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9uvt

Cryo-EM structure of bradykinin B2 receptor (B2R)-BRIL/anti BRIL SRP2070 Fab antibody complex with icatibant

Method: ELECTRON MICROSCOPY D_max: 122.8 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9uvt
deposition_date	2025-05-11
title	Cryo-EM structure of bradykinin B2 receptor (B2R)-BRIL/anti BRIL SRP2070 Fab antibody complex with icatibant
keywords	GPCR, B2R, bradykinin, icatibant, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	36.63 Å
rg_electron	36.95 Å
i0	118092000.00
molecular_weight	59718.0 kDa
excluded_volume	58429 Å³
envelope_volume	105430 Å³
shell_volume	25749 Å³
envelope_diameter	124.0 Å
shell_rg	39.14 Å
envelope_rg	36.81 Å
shape_rg	36.93 Å
total_rg	37.10 Å
total_atoms	4529
n_residues	568
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	122.8 Å
rg_real	37.16 Å
rg_real_error	1.16 Å
i0_real	1.1810e+08
i0_real_error	2.0040e+06
rg_reciprocal	36.84 Å
i0_reciprocal	118100000.0000
total_estimate	0.6433
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	25.7 Å
skewness	0.448
kurtosis	-0.846
angular_range	— – 0.2150 Å⁻¹
current_alpha	0.0000
highest_alpha	6953000.0000
n_real_points	44
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.394; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.175; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	Heavy chain of anti-BRIL SRP2070 fab antibody
formula_weight	31421.2 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	H
atom_count	4529

MEAPAQLLFLLLLWLPSSRAMQIQLQQSGPELVKPGASVKISCKASGYTFTDFYINWMKQRPGQGLEWIGWIFPGSGNTHYNEKFKGKATLIVDTSSSTAFMQLNSLTSEDSAVYFCTRPVSYYYDFDYWGQGTTLTVSSAKTTAPSVYPLAPVCGDTSGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGCLEVLFQGPGHHHHHHHHSAWSHPQFEKGGGSGGGGSGGSAWSHPQFEK

Source

organism	Mus musculus
ncbi_taxonomy_id	10090
host_organism	Cricetulus griseus

Entity 2 polymer

description	Light chain of anti-BRIL SRP2070 fab antibody
formula_weight	26065.0 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	L

MEAPAQLLFLLLLWLPSSRAMDIVLTQSPATLSVTPGDRVSLSCRASQSVSNYLHWYQQKSHESPRLLIKYASQSISGIPSRFSGSGSGTDFTLSINSVETEDFGMYFCQQSNSWPLTFGAGTKLELRRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC

Source

organism	Mus musculus
ncbi_taxonomy_id	10090
host_organism	Cricetulus griseus

Entity 3 polymer

description	B2 bradykinin receptor,Soluble cytochrome b562
formula_weight	54839.9 kDa
mutation	M258W/H353I/R357L
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A

GPLSVREDSVPTTASFSADMLNVTLQGPTLNGTFAQSKCPQVEWLGWLNTIQPPFLWVLFVLATLENIFVLSVFCLHKSSCTVAEIYLGNLAAADLILACGLPFWAITISNNFDWLFGETLCRVVNAIISMNLYSSICFLMLVSIDRYLALVKTMSMGRMRGVRWAKLYSLVIWGCTLLLSSPMLVFRTMKEYSDEGHNVTACVISYPSLIWEVFTNMLLNVVGFLLPLSVITFCTMQIMQVLADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLKEIQTERRATVLVLVVLLLFIICWLPFQISTFLDTLHRLGILSSCQDERIIDVITQIASFMAYSNSCLNPLVYVIVGKRFRKKSWEVYQGVCQKGGCRSEPIQMENSMGTLRTSISVERQIHKLQDWAGSRQLEVLFQ

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 4 polymer

description	icatibant
formula_weight	1307.5 kDa
src_method	syn
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

RRPPGASXXR

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.290 Å
resolution_low	3.290 Å
r_factor	—
r_work	0.3776
r_free	—

resolution	3.29 Å
resolution_method	—
num_particles	187522

fit	/app/data/saxs_computation/uv/9uvt/9uvt.fit
pddf	/app/data/pr_computation/uv/9uvt/9uvt.pr.out
profile	/app/data/saxs_computation/uv/9uvt/9uvt.dat

Cryo-EM structure of bradykinin B2 receptor (B2R)-BRIL/anti BRIL SRP2070 Fab antibody complex with icatibant

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 polymer

Polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)