9ngu

In situ cryo-EM structure of outer membrane cap (OMC) of the Legionella Dot/Icm T4SS machine

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

DotD

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 130	LphA (DotK) × 13 （Q5ZYC5） Putative auto-transporter adhesin head GIN domain-containing protein × 13 （Q5ZRN3） Neurogenic locus notch protein homolog × 13 （Q5ZXA9） IcmG (DotF) × 13 （Q5ZYC0） IcmK (DotH) × 13 （Q5ZYC2） Outer membrane protein, OmpA family protein × 13 （Q5ZXS4） DotC × 13 （Q5ZS44） Secreted protein × 13 （A0AAN5PM39）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	Q5ZS45_LEGPH
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain Aa； PDBConstruct 1–163； UniProt 1–163 Author chain Ae； PDBConstruct 1–163； UniProt 1–163 Author chain Ak； PDBConstruct 1–163； UniProt 1–163 Author chain Ao； PDBConstruct 1–163； UniProt 1–163 Author chain Au； PDBConstruct 1–163； UniProt 1–163 Author chain Ay； PDBConstruct 1–163； UniProt 1–163 Author chain Be； PDBConstruct 1–163； UniProt 1–163 Author chain Bi； PDBConstruct 1–163； UniProt 1–163 Author chain Bo； PDBConstruct 1–163； UniProt 1–163 Author chain Bs； PDBConstruct 1–163； UniProt 1–163 Author chain By； PDBConstruct 1–163； UniProt 1–163 Author chain Cc； PDBConstruct 1–163； UniProt 1–163 Author chain Ci； PDBConstruct 1–163； UniProt 1–163 Author chain Cm； PDBConstruct 1–163； UniProt 1–163 Author chain Cs； PDBConstruct 1–163； UniProt 1–163 Author chain Cw； PDBConstruct 1–163； UniProt 1–163 Author chain Dc； PDBConstruct 1–163； UniProt 1–163 Author chain Dg； PDBConstruct 1–163； UniProt 1–163 Author chain Dm； PDBConstruct 1–163； UniProt 1–163 Author chain Dq； PDBConstruct 1–163； UniProt 1–163 Author chain Dw； PDBConstruct 1–163； UniProt 1–163 Author chain Ea； PDBConstruct 1–163； UniProt 1–163 Author chain Eg； PDBConstruct 1–163； UniProt 1–163 Author chain Ek； PDBConstruct 1–163； UniProt 1–163 Author chain Eq； PDBConstruct 1–163； UniProt 1–163 Author chain Eu； PDBConstruct 1–163； UniProt 1–163

LphA (DotK)

OrganismNot specified

UniProt Q5ZYC5

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 130	DotD × 26 （Q5ZS45） Putative auto-transporter adhesin head GIN domain-containing protein × 13 （Q5ZRN3） Neurogenic locus notch protein homolog × 13 （Q5ZXA9） IcmG (DotF) × 13 （Q5ZYC0） IcmK (DotH) × 13 （Q5ZYC2） Outer membrane protein, OmpA family protein × 13 （Q5ZXS4） DotC × 13 （Q5ZS44） Secreted protein × 13 （A0AAN5PM39）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	Q5ZYC5_LEGPH
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain Ab； PDBConstruct 1–189； UniProt 1–189 Author chain Al； PDBConstruct 1–189； UniProt 1–189 Author chain Av； PDBConstruct 1–189； UniProt 1–189 Author chain Bf； PDBConstruct 1–189； UniProt 1–189 Author chain Bp； PDBConstruct 1–189； UniProt 1–189 Author chain Bz； PDBConstruct 1–189； UniProt 1–189 Author chain Cj； PDBConstruct 1–189； UniProt 1–189 Author chain Ct； PDBConstruct 1–189； UniProt 1–189 Author chain Dd； PDBConstruct 1–189； UniProt 1–189 Author chain Dn； PDBConstruct 1–189； UniProt 1–189 Author chain Dx； PDBConstruct 1–189； UniProt 1–189 Author chain Eh； PDBConstruct 1–189； UniProt 1–189 Author chain Er； PDBConstruct 1–189； UniProt 1–189

Putative auto-transporter adhesin head GIN domain-containing protein

OrganismNot specified

UniProt Q5ZRN3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 130	DotD × 26 （Q5ZS45） LphA (DotK) × 13 （Q5ZYC5） Neurogenic locus notch protein homolog × 13 （Q5ZXA9） IcmG (DotF) × 13 （Q5ZYC0） IcmK (DotH) × 13 （Q5ZYC2） Outer membrane protein, OmpA family protein × 13 （Q5ZXS4） DotC × 13 （Q5ZS44） Secreted protein × 13 （A0AAN5PM39）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	Q5ZRN3_LEGPH
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain Ac； PDBConstruct 1–320； UniProt 1–320 Author chain Am； PDBConstruct 1–320； UniProt 1–320 Author chain Aw； PDBConstruct 1–320； UniProt 1–320 Author chain Bg； PDBConstruct 1–320； UniProt 1–320 Author chain Bq； PDBConstruct 1–320； UniProt 1–320 Author chain Ca； PDBConstruct 1–320； UniProt 1–320 Author chain Ck； PDBConstruct 1–320； UniProt 1–320 Author chain Cu； PDBConstruct 1–320； UniProt 1–320 Author chain De； PDBConstruct 1–320； UniProt 1–320 Author chain Do； PDBConstruct 1–320； UniProt 1–320 Author chain Dy； PDBConstruct 1–320； UniProt 1–320 Author chain Ei； PDBConstruct 1–320； UniProt 1–320 Author chain Es； PDBConstruct 1–320； UniProt 1–320

Neurogenic locus notch protein homolog

OrganismNot specified

UniProt Q5ZXA9

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 130	DotD × 26 （Q5ZS45） LphA (DotK) × 13 （Q5ZYC5） Putative auto-transporter adhesin head GIN domain-containing protein × 13 （Q5ZRN3） IcmG (DotF) × 13 （Q5ZYC0） IcmK (DotH) × 13 （Q5ZYC2） Outer membrane protein, OmpA family protein × 13 （Q5ZXS4） DotC × 13 （Q5ZS44） Secreted protein × 13 （A0AAN5PM39）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	Q5ZXA9_LEGPH
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain Ad； PDBConstruct 1–124； UniProt 1–124 Author chain An； PDBConstruct 1–124； UniProt 1–124 Author chain Ax； PDBConstruct 1–124； UniProt 1–124 Author chain Bh； PDBConstruct 1–124； UniProt 1–124 Author chain Br； PDBConstruct 1–124； UniProt 1–124 Author chain Cb； PDBConstruct 1–124； UniProt 1–124 Author chain Cl； PDBConstruct 1–124； UniProt 1–124 Author chain Cv； PDBConstruct 1–124； UniProt 1–124 Author chain Df； PDBConstruct 1–124； UniProt 1–124 Author chain Dp； PDBConstruct 1–124； UniProt 1–124 Author chain Dz； PDBConstruct 1–124； UniProt 1–124 Author chain Ej； PDBConstruct 1–124； UniProt 1–124 Author chain Et； PDBConstruct 1–124； UniProt 1–124

IcmG (DotF)

