8r2i

Cryo-EM Structure of native Photosystem II assembly intermediate from Chlamydomonas reinhardtii

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Photosystem II protein D1

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 11	Photosystem II CP47 reaction center protein × 1 （P37255） Photosystem II CP43 reaction center protein × 1 （P10898） Photosystem II D2 protein × 1 （P06007） Cytochrome b559 subunit alpha × 1 （P48268） Cytochrome b559 subunit beta × 1 （Q08363） Photosystem II reaction center protein H × 1 （P22666） Photosystem II reaction center protein I × 1 （P59763） Photosystem II reaction center protein K × 1 （P18263） Chain U, Predicted protein × 1 （A8I5A0） Photosystem II reaction center protein Ycf12 × 1 （P50370） FE (II) ION × 1 CHLOROPHYLL A × 35 PHEOPHYTIN A × 2 BETA-CAROTENE × 8 octyl beta-D-glucopyranoside × 3 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 DODECYL-BETA-D-MALTOSIDE × 2 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 1 BICARBONATE ION × 1 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 1 PROTOPORPHYRIN IX CONTAINING FE × 1 water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBA_CHLRE
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–327； UniProt 11–337

Photosystem II CP47 reaction center protein

OrganismNot specified

UniProt P37255

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 11	Photosystem II protein D1 × 1 （P07753） Photosystem II CP43 reaction center protein × 1 （P10898） Photosystem II D2 protein × 1 （P06007） Cytochrome b559 subunit alpha × 1 （P48268） Cytochrome b559 subunit beta × 1 （Q08363） Photosystem II reaction center protein H × 1 （P22666） Photosystem II reaction center protein I × 1 （P59763） Photosystem II reaction center protein K × 1 （P18263） Chain U, Predicted protein × 1 （A8I5A0） Photosystem II reaction center protein Ycf12 × 1 （P50370） FE (II) ION × 1 CHLOROPHYLL A × 35 PHEOPHYTIN A × 2 BETA-CAROTENE × 8 octyl beta-D-glucopyranoside × 3 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 DODECYL-BETA-D-MALTOSIDE × 2 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 1 BICARBONATE ION × 1 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 1 PROTOPORPHYRIN IX CONTAINING FE × 1 water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBB_CHLRE
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–487； UniProt 2–488

Photosystem II CP43 reaction center protein

OrganismNot specified

UniProt P10898

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 11	Photosystem II protein D1 × 1 （P07753） Photosystem II CP47 reaction center protein × 1 （P37255） Photosystem II D2 protein × 1 （P06007） Cytochrome b559 subunit alpha × 1 （P48268） Cytochrome b559 subunit beta × 1 （Q08363） Photosystem II reaction center protein H × 1 （P22666） Photosystem II reaction center protein I × 1 （P59763） Photosystem II reaction center protein K × 1 （P18263） Chain U, Predicted protein × 1 （A8I5A0） Photosystem II reaction center protein Ycf12 × 1 （P50370） FE (II) ION × 1 CHLOROPHYLL A × 35 PHEOPHYTIN A × 2 BETA-CAROTENE × 8 octyl beta-D-glucopyranoside × 3 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 DODECYL-BETA-D-MALTOSIDE × 2 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 1 BICARBONATE ION × 1 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 1 PROTOPORPHYRIN IX CONTAINING FE × 1 water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBC_CHLRE
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 1–423； UniProt 21–443

Photosystem II D2 protein

OrganismNot specified

UniProt P06007

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 11	Photosystem II protein D1 × 1 （P07753） Photosystem II CP47 reaction center protein × 1 （P37255） Photosystem II CP43 reaction center protein × 1 （P10898） Cytochrome b559 subunit alpha × 1 （P48268） Cytochrome b559 subunit beta × 1 （Q08363） Photosystem II reaction center protein H × 1 （P22666） Photosystem II reaction center protein I × 1 （P59763） Photosystem II reaction center protein K × 1 （P18263） Chain U, Predicted protein × 1 （A8I5A0） Photosystem II reaction center protein Ycf12 × 1 （P50370） FE (II) ION × 1 CHLOROPHYLL A × 35 PHEOPHYTIN A × 2 BETA-CAROTENE × 8 octyl beta-D-glucopyranoside × 3 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 DODECYL-BETA-D-MALTOSIDE × 2 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 1 BICARBONATE ION × 1 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 1 PROTOPORPHYRIN IX CONTAINING FE × 1 water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBD_CHLRE
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain D； PDBConstruct 1–350； UniProt 3–352

