Current Protein Identity：P0A334
New Search
Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1BL8 POTASSIUM CHANNEL (KCSA) FROM STREPTOMYCES LIVIDANS Deposited 1998-07-23 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
23–119（97 aa）
Chain B
23–119（97 aa）
Chain C
23–119（97 aa）
Chain D
23–119（97 aa）
|
Mutation：L90C Mutation：L90C Mutation：L90C Mutation：L90C | K POTASSIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；ONE-TO-MIXTURE OF PROTEIN SOLUTION AND RESERVOIR (200 MM CACL2, 100 MM HEPES PH 7.5, 48% PEG 400).
|
Resolution 3.20 Å R-free 0.290 |
| 1F6G POTASSIUM CHANNEL (KCSA) FULL-LENGTH FOLD Deposited 2000-06-21 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–160（160 aa）
Fragment：FULL-LENGTH CHANNEL
Chain B
1–160（160 aa）
Fragment：FULL-LENGTH CHANNEL
Chain C
1–160（160 aa）
Fragment：FULL-LENGTH CHANNEL
Chain D
1–160（160 aa）
Fragment：FULL-LENGTH CHANNEL
|
Mutation：CYS SCANNING: 5-24, 120-160 Mutation：CYS SCANNING: 5-24, 120-160 Mutation：CYS SCANNING: 5-24, 120-160 Mutation：CYS SCANNING: 5-24, 120-160 | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.2；293 K；Ionic strength (raw mmCIF value) 50-100 mM；Pressure ambient
NMR sample composition
50-100 uM KcsA, PBS pH 7.2, reconstituted into asolectin vesicles at a 1:500 protein:lipid ratio (molar) | 100% H2O
|
Resolution not provided |
| 1J95 KCSA potassium channel with TBA (tetrabutylammonium) and potassium Deposited 2001-05-23 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–125（125 aa）
Fragment：Residues 1-125
Chain B
1–125（125 aa）
Fragment：Residues 1-125
Chain C
1–125（125 aa）
Fragment：Residues 1-125
Chain D
1–125（125 aa）
Fragment：Residues 1-125
|
Mutation：L90C Mutation：L90C Mutation：L90C Mutation：L90C | K POTASSIUM ION × 4 TBA TETRABUTYLAMMONIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG 400, HEPES, POTASSIUM CHLORIDE, CALCIUM CHLORIDE, DETERGENT LDAO, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.322 |
| 1JQ1 POTASSIUM CHANNEL (KCSA) OPEN GATE MODEL Deposited 2001-08-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
86–119（34 aa）
Fragment：INNER TRANSMEMBRANE SEGMENT (residues 86-119)
Chain B
86–119（34 aa）
Fragment：INNER TRANSMEMBRANE SEGMENT (residues 86-119)
Chain C
86–119（34 aa）
Fragment：INNER TRANSMEMBRANE SEGMENT (residues 86-119)
Chain D
86–119（34 aa）
Fragment：INNER TRANSMEMBRANE SEGMENT (residues 86-119)
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；150 K；Ionic strength (raw mmCIF value) 20 mM CITRATE PHOSPHATE；Pressure 1
NMR measurement conditions
pH 4；150 K；Ionic strength (raw mmCIF value) 20 mM CITRATE PHOSPHATE；Pressure 1
NMR sample composition
1.0 MG/ML MIXED WITH METHANETHIOSULFONATE SPIN LABEL | THE SAMPLES WERE RECONSTITUTED INTO ASOLECTIN LIPOSOMES AT A 1:400 PROTEIN:LIPID RATIO
|
Resolution not provided |
| 1JQ2 POTASSIUM CHANNEL (KCSA) OPEN GATE MODEL Deposited 2001-08-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
86–119（34 aa）
Fragment：INNER TRANSMEMBRANE SEGMENT (residues 86-119)
Chain B
86–119（34 aa）
Fragment：INNER TRANSMEMBRANE SEGMENT (residues 86-119)
Chain C
86–119（34 aa）
Fragment：INNER TRANSMEMBRANE SEGMENT (residues 86-119)
Chain D
86–119（34 aa）
Fragment：INNER TRANSMEMBRANE SEGMENT (residues 86-119)
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；150 K；Ionic strength (raw mmCIF value) 20 mM CITRATE PHOSPHATE；Pressure 1
NMR measurement conditions
pH 4；150 K；Ionic strength (raw mmCIF value) 20 mM CITRATE PHOSPHATE；Pressure 1
NMR sample composition
1.0 MG/ML MIXED WITH METHANETHIOSULFONATE SPIN LABEL | THE SAMPLES WERE RECONSTITUTED INTO ASOLECTIN LIPOSOMES AT A 1:400 PROTEIN:LIPID RATIO
|
Resolution not provided |
| 1JVM KCSA POTASSIUM CHANNEL WITH TBA (TETRABUTYLAMMONIUM) AND RUBIDIUM Deposited 2001-08-30 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–125（125 aa）
Fragment：Residues 1-125
Chain B
1–125（125 aa）
Fragment：Residues 1-125
Chain C
1–125（125 aa）
Fragment：Residues 1-125
Chain D
1–125（125 aa）
Fragment：Residues 1-125
|
Mutation：P2A,L90C Mutation：P2A,L90C Mutation：P2A,L90C Mutation：P2A,L90C | RB RUBIDIUM ION × 3 TBA TETRABUTYLAMMONIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG400,calcium chloride, Hepes, potassium chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.302 |
| 1JVM KCSA POTASSIUM CHANNEL WITH TBA (TETRABUTYLAMMONIUM) AND RUBIDIUM Deposited 2001-08-30 | Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–125（125 aa）
Fragment：Residues 1-125
Chain B
1–125（125 aa）
Fragment：Residues 1-125
Chain C
1–125（125 aa）
Fragment：Residues 1-125
Chain D
1–125（125 aa）
Fragment：Residues 1-125
|
Mutation：P2A,L90C Mutation：P2A,L90C Mutation：P2A,L90C Mutation：P2A,L90C | RB RUBIDIUM ION × 6 TBA TETRABUTYLAMMONIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG400,calcium chloride, Hepes, potassium chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.302 |
| 1K4C Potassium Channel KcsA-Fab complex in high concentration of K+ Deposited 2001-10-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–124（124 aa）
Fragment：potassium channel KcsA
|
Mutation：P2A, L90C | F09 NONAN-1-OL × 4 K POTASSIUM ION × 28 DGA DIACYL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.4；298 K；PEG 400, Magnesium Acetate, Sodium Acetate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.233 |
| 1K4D Potassium Channel KcsA-Fab complex in low concentration of K+ Deposited 2001-10-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–124（124 aa）
Fragment：potassium channel KcsA
|
Mutation：P2A, L90C | K POTASSIUM ION × 8 NA SODIUM ION × 4 DGA DIACYL GLYCEROL × 4 F09 NONAN-1-OL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.4；298 K；PEG 400,Magnesium Acetate, Sodium Acetate , pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.30 Å R-free 0.235 |
| 1R3I potassium channel KcsA-Fab complex in Rb+ Deposited 2003-10-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–124（124 aa）
|
Mutation：P2A,L90C | F09 NONAN-1-OL × 4 RB RUBIDIUM ION × 16 DGA DIACYL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.4；293 K；PEG400, sodium acetate, magnesium acetate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.236 |
| 1R3J potassium channel KcsA-Fab complex in high concentration of Tl+ Deposited 2003-10-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–124（124 aa）
|
Mutation：P2A,L90C | F09 NONAN-1-OL × 4 TL THALLIUM (I) ION × 20 DGA DIACYL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.4；293 K；PEG400, sodium acetate, magnesium acetate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.90 Å R-free 0.228 |
| 1R3K potassium channel KcsA-Fab complex in low concentration of Tl+ Deposited 2003-10-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–124（124 aa）
|
Mutation：P2A,L90C | DGA DIACYL GLYCEROL × 4 TL THALLIUM (I) ION × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.4；293 K；PEG400, sodium acetate, magnesium acetate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.273 |
| 1R3L potassium channel KcsA-Fab complex in Cs+ Deposited 2003-10-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–124（124 aa）
|
Mutation：P2A,L90C | F09 NONAN-1-OL × 4 CS CESIUM ION × 20 DGA DIACYL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.4；293 K；PEG400, sodium acetate, magnesium acetate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.41 Å R-free 0.249 |
| 1ZWI Structure of mutant KcsA potassium channel Deposited 2005-06-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
22–123（102 aa）
Fragment：residues 22-123
|
Not recorded | F09 NONAN-1-OL × 1 K POTASSIUM ION × 7 DGA DIACYL GLYCEROL × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.50 Å R-free 0.256 |
| 2A9H NMR structural studies of a potassium channel / charybdotoxin complex Deposited 2005-07-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain A
1–132（132 aa）
Chain B
1–132（132 aa）
Chain C
1–132（132 aa）
Chain D
1–132（132 aa）
|
Mutation：Q58A, T61S, R64D, F103Y, T107F, L110V Mutation：Q58A, T61S, R64D, F103Y, T107F, L110V Mutation：Q58A, T61S, R64D, F103Y, T107F, L110V Mutation：Q58A, T61S, R64D, F103Y, T107F, L110V | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.5；315 K；Pressure ambient
NMR sample composition
1 mM KcsA, 2H and selectively 13C-methyl labeled in 20 mM sodium phosphate (pH 7.5), 5 mM KCl, 1 mM DTT, and 80 mM foscholine-12, 90% H2O/10% D2O or 100% D2O | 90% H2O/10% D2O or 100% D2O
|
Resolution not provided |
| 2ATK Structure of a mutant KcsA K+ channel Deposited 2005-08-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
1–124（124 aa）
|
Not recorded | K POTASSIUM ION × 7 F09 NONAN-1-OL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.4；300 K；PEG400, KCl, MgAcetate, NaAcetate, pH 5.40, VAPOR DIFFUSION, SITTING DROP, temperature 300K
|
Resolution 2.50 Å R-free 0.261 |
| 2BOB Potassium channel KcsA-Fab complex in thallium with tetrabutylammonium (TBA) Deposited 2005-04-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–124（124 aa）
Fragment：RESIDUES 1-124
|
Mutation：YES | TL THALLIUM (I) ION × 4 CO COBALT (II) ION × 4 TBA TETRABUTYLAMMONIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.4；PEG 400, MAGNESIUM ACETATE, SODIUM ACETATE, pH 5.40
|
Resolution 2.76 Å R-free 0.251 |
| 2BOC Potassium channel KcsA-Fab complex in thallium with tetraethylarsonium (TEAs) Deposited 2005-04-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–124（124 aa）
Fragment：RESIDUES 1-124
|
Mutation：YES | TL THALLIUM (I) ION × 16 CO COBALT (II) ION × 4 T1A TETRAETHYLARSONIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.4；PEG 400, MAGNESIUM ACETATE, SODIUM ACETATE, pH 5.40
|
Resolution 3.01 Å R-free 0.250 |
| 2DWD crystal structure of KcsA-FAB-TBA complex in Tl+ Deposited 2006-08-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
22–124（103 aa）
Fragment：residues 22-124
|
Mutation：L90C | F09 NONAN-1-OL × 4 TL THALLIUM (I) ION × 16 L2C (2S)-3-HYDROXY-2-(NONANOYLOXY)PROPYL LAURATE × 4 TBA TETRABUTYLAMMONIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；18-25% PEG 400, 50mM MG(AC)2, 50mM NaAc(pH 5) or Na cacodylate(pH 6) or HEPES(pH 7), pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.263 |
