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9dhz

Cryo-EM structure of HURP bound to a microtubule

Method: ELECTRON MICROSCOPY Dmax: 128.5 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Disks large-associated protein 5

Homo sapiens

UniProt Q15398

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 5 PDB declaration: pentameric（5） Consistent with protein copy count Chain C； UniProt 1–285 Not recorded Tubulin beta chain × 2 （P02554） Tubulin alpha-1B chain × 2 （Q2XVP4） TA1 TAXOL × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 6.9 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.10 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9DHZ Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7ZX4 Clathrin N-terminal domain in complex with a HURP phospho-peptide Deposited 2022-05-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 826–846（21 aa）
Chain E 826–846（21 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) GOL GLYCEROL × 2 CL CHLORIDE ION × 1 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.5；293 K；50 mM Tris pH 7.5, 30 % PEG 6000
Resolution 2.08 Å R-free 0.249
7ZX4 Clathrin N-terminal domain in complex with a HURP phospho-peptide Deposited 2022-05-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 826–846（21 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.5；293 K；50 mM Tris pH 7.5, 30 % PEG 6000
Resolution 2.08 Å R-free 0.249
8X9P HURP (428-534)-alpha-tubulin-beta-tubulin complex Deposited 2023-11-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 199–619（421 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
9DUQ HURP(65-174) bound to GMPCPP-stabilized microtubule Deposited 2024-10-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric
Chain r 87–132（46 aa） Fragment：UNP residues 87-132
Chain s 87–132（46 aa） Fragment：UNP residues 87-132
Chain t 87–132（46 aa） Fragment：UNP residues 87-132
Chain u 87–132（46 aa） Fragment：UNP residues 87-132
Chain v 87–132（46 aa） Fragment：UNP residues 87-132
Chain w 87–132（46 aa） Fragment：UNP residues 87-132
Chain x 87–132（46 aa） Fragment：UNP residues 87-132
Chain y 87–132（46 aa） Fragment：UNP residues 87-132
Chain z 87–132（46 aa） Fragment：UNP residues 87-132
Not recorded G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 9 MG MAGNESIUM ION × 18 GTP GUANOSINE-5'-TRIPHOSPHATE × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 (1X): 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å

3 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name DLGP5_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain C； PDBConstruct 7–291； UniProt 1–285