Cytochrome b559 subunit alpha

OrganismNot specified

UniProt P48268

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 11	Photosystem II protein D1 × 1 （P07753） Photosystem II CP47 reaction center protein × 1 （P37255） Photosystem II CP43 reaction center protein × 1 （P10898） Photosystem II D2 protein × 1 （P06007） Cytochrome b559 subunit beta × 1 （Q08363） Photosystem II reaction center protein H × 1 （P22666） Photosystem II reaction center protein I × 1 （P59763） Photosystem II reaction center protein K × 1 （P18263） Chain U, Predicted protein × 1 （A8I5A0） Photosystem II reaction center protein Ycf12 × 1 （P50370） FE (II) ION × 1 CHLOROPHYLL A × 35 PHEOPHYTIN A × 2 BETA-CAROTENE × 8 octyl beta-D-glucopyranoside × 3 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 DODECYL-BETA-D-MALTOSIDE × 2 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 1 BICARBONATE ION × 1 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 1 PROTOPORPHYRIN IX CONTAINING FE × 1 water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBE_CHLRE
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain E； PDBConstruct 1–76； UniProt 7–82

Cytochrome b559 subunit beta

OrganismNot specified

UniProt Q08363

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 11	Photosystem II protein D1 × 1 （P07753） Photosystem II CP47 reaction center protein × 1 （P37255） Photosystem II CP43 reaction center protein × 1 （P10898） Photosystem II D2 protein × 1 （P06007） Cytochrome b559 subunit alpha × 1 （P48268） Photosystem II reaction center protein H × 1 （P22666） Photosystem II reaction center protein I × 1 （P59763） Photosystem II reaction center protein K × 1 （P18263） Chain U, Predicted protein × 1 （A8I5A0） Photosystem II reaction center protein Ycf12 × 1 （P50370） FE (II) ION × 1 CHLOROPHYLL A × 35 PHEOPHYTIN A × 2 BETA-CAROTENE × 8 octyl beta-D-glucopyranoside × 3 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 DODECYL-BETA-D-MALTOSIDE × 2 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 1 BICARBONATE ION × 1 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 1 PROTOPORPHYRIN IX CONTAINING FE × 1 water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBF_CHLRE
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain F； PDBConstruct 1–33； UniProt 12–44

Photosystem II reaction center protein H

OrganismNot specified

UniProt P22666

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 11	Photosystem II protein D1 × 1 （P07753） Photosystem II CP47 reaction center protein × 1 （P37255） Photosystem II CP43 reaction center protein × 1 （P10898） Photosystem II D2 protein × 1 （P06007） Cytochrome b559 subunit alpha × 1 （P48268） Cytochrome b559 subunit beta × 1 （Q08363） Photosystem II reaction center protein I × 1 （P59763） Photosystem II reaction center protein K × 1 （P18263） Chain U, Predicted protein × 1 （A8I5A0） Photosystem II reaction center protein Ycf12 × 1 （P50370） FE (II) ION × 1 CHLOROPHYLL A × 35 PHEOPHYTIN A × 2 BETA-CAROTENE × 8 octyl beta-D-glucopyranoside × 3 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 DODECYL-BETA-D-MALTOSIDE × 2 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 1 BICARBONATE ION × 1 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 1 PROTOPORPHYRIN IX CONTAINING FE × 1 water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBH_CHLRE
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain H； PDBConstruct 1–65； UniProt 19–83

Photosystem II reaction center protein I

OrganismNot specified

UniProt P59763

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 11	Photosystem II protein D1 × 1 （P07753） Photosystem II CP47 reaction center protein × 1 （P37255） Photosystem II CP43 reaction center protein × 1 （P10898） Photosystem II D2 protein × 1 （P06007） Cytochrome b559 subunit alpha × 1 （P48268） Cytochrome b559 subunit beta × 1 （Q08363） Photosystem II reaction center protein H × 1 （P22666） Photosystem II reaction center protein K × 1 （P18263） Chain U, Predicted protein × 1 （A8I5A0） Photosystem II reaction center protein Ycf12 × 1 （P50370） FE (II) ION × 1 CHLOROPHYLL A × 35 PHEOPHYTIN A × 2 BETA-CAROTENE × 8 octyl beta-D-glucopyranoside × 3 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 DODECYL-BETA-D-MALTOSIDE × 2 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 1 BICARBONATE ION × 1 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 1 PROTOPORPHYRIN IX CONTAINING FE × 1 water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBI_CHLRE
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain I； PDBConstruct 1–28； UniProt 1–28

