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9z2m

Ca2+-bound MthK WT in lipid nanodiscs composed of 14:1PC (75%) and POPG (25%)

Method: ELECTRON MICROSCOPY Dmax: 131.1 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Calcium-gated potassium channel MthK

Methanothermobacter thermautotrophicus

UniProt O27564

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain A； UniProt 1–336 Chain B； UniProt 1–336 Chain C； UniProt 1–336 Chain D； UniProt 1–336 Chain E； UniProt 1–336 Chain F； UniProt 1–336 Chain G； UniProt 1–336 Chain H； UniProt 1–336 Not recorded No other associated polymer ELECTRON MICROSCOPY cryo-EM buffer：pH 8.5；20 mM HEPES-KOH pH 8.5 100 mM KCl cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9Z2M Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1LNQ CRYSTAL STRUCTURE OF MTHK AT 3.3 A Deposited 2002-05-03 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Chain E 1–336（336 aa）
Chain F 1–336（336 aa）
Chain G 1–336（336 aa）
Chain H 1–336（336 aa）
Not recorded CA CALCIUM ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；PEGMME350, CaCl2, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 3.30 Å R-free 0.318
2AEF Crystal Structures of the MthK RCK Domain in Ca2+ bound form Deposited 2005-07-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 107–336（230 aa） Fragment：isoform Soluble
Chain B 107–336（230 aa） Fragment：isoform Soluble
Not recorded CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；PEG 4000, calcium chloride, Ammonium formate, sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.70 Å R-free 0.268
2AEJ Crystal Structures of the MthK RCK Domain in no Ca2+ bound form Deposited 2005-07-22 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 107–336（230 aa） Fragment：isoform Soluble
Chain B 107–336（230 aa） Fragment：isoform Soluble
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；PEG 4000, Ammonium formate, sodium acetate , pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.10 Å R-free 0.245
2AEM Crystal Structures of the MthK RCK Domain Deposited 2005-07-22 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 107–336（230 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；293 K；PEG 400, Ammonium sulfate, cacodylate , pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.80 Å R-free 0.263
2FY8 Crystal structure of MthK rck domain in its ligand-free gating-ring form Deposited 2006-02-07 Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 107–336（230 aa） Fragment：soluble isoform, residues 107-336
Chain B 107–336（230 aa） Fragment：soluble isoform, residues 107-336
Chain C 107–336（230 aa） Fragment：soluble isoform, residues 107-336
Chain D 107–336（230 aa） Fragment：soluble isoform, residues 107-336
Mutation：D184N Mutation：D184N Mutation：D184N Mutation：D184N No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；0.6 M Na/K PO4, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.79 Å R-free 0.254
2FY8 Crystal structure of MthK rck domain in its ligand-free gating-ring form Deposited 2006-02-07 Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain E 107–336（230 aa） Fragment：soluble isoform, residues 107-336
Chain F 107–336（230 aa） Fragment：soluble isoform, residues 107-336
Chain G 107–336（230 aa） Fragment：soluble isoform, residues 107-336
Chain H 107–336（230 aa） Fragment：soluble isoform, residues 107-336
Mutation：D184N Mutation：D184N Mutation：D184N Mutation：D184N No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；0.6 M Na/K PO4, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.79 Å R-free 0.254
2OGU Crystal structure of the isolated MthK RCK domain Deposited 2007-01-08 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 107–336（230 aa） Fragment：RCK domain: Residues 107-336
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；0.1M Sodium cacodylate pH 6.5, 1.3-1.5M Lithium sulfate, 2% Benzamidine-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 3.23 Å R-free 0.299
2OGU Crystal structure of the isolated MthK RCK domain Deposited 2007-01-08 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 107–336（230 aa） Fragment：RCK domain: Residues 107-336
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；0.1M Sodium cacodylate pH 6.5, 1.3-1.5M Lithium sulfate, 2% Benzamidine-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 3.23 Å R-free 0.299
3KXD Crystal structure of the mthk rck in complex with cadmium Deposited 2009-12-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 116–336（221 aa） Fragment：RCK DOMAIN
Chain B 116–336（221 aa） Fragment：RCK DOMAIN
Not recorded CD CADMIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.7；318 K；1M NH4-FORMATE, 10% PEG 4000, 0.1M NA ACETATE, PH 4.7, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 318K
Resolution 2.20 Å R-free 0.254
