8fef

Structure of Mce1 transporter from Mycobacterium smegmatis (Map0)

Method: ELECTRON MICROSCOPY

SAXS 散射曲线 SAXS Profile

暂无 SAXS 图

P(r) 距离分布 P(r) Distribution

暂无 P(r) 图

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8fef
沉积日期 deposition_date	2022-12-06
结构标题 title	Structure of Mce1 transporter from Mycobacterium smegmatis (Map0)
关键词 keywords	Membrane protein complex, ABC transporter, Virulence factor, Lipid transport, MEMBRANE PROTEIN; MEMBRANE PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

该条目暂无 SAXS 数据。

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

该条目暂无 P(r) 分析数据。

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (10)

Entity 1 polymer

描述 description	Virulence factor Mce family protein
分子量 formula_weight	43944.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A
原子数 atom_count	25532

MTEPPAPTAPLNKPKTPPYKLAGLILGLVGVLVLALTWMQFRGQFEDKVQLTVLSGRAGLSMDPGSKVTFNGVPIGRLASIDVVEVDDNPEARLTLDVDPKYLDLIPENANVELRATTVFGNKYISFLSPKNPSAERLSASTPIRAQGVTTEFNTLFETITAISEQVDPIKLNETLTAAAQALDGLGDKFGRSIVDGNAILADVNPRMPQIRRDITGLANLGEVYADASPDLFDGLDNAVTTARTLNEQRGNLDQALVAAVGFGNTGGDIFERGGPYLVRGAQDLLPTSALLDEYSPALFCTIRNYHDAAPKLAGALGGNGYSLLTNSLVVGVGNPYVYPDNLPRVNAKGGPEGRPGCWQPITRDLWPFPYLVMDTGASIAPYNHFELGQPMFAEYVWGRQVGENTINP

Entity 2 polymer

描述 description	Virulence factor Mce family protein
分子量 formula_weight	37467.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	B

MSIKGTLFKLGIFSLVLLTFTALIFVVFGQIRFNRTTEYSAIFKNVSGLRDGQFVRAAGVEVGKVKSVDLINGGEQAEVKFTVERSLPLFQETTAAIRYQDLIGNRYLELKRGDSDQILPPGSTIPVERTEPALDLDALVGGFRPLFRSLEPEKVNTIATSLITIFQGQGGTINDILDQTAQLTASLADRDQAIGEVIKNLNTVLDTTVRHQKQFDETLVNFETLITGLKNRADPIATSVADISDAAGSLADLLSDNRPLLKDTIGYLDVIQAPLVEQKQEVSDILVQMPQALKIIGRAGGIYGDFFNFYACDLTLKLNGLQPGGPVRTVRITTQPSGRCTPK

Entity 3 polymer

描述 description	MCE-family protein MCE1c
分子量 formula_weight	54737.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C

MRTLQGSDRFRKGLMGVIVVALIIGVGSTLTSVPMLFAVPTYYGQFADTGGLNIGDKVRIAGMDVGNVKSMEIDGDKVVIGYTLGGRTIGTESRAAIRTDTILGRKNIEIEPRGSETLKPRGVLPVGQTSAPYQIYDAFLDVTRNAAGWDTQAVRQSLNVLSETVDQTSPHLSAALDGVARFSETIGKRDEDVKKLLASANKVATVLGDRSTQVNQLLVNAQTLLAAVNERGRSVSLLLERVSSVSRQVEGFVDENPNLNHVLEQLRTVSDVLNERKQDLADILTVAGKFITSLAEALASGPYFKVMLVNLIPPTILQPFVDAAFKKRGIDPEEFWRNAGLPAFRFPDPNGERHENGAPPAAPTPLEGTPEHPGPAVPPGSPCSYTPPADGIPSPGNPLPCAHLSQGPYGPVPGGYPPPNVATSAPNPDGIAHSPGVPSAAIPGQMPPEQPGAPVEIAPGPPGARTVPVSPIPGAPDFTPGIAPPPPAITGPPPPPGPGPQLAPVGEAPLPGNPPFLPPGSQSR

Entity 4 polymer

描述 description	Virulence factor mce family protein
分子量 formula_weight	58054.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	D

MSTIFNIRNIQLPRLSRAAVIIGALVVAAALVAGYFGMNAYRKLTNTTVTAYFPEVLALYPGDKVLIMGVRVGSIDSIETAGDKMKVVFHFNNKYKVPENATASILNPSLVASRVIQLSPPYTGGPTLRDGAVLDVDRTQVPIEYDEVRNQVTRLLADLGPTPEQPKGPFGDIIESFADGFAGKGEQLNRTLRGLSDALTALNEGRGDFFAVVKSLALFVNALHRSDQQFVALNNDLAQFTNSFTNTDQELANALQDLNRVLKTTREFLDRNGGVLTHDIDNLEQVTTAILQPEPRDGLETGLHAYPNLAANVLNINSPNQGGIIGLPVLPGVTNFSNPLQFVCSSIQAGSRLGYQESAELCAQYLAPIMDAIKFNYLPFGMNLASTAMTLPKQIAYSEKRLQPPPGYKDTTVPGIWSRDTLFSHGNHEPGWIVAPGMQGVQVQPATANMLTPESLAELLGGPDIVPPPAPPAFGTTRGGNLPGPPNAFDENNPLPPPWYPQPGPPPAPAPGVIPGDPLSAVAPAAPAAPAAPAPAGPPLPAEAGAG

Entity 5 polymer

描述 description	Virulence factor Mce family protein
分子量 formula_weight	42566.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E