Photosystem II reaction center protein K

OrganismNot specified

UniProt P18263

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 11	Photosystem II protein D1 × 1 （P07753） Photosystem II CP47 reaction center protein × 1 （P37255） Photosystem II CP43 reaction center protein × 1 （P10898） Photosystem II D2 protein × 1 （P06007） Cytochrome b559 subunit alpha × 1 （P48268） Cytochrome b559 subunit beta × 1 （Q08363） Photosystem II reaction center protein H × 1 （P22666） Photosystem II reaction center protein I × 1 （P59763） Chain U, Predicted protein × 1 （A8I5A0） Photosystem II reaction center protein Ycf12 × 1 （P50370） FE (II) ION × 1 CHLOROPHYLL A × 35 PHEOPHYTIN A × 2 BETA-CAROTENE × 8 octyl beta-D-glucopyranoside × 3 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 DODECYL-BETA-D-MALTOSIDE × 2 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 1 BICARBONATE ION × 1 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 1 PROTOPORPHYRIN IX CONTAINING FE × 1 water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBK_CHLRE
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain K； PDBConstruct 1–37； UniProt 10–46

Chain U, Predicted protein

OrganismNot specified

UniProt A8I5A0

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 11	Photosystem II protein D1 × 1 （P07753） Photosystem II CP47 reaction center protein × 1 （P37255） Photosystem II CP43 reaction center protein × 1 （P10898） Photosystem II D2 protein × 1 （P06007） Cytochrome b559 subunit alpha × 1 （P48268） Cytochrome b559 subunit beta × 1 （Q08363） Photosystem II reaction center protein H × 1 （P22666） Photosystem II reaction center protein I × 1 （P59763） Photosystem II reaction center protein K × 1 （P18263） Photosystem II reaction center protein Ycf12 × 1 （P50370） FE (II) ION × 1 CHLOROPHYLL A × 35 PHEOPHYTIN A × 2 BETA-CAROTENE × 8 octyl beta-D-glucopyranoside × 3 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 DODECYL-BETA-D-MALTOSIDE × 2 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 1 BICARBONATE ION × 1 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 1 PROTOPORPHYRIN IX CONTAINING FE × 1 water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A8I5A0_CHLRE
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain 2； PDBConstruct 1–101； UniProt 75–175

Photosystem II reaction center protein Ycf12

OrganismNot specified

UniProt P50370

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 11	Photosystem II protein D1 × 1 （P07753） Photosystem II CP47 reaction center protein × 1 （P37255） Photosystem II CP43 reaction center protein × 1 （P10898） Photosystem II D2 protein × 1 （P06007） Cytochrome b559 subunit alpha × 1 （P48268） Cytochrome b559 subunit beta × 1 （Q08363） Photosystem II reaction center protein H × 1 （P22666） Photosystem II reaction center protein I × 1 （P59763） Photosystem II reaction center protein K × 1 （P18263） Chain U, Predicted protein × 1 （A8I5A0） FE (II) ION × 1 CHLOROPHYLL A × 35 PHEOPHYTIN A × 2 BETA-CAROTENE × 8 octyl beta-D-glucopyranoside × 3 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 DODECYL-BETA-D-MALTOSIDE × 2 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 1 BICARBONATE ION × 1 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 1 PROTOPORPHYRIN IX CONTAINING FE × 1 water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	YCF12_CHLRE
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain 1； PDBConstruct 1–32； UniProt 1–32

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	8r2i
Deposition date deposition_date	2023-11-06
Structure title title	Cryo-EM Structure of native Photosystem II assembly intermediate from Chlamydomonas reinhardtii
Keywords keywords	Photosystem II, photosynthesis, biogenesis of PSII, assembly intermediate, CryoEM, assembly factor, Chlamydomonas reinhardtii; PHOTOSYNTHESIS
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8r2i__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8r2i__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

8r2i__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	41.45 Å
Rg (electron density)	40.77 Å
Total Rg	41.25 Å
Atom count	18049
Residues	1923
Excluded volume	332740 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`8r2i__assembly_1__model_1`	undecameric （11）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (24)

