Bipartite Genetically Encoded Biosensor sG-GECO1

entry_id	9u9d
deposition_date	2025-03-27
title	Bipartite Genetically Encoded Biosensor sG-GECO1
keywords	Fluorescent protein, GFP, bipartite scpFP, sG-GECO1; FLUORESCENT PROTEIN
method	X-RAY DIFFRACTION

rg_guinier	18.42 Å
rg_electron	17.16 Å
i0	12100200.00
molecular_weight	26058.0 kDa
excluded_volume	32671 ų
envelope_volume	36697 ų
shell_volume	17732 ų
envelope_diameter	60.7 Å
shell_rg	23.58 Å
envelope_rg	17.60 Å
shape_rg	17.14 Å
total_rg	18.24 Å
total_atoms	1839
n_residues	230
n_harmonics	20
q_range	— – 0.5000 Å−1
n_points	101
shell_type	directional
solvent_density	0.3340 e/ų
contrast_shell	0.0300 e/ų
crysol_version	4.1.3

dmax	59.3 Å
rg_real	18.33 Å
rg_real_error	0.22 Å
i0_real	1.2100e+07
i0_real_error	1.3740e+05
rg_reciprocal	18.34 Å
i0_reciprocal	12100000.0000
total_estimate	0.6579
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	23.6 Å
skewness	0.228
kurtosis	-0.345
angular_range	— – 0.4300 Å−1
current_alpha	0.0046
highest_alpha	4130000.0000
n_real_points	74
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.852; Stabil: 1.000; Sysdev: 0.000; Positv: 1.000; Valcen: 0.997; Smooth: 0.998

Unit Cell

length_a	51.104 Å
length_b	51.104 Å
length_c	201.306 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

Symmetry

space_group	P 41 21 2
int_tables_number	92
crystal_system	—

Refinement

resolution	1.800 Å
resolution_low	36.140 Å
r_factor	0.1868
r_work	0.1835
r_free	0.2272

Diffraction

temperature

100.0 K

Files (7)

• pr_distribution /app/data/pr_computation/u9/9u9d/pr_distribution.json
• pr_output /app/data/pr_computation/u9/9u9d/9u9d.pr.out
• pr_plot /app/data/visualization/pr_computation/u9/9u9d/9u9d_pr_distribution.png
• saxs_curve_dat /app/data/saxs_computation/u9/9u9d/9u9d.dat
• saxs_fit /app/data/saxs_computation/u9/9u9d/9u9d.fit
• saxs_log /app/data/saxs_computation/u9/9u9d/9u9d.log
• saxs_plot /app/data/visualization/saxs_computation/u9/9u9d/9u9d_saxs_profile.png

Curves (3)

fit	/app/data/saxs_computation/u9/9u9d/9u9d.fit
pddf	/app/data/pr_computation/u9/9u9d/9u9d.pr.out
profile	/app/data/saxs_computation/u9/9u9d/9u9d.dat