Tubulin beta chain

OrganismNot specified

UniProt P02554

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 5 PDB declaration: pentameric（5） Consistent with protein copy count Chain A； UniProt 1–445 Chain K； UniProt 1–445 Not recorded Disks large-associated protein 5 × 1 （Q15398） Tubulin alpha-1B chain × 2 （Q2XVP4） TA1 TAXOL × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 6.9 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.10 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9DHZ Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1FFX TUBULIN:STATHMIN-LIKE DOMAIN COMPLEX Deposited 2000-07-26 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 293.K
Resolution 3.95 Å R-free 0.367
1IA0 KIF1A HEAD-MICROTUBULE COMPLEX STRUCTURE IN ATP-FORM Deposited 2001-03-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TXL TAXOTERE × 1 MG MAGNESIUM ION × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer buf1；pH 7.4；IMIDAZOLE 50 MM, MG-ACETATE 5 MM, EGTA 1 MM, K-ACETATE 50 MM, DTT 10 MM, PACLITAXEL 10 MICRO M
cryo-EM vitrification conditions PLUNGED INTO ETHANE
X-ray crystallization conditions polymerization of the microtubule；pH 6.8；296 K；pH 6.8, polymerization of the microtubule, temperature 296K
Resolution 15.00 Å
1SA0 TUBULIN-COLCHICINE: STATHMIN-LIKE DOMAIN COMPLEX Deposited 2004-02-06 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；PEG, PIPES BUFFER, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 3.58 Å R-free 0.249
1SA1 Tubulin-podophyllotoxin: stathmin-like domain complex Deposited 2004-02-06 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain E 49–189（141 aa）
Not recorded MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 POD 9-HYDROXY-5-(3,4,5-TRIMETHOXYPHENYL)-5,8,8A,9-TETRAHYDROFURO[3',4':6,7]NAPHTHO[2,3-D][1,3]DIOXOL-6(5AH)-ONE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；PEG, PIPES BUFFER, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 4.20 Å R-free 0.259
1TUB TUBULIN ALPHA-BETA DIMER, ELECTRON DIFFRACTION Deposited 1997-09-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–427（427 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TXL TAXOTERE × 1 ELECTRON CRYSTALLOGRAPHY
X-ray crystallization conditions TWO-DIMENSIONAL SHEETS ARE FORMED UNDER NORMAL POLYMERIZING CONDITIONS WITH THE ADDITION OF 1 MM ZNCL2
Resolution 3.70 Å
1TVK The binding mode of epothilone A on a,b-tubulin by electron crystallography Deposited 2004-06-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–427（427 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 EP EPOTHILONE A × 1 ELECTRON CRYSTALLOGRAPHY mmCIF provides none of the parsed conditions Resolution 2.89 Å R-free 0.321
2HXF KIF1A head-microtubule complex structure in amppnp-form Deposited 2006-08-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer IMIDAZOLE；pH 7.4；IMIDAZOLE
cryo-EM vitrification conditions Ethan slash
Resolution 10.00 Å
2HXH KIF1A head-microtubule complex structure in adp-form Deposited 2006-08-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer imidazole；pH 7.4；imidazole
cryo-EM vitrification conditions Ethan slash
Resolution 11.00 Å
2WBE Kinesin-5-Tubulin Complex with AMPPNP Deposited 2009-02-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer 80MM PIPES, 150MM NACL, 7MM MGCL2, 1MM EGTA, 1MM BETA-MERCAPTOETHANOL；pH 6.8；80MM PIPES, 150MM NACL, 7MM MGCL2, 1MM EGTA, 1MM BETA-MERCAPTOETHANOL
cryo-EM vitrification conditions ETHANE
Resolution 9.40 Å
3EDL Kinesin13-Microtubule Ring complex Deposited 2008-09-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 165 PDB declaration: 165-meric
Chain B 1–445（445 aa）
Chain G 1–445（445 aa）
Not recorded ZN ZINC ION × 33 MG MAGNESIUM ION × 99 GTP GUANOSINE-5'-TRIPHOSPHATE × 66 GDP GUANOSINE-5'-DIPHOSPHATE × 66 TA1 TAXOL × 33 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 33 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 33 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；80 mM Pipes, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 2 mM AMP-PNP, 0.02 mM taxol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 28.00 Å
3EDL Kinesin13-Microtubule Ring complex Deposited 2008-09-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain G 1–445（445 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；80 mM Pipes, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 2 mM AMP-PNP, 0.02 mM taxol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 28.00 Å
3EDL Kinesin13-Microtubule Ring complex Deposited 2008-09-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain G 1–445（445 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；80 mM Pipes, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 2 mM AMP-PNP, 0.02 mM taxol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 28.00 Å
3J6E Energy minimized average structure of Microtubules stabilized by GmpCpp Deposited 2014-02-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain B 1–427（427 aa）
Chain D 1–427（427 aa）
Chain F 1–427（427 aa）
Chain H 1–427（427 aa）
Chain J 1–427（427 aa）
Chain L 1–427（427 aa）
Chain N 1–427（427 aa）
Chain P 1–427（427 aa）
Chain R 1–427（427 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 18 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 9 ELECTRON MICROSCOPY
cryo-EM buffer 80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions The grid was blotted for 2 seconds before plunging.；90.4 K；Cryogen ETHANE；The grid was blotted for 2 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 4.70 Å
3J6F Minimized average structure of GDP-bound dynamic microtubules Deposited 2014-02-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain B 1–427（427 aa）
Chain D 1–427（427 aa）
Chain F 1–427（427 aa）
Chain H 1–427（427 aa）
Chain J 1–427（427 aa）
Chain L 1–427（427 aa）
Chain N 1–427（427 aa）
Chain P 1–427（427 aa）
Chain R 1–427（427 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 ELECTRON MICROSCOPY
cryo-EM buffer 80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions The grid was blotted for 2 seconds before plunging.；90.4 K；Cryogen ETHANE；The grid was blotted for 2 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 4.90 Å
3J6G Minimized average structure of microtubules stabilized by taxol Deposited 2014-02-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain B 1–427（427 aa）
Chain D 1–427（427 aa）
Chain F 1–427（427 aa）
Chain H 1–427（427 aa）
Chain J 1–427（427 aa）
Chain L 1–427（427 aa）
Chain N 1–427（427 aa）
Chain P 1–427（427 aa）
Chain R 1–427（427 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 TA1 TAXOL × 9 ELECTRON MICROSCOPY
cryo-EM buffer 80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions The grid was blotted for 2 seconds before plunging.；90.4 K；Cryogen ETHANE；The grid was blotted for 2 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 5.50 Å
3J6H Nucleotide-free Kinesin motor domain complexed with GMPCPP-microtubule Deposited 2014-02-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 2–427（426 aa） Fragment：UNP residues 2-427
Not recorded MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 SO4 SULFATE ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.10 Å
3J6P Pseudo-atomic model of dynein microtubule binding domain-tubulin complex based on a cryoEM map Deposited 2014-03-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Blot for 3.5 seconds before plunging；Cryogen ETHANE
Resolution 8.20 Å
3J7I Structure of alpha- and beta- tubulin in GMPCPP-microtubules Deposited 2014-07-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.90 Å
3JAK Cryo-EM structure of GTPgammaS-microtubule co-polymerized with EB3 (merged dataset with and without kinesin bound) Deposited 2015-06-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions Blot once for 4 seconds before plunging.；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 3.30 Å
3JAL Cryo-EM structure of GMPCPP-microtubule co-polymerized with EB3 Deposited 2015-06-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 ELECTRON MICROSCOPY
cryo-EM buffer BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions Blot once for 4 seconds before plunging；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 3.50 Å
3JAR Cryo-EM structure of GDP-microtubule co-polymerized with EB3 Deposited 2015-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions Blot once for 4 seconds before plunging/；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 3.40 Å
3JAS Cryo-EM structure of dynamic GDP-microtubule (14 protofilaments) decorated with kinesin Deposited 2015-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions Blot once for 4 seconds before plunging.；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 3.50 Å
3JAT Cryo-EM structure of GMPCPP-microtubule (14 protofilaments) decorated with kinesin Deposited 2015-06-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 12 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 ELECTRON MICROSCOPY
cryo-EM buffer BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions Blot once for 4 seconds before plunging.；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 3.50 Å
3JAW Atomic model of a microtubule seam based on a cryo-EM reconstruction of the EB3-bound microtubule (merged dataset containing tubulin bound to GTPgammaS, GMPCPP, and GDP) Deposited 2015-06-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions Blot once for 4 seconds before plunging.；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 3.90 Å
4ZHQ Crystal structure of Tubulin-Stathmin-TTL-MMAE Complex Deposited 2015-04-26 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 GOL GLYCEROL × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 4Q5 N-methyl-L-valyl-N-[(3R,4S,5S)-1-{(2S)-2-[(1R,2R)-3-{[(1S,2R)-1-hydroxy-1-phenylpropan-2-yl]amino}-1-methoxy-2-methyl-3-oxopropyl]pyrrolidin-1-yl}-3-methoxy-5-methyl-1-oxoheptan-4-yl]-N-methyl-L-valinamide × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.7；293 K；6% PEG, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 2.55 Å R-free 0.245
4ZI7 CRYSTAL STRUCTURE OF TUBULIN-STATHMIN-TTL-HTI286 COMPLEX Deposited 2015-04-27 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 4 GOL GLYCEROL × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 4SL N,beta,beta-trimethyl-L-phenylalanyl-N-[(3S,4Z)-5-carboxy-2-methylhex-4-en-3-yl]-N,3-dimethyl-L-valinamide × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；293 K；PEG, glycerol, MES, CaCl2, MgCl2
Resolution 2.51 Å R-free 0.239
4ZOL Crystal Structure of Tubulin-Stathmin-TTL-Tubulysin M Complex Deposited 2015-05-06 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 55Q (2R,4R)-4-{[(2-{(1R,3R)-1-(acetyloxy)-4-methyl-3-[methyl(N-{[(2S)-1-methylpiperidin-2-yl]carbonyl}-D-isoleucyl)amino]pentyl}-1,3-thiazol-4-yl)carbonyl]amino}-2-methyl-5-phenylpentanoic acid × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.7；293 K；6% PEG, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 2.50 Å R-free 0.232
5BMV CRYSTAL STRUCTURE OF TUBULIN-STATHMIN-TTL-Vinblastine COMPLEX Deposited 2015-05-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 GOL GLYCEROL × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 VLB (2ALPHA,2'BETA,3BETA,4ALPHA,5BETA)-VINCALEUKOBLASTINE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；293 K；6% PEG, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 2.50 Å R-free 0.235
5FNV a new complex structure of tubulin with an alpha-beta unsaturated lactone Deposited 2015-11-16 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X3H PIRONETIN × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.7；0.1M MES PH6.7;6% PEG4000 ;5% GLYCEROL;30MM CACL2;30MM MGCL2
Resolution 2.61 Å R-free 0.226
5JQG An apo tubulin-RB-TTL complex structure used for side-by-side comparison Deposited 2016-05-04 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.7；293 K；0.1M MES, 6% PEG4000, 5% Glycerol, 30mM CaCl2, 30mM MgCl2
Resolution 2.24 Å R-free 0.212
5KMG Near-atomic cryo-EM structure of PRC1 bound to the microtubule Deposited 2016-06-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain B 1–431（431 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 14 MG MAGNESIUM ION × 14 GDP GUANOSINE-5'-DIPHOSPHATE × 14 POU Peloruside A × 14 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot for 4 seconds, blot force 10
Resolution 3.50 Å
5KMG Near-atomic cryo-EM structure of PRC1 bound to the microtubule Deposited 2016-06-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–431（431 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 POU Peloruside A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot for 4 seconds, blot force 10
Resolution 3.50 Å
5KMG Near-atomic cryo-EM structure of PRC1 bound to the microtubule Deposited 2016-06-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–431（431 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 POU Peloruside A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot for 4 seconds, blot force 10
Resolution 3.50 Å
5MM4 Ustilago maydis kinesin-5 motor domain in the AMPPNP state bound to microtubules Deposited 2016-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–427（427 aa）
Not recorded MG MAGNESIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 TA1 TAXOL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
5MM7 Ustilago maydis kinesin-5 motor domain with N-terminal extension in the AMPPNP state bound to microtubules Deposited 2016-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–427（427 aa）
Not recorded MG MAGNESIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 TA1 TAXOL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.10 Å
5OAM Molecular basis of human kinesin-8 function and inhibition Deposited 2017-06-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.50 Å
5OCU Molecular basis of human kinesin-8 function and inhibition Deposited 2017-07-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.20 Å
5OGC Molecular basis of human kinesin-8 function and inhibition Deposited 2017-07-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded 9V5 4-chloranyl-2-nitro-1-(phenylsulfonyl)benzene × 1 ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.80 Å
5SYC Near-atomic resolution cryo-EM reconstruction of peloruside-stabilized microtubule Deposited 2016-08-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 52 PDB declaration: 52-meric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 26 MG MAGNESIUM ION × 26 GDP GUANOSINE-5'-DIPHOSPHATE × 26 POU Peloruside A × 26 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYC Near-atomic resolution cryo-EM reconstruction of peloruside-stabilized microtubule Deposited 2016-08-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 POU Peloruside A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYC Near-atomic resolution cryo-EM reconstruction of peloruside-stabilized microtubule Deposited 2016-08-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 POU Peloruside A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYE Near-atomic resolution cryo-EM reconstruction of doubly bound Taxol- and peloruside-stabilized microtubule Deposited 2016-08-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 52 PDB declaration: 156-meric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 26 MG MAGNESIUM ION × 26 GDP GUANOSINE-5'-DIPHOSPHATE × 26 TA1 TAXOL × 26 POU Peloruside A × 26 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYE Near-atomic resolution cryo-EM reconstruction of doubly bound Taxol- and peloruside-stabilized microtubule Deposited 2016-08-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: hexameric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 POU Peloruside A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYE Near-atomic resolution cryo-EM reconstruction of doubly bound Taxol- and peloruside-stabilized microtubule Deposited 2016-08-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: hexameric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 POU Peloruside A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYF High-resolution cryo-EM reconstruction of Taxol-stabilized microtubule Deposited 2016-08-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 52 PDB declaration: 130-meric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 26 MG MAGNESIUM ION × 26 GDP GUANOSINE-5'-DIPHOSPHATE × 26 TA1 TAXOL × 26 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYF High-resolution cryo-EM reconstruction of Taxol-stabilized microtubule Deposited 2016-08-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: pentameric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYF High-resolution cryo-EM reconstruction of Taxol-stabilized microtubule Deposited 2016-08-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: pentameric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYG Cryo-EM reconstruction of zampanolide-bound microtubule Deposited 2016-08-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 52 PDB declaration: 130-meric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 26 MG MAGNESIUM ION × 26 GDP GUANOSINE-5'-DIPHOSPHATE × 26 ZPN (2Z,4E)-N-[(S)-[(1S,2E,5S,8E,10Z,17S)-3,11-dimethyl-19-methylidene-7,13-dioxo-6,21-dioxabicyclo[15.3.1]henicosa-2,8,10-trien-5-yl](hydroxy)methyl]hexa-2,4-dienamide × 26 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYG Cryo-EM reconstruction of zampanolide-bound microtubule Deposited 2016-08-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: pentameric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ZPN (2Z,4E)-N-[(S)-[(1S,2E,5S,8E,10Z,17S)-3,11-dimethyl-19-methylidene-7,13-dioxo-6,21-dioxabicyclo[15.3.1]henicosa-2,8,10-trien-5-yl](hydroxy)methyl]hexa-2,4-dienamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYG Cryo-EM reconstruction of zampanolide-bound microtubule Deposited 2016-08-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: pentameric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ZPN (2Z,4E)-N-[(S)-[(1S,2E,5S,8E,10Z,17S)-3,11-dimethyl-19-methylidene-7,13-dioxo-6,21-dioxabicyclo[15.3.1]henicosa-2,8,10-trien-5-yl](hydroxy)methyl]hexa-2,4-dienamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5XXT GDP-microtubule complexed with nucleotide-free KIF5C Deposited 2017-07-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain B 2–427（426 aa） Fragment：UNP residues 2-427
Chain D 2–427（426 aa） Fragment：UNP residues 2-427
Chain F 2–427（426 aa） Fragment：UNP residues 2-427
Chain H 2–427（426 aa） Fragment：UNP residues 2-427
Chain J 2–427（426 aa） Fragment：UNP residues 2-427
Chain L 2–427（426 aa） Fragment：UNP residues 2-427
Chain N 2–427（426 aa） Fragment：UNP residues 2-427
Chain P 2–427（426 aa） Fragment：UNP residues 2-427
Chain R 2–427（426 aa） Fragment：UNP residues 2-427
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.35 Å
5XXV GDP-microtubule complexed with KIF5C in AMPPNP state Deposited 2017-07-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain B 2–427（426 aa） Fragment：UNP residues 2-427
Chain D 2–427（426 aa） Fragment：UNP residues 2-427
Chain F 2–427（426 aa） Fragment：UNP residues 2-427
Chain H 2–427（426 aa） Fragment：UNP residues 2-427
Chain J 2–427（426 aa） Fragment：UNP residues 2-427
Chain L 2–427（426 aa） Fragment：UNP residues 2-427
Chain N 2–427（426 aa） Fragment：UNP residues 2-427
Chain P 2–427（426 aa） Fragment：UNP residues 2-427
Chain R 2–427（426 aa） Fragment：UNP residues 2-427
Not recorded MG MAGNESIUM ION × 9 GTP GUANOSINE-5'-TRIPHOSPHATE × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.46 Å
5XXW GDP-microtubule complexed with KIF5C in ATP state Deposited 2017-07-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain B 2–427（426 aa） Fragment：UNP residues 2-427
Chain D 2–427（426 aa） Fragment：UNP residues 2-427
Chain F 2–427（426 aa） Fragment：UNP residues 2-427
Chain H 2–427（426 aa） Fragment：UNP residues 2-427
Chain J 2–427（426 aa） Fragment：UNP residues 2-427
Chain L 2–427（426 aa） Fragment：UNP residues 2-427
Chain N 2–427（426 aa） Fragment：UNP residues 2-427
Chain P 2–427（426 aa） Fragment：UNP residues 2-427
Chain R 2–427（426 aa） Fragment：UNP residues 2-427
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.00 Å
5XXX GMPCPP-microtubule complexed with nucleotide-free KIF5C Deposited 2017-07-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain B 2–427（426 aa） Fragment：UNP residues 2-427
Chain D 2–427（426 aa） Fragment：UNP residues 2-427
Chain F 2–427（426 aa） Fragment：UNP residues 2-427
Chain H 2–427（426 aa） Fragment：UNP residues 2-427
Chain J 2–427（426 aa） Fragment：UNP residues 2-427
Chain L 2–427（426 aa） Fragment：UNP residues 2-427
Chain N 2–427（426 aa） Fragment：UNP residues 2-427
Chain P 2–427（426 aa） Fragment：UNP residues 2-427
Chain R 2–427（426 aa） Fragment：UNP residues 2-427
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 18 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.43 Å
6BJC TPX2_mini decorated GMPCPP-microtubule Deposited 2017-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 12 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80
cryo-EM vitrification conditions Cryogen ETHANE；blot for 4 seconds before plunging
Resolution 3.30 Å
6CVJ Model of synthetic tau (four tandem repeats of first repeat sequence) bound to the microtubule Deposited 2018-03-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 120 PDB declaration: 120-meric
Chain B 1–445（445 aa）
Chain C 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 30 MG MAGNESIUM ION × 30 GDP GUANOSINE-5'-DIPHOSPHATE × 60 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash
Resolution 3.20 Å
6CVJ Model of synthetic tau (four tandem repeats of first repeat sequence) bound to the microtubule Deposited 2018-03-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Chain C 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash
Resolution 3.20 Å
6CVJ Model of synthetic tau (four tandem repeats of first repeat sequence) bound to the microtubule Deposited 2018-03-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Chain C 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash
Resolution 3.20 Å
6CVN Model of synthetic tau (R2x4) bound to the microtubule Deposited 2018-03-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 120 PDB declaration: 120-meric
Chain A 1–445（445 aa）
Chain C 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 60 GTP GUANOSINE-5'-TRIPHOSPHATE × 30 MG MAGNESIUM ION × 30 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash.
Resolution 3.90 Å
6CVN Model of synthetic tau (R2x4) bound to the microtubule Deposited 2018-03-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–445（445 aa）
Chain C 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash.
Resolution 3.90 Å
6CVN Model of synthetic tau (R2x4) bound to the microtubule Deposited 2018-03-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–445（445 aa）
Chain C 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash.
Resolution 3.90 Å
6DPU Undecorated GMPCPP microtubule Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 12 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 buffer (80 mM PIPES, pH 6.8, 1 mM EGTA, 1 mM MgCl2) supplemented with 1 mM GMPCPP, 1 mM DTT, and 0.05% Nonident P-40
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.
Resolution 3.10 Å
6DPV Undecorated GDP microtubule Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 buffer (80 mM PIPES pH 6.8, 1 mM EGTA, 1 mM MgCl2) supplemented with 1 mM GTP, 1 mM DTT, and 0.05% Nonident P-40
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.
Resolution 3.30 Å
6DPW Undecorated GTPgammaS microtubule Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；Modified BRB80 buffer (80 mM PIPES, pH 6.8, 1 mM EGTA, 0.2 mM MgCl2) supplemented with 1 mM GTPgammaS, 1 mM DTT, and 0.05% Nonident P-40
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.
Resolution 3.50 Å
6EVW Cryo-EM structure of GMPCPP-microtubule co-polymerised with doublecortin Deposited 2017-11-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–429（429 aa）
Chain D 1–429（429 aa）
Chain F 1–429（429 aa）
Chain G 1–429（429 aa）
Chain H 1–429（429 aa）
Chain I 1–429（429 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 12 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
6EVX Cryo-EM structure of GDP.Pi-microtubule rapidly co-polymerised with doublecortin Deposited 2017-11-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 PO4 PHOSPHATE ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
6EVY Cryo-EM structure of GTPgammaS-microtubule co-polymerised with doublecortin Deposited 2017-11-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
6EVZ Cryo-EM structure of GDP-microtubule co-polymerised with doublecortin Deposited 2017-11-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6EW0 Cryo-EM structure of GDP-microtubule co-polymerised with doublecortin and supplemented with Taxol Deposited 2017-11-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 TA1 TAXOL × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6KIO Complex of yeast cytoplasmic dynein MTBD-High and MT without DTT Deposited 2019-07-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain b 2–427（426 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.94 Å
6KIQ Complex of yeast cytoplasmic dynein MTBD-High and MT with DTT Deposited 2019-07-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain b 2–427（426 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；DTT was added to the final concentration of 1 mM.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.62 Å
6LS4 A novel anti-tumor agent S-40 in complex with tubulin Deposited 2020-01-17 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 GOL GLYCEROL × 1 S40 3-[(4-cyclopropylphenyl)sulfonylamino]-4-methyl-N-(pyridin-3-ylmethyl)benzamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；6% PEG 4000, 6% GLYCEROL, 30mM MAGNESIUM CHLORIDE, 30mM CALCIUM CHLORIDE, 3.3% Jeffamin M-600, 100mM MES
Resolution 2.40 Å R-free 0.240
6MLQ Cryo-EM structure of microtubule-bound Kif7 in the ADP state Deposited 2018-09-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blotted from behind the grid for 2 seconds
Resolution 4.20 Å
6MLR Cryo-EM structure of microtubule-bound Kif7 in the AMPPNP state Deposited 2018-09-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blotted from behind the grid for 2 seconds
Resolution 4.20 Å
6MZE Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain I 1–445（445 aa）
Chain K 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
Resolution 3.60 Å R-free 0.245
6MZE Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain P 1–445（445 aa）
Chain R 1–445（445 aa）
Chain W 1–445（445 aa）
Chain Y 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
Resolution 3.60 Å R-free 0.245
6MZF Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain I 1–445（445 aa）
Chain K 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
Resolution 4.40 Å R-free 0.252
6MZF Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain P 1–445（445 aa）
Chain R 1–445（445 aa）
Chain W 1–445（445 aa）
Chain Y 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
Resolution 4.40 Å R-free 0.252
6MZG Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
Resolution 3.21 Å R-free 0.234
6MZG Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain H 1–445（445 aa）
Chain J 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
Resolution 3.21 Å R-free 0.234
6O2Q Acetylated Microtubules Deposited 2019-02-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；Contains 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH (stored at 4 degrees Celsius).
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 4 seconds at blot force 10.
Resolution 3.70 Å
6O2R Deacetylated Microtubules Deposited 2019-02-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；Contains 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH (stored at 4 degrees Celsius).
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 4 seconds at blot force 10.
Resolution 3.30 Å
6O2S Deacetylated Microtubules Deposited 2019-02-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 104 PDB declaration: 104-meric
Chain 1H 1–445（445 aa）
Chain 1O 1–445（445 aa）
Chain 1P 1–445（445 aa）
Chain 1Q 1–445（445 aa）
Chain 1R 1–445（445 aa）
Chain 1S 1–445（445 aa）
Chain 1T 1–445（445 aa）
Chain 1U 1–445（445 aa）
Chain 1V 1–445（445 aa）
Chain 1W 1–445（445 aa）
Chain 1X 1–445（445 aa）
Chain 1Y 1–445（445 aa）
Chain 1Z 1–445（445 aa）
Chain 2H 1–445（445 aa）
Chain 2O 1–445（445 aa）
Chain 2P 1–445（445 aa）
Chain 2Q 1–445（445 aa）
Chain 2R 1–445（445 aa）
Chain 2S 1–445（445 aa）
Chain 2T 1–445（445 aa）
Chain 2U 1–445（445 aa）
Chain 2V 1–445（445 aa）
Chain 2W 1–445（445 aa）
Chain 2X 1–445（445 aa）
Chain 2Y 1–445（445 aa）
Chain 2Z 1–445（445 aa）
Chain 3H 1–445（445 aa）
Chain 3O 1–445（445 aa）
Chain 3P 1–445（445 aa）
Chain 3Q 1–445（445 aa）
Chain 3R 1–445（445 aa）
Chain 3S 1–445（445 aa）
Chain 3T 1–445（445 aa）
Chain 3U 1–445（445 aa）
Chain 3V 1–445（445 aa）
Chain 3W 1–445（445 aa）
Chain 3X 1–445（445 aa）
Chain 3Y 1–445（445 aa）
Chain 3Z 1–445（445 aa）
Chain 4H 1–445（445 aa）
Chain 4O 1–445（445 aa）
Chain 4P 1–445（445 aa）
Chain 4Q 1–445（445 aa）
Chain 4R 1–445（445 aa）
Chain 4S 1–445（445 aa）
Chain 4T 1–445（445 aa）
Chain 4U 1–445（445 aa）
Chain 4V 1–445（445 aa）
Chain 4W 1–445（445 aa）
Chain 4X 1–445（445 aa）
Chain 4Y 1–445（445 aa）
Chain 4Z 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 52 MG MAGNESIUM ION × 52 GDP GUANOSINE-5'-DIPHOSPHATE × 52 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；Contains 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH (stored at 4 degrees Celsius).
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 4 seconds at blot force 10.
Resolution 4.00 Å
6O2T Acetylated Microtubules Deposited 2019-02-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 104 PDB declaration: 104-meric
Chain 1H 1–445（445 aa）
Chain 1O 1–445（445 aa）
Chain 1P 1–445（445 aa）
Chain 1Q 1–445（445 aa）
Chain 1R 1–445（445 aa）
Chain 1S 1–445（445 aa）
Chain 1T 1–445（445 aa）
Chain 1U 1–445（445 aa）
Chain 1V 1–445（445 aa）
Chain 1W 1–445（445 aa）
Chain 1X 1–445（445 aa）
Chain 1Y 1–445（445 aa）
Chain 1Z 1–445（445 aa）
Chain 2H 1–445（445 aa）
Chain 2O 1–445（445 aa）
Chain 2P 1–445（445 aa）
Chain 2Q 1–445（445 aa）
Chain 2R 1–445（445 aa）
Chain 2S 1–445（445 aa）
Chain 2T 1–445（445 aa）
Chain 2U 1–445（445 aa）
Chain 2V 1–445（445 aa）
Chain 2W 1–445（445 aa）
Chain 2X 1–445（445 aa）
Chain 2Y 1–445（445 aa）
Chain 2Z 1–445（445 aa）
Chain 3H 1–445（445 aa）
Chain 3O 1–445（445 aa）
Chain 3P 1–445（445 aa）
Chain 3Q 1–445（445 aa）
Chain 3R 1–445（445 aa）
Chain 3S 1–445（445 aa）
Chain 3T 1–445（445 aa）
Chain 3U 1–445（445 aa）
Chain 3V 1–445（445 aa）
Chain 3W 1–445（445 aa）
Chain 3X 1–445（445 aa）
Chain 3Y 1–445（445 aa）
Chain 3Z 1–445（445 aa）
Chain 4H 1–445（445 aa）
Chain 4O 1–445（445 aa）
Chain 4P 1–445（445 aa）
Chain 4Q 1–445（445 aa）
Chain 4R 1–445（445 aa）
Chain 4S 1–445（445 aa）
Chain 4T 1–445（445 aa）
Chain 4U 1–445（445 aa）
Chain 4V 1–445（445 aa）
Chain 4W 1–445（445 aa）
Chain 4X 1–445（445 aa）
Chain 4Y 1–445（445 aa）
Chain 4Z 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 52 MG MAGNESIUM ION × 52 GDP GUANOSINE-5'-DIPHOSPHATE × 52 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；Contains 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH (stored at 4 degrees Celsius).
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 4 seconds at blot force 10.
Resolution 4.10 Å
6RZA Cryo-EM structure of the human inner arm dynein DNAH7 microtubule binding domain bound to microtubules Deposited 2019-06-13 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–426（426 aa）
Chain D 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
6RZB Cryo-EM structure of mouse cytoplasmic dynein-1 microtubule binding domain bound to microtubules Deposited 2019-06-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
6TA3 Human kinesin-5 motor domain in the GSK-1 state bound to microtubules (Conformation 1) Deposited 2019-10-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–429（429 aa）
Not recorded G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 MG MAGNESIUM ION × 2 MZK 6-[4-(trifluoromethyl)phenyl]-3,4-dihydro-1~{H}-quinolin-2-one × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6TA4 Human kinesin-5 motor domain in the AMPPNP state bound to microtubules Deposited 2019-10-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–429（429 aa）
Not recorded G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.10 Å
6TIW Human kinesin-5 motor domain in the GSK state bound to microtubules (Conformation 2) Deposited 2019-11-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–429（429 aa）
Not recorded MZK 6-[4-(trifluoromethyl)phenyl]-3,4-dihydro-1~{H}-quinolin-2-one × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6VPO Cryo-EM structure of microtubule-bound KLP61F motor domain in the AMPPNP state Deposited 2020-02-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
6VPP Cryo-EM structure of microtubule-bound KLP61F motor with tail domain in the nucleotide-free state Deposited 2020-02-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
6Y4M Structure of Tubulin Tyrosine Ligase in Complex with Tb111 Deposited 2020-02-21 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 O9B (2~{R})-1-methylpiperidine-2-carboxylic acid × 1 O9H [(1~{R},3~{R})-1-acetyloxy-1-[4-methanoyl-5-(2-phenylmethoxyethyl)-2,3-dihydro-1,3-thiazol-2-yl]-4-methyl-pentan-3-yl]-methyl-azanium × 1 OH5 (2~{S},4~{R})-4-azanyl-2-methyl-5-phenyl-pentanoic acid × 1 PGE TRIETHYLENE GLYCOL × 1 VAL VALINE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.03 M calcium chloride, 0.03 M magnesium chloride, 0.10 M MES pH 6.50, 3.20% w/v PEG 4000, 5.00% w/v glycerol
Resolution 3.34 Å R-free 0.234
6Y4N Structure of Tubulin Tyrosine Ligase in Complex with Tb116 Deposited 2020-02-21 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 O9B (2~{R})-1-methylpiperidine-2-carboxylic acid × 1 O9K [(1~{R},3~{R})-1-(4-methanoyl-1,3-thiazol-2-yl)-4-methyl-3-(methylamino)pentyl] ethanoate × 1 O9N methyl (2~{S},4~{S})-2,4-bis(azanyl)-5-phenyl-pentanoate × 1 PGE TRIETHYLENE GLYCOL × 1 VAL VALINE × 1 P6S benzyl hydrogen carbonate × 1 PEG DI(HYDROXYETHYL)ETHER × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；30 mM calcium chloride, 30 mM magnesium chloride, 0.10 M MES pH 6.0, 2.0% w/v PEG 4000, 5.0% w/v glycerol
Resolution 2.85 Å R-free 0.220
6ZPI Microtubule complexed with Kif15 motor domain. Symmetrised asymmetric unit Deposited 2020-07-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–431（431 aa）
Not recorded ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
7CBZ Crystal structure of T2R-TTL-A31 complex Deposited 2020-06-15 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 FUO 2-[5-[4-[2-[4-(2-cyclopropylethanoyl)piperazin-1-yl]ethoxy]phenyl]pyridin-2-yl]-N-(phenylmethyl)ethanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, and 30 mM MgCl2.
Resolution 2.61 Å R-free 0.255
7DMZ GMPCPP microtubule complex Deposited 2020-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–445（445 aa）
Chain E 1–445（445 aa）
Chain F 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 2 TAJ taccalonolide AJ × 3 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
7DN0 AJ-GMPCPP-MT-non-seam Deposited 2020-12-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 1–445（445 aa）
Chain F 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 4 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 TAJ taccalonolide AJ × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
7EMJ Crystal structure of T2R-TTL-Barbigerone complex Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 J6L 8,8-dimethyl-3-(2,4,5-trimethoxyphenyl)pyrano[2,3-f]chromen-4-one × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1 M MES, 30 mM CaCl2 and 30 mM MgCl2, pH 6.7
Resolution 2.33 Å R-free 0.241
7EXC Crystal structure of T2R-TTL-1129A2 complex Deposited 2021-05-26 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 JEL N-[[3-(1,3-benzodioxol-5-yloxy)phenyl]methyl]-9H-pyrido[3,4-b]indol-3-amine × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, 30 mM MgCl2
Resolution 2.39 Å R-free 0.264
7L05 Complex of novel maytansinoid M24 bound to T2R-TTL (two tubulin alpha/beta heterodimers, RB3 stathmin-like domain, and tubulin tyrosine ligase) Deposited 2020-12-11 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 5 CA CALCIUM ION × 4 CL CHLORIDE ION × 1 EDO 1,2-ETHANEDIOL × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 XQ4 (1S,2R,3S,5S,6S,16E,18E,20R,21S)-11-chloro-21-hydroxy-12,20-dimethoxy-2,5,9,16-tetramethyl-8,23-dioxo-4,24-dioxa-9,22-diazatetracyclo[19.3.1.1~10,14~.0~3,5~]hexacosa-10(26),11,13,16,18-pentaen-6-yl (2S)-2-{methyl[3-(methylamino)propanoyl]amino}propanoate (non-preferred name) × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；PEG 3350, MES, CaCl2, MgCl2, glycerol
Resolution 2.21 Å R-free 0.217
7NB8 Plasmodium falciparum kinesin-5 motor domain without nucleotide, complexed with 14 protofilament microtubule. Deposited 2021-01-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 uM microtubules were incubated to the grid and incubated for 30 seconds. The grid was blotted, then 40 uM kinesin motor domain added and incubated for 30 seconds. The grid was again blotted and 40 uM kinesin motor domain added for 30 seconds. Then the grid was blotted and plunge frozen.
Resolution 4.40 Å
7NBA Plasmodium falciparum kinesin-5 motor domain bound to AMPPNP, complexed with 14 protofilament microtubule. Deposited 2021-01-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；5 uM microtubules were applied to the grid and incubated for 30 seconds. The grid was blotted, then 40 uM kinesin motor domain added, incubated for 30 seconds, then blotted. 40 uM Kinesin motor domain was again added, incubated for 40 seconds, blotted, then plunge frozen.
Resolution 4.00 Å
7PQC tau-microtubule structural ensemble based on CryoEM data Deposited 2021-09-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain A 1–445（445 aa）
Chain C 1–445（445 aa）
Chain E 1–445（445 aa）
Chain G 1–445（445 aa）
Chain I 1–445（445 aa）
Chain K 1–445（445 aa）
Chain M 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 7 MG MAGNESIUM ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7PQP tau-microtubule structural ensemble based on CryoEM data Deposited 2021-09-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain A 1–445（445 aa）
Chain C 1–445（445 aa）
Chain E 1–445（445 aa）
Chain G 1–445（445 aa）
Chain I 1–445（445 aa）
Chain K 1–445（445 aa）
Chain M 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 7 MG MAGNESIUM ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7RS5 Cryo-EM structure of Kip3 (AMPPNP) bound to Taxol-Stabilized Microtubules Deposited 2021-08-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain H 1–445（445 aa）
Chain J 1–445（445 aa）
Chain M 1–445（445 aa）
Chain O 1–445（445 aa）
Chain Q 1–445（445 aa）
Chain S 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 18 GDP GUANOSINE-5'-DIPHOSPHATE × 9 TA1 TAXOL × 9 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；cryoEM buffer (50 mM Tris-HCl, pH 8.0, 1 mM MgCl2, 1 mM EGTA, 1 mM DTT supplemented with 2mM AMPPNP)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
7RS6 Cryo-EM structure of Kip3 (AMPPNP) bound to GMPCPP-Stabilized Microtubules Deposited 2021-08-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain H 1–445（445 aa）
Chain J 1–445（445 aa）
Chain M 1–445（445 aa）
Chain O 1–445（445 aa）
Chain Q 1–445（445 aa）
Chain S 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 27 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 9 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 buffer (80 mM PIPES-KOH, pH 6.8; 1 mM MgCl2, 1 mM EGTA, 1 mM DTT)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7RX0 Complex of AMPPNP-Kif7 and Gli2 Zinc-Finger domain bound to microtubules Deposited 2021-08-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Not recorded ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blotted from behind the grid for 2 seconds
Resolution 3.89 Å
7SGS Cryo-EM structure of full-length MAP7 bound to the microtubule Deposited 2021-10-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain C 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7U0F HIV-1 Rev in complex with tubulin Deposited 2022-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 150 PDB declaration: 150-meric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.53 Å
7U0F HIV-1 Rev in complex with tubulin Deposited 2022-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.53 Å
7U0F HIV-1 Rev in complex with tubulin Deposited 2022-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.53 Å
7X4N Crystal Structure of C. elegans kinesin-4 KLP-12 complexed with tubulin and DARPin Deposited 2022-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；ammonium acetate, HEPES, polyethylene glycol (PEG) 3350
Resolution 2.88 Å R-free 0.296
7YHN ANTI-TUMOR AGENT Y48 IN COMPLEX WITH TUBULIN Deposited 2022-07-14 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 IUK 4-methyl-3-[(4-methylphenyl)sulfonylamino]-~{N}-[(6-methylpyridin-3-yl)methyl]benzamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；6% PEG 4000, 8% GLYCEROL, 30MM MAGNESIUM CHLORIDE, 30MM CALCIUM CHLORIDE, 100MM MES
Resolution 2.60 Å R-free 0.272
7YSN Tubulin heterodimer structure of GMPCPP state in solution Deposited 2022-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
7YSO Tubulin heterodimer structure of GDP-1 state in solution Deposited 2022-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7YSP Tubulin heterodimer structure of GDP-2 state in solution Deposited 2022-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
7Z2A P. berghei kinesin-8B motor domain in no nucleotide state bound to tubulin dimer Deposited 2022-02-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain H 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
7Z2B P. berghei kinesin-8B motor domain in AMPPNP state bound to tubulin dimer Deposited 2022-02-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain H 1–426（426 aa）
Not recorded ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7Z2C P. falciparum kinesin-8B motor domain in no nucleotide bound to tubulin dimer Deposited 2022-02-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain H 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8JJB Crystal structure of T2R-TTL-Y61 complex Deposited 2023-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–431（431 aa）
Chain D 1–431（431 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 USI ~{N}4-(1~{H}-indol-5-ylmethyl)-6-(3-methoxyphenyl)pyrimidine-2,4-diamine × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 CL CHLORIDE ION × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, 30 mM MgCl2
Resolution 2.68 Å R-free 0.260
8JJC Tubulin-Y62 Deposited 2023-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–431（431 aa）
Chain D 1–431（431 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 UPO 4-(6,7-dimethoxy-3,4-dihydro-1~{H}-isoquinolin-2-yl)-6-(3-methoxyphenyl)pyrimidin-2-amine × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 CL CHLORIDE ION × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, 30 mM MgCl2
Resolution 2.76 Å R-free 0.264
8QAU Outer kinetochore Ndc80-Dam1 alpha/beta-tubulin complex Deposited 2023-08-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
8RC1 MAP7 MTBD (microtubule binding domain) decorated microtubule protofilament Deposited 2023-12-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–430（430 aa）
Chain D 1–430（430 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；80mM PIPES, 2mM MgCl2, 1mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE；Microtubules stabilised by MAP7 MTBD were applied to a glow discharged grid, incubated for 30 seconds at room temperature following which excess sample was wicked off and MAP7 MTBD was immediately added to the grid. After incubation for 60 seconds in a Vitrobot, excess liquid was blotted off and the grid was plunge frozen in liquid ethane.
Resolution 3.70 Å
8RHB Microtubule-associated kinesin-1 tail complex bound to AMPPNP, single-headed state Deposited 2023-12-15 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 + 5mM AMPPNP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RHH Microtubule-associated kinesin-1 tail complex bound to AMPPNP, two-headed state Deposited 2023-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: pentameric
Chain B 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 + 5mM AMPPNP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RIK Microtubule-associated kinesin-1 tail complex bound to ADP, single-headed state Deposited 2023-12-18 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 + 5 mM ADP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8RIZ Microtubule-associated kinesin-1 tail complex bound to ADP, two-headed state Deposited 2023-12-19 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 + 5mM ADP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8V4K CCP5 in complex with microtubules class1 Deposited 2023-11-29 Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ZN ZINC ION × 1 GLU GLUTAMIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8V4L CCP5 in complex with microtubules class2 Deposited 2023-11-29 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 GLU GLUTAMIC ACID × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8V4M CCP5 in complex with microtubules class3 Deposited 2023-11-29 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 GLU GLUTAMIC ACID × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8X9P HURP (428-534)-alpha-tubulin-beta-tubulin complex Deposited 2023-11-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–427（427 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
9DA8 Tau-Microtubule structure in the presence of ATP Deposited 2024-08-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–445（445 aa）
Chain C 1–445（445 aa）
Chain K 1–445（445 aa）
Chain M 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 4 TA1 TAXOL × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.94 Å
9DI0 Cryo-EM structure of Kif18A bound to a microtubule Deposited 2024-09-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric
Chain K 1–445（445 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 2 TA1 TAXOL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9DUQ HURP(65-174) bound to GMPCPP-stabilized microtubule Deposited 2024-10-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric
Chain B 1–427（427 aa）
Chain D 1–427（427 aa）
Chain F 1–427（427 aa）
Chain H 1–427（427 aa）
Chain J 1–427（427 aa）
Chain L 1–427（427 aa）
Chain N 1–427（427 aa）
Chain P 1–427（427 aa）
Chain R 1–427（427 aa）
Not recorded G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 9 MG MAGNESIUM ION × 18 GTP GUANOSINE-5'-TRIPHOSPHATE × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 (1X): 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9DXC Model of tubulin dimers used for determining the dimer rise in a taxol-stabilized microtubule-HURP complex Deposited 2024-10-11 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9DXE Model of tubulin dimers used for determining the dimer rise in a taxol-stabilized microtubule Deposited 2024-10-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9EDR Tubulin Cofactors D,E,G,C and Tubulin complex -- TBCC N Terminus Bound to Tubulin Deposited 2024-11-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
9EDS Tubulin cofactors D,E,G,C bound to tubulin dimer -- TBCC N terminus unbound Deposited 2024-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
9EDT Tubulin cofactors D,E,G bound to tubulin dimer Deposited 2024-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9EEB Tubulin cofactors D,E,G bound to tubulin dimer Deposited 2024-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9GNQ Microtubule-associated Kif5B IAK tail mutant bound to ADP Deposited 2024-09-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 + 5mM AMPPNP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9IM5 Tubulin-RB3(MUT)-TTL-Y12 Deposited 2024-07-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 CL CHLORIDE ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 A1L2T ~{N}4-(1,3-benzodioxol-5-ylmethyl)-6-(1~{H}-indol-4-yl)pyrimidine-2,4-diamine × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1M MES, 30mM CaCl2, 30mM MgCl2, pH 6.7
Resolution 2.86 Å R-free 0.246
9IMO Crystal structure of Tubulin-RB3-TTL-Y12 Deposited 2024-07-04 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–431（431 aa）
Chain D 1–431（431 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 5 CA CALCIUM ION × 3 A1L2T ~{N}4-(1,3-benzodioxol-5-ylmethyl)-6-(1~{H}-indol-4-yl)pyrimidine-2,4-diamine × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1M MES, 30mM CaCl2, 30mM MgCl2, pH 6.7
Resolution 2.75 Å R-free 0.255
9KBW CryoEM structure of microtubule bound with GAS2-GAR domain. Deposited 2024-10-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–426（426 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；80 mM PIPES, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9L7M Nucleotide-free kinesin-1 motor domain bound to the microtubule Deposited 2024-12-26 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
9N9E Cryo-EM structure of the SAH domain of Caenorhabditis elegans ICP-1 bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
9N9F Cryo-EM structure of the SAH domain of human INCENP bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.91 Å
9N9G Cryo-EM structure of the human Hec1-Nuf2 dimer bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 1–445（445 aa）
Chain b 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9N9H Cryo-EM structure of the Xenopus laevis mitotic centromere-associated kinesin (MCAK) bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain b 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.31 Å
9N9I Cryo-EM structure of the Xenopus laevis mitotic centromere-associated kinesin (MCAK) bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain 3 1–445（445 aa）
Chain B 1–445（445 aa）
Chain C 1–445（445 aa）
Chain b 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 TA1 TAXOL × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9NNL Bordetella filamentous hemagglutinin (FhaB) C-terminal domain bound to microtubules Deposited 2025-03-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain H 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 4 TA1 TAXOL × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.42 Å
9RPD D. melanogaster Augmin TII N-clamp (GST-fusion) bound to a microtubule, well-defined subset of particles Deposited 2025-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.90 Å
9T1D Cryo-EM reconstruction of undecorated GDP microtubule Deposited 2025-10-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–429（429 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
9U6A Tubulin-DARPin D1 in complex with a flavone Deposited 2025-03-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–431（431 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 A1L8X 3-methoxy-2-(4-methoxy-3-oxidanyl-phenyl)-5,7-bis(oxidanyl)chromen-4-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；PEG 3000, ammonium sulfate, bis-tris methane
Resolution 1.92 Å R-free 0.224
9U6D Crystal structure of tubulin-RB3-TTL in complex with X8 Deposited 2025-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–431（431 aa）
Chain D 1–431（431 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 A1L8Z 2-[2-fluoranyl-4-[4-[2-[4-[(1~{R},2~{S})-2-fluoranylcyclopropyl]carbonylpiperazin-1-yl]ethoxy]phenyl]phenyl]-~{N}-(phenylmethyl)ethanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1M MES, 30mM CaCl2, 30mM MgCl2, pH 6.7
Resolution 2.54 Å R-free 0.238
9U9Q Tubulin-RB3-TTL in complex with C38 Deposited 2025-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–431（431 aa）
Chain D 1–431（431 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 7 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 A1L80 2-(1-benzofuran-5-yl)-6-[2,6-bis(fluoranyl)-4-[3-(methylamino)propoxy]phenyl]-5-chloranyl-~{N}-[(2~{S})-1,1,1-tris(fluoranyl)propan-2-yl]-[1,2,4]triazolo[1,5-a]pyrimidin-7-amine × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1M MES, 30mM CaCl2, 30mM MgCl2, pH 6.7
Resolution 2.93 Å R-free 0.269
9YMG Human KIF18A-DARPin fusion protein bound to AMP-PNP and tubulin Deposited 2025-10-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–444（444 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 EDO 1,2-ETHANEDIOL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.2 M ammonium nitrate, 0.1 M sodium acetate pH 5.6, 20% (w/v) PEG Smear Low (5% PEG 400, 5% PEG 500 MME, 5% PEG 600, 5% PEG 1000), 5% (v/v) ethylene glycol
Resolution 2.41 Å R-free 0.238
9YMG Human KIF18A-DARPin fusion protein bound to AMP-PNP and tubulin Deposited 2025-10-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain E 1–444（444 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.2 M ammonium nitrate, 0.1 M sodium acetate pH 5.6, 20% (w/v) PEG Smear Low (5% PEG 400, 5% PEG 500 MME, 5% PEG 600, 5% PEG 1000), 5% (v/v) ethylene glycol
Resolution 2.41 Å R-free 0.238
9YXQ Cryo-EM structure of the Xenopus laevis mitotic centromere-associated kinesin (MCAK) bound to the paclitaxel-stabilized microtubule Deposited 2025-10-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: 14-meric
Chain 2 1–445（445 aa）
Chain 4 1–445（445 aa）
Chain 5 1–445（445 aa）
Chain 7 1–445（445 aa）
Chain B 1–445（445 aa）
Chain b 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 11 GTP GUANOSINE-5'-TRIPHOSPHATE × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 TA1 TAXOL × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.39 Å

137 other PDB entries and 159 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name TBB_PIG
Isoform —
PDB entities 2
Chains and sequence ranges Author chain A； PDBConstruct 1–445； UniProt 1–445 Author chain K； PDBConstruct 1–445； UniProt 1–445

Tubulin alpha-1B chain

OrganismNot specified

UniProt Q2XVP4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 5 PDB declaration: pentameric（5） Consistent with protein copy count Chain B； UniProt 1–451 Chain L； UniProt 1–451 Not recorded Disks large-associated protein 5 × 1 （Q15398） Tubulin beta chain × 2 （P02554） TA1 TAXOL × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 6.9 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.10 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9DHZ Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3J8X High-resolution structure of no-nucleotide kinesin on microtubules Deposited 2014-11-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 25 mM PIPES, 25 mM NaCl, 2 mM MgCl2, 1 mM EGTA；pH 6.8；25 mM PIPES, 25 mM NaCl, 2 mM MgCl2, 1 mM EGTA
cryo-EM vitrification conditions No glow discharge was applied. After sample application to grid, liquid was mostly 'wicked' away by edgewise application of filter paper. Subsequently, blotting and plunge freezing were performed with ~0.5 second delay after blotting but prior to plunging.；Cryogen ETHANE；No glow discharge was applied. After sample application to grid, liquid was mostly 'wicked' away by edgewise application of filter paper. Subsequently, blotting and plunge freezing were performed with ~0.5 second delay after blotting but prior to plunging into liquid ethane.
Resolution 5.00 Å
3J8Y High-resolution structure of ATP analog-bound kinesin on microtubules Deposited 2014-11-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 25 mM PIPES, 25 mM NaCl, 2 mM MgCl2, 1 mM EGTA；pH 6.8；25 mM PIPES, 25 mM NaCl, 2 mM MgCl2, 1 mM EGTA
cryo-EM vitrification conditions No glow discharge was applied. After sample application to grid, liquid was mostly 'wicked' away by edgewise application of filter paper. Subsequently, blotting and plunge freezing were performed with ~0.5 second delay after blotting but prior to plunging.；Cryogen ETHANE；No glow discharge was applied. After sample application to grid, liquid was mostly 'wicked' away by edgewise application of filter paper. Subsequently, blotting and plunge freezing were performed with ~0.5 second delay after blotting but prior to plunging into liquid ethane.
Resolution 5.00 Å
3JAK Cryo-EM structure of GTPgammaS-microtubule co-polymerized with EB3 (merged dataset with and without kinesin bound) Deposited 2015-06-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain E 1–451（451 aa）
Chain J 1–451（451 aa）
Chain K 1–451（451 aa）
Chain L 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions Blot once for 4 seconds before plunging.；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 3.30 Å
3JAL Cryo-EM structure of GMPCPP-microtubule co-polymerized with EB3 Deposited 2015-06-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain E 1–451（451 aa）
Chain J 1–451（451 aa）
Chain K 1–451（451 aa）
Chain L 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 ELECTRON MICROSCOPY
cryo-EM buffer BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions Blot once for 4 seconds before plunging；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 3.50 Å
3JAR Cryo-EM structure of GDP-microtubule co-polymerized with EB3 Deposited 2015-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain E 1–451（451 aa）
Chain J 1–451（451 aa）
Chain K 1–451（451 aa）
Chain L 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions Blot once for 4 seconds before plunging/；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 3.40 Å
3JAS Cryo-EM structure of dynamic GDP-microtubule (14 protofilaments) decorated with kinesin Deposited 2015-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain E 1–451（451 aa）
Chain J 1–451（451 aa）
Chain K 1–451（451 aa）
Chain L 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions Blot once for 4 seconds before plunging.；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 3.50 Å
3JAT Cryo-EM structure of GMPCPP-microtubule (14 protofilaments) decorated with kinesin Deposited 2015-06-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain E 1–451（451 aa）
Chain J 1–451（451 aa）
Chain K 1–451（451 aa）
Chain L 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 12 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 ELECTRON MICROSCOPY
cryo-EM buffer BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions Blot once for 4 seconds before plunging.；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 3.50 Å
3JAW Atomic model of a microtubule seam based on a cryo-EM reconstruction of the EB3-bound microtubule (merged dataset containing tubulin bound to GTPgammaS, GMPCPP, and GDP) Deposited 2015-06-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions Blot once for 4 seconds before plunging.；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 3.90 Å
4ZHQ Crystal structure of Tubulin-Stathmin-TTL-MMAE Complex Deposited 2015-04-26 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 GOL GLYCEROL × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 4Q5 N-methyl-L-valyl-N-[(3R,4S,5S)-1-{(2S)-2-[(1R,2R)-3-{[(1S,2R)-1-hydroxy-1-phenylpropan-2-yl]amino}-1-methoxy-2-methyl-3-oxopropyl]pyrrolidin-1-yl}-3-methoxy-5-methyl-1-oxoheptan-4-yl]-N-methyl-L-valinamide × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.7；293 K；6% PEG, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 2.55 Å R-free 0.245
4ZI7 CRYSTAL STRUCTURE OF TUBULIN-STATHMIN-TTL-HTI286 COMPLEX Deposited 2015-04-27 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 4 GOL GLYCEROL × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 4SL N,beta,beta-trimethyl-L-phenylalanyl-N-[(3S,4Z)-5-carboxy-2-methylhex-4-en-3-yl]-N,3-dimethyl-L-valinamide × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；293 K；PEG, glycerol, MES, CaCl2, MgCl2
Resolution 2.51 Å R-free 0.239
4ZOL Crystal Structure of Tubulin-Stathmin-TTL-Tubulysin M Complex Deposited 2015-05-06 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 55Q (2R,4R)-4-{[(2-{(1R,3R)-1-(acetyloxy)-4-methyl-3-[methyl(N-{[(2S)-1-methylpiperidin-2-yl]carbonyl}-D-isoleucyl)amino]pentyl}-1,3-thiazol-4-yl)carbonyl]amino}-2-methyl-5-phenylpentanoic acid × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.7；293 K；6% PEG, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 2.50 Å R-free 0.232
5CA0 Crystal structure of T2R-TTL-Lexibulin complex Deposited 2015-06-29 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 CA CALCIUM ION × 2 GOL GLYCEROL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 LXL 1-ethyl-3-[2-methoxy-4-(5-methyl-4-{[(1S)-1-(pyridin-3-yl)butyl]amino}pyrimidin-2-yl)phenyl]urea × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% polyethylene glycol 4000, 8% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.50 Å R-free 0.237
5EZY Crystal structure of T2R-TTL-taccalonolide AJ complex Deposited 2015-11-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 TAJ taccalonolide AJ × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% polyethylene glycol 4000, 8% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.05 Å R-free 0.208
5FNV a new complex structure of tubulin with an alpha-beta unsaturated lactone Deposited 2015-11-16 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X3H PIRONETIN × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.7；0.1M MES PH6.7;6% PEG4000 ;5% GLYCEROL;30MM CACL2;30MM MGCL2
Resolution 2.61 Å R-free 0.226
5H74 Crystal structure of T2R-TTL-14b complex Deposited 2016-11-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa） Fragment：UNP residues 1-450
Chain C 1–450（450 aa） Fragment：UNP residues 1-450
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 7LG (2~{S},4~{R})-4-[[2-[(1~{R},3~{R})-1-acetyloxy-3-[hexyl-[(2~{S},3~{S})-3-methyl-2-[[(2~{R})-1-methylpiperidin-2-yl]carbonylamino]pentanoyl]amino]-4-methyl-pentyl]-1,3-thiazol-4-yl]carbonylamino]-5-(4-fluorophenyl)-2-methyl-pentanoic acid × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.60 Å R-free 0.248
5H7O Crystal structure of DJ-101 in complex with tubulin protein Deposited 2016-11-20 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 7Q7 2-(1H-indol-4-yl)-4-(3,4,5-trimethoxyphenyl)-1H-imidazo[4,5-c]pyridine × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M Mes, 30 mM CaCl2 and 30 mM MgCl2 (pH 6.7)
Resolution 2.80 Å R-free 0.256
5JCB Microtubule depolymerizing agent podophyllotoxin derivative YJTSF1 Deposited 2016-04-14 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 7 GOL GLYCEROL × 8 IMD IMIDAZOLE × 2 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 NA SODIUM ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 NV4 (5R,5aR,8aS,9R)-9-[(4H-1,2,4-triazol-3-yl)sulfanyl]-5-(3,4,5-trimethoxyphenyl)-5,8,8a,9-tetrahydro-2H-furo[3',4':6,7]naphtho[2,3-d][1,3]dioxol-6(5aH)-one × 2 PEG DI(HYDROXYETHYL)ETHER × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；PEG 4000, Glycerol, Magnesium chloride, Calcium chloride
Resolution 2.30 Å R-free 0.200
5JQG An apo tubulin-RB-TTL complex structure used for side-by-side comparison Deposited 2016-05-04 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.7；293 K；0.1M MES, 6% PEG4000, 5% Glycerol, 30mM CaCl2, 30mM MgCl2
Resolution 2.24 Å R-free 0.212
5KMG Near-atomic cryo-EM structure of PRC1 bound to the microtubule Deposited 2016-06-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain A 1–441（441 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 14 MG MAGNESIUM ION × 14 GDP GUANOSINE-5'-DIPHOSPHATE × 14 POU Peloruside A × 14 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot for 4 seconds, blot force 10
Resolution 3.50 Å
5KMG Near-atomic cryo-EM structure of PRC1 bound to the microtubule Deposited 2016-06-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–441（441 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 POU Peloruside A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot for 4 seconds, blot force 10
Resolution 3.50 Å
5KMG Near-atomic cryo-EM structure of PRC1 bound to the microtubule Deposited 2016-06-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–441（441 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 POU Peloruside A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot for 4 seconds, blot force 10
Resolution 3.50 Å
5XIW Crystal structure of T2R-TTL-Colchicine complex Deposited 2017-04-27 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 LOC N-[(7S)-1,2,3,10-tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[d]heptalen-7-yl]ethanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% polyethylene glycol 4000, 8% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.90 Å R-free 0.253
5XKE Crystal structure of T2R-TTL-Demecolcine complex Deposited 2017-05-07 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 LON (7S)-1,2,3,10-tetramethoxy-7-(methylamino)-6,7-dihydro-5H-benzo[a]heptalen-9-one × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% polyethylene glycol 4000, 8% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.60 Å R-free 0.264
5XKF Crystal structure of T2R-TTL-MPC6827 complex Deposited 2017-05-07 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 88U N-(4-methoxyphenyl)-N,2-dimethyl-quinazolin-4-amine × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% polyethylene glycol 4000, 8% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.80 Å R-free 0.259
5XKG Crystal structure of T2R-TTL-CH1 complex Deposited 2017-05-07 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 890 4-[(3-azanyl-4-methoxy-phenyl)-methyl-amino]chromen-2-one × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% polyethylene glycol 4000, 8% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.20 Å R-free 0.240
5XKH Crystal structure of T2R-TTL-CF1 complex Deposited 2017-05-07 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 89C 4-[(4-methoxy-3-oxidanyl-phenyl)-methyl-amino]chromen-2-one × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% polyethylene glycol 4000, 8% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.25 Å R-free 0.240
5XP3 Crystal structure of apo T2R-TTL Deposited 2017-05-31 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% polyethylene glycol 4000, 8% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.30 Å R-free 0.240
5YL2 Crystal structure of T2R-TTL-Y28 complex Deposited 2017-10-16 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 8WU (E)-1-(5-methoxy-2,2-dimethyl-chromen-8-yl)-3-(4-methoxy-3-oxidanyl-phenyl)prop-2-en-1-one × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% polyethylene glycol 4000, 8% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.09 Å R-free 0.254
5YLJ Crystal structure of T2R-TTL-Millepachine complex Deposited 2017-10-17 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 8X0 (E)-1-(5-methoxy-2,2-dimethyl-chromen-8-yl)-3-(4-methoxyphenyl)prop-2-en-1-one × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% polyethylene glycol 4000, 8% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.70 Å R-free 0.255
5YLS Crystal structure of T2R-TTL-Y50 complex Deposited 2017-10-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 Y50 E-3-(3-azanyl-4-methoxy-phenyl)-1-(5-methoxy-2,2-dimethyl-chromen-8-yl)prop-2-en-1-one × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% polyethylene glycol 4000, 8% glycerol, 0.1M MES, 30mM CaCl2, 30mM MgCl2, pH 6.7
Resolution 3.00 Å R-free 0.253
5Z4U Crystal Structure of T2R-TTL complex with 7a3 Deposited 2018-01-14 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 96C 4-(4-ethoxyphenyl)-1-methyl-3-(3,4,5-trimethoxyphenyl)-1H-pyrazole × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M Mes, 30 mM CaCl2, 30 mM MgCl2 (pH 6.7)
Resolution 3.18 Å R-free 0.235
5ZXH The structure of MT189-tubulin complex Deposited 2018-05-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 9LX 2-(6-fluoro-3-{[(4-methoxyphenyl)methyl]amino}imidazo[1,2-a]pyridin-2-yl)phenol × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；6% POLY(ETHYLENE GLYCOL) 4000, 8% GLYCEROL, 0.1 M MES, 30 MM CACL2, 30 MM MGCL2 (pH 6.7)
Resolution 2.80 Å R-free 0.259
6AGK The structure of CH-II-77-tubulin complex Deposited 2018-08-13 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 3 CA CALCIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 9WR [6-(3-hydroxy-4-methylphenyl)pyridin-2-yl](3,4,5-trimethoxyphenyl)methanone × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% POLYETHYLENE GLYCOL 4000, 8% GLYCEROL, 0.1M MES, 30MM CACL2, 30MM MGCL2, PH 6.7,
Resolution 2.80 Å R-free 0.264
6B0C KLP10A-AMPPNP in complex with curved tubulin and a microtubule Deposited 2017-09-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 175 PDB declaration: 175-meric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 70 MG MAGNESIUM ION × 105 GDP GUANOSINE-5'-DIPHOSPHATE × 70 TA1 TAXOL × 35 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 35 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.51 Å
6B0C KLP10A-AMPPNP in complex with curved tubulin and a microtubule Deposited 2017-09-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.51 Å
6B0C KLP10A-AMPPNP in complex with curved tubulin and a microtubule Deposited 2017-09-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.51 Å
6B0I Apo KLP10A in complex with a microtubule Deposited 2017-09-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 105 PDB declaration: 105-meric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 35 MG MAGNESIUM ION × 35 GDP GUANOSINE-5'-DIPHOSPHATE × 35 TA1 TAXOL × 35 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；with 20.10^(-3) unit of potato apyrase (Sigma-Aldrich, MO)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.78 Å
6B0I Apo KLP10A in complex with a microtubule Deposited 2017-09-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；with 20.10^(-3) unit of potato apyrase (Sigma-Aldrich, MO)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.78 Å
6B0I Apo KLP10A in complex with a microtubule Deposited 2017-09-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；with 20.10^(-3) unit of potato apyrase (Sigma-Aldrich, MO)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.78 Å
6B0L KLP10A-AMPPNP in complex with a microtubule Deposited 2017-09-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 105 PDB declaration: 105-meric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 35 MG MAGNESIUM ION × 70 GDP GUANOSINE-5'-DIPHOSPHATE × 35 TA1 TAXOL × 35 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 35 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.98 Å
6B0L KLP10A-AMPPNP in complex with a microtubule Deposited 2017-09-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.98 Å
6B0L KLP10A-AMPPNP in complex with a microtubule Deposited 2017-09-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.98 Å
6BJC TPX2_mini decorated GMPCPP-microtubule Deposited 2017-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain E 1–451（451 aa）
Chain J 1–451（451 aa）
Chain K 1–451（451 aa）
Chain L 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 12 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80
cryo-EM vitrification conditions Cryogen ETHANE；blot for 4 seconds before plunging
Resolution 3.30 Å
6BR1 Tubulin-RB3_SLD-TTL in complex with heterocyclic pyrimidine compound 4a Deposited 2017-11-29 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 E3Y 2-chloro-4-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)pyrido[2,3-d]pyrimidine × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.30 Å R-free 0.235
6BRF Tubulin-RB3_SLD-TTL in complex with heterocyclic pyrimidine compound 4b Deposited 2017-11-30 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 E44 2-chloro-4-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)pyrido[3,2-d]pyrimidine × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.50 Å R-free 0.237
6BRY Tubulin-RB3_SLD-TTL in complex with heterocyclic pyrimidine compound 6a Deposited 2017-12-01 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 GK9 1-(2-chlorofuro[3,2-d]pyrimidin-4-yl)-6-methoxy-1,2,3,4-tetrahydroquinoline × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.70 Å R-free 0.236
6BS2 Tubulin-RB3_SLD-TTL in complex with heterocyclic pyrimidine compound 8b Deposited 2017-12-01 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 E9Y 1-(3,6-dimethyl[1,2]oxazolo[5,4-d]pyrimidin-4-yl)-6-methoxy-1,2,3,4-tetrahydroquinoline × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.65 Å R-free 0.234
6CVJ Model of synthetic tau (four tandem repeats of first repeat sequence) bound to the microtubule Deposited 2018-03-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 120 PDB declaration: 120-meric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 30 MG MAGNESIUM ION × 30 GDP GUANOSINE-5'-DIPHOSPHATE × 60 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash
Resolution 3.20 Å
6CVJ Model of synthetic tau (four tandem repeats of first repeat sequence) bound to the microtubule Deposited 2018-03-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash
Resolution 3.20 Å
6CVJ Model of synthetic tau (four tandem repeats of first repeat sequence) bound to the microtubule Deposited 2018-03-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash
Resolution 3.20 Å
6CVN Model of synthetic tau (R2x4) bound to the microtubule Deposited 2018-03-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 120 PDB declaration: 120-meric
Chain B 1–451（451 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 60 GTP GUANOSINE-5'-TRIPHOSPHATE × 30 MG MAGNESIUM ION × 30 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash.
Resolution 3.90 Å
6CVN Model of synthetic tau (R2x4) bound to the microtubule Deposited 2018-03-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–451（451 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash.
Resolution 3.90 Å
6CVN Model of synthetic tau (R2x4) bound to the microtubule Deposited 2018-03-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–451（451 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash.
Resolution 3.90 Å
6D88 Tubulin-RB3_SLD-TTL in complex with compound 13f Deposited 2018-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 G9K [2-(1H-indol-3-yl)-1H-imidazol-4-yl](8-methoxy-1,4-benzodioxin-6-yl)methanone × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 2.85 Å R-free 0.246
6DPU Undecorated GMPCPP microtubule Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain E 1–451（451 aa）
Chain J 1–451（451 aa）
Chain K 1–451（451 aa）
Chain L 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 12 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 buffer (80 mM PIPES, pH 6.8, 1 mM EGTA, 1 mM MgCl2) supplemented with 1 mM GMPCPP, 1 mM DTT, and 0.05% Nonident P-40
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.
Resolution 3.10 Å
6DPV Undecorated GDP microtubule Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain E 1–451（451 aa）
Chain J 1–451（451 aa）
Chain K 1–451（451 aa）
Chain L 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 buffer (80 mM PIPES pH 6.8, 1 mM EGTA, 1 mM MgCl2) supplemented with 1 mM GTP, 1 mM DTT, and 0.05% Nonident P-40
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.
Resolution 3.30 Å
6DPW Undecorated GTPgammaS microtubule Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain E 1–451（451 aa）
Chain J 1–451（451 aa）
Chain K 1–451（451 aa）
Chain L 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；Modified BRB80 buffer (80 mM PIPES, pH 6.8, 1 mM EGTA, 0.2 mM MgCl2) supplemented with 1 mM GTPgammaS, 1 mM DTT, and 0.05% Nonident P-40
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.
Resolution 3.50 Å
6EG5 The structure of SB-1-202-tubulin complex Deposited 2018-08-18 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 6 CA CALCIUM ION × 4 CL CHLORIDE ION × 1 GOL GLYCEROL × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 J7S 4-(2-chloropyrido[2,3-d]pyrimidin-4-yl)-7-methoxy-3,4-dihydroquinoxalin-2(1H)-one × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% POLYETHYLENE GLYCOL 4000, 8% GLYCEROL, 0.1 M MES, 30 MM CACL2, 30 MM MGCL2, PH 6.7
Resolution 2.45 Å R-free 0.236
6EVW Cryo-EM structure of GMPCPP-microtubule co-polymerised with doublecortin Deposited 2017-11-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 1–438（438 aa）
Chain C 1–438（438 aa）
Chain E 1–438（438 aa）
Chain J 1–438（438 aa）
Chain K 1–438（438 aa）
Chain L 1–438（438 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 12 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
6EVX Cryo-EM structure of GDP.Pi-microtubule rapidly co-polymerised with doublecortin Deposited 2017-11-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain E 1–451（451 aa）
Chain J 1–451（451 aa）
Chain K 1–451（451 aa）
Chain L 1–451（451 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 PO4 PHOSPHATE ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
6EVY Cryo-EM structure of GTPgammaS-microtubule co-polymerised with doublecortin Deposited 2017-11-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain E 1–451（451 aa）
Chain J 1–451（451 aa）
Chain K 1–451（451 aa）
Chain L 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
6EVZ Cryo-EM structure of GDP-microtubule co-polymerised with doublecortin Deposited 2017-11-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain E 1–451（451 aa）
Chain J 1–451（451 aa）
Chain K 1–451（451 aa）
Chain L 1–451（451 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6EW0 Cryo-EM structure of GDP-microtubule co-polymerised with doublecortin and supplemented with Taxol Deposited 2017-11-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain E 1–451（451 aa）
Chain J 1–451（451 aa）
Chain K 1–451（451 aa）
Chain L 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 TA1 TAXOL × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6JCJ Structure of crolibulin in complex with tubulin Deposited 2019-01-29 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 3 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 BG0 (4R)-2,7,8-triamino-4-(3-bromo-4,5-dimethoxyphenyl)-4H-1-benzopyran-3-carbonitrile × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% POLYETHYLENE GLYCOL 4000, 8% GLYCEROL, 0.1 M MES, 30 MM CACL2, 30 MM MGCL2, PH 6.7
Resolution 2.50 Å R-free 0.282
6KPP BNC105 in complex with tubulin Deposited 2019-08-15 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 DO6 (6-methoxy-2-methyl-7-oxidanyl-1-benzofuran-3-yl)-(3,4,5-trimethoxyphenyl)methanone × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；277 K；6% PEG, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.75 Å R-free 0.235
6LS4 A novel anti-tumor agent S-40 in complex with tubulin Deposited 2020-01-17 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 GOL GLYCEROL × 1 S40 3-[(4-cyclopropylphenyl)sulfonylamino]-4-methyl-N-(pyridin-3-ylmethyl)benzamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；6% PEG 4000, 6% GLYCEROL, 30mM MAGNESIUM CHLORIDE, 30mM CALCIUM CHLORIDE, 3.3% Jeffamin M-600, 100mM MES
Resolution 2.40 Å R-free 0.240
6LSM Tubulin Polymerization Inhibitors Deposited 2020-01-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 6 CA CALCIUM ION × 4 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 ERX 2-(4-methylphenyl)-7-(3,4,5-trimethoxyphenyl)pyrazolo[1,5-a]pyrimidine × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；277 K；6% PEG, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.75 Å R-free 0.227
6LSN Crystal structure of tubulin-inhibitor complex Deposited 2020-01-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 6 CA CALCIUM ION × 4 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 ERR 2-(1-methylindol-5-yl)-7-(3,4,5-trimethoxyphenyl)pyrazolo[1,5-a]pyrimidine × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；277 K；6% PEG, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.44 Å R-free 0.220
6N47 The structure of SB-2-204-tubulin complex Deposited 2018-11-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 CA CALCIUM ION × 4 CL CHLORIDE ION × 1 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 KB4 4-(2-chloropyrido[3,2-d]pyrimidin-4-yl)-7-methoxy-3,4-dihydroquinoxalin-2(1H)-one × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% POLYETHYLENE GLYCOL 4000, 8% GLYCEROL, 0.1 M MES, 30 MM CACL2, 30 MM MGCL2, PH 6.7
Resolution 2.60 Å R-free 0.246
6NNG Tubulin-RB3_SLD-TTL in complex with compound DJ95 Deposited 2019-01-15 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 DJ9 2-(1H-indol-6-yl)-4-(3,4,5-trimethoxyphenyl)-1H-imidazo[4,5-c]pyridine × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 2.40 Å R-free 0.227
6O2Q Acetylated Microtubules Deposited 2019-02-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain E 1–451（451 aa）
Chain J 1–451（451 aa）
Chain K 1–451（451 aa）
Chain L 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；Contains 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH (stored at 4 degrees Celsius).
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 4 seconds at blot force 10.
Resolution 3.70 Å
6O2R Deacetylated Microtubules Deposited 2019-02-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain E 1–451（451 aa）
Chain J 1–451（451 aa）
Chain K 1–451（451 aa）
Chain L 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；Contains 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH (stored at 4 degrees Celsius).
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 4 seconds at blot force 10.
Resolution 3.30 Å
6O2S Deacetylated Microtubules Deposited 2019-02-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 104 PDB declaration: 104-meric
Chain 1A 1–451（451 aa）
Chain 1B 1–451（451 aa）
Chain 1C 1–451（451 aa）
Chain 1D 1–451（451 aa）
Chain 1E 1–451（451 aa）
Chain 1F 1–451（451 aa）
Chain 1G 1–451（451 aa）
Chain 1I 1–451（451 aa）
Chain 1J 1–451（451 aa）
Chain 1K 1–451（451 aa）
Chain 1L 1–451（451 aa）
Chain 1M 1–451（451 aa）
Chain 1N 1–451（451 aa）
Chain 2A 1–451（451 aa）
Chain 2B 1–451（451 aa）
Chain 2C 1–451（451 aa）
Chain 2D 1–451（451 aa）
Chain 2E 1–451（451 aa）
Chain 2F 1–451（451 aa）
Chain 2G 1–451（451 aa）
Chain 2I 1–451（451 aa）
Chain 2J 1–451（451 aa）
Chain 2K 1–451（451 aa）
Chain 2L 1–451（451 aa）
Chain 2M 1–451（451 aa）
Chain 2N 1–451（451 aa）
Chain 3A 1–451（451 aa）
Chain 3B 1–451（451 aa）
Chain 3C 1–451（451 aa）
Chain 3D 1–451（451 aa）
Chain 3E 1–451（451 aa）
Chain 3F 1–451（451 aa）
Chain 3G 1–451（451 aa）
Chain 3I 1–451（451 aa）
Chain 3J 1–451（451 aa）
Chain 3K 1–451（451 aa）
Chain 3L 1–451（451 aa）
Chain 3M 1–451（451 aa）
Chain 3N 1–451（451 aa）
Chain 4A 1–451（451 aa）
Chain 4B 1–451（451 aa）
Chain 4C 1–451（451 aa）
Chain 4D 1–451（451 aa）
Chain 4E 1–451（451 aa）
Chain 4F 1–451（451 aa）
Chain 4G 1–451（451 aa）
Chain 4I 1–451（451 aa）
Chain 4J 1–451（451 aa）
Chain 4K 1–451（451 aa）
Chain 4L 1–451（451 aa）
Chain 4M 1–451（451 aa）
Chain 4N 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 52 MG MAGNESIUM ION × 52 GDP GUANOSINE-5'-DIPHOSPHATE × 52 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；Contains 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH (stored at 4 degrees Celsius).
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 4 seconds at blot force 10.
Resolution 4.00 Å
6O2T Acetylated Microtubules Deposited 2019-02-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 104 PDB declaration: 104-meric
Chain 1A 1–451（451 aa）
Chain 1B 1–451（451 aa）
Chain 1C 1–451（451 aa）
Chain 1D 1–451（451 aa）
Chain 1E 1–451（451 aa）
Chain 1F 1–451（451 aa）
Chain 1G 1–451（451 aa）
Chain 1I 1–451（451 aa）
Chain 1J 1–451（451 aa）
Chain 1K 1–451（451 aa）
Chain 1L 1–451（451 aa）
Chain 1M 1–451（451 aa）
Chain 1N 1–451（451 aa）
Chain 2A 1–451（451 aa）
Chain 2B 1–451（451 aa）
Chain 2C 1–451（451 aa）
Chain 2D 1–451（451 aa）
Chain 2E 1–451（451 aa）
Chain 2F 1–451（451 aa）
Chain 2G 1–451（451 aa）
Chain 2I 1–451（451 aa）
Chain 2J 1–451（451 aa）
Chain 2K 1–451（451 aa）
Chain 2L 1–451（451 aa）
Chain 2M 1–451（451 aa）
Chain 2N 1–451（451 aa）
Chain 3A 1–451（451 aa）
Chain 3B 1–451（451 aa）
Chain 3C 1–451（451 aa）
Chain 3D 1–451（451 aa）
Chain 3E 1–451（451 aa）
Chain 3F 1–451（451 aa）
Chain 3G 1–451（451 aa）
Chain 3I 1–451（451 aa）
Chain 3J 1–451（451 aa）
Chain 3K 1–451（451 aa）
Chain 3L 1–451（451 aa）
Chain 3M 1–451（451 aa）
Chain 3N 1–451（451 aa）
Chain 4A 1–451（451 aa）
Chain 4B 1–451（451 aa）
Chain 4C 1–451（451 aa）
Chain 4D 1–451（451 aa）
Chain 4E 1–451（451 aa）
Chain 4F 1–451（451 aa）
Chain 4G 1–451（451 aa）
Chain 4I 1–451（451 aa）
Chain 4J 1–451（451 aa）
Chain 4K 1–451（451 aa）
Chain 4L 1–451（451 aa）
Chain 4M 1–451（451 aa）
Chain 4N 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 52 MG MAGNESIUM ION × 52 GDP GUANOSINE-5'-DIPHOSPHATE × 52 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；Contains 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH (stored at 4 degrees Celsius).
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 4 seconds at blot force 10.
Resolution 4.10 Å
6O5M Tubulin-RB3_SLD-TTL in complex with compound 10bb Deposited 2019-03-04 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 CA CALCIUM ION × 2 MG MAGNESIUM ION × 4 GOL GLYCEROL × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 G8K [2-(1H-indol-4-yl)-1H-imidazol-4-yl](3,4,5-trimethoxyphenyl)methanone × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 2.30 Å R-free 0.223
6O5N Tubulin-RB3_SLD-TTL in complex with compound 10ab Deposited 2019-03-04 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 QW9 [2-(4-methyl-1H-indol-3-yl)-1H-imidazol-5-yl](3,4,5-trimethoxyphenyl)methanone × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 3.00 Å R-free 0.245
6O61 Tubulin-RB3_SLD-TTL in complex with compound ABI-231 Deposited 2019-03-05 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 KUM [2-(1H-indol-3-yl)-1H-imidazol-5-yl](3,4,5-trimethoxyphenyl)methanone × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 2.60 Å R-free 0.243
6PC4 Tubulin-RB3_SLD-TTL in complex with compound ABI-274 Deposited 2019-06-15 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 O91 [2-(4-methylphenyl)-1H-imidazol-4-yl](3,4,5-trimethoxyphenyl)methanone × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 2.60 Å R-free 0.234
6RZA Cryo-EM structure of the human inner arm dynein DNAH7 microtubule binding domain bound to microtubules Deposited 2019-06-13 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–437（437 aa）
Chain C 1–437（437 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
6RZB Cryo-EM structure of mouse cytoplasmic dynein-1 microtubule binding domain bound to microtubules Deposited 2019-06-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–437（437 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
6TA3 Human kinesin-5 motor domain in the GSK-1 state bound to microtubules (Conformation 1) Deposited 2019-10-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–438（438 aa）
Not recorded G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 MG MAGNESIUM ION × 2 MZK 6-[4-(trifluoromethyl)phenyl]-3,4-dihydro-1~{H}-quinolin-2-one × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6TA4 Human kinesin-5 motor domain in the AMPPNP state bound to microtubules Deposited 2019-10-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–438（438 aa）
Not recorded G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.10 Å
6TIW Human kinesin-5 motor domain in the GSK state bound to microtubules (Conformation 2) Deposited 2019-11-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–438（438 aa）
Not recorded MZK 6-[4-(trifluoromethyl)phenyl]-3,4-dihydro-1~{H}-quinolin-2-one × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6WWE Apo KIF14[391-772] in complex with a microtubule Deposited 2020-05-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6WWF KIF14[391-772] - ADP in complex with a microtubule Deposited 2020-05-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6WWG KIF14[391-772] dimer two-heads-bound state - ADP-AlFx in complex with a microtubule Deposited 2020-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain E 1–451（451 aa）
Not recorded AF3 ALUMINUM FLUORIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
6WWH KIF14[391-772] dimer two-heads-bound state - AMP-PNP in complex with a microtubule Deposited 2020-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain E 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6WWI Apo KIF14[391-755] in complex with a microtubule Deposited 2020-05-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6WWJ KIF14[391-755] - ADP in complex with a microtubule Deposited 2020-05-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6WWK KIF14[391-755] dimer two-heads-bound state - ADP-AlFx in complex with a microtubule Deposited 2020-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain E 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 AF3 ALUMINUM FLUORIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6WWL KIF14[391-755] dimer two-heads-bound state - AMP-PNP in complex with a microtubule Deposited 2020-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain E 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6WWM KIF14[391-748] - ADP in complex with a microtubule Deposited 2020-05-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6WWN KIF14[391-748] - ADP-AlFx in complex with a microtubule Deposited 2020-05-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 AF3 ALUMINUM FLUORIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
6WWO KIF14[391-748] - AMP-PNP in complex with a microtubule Deposited 2020-05-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6WWP Apo KIF14[391-743] in complex with a microtubule Deposited 2020-05-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6WWQ KIF14[391-743] - ADP in complex with a microtubule Deposited 2020-05-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6WWR Kif14[391-743] - ADP-AlFx open state class in complex with a microtubule Deposited 2020-05-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
6WWS Kif14[391-743] - AMP-PNP open state class in complex with a microtubule Deposited 2020-05-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
6WWT Apo KIF14[391-735] in complex with a microtubule Deposited 2020-05-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6WWU KIF14[391-735] - ADP-AlFx in complex with a microtubule Deposited 2020-05-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
6WWV KIF14[391-735] - ANP-PNP in complex with a microtubule Deposited 2020-05-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6X1C Tubulin-RB3_SLD-TTL in complex with compound 5j Deposited 2020-05-18 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 CA CALCIUM ION × 2 MG MAGNESIUM ION × 5 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 Y5J 4-(2-chlorofuro[3,2-d]pyrimidin-4-yl)-7-methoxy-3,4-dihydroquinoxalin-2(1H)-one × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% PEG 4000, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.90 Å R-free 0.216
6X1E Tubulin-RB3_SLD-TTL in complex with compound 5l Deposited 2020-05-18 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 CA CALCIUM ION × 2 MG MAGNESIUM ION × 5 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 Y5L 4-(2-chloro-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl)-7-methoxy-3,4-dihydroquinoxalin-2(1H)-one × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% PEG 4000, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.90 Å R-free 0.224
6X1F Tubulin-RB3_SLD-TTL in complex with compound 5m Deposited 2020-05-18 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 CA CALCIUM ION × 2 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 Y5M 7-methoxy-4-(2-methyl-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl)-3,4-dihydroquinoxalin-2(1H)-one × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；6% PEG 4000, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.70 Å R-free 0.241
6XER Tubulin-RB3_SLD in complex with colchicine Deposited 2020-06-13 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–438（438 aa）
Chain C 1–438（438 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 2 SO4 SULFATE ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 LOC N-[(7S)-1,2,3,10-tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[d]heptalen-7-yl]ethanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 5.6；293 K；PEG 4000, ammonium sulfate, tri-sodium citrate
Resolution 2.50 Å R-free 0.223
6XES Tubulin-RB3_SLD in complex with compound 40a Deposited 2020-06-13 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–438（438 aa）
Chain C 1–438（438 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 SO4 SULFATE ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TU3 [6-(3-hydroxy-4-methylphenyl)pyrazin-2-yl](3,4,5-trimethoxyphenyl)methanone × 2 MG MAGNESIUM ION × 1 DMS DIMETHYL SULFOXIDE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.6；293 K；PEG 4000, ammonium sulfate, tri-sodium citrate
Resolution 2.32 Å R-free 0.226
6XET Tubulin-RB3_SLD in complex with compound 60c Deposited 2020-06-13 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–438（438 aa）
Chain C 1–438（438 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 SO4 SULFATE ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TU2 [3-fluoro-6-(3-hydroxy-4-methylphenyl)pyridin-2-yl](3,4,5-trimethoxyphenyl)methanone × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.6；293 K；PEG 4000, ammonium sulfate, tri-sodium citrate
Resolution 2.60 Å R-free 0.231
6Y4M Structure of Tubulin Tyrosine Ligase in Complex with Tb111 Deposited 2020-02-21 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 O9B (2~{R})-1-methylpiperidine-2-carboxylic acid × 1 O9H [(1~{R},3~{R})-1-acetyloxy-1-[4-methanoyl-5-(2-phenylmethoxyethyl)-2,3-dihydro-1,3-thiazol-2-yl]-4-methyl-pentan-3-yl]-methyl-azanium × 1 OH5 (2~{S},4~{R})-4-azanyl-2-methyl-5-phenyl-pentanoic acid × 1 PGE TRIETHYLENE GLYCOL × 1 VAL VALINE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.03 M calcium chloride, 0.03 M magnesium chloride, 0.10 M MES pH 6.50, 3.20% w/v PEG 4000, 5.00% w/v glycerol
Resolution 3.34 Å R-free 0.234
6Y4N Structure of Tubulin Tyrosine Ligase in Complex with Tb116 Deposited 2020-02-21 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 O9B (2~{R})-1-methylpiperidine-2-carboxylic acid × 1 O9K [(1~{R},3~{R})-1-(4-methanoyl-1,3-thiazol-2-yl)-4-methyl-3-(methylamino)pentyl] ethanoate × 1 O9N methyl (2~{S},4~{S})-2,4-bis(azanyl)-5-phenyl-pentanoate × 1 PGE TRIETHYLENE GLYCOL × 1 VAL VALINE × 1 P6S benzyl hydrogen carbonate × 1 PEG DI(HYDROXYETHYL)ETHER × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；30 mM calcium chloride, 30 mM magnesium chloride, 0.10 M MES pH 6.0, 2.0% w/v PEG 4000, 5.0% w/v glycerol
Resolution 2.85 Å R-free 0.220
6ZPI Microtubule complexed with Kif15 motor domain. Symmetrised asymmetric unit Deposited 2020-07-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–437（437 aa）
Not recorded ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
7CBZ Crystal structure of T2R-TTL-A31 complex Deposited 2020-06-15 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 FUO 2-[5-[4-[2-[4-(2-cyclopropylethanoyl)piperazin-1-yl]ethoxy]phenyl]pyridin-2-yl]-N-(phenylmethyl)ethanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, and 30 mM MgCl2.
Resolution 2.61 Å R-free 0.255
7CDA Crystal structure of T2R-TTL-PAC complex Deposited 2020-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 AEU N-[(3-phenoxyphenyl)methyl]-9H-beta-carbolin-3-amine × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2 and 30 mM MgCl2.
Resolution 2.66 Å R-free 0.257
7CE6 Crystal structure of T2R-TTL-Compound9 complex Deposited 2020-06-22 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 AF6 N-benzyl-9H-beta-carbolin-3-amine × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2 and 30 mM MgCl2.
Resolution 2.69 Å R-free 0.248
7CE8 Crystal structure of T2R-TTL-Compound11 complex Deposited 2020-06-22 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 AEX N-butyl-9H-beta-carbolin-3-amine × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2 and 30 mM MgCl2
Resolution 2.73 Å R-free 0.242
7CEK Crystal structure of T2R-TTL-BML-284 complex Deposited 2020-06-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 FW9 N4-(1,3-benzodioxol-5-ylmethyl)-6-(3-methoxyphenyl)pyrimidine-2,4-diamine × 2 CL CHLORIDE ION × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2 and 30 mM MgCl2.
Resolution 2.70 Å R-free 0.271
7CLD Crystal structure of T2R-TTL-Cevipabulin complex Deposited 2020-07-20 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 G2X 6-[2,6-bis(fluoranyl)-4-[3-(methylamino)propoxy]phenyl]-5-chloranyl-N-[(2S)-1,1,1-tris(fluoranyl)propan-2-yl]-[1,2,4]triazolo[1,5-a]pyrimidin-7-amine × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2 and 30 mM MgCl2.
Resolution 2.61 Å R-free 0.258
7CNM YDX in complex with tubulin Deposited 2020-08-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 G70 (2~{S},4~{S})-4-[[2-[(1~{R},3~{R})-1-acetyloxy-4-methyl-3-[[(2~{S},3~{S})-3-methyl-2-[[(2~{R})-1-methylpiperidin-2-yl]carbonylamino]pentanoyl]amino]pentyl]-1,3-thiazol-4-yl]carbonylamino]-5-cyclohexyl-2-methyl-pentanoic acid × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；277 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.44 Å R-free 0.237
7CNN vinorelbine in complex with tubulin Deposited 2020-08-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 4 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 GDF Vinorelbine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；277 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.50 Å R-free 0.229
7CNO Phomopsin A in complex with tubulin Deposited 2020-08-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 4 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 HOS Phomopsin A × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；277 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCL2, 30 mM MgCl2, pH 6.7
Resolution 2.50 Å R-free 0.239
7DAD EPD in complex with tubulin Deposited 2020-10-16 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 4 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 EPD EPOTHILONE D × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；277 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCL2, pH 6.7
Resolution 2.85 Å R-free 0.226
7DAE EPB in complex with tubulin Deposited 2020-10-16 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 4 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 EPB 7,11-DIHYDROXY-8,8,10,12,16-PENTAMETHYL-3-[1-METHYL-2-(2-METHYL-THIAZOL-4-YL)VINYL]-4,17-DIOXABICYCLO[14.1.0]HEPTADECANE-5,9-DIONE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；277 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCL2, pH 6.7
Resolution 2.39 Å R-free 0.222
7DAF IXA in complex with tubulin Deposited 2020-10-16 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 6 CA CALCIUM ION × 4 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 GZX (1~{S},3~{S},7~{S},10~{R},11~{S},12~{S},16~{R})-8,8,10,12,16-pentamethyl-3-[(~{E})-1-(2-methyl-1,3-thiazol-4-yl)prop-1-en-2-yl]-7,11-bis(oxidanyl)-17-oxa-4-azabicyclo[14.1.0]heptadecane-5,9-dione × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；277 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCL2, pH 6.7
Resolution 2.40 Å R-free 0.222
7DB9 IC1 in complex with tubulin Deposited 2020-10-19 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 5 CA CALCIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 IC1 3-[(2,4,6-TRIMETHOXY-PHENYL)-METHYLENE]-INDOLIN-2-ONE × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；277 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCL2, pH 6.7
Resolution 2.85 Å R-free 0.233
7DBA RYX in complex with tubulin Deposited 2020-10-19 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 H1O 2-(1-methylindol-4-yl)-7-(3,4,5-trimethoxyphenyl)-1~{H}-benzimidazole × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；277 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCL2, pH 6.7
Resolution 2.46 Å R-free 0.228
7DBB SSE in complex with tubulin Deposited 2020-10-19 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 H1F 5-phenyl-3-(3,4,5-trimethoxyphenyl)-3,4-dihydropyrazole-2-carbothioamide × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；277 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCL2, pH 6.7
Resolution 2.81 Å R-free 0.230
7DBC PRA in complex with tubulin Deposited 2020-10-19 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 2 H1C methyl ~{N}-(5-butyl-1~{H}-benzimidazol-2-yl)carbamate × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；277 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCL2, pH 6.7
Resolution 2.40 Å R-free 0.234
7DBD 444 in complex with tubulin Deposited 2020-10-19 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 H0U ~{N}-[5-(5-cyanothiophen-2-yl)-2-methyl-phenyl]-4-methyl-benzenesulfonamide × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；277 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCL2, pH 6.7
Resolution 3.09 Å R-free 0.244
7DMZ GMPCPP microtubule complex Deposited 2020-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain B 1–451（451 aa）
Chain D 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 2 TAJ taccalonolide AJ × 3 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
7DN0 AJ-GMPCPP-MT-non-seam Deposited 2020-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain B 1–451（451 aa）
Chain C 1–451（451 aa）
Chain D 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 4 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 TAJ taccalonolide AJ × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
7DP8 Crystal structure of T2R-TTL-Cevipabulin-eribulin complex Deposited 2020-12-18 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 GOL GLYCEROL × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 G2X 6-[2,6-bis(fluoranyl)-4-[3-(methylamino)propoxy]phenyl]-5-chloranyl-N-[(2S)-1,1,1-tris(fluoranyl)propan-2-yl]-[1,2,4]triazolo[1,5-a]pyrimidin-7-amine × 2 6K9 (1S,3S,6S,9S,12S,14R,16R,18S,20R,21R,22S,26R,29S,31R,32S,33R,35R,36S)-20-[(2S)-3-amino-2-hydroxypropyl]-21-methoxy-14-methyl-8,15-dimethylidene-2,19,30,34,37,39,40,41-octaoxanonacyclo[24.9.2.1~3,32~.1~3,33~.1~6,9~.1~12,16~.0~18,22~.0~29,36~.0~31,35~]hentetracontan-24-one (non-preferred name) × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2 and 30 mM MgCl2
Resolution 2.45 Å R-free 0.264
7EMJ Crystal structure of T2R-TTL-Barbigerone complex Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 J6L 8,8-dimethyl-3-(2,4,5-trimethoxyphenyl)pyrano[2,3-f]chromen-4-one × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1 M MES, 30 mM CaCl2 and 30 mM MgCl2, pH 6.7
Resolution 2.33 Å R-free 0.241
7EN3 Crystal structure of tubulin in complex with Tubulysin analogue TGL Deposited 2021-04-15 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 J6R (2S,4R)-5-(4-fluorophenyl)-2-methyl-4-[[2-[(1R,3R)-4-methyl-3-[5-methylhexyl-[(2S,3S)-3-methyl-2-[[(2R)-1-methylpiperidin-2-yl]carbonylamino]pentanoyl]amino]-1-oxidanyl-pentyl]-1,3-thiazol-4-yl]carbonylamino]pentanoic acid × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 2.64 Å R-free 0.239
7EXC Crystal structure of T2R-TTL-1129A2 complex Deposited 2021-05-26 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 JEL N-[[3-(1,3-benzodioxol-5-yloxy)phenyl]methyl]-9H-pyrido[3,4-b]indol-3-amine × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, 30 mM MgCl2
Resolution 2.39 Å R-free 0.264
7L05 Complex of novel maytansinoid M24 bound to T2R-TTL (two tubulin alpha/beta heterodimers, RB3 stathmin-like domain, and tubulin tyrosine ligase) Deposited 2020-12-11 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 5 CA CALCIUM ION × 4 CL CHLORIDE ION × 1 EDO 1,2-ETHANEDIOL × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 XQ4 (1S,2R,3S,5S,6S,16E,18E,20R,21S)-11-chloro-21-hydroxy-12,20-dimethoxy-2,5,9,16-tetramethyl-8,23-dioxo-4,24-dioxa-9,22-diazatetracyclo[19.3.1.1~10,14~.0~3,5~]hexacosa-10(26),11,13,16,18-pentaen-6-yl (2S)-2-{methyl[3-(methylamino)propanoyl]amino}propanoate (non-preferred name) × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；PEG 3350, MES, CaCl2, MgCl2, glycerol
Resolution 2.21 Å R-free 0.217
7LVQ KIF14[391-743] - AMP-PNP closed state class in complex with a microtubule Deposited 2021-02-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7LVR KIF14[391-743] - ADP-AlFx closed state class in complex with a microtubule Deposited 2021-02-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 AF3 ALUMINUM FLUORIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7LZ7 Tubulin-RB3_SLD-TTL in complex with compound 5k Deposited 2021-03-09 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 CA CALCIUM ION × 2 MG MAGNESIUM ION × 5 YJ7 4-(3,6-dimethyl[1,2]oxazolo[5,4-d]pyrimidin-4-yl)-7-methoxy-3,4-dihydroquinoxalin-2(1H)-one × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；PEG 4000, glycerol, MES, CaCl2, MgCl2
Resolution 2.80 Å R-free 0.211
7LZ8 Tubulin-RB3_SLD-TTL in complex with compound 5t Deposited 2021-03-09 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 CA CALCIUM ION × 4 MG MAGNESIUM ION × 4 CL CHLORIDE ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 YJ4 4-[2-(ethylamino)pyrido[3,2-d]pyrimidin-4-yl]-7-methoxy-3,4-dihydroquinoxalin-2(1H)-one × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；PEG 4000, MES, glycerol, CaCl2, MgCl2, ethyl acetate
Resolution 2.92 Å R-free 0.228
7NB8 Plasmodium falciparum kinesin-5 motor domain without nucleotide, complexed with 14 protofilament microtubule. Deposited 2021-01-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 uM microtubules were incubated to the grid and incubated for 30 seconds. The grid was blotted, then 40 uM kinesin motor domain added and incubated for 30 seconds. The grid was again blotted and 40 uM kinesin motor domain added for 30 seconds. Then the grid was blotted and plunge frozen.
Resolution 4.40 Å
7NBA Plasmodium falciparum kinesin-5 motor domain bound to AMPPNP, complexed with 14 protofilament microtubule. Deposited 2021-01-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；5 uM microtubules were applied to the grid and incubated for 30 seconds. The grid was blotted, then 40 uM kinesin motor domain added, incubated for 30 seconds, then blotted. 40 uM Kinesin motor domain was again added, incubated for 40 seconds, blotted, then plunge frozen.
Resolution 4.00 Å
7PQC tau-microtubule structural ensemble based on CryoEM data Deposited 2021-09-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain B 1–451（451 aa）
Chain D 1–451（451 aa）
Chain F 1–451（451 aa）
Chain H 1–451（451 aa）
Chain J 1–451（451 aa）
Chain L 1–451（451 aa）
Chain N 1–451（451 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 7 MG MAGNESIUM ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7PQP tau-microtubule structural ensemble based on CryoEM data Deposited 2021-09-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain B 1–451（451 aa）
Chain D 1–451（451 aa）
Chain F 1–451（451 aa）
Chain H 1–451（451 aa）
Chain J 1–451（451 aa）
Chain L 1–451（451 aa）
Chain N 1–451（451 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 7 MG MAGNESIUM ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7RS6 Cryo-EM structure of Kip3 (AMPPNP) bound to GMPCPP-Stabilized Microtubules Deposited 2021-08-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain E 1–451（451 aa）
Chain G 1–451（451 aa）
Chain I 1–451（451 aa）
Chain L 1–451（451 aa）
Chain N 1–451（451 aa）
Chain P 1–451（451 aa）
Chain R 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 27 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 9 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 buffer (80 mM PIPES-KOH, pH 6.8; 1 mM MgCl2, 1 mM EGTA, 1 mM DTT)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7SGS Cryo-EM structure of full-length MAP7 bound to the microtubule Deposited 2021-10-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–451（451 aa）
Chain D 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7TQX CaKip3[2-482] - AMP-PNP in complex with a microtubule Deposited 2022-01-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7TQY CaKip3[2-482] - ADP-AlFx in complex with a microtubule Deposited 2022-01-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded AF3 ALUMINUM FLUORIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
7TQZ Apo CaKip3[2-482] in complex with a microtubule Deposited 2022-01-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7TR0 CaKip3[2-436] - AMP-PNP in complex with a microtubule Deposited 2022-01-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7TR1 CaKip3[2-436]-L2-mutant(HsKHC) - AMP-PNP in complex with a microtubule Deposited 2022-01-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TR2 Apo CaKip3[2-436]-L2-mutant(HsKHC) in complex with a microtubule Deposited 2022-01-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
7TR3 CaKip3[2-482] - AMP-PNP in complex with a dolastatin-10-stabilized tubulin ring Deposited 2022-01-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 SR6 dolastatin-10 × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
7TTD Tubulin-RB3_SLD in complex with compound 12e Deposited 2022-02-01 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–438（438 aa）
Chain C 1–438（438 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 SO4 SULFATE ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 JUL 7-methoxy-4-[2-(morpholin-4-yl)-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl]-3,4-dihydroquinoxalin-2(1H)-one × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 5.6；293 K；0.2M ammonium sulfate, 0.1 M tri-sodium citrate, pH 5.6, 12 ~14 % PEG 4K
Resolution 2.27 Å R-free 0.224
7TTE Tubulin-RB3_SLD in complex with compound 12j Deposited 2022-02-01 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–438（438 aa）
Chain C 1–438（438 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 SO4 SULFATE ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 JVR 4-[2-(cyclopropylamino)-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl]-7-methoxy-3,4-dihydroquinoxalin-2(1H)-one × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 5.6；293 K；0.2M ammonium sulfate, 0.1 M tri-sodium citrate, pH 5.6, 14 % PEG 4K
Resolution 2.70 Å R-free 0.239
7TTF Tubulin-RB3_SLD in complex with compound 12k Deposited 2022-02-01 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–438（438 aa）
Chain C 1–438（438 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 SO4 SULFATE ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 JV9 7-methoxy-4-[2-(methylamino)-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl]-3,4-dihydroquinoxalin-2(1H)-one × 2 GOL GLYCEROL × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 5.6；293 K；0.2M ammonium sulfate, 0.1 M tri-sodium citrate, pH 5.6, 14 % PEG 4K
Resolution 2.10 Å R-free 0.238
7XQX Crystal structure of T2R-TTL-27a complex Deposited 2022-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 CA CALCIUM ION × 2 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 GXI 2-chloranyl-7-fluoranyl-N-(4-methoxyphenyl)-N-methyl-quinazolin-4-amine × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；277 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 3.36 Å R-free 0.245
7XQY Crystal structure of T2R-TTL-15 complex Deposited 2022-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 CA CALCIUM ION × 2 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 GX5 2-chloranyl-N-(4-methoxyphenyl)-N-methyl-pyrido[3,2-d]pyrimidin-4-amine × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；277 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.35 Å R-free 0.236
7XR0 Crystal structure of T2R-TTL-27a complex Deposited 2022-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 CA CALCIUM ION × 2 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 GWC 2-chloranyl-5-fluoranyl-N-(4-methoxyphenyl)-N-methyl-quinazolin-4-amine × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；277 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.70 Å R-free 0.246
7XR1 Crystal structure of T2R-TTL-3a complex Deposited 2022-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 CA CALCIUM ION × 2 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 GY2 2-chloranyl-6-fluoranyl-N-(4-methoxyphenyl)-N-methyl-quinazolin-4-amine × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；277 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.81 Å R-free 0.246
7YHN ANTI-TUMOR AGENT Y48 IN COMPLEX WITH TUBULIN Deposited 2022-07-14 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 IUK 4-methyl-3-[(4-methylphenyl)sulfonylamino]-~{N}-[(6-methylpyridin-3-yl)methyl]benzamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；6% PEG 4000, 8% GLYCEROL, 30MM MAGNESIUM CHLORIDE, 30MM CALCIUM CHLORIDE, 100MM MES
Resolution 2.60 Å R-free 0.272
7YSN Tubulin heterodimer structure of GMPCPP state in solution Deposited 2022-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
7YSO Tubulin heterodimer structure of GDP-1 state in solution Deposited 2022-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7YSP Tubulin heterodimer structure of GDP-2 state in solution Deposited 2022-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
7Z2A P. berghei kinesin-8B motor domain in no nucleotide state bound to tubulin dimer Deposited 2022-02-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–437（437 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
7Z2B P. berghei kinesin-8B motor domain in AMPPNP state bound to tubulin dimer Deposited 2022-02-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–437（437 aa）
Not recorded ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7Z2C P. falciparum kinesin-8B motor domain in no nucleotide bound to tubulin dimer Deposited 2022-02-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–437（437 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8DIQ Tubulin-RB3_SLD-TTL in complex with SB226 Deposited 2022-06-29 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 CA CALCIUM ION × 2 MG MAGNESIUM ION × 5 DMS DIMETHYL SULFOXIDE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 JVI 4-[2-(ethylamino)-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl]-7-methoxy-3,4-dihydroquinoxalin-2(1H)-one × 2 GOL GLYCEROL × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG4K, MES, Glycerol, CaCl2, MgCl2
Resolution 2.40 Å R-free 0.209
8F18 Apo KIF20A[1-565] class-2 in complex with a microtubule Deposited 2022-11-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8F1A Apo KIF20A[1-565] class-1 in complex with a microtubule Deposited 2022-11-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8JJB Crystal structure of T2R-TTL-Y61 complex Deposited 2023-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 USI ~{N}4-(1~{H}-indol-5-ylmethyl)-6-(3-methoxyphenyl)pyrimidine-2,4-diamine × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 CL CHLORIDE ION × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, 30 mM MgCl2
Resolution 2.68 Å R-free 0.260
8JJC Tubulin-Y62 Deposited 2023-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 UPO 4-(6,7-dimethoxy-3,4-dihydro-1~{H}-isoquinolin-2-yl)-6-(3-methoxyphenyl)pyrimidin-2-amine × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 CL CHLORIDE ION × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, 30 mM MgCl2
Resolution 2.76 Å R-free 0.264
8RC1 MAP7 MTBD (microtubule binding domain) decorated microtubule protofilament Deposited 2023-12-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；80mM PIPES, 2mM MgCl2, 1mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE；Microtubules stabilised by MAP7 MTBD were applied to a glow discharged grid, incubated for 30 seconds at room temperature following which excess sample was wicked off and MAP7 MTBD was immediately added to the grid. After incubation for 60 seconds in a Vitrobot, excess liquid was blotted off and the grid was plunge frozen in liquid ethane.
Resolution 3.70 Å
8RHB Microtubule-associated kinesin-1 tail complex bound to AMPPNP, single-headed state Deposited 2023-12-15 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–451（451 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 + 5mM AMPPNP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RHH Microtubule-associated kinesin-1 tail complex bound to AMPPNP, two-headed state Deposited 2023-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: pentameric
Chain A 1–451（451 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 + 5mM AMPPNP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RIK Microtubule-associated kinesin-1 tail complex bound to ADP, single-headed state Deposited 2023-12-18 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: tetrameric
Chain A 1–451（451 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 + 5 mM ADP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8RIZ Microtubule-associated kinesin-1 tail complex bound to ADP, two-headed state Deposited 2023-12-19 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–451（451 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 + 5mM ADP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8UTN KIF1A[1-393] AMP-PNP bound two-heads-bound state in complex with a microtubule (class T23L1) Deposited 2023-10-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–451（451 aa）
Chain E 1–451（451 aa）
Chain S 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UTO KIF1A[1-393] AMP-PNP bound two-heads-bound state in complex with a microtubule - class T2L1 Deposited 2023-10-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–451（451 aa）
Chain E 1–451（451 aa）
Chain S 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8UTP KIF1A[1-393] - AMP-PNP two-heads-bound state in complex with a microtubule - class T3L1 Deposited 2023-10-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–451（451 aa）
Chain E 1–451（451 aa）
Chain S 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8UTQ KIF1A[1-393] AMP-PNP bound one-head-bound state in complex with a microtubule - class T1L02* Deposited 2023-10-31 Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–451（451 aa）
Chain E 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 3 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UTR KIF1A[1-393] ADP bound in complex with a microtubule Deposited 2023-10-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8UTS KIF1A[1-393] APO in complex with a microtubule Deposited 2023-10-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8UTT KIF1A[1-393] P305L mutant AMP-PNP bound two-heads-bound state in complex with a microtubule Deposited 2023-10-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–451（451 aa）
Chain E 1–451（451 aa）
Chain S 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UTU KIF1A[1-393] P305L mutant AMP-PNP bound one and two heads bound states merged, in complex with a microtubule Deposited 2023-10-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–451（451 aa）
Chain E 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 3 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8UTV KIF1A[1-393] P305L mutant ADP bound in complex with a microtubule Deposited 2023-10-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–451（451 aa）
Chain E 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8UTW KIF1A[1-393] P305L mutant APO in complex with a microtubule Deposited 2023-10-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8UTY KIF1A[1-393] P364L mutant AMP-PNP bound two-heads-bound state in complex with a microtubule Deposited 2023-10-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–451（451 aa）
Chain E 1–451（451 aa）
Chain S 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8V4K CCP5 in complex with microtubules class1 Deposited 2023-11-29 Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ZN ZINC ION × 1 GLU GLUTAMIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8V4L CCP5 in complex with microtubules class2 Deposited 2023-11-29 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 GLU GLUTAMIC ACID × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8V4M CCP5 in complex with microtubules class3 Deposited 2023-11-29 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 GLU GLUTAMIC ACID × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8WMO Crystal structure analysis of tubulin and heterocyclic podophyllotoxins complex for anticancer agents Deposited 2023-10-04 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–440（440 aa）
Chain C 1–440（440 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 EDO 1,2-ETHANEDIOL × 8 PEG DI(HYDROXYETHYL)ETHER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 WIW (5~{S},5~{a}~{R},8~{a}~{R},9~{R})-5-pyrimidin-2-ylsulfanyl-9-(3,4,5-trimethoxyphenyl)-5~{a},6,8~{a},9-tetrahydro-5~{H}-[2]benzofuro[5,6-f][1,3]benzodioxol-8-one × 1 CA CALCIUM ION × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；277 K；6% glycerol, 6% PEG4000, 30 mM MgCl2, 30 mM CaCl2, 100 mM MES-Na
Resolution 2.89 Å R-free 0.266
8WMU Structural basis of tubulin and heterocyclic podophyllotoxins complex for anticancer agents with dual-binding sites Deposited 2023-10-04 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–440（440 aa）
Chain C 1–440（440 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 3 CA CALCIUM ION × 2 GOL GLYCEROL × 1 PEG DI(HYDROXYETHYL)ETHER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 A1D55 (5~{S},5~{a}~{S},8~{a}~{R},9~{R})-5-(quinolin-6-ylamino)-9-(3,4,5-trimethoxyphenyl)-5~{a},6,8~{a},9-tetrahydro-5~{H}-[2]benzofuro[6,5-f][1,3]benzodioxol-8-one × 1 EDO 1,2-ETHANEDIOL × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；277 K；6% PEG4000, 6% glycerol, 30 mM MgCl2, 30 mM CaCl2, 100 mM MES-Na
Resolution 2.70 Å R-free 0.245
8YEM Tubulin-RB3_SLD-TTL in complex with compound 9 Deposited 2024-02-22 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–440（440 aa）
Chain C 1–440（440 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 A1D6D 4-(2-chloranyl-6-fluoranyl-quinazolin-4-yl)-7-methoxy-1,3-dihydroquinoxalin-2-one × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 2.74 Å R-free 0.284
8YER Tubulin-RB3_SLD-TTL in complex with compound 4 Deposited 2024-02-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–440（440 aa）
Chain C 1–440（440 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 MG MAGNESIUM ION × 3 A1D6E 6-fluoranyl-4-(6-methoxy-3,4-dihydro-2~{H}-quinolin-1-yl)-~{N}-methyl-quinazolin-2-amine × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 2.71 Å R-free 0.286
8YEU Tubulin-RB3_SLD-TTL in complex with compound 2NH2 Deposited 2024-02-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–440（440 aa）
Chain C 1–440（440 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 MG MAGNESIUM ION × 3 A1D6L 6-fluoranyl-4-(6-methoxy-3,4-dihydro-2~{H}-quinolin-1-yl)quinazolin-2-amine × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 3.05 Å R-free 0.268
8YRK Tubulin-Compound KY216: stathmin-like domain complex Deposited 2024-03-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 GOL GLYCEROL × 1 A1D62 (11R,16S)-11-(3,5-dichloro-4-methoxyphenyl)-4,7-dioxa-12,14-diazatetracyclo[8.7.0.03,8.012,16]heptadeca-1,3(8),9-triene-13,15-dione × 2 EDO 1,2-ETHANEDIOL × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291.15 K；PEG,magnesium choride,calcium chloride,Ethylene glycol,MES
Resolution 2.74 Å R-free 0.226
8YTX Tubulin-RB3-TTL in complex with compound SI9 Deposited 2024-03-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–440（440 aa）
Chain C 1–440（440 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 A1D68 2-chloranyl-~{N}-(4-methoxyphenyl)-~{N}-methyl-thieno[2,3-d]pyrimidin-4-amine × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 2.53 Å R-free 0.266
8YU9 Tubulin-RB3-TTL in complex with compound SI10 Deposited 2024-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–440（440 aa）
Chain C 1–440（440 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 MG MAGNESIUM ION × 3 A1D7A 4-(2-chloranylthieno[3,2-d]pyrimidin-4-yl)-7-methoxy-1,3-dihydroquinoxalin-2-one × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 3.25 Å R-free 0.286
8YUA Tubulin-RB3-TTL in complex with compound SI10 Deposited 2024-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–440（440 aa）
Chain C 1–440（440 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 A1D69 2-chloranyl-~{N}-(4-methoxyphenyl)-~{N}-methyl-thieno[3,2-d]pyrimidin-4-amine × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 2.37 Å R-free 0.254
8YY2 Kinesin-14 in nucleotide-free state bound to 13 PF Microtubule Deposited 2024-04-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；100 mM PIPES pH 6.8, 1 mM MgCl2, 1 mM EGTA, and 1 mM GTP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8YY3 Kinesin-14 in nucleotide-free state bound to 14 PF Microtubule Deposited 2024-04-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；100 mM PIPES pH 6.8, 1 mM MgCl2, 1 mM EGTA, and 1 mM GTP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.24 Å
8YY4 Kinesin-14 with AlF3 bound to 13 PF Microtubule Deposited 2024-04-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ALF TETRAFLUOROALUMINATE ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；100 mM PIPES pH 6.8, 1 mM MgCl2, 1 mM EGTA, 1 mM GTP, 2 mM ADP, 2 mM AlCl3, and 8 mM NaF
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.02 Å
8YY5 Kinesin-14 with AlF3 bound to 14 PF Microtubule Deposited 2024-04-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ALF TETRAFLUOROALUMINATE ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；100 mM PIPES pH 6.8, 1 mM MgCl2, 1 mM EGTA, 1 mM GTP, 2 mM ADP, 2 mM AlCl3, and 8 mM NaF
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.99 Å
8ZB8 Crystal structure of T2R-TTL-DPP21 complex Deposited 2024-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 CA CALCIUM ION × 2 GOL GLYCEROL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 A1D8I ~{N},2-dimethyl-~{N}-(1-methylindol-5-yl)thieno[3,2-d]pyrimidin-4-amine × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% polyethylene glycol 4000, 8% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.94 Å R-free 0.259
9DA8 Tau-Microtubule structure in the presence of ATP Deposited 2024-08-21 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–451（451 aa）
Chain L 1–451（451 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 4 TA1 TAXOL × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.94 Å
9DI0 Cryo-EM structure of Kif18A bound to a microtubule Deposited 2024-09-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric
Chain L 1–451（451 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 2 TA1 TAXOL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9DUQ HURP(65-174) bound to GMPCPP-stabilized microtubule Deposited 2024-10-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric
Chain A 1–439（439 aa）
Chain C 1–439（439 aa）
Chain E 1–439（439 aa）
Chain G 1–439（439 aa）
Chain I 1–439（439 aa）
Chain K 1–439（439 aa）
Chain M 1–439（439 aa）
Chain O 1–439（439 aa）
Chain Q 1–439（439 aa）
Not recorded G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 9 MG MAGNESIUM ION × 18 GTP GUANOSINE-5'-TRIPHOSPHATE × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 (1X): 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9DXC Model of tubulin dimers used for determining the dimer rise in a taxol-stabilized microtubule-HURP complex Deposited 2024-10-11 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9DXE Model of tubulin dimers used for determining the dimer rise in a taxol-stabilized microtubule Deposited 2024-10-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9GNQ Microtubule-associated Kif5B IAK tail mutant bound to ADP Deposited 2024-09-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 + 5mM AMPPNP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9IM5 Tubulin-RB3(MUT)-TTL-Y12 Deposited 2024-07-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 CL CHLORIDE ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 A1L2T ~{N}4-(1,3-benzodioxol-5-ylmethyl)-6-(1~{H}-indol-4-yl)pyrimidine-2,4-diamine × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1M MES, 30mM CaCl2, 30mM MgCl2, pH 6.7
Resolution 2.86 Å R-free 0.246
9IMO Crystal structure of Tubulin-RB3-TTL-Y12 Deposited 2024-07-04 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 5 CA CALCIUM ION × 3 A1L2T ~{N}4-(1,3-benzodioxol-5-ylmethyl)-6-(1~{H}-indol-4-yl)pyrimidine-2,4-diamine × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1M MES, 30mM CaCl2, 30mM MgCl2, pH 6.7
Resolution 2.75 Å R-free 0.255
9IXB Structure of tubulin and nitrogen-containing heterocyclic substituted podophyllotoxin derivatives complex Deposited 2024-07-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–440（440 aa）
Chain C 1–440（440 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 EDO 1,2-ETHANEDIOL × 1 MG MAGNESIUM ION × 3 CA CALCIUM ION × 1 NA SODIUM ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 A1ECQ (5~{S},5~{a}~{S},8~{a}~{R},9~{R})-5-(1~{H}-indazol-5-ylamino)-9-(3,4,5-trimethoxyphenyl)-5~{a},6,8~{a},9-tetrahydro-5~{H}-[2]benzofuro[6,5-f][1,3]benzodioxol-8-one × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 IMD IMIDAZOLE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；277 K；6% PEG4000, 6% glycerol, 30 mM MgCl2, 30 mM CaCl2, 100 mM MES-Na
Resolution 3.48 Å R-free 0.267
9K7E Crystal structure of T2R-TTL-taccalonolide Y8 complex Deposited 2024-10-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–440（440 aa）
Chain C 1–440（440 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 MG MAGNESIUM ION × 3 A1EE1 (4~{R})-5-(4-hydroxyphenyl)-3-(4-methylphenyl)-4-phenyl-2,4-dihydropyrrolo[3,4-c]pyrazol-6-one × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.45 Å R-free 0.267
9KBL Crystal structure of T2R-TTL-IKP104 Deposited 2024-10-31 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 7 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 W4Q 1-(2-chloranyl-3,5-dimethoxy-phenyl)-2-(4-fluorophenyl)-3-methyl-6-phenyl-pyridin-4-one × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2 and 30 mM MgCl2.
Resolution 3.14 Å R-free 0.238
9KBN Crystal structure of T2R-TTL-IKP104-Colchicine Deposited 2024-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 7 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 W4Q 1-(2-chloranyl-3,5-dimethoxy-phenyl)-2-(4-fluorophenyl)-3-methyl-6-phenyl-pyridin-4-one × 1 LOC N-[(7S)-1,2,3,10-tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[d]heptalen-7-yl]ethanamide × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2 and 30 mM MgCl2.
Resolution 2.56 Å R-free 0.262
9KBW CryoEM structure of microtubule bound with GAS2-GAR domain. Deposited 2024-10-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1–439（439 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；80 mM PIPES, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9L7M Nucleotide-free kinesin-1 motor domain bound to the microtubule Deposited 2024-12-26 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
9LNL Crystal structure of T2R-TTL-YQVB15 complex Deposited 2025-01-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 MG MAGNESIUM ION × 2 A1EPQ 10',11'-difluoro-12'-methoxyvinblastine × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.85 Å R-free 0.277
9LNV Crystal structure of T2R-TTL-YQVB6 Complex Deposited 2025-01-22 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 CA CALCIUM ION × 2 A1EPR 10'-fluorovinblastine × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.67 Å R-free 0.288
9LNW Crystal structure of T2R-TTL-YQVB8 Complex Deposited 2025-01-22 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 A1EPS 10'-bromovinblastine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.55 Å R-free 0.292
9LNX Crystal structure of T2R-TTL-YQVB9 Complex Deposited 2025-01-22 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–450（450 aa）
Chain C 1–450（450 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 CA CALCIUM ION × 2 A1EPT 10'-methoxyvinblastine × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7
Resolution 2.59 Å R-free 0.296
9M1L Cryo-EM structure of the TBC-DE-Arl2-alpha-beta-tubulin complex with GTP Deposited 2025-02-26 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain a 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.55 Å
9M1M Cryo-EM structure of the TBC-DEC-Arl2-alpha-beta-tubulin complex with GDP-AlFx Deposited 2025-02-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain a 1–451（451 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 AF3 ALUMINUM FLUORIDE × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.21 Å
9M1N Cryo-EM structure of the TBC-DC-Arl2-alpha-beta-tubulin complex with GDP-AlFx Deposited 2025-02-26 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain a 1–451（451 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 AF3 ALUMINUM FLUORIDE × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.24 Å
9MLF TUBULIN-RB3_SLD IN COMPLEX WITH COMPOUND QW-4-183-llb Deposited 2024-12-19 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–438（438 aa）
Chain C 1–438（438 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 A1BMG [(4M)-4-(1H-indol-4-yl)-1H-imidazol-2-yl](3,4,5-trimethoxyphenyl)methanone × 2 SO4 SULFATE ION × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.6；293.15 K；0.1 M trisodium citrate, pH 5.6, 0.2 M ammonium sulfate, 12.2% PEG 4000
Resolution 2.40 Å R-free 0.233
9N9E Cryo-EM structure of the SAH domain of Caenorhabditis elegans ICP-1 bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
9N9F Cryo-EM structure of the SAH domain of human INCENP bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.91 Å
9N9G Cryo-EM structure of the human Hec1-Nuf2 dimer bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 1–451（451 aa）
Chain a 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9N9H Cryo-EM structure of the Xenopus laevis mitotic centromere-associated kinesin (MCAK) bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–451（451 aa）
Chain a 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.31 Å
9N9I Cryo-EM structure of the Xenopus laevis mitotic centromere-associated kinesin (MCAK) bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain 1 1–451（451 aa）
Chain 4 1–451（451 aa）
Chain A 1–451（451 aa）
Chain a 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 TA1 TAXOL × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9NNL Bordetella filamentous hemagglutinin (FhaB) C-terminal domain bound to microtubules Deposited 2025-03-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–451（451 aa）
Chain E 1–451（451 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 4 TA1 TAXOL × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.42 Å
9O6I TUBULIN-RB3_SLD IN COMPLEX WITH COMPOUND QW-5-70 Deposited 2025-04-13 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–438（438 aa）
Chain C 1–438（438 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 A1B97 [(4M)-4-(1H-indazol-4-yl)-1H-imidazol-2-yl](3,4,5-trimethoxyphenyl)methanone × 2 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.6；293.15 K；0.1 M trisodium citrate, pH 5.6, 0.2 M ammonium sulfate, 12.2% PEG 4000
Resolution 2.51 Å R-free 0.244
9U6A Tubulin-DARPin D1 in complex with a flavone Deposited 2025-03-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 A1L8X 3-methoxy-2-(4-methoxy-3-oxidanyl-phenyl)-5,7-bis(oxidanyl)chromen-4-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；PEG 3000, ammonium sulfate, bis-tris methane
Resolution 1.92 Å R-free 0.224
9U6D Crystal structure of tubulin-RB3-TTL in complex with X8 Deposited 2025-03-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 A1L8Z 2-[2-fluoranyl-4-[4-[2-[4-[(1~{R},2~{S})-2-fluoranylcyclopropyl]carbonylpiperazin-1-yl]ethoxy]phenyl]phenyl]-~{N}-(phenylmethyl)ethanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1M MES, 30mM CaCl2, 30mM MgCl2, pH 6.7
Resolution 2.54 Å R-free 0.238
9U9Q Tubulin-RB3-TTL in complex with C38 Deposited 2025-03-29 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 7 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 A1L80 2-(1-benzofuran-5-yl)-6-[2,6-bis(fluoranyl)-4-[3-(methylamino)propoxy]phenyl]-5-chloranyl-~{N}-[(2~{S})-1,1,1-tris(fluoranyl)propan-2-yl]-[1,2,4]triazolo[1,5-a]pyrimidin-7-amine × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1M MES, 30mM CaCl2, 30mM MgCl2, pH 6.7
Resolution 2.93 Å R-free 0.269
9UMU Giraffe KIF5A motor domain in nucleotide free state bound to microtubule Deposited 2025-04-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
9YA5 KIF1A R350G bound to microtubules in two-heads-bound state with AMP-PNP Deposited 2025-09-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain E 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 buffer composed of 80 mM PIPES, 2 mM MgCl2, 1 mM EGTA, PH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；4uL of a 6uM MT solution in BRB80 (80mM PIPES, 2mM MgCl2, 1mM EGTA pH 6.8) along with 20uM paclitaxel was added onto a plasma-cleaned grid. The MTs were incubated for 1 minute at room temperature, and then the excess liquid was removed from the grid using Whatman #1 paper. Next, 4uL of a solution containing 20uM KIF1A-R350G in BRB80 supplemented with 20uM paclitaxel and 5mM AMP-PNP was added to the grid. The grid with the MT and kinesin mixture was then mounted into a Vitrobot, incubated for 1 minute at room temperature, and plunge-frozen into liquid nitrogen-cooled ethane.
Resolution 2.95 Å
9YA7 KIF1A R350G bound to microtubules in the apo state Deposited 2025-09-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 buffer composed of 80 mM PIPES, 2 mM MgCl2, 1 mM EGTA, PH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；4uL of a 6uM MT solution in BRB80 (80mM PIPES, 2mM MgCl2, 1mM EGTA pH 6.8) along with 20uM paclitaxel was added onto a plasma-cleaned grid. The MTs were incubated for 1 minute at room temperature, and then the excess liquid was removed from the grid using Whatman #1 paper. Next, 4uL of a solution containing 20uM KIF1A-R350G in BRB80 supplemented with 20uM paclitaxel and 0.005 units per uL apyrase was added to the grid. The grid with the MT and kinesin mixture was then mounted into a Vitrobot, incubated for 1 minute at room temperature, and plunge-frozen into liquid nitrogen-cooled ethane.
Resolution 3.29 Å
9YAB KIF1A R350W bound to microtubules in the apo state Deposited 2025-09-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 buffer composed of 80 mM PIPES, 2 mM MgCl2, 1 mM EGTA, PH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；4uL of a 6uM MT solution in BRB80 (80mM PIPES, 2mM MgCl2, 1mM EGTA pH 6.8) along with 20uM paclitaxel was added onto a plasma-cleaned grid. The MTs were incubated for 1 minute at room temperature, and then the excess liquid was removed from the grid using Whatman #1 paper. Next, 4uL of a solution containing 17.5uM KIF1A-R350W in BRB80 supplemented with 20uM paclitaxel and 0.005 units per uL apyrase was added to the grid. The grid with the MT and kinesin mixture was then mounted into a Vitrobot, incubated for 1 minute at room temperature, and plunge-frozen into liquid nitrogen-cooled ethane.
Resolution 3.21 Å
9YAI KIF1A R350W bound to microtubules in two-heads-bound state with AMP-PNP Deposited 2025-09-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain E 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 3 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 buffer composed of 80 mM PIPES, 2 mM MgCl2, 1 mM EGTA, PH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；4uL of a 6uM MT solution in BRB80 (80mM PIPES, 2mM MgCl2, 1mM EGTA pH 6.8) along with 20uM paclitaxel was added onto a plasma-cleaned grid. The MTs were incubated for 1 minute at room temperature, and then the excess liquid was removed from the grid using Whatman #1 paper. Next, 4uL of a solution containing 17.5uM KIF1A-R350W in BRB80 supplemented with 20uM paclitaxel and 5mM AMP-PNP was added to the grid. The grid with the MT and kinesin mixture was then mounted into a Vitrobot, incubated for 1 minute at room temperature, and plunge-frozen into liquid nitrogen-cooled ethane.
Resolution 3.12 Å
9YMG Human KIF18A-DARPin fusion protein bound to AMP-PNP and tubulin Deposited 2025-10-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 EDO 1,2-ETHANEDIOL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.2 M ammonium nitrate, 0.1 M sodium acetate pH 5.6, 20% (w/v) PEG Smear Low (5% PEG 400, 5% PEG 500 MME, 5% PEG 600, 5% PEG 1000), 5% (v/v) ethylene glycol
Resolution 2.41 Å R-free 0.238
9YMG Human KIF18A-DARPin fusion protein bound to AMP-PNP and tubulin Deposited 2025-10-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain D 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.2 M ammonium nitrate, 0.1 M sodium acetate pH 5.6, 20% (w/v) PEG Smear Low (5% PEG 400, 5% PEG 500 MME, 5% PEG 600, 5% PEG 1000), 5% (v/v) ethylene glycol
Resolution 2.41 Å R-free 0.238
9YXQ Cryo-EM structure of the Xenopus laevis mitotic centromere-associated kinesin (MCAK) bound to the paclitaxel-stabilized microtubule Deposited 2025-10-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: 14-meric
Chain 1 1–451（451 aa）
Chain 3 1–451（451 aa）
Chain 6 1–451（451 aa）
Chain 8 1–451（451 aa）
Chain A 1–451（451 aa）
Chain a 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 11 GTP GUANOSINE-5'-TRIPHOSPHATE × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 TA1 TAXOL × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.39 Å

230 other PDB entries and 243 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name TBA1B_PIG
Isoform —
PDB entities 3
Chains and sequence ranges Author chain B； PDBConstruct 1–451； UniProt 1–451 Author chain L； PDBConstruct 1–451； UniProt 1–451

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 9dhz

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 9dhz
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2. Structure Basics 2. Structure Basics

Entry ID entry_id9dhz
Deposition date deposition_date2024-09-04
最后修订 last_revision2025-07-16
Structure title titleCryo-EM structure of HURP bound to a microtubule
Keywords keywordsHURP, Tubulin, K-fiber, mitosis, spindle, PROTEIN BINDING; PROTEIN BINDING
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier40.20 Å
Radius of gyration Rg (electron density) rg_electron39.70 Å
Forward intensity I(0) i0631216000.00
Molecular weight molecular_weight201630.0 kDa
Excluded volume excluded_volume250060 ų
Envelope volume envelope_volume319000 ų
Hydration-shell volume shell_volume64765 ų
Envelope diameter envelope_diameter137.4 Å
Shell Rg shell_rg47.19 Å
Envelope Rg envelope_rg39.34 Å
Shape Rg shape_rg39.71 Å
Total Rg total_rg40.04 Å
Total atoms total_atoms14150
Residues n_residues1768
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax128.5 Å
Rg (real space) rg_real40.12 Å
Rg uncertainty (real space) rg_real_error1.21 Å
I(0) (real space) i0_real6.3120e+08
I(0) uncertainty (real space) i0_real_error1.2610e+07
Rg (reciprocal space) rg_reciprocal40.20 Å
I(0) (reciprocal space) i0_reciprocal631300000.0000
Solution quality estimate total_estimate0.8948
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary46.1 Å
Skewness Skewness skewness0.222
Kurtosis Kurtosis kurtosis-0.506
Angular range angular_range— – 0.1950 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha211000000.0000
Real-space data points n_real_points40
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.921; Stabil: 0.998; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.874
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (7)

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8. Citations (1)

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9. Files and Curves (10)