Current Protein Identity：O14727
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1C15 SOLUTION STRUCTURE OF APAF-1 CARD Deposited 1999-07-20 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–97（97 aa）
Fragment：CASPASE RECRUITMENT DOMAIN
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；300 K；Ionic strength (raw mmCIF value) 1；Pressure AMBIENT
NMR sample composition
1MM 15N LABELED APAF-1 CARD, 20 MM PHOSPHATE BUFFER, 50 MM NACL
NMR sample composition
1MM 13C LABELED APAF-1 CARD, 20 MM PHOSPHATE BUFFER, 50 MM NACL
NMR sample composition
1MM NON-LABELED APAF-1 CARD, 20 MM PHOSPHATE BUFFER, 50 MM NACL
NMR sample composition
1MM 13C,15N LABELED, 80%DEUTERATED APAF-1 CARD, 20 MM PHOSPHATE BUFFER, 50 MM
NACL
|
Resolution not provided |
| 1CWW SOLUTION STRUCTURE OF THE CASPASE RECRUITMENT DOMAIN (CARD) FROM APAF-1 Deposited 1999-08-26 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–97（97 aa）
Fragment：CASPASE RECRUITMENT DOMAIN
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.7；30 K
NMR measurement conditions
pH 6.7；30 K
NMR measurement conditions
pH 6.7；30 K
NMR sample composition
UNLABELLED 1.5MM APAF-1 CARD 20MM SODIUM PHOSPHATE PH 6.7 75MM NACL 2MM DITHIOTHREITOL
NMR sample composition
U-15N 1.5MM APAF-1 CARD 20MM SODIUM PHOSPHATE PH 6.7 75MM NACL 2MM DITHIOTHREITOL
NMR sample composition
U-15N; U-13C 1.5MM APAF-1 CARD 20MM SODIUM PHOSPHATE PH 6.7 75MM NACL 2MM DITHIOTHREITOL
|
Resolution not provided |
| 1CY5 CRYSTAL STRUCTURE OF THE APAF-1 CARD Deposited 1999-08-31 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–97（97 aa）
Fragment：CASPASE RECRUITMENT DOMAIN
|
Not recorded | ZN ZINC ION × 5 BME BETA-MERCAPTOETHANOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；290 K；HANGING DROP VAPOR DIFFUSION WITH EQUAL VOLUMES OF PROTEIN AND RESERVOIR SOLUTIONS. PROTEIN SOLUTION: 5-20 MG/ML
PROTEIN IN 0.02 MOLAR TRIS (PH 8.0-8.5) , 0.1-0.2 MOLAR SODIUM CHLORIDE. 0.005 MOLAR 2-MERCAPTOETHANOL RESERVOIR
SOLUTION: 8-20% (W/V) PEG 8000, 0.2 MOLAR TRIS (PH 7.5-8.0), 0.05-0.2 MOLAR ZINC ACETATE., VAPOR DIFFUSION, HANGING
DROP, temperature 17K
|
Resolution 1.30 Å R-free 0.199 |
| 1Z6T Structure of the apoptotic protease-activating factor 1 bound to ADP Deposited 2005-03-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–591（591 aa）
Fragment：Apaf-1, residues 1-591
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；276 K；HEPES, ammonium acetate, PEG-3350, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 276K, pH 7.10
|
Resolution 2.21 Å R-free 0.244 |
| 1Z6T Structure of the apoptotic protease-activating factor 1 bound to ADP Deposited 2005-03-23 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–591（591 aa）
Fragment：Apaf-1, residues 1-591
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；276 K；HEPES, ammonium acetate, PEG-3350, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 276K, pH 7.10
|
Resolution 2.21 Å R-free 0.244 |
| 1Z6T Structure of the apoptotic protease-activating factor 1 bound to ADP Deposited 2005-03-23 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
1–591（591 aa）
Fragment：Apaf-1, residues 1-591
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；276 K；HEPES, ammonium acetate, PEG-3350, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 276K, pH 7.10
|
Resolution 2.21 Å R-free 0.244 |
| 1Z6T Structure of the apoptotic protease-activating factor 1 bound to ADP Deposited 2005-03-23 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
1–591（591 aa）
Fragment：Apaf-1, residues 1-591
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；276 K；HEPES, ammonium acetate, PEG-3350, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 276K, pH 7.10
|
Resolution 2.21 Å R-free 0.244 |
| 2P1H Rapid Folding and Unfolding of Apaf-1 CARD Deposited 2007-03-05 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–92（92 aa）
Fragment：CARD domain (Residues 1-92)
|
Not recorded | ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.1；291 K；200 mM Tris-HCl, pH 6.1, 200 mM zinc acetate, 17% PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 1.59 Å R-free 0.297 |
| 2YGS CARD DOMAIN FROM APAF-1 Deposited 1999-05-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–92（92 aa）
Fragment：CASPASE RECRUITMENT DOMAIN (CARD)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6；pH 6.0
|
Resolution 1.60 Å R-free 0.236 |
| 3J2T An improved model of the human apoptosome Deposited 2012-12-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count |
Chain A
1–1248（1248 aa）
Chain B
1–1248（1248 aa）
Chain C
1–1248（1248 aa）
Chain D
1–1248（1248 aa）
Chain E
1–1248（1248 aa）
Chain F
1–1248（1248 aa）
Chain G
1–1248（1248 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
low salt HEPES buffer；pH 7.5；20mM HEPES, 10mM KCl, 1.5mM MgCl2, 1mM EDTA, 1mM EGTA, 1mM DTT
cryo-EM vitrification conditions
Blot for 2 seconds before plunging；77 K；Cryogen ETHANE；Blot for 2 seconds before plunging (FEI VITROBOT MARK III)
|
Resolution 9.50 Å |
| 3JBT Atomic structure of the Apaf-1 apoptosome Deposited 2015-10-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count |
Chain A
1–1248（1248 aa）
Chain C
1–1248（1248 aa）
Chain E
1–1248（1248 aa）
Chain G
1–1248（1248 aa）
Chain I
1–1248（1248 aa）
Chain K
1–1248（1248 aa）
Chain M
1–1248（1248 aa）
|
Not recorded | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 7 MG MAGNESIUM ION × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
20 mM HEPES (pH 7.5), 10 mM KCl, 1.5 mM MgCl2, 1 mM EDTA, 1 mM DTT；pH 7.5；20 mM HEPES (pH 7.5), 10 mM KCl, 1.5 mM MgCl2, 1 mM EDTA, 1 mM DTT
cryo-EM vitrification conditions
Blot for 2.5 seconds before plunging；Cryogen ETHANE
|
Resolution 3.80 Å |
| 3YGS APAF-1 CARD IN COMPLEX WITH PRODOMAIN OF PROCASPASE-9 Deposited 1999-05-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1–95（95 aa）
Fragment：CASPASE RECRUITMENT DOMAIN (CARD)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 2.50 Å R-free 0.299 |
| 4RHW Crystal structure of Apaf-1 CARD and caspase-9 CARD complex Deposited 2014-10-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
1–97（97 aa）
Fragment：card domain
Chain B
1–97（97 aa）
Fragment：card domain
Chain C
1–97（97 aa）
Fragment：card domain
Chain D
1–97（97 aa）
Fragment：card domain
|
Not recorded | SO4 SULFATE ION × 7 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；291 K；13% PEG 3000, 0.2M ammonium sulfate, 0.1M MES (pH5.5), VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.10 Å R-free 0.226 |
| 5JUY Active human apoptosome with procaspase-9 Deposited 2016-05-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count |
Chain A
1–1248（1248 aa）
Chain B
1–1248（1248 aa）
Chain C
1–1248（1248 aa）
Chain D
1–1248（1248 aa）
Chain E
1–1248（1248 aa）
Chain F
1–1248（1248 aa）
Chain G
1–1248（1248 aa）
|
Not recorded | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 7 HEC HEME C × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；Buffer A
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 5WVC Structure of the CARD-CARD disk Deposited 2016-12-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
1–95（95 aa）
Fragment：UNP RESIDUES 1-95
Chain C
1–95（95 aa）
Fragment：UNP RESIDUES 1-95
Chain E
1–95（95 aa）
Fragment：UNP RESIDUES 1-95
|
Not recorded | IOD IODIDE ION × 11 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.2 M NH4I, 20% PEG 3350
|
Resolution 2.99 Å R-free 0.228 |
| 5WVE Apaf-1-Caspase-9 holoenzyme Deposited 2016-12-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 25 PDB declaration: 25-meric（25） Consistent with protein count |
Chain A
1–1248（1248 aa）
Chain C
1–1248（1248 aa）
Chain E
1–1248（1248 aa）
Chain G
1–1248（1248 aa）
Chain I
1–1248（1248 aa）
Chain K
1–1248（1248 aa）
Chain M
1–1248（1248 aa）
Chain O
1–102（102 aa）
Fragment：CARD domain, UNP residues 1-102
Chain P
1–102（102 aa）
Fragment：CARD domain, UNP residues 1-102
Chain Q
1–102（102 aa）
Fragment：CARD domain, UNP residues 1-102
Chain R
1–102（102 aa）
Fragment：CARD domain, UNP residues 1-102
Chain W
1–102（102 aa）
Fragment：CARD domain, UNP residues 1-102
Chain X
1–102（102 aa）
Fragment：CARD domain, UNP residues 1-102
|
Not recorded | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 7 MG MAGNESIUM ION × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.40 Å |
| 8XQK The Crystal Structure of Apaf from Biortus. Deposited 2024-01-05 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–97（97 aa）
|
Not recorded | PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；1.6M Na/KPO4 pH6.5
|
Resolution 2.85 Å R-free 0.283 |
| 8XQK The Crystal Structure of Apaf from Biortus. Deposited 2024-01-05 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–97（97 aa）
|
Not recorded | PO4 PHOSPHATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；1.6M Na/KPO4 pH6.5
|
Resolution 2.85 Å R-free 0.283 |
| 8XQK The Crystal Structure of Apaf from Biortus. Deposited 2024-01-05 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
1–97（97 aa）
|
Not recorded | PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；1.6M Na/KPO4 pH6.5
|
Resolution 2.85 Å R-free 0.283 |
| 8XQK The Crystal Structure of Apaf from Biortus. Deposited 2024-01-05 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
1–97（97 aa）
|
Not recorded | PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；1.6M Na/KPO4 pH6.5
|
Resolution 2.85 Å R-free 0.283 |