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9yxd

Crystal structure of recombinant human follicle stimulating hormone in complex with an anti-FSH alpha Fab

Method: X-RAY DIFFRACTION D_max: 123.5 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9yxd
deposition_date	2025-10-27
last_revision	2026-03-11
title	Crystal structure of recombinant human follicle stimulating hormone in complex with an anti-FSH alpha Fab
keywords	Gonadotropin, Glycoprotein hormone, cystine knot cytokine, GPCR signaling, HORMONE; HORMONE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	36.34 Å
rg_electron	36.42 Å
i0	113027000.00
molecular_weight	82735.0 kDa
excluded_volume	102400 Å³
envelope_volume	138680 Å³
shell_volume	34751 Å³
envelope_diameter	132.5 Å
shell_rg	39.26 Å
envelope_rg	35.95 Å
shape_rg	36.35 Å
total_rg	36.82 Å
total_atoms	5827
n_residues	743
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	123.5 Å
rg_real	36.68 Å
rg_real_error	1.48 Å
i0_real	1.1300e+08
i0_real_error	2.2250e+06
rg_reciprocal	36.47 Å
i0_reciprocal	113000000.0000
total_estimate	0.6044
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	36.5 Å
skewness	0.503
kurtosis	-0.369
angular_range	— – 0.2200 Å⁻¹
current_alpha	0.0000
highest_alpha	7501000.0000
n_real_points	45
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.817; Stabil: 1.000; Sysdev: 0.028; Positv: 1.000; Valcen: 0.737; Smooth: 0.583

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4. Crystallography & Experiment

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5. Entities & Polymer Info (10)

Entity 1 polymer

description	Glycoprotein hormones alpha chain
formula_weight	10217.8 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	6146

APDVQDCPECTLQENPFFSQPGAPILQCMGCCFSRAYPTPLRSKKTMLVQKNVTSESTCCVAKSYNRVTVMGGFKVENHTACHCSTCYYHKS

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 2 polymer

description	Follitropin subunit beta
formula_weight	12500.2 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

NSCELTNITIAIEKEECRFCISINTTWCAGYCYTRDLVYKDPARPKIQKTCTFKELVYETVRVPGCAHHADSLYTYPVATQCHCGKCDSDSTDCTVRGLGPSYCSFGEMKE

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 3 polymer

description	Fab light chain
formula_weight	24264.8 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	C

DIELTQSPDSLSVSLGQRATISCRASESVDSYGNSFMQWYQQKPGQPPKLLIYRASNLESGIPARFSGTGSRTDFTLTINPVEADDVATYYCQQSDEYPYMYTFGGGTKLEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC

Source

organism	Mus musculus
ncbi_taxonomy_id	10090
host_organism	Homo sapiens

Entity 4 polymer

description	Fab heavy chain
formula_weight	24301.1 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	D

QVQLQQSGAELVKPGASVKLSCKASDYTFTSYWMHWVKQRPGQGLEWIGEINPTNGRTYYNEKFKSKATLTVDKSSSTAYMQLSSLTSEDSAVYYCARRYGNSFDYWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT

Source

organism	Mus musculus
ncbi_taxonomy_id	10090
host_organism	Homo sapiens

Entity 5 polymer

description	Ig-like domain-containing protein
formula_weight	14075.4 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	K

HHHHHHQVQLQESGGGLVQPGGSLRLSCAASGRTISRYAMSWFRQAPGKEREFVAVARRSGDGAFYADSVQGRFTVSRDDAKNTVYLQMNSLKPEDTAVYYCAIDSDTFYSGSYDYWGQGTQVTVSS

Source

organism	Lama glama
ncbi_taxonomy_id	9844
host_organism	Escherichia coli BL21(DE3)

Entity 6 branched

description	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
formula_weight	424.4 kDa
src_method	man
entity_count	1

Entity 7 non-polymer

description	2-acetamido-2-deoxy-beta-D-glucopyranose
formula_weight	221.2 kDa
src_method	syn
entity_count	3

Entity 8 non-polymer

description	2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL
formula_weight	209.2 kDa
src_method	syn
entity_count	1

Entity 9 non-polymer

description	GLYCEROL
formula_weight	92.1 kDa
src_method	syn
entity_count	1

Entity 10 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	319

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6. Citations (1)

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length_a	76.010 Å
length_b	101.103 Å
length_c	161.240 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 21
int_tables_number	19
crystal_system	orthorhombic

resolution	2.290 Å
resolution_low	29.850 Å
r_factor	0.2030
r_work	0.2013
r_free	0.2351

fit	/app/data/saxs_computation/yx/9yxd/9yxd.fit
pddf	/app/data/pr_computation/yx/9yxd/9yxd.pr.out
profile	/app/data/saxs_computation/yx/9yxd/9yxd.dat

Crystal structure of recombinant human follicle stimulating hormone in complex with an anti-FSH alpha Fab

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (10)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 polymer

Polymer

Source

Entity 5 polymer

Polymer

Source

Entity 6 branched

Entity 7 non-polymer

Entity 8 non-polymer

Entity 9 non-polymer

Entity 10 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)