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9uio

CryoEM structure of Western equine encephalitis virus California VLP in complex with VLDLR-LA1-3

Method: ELECTRON MICROSCOPY D_max: 171.7 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9uio
deposition_date	2025-04-15
title	CryoEM structure of Western equine encephalitis virus California VLP in complex with VLDLR-LA1-3
keywords	WEEV, VLP, E2-E1 glycoproteins, VIRAL PROTEIN; VIRAL PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	53.23 Å
rg_electron	52.85 Å
i0	1401980000.00
molecular_weight	307820.0 kDa
excluded_volume	383210 Å³
envelope_volume	593670 Å³
shell_volume	92937 Å³
envelope_diameter	178.0 Å
shell_rg	56.67 Å
envelope_rg	52.53 Å
shape_rg	52.75 Å
total_rg	53.29 Å
total_atoms	21591
n_residues	2808
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	171.7 Å
rg_real	54.54 Å
rg_real_error	0.61 Å
i0_real	1.3890e+09
i0_real_error	2.3110e+07
rg_reciprocal	53.33 Å
i0_reciprocal	1402000000.0000
total_estimate	0.7030
solution_quality	REASONABLE a REASONABLE solution
n_peaks	1
r_peak_primary	68.7 Å
skewness	0.316
kurtosis	-0.241
angular_range	— – 0.1500 Å⁻¹
current_alpha	1.4160
highest_alpha	77790000.0000
n_real_points	31
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.930; Stabil: 0.913; Sysdev: 0.000; Positv: 1.000; Valcen: 0.986; Smooth: 0.654

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	Spike glycoprotein E1
formula_weight	47390.9 kDa
src_method	man
entity_count	3

Polymer

type	polypeptide(L)
strand_id	A,C,E
atom_count	21591

FEHATTVPNVPGIPYKALVERAGYAPLNLEITVVSSELTPSTNKEYVTCKFHTVIPSPQVKCCGSLECKASSKADYTCRVFGGVYPFMWGGAQCFCDSENTQLSEAYVEFAPDCTIDHAVALKVHTAALKVGLRIVYGNTTAHLDTFVNGVTPGSSRDLKVIAGPISAAFSPFDHKVVIRKGLVYNYDFPEYGAMKPGAFGDIQASSLYATDIVARTDIRLLKPSVKNIHVPYTQAVSGYEMWKNNSGRPLQETAPFGCKIEVEPLRASNCAYGHIPISIDIPDAAFVRSSESPTILEVSCTVADCIYSADFGGSLTLQYKADREGHCPVHSHSTTAVLKEATTHVTATGSITLHFSTSSPQANFIVSLCGKKTTCNAECKPPADHIIGEPHKVDQEFQAAVSKTSWNWLLALFGGASSLIVVGLIVLVCSSMLINTRR

Source

organism	Western equine encephalitis virus
ncbi_taxonomy_id	11039
host_organism	Homo sapiens

Entity 2 polymer

description	Spike glycoprotein E2
formula_weight	46534.1 kDa
src_method	man
entity_count	3

Polymer

type	polypeptide(L)
strand_id	B,D,F

SITDDFTLTSPYLGFCPYCRHSAPCFSPIKIENVWDESDDGSIRIQVSAQFGYNQAGTADVTKFRYMSYDHDHDIKEDSMEKIAISTSGPCRRLGHKGYFLLAQCPPGDSVTVSITSGASENSCTVEKKIRRKFVGREEYLFPPVHGKLLKCHIYDHLKETSAGYITMHRPGPHAYKSYLKEASGEVYIKPPSGKNVTYECKCGDYSTGIVSTQTKMNGCTKARQCIAYTRDQTKWVFNSPDLIRHTDHSVQGKLHIPFRLTPTVCRVPLAHTPTVTKWFKGITLHLTATRPTLLTTRKLGLRADATAEWITGTTSRNFSVGREGLEYVWGNHEPVRVWAQESAPGDPHGWPHEIIIHYYHRHPVYTVIVLCGVALAILVGTASSAACIAKARRDCLTPYALAPNATVPTALAVLCCI

Source

organism	Western equine encephalitis virus
ncbi_taxonomy_id	11039
host_organism	Homo sapiens

Entity 3 polymer

description	Very low-density lipoprotein receptor
formula_weight	8783.6 kDa
src_method	man
entity_count	3

Polymer

type	polypeptide(L)
strand_id	R,S,T

KCEPSQFQCTNGRCITLLWKCDGDEDCVDGSDEKNCVKKTCAESDFVCNNGQCVPSRWKCDGDPDCEDGSDESPEQCHM

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 4 non-polymer

description	CALCIUM ION
formula_weight	40.1 kDa
src_method	syn
entity_count	6

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6. Citations (1)

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resolution	3.030 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	3.03 Å
resolution_method	—
num_particles	139762

fit	/app/data/saxs_computation/ui/9uio/9uio.fit
pddf	/app/data/pr_computation/ui/9uio/9uio.pr.out
profile	/app/data/saxs_computation/ui/9uio/9uio.dat

CryoEM structure of Western equine encephalitis virus California VLP in complex with VLDLR-LA1-3

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Refinement

EM 3D

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)