Cryo-EM structure of PfHT1 bound to 2,5-anhydro-D-mannitol

Method: ELECTRON MICROSCOPY D_max: 104.5 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9sdl
deposition_date	2025-08-14
title	Cryo-EM structure of PfHT1 bound to 2,5-anhydro-D-mannitol
keywords	Sugar transporter, Plasmodium falciparum hexose transporter 1, TRANSPORT PROTEIN; TRANSPORT PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	32.87 Å
rg_electron	31.98 Å
i0	142965000.00
molecular_weight	107680.0 kDa
excluded_volume	139860 Å³
envelope_volume	169360 Å³
shell_volume	43230 Å³
envelope_diameter	105.6 Å
shell_rg	39.68 Å
envelope_rg	31.90 Å
shape_rg	31.99 Å
total_rg	32.66 Å
total_atoms	15452
n_residues	956
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	104.5 Å
rg_real	32.76 Å
rg_real_error	0.90 Å
i0_real	1.4300e+08
i0_real_error	2.2520e+06
rg_reciprocal	32.81 Å
i0_reciprocal	143000000.0000
total_estimate	0.9076
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	1
r_peak_primary	39.2 Å
skewness	0.189
kurtosis	-0.597
angular_range	— – 0.2400 Å⁻¹
current_alpha	0.0000
highest_alpha	30440000.0000
n_real_points	49
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.945; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.963

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	Hexose transporter 1,Green fluorescent protein
formula_weight	84639.9 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	15452

MEKEDSGFFSTSFKYVLSACIASFIFGYQVSVLNTIKNFIVVEFEWCKGEKDRLNCSNNTIQSSFLLASVFIGAVLGCGFSGYLVQFGRRLSLLIIYNFFFLVSILTSITHHFHTILFARLLSGFGIGLVTVSVPMYISEMTHKDKKGAYGVMHQLFITFGIFVAVMLGLAMGEGPKADSTEPLTSFAKLWWRLMFLFPSVISLIGILALVVFFKEETPYFLFEKGRIEESKNILKKIYETDNVDEPLNAIKEAVEQNESAKKNSLSLLSALKIPSYRYVIILGCLLSGLQQFTGINVLVSNSNELYKEFLDSHLITILSVVMTAVNFLMTFPAIYIVEKLGRKTLLLWGCVGVLVAYLPTAIANEINRNSNFVKILSIVATFVMIISFAVSYGPVLWIYLHEMFPSEIKDSAASLASLVNWVCAIIVVFPSDIIIKKSPSILFIVFSVMSILTFFFIFFFIKETKGGEIGTSPYITMEERQKHMTKSVVENLYFQGQFSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKDELYKHHHHHHHH

Source

organism	Aequorea victoria
ncbi_taxonomy_id	6100
host_organism	Saccharomyces cerevisiae

Entity 2 non-polymer

description	2.5-anhydro-D-mannitol
formula_weight	164.2 kDa
src_method	syn
entity_count	2

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	2.42 Å
resolution_method	—
num_particles	300678

fit	/app/data/saxs_computation/sd/9sdl/9sdl.fit
pddf	/app/data/pr_computation/sd/9sdl/9sdl.pr.out
profile	/app/data/saxs_computation/sd/9sdl/9sdl.dat