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6wrf

ClpX-ClpP complex bound to GFP-ssrA, recognition complex

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

ATP-dependent Clp protease ATP-binding subunit ClpX

Escherichia coli (strain K12)

UniProt P0A6H1

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 14 ATP-dependent Clp protease proteolytic subunit × 7 （P0A6G7） Green fluorescent protein × 1 （P42212） PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 5 MAGNESIUM ION × 5 ADENOSINE-5'-DIPHOSPHATE × 1 water × 7 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer
1ovx 2ds5 2ds6 2ds7

4 assemblies

Homotetramer

None

Other homooligomers
3hte 3hws 3hws 3hws 4i34 4i4l 4i5o 4i63 4i81 4i9k

10 assemblies

Heteromeric or nucleic-acid complex
2ds8 6wr2 6wrf 6wsg 8e7v 8e8q 8v9r 9c87 9c88

9 assemblies

View Construct and Data Evidence
UniProt name CLPX_ECOLI
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 26–388； UniProt 62–424 Author chain B； PDBConstruct 26–388； UniProt 62–424 Author chain C； PDBConstruct 26–388； UniProt 62–424 Author chain D； PDBConstruct 26–388； UniProt 62–424 Author chain E； PDBConstruct 26–388； UniProt 62–424 Author chain F； PDBConstruct 26–388； UniProt 62–424

ATP-dependent Clp protease proteolytic subunit

Escherichia coli (strain K12)

UniProt P0A6G7

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 14 ATP-dependent Clp protease ATP-binding subunit ClpX × 6 （P0A6H1） Green fluorescent protein × 1 （P42212） PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 5 MAGNESIUM ION × 5 ADENOSINE-5'-DIPHOSPHATE × 1 water × 7 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers
1tyf 1yg6 2fzs 3hln 3hln 6nb1 7mk5 7mk5

8 assemblies

Heteromeric or nucleic-acid complex
3mt6 3mt6 6wr2 6wrf 6wsg 8e7v 8e8q 8e91 8et3 8v9r 9c87 9c88

14 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name CLPP_ECOLI
Isoform —
PDB entities 2
Chains and sequence ranges Author chain H； PDBConstruct 1–192； UniProt 16–207 Author chain I； PDBConstruct 1–192； UniProt 16–207 Author chain J； PDBConstruct 1–192； UniProt 16–207 Author chain K； PDBConstruct 1–192； UniProt 16–207 Author chain L； PDBConstruct 1–192； UniProt 16–207 Author chain M； PDBConstruct 1–192； UniProt 16–207 Author chain N； PDBConstruct 1–192； UniProt 16–207

Green fluorescent protein

Aequorea victoria

UniProt P42212

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 14 ATP-dependent Clp protease ATP-binding subunit ClpX × 6 （P0A6H1） ATP-dependent Clp protease proteolytic subunit × 7 （P0A6G7） PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 5 MAGNESIUM ION × 5 ADENOSINE-5'-DIPHOSPHATE × 1 water × 7 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
1bfp 1c4f 1cv7 1ema 1emb 1emg 1f09 1f0b 1gfl 1gfl 1h6r 1h6r

379 assemblies; showing the first 12

Homodimer
1eme 1emf 1emk 1eml 1emm 1kp5 1kp5 1kp5 1myw 2b3q 2b3q 2emd

31 assemblies; showing the first 12

Homotetramer
1b9c 1emc 1hcj 1hcj 1kp5 1kp5 1w7s 1w7t 2ah8 5mse

10 assemblies

Other homooligomers
1w7u 5hbd 5hge 5hw9 6as9 6mdr

6 assemblies

Heteromeric or nucleic-acid complex
2jad 3ek4 3ek7 3ek8 3ekh 3ekj 3evr 3evu 3evv 3g9a 3k1k 3k1k

319 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name GFP_AEQVI
Isoform —
PDB entities 3
Chains and sequence ranges Author chain S； PDBConstruct 27–253； UniProt 3–229

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id6wrf
Deposition date deposition_date2020-04-29
Structure title titleClpX-ClpP complex bound to GFP-ssrA, recognition complex
Keywords keywordsProtein degradation, AAA+ protease complex, CHAPERONE; CHAPERONE
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

6wrf__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

6wrf__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

6wrf__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）48.10 Å
Rg (electron density)47.10 Å
Total Rg47.32 Å
Atom count53916
Residues3450
Excluded volume479450 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 6wrf__assembly_1__model_1 tetradecameric （14） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (7)

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6. Fold Classification (SCOP + CATH) 14 domains

SCOP 2.08 (14 domains)

Domain ID domain_idd6wrfa_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
Superfamily Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
Family Family familyc.37.1.20 — Extended AAA-ATPase domain
Domain ID domain_idd6wrfb_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
Superfamily Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
Family Family familyc.37.1.20 — Extended AAA-ATPase domain
Domain ID domain_idd6wrfc_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
Superfamily Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
Family Family familyc.37.1.20 — Extended AAA-ATPase domain
Domain ID domain_idd6wrfd_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
Superfamily Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
Family Family familyc.37.1.20 — Extended AAA-ATPase domain
Domain ID domain_idd6wrfe_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
Superfamily Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
Family Family familyc.37.1.20 — Extended AAA-ATPase domain
Domain ID domain_idd6wrff_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
Superfamily Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
Family Family familyc.37.1.20 — Extended AAA-ATPase domain
Domain ID domain_idd6wrfh_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.14 — ClpP/crotonase
Superfamily Superfamily superfamilyc.14.1 — ClpP/crotonase
Family Family familyc.14.1.1 — Clp protease, ClpP subunit
Domain ID domain_idd6wrfi_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.14 — ClpP/crotonase
Superfamily Superfamily superfamilyc.14.1 — ClpP/crotonase
Family Family familyc.14.1.1 — Clp protease, ClpP subunit
Domain ID domain_idd6wrfj_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.14 — ClpP/crotonase
Superfamily Superfamily superfamilyc.14.1 — ClpP/crotonase
Family Family familyc.14.1.1 — Clp protease, ClpP subunit
Domain ID domain_idd6wrfk_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.14 — ClpP/crotonase
Superfamily Superfamily superfamilyc.14.1 — ClpP/crotonase
Family Family familyc.14.1.1 — Clp protease, ClpP subunit
Domain ID domain_idd6wrfl_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.14 — ClpP/crotonase
Superfamily Superfamily superfamilyc.14.1 — ClpP/crotonase
Family Family familyc.14.1.1 — Clp protease, ClpP subunit
Domain ID domain_idd6wrfm1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.14 — ClpP/crotonase
Superfamily Superfamily superfamilyc.14.1 — ClpP/crotonase
Family Family familyc.14.1.1 — Clp protease, ClpP subunit
Domain ID domain_idd6wrfm2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd6wrfn_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.14 — ClpP/crotonase
Superfamily Superfamily superfamilyc.14.1 — ClpP/crotonase
Family Family familyc.14.1.1 — Clp protease, ClpP subunit
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7. Citations (1)