9tgg

Cryo-EM structure of Spinacia oleracea cytochrome b6f complex with bound plastocyanin

Method: ELECTRON MICROSCOPY D_max: 147.2 Å Quality: REASONABLE

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

Cytochrome b6

物种未注明

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白异源复合物异源复合物蛋白 × 17 PDB 声明：17-meric（17）与蛋白拷贝数一致	链 A； UniProt 1–215 链 I； UniProt 1–215	未记录	Cytochrome b6-f complex subunit 4 × 2 （P00166） Cytochrome f × 2 （P16013） Cytochrome b6-f complex iron-sulfur subunit, chloroplastic × 2 （P08980） Cytochrome b6-f complex subunit 6 × 2 （Q9M3L0） Cytochrome b6-f complex subunit 7 × 2 （P80883） Cytochrome b6-f complex subunit 5 × 2 （P69461） Cytochrome b6-f complex subunit 8 × 2 （P61045） Plastocyanin, chloroplastic × 1 （P00289） HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 7 CLA CHLOROPHYLL A × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 BCR BETA-CAROTENE × 2 CU COPPER (II) ION × 1	ELECTRON MICROSCOPY cryo-EM缓冲液：pH 7.5 cryo-EM玻璃化条件：冷冻剂 ETHANE	分辨率 3.18 Å

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly， “相对当前条目”直接指出证据层面的不同；没有差异标签表示当前已读取字段一致。

其他 PDB	相对当前条目 9TGG	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
6RQF 3.6 Angstrom cryo-EM structure of the dimeric cytochrome b6f complex from Spinacia oleracea with natively bound thylakoid lipids and plastoquinone molecules 提交 2019-05-15	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 16 PDB 声明：hexadecameric	链 A 1–215（215 aa）链 I 1–215（215 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 BCR BETA-CAROTENE × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 6PL (4S,7R)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE × 3 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 PGV (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.58 Å
7QRM Cryo-EM structure of catalytically active Spinacia oleracea cytochrome b6f in complex with endogenous plastoquinones at 2.7 A resolution 提交 2022-01-11	聚集状态不同配体/离子不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 16 PDB 声明：hexadecameric	链 A 1–215（215 aa）链 I 1–215（215 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 7 UMQ UNDECYL-MALTOSIDE × 10 PGT (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.5 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.70 Å
7ZYV Cryo-EM structure of catalytically active Spinacia oleracea cytochrome b6f in complex with endogenous plastoquinones at 2.13 A resolution 提交 2022-05-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：octadecameric	链 A 1–215（215 aa）链 I 1–215（215 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 UMQ UNDECYL-MALTOSIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8；Buffer and the diluted protein sample were filtered through a 0.22 um syringe filter. Prior to freezing, protein sample was concentrated on centrifugal filter units with 50K MWCO. cryo-EM玻璃化条件冷冻剂 ETHANE；blot time 2 s, wait time 0 s, blot force -1	分辨率 2.13 Å
9ES7 Cryo-EM structure of Spinacia oleracea cytochrome b6f complex with water molecules at 1.94 A resolution 提交 2024-03-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：18-meric	链 A 1–215（215 aa）链 I 1–215（215 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 CLA CHLOROPHYLL A × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 1.94 Å
9ES8 Cryo-EM structure of Spinacia oleracea cytochrome b6f with decylplastoquinone bound at plastoquionol reduction site 提交 2024-03-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：octadecameric	链 A 1–215（215 aa）链 I 1–215（215 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 A1H65 Decylplastoquinone × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 CLA CHLOROPHYLL A × 2 UMQ UNDECYL-MALTOSIDE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7；final concentration of plastocyanin was 0.34 mM cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.24 Å
9ES9 Cryo-EM structure of Spinacia oleracea cytochrome b6f complex with inhibitor DBMIB bound at plastoquinol oxidation site 提交 2024-03-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：18-meric	链 A 1–215（215 aa）链 I 1–215（215 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 BNT 2,5-DIBROMO-3-ISOPROPYL-6-METHYLBENZO-1,4-QUINONE × 2 CLA CHLOROPHYLL A × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.33 Å

共 6 个其他 PDB 条目、6 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	CYB6_SPIOL
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 1–215； UniProt 1–215 作者链 I； PDB构建体 1–215； UniProt 1–215

Cytochrome b6-f complex subunit 4

物种未注明

UniProt P00166

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白异源复合物异源复合物蛋白 × 17 PDB 声明：17-meric（17）与蛋白拷贝数一致	链 B； UniProt 1–160 链 J； UniProt 1–160	未记录	Cytochrome b6 × 2 （P00165） Cytochrome f × 2 （P16013） Cytochrome b6-f complex iron-sulfur subunit, chloroplastic × 2 （P08980） Cytochrome b6-f complex subunit 6 × 2 （Q9M3L0） Cytochrome b6-f complex subunit 7 × 2 （P80883） Cytochrome b6-f complex subunit 5 × 2 （P69461） Cytochrome b6-f complex subunit 8 × 2 （P61045） Plastocyanin, chloroplastic × 1 （P00289） HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 7 CLA CHLOROPHYLL A × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 BCR BETA-CAROTENE × 2 CU COPPER (II) ION × 1	ELECTRON MICROSCOPY cryo-EM缓冲液：pH 7.5 cryo-EM玻璃化条件：冷冻剂 ETHANE	分辨率 3.18 Å