3LDC High resolution open MthK pore structure crystallized in 100 mM K+ Deposited 2010-01-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 18–99（82 aa） Fragment：MthK K+ channel, residues 28-99
Mutation：S68H, V77C K POTASSIUM ION × 20 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；3.0-3.5 M 1,6-Hexandiol, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.45 Å R-free 0.202
3LDD High resolution open MthK pore structure crystallized in 100 mM K+ and further soaked in 99 mM Na+/1 mM K+. Deposited 2010-01-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 18–99（82 aa） Fragment：MthK K+ channel, residues 28-99
Mutation：S68R, V77C K POTASSIUM ION × 20 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；3.0-3.5 M 1,6-Hexandiol, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.45 Å R-free 0.216
3LDE High resolution open MthK pore structure crystallized in 100 mM K+ and further soaked in 100 mM Na+. Deposited 2010-01-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 18–99（82 aa） Fragment：MthK K+ channel, residues 28-99
Mutation：S68H, V77C NA SODIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；3.0-3.5 M 1,6-Hexandiol, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.21 Å R-free 0.208
3OUS MthK channel pore T59A mutant Deposited 2010-09-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 18–99（82 aa）
Mutation：T59A K POTASSIUM ION × 20 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；1M 1,6-hexanediol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.75 Å R-free 0.236
3R65 MthK channel pore E92Q mutant Deposited 2011-03-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 18–99（82 aa） Fragment：CALCIUM-GATED POTASSIUM CHANNEL MTHK PORE (UNP Residues 18-99)
Mutation：E92Q K POTASSIUM ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；3.0-3.5 M 1,6-Hexandiol, 100 mM MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.80 Å R-free 0.225
3RBX MthK RCK domain D184N mutant, Ca2+-bound Deposited 2011-03-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 107–336（230 aa） Fragment：unp residues 107-336
Chain B 107–336（230 aa） Fragment：unp residues 107-336
Mutation：D184N Mutation：D184N CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.7；295 K；20% PEG 3350, 0.1 M CaCl2, 0.1 M MES, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K, pH 5.7
Resolution 2.80 Å R-free 0.274
3RBX MthK RCK domain D184N mutant, Ca2+-bound Deposited 2011-03-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 107–336（230 aa） Fragment：unp residues 107-336
Chain D 107–336（230 aa） Fragment：unp residues 107-336
Mutation：D184N Mutation：D184N CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.7；295 K；20% PEG 3350, 0.1 M CaCl2, 0.1 M MES, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K, pH 5.7
Resolution 2.80 Å R-free 0.274
3RBX MthK RCK domain D184N mutant, Ca2+-bound Deposited 2011-03-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain E 107–336（230 aa） Fragment：unp residues 107-336
Chain F 107–336（230 aa） Fragment：unp residues 107-336
Mutation：D184N Mutation：D184N CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.7；295 K；20% PEG 3350, 0.1 M CaCl2, 0.1 M MES, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K, pH 5.7
Resolution 2.80 Å R-free 0.274
3RBZ MthK channel, Ca2+-bound Deposited 2011-03-30 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Mutation：G85A, E92A, E96T, M107I Mutation：G85A, E92A, E96T, M107I Mutation：G85A, E92A, E96T, M107I Mutation：G85A, E92A, E96T, M107I CA CALCIUM ION × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；26% PEG 350-MME, 0.2 M CaCl2, 0.1 M MES, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 6.5
Resolution 3.40 Å R-free 0.288
4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 107–336（230 aa） Fragment：residues 115-336
Not recorded BA BARIUM ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
Resolution 3.11 Å R-free 0.242
4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 10 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain J 107–336（230 aa） Fragment：residues 115-336
Not recorded BA BARIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
Resolution 3.11 Å R-free 0.242
4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 11 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain K 107–336（230 aa） Fragment：residues 115-336
Not recorded BA BARIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
Resolution 3.11 Å R-free 0.242
4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 12 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain L 107–336（230 aa） Fragment：residues 115-336
Not recorded BA BARIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
Resolution 3.11 Å R-free 0.242
4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 13 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain M 107–336（230 aa） Fragment：residues 115-336