OrganismNot specified

UniProt Q5ZYC0

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 130	DotD × 26 （Q5ZS45） LphA (DotK) × 13 （Q5ZYC5） Putative auto-transporter adhesin head GIN domain-containing protein × 13 （Q5ZRN3） Neurogenic locus notch protein homolog × 13 （Q5ZXA9） IcmK (DotH) × 13 （Q5ZYC2） Outer membrane protein, OmpA family protein × 13 （Q5ZXS4） DotC × 13 （Q5ZS44） Secreted protein × 13 （A0AAN5PM39）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	Q5ZYC0_LEGPH
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain Af； PDBConstruct 1–269； UniProt 1–269 Author chain Ap； PDBConstruct 1–269； UniProt 1–269 Author chain Az； PDBConstruct 1–269； UniProt 1–269 Author chain Bj； PDBConstruct 1–269； UniProt 1–269 Author chain Bt； PDBConstruct 1–269； UniProt 1–269 Author chain Cd； PDBConstruct 1–269； UniProt 1–269 Author chain Cn； PDBConstruct 1–269； UniProt 1–269 Author chain Cx； PDBConstruct 1–269； UniProt 1–269 Author chain Dh； PDBConstruct 1–269； UniProt 1–269 Author chain Dr； PDBConstruct 1–269； UniProt 1–269 Author chain Eb； PDBConstruct 1–269； UniProt 1–269 Author chain El； PDBConstruct 1–269； UniProt 1–269 Author chain Ev； PDBConstruct 1–269； UniProt 1–269

IcmK (DotH)

OrganismNot specified

UniProt Q5ZYC2

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 130	DotD × 26 （Q5ZS45） LphA (DotK) × 13 （Q5ZYC5） Putative auto-transporter adhesin head GIN domain-containing protein × 13 （Q5ZRN3） Neurogenic locus notch protein homolog × 13 （Q5ZXA9） IcmG (DotF) × 13 （Q5ZYC0） Outer membrane protein, OmpA family protein × 13 （Q5ZXS4） DotC × 13 （Q5ZS44） Secreted protein × 13 （A0AAN5PM39）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	Q5ZYC2_LEGPH
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain Ag； PDBConstruct 1–361； UniProt 1–361 Author chain Aq； PDBConstruct 1–361； UniProt 1–361 Author chain Ba； PDBConstruct 1–361； UniProt 1–361 Author chain Bk； PDBConstruct 1–361； UniProt 1–361 Author chain Bu； PDBConstruct 1–361； UniProt 1–361 Author chain Ce； PDBConstruct 1–361； UniProt 1–361 Author chain Co； PDBConstruct 1–361； UniProt 1–361 Author chain Cy； PDBConstruct 1–361； UniProt 1–361 Author chain Di； PDBConstruct 1–361； UniProt 1–361 Author chain Ds； PDBConstruct 1–361； UniProt 1–361 Author chain Ec； PDBConstruct 1–361； UniProt 1–361 Author chain Em； PDBConstruct 1–361； UniProt 1–361 Author chain Ew； PDBConstruct 1–361； UniProt 1–361

Outer membrane protein, OmpA family protein

OrganismNot specified

UniProt Q5ZXS4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 130	DotD × 26 （Q5ZS45） LphA (DotK) × 13 （Q5ZYC5） Putative auto-transporter adhesin head GIN domain-containing protein × 13 （Q5ZRN3） Neurogenic locus notch protein homolog × 13 （Q5ZXA9） IcmG (DotF) × 13 （Q5ZYC0） IcmK (DotH) × 13 （Q5ZYC2） DotC × 13 （Q5ZS44） Secreted protein × 13 （A0AAN5PM39）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	Q5ZXS4_LEGPH
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain Ah； PDBConstruct 1–249； UniProt 1–249 Author chain Ar； PDBConstruct 1–249； UniProt 1–249 Author chain Bb； PDBConstruct 1–249； UniProt 1–249 Author chain Bl； PDBConstruct 1–249； UniProt 1–249 Author chain Bv； PDBConstruct 1–249； UniProt 1–249 Author chain Cf； PDBConstruct 1–249； UniProt 1–249 Author chain Cp； PDBConstruct 1–249； UniProt 1–249 Author chain Cz； PDBConstruct 1–249； UniProt 1–249 Author chain Dj； PDBConstruct 1–249； UniProt 1–249 Author chain Dt； PDBConstruct 1–249； UniProt 1–249 Author chain Ed； PDBConstruct 1–249； UniProt 1–249 Author chain En； PDBConstruct 1–249； UniProt 1–249 Author chain Ex； PDBConstruct 1–249； UniProt 1–249

DotC

OrganismNot specified

UniProt Q5ZS44

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 130	DotD × 26 （Q5ZS45） LphA (DotK) × 13 （Q5ZYC5） Putative auto-transporter adhesin head GIN domain-containing protein × 13 （Q5ZRN3） Neurogenic locus notch protein homolog × 13 （Q5ZXA9） IcmG (DotF) × 13 （Q5ZYC0） IcmK (DotH) × 13 （Q5ZYC2） Outer membrane protein, OmpA family protein × 13 （Q5ZXS4） Secreted protein × 13 （A0AAN5PM39）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	Q5ZS44_LEGPH
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain Ai； PDBConstruct 1–303； UniProt 1–303 Author chain As； PDBConstruct 1–303； UniProt 1–303 Author chain Bc； PDBConstruct 1–303； UniProt 1–303 Author chain Bm； PDBConstruct 1–303； UniProt 1–303 Author chain Bw； PDBConstruct 1–303； UniProt 1–303 Author chain Cg； PDBConstruct 1–303； UniProt 1–303 Author chain Cq； PDBConstruct 1–303； UniProt 1–303 Author chain Da； PDBConstruct 1–303； UniProt 1–303 Author chain Dk； PDBConstruct 1–303； UniProt 1–303 Author chain Du； PDBConstruct 1–303； UniProt 1–303 Author chain Ee； PDBConstruct 1–303； UniProt 1–303 Author chain Eo； PDBConstruct 1–303； UniProt 1–303 Author chain Ey； PDBConstruct 1–303； UniProt 1–303

Secreted protein

OrganismNot specified

UniProt A0AAN5PM39

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 130	DotD × 26 （Q5ZS45） LphA (DotK) × 13 （Q5ZYC5） Putative auto-transporter adhesin head GIN domain-containing protein × 13 （Q5ZRN3） Neurogenic locus notch protein homolog × 13 （Q5ZXA9） IcmG (DotF) × 13 （Q5ZYC0） IcmK (DotH) × 13 （Q5ZYC2） Outer membrane protein, OmpA family protein × 13 （Q5ZXS4） DotC × 13 （Q5ZS44）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0AAN5PM39_LEGPN
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain Aj； PDBConstruct 1–93； UniProt 1–93 Author chain At； PDBConstruct 1–93； UniProt 1–93 Author chain Bd； PDBConstruct 1–93； UniProt 1–93 Author chain Bn； PDBConstruct 1–93； UniProt 1–93 Author chain Bx； PDBConstruct 1–93； UniProt 1–93 Author chain Ch； PDBConstruct 1–93； UniProt 1–93 Author chain Cr； PDBConstruct 1–93； UniProt 1–93 Author chain Db； PDBConstruct 1–93； UniProt 1–93 Author chain Dl； PDBConstruct 1–93； UniProt 1–93 Author chain Dv； PDBConstruct 1–93； UniProt 1–93 Author chain Ef； PDBConstruct 1–93； UniProt 1–93 Author chain Ep； PDBConstruct 1–93； UniProt 1–93 Author chain Ez； PDBConstruct 1–93； UniProt 1–93

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	9ngu
Deposition date deposition_date	2025-02-22
Structure title title	In situ cryo-EM structure of outer membrane cap (OMC) of the Legionella Dot/Icm T4SS machine
Keywords keywords	Type IVB Dot/Icm Secretion Machine, PROTEIN TRANSPORT; PROTEIN TRANSPORT
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

9ngu__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

9ngu__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

9ngu__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	0.00 Å
Rg (electron density)	133.80 Å
Total Rg	133.70 Å
Atom count	147498
Residues	18915
Excluded volume	2635300 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`9ngu__assembly_1__model_1`	130-meric （130）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (9)

Entity 1 Entity 1 polymer

Description description	DotD
Molecular weight formula_weight	17860.7 kDa
Source method src_method	nat
Copy count entity_count	26

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Aa,Ae,Ak,Ao,Au,Ay,Be,Bi,Bo,Bs,By,Cc,Ci,Cm,Cs,Cw,Dc,Dg,Dm,Dq,Dw,Ea,Eg,Ek,Eq,Eu
Atom count atom_count	147498

MNNNKIVIMFIFSALLAGCAGTMKFKKPPINNPSDDATIKLAEAAVSVSDSMLEMAKVEKVITPPSKDNTLTIPNAYNLQARASVDWSGPIEELTARIAKAAHFRFRVLGKSPSVPVLISISTKDESLAEILRDIDYQAGKKASIHVYPNSQVVELRYAKIYS

Entity 2 Entity 2 polymer

Description description	LphA (DotK)
Molecular weight formula_weight	21096.5 kDa
Source method src_method	nat
Copy count entity_count	13

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Ab,Al,Av,Bf,Bp,Bz,Cj,Ct,Dd,Dn,Dx,Eh,Er

MRSLRTNYIYVLFKTTGLLFLLLLSACNRSGYIPENEVPKLPCRVDGACDATIIKMMTDLNKKGIKVASVGQNYLISIPASALFADQSPRLNWASYSLLNEIAAFLKQFRKIAITVTSYSSKYVSVKRERALTLARSRVVSEYLWSQGVDSRIIFTQGLGSDKPITSYTLGGDRSPNARVEITFRRAVA

Entity 3 Entity 3 polymer

Description description	Putative auto-transporter adhesin head GIN domain-containing protein
Molecular weight formula_weight	36095.4 kDa
Source method src_method	nat
Copy count entity_count	13

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Ac,Am,Aw,Bg,Bq,Ca,Ck,Cu,De,Do,Dy,Ei,Es

MLKRCYLLILLMFVLASCAHHKPQTPPAEVKKQGTSSTRQFRQVSSFNQIVVQGRLNVNLHTGYNKPEVMLRGDPRDLVQVRTIVKQNTLYVSLGQGYPDYGAVTVDIKTKFLNRFRYEGAGVVTGNNLRTSYLDLYLANEGTTRLAGNIGLQKLEAVGNGVTQINGVSSRNLQIVLKGDPKVLISGFVNLRQLDMYGKGTLSLYWIKSDTLTIRAKKAAKIQLAGIVNRLDVELWDFAQFKGKYLRAQRSFVKTHDKSVAEISAVNHQSSLATDASDIYYYNLSKTRADFMAFNGSVLDMREWGQSDLKDFDRYNKQFP

Entity 4 Entity 4 polymer

Description description	Neurogenic locus notch protein homolog
Molecular weight formula_weight	13598.9 kDa
Source method src_method	nat
Copy count entity_count	13

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Ad,An,Ax,Bh,Br,Cb,Cl,Cv,Df,Dp,Dz,Ej,Et

MLFLKIKTNQRTTMNILKPKAFLLASVFVLSISPAFAADGCCSKMGGINYCDSSAGRLVCNNGFYSTCYCTRHAVMDLQFLMGCCLWHGGVYPQLNSSGLVVCNDGYVSEECSLQKPVEQISVY

Entity 5 Entity 5 polymer

Description description	IcmG (DotF)
Molecular weight formula_weight	29730.0 kDa
Source method src_method	nat
Copy count entity_count	13

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Af,Ap,Az,Bj,Bt,Cd,Cn,Cx,Dh,Dr,Eb,El,Ev

MMAEHDQNNDEYKFAELDSYDMDQAGESDLDSEASYQSGKEGLTKKKDIKRNALIAIGAVVFIMVMYKIIGWMFFSDKSSQVTSKPAIPPVTQVATPQPVQTIPTTTPIQQVQPTTIIEDDPDLKKKVSAIEMTQQSLRSEVNALSEQINAVNNNIKNLNAQIVNLNQIIGNMSNQIARQSEVINVLMARTTPKKVVKVSRPIVQARIIYYIQAVIPGRAWLIGSNGSTLTVREGSKIPGYGMVKLIDSLQGRILTSSGQVIKFSQEDS

Entity 6 Entity 6 polymer

Description description	IcmK (DotH)
Molecular weight formula_weight	38958.9 kDa
Source method src_method	nat
Copy count entity_count	13

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Ag,Aq,Ba,Bk,Bu,Ce,Co,Cy,Di,Ds,Ec,Em,Ew

MMKKYDQLCKYCLVIGLTFSMSCSIYAADQSDDAQQALQQLRMLQQKLSQNPSPDAQSGAGDGGDNAASDSTQQPNQSGQANAPAANQTATAGGDGQIISQDDAEVIDKKAFKDMTRNLYPLNPEQVVKLKQIYETSEYAKAATPGTPPKPTATSQFVNLSPGSTPPVIRLSQGFVSSLVFLDSTGAPWPIAAYDLGDPSSFNIQWDKTSNTLMIQATKLYNYGNLAVRLRGLNTPVMLTLIPGQKAVDYRVDLRVQGYGPNAKSMPTEEGIPPSANDLLLHVLEGVPPPGSRRLVVSGGDARAWLSNEKMYVRTNLTILSPGWLASMTSADGTHAYEMQKSPVLLVSWHGKVMQLKVEGL

Entity 7 Entity 7 polymer

Description description	Outer membrane protein, OmpA family protein
Molecular weight formula_weight	27582.0 kDa
Source method src_method	nat
Copy count entity_count	13

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Ah,Ar,Bb,Bl,Bv,Cf,Cp,Cz,Dj,Dt,Ed,En,Ex

MRNLMRCLIMIKSLIKGVDMSRKLAKTRILGYGLMICFLAGCFHPPYNNFQPDRRAVKRVGVDTGIGAVAGAIASGTASGTLIGAAAGGTVGLVASIYRDSKRKIIRDLQKQDIQYVEYGDTRTLIIPTDKYFMFSSPRLNEICYPGLNNVIRLLNFYPQSTIYVAGFTDNVGSRSHKRKLSQAQAETMMTFLWANGIAAKRLKAEGYGDKNAISDNAIIHGSAQNRRIEIQWFTSPAQPPQPQMAYVK

Entity 8 Entity 8 polymer

Description description	DotC
Molecular weight formula_weight	34162.4 kDa
Source method src_method	nat
Copy count entity_count	13

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Ai,As,Bc,Bm,Bw,Cg,Cq,Da,Dk,Du,Ee,Eo,Ey

MRKFILSLSILLSALLVACSSRNHYGDTGSLAGLQAMADSKYTRAQKKQKMGKIREMALKETALSVGAQAGLAWRAKIIDEQLNKQARNLDAIYDFNSLVLEHNILPPVLLEGRNTLNLADAQSIRISDRTYKVAKQAHFITTPPTWRQYLWMDYVKPEAPNVTLLPKTKAEKEIWCIYTERGWKNGIDQANTILEENIARIKEDFGGMILYRKLLAMNMVSPPYVSHTDLGVTGDGSEIHIDDRVLRITALPELNVNSAEWRAAVAKDENALERFKNMEKLANQAKIVITNKSWQPIIAPVS

Entity 9 Entity 9 polymer

Description description	Secreted protein
Molecular weight formula_weight	10348.0 kDa
Source method src_method	nat
Copy count entity_count	13

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Aj,At,Bd,Bn,Bx,Ch,Cr,Db,Dl,Dv,Ef,Ep,Ez

MKNKLAVSILLFLLPTAAAMANCDLTKFRWDCDLPVQVKPSPASSLLVYCGSSYGYITKQQYDILARYQRASVNMVLDINGEYIDSPCIGAER

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	triclinic

Resolution resolution	2.96 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	76409

In situ cryo-EM structure of outer membrane cap (OMC) of the Legionella Dot/Icm T4SS machine

1. Protein Identity and Related Structures Protein Identity & Related Structures

DotD

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

LphA (DotK)

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Putative auto-transporter adhesin head GIN domain-containing protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Neurogenic locus notch protein homolog

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

IcmG (DotF)

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

IcmK (DotH)

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Outer membrane protein, OmpA family protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DotC

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Secreted protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

9ngu__assembly_1__model_1

9ngu__assembly_1__model_1 | I(q)

9ngu__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment