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9ya1

Cryo-EM structure of intraconoidal microtubule 2 (ICMT2) from Toxoplasma gondii (8-nm repeat)

Method: ELECTRON MICROSCOPY D_max: 337.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9ya1
deposition_date	2025-09-15
title	Cryo-EM structure of intraconoidal microtubule 2 (ICMT2) from Toxoplasma gondii (8-nm repeat)
keywords	;Tubulin, Microtubule, Microtubule Inner Protein, Microtubule-associated Protein, Toxoplasma gondii, conoid, conoid fiber, intraconoidal microtubule, ICMT, STRUCTURAL PROTEIN ;; STRUCTURAL PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	— Å
rg_electron	120.00 Å
i0	147455000000.00
molecular_weight	3228200.0 kDa
excluded_volume	4009500 Å³
envelope_volume	7180200 Å³
shell_volume	478540 Å³
envelope_diameter	356.3 Å
shell_rg	136.50 Å
envelope_rg	110.00 Å
shape_rg	120.00 Å
total_rg	120.00 Å
total_atoms	226608
n_residues	28687
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	337.5 Å
rg_real	120.80 Å
rg_real_error	1.03 Å
i0_real	1.4390e+11
i0_real_error	2.7340e+09
rg_reciprocal	127.00 Å
i0_reciprocal	150600000000.0000
total_estimate	0.8919
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	181.7 Å
skewness	-0.143
kurtosis	-0.715
angular_range	— – 0.0650 Å⁻¹
current_alpha	1.7240
highest_alpha	3589000000.0000
n_real_points	14
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.942; Stabil: 0.932; Sysdev: 1.000; Positv: 1.000; Valcen: 0.981; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (11)

Entity 1 polymer

description	ICMAP4, TGME49_225340
formula_weight	230114.2 kDa
src_method	nat
entity_count	2

Polymer

type	polypeptide(L)
strand_id	1,2
atom_count	226608

MLSRMPEPACGSSYLGPPSLNLPSFPSGSCGATANSALRRLPEPCREQLERDLAVFARGSTSGVPPLPNASNKPVCPAAGQARMDRVKHRDSERSLSSQKFEGNEWGKKKRVFETETPDLTGHLRPVPGDSLRATGQLADNAAYFAPYSLTSRGKGPDTRDSDAENSGLEELADIGCGWEETESDAQLGGLSDAGSVSTAAPTDTPAALPGLEGLRRQTDDLNEWLDHSIRTLLHAARYQLSETADSDHPSEADAVEAARLEYQEALEKIQDARLKDRAKSDVHIAKLQSQLQKAQAECQNLQSTITEKDALLRQLQAHVKALRSGRASKSVSEAQPTEVDALFANLLNPDEGEAFAEDNPVANLTQASQRLAQNVTGGPKERKNAGAPTPADVLKTEFELKLQSLRSIKGSNAPALQEWLRVFVSSWKTLQTRLKSVSIPEKPTEKVLQLAKEKIEKERALTFKALVQVQQECLRVMKQVFETPWRAEELARENKEQTAKISLLSSDVEKTREHTAVLAAELKRRGRLIANLEEAVHMLSENLSLCQEDLSTRFEQVLESREKWKEAETELQRTQATLKVTKKEAAKLETHLEEVLGQYRASQRHARDLQVQLEDRTSCLHGVQDDLNRFEKANAELADDLHATRIELTKTAIELHAEQLAASQERSSEISEWKERQRRIEENILEALKKSEPKAASESSRGFPIRIHSPRTRPTGAFSRLRSSRRSPPKTRRGLTRLVSAVARTGEVERKLQENAEIVAGAKQATAGARDKGSLENLDETWKSIMQTVKKVERQVQSVYSSRRSHTVFARELRGLDRLLEGLEPQFVRAHKAFESLKLQASGEAAARFHQASELRAKDVAQAPLEANIRSTRPKGDQALRWADNLSGKLLGKKNQGRRSRFGKKMETRGLAVTRRGSRSSADFKREHSDSAWANVNDSDDERERNVPLSPSASATLKEESSRSEGTDSAEREFASSLSKSRSFVSEDRGRDAFPGFQGLQKKLRRFSSTGSTPLSDDSYSFSGVNRRHRRNRSVPPGRSPSQRNGDHAAAPLVHEVKRLEGELKRAQEIIKEIEETLLVPQDYFRKMREVTEENIRLNLLVCSLRADIEHCKVDRQDLESFHQVRVDYAQFQAETEKRIRDLEKQLKVEAAAARRSSLPDAAVRDAAGVARKKLENLRVEHQLEIRTLRDMNALEVERMRQNYEREISSLQQMIQTVKDNTESEYKKKLQLLQLQKETTMEKFRSELETQLRETKREYERELQEKQKDKVEALYKAEIAQQAALTEASRDFNKRLKEVEKHYEDRLLEHQVHVVKERDGLIARFKTKIENLTSQLEEQKEARWKAREAQDQAEVERKRHLAELERQLRVETLEKDELREALERQTSRAESLMEELGTVRREGGSPNIGRLADRGCQVEPTVRSRGTQVEDTVMEYIRECTEISEEAMQGDETGRVRSRVRDMLDERNGNLRGRCLLRHEPTFLSSPPAEGLCSRKESGEKSRKASVLEVEDDASIDGAVQAVENGVLKTASKAGTTTPSEGKGLKMLGTRTSIGCGVEKEDQAGKKEADLFSKVADVAFRAKAGSVGPFQRRGSEAEQKDQQADTSGQSRGERGAEISERNTTSDGLGSKDPSGRDKSPAVTGMKHYRDIKETPSIDKKLHADEDEGRQARDLSRSSSRDGPRSSSRDFDSFAVVRSKALEVLRRQRADPSDWEGCENVEKDHFGSRERHSNGEEFKTQGNVGRGSLRQEPFTDGVYHDRQQRFSEKEPAKPMFTSLADPSVRRHFKEEEERRKFQEKAEEEILRLLKRAAECSEEDLKREERSEKATEKGSRLFSGEEVRFFPASLDSELERLKERLRPGNSVLKAVRDTGAFSKFGSDDNSARSDRSRHNERRSSLSGAGTGVDDTFGNSRADNLRVEPASRRHTGEYARGTSFHGNRRPETVERQEKLVAGRSLRTSLIETLSATSHQIHVERSAVKCGLDHMLQRQEHSGWFPGRTENSKESGGTQATISEQMRALESLEMLSRRVRTGRRDAKQ

