当前蛋白身份：P06129
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1NQE OUTER MEMBRANE COBALAMIN TRANSPORTER (BTUB) FROM E. COLI 提交 2003-01-21 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
21–614（594 aa）
|
未记录 | MG MAGNESIUM ION × 1 C8E (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE × 7 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.6；293 K；PEG 3350, magnesium acetate, bis-tris, n-octyl tetraoxyethylene , pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
分辨率 2.00 Å R-free 0.232 |
| 1NQF OUTER MEMBRANE COBALAMIN TRANSPORTER (BTUB) FROM E. COLI, METHIONINE SUBSTIUTION CONSTRUCT FOR SE-MET SAD PHASING 提交 2003-01-21 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
21–614（594 aa）
|
突变：W317M, K319M, T321M, W516M, I518M, Y520M 非标准单体：是（mmCIF未提供具体位点） | MG MAGNESIUM ION × 1 C8E (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.6；293 K；PEG3350, MAGNESIUM ACETATE, BIS-TRIS, N-OCTYL TETRAOXYETHELYENE, tris-(2-carboxyethyl)phosphine hydrochloride , pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
分辨率 2.70 Å R-free 0.288 |
| 1NQG OUTER MEMBRANE COBALAMIN TRANSPORTER (BTUB) FROM E. COLI, WITH BOUND CALCIUM 提交 2003-01-21 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
21–614（594 aa）
|
未记录 | CA CALCIUM ION × 4 C8E (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.6；293 K；PEG 3350, MAGNESIUM ACETATE, BIS-TRIS, N-OCTYL TETRAOXYETHYLENE, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
分辨率 3.31 Å R-free 0.259 |
| 1NQH OUTER MEMBRANE COBALAMIN TRANSPORTER (BTUB) FROM E. COLI, WITH BOUND CALCIUM AND CYANOCOBALAMIN (VITAMIN B12) SUBSTRATE 提交 2003-01-21 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
21–614（594 aa）
|
未记录 | CA CALCIUM ION × 4 CNC CYANOCOBALAMIN × 1 C8E (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.6；293 K；PEG 3350, MAGNESIUM ACETATE, BIS-TRIS, N-OCTYL TETRAOXYETHYLENE, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
分辨率 3.10 Å R-free 0.265 |
| 1UJW Structure of the complex between BtuB and Colicin E3 Receptor binding domain 提交 2003-08-12 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
21–614（594 aa）
|
未记录 | GP1 2-amino-2-deoxy-1-O-phosphono-alpha-D-glucopyranose × 2 LIM 3-OXO-PENTADECANOIC ACID × 2 LDA LAURYL DIMETHYLAMINE-N-OXIDE × 7 AAE ACETOACETIC ACID × 1 GOL GLYCEROL × 8 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.75 Å R-free 0.293 |
| 2GSK Structure of the BtuB:TonB Complex 提交 2006-04-26 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
25–614（590 aa）
|
未记录 | CA CALCIUM ION × 2 CNC CYANOCOBALAMIN × 1 LDA LAURYL DIMETHYLAMINE-N-OXIDE × 4 OCT N-OCTANE × 3 HEX HEXANE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.1；288 K；30% (v/v) PEG 300, 0.1M sodium acetate , pH 5.1, VAPOR DIFFUSION, SITTING DROP, temperature 288K, pH 5.10
|
分辨率 2.10 Å R-free 0.251 |
| 2GUF In meso crystal structure of the cobalamin transporter, BtuB 提交 2006-04-29 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
21–614（594 aa）
|
未记录 | MPG [(Z)-octadec-9-enyl] (2R)-2,3-bis(oxidanyl)propanoate × 11 FMT FORMIC ACID × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 1.95 Å R-free 0.225 |
| 2YSU Structure of the complex between BtuB and Colicin E2 receptor binding domain 提交 2007-04-04 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
21–614（594 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 3.50 Å R-free 0.315 |
| 3M8B Crystal structure of spin-labeled BtuB V10R1 in the apo state 提交 2010-03-17 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
21–614（594 aa）
|
突变：V10R1A | C8E (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE × 7 MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 1 MG MAGNESIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.6；290 K；2.5% PEG3350,20 mM Bis Tris, 150 mM Magnesium acetate, 10 mM C8E4, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
分辨率 2.44 Å R-free 0.248 |
| 3M8D Crystal structure of spin-labeled BtuB V10R1 with bound calcium and cyanocobalamin 提交 2010-03-17 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
21–614（594 aa）
|
突变：V10R1A | CNC CYANOCOBALAMIN × 1 C8E (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE × 6 MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 1 CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.6；290 K；2.5% PEG3350, 150 mM calcium chloride, 20 mM Bis tris, 10 mM C8E4, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
分辨率 2.44 Å R-free 0.275 |
| 3RGM Crystal structure of spin-labeled BtuB T156R1 提交 2011-04-08 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
21–614（594 aa）
|
突变：T156R1 | C8E (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE × 6 MG MAGNESIUM ION × 9 MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.6；290 K；3.0% PEG3350, 200 mM magnesium acetate, 10mM C8E4, 20 mM Bis Tris pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
分辨率 2.60 Å R-free 0.254 |
| 3RGN Crystal structure of spin-labeled BtuB W371R1 提交 2011-04-08 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
21–614（594 aa）
|
突变：W371R1 | C8E (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE × 7 MG MAGNESIUM ION × 3 MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.6；290 K；3.0% PEG3350, 200 mM magnesium acetate, 10 mM C8E4, 20 mM Bis Tris pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
分辨率 2.30 Å R-free 0.250 |
| 7NSU ColicinE9 intact translocation complex 提交 2021-03-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 F
21–614（594 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.9
cryo-EM玻璃化条件
冷冻剂 ETHANE；3 uL of diluted translocon preparation were applied on freshly glow discharged grids coated with graphene oxide (as described in https://doi.org/10.1038/s41594-019-0355-2); after 30sec waiting grids were blotted for 8-10 using -10 force and plunge frozen in liquid ethane
|
分辨率 4.70 Å |