Current Protein Identity:O95391
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 5XJC Cryo-EM structure of the human spliceosome just prior to exon ligation at 3.6 angstrom Deposited 2017-04-30 | Assembly 1 Protein–RNA Heteromer;Protein × 46 PDB declaration: 50-meric(50) Consistent with all polymers |
Chain Z
1–586(586 aa)
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 11 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6ICZ Cryo-EM structure of a human post-catalytic spliceosome (P complex) at 3.0 angstrom Deposited 2018-09-07 | Assembly 1 Protein–RNA Heteromer;Protein × 47 PDB declaration: 51-meric(51) Consistent with all polymers |
Chain Z
1–586(586 aa)
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 9 ZN ZINC ION × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 6QDV Human post-catalytic P complex spliceosome Deposited 2019-01-03 | Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 54-meric(54) Consistent with all polymers |
Chain c
1–586(586 aa)
|
Not recorded | MG MAGNESIUM ION × 7 K POTASSIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 IHP INOSITOL HEXAKISPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE;3 uL sample was applied to the grid, left for 25s, then blotted for 2.5-3.5s and immediately plunged into liquid ethane.
|
Resolution 3.30 Å |
| 7W5A The cryo-EM structure of human pre-C*-II complex Deposited 2021-11-29 | Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 54-meric(54) Consistent with all polymers |
Chain 1
1–586(586 aa)
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 9 ZN ZINC ION × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 7W5B The cryo-EM structure of human C* complex Deposited 2021-11-29 | Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers |
Chain 1
1–586(586 aa)
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 9 ZN ZINC ION × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |
| 8C6J Human spliceosomal PM5 C* complex Deposited 2023-01-12 | Assembly 1 Protein–RNA Heteromer;Protein × 58 PDB declaration: 63-meric(63) Consistent with all polymers |
Chain h
1–586(586 aa)
|
Not recorded | K POTASSIUM ION × 2 MG MAGNESIUM ION × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9FMD Integrative model of the human post-catalytic spliceosome (P-complex) Deposited 2024-06-05 | Assembly 1 Protein–RNA Heteromer;Protein × 58 PDB declaration: 63-meric(63) Consistent with all polymers |
Chain U
1–586(586 aa)
|
Not recorded | MG MAGNESIUM ION × 6 K POTASSIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 IHP INOSITOL HEXAKISPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |