8fck

Structure of the vertebrate augmin complex

Method: ELECTRON MICROSCOPY D_max: 345.2 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

▼

1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8fck
沉积日期 deposition_date	2022-12-01
结构标题 title	Structure of the vertebrate augmin complex
关键词 keywords	microtubule, branching microtubule nucleation, spindle assembly, CELL CYCLE; CELL CYCLE
实验方法 method	ELECTRON MICROSCOPY

▼

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	— Å
回转半径 Rg (电子) rg_electron	132.10 Å
零角强度 I(0) i0	1750320000.00
分子量 molecular_weight	352520.0 kDa
排除体积 excluded_volume	440710 Å³
包络体积 envelope_volume	1190000 Å³
水化壳体积 shell_volume	85892 Å³
包络直径 envelope_diameter	473.2 Å
壳层 Rg shell_rg	79.54 Å
包络 Rg envelope_rg	131.20 Å
形状 Rg shape_rg	132.10 Å
总 Rg total_rg	131.40 Å
总原子数 total_atoms	24717
残基数 n_residues	3067
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

▼

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	345.2 Å
Rg (实空间) rg_real	117.70 Å
Rg 误差 (实空间) rg_real_error	2.63 Å
I(0) (实空间) i0_real	1.6710e+09
I(0) 误差 (实空间) i0_real_error	4.7510e+07
Rg (倒空间) rg_reciprocal	99.31 Å
I(0) (倒空间) i0_reciprocal	1598000000.0000
解质量估计 total_estimate	0.7818
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	3
主峰位置 r_peak_primary	115.1 Å
偏度 Skewness skewness	0.380
峰度 Kurtosis kurtosis	-0.906
角度范围 angular_range	— – 0.0600 Å⁻¹
当前正则化参数 α current_alpha	0.5023
最高正则化参数 α highest_alpha	76940000.0000
实空间数据点数 n_real_points	13
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.014; Oscil: 0.546; Stabil: 0.940; Sysdev: 1.000; Positv: 1.000; Valcen: 0.732; Smooth: 0.002

▶

4. 晶体学与实验 4. Crystallography & Experiment

▶

5. 实体与聚合体信息 (8)

Entity 1 polymer

描述 description	HAUS augmin-like complex subunit 1
分子量 formula_weight	32655.6 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A
原子数 atom_count	24717

MDEKSTKIIMWLKKMFGDKPLPPYEVNTRTMEILYQLAEWNEARDKDLSLVTEDLKLKSAEVKAEAKYLQDLLTEGLGPSYTNLSRMGNNYLNQIVDSCLALELKNSSLSSYIPAVNDLSSELVAIELNNQEMEAELTSLRKKLTEALVLEKSLEQDLKKAEEQCNFEKAKVEIRSQNMKKLKDKSEEYKYKIHAAKDQLSSAGMEEPLTHRSLVSLSETLTELKAQSMAAKEKLNSYLDLAPNPSLVKVKIEEAKRELKATEVELTTKVNMMEFVVPEPSKRRLK

来源 Source

来源生物 organism	Xenopus laevis
通用名 common_name	African clawed frog
NCBI 分类编号 ncbi_taxonomy_id	8355
宿主生物 host_organism	Spodoptera frugiperda

Entity 2 polymer

描述 description	HAUS augmin-like complex subunit 3
分子量 formula_weight	68112.2 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	B

MSGGDRFVQTLQKLNYPKGAQLDGEDFDWLFEAVDLKPFLDWFCSAASEQNVVPDEKLQAFNTLKESGKPVLDEKALDEVLKTFSISKVPAIEEVAIEKLEEEVKALQKQKNLHIRRRNKLQMVESGNRQMCLKSKDKEEETGRAFQEVLHLLRVTNKKLNHELQSIVNGVQTLMSFFSTPETACELSSQPIFLSQLLLDKYLSLEEQSTAALTSFTKEHFFEGMSKFVEGSDENFQLVQLNVNSFGEDGTTEDKCKEMMRLQLAYICAKHKLIQMKAKSASLKVGLQWAENNASVVQDKASQKEENLKVRITSLKNETLQIENHTNSISNEKLPGLVRDNAQLLNMPIVKGDYDLQMAHQTSCSSRQDLVCDHLMKQKASFELLQLGYELELRKHRDVYRELGSIVQELKESGDKLEERLTMLSDVNLLSASKPRSNIDSKDLTSHRLYQLLDGDNTQKLFRTYDGLESVAQKLSQDIASMRDQLEVSEQEHSLLLSKLDSHLKELRDFMYPEGNTLMLTTPELSGEFHQLGSQLEKLNHITVEILGDLQLKRKMLESNKLQQIEKQLYVYFFQNEEQLKSIVGKLEAQTGGGSSA