数据库中的同蛋白其他状态

其他 PDB	相对当前条目 9TGG	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
6RQF 3.6 Angstrom cryo-EM structure of the dimeric cytochrome b6f complex from Spinacia oleracea with natively bound thylakoid lipids and plastoquinone molecules 提交 2019-05-15	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 16 PDB 声明：hexadecameric	链 B 1–160（160 aa）链 J 1–160（160 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 BCR BETA-CAROTENE × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 6PL (4S,7R)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE × 3 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 PGV (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.58 Å
7QRM Cryo-EM structure of catalytically active Spinacia oleracea cytochrome b6f in complex with endogenous plastoquinones at 2.7 A resolution 提交 2022-01-11	聚集状态不同配体/离子不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 16 PDB 声明：hexadecameric	链 B 1–160（160 aa）链 J 1–160（160 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 7 UMQ UNDECYL-MALTOSIDE × 10 PGT (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.5 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.70 Å
7ZYV Cryo-EM structure of catalytically active Spinacia oleracea cytochrome b6f in complex with endogenous plastoquinones at 2.13 A resolution 提交 2022-05-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：octadecameric	链 B 1–160（160 aa）链 J 1–160（160 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 UMQ UNDECYL-MALTOSIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8；Buffer and the diluted protein sample were filtered through a 0.22 um syringe filter. Prior to freezing, protein sample was concentrated on centrifugal filter units with 50K MWCO. cryo-EM玻璃化条件冷冻剂 ETHANE；blot time 2 s, wait time 0 s, blot force -1	分辨率 2.13 Å
9ES7 Cryo-EM structure of Spinacia oleracea cytochrome b6f complex with water molecules at 1.94 A resolution 提交 2024-03-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：18-meric	链 B 1–160（160 aa）链 J 1–160（160 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 CLA CHLOROPHYLL A × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 1.94 Å
9ES8 Cryo-EM structure of Spinacia oleracea cytochrome b6f with decylplastoquinone bound at plastoquionol reduction site 提交 2024-03-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：octadecameric	链 B 1–160（160 aa）链 J 1–160（160 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 A1H65 Decylplastoquinone × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 CLA CHLOROPHYLL A × 2 UMQ UNDECYL-MALTOSIDE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7；final concentration of plastocyanin was 0.34 mM cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.24 Å
9ES9 Cryo-EM structure of Spinacia oleracea cytochrome b6f complex with inhibitor DBMIB bound at plastoquinol oxidation site 提交 2024-03-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：18-meric	链 B 1–160（160 aa）链 J 1–160（160 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 BNT 2,5-DIBROMO-3-ISOPROPYL-6-METHYLBENZO-1,4-QUINONE × 2 CLA CHLOROPHYLL A × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.33 Å

共 6 个其他 PDB 条目、6 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	PETD_SPIOL
Isoform	—
PDB实体	2
链与序列区间	作者链 B； PDB构建体 1–160； UniProt 1–160 作者链 J； PDB构建体 1–160； UniProt 1–160

Cytochrome f

物种未注明

UniProt P16013

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白异源复合物异源复合物蛋白 × 17 PDB 声明：17-meric（17）与蛋白拷贝数一致	链 C； UniProt 36–320 链 K； UniProt 36–320	未记录	Cytochrome b6 × 2 （P00165） Cytochrome b6-f complex subunit 4 × 2 （P00166） Cytochrome b6-f complex iron-sulfur subunit, chloroplastic × 2 （P08980） Cytochrome b6-f complex subunit 6 × 2 （Q9M3L0） Cytochrome b6-f complex subunit 7 × 2 （P80883） Cytochrome b6-f complex subunit 5 × 2 （P69461） Cytochrome b6-f complex subunit 8 × 2 （P61045） Plastocyanin, chloroplastic × 1 （P00289） HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 7 CLA CHLOROPHYLL A × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 BCR BETA-CAROTENE × 2 CU COPPER (II) ION × 1	ELECTRON MICROSCOPY cryo-EM缓冲液：pH 7.5 cryo-EM玻璃化条件：冷冻剂 ETHANE	分辨率 3.18 Å

数据库中的同蛋白其他状态

其他 PDB	相对当前条目 9TGG	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
6RQF 3.6 Angstrom cryo-EM structure of the dimeric cytochrome b6f complex from Spinacia oleracea with natively bound thylakoid lipids and plastoquinone molecules 提交 2019-05-15	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 16 PDB 声明：hexadecameric	链 C 36–320（285 aa）链 K 36–320（285 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 BCR BETA-CAROTENE × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 6PL (4S,7R)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE × 3 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 PGV (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.58 Å
7QRM Cryo-EM structure of catalytically active Spinacia oleracea cytochrome b6f in complex with endogenous plastoquinones at 2.7 A resolution 提交 2022-01-11	构建体不同聚集状态不同配体/离子不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 16 PDB 声明：hexadecameric	链 C 1–320（320 aa）链 K 1–320（320 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 7 UMQ UNDECYL-MALTOSIDE × 10 PGT (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.5 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.70 Å
7ZYV Cryo-EM structure of catalytically active Spinacia oleracea cytochrome b6f in complex with endogenous plastoquinones at 2.13 A resolution 提交 2022-05-25	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：octadecameric	链 C 1–320（320 aa）链 K 1–320（320 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 UMQ UNDECYL-MALTOSIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8；Buffer and the diluted protein sample were filtered through a 0.22 um syringe filter. Prior to freezing, protein sample was concentrated on centrifugal filter units with 50K MWCO. cryo-EM玻璃化条件冷冻剂 ETHANE；blot time 2 s, wait time 0 s, blot force -1	分辨率 2.13 Å
9ES7 Cryo-EM structure of Spinacia oleracea cytochrome b6f complex with water molecules at 1.94 A resolution 提交 2024-03-25	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：18-meric	链 C 1–320（320 aa）链 K 1–320（320 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 CLA CHLOROPHYLL A × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 1.94 Å
9ES8 Cryo-EM structure of Spinacia oleracea cytochrome b6f with decylplastoquinone bound at plastoquionol reduction site 提交 2024-03-25	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：octadecameric	链 C 1–320（320 aa）链 K 1–320（320 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 A1H65 Decylplastoquinone × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 CLA CHLOROPHYLL A × 2 UMQ UNDECYL-MALTOSIDE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7；final concentration of plastocyanin was 0.34 mM cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.24 Å
9ES9 Cryo-EM structure of Spinacia oleracea cytochrome b6f complex with inhibitor DBMIB bound at plastoquinol oxidation site 提交 2024-03-25	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：18-meric	链 C 1–320（320 aa）链 K 1–320（320 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 BNT 2,5-DIBROMO-3-ISOPROPYL-6-METHYLBENZO-1,4-QUINONE × 2 CLA CHLOROPHYLL A × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.33 Å