MRLLKGFPKMRNWTRVGRRTAVLAAVALVLTSCGQWRGIANVPLPGGPGTESGSMTLYVQMPETLALNANSRVRVRDVFVGRVRKIELINWVPTLTVDVEPGIKLPKNTLAKIGQTSLLGSQHVELNPPEDPSSELLRDGDTIPLAQSSAYPTIERTLAGISGILTGGGIPNIEVIQTEVFNILNGRADQIREFLNQLDTFTDELNQQREEITRAIDSTNRLLNIVSQRNDTLDRVLTEFPPLIQHFAETRDLFADAVTALGRLSAAADETLSGSNANLHTNLQNLQRPLKQLGRAAPYLVGALKLILTVPFNIDNIPKAIRGDYINVSLKLDLTLSSVDNAFLSGTGVSGMLRALEQAWGRDPATMIPDVRFTPNPHDAPGGPLVERGE

Entity 6 polymer

描述 description	Mce-family protein mce1f
分子量 formula_weight	54342.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	F

MLLTRFIKMQLVIFLTLTLVALVVLALFYLRLPTWAGLGMYKLNADLPNSGGLYATANVTYRGTTIGKVTSVEPSESGARVEMNIYDRYKIPADATANVHSVSAVGEQFIDLTSDSGGGAYFQPGDTITKATVPAEVGPALDAAEKGLAVLPKEKIGTLLDEAATAFGGLGPSLQRLVDSTQAIAGDFRANIDPVNDIIENSGPIIDSQVNSGDAIQRWAANLNTLAAQSAQNDEALRSGLQQAAPTADQLNAVFSDVRESLPQTLANLEIVIDMLKRYNKNVEQVLVALPQGAAVAQTGTIFAPEGLLHFGLGINAPPPCLTGFLPASQWRSPADTRTEPLPSGLYCKIPKDAPNAVRGARNYPCADVPGKRAATPRECRSDEPYQPLGTNPWYGDPDQIRNCPAPGARCDQPVDPGRVIPAPSINNGLNPLPASQLPPPEVSSGPSSDPLTAPRGGTVTCSGQQPNPCIYTPAAGATATYNPASGEVVGPGGVKYSVTNSNTPGDDGWKEMLAPAS

Entity 7 polymer

描述 description	ABC transporter, ATP-binding protein,Green fluorescent protein chimera
分子量 formula_weight	71624.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	G,H

MGVQIDVTGLSKSFGSSKIWEDVTMSIPAGEVSVLLGPSGTGKSVFLKSLIGLLRPERGSIVIDGTDILQCSAKELYEIRTLFGVLFQDGALFGSMNIYDNTAFPLREHTKKSESEIRKIVMEKLDLVGMPNDGHKFPGEISGGMRKRAGLARALVLDPEIILCDEPDSGLDPVRTAYLSQLLIDINAQIDATVLIVTHNINIARTVPDNMGMLFRKQLVMFGPREVLLTSEEPVVKQFLNGRRIGPIGMSEEKDEATAAAEQALVDAGQHDGGVEEIEGVPPQLQATPGMPERKAVARRKARVREILHTLPPAAQAAILEELDRDQPQLSAPTTQTAATAPVENYDDSPTGVIEVPKQAGLSGQPPRSPSSGSSSNSLEVLFQGPTAAAAVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGGGENLYFQDYKDDDDKHHHHHH

Entity 8 polymer

描述 description	Conserved hypothetical integral membrane protein Yrbe1a
分子量 formula_weight	27674.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	I

MTASTDGFVDYLRGQLEKPLATVGGFFKMSVMTGKALFTRPFQWKEFVLQSWFLIRVAFLPTLAVSIPLTVLIIFTLNILLAEFGAADVSGAGAALGAVTQLGPLVTVLVVAGAGSTAICADLGARTVREEIDALEVLGIDPIERLVVPRVVASTFVAFMLNGAVITIGLVGGFFFGVYIQNVSAGAYVSTLTLLTGFPEVLISVVKATLFGMIAGLVGCYRGLTVAGGSKGVGTAVNETLVLCVVALFAVNVVLTTIGVRFGTGR

Entity 9 polymer

描述 description	ABC-transporter integral membrane protein
分子量 formula_weight	30809.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	J

MSTVQVLRSRFPRAFSRSSEIAATPARFLDSMGHVAWFVVQAIVHVPHAFRHYRRESLRLVAEIGMGTGAMAVIGGTVAIIGFVTLSAGSLIAIQGFASLGNIGVEAFTGFFAALANIRVVAPVVTGQALAATVGAGATAELGAMRISEEVDALEVMGIKSISYLVSTRIMAGAIVIIPLYAMAILLSFMSAQLVTTIFYSQSVGTYEHYFHTFLRVDDVMWSFLEVIIMSVIVMLNHCYFGYFASGGAVGVGEAVGRSMRTSLIAIVLVVLLASLALYGTDPNFNLTV

Entity 10 non-polymer

描述 description	UNKNOWN LIGAND
来源方法 src_method	syn
拷贝数 entity_count	31

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6. 折叠分类 (SCOP + CATH) 2 domains

CATH v4.4 (2 domains)

结构域编号 domain_id	8fefG01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

结构域编号 domain_id	8fefH01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	2.71 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	305172

Structure of Mce1 transporter from Mycobacterium smegmatis (Map0)

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (10)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 non-polymer

6. 折叠分类 (SCOP + CATH) 2 domains

CATH v4.4 (2 domains)

7. 引用文献 (1)

8. 文件与曲线 (0)