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4eul

Crystal structure of enhanced Green Fluorescent Protein to 1.35A resolution reveals alternative conformations for Glu222

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Green fluorescent protein

Aequorea victoria

UniProt P42212

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein monomer Monomer Protein 1 CALCIUM ION × 2 DI(HYDROXYETHYL)ETHER × 4 SULFATE ION × 1 water × 1 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
1bfp 1c4f 1cv7 1ema 1emb 1emg 1f09 1f0b 1gfl 1gfl 1h6r 1h6r

379 assemblies; showing the first 12

Homodimer
1eme 1emf 1emk 1eml 1emm 1kp5 1kp5 1kp5 1myw 2b3q 2b3q 2emd

31 assemblies; showing the first 12

Homotetramer
1b9c 1emc 1hcj 1hcj 1kp5 1kp5 1w7s 1w7t 2ah8 5mse

10 assemblies

Other homooligomers
1w7u 5hbd 5hge 5hw9 6as9 6mdr

6 assemblies

Heteromeric or nucleic-acid complex
2jad 3ek4 3ek7 3ek8 3ekh 3ekj 3evr 3evu 3evv 3g9a 3k1k 3k1k

319 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name GFP_AEQVI
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 3–237； UniProt 2–238

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id4eul
Deposition date deposition_date2012-04-25
Structure title titleCrystal structure of enhanced Green Fluorescent Protein to 1.35A resolution reveals alternative conformations for Glu222
Keywords keywordsbeta barrel, cyclization of T65, Y66, G67 backbone atoms, generating chromophore, FLUORESCENT PROTEIN; FLUORESCENT PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

4eul__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

4eul__assembly_1__model_1 | I(q)

10-2 10-1 105 106 107 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

4eul__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）18.29 Å
Rg (electron density)17.08 Å
Total Rg18.23 Å
Atom count1853
Residues226
Excluded volume32995 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 4eul__assembly_1__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (5)

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6. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

Domain ID domain_idd4eula_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.22 — GFP-like
Superfamily Superfamily superfamilyd.22.1 — GFP-like
Family Family familyd.22.1.1 — Fluorescent proteins

CATH v4.4 (1 domains)

Domain ID domain_id4eulA00
Class class2 — Mainly Beta
Architecture architecture40 — Beta Barrel
Topology topology155 — Green Fluorescent Protein
Homologous superfamily homologous superfamily10 — Green fluorescent protein
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7. Citations (1)