共 6 个其他 PDB 条目、6 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	CYF_SPIOL
Isoform	—
PDB实体	3
链与序列区间	作者链 C； PDB构建体 1–285； UniProt 36–320 作者链 K； PDB构建体 1–285； UniProt 36–320

Cytochrome b6-f complex iron-sulfur subunit, chloroplastic

物种未注明

UniProt P08980

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白异源复合物异源复合物蛋白 × 17 PDB 声明：17-meric（17）与蛋白拷贝数一致	链 D； UniProt 52–230 链 L； UniProt 52–230	未记录	Cytochrome b6 × 2 （P00165） Cytochrome b6-f complex subunit 4 × 2 （P00166） Cytochrome f × 2 （P16013） Cytochrome b6-f complex subunit 6 × 2 （Q9M3L0） Cytochrome b6-f complex subunit 7 × 2 （P80883） Cytochrome b6-f complex subunit 5 × 2 （P69461） Cytochrome b6-f complex subunit 8 × 2 （P61045） Plastocyanin, chloroplastic × 1 （P00289） HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 7 CLA CHLOROPHYLL A × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 BCR BETA-CAROTENE × 2 CU COPPER (II) ION × 1	ELECTRON MICROSCOPY cryo-EM缓冲液：pH 7.5 cryo-EM玻璃化条件：冷冻剂 ETHANE	分辨率 3.18 Å

数据库中的同蛋白其他状态

其他 PDB	相对当前条目 9TGG	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
1RFS RIESKE SOLUBLE FRAGMENT FROM SPINACH 提交 1997-08-14	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 109–247（139 aa）片段：SOLUBLE FRAGMENT, C-TERMINAL RESIDUES 41 - 179	未记录	FES FE2/S2 (INORGANIC) CLUSTER × 1	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 1.83 Å R-free 0.220
6RQF 3.6 Angstrom cryo-EM structure of the dimeric cytochrome b6f complex from Spinacia oleracea with natively bound thylakoid lipids and plastoquinone molecules 提交 2019-05-15	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 16 PDB 声明：hexadecameric	链 D 52–230（179 aa）链 L 52–230（179 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 BCR BETA-CAROTENE × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 6PL (4S,7R)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE × 3 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 PGV (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.58 Å
7QRM Cryo-EM structure of catalytically active Spinacia oleracea cytochrome b6f in complex with endogenous plastoquinones at 2.7 A resolution 提交 2022-01-11	构建体不同聚集状态不同配体/离子不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 16 PDB 声明：hexadecameric	链 D 1–230（230 aa）链 L 1–230（230 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 7 UMQ UNDECYL-MALTOSIDE × 10 PGT (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.5 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.70 Å
7ZYV Cryo-EM structure of catalytically active Spinacia oleracea cytochrome b6f in complex with endogenous plastoquinones at 2.13 A resolution 提交 2022-05-25	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：octadecameric	链 D 1–230（230 aa）链 L 1–230（230 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 UMQ UNDECYL-MALTOSIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8；Buffer and the diluted protein sample were filtered through a 0.22 um syringe filter. Prior to freezing, protein sample was concentrated on centrifugal filter units with 50K MWCO. cryo-EM玻璃化条件冷冻剂 ETHANE；blot time 2 s, wait time 0 s, blot force -1	分辨率 2.13 Å
9ES7 Cryo-EM structure of Spinacia oleracea cytochrome b6f complex with water molecules at 1.94 A resolution 提交 2024-03-25	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：18-meric	链 D 1–230（230 aa）链 L 1–230（230 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 CLA CHLOROPHYLL A × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 1.94 Å
9ES8 Cryo-EM structure of Spinacia oleracea cytochrome b6f with decylplastoquinone bound at plastoquionol reduction site 提交 2024-03-25	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：octadecameric	链 D 1–230（230 aa）链 L 1–230（230 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 A1H65 Decylplastoquinone × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 CLA CHLOROPHYLL A × 2 UMQ UNDECYL-MALTOSIDE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7；final concentration of plastocyanin was 0.34 mM cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.24 Å
9ES9 Cryo-EM structure of Spinacia oleracea cytochrome b6f complex with inhibitor DBMIB bound at plastoquinol oxidation site 提交 2024-03-25	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：18-meric	链 D 1–230（230 aa）链 L 1–230（230 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 BNT 2,5-DIBROMO-3-ISOPROPYL-6-METHYLBENZO-1,4-QUINONE × 2 CLA CHLOROPHYLL A × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.33 Å

共 7 个其他 PDB 条目、7 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	UCRIA_SPIOL
Isoform	—
PDB实体	4
链与序列区间	作者链 D； PDB构建体 1–179； UniProt 52–230 作者链 L； PDB构建体 1–179； UniProt 52–230

Cytochrome b6-f complex subunit 6

物种未注明

UniProt Q9M3L0

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白异源复合物异源复合物蛋白 × 17 PDB 声明：17-meric（17）与蛋白拷贝数一致	链 E； UniProt 1–31 链 M； UniProt 1–31	未记录	Cytochrome b6 × 2 （P00165） Cytochrome b6-f complex subunit 4 × 2 （P00166） Cytochrome f × 2 （P16013） Cytochrome b6-f complex iron-sulfur subunit, chloroplastic × 2 （P08980） Cytochrome b6-f complex subunit 7 × 2 （P80883） Cytochrome b6-f complex subunit 5 × 2 （P69461） Cytochrome b6-f complex subunit 8 × 2 （P61045） Plastocyanin, chloroplastic × 1 （P00289） HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 7 CLA CHLOROPHYLL A × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 BCR BETA-CAROTENE × 2 CU COPPER (II) ION × 1	ELECTRON MICROSCOPY cryo-EM缓冲液：pH 7.5 cryo-EM玻璃化条件：冷冻剂 ETHANE	分辨率 3.18 Å

数据库中的同蛋白其他状态

其他 PDB	相对当前条目 9TGG	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
6RQF 3.6 Angstrom cryo-EM structure of the dimeric cytochrome b6f complex from Spinacia oleracea with natively bound thylakoid lipids and plastoquinone molecules 提交 2019-05-15	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 16 PDB 声明：hexadecameric	链 E 1–31（31 aa）链 M 1–31（31 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 BCR BETA-CAROTENE × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 6PL (4S,7R)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE × 3 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 PGV (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.58 Å
7QRM Cryo-EM structure of catalytically active Spinacia oleracea cytochrome b6f in complex with endogenous plastoquinones at 2.7 A resolution 提交 2022-01-11	聚集状态不同配体/离子不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 16 PDB 声明：hexadecameric	链 E 1–31（31 aa）链 M 1–31（31 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 7 UMQ UNDECYL-MALTOSIDE × 10 PGT (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.5 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.70 Å
7ZYV Cryo-EM structure of catalytically active Spinacia oleracea cytochrome b6f in complex with endogenous plastoquinones at 2.13 A resolution 提交 2022-05-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：octadecameric	链 E 1–31（31 aa）链 M 1–31（31 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 UMQ UNDECYL-MALTOSIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8；Buffer and the diluted protein sample were filtered through a 0.22 um syringe filter. Prior to freezing, protein sample was concentrated on centrifugal filter units with 50K MWCO. cryo-EM玻璃化条件冷冻剂 ETHANE；blot time 2 s, wait time 0 s, blot force -1	分辨率 2.13 Å
9ES7 Cryo-EM structure of Spinacia oleracea cytochrome b6f complex with water molecules at 1.94 A resolution 提交 2024-03-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：18-meric	链 E 1–31（31 aa）链 M 1–31（31 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 CLA CHLOROPHYLL A × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 1.94 Å
9ES8 Cryo-EM structure of Spinacia oleracea cytochrome b6f with decylplastoquinone bound at plastoquionol reduction site 提交 2024-03-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：octadecameric	链 E 1–31（31 aa）链 M 1–31（31 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 A1H65 Decylplastoquinone × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 CLA CHLOROPHYLL A × 2 UMQ UNDECYL-MALTOSIDE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7；final concentration of plastocyanin was 0.34 mM cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.24 Å
9ES9 Cryo-EM structure of Spinacia oleracea cytochrome b6f complex with inhibitor DBMIB bound at plastoquinol oxidation site 提交 2024-03-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：18-meric	链 E 1–31（31 aa）链 M 1–31（31 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 BNT 2,5-DIBROMO-3-ISOPROPYL-6-METHYLBENZO-1,4-QUINONE × 2 CLA CHLOROPHYLL A × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.33 Å

共 6 个其他 PDB 条目、6 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	PETL_SPIOL
Isoform	—
PDB实体	5
链与序列区间	作者链 E； PDB构建体 1–31； UniProt 1–31 作者链 M； PDB构建体 1–31； UniProt 1–31

Cytochrome b6-f complex subunit 7

物种未注明

UniProt P80883

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白异源复合物异源复合物蛋白 × 17 PDB 声明：17-meric（17）与蛋白拷贝数一致	链 F； UniProt 1–36 链 N； UniProt 1–36	未记录	Cytochrome b6 × 2 （P00165） Cytochrome b6-f complex subunit 4 × 2 （P00166） Cytochrome f × 2 （P16013） Cytochrome b6-f complex iron-sulfur subunit, chloroplastic × 2 （P08980） Cytochrome b6-f complex subunit 6 × 2 （Q9M3L0） Cytochrome b6-f complex subunit 5 × 2 （P69461） Cytochrome b6-f complex subunit 8 × 2 （P61045） Plastocyanin, chloroplastic × 1 （P00289） HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 7 CLA CHLOROPHYLL A × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 BCR BETA-CAROTENE × 2 CU COPPER (II) ION × 1	ELECTRON MICROSCOPY cryo-EM缓冲液：pH 7.5 cryo-EM玻璃化条件：冷冻剂 ETHANE	分辨率 3.18 Å

数据库中的同蛋白其他状态

其他 PDB	相对当前条目 9TGG	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
6RQF 3.6 Angstrom cryo-EM structure of the dimeric cytochrome b6f complex from Spinacia oleracea with natively bound thylakoid lipids and plastoquinone molecules 提交 2019-05-15	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 16 PDB 声明：hexadecameric	链 F 1–36（36 aa）链 N 1–36（36 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 BCR BETA-CAROTENE × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 6PL (4S,7R)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE × 3 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 PGV (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.58 Å
7QRM Cryo-EM structure of catalytically active Spinacia oleracea cytochrome b6f in complex with endogenous plastoquinones at 2.7 A resolution 提交 2022-01-11	聚集状态不同配体/离子不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 16 PDB 声明：hexadecameric	链 F 1–36（36 aa）链 N 1–36（36 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 7 UMQ UNDECYL-MALTOSIDE × 10 PGT (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.5 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.70 Å

共 2 个其他 PDB 条目、2 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	PETM_SPIOL
Isoform	—
PDB实体	6
链与序列区间	作者链 F； PDB构建体 1–36； UniProt 1–36 作者链 N； PDB构建体 1–36； UniProt 1–36

Cytochrome b6-f complex subunit 5

物种未注明

UniProt P69461

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白异源复合物异源复合物蛋白 × 17 PDB 声明：17-meric（17）与蛋白拷贝数一致	链 G； UniProt 1–37 链 O； UniProt 1–37	未记录	Cytochrome b6 × 2 （P00165） Cytochrome b6-f complex subunit 4 × 2 （P00166） Cytochrome f × 2 （P16013） Cytochrome b6-f complex iron-sulfur subunit, chloroplastic × 2 （P08980） Cytochrome b6-f complex subunit 6 × 2 （Q9M3L0） Cytochrome b6-f complex subunit 7 × 2 （P80883） Cytochrome b6-f complex subunit 8 × 2 （P61045） Plastocyanin, chloroplastic × 1 （P00289） HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 7 CLA CHLOROPHYLL A × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 BCR BETA-CAROTENE × 2 CU COPPER (II) ION × 1	ELECTRON MICROSCOPY cryo-EM缓冲液：pH 7.5 cryo-EM玻璃化条件：冷冻剂 ETHANE	分辨率 3.18 Å

数据库中的同蛋白其他状态

其他 PDB	相对当前条目 9TGG	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
6RQF 3.6 Angstrom cryo-EM structure of the dimeric cytochrome b6f complex from Spinacia oleracea with natively bound thylakoid lipids and plastoquinone molecules 提交 2019-05-15	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 16 PDB 声明：hexadecameric	链 G 1–37（37 aa）链 O 1–37（37 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 BCR BETA-CAROTENE × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 6PL (4S,7R)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE × 3 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 PGV (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.58 Å
7QRM Cryo-EM structure of catalytically active Spinacia oleracea cytochrome b6f in complex with endogenous plastoquinones at 2.7 A resolution 提交 2022-01-11	聚集状态不同配体/离子不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 16 PDB 声明：hexadecameric	链 G 1–37（37 aa）链 O 1–37（37 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 7 UMQ UNDECYL-MALTOSIDE × 10 PGT (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.5 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.70 Å
7ZYV Cryo-EM structure of catalytically active Spinacia oleracea cytochrome b6f in complex with endogenous plastoquinones at 2.13 A resolution 提交 2022-05-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：octadecameric	链 G 1–37（37 aa）链 O 1–37（37 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 UMQ UNDECYL-MALTOSIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8；Buffer and the diluted protein sample were filtered through a 0.22 um syringe filter. Prior to freezing, protein sample was concentrated on centrifugal filter units with 50K MWCO. cryo-EM玻璃化条件冷冻剂 ETHANE；blot time 2 s, wait time 0 s, blot force -1	分辨率 2.13 Å
9ES7 Cryo-EM structure of Spinacia oleracea cytochrome b6f complex with water molecules at 1.94 A resolution 提交 2024-03-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：18-meric	链 G 1–37（37 aa）链 O 1–37（37 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 CLA CHLOROPHYLL A × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 1.94 Å
9ES8 Cryo-EM structure of Spinacia oleracea cytochrome b6f with decylplastoquinone bound at plastoquionol reduction site 提交 2024-03-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：octadecameric	链 G 1–37（37 aa）链 O 1–37（37 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 A1H65 Decylplastoquinone × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 CLA CHLOROPHYLL A × 2 UMQ UNDECYL-MALTOSIDE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7；final concentration of plastocyanin was 0.34 mM cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.24 Å
9ES9 Cryo-EM structure of Spinacia oleracea cytochrome b6f complex with inhibitor DBMIB bound at plastoquinol oxidation site 提交 2024-03-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：18-meric	链 G 1–37（37 aa）链 O 1–37（37 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 BNT 2,5-DIBROMO-3-ISOPROPYL-6-METHYLBENZO-1,4-QUINONE × 2 CLA CHLOROPHYLL A × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.33 Å

共 6 个其他 PDB 条目、6 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	PETG_SPIOL
Isoform	—
PDB实体	7
链与序列区间	作者链 G； PDB构建体 1–37； UniProt 1–37 作者链 O； PDB构建体 1–37； UniProt 1–37

Cytochrome b6-f complex subunit 8

物种未注明

UniProt P61045

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白异源复合物异源复合物蛋白 × 17 PDB 声明：17-meric（17）与蛋白拷贝数一致	链 H； UniProt 1–29 链 P； UniProt 1–29	未记录	Cytochrome b6 × 2 （P00165） Cytochrome b6-f complex subunit 4 × 2 （P00166） Cytochrome f × 2 （P16013） Cytochrome b6-f complex iron-sulfur subunit, chloroplastic × 2 （P08980） Cytochrome b6-f complex subunit 6 × 2 （Q9M3L0） Cytochrome b6-f complex subunit 7 × 2 （P80883） Cytochrome b6-f complex subunit 5 × 2 （P69461） Plastocyanin, chloroplastic × 1 （P00289） HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 7 CLA CHLOROPHYLL A × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 BCR BETA-CAROTENE × 2 CU COPPER (II) ION × 1	ELECTRON MICROSCOPY cryo-EM缓冲液：pH 7.5 cryo-EM玻璃化条件：冷冻剂 ETHANE	分辨率 3.18 Å

数据库中的同蛋白其他状态

其他 PDB	相对当前条目 9TGG	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
6RQF 3.6 Angstrom cryo-EM structure of the dimeric cytochrome b6f complex from Spinacia oleracea with natively bound thylakoid lipids and plastoquinone molecules 提交 2019-05-15	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 16 PDB 声明：hexadecameric	链 H 1–29（29 aa）链 P 1–29（29 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 BCR BETA-CAROTENE × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 6PL (4S,7R)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE × 3 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 PGV (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.58 Å
7QRM Cryo-EM structure of catalytically active Spinacia oleracea cytochrome b6f in complex with endogenous plastoquinones at 2.7 A resolution 提交 2022-01-11	聚集状态不同配体/离子不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 16 PDB 声明：hexadecameric	链 H 1–29（29 aa）链 P 1–29（29 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 7 UMQ UNDECYL-MALTOSIDE × 10 PGT (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.5 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.70 Å
7ZYV Cryo-EM structure of catalytically active Spinacia oleracea cytochrome b6f in complex with endogenous plastoquinones at 2.13 A resolution 提交 2022-05-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：octadecameric	链 H 1–29（29 aa）链 P 1–29（29 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 CLA CHLOROPHYLL A × 2 UMQ UNDECYL-MALTOSIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8；Buffer and the diluted protein sample were filtered through a 0.22 um syringe filter. Prior to freezing, protein sample was concentrated on centrifugal filter units with 50K MWCO. cryo-EM玻璃化条件冷冻剂 ETHANE；blot time 2 s, wait time 0 s, blot force -1	分辨率 2.13 Å
9ES7 Cryo-EM structure of Spinacia oleracea cytochrome b6f complex with water molecules at 1.94 A resolution 提交 2024-03-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：18-meric	链 H 1–29（29 aa）链 P 1–29（29 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 CLA CHLOROPHYLL A × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 1.94 Å
9ES8 Cryo-EM structure of Spinacia oleracea cytochrome b6f with decylplastoquinone bound at plastoquionol reduction site 提交 2024-03-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：octadecameric	链 H 1–29（29 aa）链 P 1–29（29 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 A1H65 Decylplastoquinone × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 CLA CHLOROPHYLL A × 2 UMQ UNDECYL-MALTOSIDE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7；final concentration of plastocyanin was 0.34 mM cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.24 Å
9ES9 Cryo-EM structure of Spinacia oleracea cytochrome b6f complex with inhibitor DBMIB bound at plastoquinol oxidation site 提交 2024-03-25	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：18-meric	链 H 1–29（29 aa）链 P 1–29（29 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 2 BNT 2,5-DIBROMO-3-ISOPROPYL-6-METHYLBENZO-1,4-QUINONE × 2 CLA CHLOROPHYLL A × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 BCR BETA-CAROTENE × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.33 Å

共 6 个其他 PDB 条目、6 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	PETN_SPIOL
Isoform	—
PDB实体	8
链与序列区间	作者链 H； PDB构建体 1–29； UniProt 1–29 作者链 P； PDB构建体 1–29； UniProt 1–29

Plastocyanin, chloroplastic

物种未注明

UniProt P00289

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白异源复合物异源复合物蛋白 × 17 PDB 声明：17-meric（17）与蛋白拷贝数一致	链 Q； UniProt 70–168	未记录	Cytochrome b6 × 2 （P00165） Cytochrome b6-f complex subunit 4 × 2 （P00166） Cytochrome f × 2 （P16013） Cytochrome b6-f complex iron-sulfur subunit, chloroplastic × 2 （P08980） Cytochrome b6-f complex subunit 6 × 2 （Q9M3L0） Cytochrome b6-f complex subunit 7 × 2 （P80883） Cytochrome b6-f complex subunit 5 × 2 （P69461） Cytochrome b6-f complex subunit 8 × 2 （P61045） HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 4 UMQ UNDECYL-MALTOSIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 7 CLA CHLOROPHYLL A × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 BCR BETA-CAROTENE × 2 CU COPPER (II) ION × 1	ELECTRON MICROSCOPY cryo-EM缓冲液：pH 7.5 cryo-EM玻璃化条件：冷冻剂 ETHANE	分辨率 3.18 Å

数据库中的同蛋白其他状态

其他 PDB	相对当前条目 9TGG	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
1AG6 PLASTOCYANIN FROM SPINACH 提交 1997-04-02	突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 70–168（99 aa）	突变：G8D	CU COPPER (II) ION × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 4.4；pH 4.4	分辨率 1.60 Å R-free 0.224
1OOW The crystal structure of the spinach plastocyanin double mutant G8D/L12E gives insight into its low reactivity towards photosystem 1 and cytochrome f 提交 2003-03-04	突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 70–168（99 aa）	突变：G8D, L12E	CU COPPER (II) ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 4.5；300 K；32% PEG 3350, 0.25M MgCl2, 0.1M Na-ascorbate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K	分辨率 2.00 Å R-free 0.232
1TEF Crystal structure of the spinach plastocyanin mutants G8D/K30C/T69C and K30C/T69C- a study of the effect on crystal packing and thermostability from the introduction of a novel disulfide bond 提交 2004-05-25	突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 70–168（99 aa）	突变：G8D, K30C, T69C	CU COPPER (II) ION × 1 CL CHLORIDE ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；21% PEG 3350, 0.25M MgCl2, 0.1M Na-acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K	分辨率 1.90 Å R-free 0.204
1TEF Crystal structure of the spinach plastocyanin mutants G8D/K30C/T69C and K30C/T69C- a study of the effect on crystal packing and thermostability from the introduction of a novel disulfide bond 提交 2004-05-25	突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 2 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 B 70–168（99 aa）	突变：G8D, K30C, T69C	CU COPPER (II) ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；21% PEG 3350, 0.25M MgCl2, 0.1M Na-acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K	分辨率 1.90 Å R-free 0.204
1TEG Crystal structure of the spinach plastocyanin mutants G8D/K30C/T69C and K30C/T69C- a study of the effect on crystal packing and thermostability from the introduction of a novel disulfide bond 提交 2004-05-25	突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 70–168（99 aa）	突变：K30C, T69C	CU COPPER (II) ION × 1 CL CHLORIDE ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；21% PEG 3350, 0.25M MgCl2, 0.1M Na-acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K	分辨率 1.96 Å R-free 0.304
1TEG Crystal structure of the spinach plastocyanin mutants G8D/K30C/T69C and K30C/T69C- a study of the effect on crystal packing and thermostability from the introduction of a novel disulfide bond 提交 2004-05-25	突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 2 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 B 70–168（99 aa）	突变：K30C, T69C	CU COPPER (II) ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；21% PEG 3350, 0.25M MgCl2, 0.1M Na-acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K	分辨率 1.96 Å R-free 0.304
1YLB NMR solution structure of the reduced spinach plastocyanin 提交 2005-01-19	构建体不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 B 70–168（99 aa）片段：Reduced spinach plastocyanin (Residues 70-168)	未记录	CU1 COPPER (I) ION × 1	SOLUTION NMR NMR测量条件 pH 7.5；293 K；离子强度（mmCIF原始值）50 mM sodium phosphate；压力 ambient NMR样品组成 1-2 mM wild type reduced spinach plastocyanin, 50mM sodium phosphate buffer \| 90% H2O/10% D2O	分辨率未提供
2PCF THE COMPLEX OF CYTOCHROME F AND PLASTOCYANIN DETERMINED WITH PARAMAGNETIC NMR. BASED ON THE STRUCTURES OF CYTOCHROME F AND PLASTOCYANIN, 10 STRUCTURES 提交 1997-12-22	聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 70–168（99 aa）	未记录	CU COPPER (II) ION × 1 HEC HEME C × 1	SOLUTION NMR NMR测量条件 pH 6；300 K	分辨率未提供

共 6 个其他 PDB 条目、8 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	PLAS_SPIOL
Isoform	—
PDB实体	9
链与序列区间	作者链 Q； PDB构建体 1–99； UniProt 70–168

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	9tgg
沉积日期 deposition_date	2025-12-01
结构标题 title	Cryo-EM structure of Spinacia oleracea cytochrome b6f complex with bound plastocyanin
关键词 keywords	;b6f complex, photosynthesis, membrane protein, electron transport, proton transport, quinone catalysis, plastocyanin, OXIDOREDUCTASE ;; OXIDOREDUCTASE
实验方法 method	ELECTRON MICROSCOPY

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3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	46.11 Å
回转半径 Rg (电子) rg_electron	44.64 Å
零角强度 I(0) i0	644614000.00
分子量 molecular_weight	237190.0 kDa
排除体积 excluded_volume	308050 Å³
包络体积 envelope_volume	429740 Å³
水化壳体积 shell_volume	79142 Å³
包络直径 envelope_diameter	155.5 Å
壳层 Rg shell_rg	50.21 Å
包络 Rg envelope_rg	44.58 Å
形状 Rg shape_rg	44.62 Å
总 Rg total_rg	45.00 Å
总原子数 total_atoms	16731
残基数 n_residues	2001
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

▼

4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	147.2 Å
Rg (实空间) rg_real	46.01 Å
Rg 误差 (实空间) rg_real_error	1.17 Å
I(0) (实空间) i0_real	6.4460e+08
I(0) 误差 (实空间) i0_real_error	1.1730e+07
Rg (倒空间) rg_reciprocal	46.11 Å
I(0) (倒空间) i0_reciprocal	644700000.0000
解质量估计 total_estimate	0.6636
解质量评级 solution_quality	REASONABLE a REASONABLE solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	56.3 Å
偏度 Skewness skewness	0.240
峰度 Kurtosis kurtosis	-0.471
角度范围 angular_range	— – 0.1700 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	67930000.0000
实空间数据点数 n_real_points	35
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.939; Stabil: 1.000; Sysdev: 0.021; Positv: 1.000; Valcen: 0.991; Smooth: 0.750

▶

5. 晶体学与实验 5. Crystallography & Experiment

▶

6. 实体与聚合物信息 Entities & Polymers (18)

实体 1 Entity 1 polymer

描述 description	Cytochrome b6
分子量 formula_weight	24186.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	A,I
原子数 atom_count	16731

MSKVYDWFEERLEIQAIADDITSKYVPPHVNIFYCLGGITLTCFLVQVATGFAMTFYYRPTVTDAFASVQYIMTEVNFGWLIRSVHRWSASMMVLMMILHVFRVYLTGGFKKPRELTWVTGVVLGVLTASFGVTGYSLPWDQIGYWAVKIVTGVPDAIPVIGSPLVELLRGSASVGQSTLTRFYSLHTFVLPLLTAVFMLMHFLMIRKQGISGPL

实体 2 Entity 2 polymer

描述 description	Cytochrome b6-f complex subunit 4
分子量 formula_weight	17456.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	B,J

MGVTKKPDLNDPVLRAKLAKGMGHNYYGEPAWPNDLLYIFPVVILGTIACNVGLAVLEPSMIGEPADPFATPLEILPEWYFFPVFQILRTVPNKLLGVLLMASVPAGLLTVPFLENVNKFQNPFRRPVATTVFLVGTVVALWLGIGATLPIDKSLTLGLF

实体 3 Entity 3 polymer

描述 description	Cytochrome f
分子量 formula_weight	31357.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	C,K

YPIFAQQGYENPREATGRIVCANCHLANKPVDIEVPQAVLPDTVFEAVVRIPYDMQLKQVLANGKKGGLNVGAVLILPEGFELAPPDRISPEMKEKMGNLSFQSYRPNKQNILVIGPVPGQKYSEITFPILAPDPATKKDVHFLKYPIYVGGNRGRGQIYPDGSKSNNTVYNSTATGIVKKIVRKEKGGYEINIADASDGREVVDIIPRGPELLVSEGESIKLDQPLTSNPNVGGFGQGDAEVVLQDPLRIQGLLFFFASVILAQIFLVLKKKQFEKVQLSEMNF

实体 4 Entity 4 polymer

描述 description	Cytochrome b6-f complex iron-sulfur subunit, chloroplastic
分子量 formula_weight	18956.5 kDa
EC 编号 ec_number	7.1.1.6
来源方法 src_method	nat
拷贝数 entity_count	2

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	D,L

ATSIPADNVPDMQKRETLNLLLLGALSLPTGYMLLPYASFFVPPGGGAGTGGTIAKDALGNDVIAAEWLKTHAPGDRTLTQGLKGDPTYLVVESDKTLATFGINAVCTHLGCVVPFNAAENKFICPCHGSQYNNQGRVVRGPAPLSLALAHCDVDDGKVVFVPWTETDFRTGEAPWWSA

实体 5 Entity 5 polymer

描述 description	Cytochrome b6-f complex subunit 6
分子量 formula_weight	3452.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	E,M

MFTLTSYFGFLLAALTITSALFIGLNKIRLI

实体 6 Entity 6 polymer

描述 description	Cytochrome b6-f complex subunit 7
分子量 formula_weight	3774.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	F,N

NAAAEIFRIAAVMNGLTLVGVAIGFVLLRIEATVEE

实体 7 Entity 7 polymer

描述 description	Cytochrome b6-f complex subunit 5
分子量 formula_weight	4172.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	G,O

MIEVFLFGIVLGLIPITLAGLFVTAYLQYRRGDQLDL

实体 8 Entity 8 polymer

描述 description	Cytochrome b6-f complex subunit 8
分子量 formula_weight	3170.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	H,P

MDIVSLAWAALMVVFTFSLSLVVWGRSGL

实体 9 Entity 9 polymer

描述 description	Plastocyanin, chloroplastic
分子量 formula_weight	10420.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	Q

VEVLLGGGDGSLAFLPGDFSVASGEEIVFKNNAGFPHNVVFDEDEIPSGVDAAKISMSEEDLLNAPGETYKVTLTEKGTYKFYCSPHQGAGMVGKVTVN

实体 10 Entity 10 non-polymer

描述 description	PROTOPORPHYRIN IX CONTAINING FE
分子量 formula_weight	616.5 kDa
来源方法 src_method	syn
拷贝数 entity_count	4

实体 11 Entity 11 non-polymer

描述 description	HEME C
分子量 formula_weight	618.5 kDa
来源方法 src_method	syn
拷贝数 entity_count	4

实体 12 Entity 12 non-polymer

描述 description	UNDECYL-MALTOSIDE
分子量 formula_weight	496.6 kDa
来源方法 src_method	syn
拷贝数 entity_count	6

实体 13 Entity 13 non-polymer

描述 description	;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ;
分子量 formula_weight	749.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	7

实体 14 Entity 14 non-polymer

描述 description	CHLOROPHYLL A
分子量 formula_weight	893.5 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

实体 15 Entity 15 non-polymer

描述 description	FE2/S2 (INORGANIC) CLUSTER
分子量 formula_weight	175.8 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

实体 16 Entity 16 non-polymer

描述 description	1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
分子量 formula_weight	795.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

实体 17 Entity 17 non-polymer

描述 description	BETA-CAROTENE
分子量 formula_weight	536.9 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

实体 18 Entity 18 non-polymer

描述 description	COPPER (II) ION
分子量 formula_weight	63.5 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

▶

8. 引用文献 (1)

▶

晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	3.180 Å
最低分辨率 resolution_low	— Å
R 因子 r_factor	—
R_work r_work	—
R_free r_free	—

分辨率 resolution	3.18 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	87892

fit	/app/data/saxs_computation/tg/9tgg/9tgg.fit
pddf	/app/data/pr_computation/tg/9tgg/9tgg.pr.out
profile	/app/data/saxs_computation/tg/9tgg/9tgg.dat

Cryo-EM structure of Spinacia oleracea cytochrome b6f complex with bound plastocyanin

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Cytochrome b6

当前结构中的状态

数据库中的同蛋白其他状态

Cytochrome b6-f complex subunit 4

当前结构中的状态

数据库中的同蛋白其他状态

Cytochrome f

当前结构中的状态

数据库中的同蛋白其他状态

Cytochrome b6-f complex iron-sulfur subunit, chloroplastic

当前结构中的状态

数据库中的同蛋白其他状态

Cytochrome b6-f complex subunit 6

当前结构中的状态

数据库中的同蛋白其他状态

Cytochrome b6-f complex subunit 7

当前结构中的状态

数据库中的同蛋白其他状态

Cytochrome b6-f complex subunit 5

当前结构中的状态

数据库中的同蛋白其他状态

Cytochrome b6-f complex subunit 8

当前结构中的状态

数据库中的同蛋白其他状态

Plastocyanin, chloroplastic

当前结构中的状态

数据库中的同蛋白其他状态

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

2. 结构基本信息 2. Structure Basics

3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

5. 晶体学与实验 5. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

冷冻电镜 EM 3D

6. 实体与聚合物信息 Entities & Polymers (18)

实体 1 Entity 1 polymer

聚合物 Polymer

实体 2 Entity 2 polymer

聚合物 Polymer

实体 3 Entity 3 polymer

聚合物 Polymer

实体 4 Entity 4 polymer

聚合物 Polymer

实体 5 Entity 5 polymer

聚合物 Polymer

实体 6 Entity 6 polymer

聚合物 Polymer

实体 7 Entity 7 polymer

聚合物 Polymer

实体 8 Entity 8 polymer

聚合物 Polymer

实体 9 Entity 9 polymer

聚合物 Polymer

实体 10 Entity 10 non-polymer

实体 11 Entity 11 non-polymer

实体 12 Entity 12 non-polymer

实体 13 Entity 13 non-polymer

实体 14 Entity 14 non-polymer

实体 15 Entity 15 non-polymer

实体 16 Entity 16 non-polymer

实体 17 Entity 17 non-polymer

实体 18 Entity 18 non-polymer

8. 引用文献 (1)

9. 文件与曲线 (10)

Files (7)

Curves (3)