| 2DWE Crystal structure of KcsA-FAB-TBA complex in Rb+ Deposited 2006-08-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
22–124（103 aa）
Fragment：residues 22-124
|
Mutation：L90C | RB RUBIDIUM ION × 12 L2C (2S)-3-HYDROXY-2-(NONANOYLOXY)PROPYL LAURATE × 4 F09 NONAN-1-OL × 4 TBA TETRABUTYLAMMONIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；18-25% PEG 400, 50mM MG(AC)2, 50mM NaAc(pH 5) or Na cacodylate(pH 6) or HEPES(pH 7), pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.266 |
| 2HJF Potassium channel kcsa-fab complex with tetrabutylammonium (TBA) Deposited 2006-06-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
22–124（103 aa）
|
Mutation：L90C | K POTASSIUM ION × 16 TBA TETRABUTYLAMMONIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.2；295 K；Cryoprotected to 45% PEG, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
Resolution 2.90 Å R-free 0.284 |
| 2HVJ Crystal structure of KcsA-Fab-TBA complex in low K+ Deposited 2006-07-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–124（124 aa）
|
Mutation：P2A, L90C | F09 NONAN-1-OL × 4 K POTASSIUM ION × 8 L2C (2S)-3-HYDROXY-2-(NONANOYLOXY)PROPYL LAURATE × 4 TBA TETRABUTYLAMMONIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.5；293 K；18-25% PEG 400, 50mM Mg(Ac)2, 50mM NaAc (pH5) or Na Cacodylate (pH6) or Hepes (PH 7), pH 6.5, VAPOR DIFFUSION, temperature 293K
|
Resolution 2.75 Å R-free 0.246 |
| 2HVK crystal structure of the KcsA-Fab-TBA complex in high K+ Deposited 2006-07-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–124（124 aa）
|
Mutation：P2A, L90C | F09 NONAN-1-OL × 4 K POTASSIUM ION × 24 L2C (2S)-3-HYDROXY-2-(NONANOYLOXY)PROPYL LAURATE × 4 TBA TETRABUTYLAMMONIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.5；293 K；18-25% PEG 400, 50mM Mg(Ac)2, 50mM NaAc (pH5) or Na Cacodylate (pH6) or Hepes (PH 7), pH 6.5, VAPOR DIFFUSION, temperature 293K
|
Resolution 1.90 Å R-free 0.245 |
| 2IH1 Ion selectivity in a semi-synthetic K+ channel locked in the conductive conformation Deposited 2006-09-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
3–122（120 aa）
|
Mutation：S69A, V70C Non-standard monomer：Yes (specific site not provided by mmCIF) | K POTASSIUM ION × 20 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.5；293 K；Crystals grown in 150 mM KCl were transferred to a solution containing 1 mM KCl + 149 mM NaCl prior to cryoprotection, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 5.50
|
Resolution 2.40 Å R-free 0.251 |
| 2IH3 Ion selectivity in a semi-synthetic K+ channel locked in the conductive conformation Deposited 2006-09-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
3–122（120 aa）
|
Mutation：S69A, V70C Non-standard monomer：Yes (specific site not provided by mmCIF) | K POTASSIUM ION × 28 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6；293 K；20-25% PEG 400, 50 mM Magnesium acetate, 150 mM KCl, pH 6.0. Crystals were cryoprotected by increasing PEG concentration to 40%, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.72 Å R-free 0.242 |
| 2ITC Potassium Channel KcsA-Fab complex in Sodium Chloride Deposited 2006-10-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–124（124 aa）
Fragment：residues 1-124
|
Mutation：L90C | NA SODIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；21% PEG400, 50 mM MgAcetate, 150 mM NaCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.20 Å R-free 0.265 |
| 2ITD Potassium Channel KcsA-Fab complex in Barium Chloride Deposited 2006-10-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–124（124 aa）
Fragment：RESIDUES 1-124
|
Mutation：L90C | BA BARIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.2；293 K；21% PEG400, 50 mM MgAcetate, 5 mM BaCl2, 150 mM NaCl, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.70 Å R-free 0.261 |
| 2JK5 Potassium Channel KcsA in complex with Tetrabutylammonium in high K Deposited 2008-08-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–124（124 aa）
Fragment：RESIDUES 1-124
|
Not recorded | CO COBALT (II) ION × 4 K POTASSIUM ION × 24 F09 NONAN-1-OL × 4 L2C (2S)-3-HYDROXY-2-(NONANOYLOXY)PROPYL LAURATE × 4 TBA TETRABUTYLAMMONIUM ION × 4 HP6 HEPTANE × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.4；18-25%PEG400, 50 MM SODIUM ACETATE, 50 MM MAGNESIUM ACETATE, pH 5.4
|
Resolution 2.40 Å R-free 0.249 |
| 2NLJ Potassium Channel KcsA(M96V)-Fab complex in KCl Deposited 2006-10-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–124（124 aa）
Fragment：RESIDUES 1-124
|
Mutation：L90C, M96V | DGA DIACYL GLYCEROL × 4 K POTASSIUM ION × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；PEG400, Magnesium Acetate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.52 Å R-free 0.255 |
| 2P7T Crystal Structure of KcsA mutant Deposited 2007-03-20 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain C
22–124（103 aa）
|
Not recorded | K POTASSIUM ION × 24 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；pH 5.2-5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.05 Å R-free 0.234 |