来源 Source

来源生物 organism	Xenopus laevis
通用名 common_name	African clawed frog
NCBI 分类编号 ncbi_taxonomy_id	8355
宿主生物 host_organism	Spodoptera frugiperda

Entity 3 polymer

描述 description	HAUS augmin like complex subunit 4 L homeolog
分子量 formula_weight	41256.4 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C

MAQTLQYVSSRLSMLQIDEEDLERNAQFGKVLIELCPLLGPNGGSANLNRELEETRRELLLQRKMWMRSEVIYQLVQEMLLDLQVRKLEGSLTEEERKFQDGLQQCMLVSECSRLLTADSVPPSDSTSILGLDKQDLLDLLPPNMLVLWVRDRLQKQLEEALKKKCFTFLSFHQPETDEEGDVLRAAKVLRLASTLEDEKRRLQNEQEKHQEMRALLEKQQEIYPHVLLRCLSLLRQAASELRLRAQSDIDRINAEYLEAKSNALFLKLRMEELQVLTDCYTPEKVLVHRQIRDTLEAGVKKEKQELSTSRQILSSYEFLGPEFEGLVQEYTRLKDKIKDNRWMLQELSKSLP

来源 Source

来源生物 organism	Xenopus laevis
通用名 common_name	African clawed frog
NCBI 分类编号 ncbi_taxonomy_id	8355
宿主生物 host_organism	Spodoptera frugiperda

Entity 4 polymer

描述 description	HAUS augmin-like complex subunit 5
分子量 formula_weight	77357.3 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	D

MERRSLAQELKKWAVEEMGLPAQKAPSEEMLQRLFIGQCGDIWKFIIRHIHSHRTVRKIEGNLLWYQQLQHTEAQRTAEEEQQQRRKQLCKEILELRAELHHLQEQIQTAEREIVGQDLNCERAQDLCRRSLLLRAFNKKREEECEALCQSNKKIQYRCEQLQEIRRASQREVMFSAVDPDLSSSTFLEPEVLRDVREVCKLRFKFLRSLHDDSISSSVHPGKEDLRSLSHQQWMSMAEKVWNTHTPNHILAALERLTLNSTQELKKLQFSQAADLSKGPSCQLKEFSEPITQSRSCNESTHLDPQETLPSFHSLIQEGWANSVKVSSELRRVQSQAQALSEHLAERIQEIHKKLSDGSEVSVLTRAAFDAELRCVILRGCRDALMQECRMLQEEAAGKKQEMKLLQQQQQNIQEACLLLDKKQKHIQILIKGNSSSKSQIRRSSVEAQKYVQDKLLPWPQEIIQESQRLQDSIQKEVKHFSAICLPALLKVSTDGFNLLPSRELSINRMSNTHAPYYGIFKGIYESVRLPLYKAPESVLSHVADMKKQLFFLRSQLSSRSEAISKTQRALQKNTNPDTDALLKSLSDHYSLELDEMVPKMQRLIQQCEKHQEYGKEVQATVMDWWEQPVQLCLPSEERGGLTLRQWRERWTVAVTALQRATGSRS

来源 Source

来源生物 organism	Xenopus laevis
通用名 common_name	African clawed frog
NCBI 分类编号 ncbi_taxonomy_id	8355
宿主生物 host_organism	Spodoptera frugiperda

Entity 5 polymer

描述 description	HAUS augmin like complex subunit 2 L homeolog, Green fluorescent protein chimera
分子量 formula_weight	53505.5 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E

MMAANPWSPVQPSAASLLLEKCLAAGVLSQNALDQAQLEAPCFSHAEELQRITEIKAEINQKSLELELLRLEKETADITHPLYLGQKHQALQAMNSHLEAVLREKRSLRQRLAQPVCQENLPIEASYHRFTAELLPLAVNFIEKLEIYIKTIQTIPKIPDCASNMDNALMRMESLEAEMEEVTEQILTWREQQKTAFQMNSDANSSCITAQTSYLSIENLHPVDMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKEFVTAAGITLGMDELYKVDHHHHHHH

来源 Source

来源生物 organism	Xenopus laevis
通用名 common_name	African clawed frog
NCBI 分类编号 ncbi_taxonomy_id	8355
宿主生物 host_organism	Spodoptera frugiperda

Entity 6 polymer

描述 description	HAUS augmin like complex subunit 6 L homeolog
分子量 formula_weight	50928.0 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	F

GPASGSTRGAEFMQSGSRPHLAWQREHMWLALQGLGFESGAEAANAGKTLVHVTFGVNMFDKPNKDAFYVVFHFLFGKLDNVRCKEVFRYCWPPLDKKRDAEFRKACCEWLKKISDEVGAGFPQVVASIFLSPGGPKFVHLLYHFARYVMLQHIKRDADAGNVFISEALQSKIQDPQKALARNKLARQKYLKVLQKENLVIEEYQRKAQLLIKQIRDMRSEHVALQNQQKLAEKVDRKISDKDENIQKTRCMWNTIMQMLKEMEKEVDVVDAVVRGNIDQYCLDGTNATLNIPNLLISRIESEMHRLQMDNVYEAGKVNLITVVQLLNEALKLVSGERSLYDCKGVRLDLQYLHGKAKFESEVLTRLRNMRHKIKREDLVSIEKIIADREREWERKWEKILGKCPFSLLKGLNPALELNPPMAPFSFDPASEEVLKSSVFCH

来源 Source

来源生物 organism	Xenopus laevis
通用名 common_name	African clawed frog
NCBI 分类编号 ncbi_taxonomy_id	8355
宿主生物 host_organism	Spodoptera frugiperda

Entity 7 polymer

描述 description	HAUS augmin like complex subunit 7 S homeolog
分子量 formula_weight	39379.6 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	G

MTGGKELGAAVELYERLQMLSCPCLEGVYLTDPQSIYELLCTPSSHRLDILQWLCSRIYPPVQEQLSSLKESQTDTKVKEIAKLCFDLMLCHFDDLDLIRGHASPFKQISFIGQLLDVIQYPDTISSNVILESLSHSTEKNVVTCIRENEELLKELFSSPHFQATLSPECNPWPADFKPLLNAEESLQKRATQSSKGKDMSNSVEALLEISSSLKALKEECVDLCSSVTDGDKVIQSLRLALTDFHQLTIAFNQIYANEFQEHCGHPAPHMSPMGPFFQFVHQSLSTCFKELESIAQFTETSENIVDVVRERHQSKEKWAGSTISTLCEKMKELRQSYEAFQQSSLQD

来源 Source

来源生物 organism	Xenopus laevis
通用名 common_name	African clawed frog
NCBI 分类编号 ncbi_taxonomy_id	8355
宿主生物 host_organism	Spodoptera frugiperda

Entity 8 polymer

描述 description	HAUS augmin-like complex subunit 8
分子量 formula_weight	41144.9 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	H

MSEAGVAPIEDGSQNSSGGSSGDAALKKSKGGAKVVKSRYMQIGRSKVSKNSLANTTVCSGGKVPERGSGGTPTRRSLAPHKAKITAAVPLPALDGSIFTKEDLQSTLLDGHRIARPDLDLSVINDRTLQKITPRPVVTSEQKKPKRDTTPVNLVPEDMVEMIESQTLLLTYLTIKMQKNLFRLEEKAERNLLLVNDQKDQLQETIHMMKRDLTLLQREERLRDLIEKQDEVLTPVVTSKDPFKDNYTTFATALDSTRHQLAIKNIHITGNRHRYLEELQKHLAITKSLLEEIMPSHASENAESFDTIKDLENIVLKTDEELARSFRQILDLSFKVNKEISLQSQKAVEETCESALVRQWYFDGSLP

来源 Source

来源生物 organism	Xenopus laevis
通用名 common_name	African clawed frog
NCBI 分类编号 ncbi_taxonomy_id	8355
宿主生物 host_organism	Spodoptera frugiperda

▶

7. 引用文献 (1)

▶

晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	6.88 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	114000

fit	/app/data/saxs_computation/fc/8fck/8fck.fit
pddf	/app/data/pr_computation/fc/8fck/8fck.pr.out
profile	/app/data/saxs_computation/fc/8fck/8fck.dat

Structure of the vertebrate augmin complex

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (8)

Entity 1 polymer

聚合体 Polymer

来源 Source

Entity 2 polymer

聚合体 Polymer

来源 Source

Entity 3 polymer

聚合体 Polymer

来源 Source

Entity 4 polymer

聚合体 Polymer

来源 Source

Entity 5 polymer

聚合体 Polymer

来源 Source

Entity 6 polymer

聚合体 Polymer

来源 Source

Entity 7 polymer

聚合体 Polymer

来源 Source

Entity 8 polymer

聚合体 Polymer

来源 Source

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)