Not recorded BA BARIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
Resolution 3.11 Å R-free 0.242
4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 14 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain N 107–336（230 aa） Fragment：residues 115-336
Not recorded BA BARIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
Resolution 3.11 Å R-free 0.242
4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 15 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain O 107–336（230 aa） Fragment：residues 115-336
Not recorded BA BARIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
Resolution 3.11 Å R-free 0.242
4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 16 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain P 107–336（230 aa） Fragment：residues 115-336
Not recorded BA BARIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
Resolution 3.11 Å R-free 0.242
4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 107–336（230 aa） Fragment：residues 115-336
Not recorded BA BARIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
Resolution 3.11 Å R-free 0.242
4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 107–336（230 aa） Fragment：residues 115-336
Not recorded BA BARIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
Resolution 3.11 Å R-free 0.242
4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 107–336（230 aa） Fragment：residues 115-336
Not recorded BA BARIUM ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
Resolution 3.11 Å R-free 0.242
4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain E 107–336（230 aa） Fragment：residues 115-336
Not recorded BA BARIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
Resolution 3.11 Å R-free 0.242
4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain F 107–336（230 aa） Fragment：residues 115-336
Not recorded BA BARIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
Resolution 3.11 Å R-free 0.242
4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 7 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain G 107–336（230 aa） Fragment：residues 115-336
Not recorded BA BARIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
Resolution 3.11 Å R-free 0.242
4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 8 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain H 107–336（230 aa） Fragment：residues 115-336
Not recorded BA BARIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
Resolution 3.11 Å R-free 0.242
4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 9 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain I 107–336（230 aa） Fragment：residues 115-336
Not recorded BA BARIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
Resolution 3.11 Å R-free 0.242
4HYO Crystal Structure of MthK Pore Deposited 2012-11-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 11–101（91 aa） Fragment：unp residues 11-101
Chain B 11–101（91 aa） Fragment：unp residues 11-101
Chain C 11–101（91 aa） Fragment：unp residues 11-101
Chain D 11–101（91 aa） Fragment：unp residues 11-101
Mutation：S68H, V77C Mutation：S68H, V77C Mutation：S68H, V77C Mutation：S68H, V77C K POTASSIUM ION × 4 HEZ HEXANE-1,6-DIOL × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；3.5-4.0M 1,6-hexanediol, 100mM MES , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.65 Å R-free 0.181
4HYO Crystal Structure of MthK Pore Deposited 2012-11-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain E 11–101（91 aa） Fragment：unp residues 11-101
Chain F 11–101（91 aa） Fragment：unp residues 11-101
Chain G 11–101（91 aa） Fragment：unp residues 11-101
Chain H 11–101（91 aa） Fragment：unp residues 11-101
Mutation：S68H, V77C Mutation：S68H, V77C Mutation：S68H, V77C Mutation：S68H, V77C K POTASSIUM ION × 4 HEZ HEXANE-1,6-DIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；3.5-4.0M 1,6-hexanediol, 100mM MES , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.65 Å R-free 0.181
4HZ3 MthK pore crystallized in presence of TBSb Deposited 2012-11-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 11–101（91 aa） Fragment：unp residues 11-101
Chain B 11–101（91 aa） Fragment：unp residues 11-101
Chain C 11–101（91 aa） Fragment：unp residues 11-101
Chain D 11–101（91 aa） Fragment：unp residues 11-101
Mutation：S68H, V77C Mutation：S68H, V77C Mutation：S68H, V77C Mutation：S68H, V77C K POTASSIUM ION × 4 HEZ HEXANE-1,6-DIOL × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；3.5 4.0M 1,6-hexanediol, 100mM MES, 10mM TBSb , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.70 Å R-free 0.192
4HZ3 MthK pore crystallized in presence of TBSb Deposited 2012-11-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain E 11–101（91 aa） Fragment：unp residues 11-101
Chain F 11–101（91 aa） Fragment：unp residues 11-101
Chain G 11–101（91 aa） Fragment：unp residues 11-101
Chain H 11–101（91 aa） Fragment：unp residues 11-101