| 2P7T Crystal Structure of KcsA mutant Deposited 2007-03-20 | Assembly 3 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
22–124（103 aa）
|
Not recorded | F09 NONAN-1-OL × 4 K POTASSIUM ION × 24 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；pH 5.2-5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.05 Å R-free 0.234 |
| 2QTO An anisotropic model for potassium channel KcsA Deposited 2007-08-02 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
23–119（97 aa）
Chain B
23–119（97 aa）
Chain C
23–119（97 aa）
Chain D
23–119（97 aa）
|
Not recorded | K POTASSIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；200 MM CACL2, 100 MM HEPES PH 7.5, 48% PEG 400
|
Resolution 3.20 Å R-free 0.272 |
| 2W0F Potassium Channel KcsA-Fab Complex with Tetraoctylammonium Deposited 2008-08-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–124（124 aa）
Fragment：RESIDUES 1-124
|
Not recorded | CO COBALT (II) ION × 4 F09 NONAN-1-OL × 4 DGA DIACYL GLYCEROL × 4 HX0 N,N,N-trioctyloctan-1-aminium × 4 K POTASSIUM ION × 28 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.4；18-25% PEG400, 50 MM SODIUM ACETATE, 50 MM MAGNESIUM ACETATE, pH 5.4
|
Resolution 2.40 Å R-free 0.241 |
| 3EFF The Crystal Structure of Full-Length KcsA in its Closed Conformation Deposited 2008-09-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain K
22–160（139 aa）
Fragment：UNP residues 22-160
Chain L
22–160（139 aa）
Fragment：UNP residues 22-160
Chain M
22–160（139 aa）
Fragment：UNP residues 22-160
Chain N
22–160（139 aa）
Fragment：UNP residues 22-160
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.6；298 K；340 mM (NH4)2SO4, 11% PEG4000, 100 mM Na3C6H5O7 pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 3.80 Å R-free 0.331 |
| 3F5W KcsA Potassium channel in the open-inactivated state with 32 A opening at T112 Deposited 2008-11-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
21–124（104 aa）
|
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q | K POTASSIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；25% PEG 400, 50 mM Sodium acetate, 50mM magnesium acetate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 3.30 Å R-free 0.265 |
| 3F7V KcsA Potassium channel in the open-inactivated state with 23 A opening at T112 Deposited 2008-11-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
21–124（104 aa）
|
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q | K POTASSIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 3.20 Å R-free 0.276 |
| 3F7V KcsA Potassium channel in the open-inactivated state with 23 A opening at T112 Deposited 2008-11-10 | Assembly 2 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
21–124（104 aa）
|
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q | K POTASSIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 3.20 Å R-free 0.276 |
| 3F7Y KcsA Potassium channel in the partially open state with 17 A opening at T112 Deposited 2008-11-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
21–124（104 aa）
Fragment：UNP residues 21-124
|
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q | K POTASSIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 3.40 Å R-free 0.274 |
| 3F7Y KcsA Potassium channel in the partially open state with 17 A opening at T112 Deposited 2008-11-10 | Assembly 2 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
21–124（104 aa）
Fragment：UNP residues 21-124
|
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q | K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 3.40 Å R-free 0.274 |
| 3FB5 KcsA potassium channel in the partially open state with 14.5 A opening at T112 Deposited 2008-11-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
21–124（104 aa）
Fragment：UNP residues 21-124
|
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q | K POTASSIUM ION × 24 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, pH 5-6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.80 Å R-free 0.252 |
| 3FB5 KcsA potassium channel in the partially open state with 14.5 A opening at T112 Deposited 2008-11-18 | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
21–124（104 aa）
Fragment：UNP residues 21-124
|
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q | K POTASSIUM ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, pH 5-6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.80 Å R-free 0.252 |
| 3FB6 KcsA Potassium channel in the partially open state with 16 A opening at T112 Deposited 2008-11-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
21–124（104 aa）
Fragment：UNP residues 21-124
|
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q | K POTASSIUM ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, pH 5-6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 3.00 Å R-free 0.255 |
| 3FB6 KcsA Potassium channel in the partially open state with 16 A opening at T112 Deposited 2008-11-18 | Assembly 2 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
