, 3mM MgCl2, 20 % (v/v) ethylene glycol
Resolution 2.10 Å R-free 0.208
9ZG5 Structure of superfolder GFP bound to nanobody 15 Deposited 2025-12-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 3–239（237 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.40 Å R-free 0.331
9ZG5 Structure of superfolder GFP bound to nanobody 15 Deposited 2025-12-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 3–239（237 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.40 Å R-free 0.331
9ZG5 Structure of superfolder GFP bound to nanobody 15 Deposited 2025-12-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 3–239（237 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.40 Å R-free 0.331
9ZG5 Structure of superfolder GFP bound to nanobody 15 Deposited 2025-12-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 3–239（237 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.40 Å R-free 0.331

91 other PDB entries and 137 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name A0A059PIQ0_AEQVI
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 50–283； UniProt 3–236 Author chain B； PDBConstruct 50–283； UniProt 3–236

Green fluorescent protein,SID1 transmembrane family member 1

Homo sapiens

UniProt Q9NXL6

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Insufficient information Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 20–827 Chain B； UniProt 20–827 Not recorded CLR CHOLESTEROL × 8 ZN ZINC ION × 2 NA SODIUM ION × 1 OLA OLEIC ACID × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.77 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8J6M Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
8JUL Cryo-EM structure of SIDT1 in complex with phosphatidic acid Deposited 2023-06-26 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–827（827 aa）
Chain B 1–827（827 aa）
Not recorded ZN ZINC ION × 2 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.92 Å
8JUN Cryo-EM structure of SIDT1 E555Q mutant Deposited 2023-06-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–827（827 aa）
Chain B 1–827（827 aa）
Mutation：E555Q Mutation：E555Q ZN ZINC ION × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.38 Å
8K13 SID1 transmembrane family member 1 Deposited 2023-07-10 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 21–827（807 aa）
Chain B 21–827（807 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.33 Å
8K1B SID1 transmembrane family member 1 Deposited 2023-07-10 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 47–300（254 aa）
Chain B 47–300（254 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.47 Å
8K1D SID1 transmembrane family member 1 Deposited 2023-07-10 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 301–827（527 aa）
Chain B 301–827（527 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.53 Å
8KCW Cryo-EM structure of human SIDT1 bound to cholesterol Deposited 2023-08-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–827（827 aa）
Chain C 1–827（827 aa）
Not recorded ZN ZINC ION × 2 CLR CHOLESTEROL × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.77 Å
8KCX Cryo-EM structure of human SIDT1 Deposited 2023-08-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–827（827 aa）
Chain C 1–827（827 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.96 Å
8V38 Structure of the human systemic RNAi defective transmembrane protein 1 (hSIDT1) Deposited 2023-11-27 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–827（827 aa）
Chain B 1–827（827 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8WOQ Cryo-EM structure of human SIDT1 protein with C1 symmetry at neutral pH Deposited 2023-10-07 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–832（832 aa）
Chain B 1–832（832 aa）
Not recorded ZN ZINC ION × 2 CA CALCIUM ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CLR CHOLESTEROL × 15 OLA OLEIC ACID × 2 SPL OCTANOIC ACID (2-HYDROXY-1-HYDROXYMETHYL-HEPTADEC-3-ENYL)-AMIDE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.85 Å
8WOR Cryo-EM structure of human SIDT1 protein with C2 symmetry at neutral pH Deposited 2023-10-07 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–832（832 aa）
Chain B 1–832（832 aa）
Not recorded ZN ZINC ION × 2 CA CALCIUM ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CLR CHOLESTEROL × 15 OLA OLEIC ACID × 2 SPL OCTANOIC ACID (2-HYDROXY-1-HYDROXYMETHYL-HEPTADEC-3-ENYL)-AMIDE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å
8WOS Cryo-EM structure of human SIDT1 protein with C1 symmetry at low pH Deposited 2023-10-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–832（832 aa）
Chain B 1–832（832 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CLR CHOLESTEROL × 8 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 5.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.37 Å
8WOT Cryo-EM structure of human SIDT1 protein with C2 symmetry at low pH Deposited 2023-10-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–832（832 aa）
Chain B 1–832（832 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CLR CHOLESTEROL × 8 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 5.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.18 Å
9M8U Cryo-EM structure of human SIDT1 Deposited 2025-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–832（832 aa）
Chain B 1–832（832 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.79 Å

13 other PDB entries and 13 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name SIDT1_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 291–1098； UniProt 20–827 Author chain B； PDBConstruct 291–1098； UniProt 20–827

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 8j6m

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 8j6m
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2. Structure Basics 2. Structure Basics

Entry ID entry_id8j6m
Deposition date deposition_date2023-04-26
Structure title titleSIDT1 protein
Keywords keywordstransport T1, TRANSCRIPTION; TRANSCRIPTION
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier37.35 Å
Radius of gyration Rg (electron density) rg_electron36.90 Å
Forward intensity I(0) i0271005000.00
Molecular weight molecular_weight149630.0 kDa
Excluded volume excluded_volume193580 ų
Envelope volume envelope_volume237120 ų
Hydration-shell volume shell_volume53121 ų
Envelope diameter envelope_diameter124.3 Å
Shell Rg shell_rg43.00 Å
Envelope Rg envelope_rg37.08 Å
Shape Rg shape_rg36.89 Å
Total Rg total_rg37.36 Å
Total atoms total_atoms10579
Residues n_residues1282
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax127.0 Å
Rg (real space) rg_real37.41 Å
Rg uncertainty (real space) rg_real_error1.40 Å
I(0) (real space) i0_real2.7100e+08
I(0) uncertainty (real space) i0_real_error4.9960e+06
Rg (reciprocal space) rg_reciprocal37.37 Å
I(0) (reciprocal space) i0_reciprocal271000000.0000
Solution quality estimate total_estimate0.8824
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary42.3 Å
Skewness Skewness skewness0.380
Kurtosis Kurtosis kurtosis-0.375
Angular range angular_range— – 0.2100 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha55070000.0000
Real-space data points n_real_points43
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.847; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.992; Smooth: 0.934
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (6)

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8. Citations (1)

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9. Files and Curves (10)