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9spr

p53 cancer mutant R282W in complex with DARPin C10

Method: X-RAY DIFFRACTION D_max: 74.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9spr
deposition_date	2025-09-17
last_revision	2026-04-22
title	p53 cancer mutant R282W in complex with DARPin C10
keywords	;p53, tumor suppressor, cancer mutation, protein stability, protein unfolding disease, designed ankyrin repeat proteins, thermostabilization, mutant p53 reactivation, cancer therapy, TRANSCRIPTION ;; TRANSCRIPTION
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	21.68 Å
rg_electron	20.69 Å
i0	23269600.00
molecular_weight	35262.0 kDa
excluded_volume	43520 Å³
envelope_volume	51989 Å³
shell_volume	21201 Å³
envelope_diameter	73.9 Å
shell_rg	26.99 Å
envelope_rg	20.90 Å
shape_rg	20.65 Å
total_rg	21.61 Å
total_atoms	2468
n_residues	321
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	74.5 Å
rg_real	21.67 Å
rg_real_error	0.52 Å
i0_real	2.3270e+07
i0_real_error	3.2050e+05
rg_reciprocal	21.67 Å
i0_reciprocal	23270000.0000
total_estimate	0.8723
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	24.4 Å
skewness	0.348
kurtosis	-0.326
angular_range	— – 0.3650 Å⁻¹
current_alpha	0.0000
highest_alpha	5806000.0000
n_real_points	68
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.801; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.945; Smooth: 0.989

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	Cellular tumor antigen p53
formula_weight	22750.8 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	2787

SSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDWRTEEENLRKKGE

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 2 polymer

description	DARPin C10
formula_weight	13527.3 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	E

GSDLGKKLLEAAWHGQDDEVRILMANGADVNATDQSGMTPLHLAAWRGHLEIVEVLLKTGADVNAIDRWGKTPLHLAARIGHLEIVEVLLKAGADVNAQDKFGKTPFDLAIDNGNEDIAEVLQKAA

Source

organism	synthetic construct
ncbi_taxonomy_id	32630
host_organism	Escherichia coli

Entity 3 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	1

Entity 4 non-polymer

description	1,2-ETHANEDIOL
formula_weight	62.1 kDa
src_method	syn
entity_count	6

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	265

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6. Citations (1)

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length_a	37.587 Å
length_b	93.837 Å
length_c	53.263 Å
angle_alpha	90.00 °
angle_beta	109.94 °
angle_gamma	90.00 °
z_pdb	2

space_group	P 1 21 1
int_tables_number	4
crystal_system	monoclinic

resolution	1.660 Å
resolution_low	46.900 Å
r_factor	0.1532
r_work	0.1507
r_free	0.2007

fit	/app/data/saxs_computation/sp/9spr/9spr.fit
pddf	/app/data/pr_computation/sp/9spr/9spr.pr.out
profile	/app/data/saxs_computation/sp/9spr/9spr.dat

p53 cancer mutant R282W in complex with DARPin C10

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

Entity 4 non-polymer

Entity 5 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)