9r4c
Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: Human insulin, pH 4.5 (500 ms soaking)
SAXS Profile
P(r) Distribution
1. Structure Basics
| entry_id | 9r4c |
| deposition_date | 2025-05-07 |
| last_revision | 2025-12-03 |
| title | Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: Human insulin, pH 4.5 (500 ms soaking) |
| keywords | Human insulin, pH-jump, HORMONE; HORMONE |
| method | X-RAY DIFFRACTION |
Download Data
2. SAXS Parameters (CRYSOL)
| rg_guinier | 11.45 Å |
| rg_electron | 9.99 Å |
| i0 | 891407.00 |
| molecular_weight | 5908.0 kDa |
| excluded_volume | 7266 ų |
| envelope_volume | 7855 ų |
| shell_volume | 7014 ų |
| envelope_diameter | 35.4 Å |
| shell_rg | 15.15 Å |
| envelope_rg | 10.36 Å |
| shape_rg | 9.99 Å |
| total_rg | 11.44 Å |
| total_atoms | 410 |
| n_residues | 51 |
| n_harmonics | 20 |
| q_range | — – 0.5000 Å−1 |
| n_points | 101 |
| shell_type | directional |
| solvent_density | 0.3340 e/ų |
| contrast_shell | 0.0300 e/ų |
| crysol_version | 4.1.3 |
3. P(r) Analysis (GNOM)
| dmax | 38.0 Å |
| rg_real | 11.39 Å |
| rg_real_error | 0.26 Å |
| i0_real | 8.9140e+05 |
| i0_real_error | 9.8760e+03 |
| rg_reciprocal | 11.39 Å |
| i0_reciprocal | 891400.0000 |
| total_estimate | 0.7051 |
| solution_quality | REASONABLE a REASONABLE solution |
| n_peaks | 2 |
| r_peak_primary | 14.9 Å |
| skewness | 0.152 |
| kurtosis | -0.246 |
| angular_range | — – 0.5000 Å−1 |
| current_alpha | 0.0000 |
| highest_alpha | 91240.0000 |
| n_real_points | 80 |
| gnom_version | 4.1.3 |
| quality_criteria | AN1: 0.000; Oscil: 0.808; Stabil: 1.000; Sysdev: 0.256; Positv: 1.000; Valcen: 1.000; Smooth: 0.970 |