9b8p

Synaptic Vesicle V-ATPase with synaptophysin and SidK, State 3, V1

Method: ELECTRON MICROSCOPY Dmax: 171.2 Å Quality: GOOD

1. Protein Identity and Related Structures Protein Identity & Related Structures

H(+)-transporting two-sector ATPase

OrganismNot specified

UniProt D4A133

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 17 PDB declaration: heptadecameric(17) Consistent with protein copy count Chain A; UniProt 1–647 Chain B; UniProt 1–647 Chain C; UniProt 1–647 Not recorded V-type proton ATPase subunit B, brain isoform × 3 (P62815) ATPase H+-transporting V1 subunit D × 1 (Q6P503) V-type proton ATPase subunit E 1 × 3 (Q6PCU2) V-type proton ATPase subunit F × 1 (P50408) V-type proton ATPase subunit G × 3 (Q8R2H0) SidK × 3 (Q5ZWW6) ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.4 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 3.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

15 other PDB entries and 15 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name D4A133_RAT
Isoform
PDB entities 1
Chains and sequence ranges Author chain A; PDBConstruct 1–647; UniProt 1–647 Author chain B; PDBConstruct 1–647; UniProt 1–647 Author chain C; PDBConstruct 1–647; UniProt 1–647

V-type proton ATPase subunit B, brain isoform

OrganismNot specified

UniProt P62815

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 17 PDB declaration: heptadecameric(17) Consistent with protein copy count Chain D; UniProt 1–511 Chain E; UniProt 1–511 Chain F; UniProt 1–511 Not recorded H(+)-transporting two-sector ATPase × 3 (D4A133) ATPase H+-transporting V1 subunit D × 1 (Q6P503) V-type proton ATPase subunit E 1 × 3 (Q6PCU2) V-type proton ATPase subunit F × 1 (P50408) V-type proton ATPase subunit G × 3 (Q8R2H0) SidK × 3 (Q5ZWW6) ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.4 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 3.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

15 other PDB entries and 15 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name VATB2_RAT
Isoform
PDB entities 2
Chains and sequence ranges Author chain D; PDBConstruct 1–511; UniProt 1–511 Author chain E; PDBConstruct 1–511; UniProt 1–511 Author chain F; PDBConstruct 1–511; UniProt 1–511

ATPase H+-transporting V1 subunit D

OrganismNot specified

UniProt Q6P503

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 17 PDB declaration: heptadecameric(17) Consistent with protein copy count Chain H; UniProt 1–247 Not recorded H(+)-transporting two-sector ATPase × 3 (D4A133) V-type proton ATPase subunit B, brain isoform × 3 (P62815) V-type proton ATPase subunit E 1 × 3 (Q6PCU2) V-type proton ATPase subunit F × 1 (P50408) V-type proton ATPase subunit G × 3 (Q8R2H0) SidK × 3 (Q5ZWW6) ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.4 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 3.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

19 other PDB entries and 19 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q6P503_RAT
Isoform
PDB entities 3
Chains and sequence ranges Author chain H; PDBConstruct 1–247; UniProt 1–247

V-type proton ATPase subunit E 1

OrganismNot specified

UniProt Q6PCU2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 17 PDB declaration: heptadecameric(17) Consistent with protein copy count Chain I; UniProt 1–226 Chain J; UniProt 1–226 Chain K; UniProt 1–226 Not recorded H(+)-transporting two-sector ATPase × 3 (D4A133) V-type proton ATPase subunit B, brain isoform × 3 (P62815) ATPase H+-transporting V1 subunit D × 1 (Q6P503) V-type proton ATPase subunit F × 1 (P50408) V-type proton ATPase subunit G × 3 (Q8R2H0) SidK × 3 (Q5ZWW6) ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.4 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 3.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

19 other PDB entries and 19 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name VATE1_RAT
Isoform
PDB entities 4
Chains and sequence ranges Author chain I; PDBConstruct 1–226; UniProt 1–226 Author chain J; PDBConstruct 1–226; UniProt 1–226 Author chain K; PDBConstruct 1–226; UniProt 1–226

V-type proton ATPase subunit F

OrganismNot specified

UniProt P50408

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 17 PDB declaration: heptadecameric(17) Consistent with protein copy count Chain L; UniProt 1–119 Not recorded H(+)-transporting two-sector ATPase × 3 (D4A133) V-type proton ATPase subunit B, brain isoform × 3 (P62815) ATPase H+-transporting V1 subunit D × 1 (Q6P503) V-type proton ATPase subunit E 1 × 3 (Q6PCU2) V-type proton ATPase subunit G × 3 (Q8R2H0) SidK × 3 (Q5ZWW6) ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.4 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 3.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

