9d2b

Symmetry-expanded reconstruction of augmin T-II bonsai on the microtubule

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Tubulin alpha-1B chain

OrganismNot specified

UniProt P81947

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 6	Tubulin beta-2B chain × 1 （Q6B856） HAUS augmin like complex subunit 6 L homeolog isoform X1 × 1 （A0A8J1MAE8） HAUS augmin like complex subunit 7 S homeolog × 1 （B1H1T5） HAUS augmin-like complex subunit 8 × 1 （Q0IHJ3） GUANOSINE-5'-TRIPHOSPHATE × 2 MAGNESIUM ION × 3 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	TBA1B_BOVIN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–451； UniProt 1–451 Author chain C； PDBConstruct 1–451； UniProt 1–451

Tubulin beta-2B chain

OrganismNot specified

UniProt Q6B856

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 6	Tubulin alpha-1B chain × 2 （P81947） HAUS augmin like complex subunit 6 L homeolog isoform X1 × 1 （A0A8J1MAE8） HAUS augmin like complex subunit 7 S homeolog × 1 （B1H1T5） HAUS augmin-like complex subunit 8 × 1 （Q0IHJ3） GUANOSINE-5'-TRIPHOSPHATE × 2 MAGNESIUM ION × 3 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	TBB2B_BOVIN
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–445； UniProt 1–445

HAUS augmin like complex subunit 6 L homeolog isoform X1

Xenopus laevis

UniProt A0A8J1MAE8

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 6	Tubulin alpha-1B chain × 2 （P81947） Tubulin beta-2B chain × 1 （Q6B856） HAUS augmin like complex subunit 7 S homeolog × 1 （B1H1T5） HAUS augmin-like complex subunit 8 × 1 （Q0IHJ3） GUANOSINE-5'-TRIPHOSPHATE × 2 MAGNESIUM ION × 3 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A8J1MAE8_XENLA
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain F； PDBConstruct 22–289； UniProt 1–247

HAUS augmin like complex subunit 7 S homeolog

Xenopus laevis

UniProt B1H1T5

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 6	Tubulin alpha-1B chain × 2 （P81947） Tubulin beta-2B chain × 1 （Q6B856） HAUS augmin like complex subunit 6 L homeolog isoform X1 × 1 （A0A8J1MAE8） HAUS augmin-like complex subunit 8 × 1 （Q0IHJ3） GUANOSINE-5'-TRIPHOSPHATE × 2 MAGNESIUM ION × 3 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	B1H1T5_XENLA
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain G； PDBConstruct 15–274； UniProt 2–261

HAUS augmin-like complex subunit 8

Xenopus laevis

UniProt Q0IHJ3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 6	Tubulin alpha-1B chain × 2 （P81947） Tubulin beta-2B chain × 1 （Q6B856） HAUS augmin like complex subunit 6 L homeolog isoform X1 × 1 （A0A8J1MAE8） HAUS augmin like complex subunit 7 S homeolog × 1 （B1H1T5） GUANOSINE-5'-TRIPHOSPHATE × 2 MAGNESIUM ION × 3 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	HAUS8_XENLA
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain H； PDBConstruct 4–260； UniProt 1–257

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	9d2b
Deposition date deposition_date	2024-08-08
Structure title title	Symmetry-expanded reconstruction of augmin T-II bonsai on the microtubule
Keywords keywords	Microtubule, augmin, CH, spindle, CELL CYCLE; CELL CYCLE
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

9d2b__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

9d2b__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

9d2b__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	41.00 Å
Rg (electron density)	41.01 Å
Total Rg	41.17 Å
Atom count	12067
Residues	1520
Excluded volume	213840 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`9d2b__assembly_1__model_1`	hexameric （6）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (8)

Entity 1 Entity 1 polymer

Description description	Tubulin alpha-1B chain
Molecular weight formula_weight	50204.4 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C,A
Atom count atom_count	12067

MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY

Entity 2 Entity 2 polymer

Description description	Tubulin beta-2B chain
Molecular weight formula_weight	49999.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATADEQGEFEEEEGEDEA

Entity 3 Entity 3 polymer

Description description	HAUS augmin like complex subunit 6 L homeolog isoform X1
Molecular weight formula_weight	33562.7 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