21–124（104 aa）
Fragment：UNP residues 21-124
|
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q | K POTASSIUM ION × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, pH 5-6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 3.00 Å R-free 0.255 |
| 3FB7 Open KcsA potassium channel in the presence of Rb+ ion Deposited 2008-11-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
21–124（104 aa）
Fragment：UNP residues 21-124
|
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q | RB RUBIDIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 3.30 Å R-free 0.255 |
| 3FB7 Open KcsA potassium channel in the presence of Rb+ ion Deposited 2008-11-18 | Assembly 2 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
21–124（104 aa）
Fragment：UNP residues 21-124
|
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q | RB RUBIDIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 3.30 Å R-free 0.255 |
| 3FB8 KcsA Potassium channel in the open-conductive state with 20 A opening at T112 in the presence of Rb+ ion Deposited 2008-11-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
21–124（104 aa）
Fragment：UNP residues 21-124
|
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q | RB RUBIDIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 3.40 Å R-free 0.304 |
| 3FB8 KcsA Potassium channel in the open-conductive state with 20 A opening at T112 in the presence of Rb+ ion Deposited 2008-11-18 | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
21–124（104 aa）
Fragment：UNP residues 21-124
|
Mutation：H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q | RB RUBIDIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 3.40 Å R-free 0.304 |
| 3GB7 Potassium Channel KcsA-Fab complex in Li+ Deposited 2009-02-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–124（124 aa）
|
Mutation：P2A, L90C | NI NICKEL (II) ION × 4 DGA DIACYL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；24% PEG 400, 100 mM MES, 50 mM Magnesium Acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.85 Å R-free 0.285 |
| 3HPL KcsA E71H-F103A mutant in the closed state Deposited 2009-06-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
1–124（124 aa）
Fragment：UNP residues 1-124
|
Mutation：L90C,E71H,F103A | K POTASSIUM ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；20-25% PEG 400, 50 mM magnesium acetate, 50 mM sodium acetate, pH 5.0-6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 3.20 Å R-free 0.269 |
| 3IFX Crystal structure of the Spin-labeled KcsA mutant V48R1 Deposited 2009-07-26 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–123（123 aa）
Fragment：Pore domain: UNP residues 1-124
Chain B
1–123（123 aa）
Fragment：Pore domain: UNP residues 1-124
Chain C
1–123（123 aa）
Fragment：Pore domain: UNP residues 1-124
Chain D
1–123（123 aa）
Fragment：Pore domain: UNP residues 1-124
|
Mutation：V48C Mutation：V48C Mutation：V48C Mutation：V48C | K POTASSIUM ION × 2 MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 4 TBA TETRABUTYLAMMONIUM ION × 1 | Not declared |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；200 mM CaCl2, 150 mM KCl, 100 mM HEPES, 19-49% PEG 400, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.56 Å R-free 0.302 |
| 3IFX Crystal structure of the Spin-labeled KcsA mutant V48R1 Deposited 2009-07-26 | Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–123（123 aa）
Fragment：Pore domain: UNP residues 1-124
Chain B
1–123（123 aa）
Fragment：Pore domain: UNP residues 1-124
Chain C
1–123（123 aa）
Fragment：Pore domain: UNP residues 1-124
Chain D
1–123（123 aa）
Fragment：Pore domain: UNP residues 1-124
|
Mutation：V48C Mutation：V48C Mutation：V48C Mutation：V48C | K POTASSIUM ION × 4 MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 8 TBA TETRABUTYLAMMONIUM ION × 2 | Not declared |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；200 mM CaCl2, 150 mM KCl, 100 mM HEPES, 19-49% PEG 400, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.56 Å R-free 0.302 |
| 3IGA Potassium Channel KcsA-Fab complex in Li+ and K+ Deposited 2009-07-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–124（124 aa）
|
Mutation：P2A, L90C | NI NICKEL (II) ION × 4 DGA DIACYL GLYCEROL × 4 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.75 Å R-free 0.273 |
| 3OGC KcsA E71A variant in presence of Na+ Deposited 2010-08-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
2–124（123 aa）
Fragment：UNP RESIDUES 2-124
|
Mutation：E71A | NA SODIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG 400, MgAcetate, MES, pH 6.5, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP
|
Resolution 3.80 Å R-free 0.283 |
| 3OR6 On the structural basis of modal gating behavior in K+channels - E71Q Deposited 2010-09-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
22–124（103 aa）
Fragment：UNP RESIDUES 22-124
|
Mutation：E71Q | K POTASSIUM ION × 28 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.268 |
| 3OR7 On the structural basis of modal gating behavior in K+channels - E71I Deposited 2010-09-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
22–124（103 aa）
Fragment：UNP RESIDUES 22-124
|
Mutation：E71I | K POTASSIUM ION × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.30 Å R-free 0.271 |
| 3PJS Mechanism of Activation Gating in the Full-Length KcsA K+ Channel Deposited 2010-11-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain K
22–160（139 aa）
Chain L
22–160（139 aa）
Chain M
22–160（139 aa）
Chain N
22–160（139 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.2M Na/K phosphate, 0.1M Bis-Tris propane pH 7.5, 10% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.80 Å R-free 0.332 |
| 3STL KcsA potassium channel mutant Y82C with Cadmium bound Deposited 2011-07-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
22–124（103 aa）
|
Mutation：Y82C | K POTASSIUM ION × 24 CD CADMIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；293 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.263 |
| 3STZ KcsA potassium channel mutant Y82C with nitroxide spin label Deposited 2011-07-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
23–124（102 aa）
|
Mutation：Y82C | MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 4 K POTASSIUM ION × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；293 K；20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.285 |
| 4LBE Structure of KcsA with R122A mutation Deposited 2013-06-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
2–124（123 aa）
|
Mutation：R122A | F09 NONAN-1-OL × 4 DGA DIACYL GLYCEROL × 4 K POTASSIUM ION × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；PEG 400, MgAcetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.75 Å R-free 0.202 |
| 4LCU Structure of KcsA with E118A mutation Deposited 2013-06-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
2–124（123 aa）
|
Not recorded | K POTASSIUM ION × 24 DGA DIACYL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；PEG 400, MgAcetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.75 Å R-free 0.216 |
| 4MSW Y78 ester mutant of KcsA in high K+ Deposited 2013-09-18 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain C
22–76（55 aa）
Fragment：UNP residues 22-124,UNP residues 22-124
Chain C
78–124（47 aa）
Fragment：UNP residues 22-124,UNP residues 22-124
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | DGA DIACYL GLYCEROL × 4 K POTASSIUM ION × 24 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；PEG400, Magnesium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 2.06 Å R-free 0.229 |
| 4UUJ POTASSIUM CHANNEL KCSA-FAB WITH TETRAHEXYLAMMONIUM Deposited 2014-07-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
22–124（103 aa）
|
Not recorded | F09 NONAN-1-OL × 16 IND INDOLE × 4 K POTASSIUM ION × 16 DGA DIACYL GLYCEROL × 4 CO COBALT (II) ION × 4 XA7 TETRAHEXYL AMMONNIUM × 8 PO4 PHOSPHATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.4；CRYSTALLIZATION CONDITIONS: 18-25% PEG400, 50 MM SODIUM REMARK 280 ACETATE, 50 MM MAGNESIUM ACETATE, pH 5.4
|
Resolution 2.40 Å R-free 0.248 |
| 5E1A Structure of KcsA with L24C/R117C mutations Deposited 2015-09-29 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain C
4–124（121 aa）
Fragment：UNP residues 4-124
|
Mutation：L24C, R117C | K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.75；293 K；PEG 400, MES, Magnesium Acetate
|
Resolution 3.40 Å R-free 0.257 |
| 5EBL KcsA T75G in the Conductive State Deposited 2015-10-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–125（125 aa）
Fragment：UNP residues 1-125
|
Mutation：T75G | F09 NONAN-1-OL × 4 DGA DIACYL GLYCEROL × 4 K POTASSIUM ION × 24 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；PEG 400
|
Resolution 2.30 Å R-free 0.242 |
| 5EBM KcsA T75G mutant in the nonconductive state Deposited 2015-10-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–125（125 aa）
Fragment：UNP residues 1-125
|
Mutation：T75G | DGA DIACYL GLYCEROL × 4 F09 NONAN-1-OL × 4 K POTASSIUM ION × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；PEG 400
|
Resolution 2.50 Å R-free 0.251 |
| 5EBW KcsA with G77ester mutation Deposited 2015-10-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–123（123 aa）
Fragment：UNP residues 1-123
|
Mutation：G77(GOA) Non-standard monomer：Yes (specific site not provided by mmCIF) | F09 NONAN-1-OL × 4 DGA DIACYL GLYCEROL × 4 K POTASSIUM ION × 24 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；PEG400
|
Resolution 2.30 Å R-free 0.249 |
| 5EC1 KcsA with V76ester mutation Deposited 2015-10-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–125（125 aa）
Fragment：UNP residues 1-125
|
Mutation：V76ester Non-standard monomer：Yes (specific site not provided by mmCIF) | F09 NONAN-1-OL × 4 DGA DIACYL GLYCEROL × 4 K POTASSIUM ION × 24 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；PEG400
|
Resolution 2.75 Å R-free 0.268 |
| 5EC2 KcsA with V76ester+G77dA mutations Deposited 2015-10-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–125（125 aa）
Fragment：UNP residues 1-125
|
Mutation：V76ester,G77dA Non-standard monomer：Yes (specific site not provided by mmCIF) | F09 NONAN-1-OL × 4 DGA DIACYL GLYCEROL × 4 K POTASSIUM ION × 24 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；PEG400
|
Resolution 2.30 Å R-free 0.259 |
| 5J9P KcsA in vitro Deposited 2016-04-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
22–117（96 aa）
Fragment：UNP residues 22-117
|
Not recorded | K POTASSIUM ION × 24 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG400
|
Resolution 2.85 Å R-free 0.266 |
| 5VK6 Open conformation of KcsA non-inactivating E71A mutant Deposited 2017-04-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
26–121（96 aa）
|
Not recorded | F09 NONAN-1-OL × 4 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 K POTASSIUM ION × 28 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG400, 50 mM magnesium acetate, 50 mM sodium acetate
|
Resolution 2.25 Å R-free 0.213 |
| 5VKE Open conformation of KcsA deep-inactivated Deposited 2017-04-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
26–121（96 aa）
|
Not recorded | F09 NONAN-1-OL × 4 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 K POTASSIUM ION × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG400, 50 mM magnesium acetate, 50 mM sodium acetate
|
Resolution 2.37 Å R-free 0.228 |
| 5VKH Closed conformation of KcsA Y82A-F103A mutant Deposited 2017-04-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
22–124（103 aa）
|
Not recorded | F09 NONAN-1-OL × 4 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 K POTASSIUM ION × 24 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG400 , 50 mM magnesium acetate, 50 mM sodium acetate
|
Resolution 2.25 Å R-free 0.203 |
| 6NFU Structure of the KcsA-G77A mutant or the 2,4-ion bound configuration of a K+ channel selectivity filter. Deposited 2018-12-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
22–124（103 aa）
|
Mutation：G77C, L90C | F09 NONAN-1-OL × 4 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG400, magnesium acetate, sodium acetate
|
Resolution 2.09 Å R-free 0.224 |
| 6NFV Structure of the KcsA-G77C mutant or the 2,4-ion bound configuration of a K+ channel selectivity filter. Deposited 2018-12-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
22–124（103 aa）
|
Mutation：G77C, L90C | F09 NONAN-1-OL × 4 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG400 , magnesium acetate, sodium acetate
|
Resolution 2.13 Å R-free 0.243 |
| 6PA0 Structure of the G77A mutant in Sodium Chloride Deposited 2019-06-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
22–124（103 aa）
|
Mutation：G77A, L90C | F09 NONAN-1-OL × 4 DGA DIACYL GLYCEROL × 4 NA SODIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；22-26 % PEG400 (v/v), 50 mM magnesium acetate, 50 mM sodium acetate (pH 4.8.-5.4)
|
Resolution 2.05 Å R-free 0.233 |
| 6W0A Open-gate KcsA soaked in 1 mM BaCl2 Deposited 2020-02-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
28–120（93 aa）
|
Not recorded | BA BARIUM ION × 4 K POTASSIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
|
Resolution 3.24 Å R-free 0.241 |
| 6W0B Open-gate KcsA soaked in 2 mM BaCl2 Deposited 2020-02-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
28–120（93 aa）
|
Not recorded | BA BARIUM ION × 4 K POTASSIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
|
Resolution 3.60 Å R-free 0.236 |
| 6W0C Open-gate KcsA soaked in 4 mM BaCl2 Deposited 2020-02-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
28–120（93 aa）
|
Not recorded | BA BARIUM ION × 4 K POTASSIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
|
Resolution 3.56 Å R-free 0.248 |
| 6W0D Open-gate KcsA soaked in 5 mM BaCl2 Deposited 2020-02-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
28–120（93 aa）
|
Not recorded | BA BARIUM ION × 4 K POTASSIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
|
Resolution 3.64 Å R-free 0.248 |
| 6W0E Open-gate KcsA soaked in 10 mM BaCl2 Deposited 2020-02-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
28–120（93 aa）
|
Not recorded | BA BARIUM ION × 4 K POTASSIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
|
Resolution 3.51 Å R-free 0.250 |
| 6W0F Closed-gate KcsA soaked in 0mM KCl/5mM BaCl2 Deposited 2020-02-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
22–124（103 aa）
|
Not recorded | K POTASSIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
|
Resolution 2.40 Å R-free 0.270 |
| 6W0G Closed-gate KcsA soaked in 1mM KCl/5mM BaCl2 Deposited 2020-02-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
22–124（103 aa）
|
Mutation：L90C | K POTASSIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
|
Resolution 2.60 Å R-free 0.245 |
| 6W0H Closed-gate KcsA soaked in 5mM KCl/5mM BaCl2 Deposited 2020-02-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
22–124（103 aa）
|
Mutation：L90C | K POTASSIUM ION × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
|
Resolution 2.60 Å R-free 0.239 |
| 6W0I Closed-gate KcsA soaked in 10mM KCl/5mM BaCl2 Deposited 2020-02-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
22–124（103 aa）
|
Mutation：L90C | K POTASSIUM ION × 24 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
|
Resolution 2.33 Å R-free 0.232 |
| 6W0J Closed-gate KcsA incubated in BaCl2/NaCl Deposited 2020-02-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
22–124（103 aa）
|
Mutation：L90C | BA BARIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5
|
Resolution 2.50 Å R-free 0.218 |
| 7M2H Structural Snapshots of Intermediates in the Gating of a K+ Channel Deposited 2021-03-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
3–125（123 aa）
|
Not recorded | F09 NONAN-1-OL × 4 K POTASSIUM ION × 24 DGA DIACYL GLYCEROL × 4 TBA TETRABUTYLAMMONIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.25；293 K；50 mM MES, pH 6.25, 28% PEG400, 50 mM magnesium acetate
|
Resolution 2.64 Å R-free 0.268 |
| 7M2H Structural Snapshots of Intermediates in the Gating of a K+ Channel Deposited 2021-03-16 | Assembly 2 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain F
3–125（123 aa）
|
Not recorded | F09 NONAN-1-OL × 4 K POTASSIUM ION × 24 DGA DIACYL GLYCEROL × 4 TBA TETRABUTYLAMMONIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.25；293 K；50 mM MES, pH 6.25, 28% PEG400, 50 mM magnesium acetate
|
Resolution 2.64 Å R-free 0.268 |
| 7M2I Structural Snapshots of Intermediates in the Gating of a K+ Channel Deposited 2021-03-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
26–116（91 aa）
|
Not recorded | F09 NONAN-1-OL × 4 1EM (1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE × 4 K POTASSIUM ION × 24 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；50 mM MES, pH 6.25, 28% PEG400, 50 mM magnesium acetate
|
Resolution 2.69 Å R-free 0.251 |
| 7M2J Structural Snapshots of Intermediates in the Gating of a K+ Channel Deposited 2021-03-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
26–116（91 aa）
|
Not recorded | K POTASSIUM ION × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；33% PEG400, 100 mM MES, pH 6.5, 100 mM calcium chloride
|
Resolution 3.20 Å R-free 0.305 |
| 7MDJ The structure of KcsA in complex with a synthetic Fab Deposited 2021-04-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
1–124（124 aa）
|
Not recorded | K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；50 mM sodium acetate/50 mM ammonium acetate,
50 mM magnesium acetate,
25% PEG 400.
|
Resolution 2.75 Å R-free 0.265 |
| 7MHR KcsA E71V closed gate with K+ Deposited 2021-04-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
3–124（122 aa）
|
Mutation：E71V, L90C | K POTASSIUM ION × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；50 mM sodium acetate pH 5.5, 50 mM magnesium acetate, 25% PEG 400
|
Resolution 2.77 Å R-free 0.203 |
| 7MHX KcsA E71V closed gate with Ba2+ Deposited 2021-04-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
2–124（123 aa）
|
Mutation：P2A, E71V, L90C | BA BARIUM ION × 4 DGA DIACYL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；50 mM sodium acetate pH 5.5, 50 mM magnesium acetate, 25% PEG 400
|
Resolution 2.85 Å R-free 0.244 |
| 7MJT KcsA open gate E71V mutant with Barium Deposited 2021-04-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
26–121（96 aa）
|
Mutation：A28C, E71V, L90C, R117Q, E118C, E120Q, R121Q | BA BARIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；50 mM sodium/ammonium acetate pH 5.5, 50 mM Magnesium acetate, 25% PG 400
|
Resolution 3.30 Å R-free 0.270 |
| 7MK6 KcsA open gate E71V mutant with sodium Deposited 2021-04-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
26–121（96 aa）
|
Mutation：A28C, E71V, L90C, R117Q, E118C, E120Q, R121Q | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；50 mM sodium/ammonium acetate pH 5.5, 50 mM Magnesium acetate, 25% PEG 400
|
Resolution 3.10 Å R-free 0.253 |
| 7MUB KcsA Open gate E71V mutant in Potassium Deposited 2021-05-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
26–121（96 aa）
|
Mutation：A28C, E71V, L90C, R117Q, E118C, E120Q, R121Q | K POTASSIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；50 mM Sodium acetate/Ammonium acetate, 50 mM Magnesium acetate, 25% PEG 400
|
Resolution 3.00 Å R-free 0.260 |
| 7RP0 Structural Snapshots of Intermediates in the Gating of a K+ Channel Deposited 2021-08-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain C
22–124（103 aa）
Fragment：UNP residues 22-124
|
Mutation：Y82A | F09 NONAN-1-OL × 4 K POTASSIUM ION × 16 TBA TETRABUTYLAMMONIUM ION × 4 DGA DIACYL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.25；293 K；50 mM MES, pH 6.25, 50 mM magnesium acetate, 27% PEG400
|
Resolution 2.48 Å R-free 0.271 |
| 8THN KcsA M96V mutant with Y78ester in High K+ Deposited 2023-07-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
22–124（103 aa）
|
Mutation：M96V, Y78ester Non-standard monomer：Yes (specific site not provided by mmCIF) | K POTASSIUM ION × 4 TBA TETRABUTYLAMMONIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.25；291 K；27% PEG 400 (v/v), 50 mM magnesium acetate, 50 mM MES pH 6.25
|
Resolution 2.90 Å R-free 0.276 |