16 other PDB entries and 16 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name VATF_RAT
Isoform
PDB entities 5
Chains and sequence ranges Author chain L; PDBConstruct 1–119; UniProt 1–119

V-type proton ATPase subunit G

OrganismNot specified

UniProt Q8R2H0

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 17 PDB declaration: heptadecameric(17) Consistent with protein copy count Chain M; UniProt 1–118 Chain N; UniProt 1–118 Chain O; UniProt 1–118 Not recorded H(+)-transporting two-sector ATPase × 3 (D4A133) V-type proton ATPase subunit B, brain isoform × 3 (P62815) ATPase H+-transporting V1 subunit D × 1 (Q6P503) V-type proton ATPase subunit E 1 × 3 (Q6PCU2) V-type proton ATPase subunit F × 1 (P50408) SidK × 3 (Q5ZWW6) ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.4 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 3.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

13 other PDB entries and 13 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q8R2H0_RAT
Isoform
PDB entities 6
Chains and sequence ranges Author chain M; PDBConstruct 1–118; UniProt 1–118 Author chain N; PDBConstruct 1–118; UniProt 1–118 Author chain O; PDBConstruct 1–118; UniProt 1–118

SidK

Legionella pneumophila subsp. pneumophila str. Philadelphia 1

UniProt Q5ZWW6

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 17 PDB declaration: heptadecameric(17) Consistent with protein copy count Chain Q; UniProt 1–573 Chain R; UniProt 1–573 Chain S; UniProt 1–573 Not recorded H(+)-transporting two-sector ATPase × 3 (D4A133) V-type proton ATPase subunit B, brain isoform × 3 (P62815) ATPase H+-transporting V1 subunit D × 1 (Q6P503) V-type proton ATPase subunit E 1 × 3 (Q6PCU2) V-type proton ATPase subunit F × 1 (P50408) V-type proton ATPase subunit G × 3 (Q8R2H0) ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.4 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 3.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

22 other PDB entries and 22 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q5ZWW6_LEGPH
Isoform
PDB entities 7
Chains and sequence ranges Author chain Q; PDBConstruct 1–573; UniProt 1–573 Author chain R; PDBConstruct 1–573; UniProt 1–573 Author chain S; PDBConstruct 1–573; UniProt 1–573

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 9b8p

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 9b8p
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2. Structure Basics 2. Structure Basics

Entry ID entry_id9b8p
Deposition date deposition_date2024-03-31
Structure title titleSynaptic Vesicle V-ATPase with synaptophysin and SidK, State 3, V1
Keywords keywordsMmebrane, Synaptic, Complex, PROTON TRANSPORT; PROTON TRANSPORT
Experimental Method methodELECTRON MICROSCOPY

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier53.85
Radius of gyration Rg (electron density) rg_electron53.46
Forward intensity I(0) i04306510000.00
Molecular weight molecular_weight557480.0 kDa
Excluded volume excluded_volume700640 ų
Envelope volume envelope_volume953260 ų
Hydration-shell volume shell_volume139640 ų
Envelope diameter envelope_diameter179.5
Shell Rg shell_rg62.16
Envelope Rg envelope_rg53.62
Shape Rg shape_rg53.44
Total Rg total_rg53.72
Total atoms total_atoms39148
Residues n_residues4966
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 −1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax171.2
Rg (real space) rg_real53.63
Rg uncertainty (real space) rg_real_error1.12
I(0) (real space) i0_real4.3070e+09
I(0) uncertainty (real space) i0_real_error7.7590e+07
Rg (reciprocal space) rg_reciprocal54.02
I(0) (reciprocal space) i0_reciprocal4309000000.0000
Solution quality estimate total_estimate0.8735
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary64.9
Skewness Skewness skewness0.201
Kurtosis Kurtosis kurtosis-0.385
Angular range angular_range— – 0.1450 −1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha708400000.0000
Real-space data points n_real_points30
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.888; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.950; Smooth: 0.738

5. Crystallography and Experiment 5. Crystallography & Experiment

6. Entities and Polymers Entities & Polymers (8)

8. Citations (1)

9. Files and Curves (10)