MGSSHHHHHHSGRENLYFQGSMQSGSRPHLAWQREHMWLALQGLGFESGAEAANAGKTLVHVTFGVNMFDKPNKDAFYVVFHFLFGKLDNVRCKEVFRYCWPPLDKKRDAEFRKACCEWLKKISDEVGAGFPQVVASIFLSPGGPKFVHLLYHFARYVMLQHIKRDADAGNVFISEALQSKIQDPQKALARNKLARQKYLKVLQKENLVIEEYQRKAQLLIKQIRDMRSEHVALQNQQKLAEKVDRKISDKDENIQKTRCMWNTIMQMLKEMEKEVDVVDAVVRGNIDQ

Source Source

Source organism organism	Xenopus laevis
Common name common_name	African clawed frog
NCBI taxonomy ID ncbi_taxonomy_id	8355
Host organism host_organism	Escherichia coli BL21

Entity 4 Entity 4 polymer

Description description	HAUS augmin like complex subunit 7 S homeolog
Molecular weight formula_weight	31848.1 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G

MADPWSHPQFEKGGTGGKELGAAVELYERLQMLSCPCLEGVYLTDPQSIYELLCTPSSHRLDILQWLCSRIYPPVQEQLSSLKESQTDTKVKEIAKLCFDLMLCHFDDLDLIRGHASPFKQISFIGQLLDVIQYPDTISSNVILESLSHSTEKNVVTCIRENEELLKELFSSPHFQATLSPECNPWPADFKPLLNAEESLQKRATQSSKGKDMSNSVEALLEISSSLKALKEECVDLCSSVTDGDKVIQSLRLALTDFHQLTIAFNQIYANEFQWSHPQFEK

Source Source

Source organism organism	Xenopus laevis
Common name common_name	African clawed frog
NCBI taxonomy ID ncbi_taxonomy_id	8355
Host organism host_organism	Escherichia coli BL21

Entity 5 Entity 5 polymer

Description description	HAUS augmin-like complex subunit 8
Molecular weight formula_weight	28742.9 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H

MRSMSEAGVAPIEDGSQNSSGGSSGDAALKKSKGGAKVVKSRYMQIGRSKVSKNSLANTTVCSGGKVPERGSGGTPTRRSLAPHKAKITAAVPLPALDGSIFTKEDLQSTLLDGHRIARPDLDLSVINDRTLQKITPRPVVTSEQKKPKRDTTPVNLVPEDMVEMIESQTLLLTYLTIKMQKNLFRLEEKAERNLLLVNDQKDQLQETIHMMKRDLTLLQREERLRDLIEKQDEVLTPVVTSKDPFKDNYTTFATALDST

Source Source

Source organism organism	Xenopus laevis
Common name common_name	African clawed frog
NCBI taxonomy ID ncbi_taxonomy_id	8355
Host organism host_organism	Escherichia coli BL21

Entity 6 Entity 6 non-polymer

Description description	GUANOSINE-5'-TRIPHOSPHATE
Molecular weight formula_weight	523.2 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 7 Entity 7 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	3

Entity 8 Entity 8 non-polymer

Description description	PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER
Molecular weight formula_weight	521.2 kDa
Source method src_method	syn
Copy count entity_count	1

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	triclinic

Resolution resolution	3.08 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	1772567

Symmetry-expanded reconstruction of augmin T-II bonsai on the microtubule

1. Protein Identity and Related Structures Protein Identity & Related Structures

Tubulin alpha-1B chain

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Tubulin beta-2B chain

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

HAUS augmin like complex subunit 6 L homeolog isoform X1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

HAUS augmin like complex subunit 7 S homeolog

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

HAUS augmin-like complex subunit 8

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

9d2b__assembly_1__model_1

9d2b__assembly_1__model_1 | I(q)

9d2b__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (8)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Source Source

Entity 4 Entity 4 polymer

Polymer Polymer

Source Source

Entity 5 Entity 5 polymer

Polymer Polymer

Source Source

Entity 6 Entity 6 non-polymer

Entity 7 Entity 7 non-polymer

Entity 8 Entity 8 non-polymer

7. Citations (1)