Entity 1 Entity 1 polymer

Description description	Photosystem II protein D1
Molecular weight formula_weight	36287.3 kDa
EC 编号 ec_number	1.10.3.9
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	18054

SSLWARFCEWITSTENRLYIGWFGVIMIPCLLTATSVFIIAFIAAPPVDIDGIREPVSGSLLYGNNIITGAVIPTSNAIGLHFYPIWEAASLDEWLYNGGPYQLIVCHFLLGVYCYMGREWELSFRLGMRPWIAVAYSAPVAAASAVFLVYPIGQGSFSDGMPLGISGTFNFMIVFQAEHNILMHPFHMLGVAGVFGGSLFSAMHGSLVTSSLIRETTENESANEGYRFGQEEETYNIVAAHGYFGRLIFQYASFNNSRSLHFFLAAWPVIGIWFTALGLSTMAFNLNGFNFNQSVVDSQGRVLNTWADIINRANLGMEVMHERNAH

Entity 2 Entity 2 polymer

Description description	Photosystem II CP47 reaction center protein
Molecular weight formula_weight	53739.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

GLPWYRVHTVVINDPGRLISVHLMHTALVSGWAGSMALFEISVFDPSDPVLNPMWRQGMFVLPFMTRLGITQSWGGWTISGETATNPGIWSYEGVAAAHIILSGALFLASVWHWTYWDLELFRDPRTGKTALDLPKIFGIHLFLSGLLCFGFGAFHVTGVFGPGIWVSDPYGLTGRVQPVAPSWGADGFDPYNPGGIASHHIAAGILGVLAGLFHLCVRPSIRLYFGLSMGSIETVLSSSIAAVFWAAFVVAGTMWYGSAATPIELFGPTRYQWDQGFFQQEIQKRVQASLAEGASLSDAWSRIPEKLAFYDYIGNNPAKGGLFRTGAMNSGDGIAVGWLGHASFKDQEGRELFVRRMPTFFETFPVLLLDKDGIVRADVPFRKAESKYSIEQVGVSVTFYGGELDGLTFTDPATVKKYARKAQLGEIFEFDRSTLQSDGVFRSSPRGWFTFGHVCFALLFFFGHIWHGARTIFRDVFAGIDDDIND

Entity 3 Entity 3 polymer

Description description	Photosystem II CP43 reaction center protein
Molecular weight formula_weight	46468.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C

FAWWSGNARLINLSGKLLGAHVAHAGLIVFWAGAMNLFEVSHFVPEKPMYEQGLILLPHIATLGYGVGPGGEIIDTFPYFVSGVLHLISSAVLGFGGVYHSLIGPETLEESYPFFGYVWKDKNKMTNILGYHLIMLGLGAWLLVWKAMYFGGVYDTWAPGGGDVRVITNPTTNAAVIFGYLVKSPFGGDGWICSVDNMEDIIGGHIWIGTLEILGGIWHIYTTPWPWARRAFVWSGEAYLSYSLGAIGVMGFIACCMSWFNNTAYPSEFYGPTGPEASQSQAFTFLVRDQRLGANVASAQGPTGLGKYLMRSPTGEIIFGGETMRFWDFRGPWLEPLRGPNGLDLNKLKNDIQPWQERRAAEYMTHAPLGSLNSVGGVATEINAVNFVSPRSWLACSHFCLGFFFFIGHLWHAGRARAAAAGF

Entity 4 Entity 4 polymer

Description description	Photosystem II D2 protein
Molecular weight formula_weight	39241.7 kDa
EC 编号 ec_number	1.10.3.9
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D

IAIGTYQEKRTWFDDADDWLRQDRFVFVGWSGLLLFPCAYFALGGWLTGTTFVTSWYTHGLATSYLEGCNFLTAAVSTPANSMAHSLLFVWGPEAQGDFTRWCQLGGLWAFVALHGAFGLIGFMLRQFEIARSVNLRPYNAIAFSAPIAVFVSVFLIYPLGQSGWFFAPSFGVAAIFRFILFFQGFHNWTLNPFHMMGVAGVLGAALLCAIHGATVENTLFEDGDGANTFRAFNPTQAEETYSMVTANRFWSQIFGVAFSNKRWLHFFMLLVPVTGLWMSAIGVVGLALNLRAYDFVSQEIRAAEDPEFETFYTKNILLNEGIRAWMAAQDQPHERLVFPEEVLPRGNAL

Entity 5 Entity 5 polymer

Description description	Cytochrome b559 subunit alpha
Molecular weight formula_weight	8727.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

ERPFSDILTSIRYWVIHSITVPALFIAGWLFVSTGLAYDVFGTPRPNEYFTEDRQEAPLITDRFNALEQVKKLSGN

Entity 6 Entity 6 polymer

Description description	Cytochrome b559 subunit beta
Molecular weight formula_weight	3795.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

YPIFTVRWLAIHGIAVPTIFFLGAITAMQFIQR

Entity 7 Entity 7 polymer

Description description	Photosystem II reaction center protein H
Molecular weight formula_weight	7063.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H

EPGLVTPLGTLLRPLNSEAGKVLPGWGTTVLMAVFILLFAAFLLIILEIYNSSLILDDVSMSWET

Entity 8 Entity 8 polymer

Description description	Photosystem II reaction center protein I
Molecular weight formula_weight	3277.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I

MLTLKIFVYTVVTFFVCLFIFGFLSNDP

Entity 9 Entity 9 polymer

Description description	Photosystem II reaction center protein K
Molecular weight formula_weight	4148.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K

KLPEAYAPFAPIVDVLPVIPVFFILLAFVWQAAVSFR

Entity 10 Entity 10 polymer

Description description	Chain U, Predicted protein
Molecular weight formula_weight	11213.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	2

FDTYTAETSAILDKVKVTLALDKDDPAKEDSVKGLRKDINNWVAKYRREPKVSGKPSFGNTYSALNALAGHFNSFGATAPIPKKRLERLQKELDDATLLLT

Entity 11 Entity 11 polymer

Description description	Photosystem II reaction center protein Ycf12
Molecular weight formula_weight	3219.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	1

MNIELALTLVSLVLVVSAGPLVVVLLSARGGN

Entity 12 Entity 12 non-polymer

Description description	FE (II) ION
Molecular weight formula_weight	55.8 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 13 Entity 13 non-polymer

Description description	CHLOROPHYLL A
Molecular weight formula_weight	893.5 kDa
Source method src_method	syn
Copy count entity_count	35

Entity 14 Entity 14 non-polymer

Description description	PHEOPHYTIN A
Molecular weight formula_weight	871.2 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 15 Entity 15 non-polymer

Description description	BETA-CAROTENE
Molecular weight formula_weight	536.9 kDa
Source method src_method	syn
Copy count entity_count	8

Entity 16 Entity 16 non-polymer

Description description	octyl beta-D-glucopyranoside
Molecular weight formula_weight	292.4 kDa
Source method src_method	syn
Copy count entity_count	3

Entity 17 Entity 17 non-polymer

Description description	1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
Molecular weight formula_weight	787.2 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 18 Entity 18 non-polymer

Description description	DODECYL-BETA-D-MALTOSIDE
Molecular weight formula_weight	510.6 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 19 Entity 19 non-polymer

Description description	1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
Molecular weight formula_weight	723.0 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 20 Entity 20 non-polymer

Description description	DIGALACTOSYL DIACYL GLYCEROL (DGDG)
Molecular weight formula_weight	949.3 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 21 Entity 21 non-polymer

Description description	BICARBONATE ION
Molecular weight formula_weight	61.0 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 22 Entity 22 non-polymer

Description description	;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ;
Molecular weight formula_weight	749.2 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 23 Entity 23 non-polymer

Description description	PROTOPORPHYRIN IX CONTAINING FE
Molecular weight formula_weight	616.5 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 24 Entity 24 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	5

▶

Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	triclinic

Resolution resolution	2.90 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	327737

Cryo-EM Structure of native Photosystem II assembly intermediate from Chlamydomonas reinhardtii

1. Protein Identity and Related Structures Protein Identity & Related Structures

Photosystem II protein D1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Photosystem II CP47 reaction center protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Photosystem II CP43 reaction center protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Photosystem II D2 protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Cytochrome b559 subunit alpha

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Cytochrome b559 subunit beta

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Photosystem II reaction center protein H

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Photosystem II reaction center protein I

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Photosystem II reaction center protein K

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Chain U, Predicted protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer