9r4a
Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: Human insulin, pH 4.5 (50 ms soaking)
SAXS Profile
P(r) Distribution
1. Structure Basics
| entry_id | 9r4a |
| deposition_date | 2025-05-07 |
| last_revision | 2025-12-03 |
| title | Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: Human insulin, pH 4.5 (50 ms soaking) |
| keywords | Human insulin, pH-jump, pH 4.5, Spitrobot-2, HORMONE; HORMONE |
| method | X-RAY DIFFRACTION |
Download Data
2. SAXS Parameters (CRYSOL)
| rg_guinier | 11.34 Å |
| rg_electron | 9.95 Å |
| i0 | 887083.00 |
| molecular_weight | 5908.0 kDa |
| excluded_volume | 7266 ų |
| envelope_volume | 7799 ų |
| shell_volume | 6996 ų |
| envelope_diameter | 34.0 Å |
| shell_rg | 15.06 Å |
| envelope_rg | 10.27 Å |
| shape_rg | 9.96 Å |
| total_rg | 11.38 Å |
| total_atoms | 410 |
| n_residues | 51 |
| n_harmonics | 20 |
| q_range | — – 0.5000 Å−1 |
| n_points | 101 |
| shell_type | directional |
| solvent_density | 0.3340 e/ų |
| contrast_shell | 0.0300 e/ų |
| crysol_version | 4.1.3 |
3. P(r) Analysis (GNOM)
| dmax | 37.0 Å |
| rg_real | 11.27 Å |
| rg_real_error | 0.31 Å |
| i0_real | 8.8710e+05 |
| i0_real_error | 8.2530e+03 |
| rg_reciprocal | 11.28 Å |
| i0_reciprocal | 887100.0000 |
| total_estimate | 0.8837 |
| solution_quality | GOOD a GOOD solution |
| n_peaks | 2 |
| r_peak_primary | 15.0 Å |
| skewness | 0.107 |
| kurtosis | -0.319 |
| angular_range | — – 0.5000 Å−1 |
| current_alpha | 0.0000 |
| highest_alpha | 83830.0000 |
| n_real_points | 80 |
| gnom_version | 4.1.3 |
| quality_criteria | AN1: 0.000; Oscil: 0.836; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.976 |