9mmu

M5 protein with Factor H 6-7 domain

Method: X-RAY DIFFRACTION D_max: 127.4 Å Quality: GOOD

▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

M protein, serotype 5

Streptococcus pyogenes str. Manfredo

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count	Chain A； UniProt 94–175 Chain B； UniProt 94–175	Not recorded	Complement factor H × 2 （P08603）	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；291 K；PEG 6000, Ethylene glycol, Sodium Chloride	Resolution 2.25 Å R-free 0.287
2	Protein heterocomplex Heteromer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count	Chain E； UniProt 94–175 Chain F； UniProt 94–175	Not recorded	Complement factor H × 2 （P08603）	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；291 K；PEG 6000, Ethylene glycol, Sodium Chloride	Resolution 2.25 Å R-free 0.287

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 9MMU	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
2KK9 Anti-group A streptococcal vaccine epitope: structure, stability and its ability to interact with HLA class II molecules Deposited 2009-06-16	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 300–354（55 aa） Fragment：UNP residues 300-354	Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 3.7；295 K；Pressure ambient NMR sample composition 5.4 mg/mL, trifluoroethanol/water 30/70% \| trifluoroethanol/water 30/70%	Resolution not provided

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	M5_STRP5
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 5–86； UniProt 94–175 Author chain B； PDBConstruct 5–86； UniProt 94–175 Author chain E； PDBConstruct 5–86； UniProt 94–175 Author chain F； PDBConstruct 5–86； UniProt 94–175

Complement factor H

Homo sapiens

UniProt P08603

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count	Chain C； UniProt 321–443 Chain D； UniProt 321–443	Not recorded	M protein, serotype 5 × 2 （P02977）	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；291 K；PEG 6000, Ethylene glycol, Sodium Chloride	Resolution 2.25 Å R-free 0.287
2	Protein heterocomplex Heteromer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count	Chain G； UniProt 321–443 Chain H； UniProt 321–443	Not recorded	M protein, serotype 5 × 2 （P02977）	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；291 K；PEG 6000, Ethylene glycol, Sodium Chloride	Resolution 2.25 Å R-free 0.287

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 9MMU	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
1HAQ FOUR MODELS OF HUMAN FACTOR H DETERMINED BY SOLUTION SCATTERING CURVE-FITTING AND HOMOLOGY MODELLING Deposited 2001-04-06	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 19–1231（1213 aa）	Not recorded	No recorded non-water small molecule	SOLUTION SCATTERING mmCIF provides none of the parsed conditions	Resolution not provided
1HCC THREE-DIMENSIONAL STRUCTURE OF A COMPLEMENT CONTROL PROTEIN MODULE IN SOLUTION Deposited 1990-11-28	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 927–985（59 aa）	Not recorded	No recorded non-water small molecule	SOLUTION NMR mmCIF provides none of the parsed conditions	Resolution not provided
1HFH SOLUTION STRUCTURE OF A PAIR OF COMPLEMENT MODULES BY NUCLEAR MAGNETIC RESONANCE Deposited 1993-02-23	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 866–985（120 aa）	Not recorded	No recorded non-water small molecule	SOLUTION NMR mmCIF provides none of the parsed conditions	Resolution not provided
1HFI SOLUTION STRUCTURE OF A PAIR OF COMPLEMENT MODULES BY NUCLEAR MAGNETIC RESONANCE Deposited 1993-02-23	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 866–927（62 aa）	Not recorded	No recorded non-water small molecule	SOLUTION NMR mmCIF provides none of the parsed conditions	Resolution not provided
2BZM Solution structure of the primary host recognition region of complement factor H Deposited 2005-08-18	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1107–1231（125 aa） Fragment：CCPS 19-20, RESIDUES 1107-1231	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 4；310 K；Ionic strength (raw mmCIF value) 200；Pressure 1.0 NMR sample composition 10% WATER/90% D2O	Resolution not provided
2G7I Structure of Human Complement Factor H Carboxyl Terminal Domains 19-20: a Basis for Atypical Hemolytic Uremic Syndrome Deposited 2006-02-28	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1107–1231（125 aa） Fragment：C-terminal Domains 19-20	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；Before crystallization the protein was concentrated to 10 mg/ml and dialysed into 20 mM Tris, 50 mM NaCl, pH 7.0. The protein was crystallized in sitting drops by mixing 1 ul of protein solution at 10 mg/ml with 1 ul of reservoir solution of 2.2 M (NH4)2SO4, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K	Resolution 1.75 Å R-free 0.225
2IC4 Solution structure of the His402 allotype of the Factor H SCR6-SCR7-SCR8 fragment Deposited 2006-09-12	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 321–506（186 aa） Fragment：Factor H SCR6-SCR7-SCR8	Not recorded	No recorded non-water small molecule	SOLUTION SCATTERING X-ray crystallization conditions pH 7.3；288 K；SOLUTION, pH 7.3, temperature 288K	Resolution not provided
2JGW Structure of CCP module 7 of complement factor H - The AMD at risk varient (402H) Deposited 2007-02-16	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 386–446（61 aa） Fragment：CCP MODULE 7, RESIDUES 386-446	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 5.2；298 K；Ionic strength (raw mmCIF value) 20；Pressure 1.0 NMR measurement conditions pH 5.2；298 K；Ionic strength (raw mmCIF value) 20；Pressure 1.0 NMR sample composition 10% WATER/90% D2O	Resolution not provided
2JGX Structure of CCP module 7 of complement factor H - The AMD Not at risk varient (402Y) Deposited 2007-02-16	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 386–446（61 aa） Fragment：CCP MODULE 7, RESIDUES 386-446	Mutation：YES	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 5.2；298 K；Ionic strength (raw mmCIF value) 20；Pressure 1.0 NMR measurement conditions pH 5.2；298 K；Ionic strength (raw mmCIF value) 20；Pressure 1.0 NMR sample composition 10% WATER/90% D2O	Resolution not provided
2KMS Combined high- and low-resolution techniques reveal compact structure in central portion of factor H despite long inter-modular linkers Deposited 2009-08-04	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 690–804（115 aa） Fragment：Sushi domain, residues 690-804	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 6.6；298 K；Ionic strength (raw mmCIF value) 20；Pressure ambient NMR sample composition 20mM potassium phosphate-1, 93% H2O/7% D2O \| 93% H2O/7% D2O	Resolution not provided
2QFG Solution Structure of the N-terminal SCR-1/5 fragment of Complement Factor H. Deposited 2007-06-27	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 19–322（304 aa） Fragment：SCR domains 1-5 (residues 19-322)	Not recorded	No recorded non-water small molecule	SOLUTION SCATTERING mmCIF provides none of the parsed conditions	Resolution not provided
2QFH Solution Structure of the C-terminal SCR-16/20 fragment of Complement Factor H. Deposited 2007-06-27	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 928–1231（304 aa） Fragment：SCR domains 16-20 (residues 928-1231)	Not recorded	No recorded non-water small molecule	SOLUTION SCATTERING mmCIF provides none of the parsed conditions	Resolution not provided
2RLP NMR structure of CCP modules 1-2 of complement factor H Deposited 2007-07-28	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 20–142（123 aa） Fragment：residues in database 20-142	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 6.2；310 K；Pressure ambient NMR sample composition 1mM [U-95% 13C; U-95% 15N] FactorH CCP1-2, 10mM potassium phosphate, 12mg/mL Pf1 phage, 50mM L-Arg, 50mM L-Glu, 90% H2O/10% D2O \| 90% H2O/10% D2O NMR sample composition 1mM FactorH CCP1-2, 10mM potassium phosphate, 50mM L-Arg, 50mM L-Glu, 90% H2O/10% D2O \| 90% H2O/10% D2O NMR sample composition 1mM FactorH CCP1-2, 10mM potassium phosphate, 50mM L-Arg, 50mM L-Glu, 100% D2O \| 100% D2O	Resolution not provided
2RLQ NMR structure of CCP modules 2-3 of complement factor H Deposited 2007-07-29	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 84–206（123 aa） Fragment：residues in database 84-206	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 6.2；310 K；Pressure ambient NMR sample composition 2mM [U-95% 13C; U-95% 15N] FactorH CCP2-3, 10mM potassium phosphate, 12mg/mL Pf1 phage, 50mM L-Arg, 50mM L-Glu, 90% H2O/10% D2O \| 90% H2O/10% D2O NMR sample composition 3mM FactorH CCP2-3, 10mM potassium phosphate, 50mM L-Arg, 50mM L-Glu, 90% H2O/10% D2O \| 90% H2O/10% D2O NMR sample composition 1mM FactorH CCP2-3, 10mM potassium phosphate, 50mM L-Arg, 50mM L-Glu, 100% D2O \| 100% D2O	Resolution not provided
2UWN Crystal structure of Human Complement Factor H, SCR domains 6-8 (H402 risk variant), in complex with ligand. Deposited 2007-03-22	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric	Chain A 322–506（185 aa） Fragment：DOMAINS 6,7 AND 8, RESIDUES 322-506	Not recorded	ACT ACETATE ION × 1 CL CHLORIDE ION × 2 SO4 SULFATE ION × 3	X-RAY DIFFRACTION X-ray crystallization conditions pH 4.5；0.1M SODIUM ACETATE, PH4.5, 20% (W/V) PEG3000	Resolution 2.35 Å R-free 0.246
2V8E Crystal structure of Human Complement Factor H, SCR domains 6-8 (H402 risk variant), in complex with ligand. Deposited 2007-08-07	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric	Chain A 322–506（185 aa） Fragment：DOMAINS 6,7 AND 8, RESIDUES 322-506	Non-standard monomer：Yes (specific site not provided by mmCIF)	CL CHLORIDE ION × 2 SO4 SULFATE ION × 3 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 4.5；0.1M SODIUM ACETATE, PH4.5, 20% (W/V) PEG3000	Resolution 2.50 Å R-free 0.245
2W80 Structure of a complex between Neisseria meningitidis factor H binding protein and CCPs 6-7 of human complement factor H Deposited 2009-01-08	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 321–443（123 aa） Fragment：CCPS 6 AND 7, RESIDUES 321-443	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 9；20% PEG 6000, 0.1M BICINE PH9.0	Resolution 2.35 Å R-free 0.263
2W80 Structure of a complex between Neisseria meningitidis factor H binding protein and CCPs 6-7 of human complement factor H Deposited 2009-01-08	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 321–443（123 aa） Fragment：CCPS 6 AND 7, RESIDUES 321-443	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 9；20% PEG 6000, 0.1M BICINE PH9.0	Resolution 2.35 Å R-free 0.263
2W80 Structure of a complex between Neisseria meningitidis factor H binding protein and CCPs 6-7 of human complement factor H Deposited 2009-01-08	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain E 321–443（123 aa） Fragment：CCPS 6 AND 7, RESIDUES 321-443	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 9；20% PEG 6000, 0.1M BICINE PH9.0	Resolution 2.35 Å R-free 0.263
2W80 Structure of a complex between Neisseria meningitidis factor H binding protein and CCPs 6-7 of human complement factor H Deposited 2009-01-08	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain G 321–443（123 aa） Fragment：CCPS 6 AND 7, RESIDUES 321-443	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 9；20% PEG 6000, 0.1M BICINE PH9.0	Resolution 2.35 Å R-free 0.263
2W81 Structure of a complex between Neisseria meningitidis factor H binding protein and CCPs 6-7 of human complement factor H Deposited 2009-01-08	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 321–443（123 aa） Fragment：CCPS 6 AND 7, RESIDUES 321-443	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 9；20% PEG 6000, 0.1M BICINE PH 9.0	Resolution 2.35 Å R-free 0.283
2W81 Structure of a complex between Neisseria meningitidis factor H binding protein and CCPs 6-7 of human complement factor H Deposited 2009-01-08	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 321–443（123 aa） Fragment：CCPS 6 AND 7, RESIDUES 321-443	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 9；20% PEG 6000, 0.1M BICINE PH 9.0	Resolution 2.35 Å R-free 0.283
2W81 Structure of a complex between Neisseria meningitidis factor H binding protein and CCPs 6-7 of human complement factor H Deposited 2009-01-08	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain E 321–443（123 aa） Fragment：CCPS 6 AND 7, RESIDUES 321-443	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 9；20% PEG 6000, 0.1M BICINE PH 9.0	Resolution 2.35 Å R-free 0.283
2WII Complement C3b in complex with factor H domains 1-4 Deposited 2009-05-12	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric	Chain C 18–264（247 aa） Fragment：RESIDUES 18-264	Not recorded	CA CALCIUM ION × 1 GOL GLYCEROL × 17 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.1；7.0% (W/V) PEG 3,350, 70 MM AMMONIUM ACETATE, PH 7.1	Resolution 2.70 Å R-free 0.252
2WII Complement C3b in complex with factor H domains 1-4 Deposited 2009-05-12	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain C 18–264（247 aa） Fragment：RESIDUES 18-264	Not recorded	GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.1；7.0% (W/V) PEG 3,350, 70 MM AMMONIUM ACETATE, PH 7.1	Resolution 2.70 Å R-free 0.252
2XQW Structure of Factor H domains 19-20 in complex with complement C3d Deposited 2010-09-07	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain C 1103–1231（129 aa） Fragment：DOMAINS 19-20, RESIDUES 1103-1231	Mutation：YES	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.5；295 K；12-18% PEG 4000, 0.1 M HEPES, PH 7.5, AT 22 DEGREES C	Resolution 2.31 Å R-free 0.242
2XQW Structure of Factor H domains 19-20 in complex with complement C3d Deposited 2010-09-07	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain C 1103–1231（129 aa） Fragment：DOMAINS 19-20, RESIDUES 1103-1231	Mutation：YES	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.5；295 K；12-18% PEG 4000, 0.1 M HEPES, PH 7.5, AT 22 DEGREES C	Resolution 2.31 Å R-free 0.242
3GAU Solution structure of Human Complement Factor H in 50 mM NaCl buffer Deposited 2009-02-18	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 19–1231（1213 aa）	Not recorded	No recorded non-water small molecule	SOLUTION SCATTERING mmCIF provides none of the parsed conditions	Resolution not provided
3GAV Solution structure of Human Complement Factor H in 137 mM NaCl buffer Deposited 2009-02-18	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 19–1231（1213 aa）	Not recorded	No recorded non-water small molecule	SOLUTION SCATTERING mmCIF provides none of the parsed conditions	Resolution not provided
3GAW Solution structure of Human Complement Factor H in 250 mM NaCl buffer Deposited 2009-02-18	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 19–1231（1213 aa）	Not recorded	No recorded non-water small molecule	SOLUTION SCATTERING mmCIF provides none of the parsed conditions	Resolution not provided
3KXV Structure of complement Factor H variant Q1139A Deposited 2009-12-04	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1103–1231（129 aa） Fragment：Sushi domains 19-20	Mutation：Q1139A	SO4 SULFATE ION × 24	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；295.15 K；2M ammonium sulphate, 0.1M Bis-Tris buffer, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295.15K	Resolution 2.00 Å R-free 0.244
3KZJ Structure of complement Factor H variant R1203A Deposited 2009-12-08	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1103–1231（129 aa） Fragment：Sushi domains 19-20	Mutation：R1203A	SO4 SULFATE ION × 8	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；295.15 K；2M Ammonium Sulphate, 0.1M Bis-Tris buffer, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295.15K	Resolution 1.65 Å R-free 0.222
3R62 Structure of complement regulator Factor H mutant, T1184R. Deposited 2011-03-21	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1107–1231（125 aa） Fragment：Complement Factor H modules 19 and 20	Mutation：T1184R	GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；290 K；0.1M Tris HCl, pH 8.5 and 12% polyethylene glycol 8000 , VAPOR DIFFUSION, HANGING DROP, temperature 290K	Resolution 1.52 Å R-free 0.208
3R62 Structure of complement regulator Factor H mutant, T1184R. Deposited 2011-03-21	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 1107–1231（125 aa） Fragment：Complement Factor H modules 19 and 20	Mutation：T1184R	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；290 K；0.1M Tris HCl, pH 8.5 and 12% polyethylene glycol 8000 , VAPOR DIFFUSION, HANGING DROP, temperature 290K	Resolution 1.52 Å R-free 0.208
3SW0 Structure of the C-terminal region (modules 18-20) of complement regulator Factor H Deposited 2011-07-13	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain X 1046–1231（186 aa） Fragment：unp residues 1046-1231	Not recorded	GOL GLYCEROL × 4 PO4 PHOSPHATE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.4；290 K；0.1M Sodium Acetate trihydrate, pH 4.6, 2% v/v Tacsimate, pH 4.0 (Hampton Research formulation containing: 1.8305 M Malonic acid, 0.25 M Ammonium citrate tribasic, 0.12 M Succinic acid, 0.3 M DL-Malic acid, 0.4 M Sodium acetate trihydrate, 0.5 M Sodium formate, 0.16 M Ammonium tartrate dibasic and pH adjusted using NaOH) and 16% w/v polyethylene glycol 3350 as the precipitant, VAPOR DIFFUSION, HANGING DROP, temperature 290K	Resolution 1.80 Å R-free 0.226
4AYD Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 1 R106A mutant Deposited 2012-06-20	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 321–443（123 aa） Fragment：CCPS 6 AND 7, RESIDUES 321-443	Not recorded	EDO 1,2-ETHANEDIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 9；20% PEG 6000, 0.1M BICINE PH 9.0	Resolution 2.40 Å R-free 0.231
4AYD Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 1 R106A mutant Deposited 2012-06-20	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 321–443（123 aa） Fragment：CCPS 6 AND 7, RESIDUES 321-443	Not recorded	EDO 1,2-ETHANEDIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 9；20% PEG 6000, 0.1M BICINE PH 9.0	Resolution 2.40 Å R-free 0.231
4AYD Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 1 R106A mutant Deposited 2012-06-20	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain E 321–443（123 aa） Fragment：CCPS 6 AND 7, RESIDUES 321-443	Not recorded	EDO 1,2-ETHANEDIOL × 2	X-RAY DIFFRACTION X-ray crystallization conditions pH 9；20% PEG 6000, 0.1M BICINE PH 9.0	Resolution 2.40 Å R-free 0.231
4AYE Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 1 E283AE304A mutant Deposited 2012-06-20	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 321–443（123 aa） Fragment：CCPS 6 AND 7, RESIDUES 321-443	Not recorded	EDO 1,2-ETHANEDIOL × 7	X-RAY DIFFRACTION X-ray crystallization conditions pH 9；20% PEG 6000, 0.1M BICINE PH 9.0	Resolution 2.80 Å R-free 0.226
4AYE Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 1 E283AE304A mutant Deposited 2012-06-20	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 321–443（123 aa） Fragment：CCPS 6 AND 7, RESIDUES 321-443	Not recorded	EDO 1,2-ETHANEDIOL × 7	X-RAY DIFFRACTION X-ray crystallization conditions pH 9；20% PEG 6000, 0.1M BICINE PH 9.0	Resolution 2.80 Å R-free 0.226
4AYE Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 1 E283AE304A mutant Deposited 2012-06-20	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain E 321–443（123 aa） Fragment：CCPS 6 AND 7, RESIDUES 321-443	Not recorded	EDO 1,2-ETHANEDIOL × 5	X-RAY DIFFRACTION X-ray crystallization conditions pH 9；20% PEG 6000, 0.1M BICINE PH 9.0	Resolution 2.80 Å R-free 0.226
4AYI Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 3 Wild type Deposited 2012-06-21	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 321–443（123 aa） Fragment：CCPS 6 AND 7, RESIDUES 321-443	Not recorded	EDO 1,2-ETHANEDIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 6；0.2 M IMIDAZOLE PH 6 20% PEG 4000	Resolution 2.31 Å R-free 0.252
4AYI Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 3 Wild type Deposited 2012-06-21	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain E 321–443（123 aa） Fragment：CCPS 6 AND 7, RESIDUES 321-443	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 6；0.2 M IMIDAZOLE PH 6 20% PEG 4000	Resolution 2.31 Å R-free 0.252
4AYM Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 3 P106A mutant Deposited 2012-06-21	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 321–443（123 aa） Fragment：CCPS 6 AND 7, RESIDUES 321-443	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 6；0.2 M IMIDAZOLE PH 6, 20% PEG 4000	Resolution 3.00 Å R-free 0.257
4AYM Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 3 P106A mutant Deposited 2012-06-21	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 321–443（123 aa） Fragment：CCPS 6 AND 7, RESIDUES 321-443	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 6；0.2 M IMIDAZOLE PH 6, 20% PEG 4000	Resolution 3.00 Å R-free 0.257
4AYM Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 3 P106A mutant Deposited 2012-06-21	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain E 321–443（123 aa） Fragment：CCPS 6 AND 7, RESIDUES 321-443	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 6；0.2 M IMIDAZOLE PH 6, 20% PEG 4000	Resolution 3.00 Å R-free 0.257
4AYM Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 3 P106A mutant Deposited 2012-06-21	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain F 321–443（123 aa） Fragment：CCPS 6 AND 7, RESIDUES 321-443	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 6；0.2 M IMIDAZOLE PH 6, 20% PEG 4000	Resolution 3.00 Å R-free 0.257
4B2R Solution structure of CCP modules 10-11 of complement factor H Deposited 2012-07-17	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 566–687（122 aa） Fragment：CCPS 10-11, RESIDUES 566-687	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 6.7；298 K；Ionic strength (raw mmCIF value) 0.02；Pressure 1.0 NMR measurement conditions pH 6.7；298 K；Ionic strength (raw mmCIF value) 0.02；Pressure 1.0 NMR measurement conditions pH 6.7；298 K；Ionic strength (raw mmCIF value) 0.02；Pressure 1.0 NMR measurement conditions pH 6.7；298 K；Ionic strength (raw mmCIF value) 0.02；Pressure 1.0 NMR sample composition 90% H2O/10% D2O NMR sample composition 90% H2O/10% D2O 90% H2O/10% D2O NMR sample composition 90% H2O/10% D2O	Resolution not provided
4B2S Solution structure of CCP modules 11-12 of complement factor H Deposited 2012-07-17	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 627–747（121 aa） Fragment：CCPS 11-12, RESIDUES 627-747	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 6.3；298 K；Ionic strength (raw mmCIF value) 0.02；Pressure 1.0 NMR measurement conditions pH 6.3；298 K；Ionic strength (raw mmCIF value) 0.02；Pressure 1.0 NMR sample composition 90% H2O/10% D2O	Resolution not provided
4J38 Structure of Borrelia burgdorferi Outer surface protein E in complex with Factor H domains 19-20 Deposited 2013-02-05	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 1103–1231（129 aa） Fragment：FH domains 19-20, UNP residues 1103-1231	Mutation：D1119G, Q1139A	SO4 SULFATE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；293 K；2M Ammonium sulfate, 0.1M Citric acid, 0.2M Sodium chloride, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 2.83 Å R-free 0.255
4K12 Structural Basis for Host Specificity of Factor H Binding by Streptococcus pneumoniae Deposited 2013-04-04	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 508–567（60 aa） Fragment：sushi domain (UNP residues 508-567)	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；20% PEG3500, 0.1 M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；20% PEG3500, 0.1 M sodium acetate, soaked in 0.5 M sodium iodide, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 1.08 Å R-free 0.170
4ONT Ternary host recognition complex of complement factor H, C3d, and sialic acid Deposited 2014-01-29	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric	Chain F 1107–1231（125 aa） Fragment：Sushi 19-20 domains (UNP residues 1107-1231)	Not recorded	GOL GLYCEROL × 3	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；293 K；0.1 M Tris-HCl, pH 9.0, 8% w/v PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.15 Å R-free 0.223
4ONT Ternary host recognition complex of complement factor H, C3d, and sialic acid Deposited 2014-01-29	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Other combination Heteromer；Protein × 2 PDB declaration: dimeric	Chain E 1107–1231（125 aa） Fragment：Sushi 19-20 domains (UNP residues 1107-1231)	Not recorded	GOL GLYCEROL × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；293 K；0.1 M Tris-HCl, pH 9.0, 8% w/v PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.15 Å R-free 0.223
4ONT Ternary host recognition complex of complement factor H, C3d, and sialic acid Deposited 2014-01-29	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Other combination Heteromer；Protein × 2 PDB declaration: dimeric	Chain D 1107–1231（125 aa） Fragment：Sushi 19-20 domains (UNP residues 1107-1231)	Not recorded	GOL GLYCEROL × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；293 K；0.1 M Tris-HCl, pH 9.0, 8% w/v PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.15 Å R-free 0.223
4ZH1 Complement factor H in complex with the GM1 glycan Deposited 2015-04-24	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric	Chain E 1107–1231（125 aa） Fragment：residues 1107-1231	Not recorded	GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION；293 K；0.1 M TRIS-HCL, PH 9.0, 8% W/V PRG 8000	Resolution 2.24 Å R-free 0.228
4ZH1 Complement factor H in complex with the GM1 glycan Deposited 2015-04-24	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Other combination Heteromer；Protein × 2 PDB declaration: dimeric	Chain F 1107–1231（125 aa） Fragment：residues 1107-1231	Not recorded	GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION；293 K；0.1 M TRIS-HCL, PH 9.0, 8% W/V PRG 8000	Resolution 2.24 Å R-free 0.228
4ZH1 Complement factor H in complex with the GM1 glycan Deposited 2015-04-24	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Other combination Heteromer；Protein × 2 PDB declaration: dimeric	Chain D 1107–1231（125 aa） Fragment：residues 1107-1231	Not recorded	GOL GLYCEROL × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION；293 K；0.1 M TRIS-HCL, PH 9.0, 8% W/V PRG 8000	Resolution 2.24 Å R-free 0.228
5NBQ The structure of the tripartite complex between OspE, the C-terminal domains of factor H and C3dg Deposited 2017-03-02	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain D 1104–1230（127 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；20% PEG3350, 0.1 M HEPES pH 7.5, 0.2 M MgCl2 or 24% PEG3350, 0.1 M HEPES pH 7.5, 0.2 M MgCl2 or 16% PEG3350, Tris pH 8.5, 0.2 M ammonium acetate	Resolution 3.18 Å R-free 0.261
5NBQ The structure of the tripartite complex between OspE, the C-terminal domains of factor H and C3dg Deposited 2017-03-02	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain E 1104–1230（127 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；20% PEG3350, 0.1 M HEPES pH 7.5, 0.2 M MgCl2 or 24% PEG3350, 0.1 M HEPES pH 7.5, 0.2 M MgCl2 or 16% PEG3350, Tris pH 8.5, 0.2 M ammonium acetate	Resolution 3.18 Å R-free 0.261
5NBQ The structure of the tripartite complex between OspE, the C-terminal domains of factor H and C3dg Deposited 2017-03-02	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain F 1104–1230（127 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；20% PEG3350, 0.1 M HEPES pH 7.5, 0.2 M MgCl2 or 24% PEG3350, 0.1 M HEPES pH 7.5, 0.2 M MgCl2 or 16% PEG3350, Tris pH 8.5, 0.2 M ammonium acetate	Resolution 3.18 Å R-free 0.261
5O32 The structure of complement complex Deposited 2017-05-23	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric	Chain C 19–264（246 aa） Fragment：UNP residues 19-264,UNP residues 1107-1230,UNP residues 19-264,UNP residues 1107-1230 Chain C 1107–1230（124 aa） Fragment：UNP residues 19-264,UNP residues 1107-1230,UNP residues 19-264,UNP residues 1107-1230 Chain G 19–264（246 aa） Fragment：UNP residues 19-264,UNP residues 1107-1230,UNP residues 19-264,UNP residues 1107-1230 Chain G 1107–1230（124 aa） Fragment：UNP residues 19-264,UNP residues 1107-1230,UNP residues 19-264,UNP residues 1107-1230	Not recorded	CA CALCIUM ION × 6 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 12 MLI MALONATE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291.15 K；60mM sodium malonate PH5.0 6% w/v PEG3350	Resolution 4.21 Å R-free 0.254
5O35 Structure of complement proteins complex Deposited 2017-05-23	Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric	Chain C 19–264（246 aa） Chain C 1107–1231（125 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291.15 K；60mM Ammonium sulfate, 6% w/v PEG3350	Resolution 4.20 Å R-free 0.246
5WTB Complex Structure of Staphylococcus aureus SdrE with human complement factor H Deposited 2016-12-10	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain H 1206–1226（21 aa） Fragment：UNP residues 1206-1226	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；0.1M HEPES sodium pH 7.5, 0.2M Magnesium chloride, 30% PEG 400 (v/v)	Resolution 3.30 Å R-free 0.281
5WTB Complex Structure of Staphylococcus aureus SdrE with human complement factor H Deposited 2016-12-10	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain E 1206–1226（21 aa） Fragment：UNP residues 1206-1226	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；0.1M HEPES sodium pH 7.5, 0.2M Magnesium chloride, 30% PEG 400 (v/v)	Resolution 3.30 Å R-free 0.281
5WTB Complex Structure of Staphylococcus aureus SdrE with human complement factor H Deposited 2016-12-10	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain F 1206–1226（21 aa） Fragment：UNP residues 1206-1226	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；0.1M HEPES sodium pH 7.5, 0.2M Magnesium chloride, 30% PEG 400 (v/v)	Resolution 3.30 Å R-free 0.281
5WTB Complex Structure of Staphylococcus aureus SdrE with human complement factor H Deposited 2016-12-10	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain G 1206–1226（21 aa） Fragment：UNP residues 1206-1226	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；0.1M HEPES sodium pH 7.5, 0.2M Magnesium chloride, 30% PEG 400 (v/v)	Resolution 3.30 Å R-free 0.281
6ZH1 Crystal structure of complex between FH19-20 and FhbA protein from Borrelia hermsii Deposited 2020-06-20	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 1104–1231（128 aa）	Not recorded	EDO 1,2-ETHANEDIOL × 4 SO4 SULFATE ION × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.7；293.15 K；0.1 M MES pH6.7, 0.2 M Ammonium Sulfate, 20% (v/v) PEG 4000	Resolution 2.20 Å R-free 0.240
7WKI Structure of the ultra-affinity complex between CFH and a nanobody Deposited 2022-01-10	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 1046–1231（186 aa） Fragment：C-terminal domain	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Sodium Phosphate monohydrate, 20% PEG 3350	Resolution 2.60 Å R-free 0.239
7ZJM Crystal structure of a complex between CspZ from Borrelia burgdorferi strain B408 and human FH SCR domains 6-7 Deposited 2022-04-11	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 321–444（124 aa）	Not recorded	ZN ZINC ION × 7 IMD IMIDAZOLE × 4 PEG DI(HYDROXYETHYL)ETHER × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.4；294 K；0.2 M Zinc acetate 0.1 M imidazole (pH 7.4) 10% PEG 3000	Resolution 2.59 Å R-free 0.276
9F7I Crystal structure of Borrelia burgdorferi B31 CspZ in complex with human FH SCR6-7 Deposited 2024-05-03	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 321–444（124 aa）	Not recorded	ZN ZINC ION × 1 SO4 SULFATE ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；294 K；0.25 M ammonium sulfate 0.1 M MES pH 6.5 10% PEG 3000	Resolution 2.85 Å R-free 0.261
9MLU FbaA with Factor H 6-7 domain Deposited 2024-12-19	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 321–443（123 aa）	Not recorded	MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；MES pH 6.0, Sodium chloride, PEG 6000, Ethylene Glycol	Resolution 1.82 Å R-free 0.276
9MLU FbaA with Factor H 6-7 domain Deposited 2024-12-19	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 321–443（123 aa）	Not recorded	MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；MES pH 6.0, Sodium chloride, PEG 6000, Ethylene Glycol	Resolution 1.82 Å R-free 0.276
9MMX M6 protein with Factor H 6-7 domain Deposited 2024-12-20	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain A 321–443（123 aa）	Not recorded	EDO 1,2-ETHANEDIOL × 3	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；PEG 6000, ethylene glycol, Sodium chloride	Resolution 1.90 Å R-free 0.302
9MMX M6 protein with Factor H 6-7 domain Deposited 2024-12-20	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain B 321–443（123 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；PEG 6000, ethylene glycol, Sodium chloride	Resolution 1.90 Å R-free 0.302

47 other PDB entries and 74 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	CFAH_HUMAN
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain C； PDBConstruct 3–125； UniProt 321–443 Author chain D； PDBConstruct 3–125； UniProt 321–443 Author chain G； PDBConstruct 3–125； UniProt 321–443 Author chain H； PDBConstruct 3–125； UniProt 321–443

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

Download Download SAXS (.dat) P(r) (.pr.out) CIF

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id	9mmu
Deposition date deposition_date	2024-12-20
Structure title title	M5 protein with Factor H 6-7 domain
Keywords keywords	complement evasion, M protein, Factor H, IMMUNE SYSTEM; IMMUNE SYSTEM
Experimental Method method	X-RAY DIFFRACTION

▼

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	35.75 Å
Radius of gyration Rg (electron density) rg_electron	35.91 Å
Forward intensity I(0) i0	79589700.00
Molecular weight molecular_weight	64325.0 kDa
Excluded volume excluded_volume	77109 Å³
Envelope volume envelope_volume	122490 Å³
Hydration-shell volume shell_volume	30309 Å³
Envelope diameter envelope_diameter	136.3 Å
Shell Rg shell_rg	39.96 Å
Envelope Rg envelope_rg	34.46 Å
Shape Rg shape_rg	35.89 Å
Total Rg total_rg	36.26 Å
Total atoms total_atoms	4544
Residues n_residues	636
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

▼

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	127.4 Å
Rg (real space) rg_real	35.93 Å
Rg uncertainty (real space) rg_real_error	1.58 Å
I(0) (real space) i0_real	7.9590e+07
I(0) uncertainty (real space) i0_real_error	1.4360e+06
Rg (reciprocal space) rg_reciprocal	35.82 Å
I(0) (reciprocal space) i0_reciprocal	79580000.0000
Solution quality estimate total_estimate	0.8690
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	2
Primary peak position r_peak_primary	40.5 Å
Skewness Skewness skewness	0.401
Kurtosis Kurtosis kurtosis	-0.308
Angular range angular_range	— – 0.2200 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	3221000.0000
Real-space data points n_real_points	45
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.816; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.853; Smooth: 0.990

▶

5. Crystallography and Experiment 5. Crystallography & Experiment

▶

6. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Description description	M protein, serotype 5
Molecular weight formula_weight	9983.9 kDa
Source method src_method	man
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,E,F
Atom count atom_count	4614

GPGSNEGLKTEKKEHEAENDKLKQQRDTLSTQKETLEREVQNTQYNNETLKIKNGDLTKELNKTRQELANKQQESKENEKALNELL

来源 Source

来源生物 organism	Streptococcus pyogenes str. Manfredo
NCBI Class编号 ncbi_taxonomy_id	160491
宿主生物 host_organism	;Escherichia coli 'BL21-Gold(DE3)pLysS AG' ;

Entity 2 Entity 2 polymer

Description description	Complement factor H
Molecular weight formula_weight	14435.3 kDa
Source method src_method	man
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C,D,G,H

MGTLKPCDYPDIKHGGLYHENMRRPYFPVAVGKYYSYYCDEHFETPSGSYWDHIHCTQDGWSPAVPCLRKCYFPYLENGYNQNYGRKFVQGKSIDVACHPGYALPKAQTTVTCMENGWSPTPRCI

来源 Source

来源生物 organism	Homo sapiens
通用名 common_name	human
NCBI Class编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	;Escherichia coli 'BL21-Gold(DE3)pLysS AG' ;

Entity 3 Entity 3 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	70

▶

8. Citations (1)

▶

Unit-cell length a length_a	62.767 Å
Unit-cell length b length_b	62.767 Å
Unit-cell length c length_c	199.335 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	120.00 °
Molecules per unit cell Z z_pdb	12

Space group space_group	P 32
Space-group number int_tables_number	145
Crystal system crystal_system	trigonal

Resolution resolution	2.250 Å
Low-resolution limit resolution_low	47.720 Å
R factor r_factor	0.2685
R_work r_work	0.2534
R_free r_free	0.2872

fit	/app/data/saxs_computation/mm/9mmu/9mmu.fit
pddf	/app/data/pr_computation/mm/9mmu/9mmu.pr.out
profile	/app/data/saxs_computation/mm/9mmu/9mmu.dat

M5 protein with Factor H 6-7 domain

1. Protein Identity and Related Structures Protein Identity & Related Structures

M protein, serotype 5

State in the Current Structure

Other States of the Same Protein in the Database

Complement factor H

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

6. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

来源 Source

Entity 2 Entity 2 polymer

Polymer Polymer

来源 Source

Entity 3 Entity 3 water

8. Citations (1)

9. Files and Curves (10)

Files (7)

Curves (3)