Entity 2 polymer

description	Microtubule associated protein SPM1
formula_weight	38845.8 kDa
src_method	nat
entity_count	11

Polymer

type	polypeptide(L)
strand_id	A,B,C,D,E,F,G,H,I,J,K

MSGGNSNTPKKLPSEEGSDYGYPQKPQKYLPKSEQAEPDYSACCKGNDAYKGASHGTVQFSHPEEAQKYAGAAAGAETIQRGRERVAADRQPRAAGDVPARRLHLSDVDEAHRGQSPSRHPGYCVEELCTCGMHKCIPSRAPVPFTGSTQYRQEFVPKPLPPPTQVSQVTLPPSLPFEAESSYRTEFVAKPLPPPAKFSEVKLPPTLPFHGESAYRTDYVPKPLPEVAKPVEVKLPPTLPFNAQSCYRSEYVAKPLPPPVQTVEVKLPPSLPFEGSTHYRDEFQVKPLPPATKVTEVKLPPSLPFDATSMYRSDYVAKSNPICPVSKLPQYPAATYPQNHVFWDPDTKQWY

Entity 3 polymer

description	Tubulin alpha chain
formula_weight	50166.6 kDa
src_method	nat
entity_count	26

Polymer

type	polypeptide(L)
strand_id	A0,A2,A4,A6,A8,B0,B2,B4,B6,B8,C0,C2,C4,C6,C8,D0,D2,D4,D6,D8,E0,E2,E4,E6,E8,F0

MREVISIHVGQAGIQIGNACWELFCLEHGIQPDGQMPSDKTIGGGDDAFNTFFSETGAGKHVPRCVFLDLEPTVVDEVRTGTYRHLFHPEQLISGKEDAANNFARGHYTIGKEIVDLSLDRIRKLADNCTGLQGFLMFNAVGGGTGSGLGCLLLERLSVDYGKKSKLNFCSWPSPQVSTAVVEPYNSVLSTHSLLEHTDVAVMLDNEAIYDICRRNLDIERPTYTNLNRLIAQVISSLTASLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPIISAEKAYHEQLSVAEITNSAFEPASMMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPPTVVPGGDLAKVMRAVCMISNSTAIAEVFSRMDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGIETAEGEGEEEGYGDEY

Entity 4 polymer

description	Tubulin beta chain
formula_weight	50119.1 kDa
src_method	nat
entity_count	26

Polymer

type	polypeptide(L)
strand_id	A1,A3,A5,A7,A9,B1,B3,B5,B7,B9,C1,C3,C5,C7,C9,D1,D3,D5,D7,D9,E1,E3,E5,E7,E9,F1

MREIVHVQGGQCGNQIGAKFWEVISDEHGIDPTGTYCGDSDLQLERINVFYNEATGGRFVPRAILMDLEPGTMDSVRAGPFGQLFRPDNFVFGQTGAGNNWAKGHYTEGAELIDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGSGMGTLLISKVREEYPDRIMETFSVFPSPKVSDTVVEPYNATLSVHQLVENADEVQVIDNEALYDICFRTLKLTTPTYGDLNHLVSAAMSGVTCCLRFPGQLNSDLRKLAVNLIPFPRLHFFLIGFAPLTSRGSQQYRALSVPELTQQMFDAKNMMCASDPRHGRYLTASAMFRGRMSTKEVDEQMLNVQNKNSSYFVEWIPNNMKSSVCDIPPKGLKMSVTFVGNSTAIQEMFKRVSDQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEGEFDEEEGEMGAEEGA

Entity 5 polymer

description	TLAP3 (apical cap protein AC5), TGME49_235380
formula_weight	65286.1 kDa
src_method	nat
entity_count	3

Polymer

type	polypeptide(L)
strand_id	O,P,R

MPRPGVFELNYGEESRHSPVPKDPSTVTYGDASVGVVHHPLPAAGMYRQPCAFPHGSGFSKQSNETAFPDYPSTEHVAQRYVPPMDDKRQWAGHTSYYAPATMEQRGYQDARYFGAELMPKSQGGENPQNHPIMPSEAAEYGPRKPDCGRLIAAPHEGQVNNLSQVDGRICGTLPVIDNQSFQVEPCFRAPTIATGIPRLQTMKGRDAFAQDATRQRALQGHRTADLLKTPFDYDLFHRTRLPPSAGASIQAAGKEIDWSEKKLFRKAVVSTVFASDQVAERLRQDLPSPNTRDGQRTNADHGNRRNWSENIESLLRQATPVESSAENDPIFTAKVEERSFDSSLLYHGCRHLKKRSAFGHTPDAVAQLLRSSAEAGKGERPLVPSRPSRPSQHDSTQLKRVLSYHPDPEADSKGCFEQRARLRRSQLVTAKNSCRWGVNVFGYDLSPPRRNRLYALRDHLDSKLVPNQSTDDATHIKPRHTNVLSTSLHMSKLVPVTDLSPRPSFRYHADTGSLDATLLPVDAVPQERIGRRLISPPESSLQSNFVPSHEEVGRHKRFLVNSRDSLQGNMIPIVKECTRRRK

Entity 6 polymer

description	TLAP4 (thioredoxin-like associated protein), TGME49_201760
formula_weight	37360.2 kDa
src_method	nat
entity_count	4

Polymer

type	polypeptide(L)
strand_id	U,V,W,X

MERSGHLCKMEPWKQKVPQCLSLHGSNSLRGGKRTYNCNVLMDNWRNDRLDAHNDQKLTSWRIPHPLYTELPQGSKYETHYRQSYDYEDWARRPVRLSKYGLLGHDDSGAESWTTETRSEYEPPRLRRDQLKAAGQWPYGEDIYPRHVHGNEEFMCPHPMAIHVSRTHREDYIRSLTPRPAYERPPAGVPSSSGRLPGVPPYANDPISHHGGSQEQRHFCRMPCRNPSGSSSVQGTTGVDARASPAGSPVFAGQVPAQVKQEGGECGQGYAGIAATEDGLVDHGNSQTDRSAGQHCMLNASGGSERAHAAANTSTCPQRKESDPQPTVSWAAVPCQ

Entity 7 polymer

description	TRXL1, TGME49_232410
formula_weight	24939.3 kDa
src_method	nat
entity_count	22

Polymer

type	polypeptide(L)
strand_id	a,b,c,d,e,f,g,h,i,j,m,n,o,p,q,r,s,t,u,v,w,x

MSQPVFASPLNVEKRRLNEERALMQAQKAGGEGVNIQLPPNYGDMDLILFPEGSLKNSNNTVIPQSHLKGKSVALYFADGADPKCASLLPFLLNYYRTMNEGGANQKIEIIFVSLDRDREAFESHRAHMPWLSIDLENPLTEILKRHFRVMKEYEVPTYGYGSRTGVPSVIVIGSDGREAQFLPICSGLEEGDRALLRWDWRNTKFASDQFHVRPTLLEQ

Entity 8 polymer

description	TRXL2, TGME49_225790
formula_weight	21687.9 kDa
src_method	nat
entity_count	2

Polymer

type	polypeptide(L)
strand_id	k,l

MLAADCFFGPDVVKRTQQGNYVPVRPDHFAGVSVALFFAKAGHSKCAQIVPVVRQFYKTTNFSGEKAVIEIIYVSLDKDEQDFERVRALMPWCSVEYKSCLRKKLIERYRVPNGELAFGTVRIPSTAIPLLIVIGPNGEEAGRMNFQQSDEFVLQRWDYRFNKWPGSAQRLRTLNDATDPWKKRLPQNV

Entity 9 non-polymer

description	GUANOSINE-5'-TRIPHOSPHATE
formula_weight	523.2 kDa
src_method	syn
entity_count	26

Entity 10 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	26

Entity 11 non-polymer

description	GUANOSINE-5'-DIPHOSPHATE
formula_weight	443.2 kDa
src_method	syn
entity_count	26

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6. Citations (1)

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resolution	3.52 Å
resolution_method	—
num_particles	55276

fit	/app/data/saxs_computation/ya/9ya1/9ya1.fit
pddf	/app/data/pr_computation/ya/9ya1/9ya1.pr.out
profile	/app/data/saxs_computation/ya/9ya1/9ya1.dat

Cryo-EM structure of intraconoidal microtubule 2 (ICMT2) from Toxoplasma gondii (8-nm repeat)

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

EM 3D

5. Entities & Polymer Info (11)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Entity 4 polymer

Polymer

Entity 5 polymer

Polymer

Entity 6 polymer

Polymer

Entity 7 polymer

Polymer

Entity 8 polymer

Polymer

Entity 9 non-polymer

Entity 10 non-polymer

Entity 11 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)