Mutation：S68H, V77C Mutation：S68H, V77C Mutation：S68H, V77C Mutation：S68H, V77C K POTASSIUM ION × 4 HEZ HEXANE-1,6-DIOL × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；3.5 4.0M 1,6-hexanediol, 100mM MES, 10mM TBSb , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.70 Å R-free 0.192
4L73 Ca2+-bound MthK RCK domain at 2.5 Angstrom Deposited 2013-06-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 107–336（230 aa） Fragment：RCK domain (UNP residues 107-336)
Chain B 107–336（230 aa） Fragment：RCK domain (UNP residues 107-336)
Not recorded CA CALCIUM ION × 5 NA SODIUM ION × 8 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；295 K；12% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 1 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.50 Å R-free 0.226
4L74 Ca2+-bound MthK RCK domain at 1.9 Angstrom with single ligand Deposited 2013-06-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 107–336（230 aa） Fragment：RCK domain (UNP residues 107-336)
Chain B 107–336（230 aa） Fragment：RCK domain (UNP residues 107-336)
Not recorded CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；295 K；6% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 0.2 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.84 Å R-free 0.253
4L75 Ca2+-bound D184N mutant MthK RCK domain at 2.4 Angstrom Deposited 2013-06-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 107–336（230 aa） Fragment：RCK domain (UNP residues 107-336)
Chain B 107–336（230 aa） Fragment：RCK domain (UNP residues 107-336)
Mutation：D184N Mutation：D184N CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；295 K；7% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 0.3 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.39 Å R-free 0.245
4L75 Ca2+-bound D184N mutant MthK RCK domain at 2.4 Angstrom Deposited 2013-06-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 107–336（230 aa） Fragment：RCK domain (UNP residues 107-336)
Chain D 107–336（230 aa） Fragment：RCK domain (UNP residues 107-336)
Mutation：D184N Mutation：D184N CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；295 K；7% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 0.3 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.39 Å R-free 0.245
4L75 Ca2+-bound D184N mutant MthK RCK domain at 2.4 Angstrom Deposited 2013-06-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain E 107–336（230 aa） Fragment：RCK domain (UNP residues 107-336)
Chain F 107–336（230 aa） Fragment：RCK domain (UNP residues 107-336)
Mutation：D184N Mutation：D184N CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；295 K；7% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 0.3 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.39 Å R-free 0.245
4L76 Ca2+-bound E212Q mutant MthK RCK domain Deposited 2013-06-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 107–336（230 aa） Fragment：RCK domain (UNP residues 107-336)
Chain B 107–336（230 aa） Fragment：RCK domain (UNP residues 107-336)
Mutation：E212Q Mutation：E212Q CA CALCIUM ION × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；295 K；8% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 0.2 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.99 Å R-free 0.263
4L76 Ca2+-bound E212Q mutant MthK RCK domain Deposited 2013-06-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 107–336（230 aa） Fragment：RCK domain (UNP residues 107-336)
Chain D 107–336（230 aa） Fragment：RCK domain (UNP residues 107-336)
Mutation：E212Q Mutation：E212Q CA CALCIUM ION × 5 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；295 K；8% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 0.2 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.99 Å R-free 0.263
4L76 Ca2+-bound E212Q mutant MthK RCK domain Deposited 2013-06-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain E 107–336（230 aa） Fragment：RCK domain (UNP residues 107-336)
Chain F 107–336（230 aa） Fragment：RCK domain (UNP residues 107-336)
Mutation：E212Q Mutation：E212Q CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；295 K；8% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 0.2 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.99 Å R-free 0.263
4QE7 Open MthK pore structure soaked in 10 mM Ba2+/100 mM Na+ Deposited 2014-05-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 19–99（81 aa） Fragment：UNP residues 19-99
Mutation：S68H, V77C BA BARIUM ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；3.0-3.5M 1,6-Hexandiol, 100mM Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.40 Å R-free 0.265
4QE9 Open MthK pore structure soaked in 10 mM Ba2+/100 mM K+ Deposited 2014-05-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 18–100（83 aa） Fragment：UNP residues 18-100
Mutation：S51H, V60C K POTASSIUM ION × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；3.0-3.5M 1,6-Hexandiol, 100mM HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.15 Å R-free 0.257
4RO0 Crystal structure of MthK gating ring in a ligand-free form Deposited 2014-10-27 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain B 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain C 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain D 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain a 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain b 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain c 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain d 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.4 M KCl, 0.1 M Tris and 20-25% PEGMME 350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 3.18 Å R-free 0.301
4RO0 Crystal structure of MthK gating ring in a ligand-free form Deposited 2014-10-27 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain E 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain F 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain G 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain H 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain e 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain f 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain g 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain h 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.4 M KCl, 0.1 M Tris and 20-25% PEGMME 350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 3.18 Å R-free 0.301
4RO0 Crystal structure of MthK gating ring in a ligand-free form Deposited 2014-10-27 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain I 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain J 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain K 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain L 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain i 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain j 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain k 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain l 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.4 M KCl, 0.1 M Tris and 20-25% PEGMME 350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 3.18 Å R-free 0.301
4RO0 Crystal structure of MthK gating ring in a ligand-free form Deposited 2014-10-27 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain M 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain N 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain O 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain P 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain m 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain n 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain o 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain p 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.4 M KCl, 0.1 M Tris and 20-25% PEGMME 350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 3.18 Å R-free 0.301
4RO0 Crystal structure of MthK gating ring in a ligand-free form Deposited 2014-10-27 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain Q 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain R 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain S 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain T 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain q 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain r 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain s 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Chain t 107–336（230 aa） Fragment：MthK RCK, UNP residues 107-336
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.4 M KCl, 0.1 M Tris and 20-25% PEGMME 350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 3.18 Å R-free 0.301
5BKI Blocker-free closed MthK channel in nanodisc Deposited 2021-03-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Chain E 1–336（336 aa）
Chain F 1–336（336 aa）
Chain G 1–336（336 aa）
Chain H 1–336（336 aa）
Not recorded CD CADMIUM ION × 12 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 4 K POTASSIUM ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
5BKJ TPeA-bound closed MthK channel in nanodisc Deposited 2021-03-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Chain E 1–336（336 aa）
Chain F 1–336（336 aa）
Chain G 1–336（336 aa）
Chain H 1–336（336 aa）
Not recorded PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 4 K POTASSIUM ION × 2 YQ1 1-(tripentyl-$l^{4}-azanyl)pentane × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5BKK bbTBA-bound closed MthK channel in nanodisc Deposited 2021-03-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Chain E 1–336（336 aa）
Chain F 1–336（336 aa）
Chain G 1–336（336 aa）
Chain H 1–336（336 aa）
Not recorded PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 2 K POTASSIUM ION × 3 YQ4 (~{R})-phenyl-[4-[(tributyl-$l^{4}-azanyl)methyl]phenyl]methanol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
6OLY Full-length MthK channel at 3.1 angstrom resolution Deposited 2019-04-17 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Mutation：M107I Mutation：M107I Mutation：M107I Mutation：M107I CA CALCIUM ION × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；300 K；28% PEG350-MME, 0.1M MES pH 6.5, 0.2 M CaCl2
Resolution 3.11 Å R-free 0.275
6U5N Calcium-bound MthK gating ring state 2 Deposited 2019-08-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Chain E 1–336（336 aa）
Chain F 1–336（336 aa）
Chain G 1–336（336 aa）
Chain H 1–336（336 aa）
Not recorded CA CALCIUM ION × 24 ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE；blotting for 2 seconds (blot force 0)
Resolution 3.20 Å
6U5P Calcium-bound MthK gating ring state 1 Deposited 2019-08-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Chain E 1–336（336 aa）
Chain F 1–336（336 aa）
Chain G 1–336（336 aa）
Chain H 1–336（336 aa）
Not recorded CA CALCIUM ION × 24 ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE；blotting for 2 seconds (blot force 0)
Resolution 3.30 Å
6U5R Calcium-bound MthK closed state Deposited 2019-08-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Chain E 1–336（336 aa）
Chain F 1–336（336 aa）
Chain G 1–336（336 aa）
Chain H 1–336（336 aa）
Not recorded CA CALCIUM ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE；blotting for 2 seconds (blot force 0)
Resolution 3.60 Å
6U68 Calcium-bound MthK open-inactivated state 1 Deposited 2019-08-29 Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Chain E 1–336（336 aa）
Chain F 1–336（336 aa）
Chain G 1–336（336 aa）
Chain H 1–336（336 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE；blotting for 2 s (blot force 0)
Resolution 4.50 Å
6U6D MthK closed state with EDTA Deposited 2019-08-29 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Chain E 1–336（336 aa）
Chain F 1–336（336 aa）
Chain G 1–336（336 aa）
Chain H 1–336（336 aa）
Not recorded K POTASSIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blotting for 2 s (blot force 0)
Resolution 3.60 Å
6U6E Calcium-bound MthK open-inactivated state 2 Deposited 2019-08-29 Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Chain E 1–336（336 aa）
Chain F 1–336（336 aa）
Chain G 1–336（336 aa）
Chain H 1–336（336 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
6U6H Calcium-bound MthK open-inactivated state 3 Deposited 2019-08-29 Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Chain E 1–336（336 aa）
Chain F 1–336（336 aa）
Chain G 1–336（336 aa）
Chain H 1–336（336 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
6U9P Wild-type MthK pore in ~150 mM K+ Deposited 2019-09-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 18–99（82 aa） Fragment：UNP residues 18-99
Not recorded K POTASSIUM ION × 20 HEZ HEXANE-1,6-DIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；1:1 protein (in 100 mM potassium chloride, 10 mM MOPS-KOH, pH 8.0) + 3.5-4.0 M 1,6-hexanediol, 100 mM MES-KOH, pH 6.75
Resolution 1.65 Å R-free 0.207
6U9T Wild-type MthK pore in 50 mM K+ Deposited 2019-09-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 18–99（82 aa） Fragment：UNP residues 18-99
Not recorded K POTASSIUM ION × 20 HEZ HEXANE-1,6-DIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；1:1 protein (in 100 mM sodium chloride, 1 mM potassium chloride, 10 mM MOPS, pH 8.0) + 3.5-4.0 M 1,6-hexanediol, 100 mM MES-NaOH, pH 6.75
Resolution 1.55 Å R-free 0.210
6U9Y Wild-type MthK pore in 11 mM K+ Deposited 2019-09-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 18–99（82 aa） Fragment：UNP residues 18-99
Mutation：UNP residues 18-99 K POTASSIUM ION × 20 HEZ HEXANE-1,6-DIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；1:1 protein (in 100 mM sodium chloride, 1 mM potassium chloride, 10 mM MOPS, pH 8.0) + 3.5-4.0 M 1,6-hexanediol, 100 mM MES-NaOH, 10 mM potassium chloride
Resolution 1.80 Å R-free 0.217
6U9Z Wild-type MthK pore in 6 mM K+ Deposited 2019-09-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 18–99（82 aa） Fragment：UNP residues 18-99
Not recorded K POTASSIUM ION × 20 HEZ HEXANE-1,6-DIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；protein (in 100 mM sodium chloride, 1 mM potassium chloride, 10 mM MOPS) + 3.5-4.0 M 1,6-hexanediol, 100 mM MES-NaOH, pH 6.75, 5 mM potassium chloride
Resolution 1.95 Å R-free 0.226
6UWN MthK N-terminal truncation RCK domain state 1 bound with calcium Deposited 2019-11-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Chain E 1–336（336 aa）
Chain F 1–336（336 aa）
Chain G 1–336（336 aa）
Chain H 1–336（336 aa）
Not recorded CA CALCIUM ION × 24 ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
6UX4 MthK N-terminal truncation RCK domain state 2 bound with calcium Deposited 2019-11-06 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Chain E 1–336（336 aa）
Chain F 1–336（336 aa）
Chain G 1–336（336 aa）
Chain H 1–336（336 aa）
Not recorded CA CALCIUM ION × 24 ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
6UX7 MthK N-terminal truncation state 1 bound with calcium Deposited 2019-11-06 Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Chain E 1–336（336 aa）
Chain F 1–336（336 aa）
Chain G 1–336（336 aa）
Chain H 1–336（336 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.70 Å
6UXA MthK N-terminal truncation state 2 bound with calcium Deposited 2019-11-07 Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Chain E 1–336（336 aa）
Chain F 1–336（336 aa）
Chain G 1–336（336 aa）
Chain H 1–336（336 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
6UXB MthK N-terminal truncation state 3 bound with calcium Deposited 2019-11-07 Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Chain E 1–336（336 aa）
Chain F 1–336（336 aa）
Chain G 1–336（336 aa）
Chain H 1–336（336 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.90 Å
8DJB MthK-A90L mutant in closed state with 0 Ca2+ Deposited 2022-06-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Chain E 1–336（336 aa）
Chain F 1–336（336 aa）
Chain G 1–336（336 aa）
Chain H 1–336（336 aa）
Not recorded K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE；BLOTTING FOR 2 S (BLOT FORCE 1)
Resolution 3.18 Å
8FZ7 TpeA bound closed MthK-A88F mutant in nanodisc Deposited 2023-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Chain E 1–336（336 aa）
Chain F 1–336（336 aa）
Chain G 1–336（336 aa）
Chain H 1–336（336 aa）
Not recorded PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 4 YQ1 1-(tripentyl-$l^{4}-azanyl)pentane × 1 K POTASSIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5
cryo-EM vitrification conditions Cryogen ETHANE；BLOTTING FOR 2 S (BLOT FORCE 0)
Resolution 2.88 Å
9Z2V Ca2+-bound MthK WT in lipid nanodiscs composed of 22:1PC (75%) and POPG (25%) Deposited 2025-11-05 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–336（336 aa）
Chain B 1–336（336 aa）
Chain C 1–336（336 aa）
Chain D 1–336（336 aa）
Chain E 1–336（336 aa）
Chain F 1–336（336 aa）
Chain G 1–336（336 aa）
Chain H 1–336（336 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8.5；20 mM HEPES-KOH pH 8.5 100 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å

47 other PDB entries and 76 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name MTHK_METTH
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–336； UniProt 1–336 Author chain B； PDBConstruct 1–336； UniProt 1–336 Author chain C； PDBConstruct 1–336； UniProt 1–336 Author chain D； PDBConstruct 1–336； UniProt 1–336 Author chain E； PDBConstruct 1–336； UniProt 1–336 Author chain F； PDBConstruct 1–336； UniProt 1–336 Author chain G； PDBConstruct 1–336； UniProt 1–336 Author chain H； PDBConstruct 1–336； UniProt 1–336

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 9z2m

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 9z2m
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2. Structure Basics 2. Structure Basics

Entry ID entry_id9z2m
Deposition date deposition_date2025-11-05
Structure title titleCa2+-bound MthK WT in lipid nanodiscs composed of 14:1PC (75%) and POPG (25%)
Keywords keywordsIon channel, Calcium-activated potassium channel, MEMBRANE PROTEIN, TRANSPORT PROTEIN; TRANSPORT PROTEIN
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier46.45 Å
Radius of gyration Rg (electron density) rg_electron46.16 Å
Forward intensity I(0) i0489192000.00
Molecular weight molecular_weight158020.0 kDa
Excluded volume excluded_volume187090 ų
Envelope volume envelope_volume363390 ų
Hydration-shell volume shell_volume67576 ų
Envelope diameter envelope_diameter138.0 Å
Shell Rg shell_rg49.52 Å
Envelope Rg envelope_rg43.59 Å
Shape Rg shape_rg46.17 Å
Total Rg total_rg46.30 Å
Total atoms total_atoms11283
Residues n_residues2104
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax131.1 Å
Rg (real space) rg_real46.12 Å
Rg uncertainty (real space) rg_real_error0.91 Å
I(0) (real space) i0_real4.8920e+08
I(0) uncertainty (real space) i0_real_error8.4150e+06
Rg (reciprocal space) rg_reciprocal46.45 Å
I(0) (reciprocal space) i0_reciprocal489400000.0000
Solution quality estimate total_estimate0.8695
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary65.5 Å
Skewness Skewness skewness-0.042
Kurtosis Kurtosis kurtosis-0.593
Angular range angular_range— – 0.1700 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha29870000.0000
Real-space data points n_real_points35
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.966; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.404
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (1)

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8. Citations (1)

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9. Files and Curves (10)