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9uih

CryoEM structure of Western equine encephalitis virus California VLP

Method: ELECTRON MICROSCOPY D_max: 168.4 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9uih
deposition_date	2025-04-15
title	CryoEM structure of Western equine encephalitis virus California VLP
keywords	WEEV, VLP, E2-E1 glycoproteins, VIRAL PROTEIN; VIRAL PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	52.78 Å
rg_electron	52.45 Å
i0	1139920000.00
molecular_weight	281340.0 kDa
excluded_volume	352110 Å³
envelope_volume	538570 Å³
shell_volume	85738 Å³
envelope_diameter	168.6 Å
shell_rg	55.44 Å
envelope_rg	52.08 Å
shape_rg	52.35 Å
total_rg	52.89 Å
total_atoms	19782
n_residues	2571
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	168.4 Å
rg_real	52.64 Å
rg_real_error	1.42 Å
i0_real	1.1400e+09
i0_real_error	2.2730e+07
rg_reciprocal	52.88 Å
i0_reciprocal	1140000000.0000
total_estimate	0.8844
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	67.4 Å
skewness	0.162
kurtosis	-0.491
angular_range	— – 0.1500 Å⁻¹
current_alpha	0.0000
highest_alpha	44930000.0000
n_real_points	31
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.924; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.983; Smooth: 0.739

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	Spike glycoprotein E1
formula_weight	47390.9 kDa
src_method	man
entity_count	3

Polymer

type	polypeptide(L)
strand_id	A,C,E
atom_count	19782

FEHATTVPNVPGIPYKALVERAGYAPLNLEITVVSSELTPSTNKEYVTCKFHTVIPSPQVKCCGSLECKASSKADYTCRVFGGVYPFMWGGAQCFCDSENTQLSEAYVEFAPDCTIDHAVALKVHTAALKVGLRIVYGNTTAHLDTFVNGVTPGSSRDLKVIAGPISAAFSPFDHKVVIRKGLVYNYDFPEYGAMKPGAFGDIQASSLYATDIVARTDIRLLKPSVKNIHVPYTQAVSGYEMWKNNSGRPLQETAPFGCKIEVEPLRASNCAYGHIPISIDIPDAAFVRSSESPTILEVSCTVADCIYSADFGGSLTLQYKADREGHCPVHSHSTTAVLKEATTHVTATGSITLHFSTSSPQANFIVSLCGKKTTCNAECKPPADHIIGEPHKVDQEFQAAVSKTSWNWLLALFGGASSLIVVGLIVLVCSSMLINTRR

Source

organism	Western equine encephalitis virus
ncbi_taxonomy_id	11039
host_organism	Homo sapiens

Entity 2 polymer

description	Spike glycoprotein E2
formula_weight	46534.1 kDa
src_method	man
entity_count	3

Polymer

type	polypeptide(L)
strand_id	B,D,F

SITDDFTLTSPYLGFCPYCRHSAPCFSPIKIENVWDESDDGSIRIQVSAQFGYNQAGTADVTKFRYMSYDHDHDIKEDSMEKIAISTSGPCRRLGHKGYFLLAQCPPGDSVTVSITSGASENSCTVEKKIRRKFVGREEYLFPPVHGKLLKCHIYDHLKETSAGYITMHRPGPHAYKSYLKEASGEVYIKPPSGKNVTYECKCGDYSTGIVSTQTKMNGCTKARQCIAYTRDQTKWVFNSPDLIRHTDHSVQGKLHIPFRLTPTVCRVPLAHTPTVTKWFKGITLHLTATRPTLLTTRKLGLRADATAEWITGTTSRNFSVGREGLEYVWGNHEPVRVWAQESAPGDPHGWPHEIIIHYYHRHPVYTVIVLCGVALAILVGTASSAACIAKARRDCLTPYALAPNATVPTALAVLCCI

Source

organism	Western equine encephalitis virus
ncbi_taxonomy_id	11039
host_organism	Homo sapiens

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6. Citations (1)

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resolution	3.050 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	3.05 Å
resolution_method	—
num_particles	292435

fit	/app/data/saxs_computation/ui/9uih/9uih.fit
pddf	/app/data/pr_computation/ui/9uih/9uih.pr.out
profile	/app/data/saxs_computation/ui/9uih/9uih.dat

CryoEM structure of Western equine encephalitis virus California VLP

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Refinement

EM 3D

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)